<?xml version="1.0" encoding="UTF-8"?><!DOCTYPE article PUBLIC "-//NLM//DTD JATS (Z39.96) Journal Publishing DTD v1.2 20190208//EN" "http://jats.nlm.nih.gov/publishing/1.2/JATS-journalpublishing1.dtd"><article xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" article-type="brief-report" dtd-version="1.2" xml:lang="en">
    <front>
        <journal-meta>
            <journal-id journal-id-type="pmc">F1000Research</journal-id>
            <journal-title-group>
                <journal-title>F1000Research</journal-title>
            </journal-title-group>
            <issn pub-type="epub">2046-1402</issn>
            <publisher>
                <publisher-name>F1000 Research Limited</publisher-name>
                <publisher-loc>London, UK</publisher-loc>
            </publisher>
        </journal-meta>
        <article-meta>
            <article-id pub-id-type="doi">10.12688/f1000research.73624.1</article-id>
            <article-categories>
                <subj-group subj-group-type="heading">
                    <subject>Brief Report</subject>
                </subj-group>
                <subj-group>
                    <subject>Articles</subject>
                </subj-group>
            </article-categories>
            <title-group>
                <article-title>Hands-on learning of chemistry concepts</article-title>
                <fn-group content-type="pub-status">
                    <fn>
                        <p>[version 1; peer review: 2 approved with reservations, 1 not approved]</p>
                    </fn>
                </fn-group>
            </title-group>
            <contrib-group>
                <contrib contrib-type="author" corresp="yes">
                    <name>
                        <surname>Yew Chi Boon</surname>
                        <given-names>Leonard</given-names>
                    </name>
                    <role content-type="http://credit.niso.org/">Conceptualization</role>
                    <role content-type="http://credit.niso.org/">Formal Analysis</role>
                    <role content-type="http://credit.niso.org/">Investigation</role>
                    <role content-type="http://credit.niso.org/">Methodology</role>
                    <role content-type="http://credit.niso.org/">Writing &#x2013; Original Draft Preparation</role>
                    <uri content-type="orcid">https://orcid.org/0000-0002-6523-6714</uri>
                    <xref ref-type="corresp" rid="c1">a</xref>
                    <xref ref-type="aff" rid="a1">1</xref>
                </contrib>
                <aff id="a1">
                    <label>1</label>Faculty of Information Science and Technology, Multimedia University, Melaka, 75050, Malaysia</aff>
            </contrib-group>
            <author-notes>
                <corresp id="c1">
                    <label>a</label>
                    <email xlink:href="mailto:cbyew@mmu.edu.my">cbyew@mmu.edu.my</email>
                </corresp>
                <fn fn-type="conflict">
                    <p>No competing interests were disclosed.</p>
                </fn>
            </author-notes>
            <pub-date pub-type="epub">
                <day>25</day>
                <month>3</month>
                <year>2022</year>
            </pub-date>
            <pub-date pub-type="collection">
                <year>2022</year>
            </pub-date>
            <volume>11</volume>
            <elocation-id>357</elocation-id>
            <history>
                <date date-type="accepted">
                    <day>9</day>
                    <month>3</month>
                    <year>2022</year>
                </date>
            </history>
            <permissions>
                <copyright-statement>Copyright: &#x00a9; 2022 Yew Chi Boon L</copyright-statement>
                <copyright-year>2022</copyright-year>
                <license xlink:href="https://creativecommons.org/licenses/by/4.0/">
                    <license-p>This is an open access article distributed under the terms of the Creative Commons Attribution Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.</license-p>
                </license>
            </permissions>
            <self-uri content-type="pdf" xlink:href="https://f1000research.com/articles/11-357/pdf"/>
            <abstract>
                <p>
                    <bold>Background</bold> &#x2013; 
                    <italic toggle="yes">S</italic>
                    <italic toggle="yes">toichiometry</italic> is among the most difficult chemistry concepts to teach and it is often taught with an emphasis on 
                    <italic toggle="yes">algorithmic understanding</italic>, without emphasis on 
                    <italic toggle="yes">conceptual understanding.</italic> The purpose of this study was to ascertain if the use of dynamic 
                    <italic toggle="yes">submicro</italic> representations via hands-on learning activities, comprising molecular models and molecular graphics, can provide positive insights into students&#x2019; understanding of 
                    <italic toggle="yes">stoichiometry.</italic>
                </p>
                <p>
                    <bold>Methods</bold> &#x2013; An 
                    <italic toggle="yes">action research</italic> was carried out involving two cycles of lessons i.e., cycle 1 with &#x201c;static&#x201d; 
                    <italic toggle="yes">submicro</italic> representations (PowerPoint) to teach 
                    <italic toggle="yes">stoichiometry</italic> and cycle 2 with &#x201c;dynamic&#x201d; 
                    <italic toggle="yes">submicro</italic> representations (
                    <italic toggle="yes">hands-on</italic> learning with molecular models and graphics) to teach 
                    <italic toggle="yes">stoichiometry.</italic> To gather data, these instruments were used: (1) a 
                    <italic toggle="yes">stoichiometry</italic> test with structured questions, (2) students&#x2019; activity journals and (3) open-ended semi-structured interviews.</p>
                <p>
                    <bold>Results</bold> &#x2013; This study indicated that students who had learnt 
                    <italic toggle="yes">stoichiometry</italic> with only &#x201c;static&#x201d; 
                    <italic toggle="yes">submicro</italic> representations, faced difficulties in understanding 
                    <italic toggle="yes">stoichiometry</italic> at the 
                    <italic toggle="yes">submicro</italic> level. This study also showed that students can benefit from the use of &#x201c;dynamic&#x201d; 
                    <italic toggle="yes">submicro</italic> representations (
                    <italic toggle="yes">hands-on</italic> learning activities) in understanding 
                    <italic toggle="yes">stoichiometry</italic> at the 
                    <italic toggle="yes">submicro </italic>level. Positive changes in students&#x2019; understanding were categorised as a change from a &#x2018;Lack of understanding&#x2019; to &#x2018;Correct understanding&#x2019;. This was determined from transcribed data obtained from the interview sessions and the students&#x2019; activity journals.</p>
                <p>
                    <bold>Conclusion</bold> &#x2013; This study provided evidence that the use of &#x201c;dynamic&#x201d; 
                    <italic toggle="yes">submicro</italic> representations can foster 
                    <italic toggle="yes">conceptual understanding</italic> of 
                    <italic toggle="yes">stoichiometry</italic> at the 
                    <italic toggle="yes">submicro </italic>level. As the local context of the study undertaken by the researcher was the prevailing factor that made this 
                    <italic toggle="yes">action research</italic> unique, the scope of the research and the pedagogical restrictions encountered by the researcher were considered the study&#x2019;s limitations. The uniqueness of this study lies in its approach of involving 
