The investigation of intracapsular ligaments of knee joint is important in the clinical setup to make accurate diagnosis and surgical procedures of the knee joint. ¹ There has been a substantial rise in the information about the arthroscopic management of the meniscal injuries. ² The menisci can be easily injured and it is hard to repair them. ³ However, due to advanced technologies, the torn menisci can be repaired by arthroscopic surgery and the best modality of treatment available is the meniscal allograft transplantation, especially for the young symptomatic patients. ⁴ At present, arthroscopy can involve meniscal transplantation, scaffold implantation and insertion of healing substances at the torn area of the meniscus. ⁵ Meniscectomy can be performed arthroscopically and it is followed by synthetic or allograft meniscal transplantation as this prevents friction between the tibia and the femur. ⁶ It was opined that successful meniscus transplant surgery relies on the accuracy in the dimensions matching between the recipient and donor meniscus. ⁷ Precision of the measurements between the allograft and the recipient knee is important for successful function of the meniscus after the transplantation surgery. If the graft size is small, forces acting on the meniscus are increased, which may lead to misbalancing of the femoral condyles and compartment overload. ⁸ If the allograft is larger, there will be no load bearing and the forces will be increased over the articular cartilages of the knee joint, which causes degenerative changes in the knee joint. ⁷ It was reported that only 5-10% of the mismatch of the graft size is accepted. ⁹

The morphometric data of menisci will also assist the biomechanical research and hypothesis, which explores the functional relationships of the femoro-tibial articulation. ¹⁰ This has implications for tissue engineering of the meniscus and offering the normal joint function. There are clinical studies, which suggest that radiological measurements of the menisci need to be compared with the menisci from cadaveric specimens to confirm and make the radiological method as the gold standard. ⁷ The morphological information about the sizes of the meniscus can benefit in differentiating the normal meniscus from the discoid and tiny meniscus. ¹¹ The bucket handle meniscal tear decreases the width, so it is important to differentiate the torn meniscus from the normal meniscus in the radio diagnosis. It was reported that, alterations in the width and thickness of meniscus can give clue to the type of its injury and impact. ¹² Smillie ¹³ described that the thickness and width of the menisci are important to understand the possibility, location and type of meniscal injury. The morphological variability between the meniscus of medial and lateral compartment is important to understand the meniscal damage. ¹³

The operating surgeons should ask the tissue providing banks for the allograft, which matches with the dimension of the torn meniscus. ⁷ In this context, the normative data about the breadth and thickness of the menisci may help in choosing the accurate meniscal graft. Due to these clinical implications, the objective of the present research was to measure the breadth and depth of the meniscus in corpses and provide normative data of this sample population. The objective was to correlate the dimensions over the three different regions of meniscus and over the right- and left-sided specimens.

This study was a descriptive cross-sectional observational research, performed at the department of anatomy of Kasturba Medical College, Manipal Academy of Higher Education, Manipal, India. All the strobe guidelines for the cross-sectional study are met and the details are available at https://figshare.com/articles/dataset/Strobe_Checklist/21605694. The study participants were formalin fixed human adult cadaveric knee joints of unknown age and gender. Only embalmed undissected lower extremity specimens were included in this study. The specimens, which exhibited the visible pathological changes and congenital anomalies were excluded. The study duration was one year from 23.08.2021 to 23.08.2022. The sample size was 50 adult cadaveric knee joints; amongst them 25 were right- sided and 25 left-sided specimens. The sample size of this present study was calculated by the formula 'n = Zα ² x σ ²/d ²', where Z _α= 1.96 at 95% confidence level, σ = standard deviation, d= 1% absolute precision with 95% confidence level and 80 % power with 1% absolute precision, the sample size comes to be 100. The reference article used to calculate the sample size was El-Aasar et al. ¹⁴

In total, 100 menisci were studied (50 medial menisci and 50 lateral menisci). The gender of the specimens were not taken into consideration. The cadaveric lower limbs were frozen, which were initially embalmed with 10% formalin. After dissecting the skin, fascia and muscles, a longitudinal cut was given on the medial and lateral sideways of the knee joint to open the capsule. The ligamentum patellae, tibial and fibular collateral ligaments were cut by giving a transverse incision. The knee capsule and cruciate ligaments were cut for better access to the menisci, while the femoral and tibial condyles were separated from the soft tissues, which exposed the whole menisci. The knee joints which showed pathological changes and were found damaged were excluded from the present study. The knee joints exhibiting congenital anomalies and tears of the menisci were also disqualified from the present research. This present research followed the guidelines of the international ethical standards as per Padulo et al. ¹⁵

The menisci were allocated into three parts by measuring with a thread and the points were marked. These three regions were named as the anterior, middle and posterior one third ( Figure 1) and the measurements of thickness and width were performed at their middle parts. This was approximately corresponding to the 1 o clock, 3 o clock and 5 o clock positions. The measurements were performed with the vernier caliper of 0.02 mm accuracy. At the mid-point of each 1/3, the caliper was positioned between the outer and inner borders of the menisci to measure the width. The thickness was measured at the same points by placing the caliper between the upper and lower surfaces of the menisci at the outer edge only. All the dimensions were completed by the same researcher to avoid the inter observer variation and three readings were taken for each measurement to prevent the intra-observer error.