                    <italic toggle="yes">hands-on</italic> learning activities in lessons on 
                    <italic toggle="yes">stoichiometry.</italic>
                </p>
            </abstract>
            <kwd-group kwd-group-type="author">
                <kwd>stoichiometry</kwd>
                <kwd>submicro</kwd>
                <kwd>action research</kwd>
                <kwd>hands-on learning</kwd>
            </kwd-group>
            <funding-group>
                <funding-statement>The author(s) declared that no grants were involved in supporting this work.</funding-statement>
            </funding-group>
        </article-meta>
    </front>
    <body>
        <sec id="sec1" sec-type="intro">
            <title>Introduction</title>
            <p>Chemistry is regarded as a challenging and complex subject (
                <xref ref-type="bibr" rid="ref16">Nakhleh, 1992</xref>; 
                <xref ref-type="bibr" rid="ref10">Gabel, 1999</xref>). This is because chemistry is filled with abstract concepts and deals mostly with 
                <italic toggle="yes">submicro</italic> (particulate) entities that are difficult to represent in the real world (
                <xref ref-type="bibr" rid="ref12">Griffiths &amp; Preston, 1992</xref>). Studies have argued that the imagined world of the 
                <italic toggle="yes">submicro</italic> level is among the most challenging aspects of understanding chemistry (
                <xref ref-type="bibr" rid="ref14">Johnstone, 2000</xref>; 
                <xref ref-type="bibr" rid="ref1">Bucat &amp; Mocerino, 2009</xref>).</p>
            <p>Students&#x2019; issues in understanding 
                <italic toggle="yes">submicro</italic> representations are said to be a source of misunderstanding in chemistry (
                <xref ref-type="bibr" rid="ref19">Tasker &amp; Dalton, 2006</xref>; 
                <xref ref-type="bibr" rid="ref15">Kelly et al., 2010</xref>). Studies have suggested that using particulate (
                <italic toggle="yes">submicro</italic>) drawings (
                <xref ref-type="bibr" rid="ref18">Sanger, 2000</xref>) or 
                <italic toggle="yes">submicro</italic> diagrams (
                <xref ref-type="bibr" rid="ref7">Davidowitz et al., 2010</xref>) encourages 
                <italic toggle="yes">conceptual understanding</italic> at the 
                <italic toggle="yes">submicro</italic> level as these 
                <italic toggle="yes">submicro</italic> drawings or diagrams enable students&#x2019; visualization of abstract chemical concepts such as stoichiometric problems (
                <xref ref-type="bibr" rid="ref7">Davidowitz et al., 2010</xref>).</p>
            <p>This brings us to one of the most important topics in chemistry, 
                <italic toggle="yes">stoichiometry</italic> (
                <xref ref-type="bibr" rid="ref2">Chandrasegaran et al., 2009</xref>; 
                <xref ref-type="bibr" rid="ref7">Davidowitz et al., 2010</xref>). Notably, 
                <italic toggle="yes">stoichiometry</italic> deals with quantitative relationships through the use of chemical formulae and equations and is commonly taught in chemistry courses at the tertiary level (
                <xref ref-type="bibr" rid="ref8">Evans, Yaron &amp; Leinhardt, 2008</xref>). 
                <italic toggle="yes">Stoichiometry</italic> has often been rated to be among the most difficult chemistry topics to teach (
                <xref ref-type="bibr" rid="ref13">Haim, Cort&#x00f3;n, Kocmur &amp; Galagovsky, 2003</xref>). The usual approach to the teaching of 
                <italic toggle="yes">stoichiometry</italic> is through the use of algorithmic strategies with little to no emphasis at all on 
                <italic toggle="yes">conceptual understanding</italic> at the 
                <italic toggle="yes">submicro</italic> level.</p>
            <p>Studies have indicated that many students do not achieve an adequate understanding of s
                <italic toggle="yes">toichiometry</italic>-related concepts despite formal education (
                <xref ref-type="bibr" rid="ref11">Gauchon &amp; M&#x00e9;heut, 2007</xref>; 
                <xref ref-type="bibr" rid="ref6">Dahsah &amp; Coll, 2008</xref>; 
                <xref ref-type="bibr" rid="ref2">Chandrasegaran et al., 2009</xref>). A notable problem inherent to the learning of 
                <italic toggle="yes">stoichiometry</italic> is the tendency of students to solve 
                <italic toggle="yes">stoichiometry</italic> problems in an algorithmic manner without 
                <italic toggle="yes">conceptual understanding</italic> (
                <xref ref-type="bibr" rid="ref17">Salta &amp; Tzougraki, 2010</xref>).</p>
            <p>The teaching and learning of 
                <italic toggle="yes">stoichiometry</italic> does not include the use of concrete 
                <italic toggle="yes">submicro</italic> models at all during classroom instruction. Instead, only &#x201c;static&#x201d; 
                <italic toggle="yes">submicro</italic> diagrams (in PowerPoint slides) are used in conventional classrooms in the teaching of 
                <italic toggle="yes">stoichiometry.</italic>
            </p>
            <p>The main problem with students&#x2019; lack of 
                <italic toggle="yes">conceptual understanding</italic> of 
                <italic toggle="yes">stoichiometry</italic> is directly linked to students&#x2019; lack of understanding of the topic itself at the 
                <italic toggle="yes">submicro</italic> level (
                <xref ref-type="bibr" rid="ref7">Davidowitz et al., 2010</xref>). This shortcoming in the teaching and learning of 
                <italic toggle="yes">stoichiometry</italic> at the 
                <italic toggle="yes">submicro</italic> level in the researcher&#x2019;s own classroom is the basis for this study.</p>
        </sec>
        <sec id="sec2" sec-type="methods">
            <title>Methods</title>
            <p>An 
                <italic toggle="yes">action research</italic> is carried out to study a specific problem (
                <xref ref-type="bibr" rid="ref9">Fraenkel &amp; Wallen, 2007</xref>). For this study, an 
                <italic toggle="yes">action research</italic> was carried out to shed light on the issue, i.e., addressing students&#x2019; lack of 
                <italic toggle="yes">conceptual</italic> understanding of 
                <italic toggle="yes">stoichiometry</italic> at the 
                <italic toggle="yes">submicro</italic> level. To do so, two cycles of lessons on 
                <italic toggle="yes">stoichiometry</italic> were carried out, i.e., cycle 1 with &#x201c;static&#x201d; 
                <italic toggle="yes">submicro</italic> representations (PowerPoint slides) to teach 
                <italic toggle="yes">stoichiometry</italic> and cycle 2 with &#x201c;dynamic&#x201d; 
                <italic toggle="yes">submicro</italic> representations. The &#x201c;dynamic&#x201d; 
                <italic toggle="yes">submicro</italic> representations included (1) &#x201c;dynamic&#x201d; 
                <italic toggle="yes">submicro</italic> diagrams (molecular graphics manipulated with the computer - 
                <italic toggle="yes">hands-on</italic> approach) and (2) 
                <italic toggle="yes">submicro</italic> models (physical molecular models manipulated by hand - 
                <italic toggle="yes">hands-on</italic> approach).</p>
            <p>&#x201c;Dynamic&#x201d; 
                <italic toggle="yes">submicro</italic> representations functioned as supplementary methods to teach 
                <italic toggle="yes">stoichiometry</italic> in addition to conventional classroom approaches (with only &#x201c;static&#x201d; 
                <italic toggle="yes">submicro</italic> representations). The use of &#x201c;dynamic&#x201d; 
                <italic toggle="yes">submicro</italic> representations allowed students to learn 
                <italic toggle="yes">stoichiometry</italic> in a 
                <italic toggle="yes">hands-on</italic> manner by playing with molecular graphics via the computer or by manipulating 
                <italic toggle="yes">submicro</italic> models with their hands. The use of &#x201c;dynamic&#x201d; 
                <italic toggle="yes">submicro</italic> representations allowed students to learn in an active manner rather than a passive manner.</p>
            <p>The participants in this study were a cohort of pre-university (grade 12) students who were the researcher&#x2019;s own students comprising eight students. This was an example of convenience sampling. These students were the only students in the researcher&#x2019;s general chemistry class. The students were first briefed about the study in a specially conducted session during class (1
                <sup>st</sup> week of the trimester) and once they were aware that the study would complement the course itself, all these eight students collectively volunteered to be part of the study and fully consented to the manner in which the general chemistry course as well as the research would be carried out as explained by the researcher. Full verbal consent was obtained from all eight students with the assurance that their identities and privacies would be protected. Oral consent received in this research was reported to the University&#x2019;s Research Ethics Committee (REC) and an approval number was then granted. All in all, the students were aware of the purpose of the study, their important role in the study as participants and that their learning activities would constitute part of the curriculum for the subject involved. In this 
                <italic toggle="yes">action research</italic>, qualitative data were collected from (1) students&#x2019; answers to structured stoichiometric questions in a 
                <italic toggle="yes">stoichiometry</italic> test (included 
                <italic toggle="yes">submicro</italic> drawings drawn by the students themselves), (2) individual interview sessions and (3) students&#x2019; entries into their journals. Together, these three sources of qualitative data formed the overall data collection techniques in this 
                <italic toggle="yes">action research</italic> (
                <xref ref-type="table" rid="T1">Table 1</xref>).</p>
            <table-wrap id="T1" orientation="portrait" position="float">
                <label>Table 1. </label>
                <caption>
                    <title>Data collection techniques.</title>
                </caption>
                <table content-type="article-table" frame="hsides">
                    <thead>
                        <tr>
                            <th align="left" colspan="1" rowspan="1" valign="top">Technique 1</th>
                            <th align="left" colspan="1" rowspan="1" valign="top">Technique 2</th>
                            <th align="left" colspan="1" rowspan="1" valign="top">Technique 3</th>
                        </tr>
                    </thead>
                    <tbody>
                        <tr>
                            <td align="center" colspan="1" rowspan="1" valign="middle">Stoichiometry test</td>
                            <td align="center" colspan="1" rowspan="1" valign="middle">Individual interview session</td>
                            <td align="center" colspan="1" rowspan="1" valign="middle">Students&#x2019; journal</td>
                        </tr>
                    </tbody>
                </table>
            </table-wrap>
            <p>The 
                <italic toggle="yes">stoichiometry</italic> test comprised of eight structured questions for pre-university level chemistry on 
                <italic toggle="yes">stoichiometry</italic> with emphasis on understanding at the 
                <italic toggle="yes">submicro</italic> level. These questions were either developed with modifications from two Chemistry textbooks or were loosely based on questions used by previous researchers. The 
                <italic toggle="yes">stoichiometry</italic> test contained different formats of questioning whereby students were required to either interpret 
                <italic toggle="yes">submicro</italic> diagrams or relate 
                <italic toggle="yes">submicro</italic> diagrams to the symbolic level or even produce their own 
                <italic toggle="yes">submicro</italic> drawings.</p>
            <p>The interview sessions were solely conducted by the researcher himself who had seven years of working experience teaching science subjects and was pursuing a Masters in Science Education at the time of the study. It must be noted that the researcher was also the only lecturer teaching general chemistry to these eight students. The interview sessions were carried out each time after the application of the 
                <italic toggle="yes">stoichiometry</italic> test to probe students&#x2019; answers in their 
                <italic toggle="yes">stoichiometry</italic> test. The interview sessions which were audio recorded, were open-ended and semi-structured and this allowed the researcher to probe the students&#x2019; 
                <italic toggle="yes">conceptual understanding</italic> of 
                <italic toggle="yes">stoichiometry</italic> at the 
                <italic toggle="yes">submicro</italic> level.</p>
            <p>As another source of data, students were also asked to record their thoughts in their journals in cycle 2 during the additional lessons phase in which &#x201c;dynamic&#x201d; 
                <italic toggle="yes">submicro</italic> representations were used. The students&#x2019; entries into their activity journals (cycle 2 only) were meant to gather additional data and to triangulate all the sources of data. While the journal entries were written by the students after lessons with the &#x201c;dynamic&#x201d; 
                <italic toggle="yes">submicro</italic> representations, these entries were based on guided questions.</p>
            <p>The research flow for this 
                <italic toggle="yes">action research</italic> consisted of two cycles. In cycle 1, lessons on 
                <italic toggle="yes">stoichiometry</italic> were conducted with &#x201c;static&#x201d; 
                <italic toggle="yes">submicro</italic> representations while in cycle 2, lessons on 
                <italic toggle="yes">stoichiometry</italic> were conducted with &#x201c;dynamic&#x201d; 
                <italic toggle="yes">submicro</italic> representations. After lessons with &#x201c;static&#x201d; 
                <italic toggle="yes">submicro</italic> representations in cycle 1, students attempted 
                <italic toggle="yes">stoichiometry</italic> test 1.</p>
            <p>Interview session 1 was then carried out. Analysis of the qualitative data and reflection by the researcher followed these steps at the end of cycle 1. Upon completion of cycle 1, cycle 2 began with chemistry lessons on 
                <italic toggle="yes">stoichiometry</italic> with &#x201c;dynamic&#x201d; 
                <italic toggle="yes">submicro</italic> representations.</p>
            <p>As students had to use 
                <italic toggle="yes">submicro</italic> models followed by 
                <italic toggle="yes">molecular graphics</italic> as part of the use of &#x201c;dynamic&#x201d; 
                <italic toggle="yes">submicro</italic> representations in cycle 2, they were asked to fill in their activity journals after each of the two approaches mentioned. Once this had been completed, students attempted 
                <italic toggle="yes">stoichiometry</italic> test 2. Similar to cycle 1, interview session 2 was then carried out in cycle 2.</p>