The data were tabulated in the microsoft excel (RRID:SCR_016137) document and presented as mean ± standard deviation. The online SPSS (RRID:SCR_002865) program (version number 25) was utilized for the analysis. The calculation of values and statistical analysis between the right versus left sides and medial versus lateral tibial partitions were done by using the paired samples ‘t’ test and repeated measures ANOVA (online software SPSS (RRID:SCR_002865), version number 25, https://www.ibm.com/products/spss-statistics-gradpack). The ‘p’ value less than 0.05 was considered as significant (α = 0.05). The protocol of this research is available online at https://www.protocols.io/view/thickness-and-width-of-the-menisci-of-adult-knee-j-cjh2uj8e.

The thickness and width of the medial meniscus at the 3 different regions are tabulated and compared among the right and left sides ( Table 1). Table 2 shows the morphometric data and comparison of lateral meniscus among the left and right knee joints. The present study observed that there was no significance in this comparison for the thickness and width between the corresponding right and left knee menisci, in both medial and lateral compartments (p>0.05).

The medial and lateral menisci were compared to each other irrespective of their side and the comparison is given in Table 3. Related to the peripheral circumference thickness, the anterior one-third region of medial meniscus is thicker than the lateral (p<0.05). But in the middle and posterior third regions ( Table 3), medial and lateral menisci are of relatively same thickness (p>0.05). In the anterior and middle third regions, the lateral meniscus has additional width than the medial (p<0.05). But in the posterior one-third region, medial meniscus was wider (p<0.05), than the lateral meniscus ( Table 3).

The posterior one-third region of the medial meniscus is thicker ( Figure 2) than its anterior and middle one-thirds (p<0.05). The middle and anterior one-third regions are of the same relative thickness (p>0.05). The posterior one-third region of the medial meniscus has greater width ( Figure 2) than its own anterior and middle one-thirds (p<0.05). The anterior and middle one-third regions were of the same relative width (p>0.05).

The statistics did not reveal the differences (p>0.05) amongst the thicknesses of anterior, middle and posterior parts of lateral meniscus ( Figure 3). However, the ventral and dorsal one-third regions of lateral meniscus ( Figure 3) are of the same relative width (p>0.05) and they are greater than the middle one-third region (p<0.05).

After taking the average from all the three regions, the breadth of medial and lateral meniscus was 9.69 ± 1.89 mm and 9.61 ± 1.77 mm, respectively. The average thickness of the medial and lateral meniscus was 4.39 ± 0.67 mm and 4.17 ± 0.58 mm, correspondingly. With respect to the average dimensions of width and thickness, the statistical significance was not observed (p>0.05) between the medial and lateral meniscus.

Meniscal damages are among the most frequent harms in the sports medicine, which can cause pain, functional disability and swelling around the knee joint. ¹⁶ The meniscal tears occur due to the bending forces and rotational knee injuries. They can also occur due to degeneration or spontaneously due to the progressive structural failure, which is known as the meniscal fatigue injury. ¹⁷ Knee arthroscopy is the gold standard investigation, which can simultaneously treat the meniscus disorders. ³ The graft sizing, precise positioning and fixation are the three prerequisites for successful allograft meniscal transplantation. ¹⁸ In allograft meniscal transplantation, which is performed for the torn menisci, inaccurate size of the graft can lead to its failure and cause complications like knee joint degeneration. Successful arthroscopic meniscal transplantation and regeneration depend on the accuracy in the shape and size of the allograft in comparison to the torn meniscus. This requires the morphometric data of the menisci as the mismatching leads to surgical failure and degenerative changes in the knee joint. ^{9 , 19 – 20} The normative data of dimensions of the meniscus are essential while planning the surgical interventions of the knee joint. This can help in differentiating the discoid meniscus from a normal one. The discoid meniscus is an anomaly and develops due to the variability in the morphogenesis. ²¹ It was reported that, variability in the thickness and width of menisci can lead to the meniscal injury. ¹² Meniscectomy has long-term complications like joint degeneration, and it is advisable to do conservative surgery by repairing the torn meniscus, but for this, grafting is required. Accurate size of the meniscal allograft is required for the success of the arthroscopic surgery in terms of the joint function and healing. The correct sizing of the graft requires detailed knowledge about the dimensions of the menisci.

In this perspective, the present cadaveric study has provided normative data about the thickness and width of the meniscus in this sample population of Indians. The complete set of data of the present study was compared with the previous studies from other populations and is represented in Table 4. The observations of the present study agree with the findings of Didio, ²² Braz and Silva ²³ that the posterior region was the broadest among all the three regions in the medial meniscus. In our previous study from fetal knee joints also, it was found that the posterior part was the wider of the medial meniscus. ²⁴ In this research from Indian samples, significant difference was not obtained among the anterior and middle third widths. This was similar to our finding in the previous study in fetal medial meniscus. ²⁴ However, Braz and Silva ²³ stated that the mid body of the medial meniscus has greater width than the anterior one-third.