            <p>Analysis of the qualitative data and reflection by the researcher was carried out again in cycle 2 just as it was done so in cycle 1. The sequence of steps mentioned in both cycle 1 and cycle 2 correspond to the major steps of an 
                <italic toggle="yes">action research</italic>, namely &#x201c;plan&#x201d; (step 1) followed by &#x201c;act and observe&#x201d; (step 2) and followed by &#x201c;reflect&#x201d; (step 3). This was evident in cycle 1 and cycle 2 of this 
                <italic toggle="yes">action research.</italic>
            </p>
        </sec>
        <sec id="sec3" sec-type="results">
            <title>Results</title>
            <p>Students&#x2019; responses to the questions and their drawings of 
                <italic toggle="yes">submicro</italic> representations in the tests were analysed based on a 
                <italic toggle="yes">dichotomous</italic> scale (&#x2018;Correct understanding&#x2019; and a &#x2018;Lack of understanding&#x2019;) manually without the use of any software. These responses&#x00a0;were used to determine whether a change in students&#x2019; understanding had occurred between cycle 1 and cycle 2. &#x2018;Correct understanding&#x2019; means that the question had been answered correctly without mistakes while &#x2018;Lack of understanding&#x2019; means that the question had been answered incorrectly with mistakes. An example is provided to illustrate this dichotomy.</p>
            <p>For example, in question 2 of the test, students drew the correct number of molecules (as 
                <italic toggle="yes">submicro</italic> representations) for all reactants and products represented by the balanced chemical equation in the question (involves self-generated 
                <italic toggle="yes">submicro</italic> drawings). In this given example (
                <xref ref-type="fig" rid="f1">Figure 1</xref>), the student correctly drew two molecules of C
                <sub>2</sub>H
                <sub>6</sub> which react with seven molecules of O
                <sub>2</sub> in the box for &#x2018;before reaction&#x2019; and drew four CO
                <sub>2</sub> molecules and six H
                <sub>2</sub>O molecules in the &#x2018;after reaction&#x2019; box. Such an answer was classified as &#x2018;Correct understanding&#x2019;. Answers which deviated from this were classified as &#x2018;Lack of understanding&#x2019;.</p>
            <fig fig-type="figure" id="f1" orientation="portrait" position="float">
                <label>Figure 1. </label>
                <caption>
                    <title>Example of &#x2018;Correct understanding&#x2019;.</title>
                </caption>
                <graphic id="gr1" orientation="portrait" position="float" xlink:href="https://f1000research-files.f1000.com/manuscripts/77288/3b37a9d1-25c0-45d8-b84c-b42585f2de31_figure1.gif"/>
            </fig>
            <p>For the eight questions in the 
                <italic toggle="yes">stoichiometry</italic> tests, questions 2-8 were related to students&#x2019; 
                <italic toggle="yes">conceptual understanding</italic> at the 
                <italic toggle="yes">submicro</italic> level. 
                <xref ref-type="table" rid="T2">Table 2a</xref> (questions 2-4) and 
                <xref ref-type="table" rid="T3">Table 2b</xref> (questions 5-8) are summaries of the results of the 
                <italic toggle="yes">stoichiometry</italic> tests.</p>
            <table-wrap id="T2" orientation="portrait" position="float">
                <label>Table 2a. </label>
                <caption>
                    <title>Students&#x2019; understanding: questions 2, 3 &amp; 4.</title>
                </caption>
                <table content-type="article-table" frame="hsides">
                    <thead>
                        <tr>
                            <th align="left" colspan="1" rowspan="1" valign="top"/>
                            <th align="left" colspan="1" rowspan="1" valign="top">Stoichiometry test 1</th>
                            <th align="left" colspan="1" rowspan="1" valign="top">Stoichiometry test 2</th>
                        </tr>
                    </thead>
                    <tbody>
                        <tr>
                            <td align="center" colspan="1" rowspan="1" valign="middle">2</td>
                            <td align="left" colspan="1" rowspan="1" valign="middle">Correct understanding in students 2, 3, 4, 7 &amp; 8
                                <break/>Lack of understanding in students 1, 5 &amp; 6</td>
                            <td align="left" colspan="1" rowspan="1" valign="middle">Correct understanding in all 8 students</td>
                        </tr>
                        <tr>
                            <td align="center" colspan="1" rowspan="1" valign="middle">3</td>
                            <td align="left" colspan="1" rowspan="1" valign="middle">Correct understanding in students 2 &amp; 4
                                <break/>Lack of understanding in students 1, 3, 5, 6, 7 &amp; 8</td>
                            <td align="left" colspan="1" rowspan="1" valign="middle">Correct understanding in all 8 students</td>
                        </tr>
                        <tr>
                            <td align="center" colspan="1" rowspan="1" valign="middle">4</td>
                            <td align="left" colspan="1" rowspan="1" valign="middle">Correct understanding in students 2, 4 &amp; 7
                                <break/>Lack of understanding in students 1, 3, 5, 6 &amp; 8</td>
                            <td align="left" colspan="1" rowspan="1" valign="middle">Correct understanding in all 8 students</td>
                        </tr>
                    </tbody>
                </table>
            </table-wrap>
            <table-wrap id="T3" orientation="portrait" position="float">
                <label>Table 2b. </label>
                <caption>
                    <title>Students&#x2019; understanding: questions 5, 6, 7 &amp; 8.</title>
                </caption>
                <table content-type="article-table" frame="hsides">
                    <thead>
                        <tr>
                            <th align="left" colspan="1" rowspan="1" valign="top"/>
                            <th align="left" colspan="1" rowspan="1" valign="top">Stoichiometry test 1</th>
                            <th align="left" colspan="1" rowspan="1" valign="top">Stoichiometry test 2</th>
                        </tr>
                    </thead>
                    <tbody>
                        <tr>
                            <td align="center" colspan="1" rowspan="1" valign="middle">5</td>
                            <td align="left" colspan="1" rowspan="1" valign="middle">Correct understanding in students 3, 5, 6 &amp; 7
                                <break/>Lack of understanding in students 1, 2, 4 &amp; 8</td>
                            <td align="left" colspan="1" rowspan="1" valign="middle">Correct understanding in students 3, 4, 5, 6 &amp; 7
                                <break/>Lack of understanding in students 1, 2 &amp; 8</td>
                        </tr>
                        <tr>
                            <td align="center" colspan="1" rowspan="1" valign="middle">6</td>
                            <td align="left" colspan="1" rowspan="1" valign="middle">Correct understanding in students 3, 7 &amp; 8
                                <break/>Lack of understanding in students 1, 2, 4, 5 &amp; 6</td>
                            <td align="left" colspan="1" rowspan="1" valign="middle">Correct understanding in students 3, 4, 5, 6 &amp; 7
                                <break/>Lack of understanding in students 1, 2 &amp; 8</td>
                        </tr>
                        <tr>
                            <td align="center" colspan="1" rowspan="1" valign="middle">7</td>
                            <td align="left" colspan="1" rowspan="1" valign="middle">Correct understanding in students 4 &amp; 7
                                <break/>Lack of understanding in students 1, 2, 3, 5, 6 &amp; 8</td>
                            <td align="left" colspan="1" rowspan="1" valign="middle">Correct understanding in students 1, 2, 4, 5, 6, 7 &amp; 8
                                <break/>Lack of understanding in student 3</td>
                        </tr>
                        <tr>
                            <td align="center" colspan="1" rowspan="1" valign="middle">8</td>
                            <td align="left" colspan="1" rowspan="1" valign="middle">Correct understanding in students 2, 4 &amp; 7
                                <break/>Lack of understanding in students 1, 3, 5, 6 &amp; 8</td>
                            <td align="left" colspan="1" rowspan="1" valign="middle">Correct understanding in students 1, 2, 5, 6, 7 &amp; 8
                                <break/>Lack of understanding in students 3 &amp; 4</td>
                        </tr>
                    </tbody>
                </table>
            </table-wrap>
            <p>As observed in 
                <xref ref-type="table" rid="T2">Table 2a</xref> and 
                <xref ref-type="table" rid="T3">Table 2b</xref>, there were noticeable improvements in some students&#x2019; understanding for questions 2-8 when a comparison was made between 
                <italic toggle="yes">stoichiometry</italic> test 1 and test 2. Questions 2, 3 and 4 required students to self-generate 
                <italic toggle="yes">submicro</italic> drawings and positive improvements were observed for many students. For questions 5 and 6, students interpreted 
                <italic toggle="yes">submicro</italic> diagrams. As seen in 
                <xref ref-type="table" rid="T3">Table 2b</xref>, some students still demonstrated a &#x2018;Lack of understanding&#x2019; for these two questions in test 2.</p>
            <p>For questions 7 and 8, students had to interpret 
                <italic toggle="yes">submicro</italic> diagrams as well as self-generate 
                <italic toggle="yes">submicro</italic> drawings. The comparison between 
                <italic toggle="yes">stoichiometry</italic> test 1 and test 2 for these two questions indicated that only a handful of students still demonstrated a &#x2018;Lack of understanding&#x2019; for a few questions in 
                <italic toggle="yes">stoichiometry</italic> test 2. Overall, positive improvements can be observed in the participants&#x2019; answers in 
                <italic toggle="yes">stoichiometry</italic> test 2 as compared to test 1.</p>
            <p>To provide insights into the factors that may be able to explain the findings of this study, the qualitative data from the interview sessions and the students&#x2019; journal entries were analysed to provide some answers. To this end, a coding framework was used in analyzing the transcribed data from these two sources of data. From the analysis of the transcribed text from these sources of data, &#x2018;codes&#x2019; were formed. In the next step of the analysis, the variety of &#x2018;codes&#x2019; was grouped together to form &#x2018;categories&#x2019; which included the grouping of related &#x2018;codes&#x2019; in each bigger &#x2018;category&#x2019;. From &#x2018;categories&#x2019; which can be collapsed, &#x2018;themes&#x2019; were subsequently generated. This was done repeatedly over several rounds or cycles of grouping and categorising until the point of saturation was reached, in which a clear &#x2018;theme&#x2019; can be discerned and defined.</p>
            <p>As this study was carried out as an 
                <italic toggle="yes">action research</italic>, building themes to provide answers will be more important than answering hypotheses (
                <xref ref-type="bibr" rid="ref3">Checkland &amp; Holwell, 1998</xref>) and thus, this theme-building approach played the central role in interpreting the data collected in this 
                <italic toggle="yes">action research.</italic> 
                <xref ref-type="bibr" rid="ref5">Creswell (2008)</xref> wrote that themes are aggregated codes with similarities. Standout themes are important research elements that can provide answers in the analysis of the type of qualitative data collected in this study. As shown in 
                <xref ref-type="table" rid="T4">Table 3</xref> below, two prominent themes were evidently detected via the process described above.</p>
            <table-wrap id="T4" orientation="portrait" position="float">
                <label>Table 3. </label>
                <caption>
                    <title>Transcribed data as &#x2018;themes&#x2019;.</title>
                </caption>
                <table content-type="article-table" frame="hsides">
                    <thead>
                        <tr>
                            <th align="left" colspan="1" rowspan="1" valign="top">Theme: &#x2018;Understanding based on touch or play&#x2019;</th>
                            <th align="left" colspan="1" rowspan="1" valign="top">Theme: &#x2018;Understanding based on imagination&#x2019;</th>
                        </tr>
                    </thead>
                    <tbody>
                        <tr>
                            <td align="left" colspan="1" rowspan="1" valign="top">
                                <styled-content style="#0D0D0D" style-type="color">&#x201c;
                                    <italic toggle="yes">The whole experience was like playing with Lego. It was interesting because with the molecular models, we can understand better what is happening in a reaction from a molecular point of view</italic>&#x201d;. (Student 2, 
                                    <italic toggle="yes">journal</italic>)</styled-content>
                                <break/>
                                <styled-content style="#0D0D0D" style-type="color">&#x201c;&#x2026; 
                                    <italic toggle="yes">important factors that making the experience enjoyable was able to mix difference colour or shape to create own molecular structure. Through this, I understand more the concept for limiting and excess reagent</italic>&#x201d;. (Student 3, 
                                    <italic toggle="yes">journal</italic>)</styled-content>
                                <break/>
                                <styled-content style="#0D0D0D" style-type="color">&#x201c;
                                    <italic toggle="yes">After playing with the models, and actually touching it, touching the models physically, in thinking, I can come up with a correct conclusion by just looking at the diagram</italic>&#x201d;. (Student 7, 
                                    <italic toggle="yes">interview</italic>)</styled-content>
                                <break/>
                                <styled-content style="#0D0D0D" style-type="color">&#x201c;
                                    <italic toggle="yes">So having these model sessions and PowerPoint slides we actually learn how to visualize and we can touch it physically. So, it basically improves our understanding even more</italic>&#x201d;. (Student 7, 
                                    <italic toggle="yes">interview</italic>)</styled-content>
                            </td>
                            <td align="left" colspan="1" rowspan="1" valign="top">
                                <styled-content style="#0D0D0D" style-type="color">&#x201c;
                                    <italic toggle="yes">Now, I can have a mental image of molecules breaking down to form other molecules whenever I see an equation</italic>&#x201d;. (Student 8, 
                                    <italic toggle="yes">journal</italic>)</styled-content>