Almeida et al., ¹² Braz and Silva ²³ reported no statistical significance in the comparison of the width between the three regions of the lateral meniscus. We observed that the anterior and posterior one-third regions of lateral meniscus were of the same relative width. However, these two regions were wider than the middle one-third region. In the fetal lateral meniscus, it was a different observation, where the middle region was the widest. ²⁴

In vivo radiological research by Bloecker et al. ¹⁰ revealed that medial meniscus has greater thickness than the lateral. However, there was no significance in the differences with respect to the breadth. Testut and Latarjet, ²⁵ and Didio ²² reported that, meniscus of the medial side is wider than the lateral, when the average measurement was taken. Miller ²⁶ also had the same observations in his study. However, the present study does not agree with these opinions as we didn’t find differences between the medial and lateral compartments, when the average was taken from all the three regions.

Figueroa et al. ²⁷ observed that, the anterior region of lateral meniscus is wider than the medial meniscus. In this study, dissimilarity was not observed between the medial and lateral meniscus, average widths. Dhananjaya et al. ²⁸ reported the in vivo measurements of the menisci in south Indian population. In their study, it was obvious that the lateral meniscus had higher breadths at the anterior and middle regions than its counterpart. They also detected that the posterior region of the medial meniscus had higher width than the lateral meniscus in the same region. They noted that the medial and lateral menisci were wider at their posterior region than the anterior region. The middle one-third region was relatively lesser in width.

However, the present study agrees with the finding of Braz and Silva ²³ that the anterior region of the medial meniscus has greater thickness than that of the same part of the lateral meniscus. Our study agrees with the Bloecker et al. ¹⁰ that the thickness of the middle part of the medial and lateral menisci was the same. We further observed that the thickness of the dorsal part of the medial and lateral menisci was the same. This is not as per the findings of Bloecker et al., ¹⁰ where posterior region of medial meniscus was thicker than its counterpart. The study of fetal knee joints did not reveal statistically significant variance with respect to the thickness of the menisci of the medial and lateral compartments. ²⁴ However, the lateral meniscus had greater width than its counterpart in the medial compartment in the fetal tibia.

Rico and Ayala ²⁹ opined that the middle part of the medial menisci is prone to tears (51%), which is followed by the posterior one-third (39%). This was supported by the anatomical studies, and it was hypothesized that thicker region of the meniscus is more prone for tears. Braz and Silva ²³ stated that, middle region of the medial meniscus was thicker, which is trailed by the posterior and anterior regions. Almeida et al. ¹² published that the anterior end of medial meniscus was thicker, followed by the posterior and middle regions. The present study is not in agreement with this observation that, we found posterior one-third region of the medial meniscus is thicker than its anterior and middle one-thirds. This supports the clinical findings of Miller ²⁶ that the longitudinal tears of the menisci were more common at the posterior region. The meniscal thickest region is prone to the friction between the condyles of femur and tibia. In the study by using the fetal knee joints, the anterior region of medial meniscus was thicker in comparison to the posterior and middle regions. ²⁴

Miller ²⁶ reported that, middle part of the lateral meniscus is prone to radial tears. Braz and Silva ²³ stated that, middle region of lateral meniscus was thicker, which is trailed by the posterior and anterior regions. Almeida et al. ¹² also described the same observations as Braz and Silva. ²³ The present study observed a different finding that the statistically significant difference was not observed between the thicknesses of the three regions of the lateral menisci. This is similar to the observations of our previous study from fetal specimens. ²⁴

In this anatomical investigation, breadth and thickness measured at the three locations of lateral and medial menisci over the right and left knee joints did not show statistical significance. This confirms the opinion of Dargel et al. ³⁰ that, there is a similarity in the dimensions of the right and left knees, even though they are asymmetrical. Hence, the contralateral radiological measurement of the corresponding meniscus may be considered while choosing the meniscus allograft. Prodromos et al. ³¹ suggested that, during the transplantation surgery, the contralateral meniscal dimensions measured by the magnetic resonance imaging can help in choosing the graft. The present study supports this opinion as there was no statistical significance observed between the comparison of these parameters.

Best meniscal arthroscopic transplant surgeries depend on the correct sizing of the allografts or synthetic material. In this context, the present study has provided morphometric data of the menisci of the lateral and medial compartments in detail with respect to their width and thickness at the anterior, middle and posterior regions. We believe that these data are of help to the arthroscopic surgeon during the comparison of the radiological measurement with the cadaveric measurement and assessing the graft selection.

The ethics committee of our institution approved this research (Ethical Committee Name - Kasturba Medical College and Kasturba Hospital Institutional Ethics Committee, Approval Number - IEC: 284/2021, dated 31.12.2021). Since this is a study from the cadaveric samples, the consent from the participants is not applicable. This was waived by our institutional ethics committee.

Since this is a cross-sectional study from the donated cadavers and did not reveal the identity of the body donor, the written informed consent was not taken from the body donor’s family for the use and publication of this research.