                                <break/>
                                <styled-content style="#0D0D0D" style-type="color">&#x201c;
                                    <italic toggle="yes">When I am doing the question, I can imagine the models, those atoms and the diagrams in my head &#x2026; So that&#x2019;s why I come up with the conclusion faster</italic>&#x201d;. (Student 7, 
                                    <italic toggle="yes">journal</italic>)</styled-content>
                                <break/>
                                <styled-content style="#0D0D0D" style-type="color">&#x201c;
                                    <italic toggle="yes">I can imagine the thing in my head. And I can know directly which is the limiting reagent, which is the excess factor and I can be sure in my answer with that</italic>&#x201d;. (Student 7, 
                                    <italic toggle="yes">interview</italic>)</styled-content>
                                <break/>
                                <styled-content style="#0D0D0D" style-type="color">&#x201c;
                                    <italic toggle="yes">I think of the models because as I stated before the models give me the mental image. It can allow me to imagine the things, how they form &#x2026;</italic>&#x201d; (Student 8, 
                                    <italic toggle="yes">interview</italic>)</styled-content>
                            </td>
                        </tr>
                    </tbody>
                </table>
            </table-wrap>
            <p>As shown in 
                <xref ref-type="table" rid="T4">Table 3</xref>, two prominent &#x2018;themes&#x2019; were generated in this study, i.e., &#x2018;understanding based on touch or play&#x2019; and &#x2018;understanding based on imagination&#x2019;. These &#x2018;themes&#x2019; were generated from the transcribed interview data and the students&#x2019; journal entries. These sources of data provided contextual clues and generated plausible explanations for the improvements seen in students&#x2019; 
                <italic toggle="yes">conceptual understanding</italic> of 
                <italic toggle="yes">stoichiometry</italic> at the 
                <italic toggle="yes">submicro</italic> level. Note: only a small portion of the actual data is shown in this article due to the word-count limitation.</p>
        </sec>
        <sec id="sec4" sec-type="discussion">
            <title>Discussion</title>
            <p>For the theme &#x2018;understanding based on touch or play&#x2019;, it is noteworthy that the use of 
                <italic toggle="yes">submicro</italic> models can account for the changes in students&#x2019; 
                <italic toggle="yes">conceptual understanding</italic> of 
                <italic toggle="yes">stoichiometry.</italic> It was not just the visuals of the models which provided students with improved 
                <italic toggle="yes">conceptual understanding</italic> at the 
                <italic toggle="yes">submicro</italic> level; it was also the manipulation of the models which had fostered understanding through &#x2018;touch&#x2019; (
                <italic toggle="yes">hands-on</italic>).</p>
            <p>Consider this illuminating interview excerpt:</p>
            <disp-quote>
                <p>&#x201c;
                    <italic toggle="yes">.&#x2026; after playing with the models and actually touching it, touching the models physically, in thinking I can come up with a correct conclusion by just looking at the diagram.</italic>&#x201d;</p>
            </disp-quote>
            <p>Based on the theme &#x2018;understanding based on imagination&#x2019;, it is fair to note that the manipulation of the 
                <italic toggle="yes">submicro</italic> models left a discernible impact on the students&#x2019; 
                <italic toggle="yes">conceptual understanding</italic> of 
                <italic toggle="yes">stoichiometry.</italic>
            </p>
            <p>Consider this revealing interview excerpt:</p>
            <disp-quote>
                <p>&#x201c;
                    <italic toggle="yes">I can imagine the thing [molecules] in my head and I can know directly which is the limiting reagent, which is the excess factor and I can be sure in my answer with that.</italic>&#x201d;</p>
            </disp-quote>
            <p>From the interview and journal transcripts obtained in this 
                <italic toggle="yes">action research</italic>, the researcher was able to find contextual clues that shed light on the usefulness of &#x201c;dynamic&#x201d; 
                <italic toggle="yes">submicro</italic> representations to help students understand 
                <italic toggle="yes">stoichiometry.</italic> It suffices to say that the findings of this study are relevant to researchers studying chemistry education. This comes as researchers have long stressed that the visual mode of representation in chemistry education is under-explored (
                <xref ref-type="bibr" rid="ref4">Cheng &amp; Gilbert, 2009</xref>) while 
                <xref ref-type="bibr" rid="ref2">Chandrasegaran et al. (2009)</xref> had recommended that instructors try a variety of approaches including an emphasis on the changes which occur at the 
                <italic toggle="yes">submicro</italic> level to enhance students&#x2019; 
                <italic toggle="yes">conceptual understanding.</italic>
            </p>
        </sec>
        <sec id="sec5" sec-type="conclusion">
            <title>Conclusion</title>
            <p>As this 
                <italic toggle="yes">action research</italic> focused on only eight students and was constrained by the local context of the study (e.g., the pedagogical restrictions, etc), the findings of this study cannot be generalized and this is its primary limitation. Nonetheless, even with the small number of students, the results of this study provide evidence to the researcher&#x2019;s assertion that &#x201c;dynamic&#x201d; 
                <italic toggle="yes">submicro</italic> representations can foster 
                <italic toggle="yes">conceptual understanding</italic> of 
                <italic toggle="yes">stoichiometry</italic> at the 
                <italic toggle="yes">submicro</italic> level. The main contribution of this 
                <italic toggle="yes">action research</italic> is that it highlights the importance of focusing on the 
                <italic toggle="yes">submicro</italic> level of understanding to achieve 
                <italic toggle="yes">conceptual understanding</italic> in chemistry and provides an example of how this can be done via the use of a 
                <italic toggle="yes">hands-on</italic> learning approach to teaching chemistry concepts such as 
                <italic toggle="yes">stoichiometry.</italic>
            </p>
        </sec>
        <sec id="sec6">
            <title>Author contribution</title>
            <p>The main author is the only author that had contributed to the writing of this article based on an 
                <italic toggle="yes">action research.</italic>
            </p>
        </sec>
        <sec id="sec7">
            <title>Consent</title>
            <p>The author had obtained full, unequivocal verbal consent from all the eight participants in this research who were students who had enrolled for the chemistry course. The researcher had taught the chemistry course in the trimester in which the 
                <italic toggle="yes">action research</italic> was conducted.</p>
        </sec>
        <sec id="sec8">
            <title>Ethical Approval (EA) Number</title>
            <p>Approval number: EA2082021 was granted by Research Ethics Committee (REC) of Technology Transfer Office (TTO).</p>
        </sec>
        <sec id="sec9">
            <title>Dataset</title>
            <p>Dans Easy: Hands-On Learning of Chemistry Concepts. 
                <ext-link ext-link-type="uri" xlink:href="https://doi.org/10.17026/dans-2ce-u2j8">https://doi.org/10.17026/dans-2ce-u2j8</ext-link> (
                <xref ref-type="bibr" rid="ref20">Yew, 2021</xref>)</p>
            <p>This project contains the following underlying data.
                <list list-type="bullet">
                    <list-item>
                        <label>&#x2022;</label>
                        <p>Appendices (A collection of materials used in the research &#x2013; Appendices A-M)</p>
                    </list-item>
                    <list-item>
                        <label>&#x2022;</label>
                        <p>01-Stoichiometry Test.pdf (Appendix A: Stoichiometry test consisting of 8 questions)</p>
                    </list-item>
                    <list-item>
                        <label>&#x2022;</label>
                        <p>02-Interview-Protocol.pdf (Appendix C: The interview protocol)</p>
                    </list-item>
                    <list-item>
                        <label>&#x2022;</label>
                        <p>03-Theme Formation.pdf&#x00a0;(Draft of the Table used to categorise Theme Formation)</p>
                    </list-item>
                    <list-item>
                        <label>&#x2022;</label>
                        <p>Data Set for Dans Easy Leonard.pdf (Description of the research: research methodology, findings with examples of students&#x2019; answers and a description of all the appendixes)</p>
                    </list-item>
                </list>
            </p>
            <p>Data are available under the terms of the 
                <ext-link ext-link-type="uri" xlink:href="http://creativecommons.org/publicdomain/zero/1.0/">Creative Commons Zero &#x201c;No rights reserved&#x201d; data waiver</ext-link> (CC0 1.0 Public domain dedication).</p>
        </sec>
    </body>
    <back>
        <ack>
            <title>Acknowledgments</title>
            <p>The author(s) would like to acknowledge that this study was carried out and written solely by the main author himself. However, the conceptualisation of the study, the data collection and the analysis of the data were carried out with support, advice and encouragement from Professor Dr. Esther Daniel.</p>
        </ack>
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    </back>
    <sub-article article-type="reviewer-report" id="report152762">
        <front-stub>
            <article-id pub-id-type="doi">10.5256/f1000research.77288.r152762</article-id>
            <title-group>
                <article-title>Reviewer response for version 1</article-title>
            </title-group>
            <contrib-group>
                <contrib contrib-type="author">
                    <name>
                        <surname>Sibanda</surname>
                        <given-names>Doras</given-names>
                    </name>
                    <xref ref-type="aff" rid="r152762a1">1</xref>
                    <role>Referee</role>
                    <uri content-type="orcid">https://orcid.org/0000-0001-9328-3473</uri>
                </contrib>
                <aff id="r152762a1">
                    <label>1</label>University of KwaZulu-Natal, Pietermaritzburg, South Africa</aff>
            </contrib-group>
            <author-notes>
                <fn fn-type="conflict">
                    <p>
                        <bold>Competing interests: </bold>No competing interests were disclosed.</p>
                </fn>
            </author-notes>
            <pub-date pub-type="epub">
                <day>21</day>
                <month>10</month>
                <year>2022</year>
            </pub-date>
            <permissions>
                <copyright-statement>Copyright: &#x00a9; 2022 Sibanda D</copyright-statement>
                <copyright-year>2022</copyright-year>
                <license xlink:href="https://creativecommons.org/licenses/by/4.0/">
                    <license-p>This is an open access peer review report distributed under the terms of the Creative Commons Attribution Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.</license-p>
                </license>
            </permissions>
            <related-article ext-link-type="doi" id="relatedArticleReport152762" related-article-type="peer-reviewed-article" xlink:href="10.12688/f1000research.73624.1"/>
            <custom-meta-group>
                <custom-meta>
                    <meta-name>recommendation</meta-name>
                    <meta-value>approve-with-reservations</meta-value>
                </custom-meta>
            </custom-meta-group>
        </front-stub>
        <body>
            <p>The article addresses an important aspect of the teaching of stoichiometry. The titles should be about Hands-on learning of stoichiometry.</p>
            <p> </p>
            <p> There is need to strengthen the argument in the literature review to show that limited studies have focused on hands on in stoichiometry.&#x00a0;</p>
            <p> </p>
            <p> The methods section was detailed.&#x00a0;</p>
            <p> </p>
            <p> The results section can be improved, for example the information Table 2a can include some examples of what correct understanding means. As it is, the table is not self-contained. The discussion section can be revised with a focus on the key findings of the study. The conclusion should try and provide an answer to the research question.</p>
            <p>Is the work clearly and accurately presented and does it cite the current literature?</p>
            <p>Partly</p>
            <p>If applicable, is the statistical analysis and its interpretation appropriate?</p>
            <p>Not applicable</p>
            <p>Are all the source data underlying the results available to ensure full reproducibility?</p>
            <p>No source data required</p>
            <p>Is the study design appropriate and is the work technically sound?</p>
            <p>Partly</p>
            <p>Are the conclusions drawn adequately supported by the results?</p>
            <p>Partly</p>
            <p>Are sufficient details of methods and analysis provided to allow replication by others?</p>
            <p>Yes</p>
            <p>Reviewer Expertise:</p>
            <p>Chemistry education, conceptual understanding of chemistry concepts, teaching sequences, inqury based science teaching</p>
            <p>I confirm that I have read this submission and believe that I have an appropriate level of expertise to confirm that it is of an acceptable scientific standard, however I have significant reservations, as outlined above.</p>
        </body>
    </sub-article>
    <sub-article article-type="reviewer-report" id="report152759">
        <front-stub>
            <article-id pub-id-type="doi">10.5256/f1000research.77288.r152759</article-id>
            <title-group>
                <article-title>Reviewer response for version 1</article-title>
            </title-group>
            <contrib-group>
                <contrib contrib-type="author">
                    <name>
                        <surname>Saribas</surname>
                        <given-names>Deniz</given-names>
                    </name>
                    <xref ref-type="aff" rid="r152759a1">1</xref>
                    <role>Referee</role>
                    <uri content-type="orcid">https://orcid.org/0000-0002-4839-7858</uri>
                </contrib>
                <aff id="r152759a1">
                    <label>1</label>Istanbul Aydin University, Istanbul, Turkey</aff>
            </contrib-group>
            <author-notes>
                <fn fn-type="conflict">
                    <p>
                        <bold>Competing interests: </bold>No competing interests were disclosed.</p>
                </fn>
            </author-notes>
            <pub-date pub-type="epub">
                <day>21</day>
                <month>10</month>
                <year>2022</year>
            </pub-date>
            <permissions>
                <copyright-statement>Copyright: &#x00a9; 2022 Saribas D</copyright-statement>
                <copyright-year>2022</copyright-year>
                <license xlink:href="https://creativecommons.org/licenses/by/4.0/">
                    <license-p>This is an open access peer review report distributed under the terms of the Creative Commons Attribution Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.</license-p>
                </license>
            </permissions>
            <related-article ext-link-type="doi" id="relatedArticleReport152759" related-article-type="peer-reviewed-article" xlink:href="10.12688/f1000research.73624.1"/>
            <custom-meta-group>
                <custom-meta>
                    <meta-name>recommendation</meta-name>
                    <meta-value>approve-with-reservations</meta-value>
                </custom-meta>
            </custom-meta-group>
        </front-stub>
        <body>
            <p>The paper needs the following revisions: 
                <list list-type="order">
                    <list-item>
                        <p>A brief definition and explanation and what is meant by&#x00a0;
                            <italic>conceptual understanding</italic>&#x00a0;at the&#x00a0;
                            <italic>submicro</italic>&#x00a0;level should be given in the introduction section.</p>
                    </list-item>
                    <list-item>
                        <p>The literature cited throughout the paper is outdated. More recent and up-to-date research studies should be cited both in the introduction and the discussion sections.</p>
                    </list-item>
                    <list-item>
                        <p>It should be clear why this research is an action research. Which features of this study fits to action research design? This question should be addressed.</p>
                    </list-item>
                    <list-item>
                        <p>Data collection procedure should be elaborated. What kind of questions do the test and interview questions involve and which thoughts were the participants asked to reflect on their journals should be explained. The researcher's reflection is also unclear. What did the researcher reflect on?</p>
                    </list-item>
                    <list-item>
                        <p>The procedures to ensure the validity and reliability of the study should be explained.</p>
                    </list-item>
                    <list-item>
                        <p>In table 3, the themes&#x00a0;&#x2018;understanding based on touch or play&#x2019; and &#x2018;understanding based on imagination&#x2019; should be defined. After this, definition examples can be given to clarify the themes.</p>
                    </list-item>
                </list>
            </p>
            <p>Is the work clearly and accurately presented and does it cite the current literature?</p>
            <p>Partly</p>
            <p>If applicable, is the statistical analysis and its interpretation appropriate?</p>
            <p>Yes</p>
            <p>Are all the source data underlying the results available to ensure full reproducibility?</p>
            <p>Partly</p>
            <p>Is the study design appropriate and is the work technically sound?</p>
            <p>Partly</p>
            <p>Are the conclusions drawn adequately supported by the results?</p>
            <p>Yes</p>
            <p>Are sufficient details of methods and analysis provided to allow replication by others?</p>
            <p>No</p>
            <p>Reviewer Expertise:</p>
            <p>Science education</p>
            <p>I confirm that I have read this submission and believe that I have an appropriate level of expertise to confirm that it is of an acceptable scientific standard, however I have significant reservations, as outlined above.</p>
        </body>
    </sub-article>
    <sub-article article-type="reviewer-report" id="report152760">
        <front-stub>
            <article-id pub-id-type="doi">10.5256/f1000research.77288.r152760</article-id>
            <title-group>
                <article-title>Reviewer response for version 1</article-title>
            </title-group>
            <contrib-group>
                <contrib contrib-type="author">
                    <name>
                        <surname>Delaney</surname>
                        <given-names>Seamus</given-names>
                    </name>
                    <xref ref-type="aff" rid="r152760a1">1</xref>
                    <role>Referee</role>
                    <uri content-type="orcid">https://orcid.org/0000-0003-3271-1686</uri>
                </contrib>
                <aff id="r152760a1">
                    <label>1</label>Deakin University, Burwood, Australia</aff>
            </contrib-group>
            <author-notes>
                <fn fn-type="conflict">
                    <p>
                        <bold>Competing interests: </bold>No competing interests were disclosed.</p>
                </fn>
            </author-notes>
            <pub-date pub-type="epub">
                <day>20</day>
                <month>10</month>
                <year>2022</year>
            </pub-date>
            <permissions>
                <copyright-statement>Copyright: &#x00a9; 2022 Delaney S</copyright-statement>
                <copyright-year>2022</copyright-year>
                <license xlink:href="https://creativecommons.org/licenses/by/4.0/">
                    <license-p>This is an open access peer review report distributed under the terms of the Creative Commons Attribution Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.</license-p>
                </license>
            </permissions>
            <related-article ext-link-type="doi" id="relatedArticleReport152760" related-article-type="peer-reviewed-article" xlink:href="10.12688/f1000research.73624.1"/>
            <custom-meta-group>
                <custom-meta>
                    <meta-name>recommendation</meta-name>
                    <meta-value>reject</meta-value>
                </custom-meta>
            </custom-meta-group>
        </front-stub>
        <body>
            <p>Thank you to the author for making this contribution. The overall methodology presented appears somewhat haphazard (no control/intervention groups, sequential with the same text questions), and the student sample size appears too low to draw any statistically significant conclusions. However, I make some hopefully useful suggestions below that might make the subsequent submission a bit more robust, including focussing more on the qualitative data collected here (interviews) which appear to have good depth to them and so more useful then the test responses.</p>
            <p> </p>
            <p> It is perhaps not the remit of an international peer reviewer to make comment on another country&#x2019;s human ethics process, but it seems strange to that (a) only 
                <italic>verbal </italic>consent was collected before the participants and (b) this consent was collected 
                <italic>before</italic> approval to conduct the research was granted by the author&#x2019;s university. Could this be clarified by the author, as it might raise alarm bells for the readership. &#x00a0;</p>
            <p> </p>
            <p> In the methodology (page 4), the description of the cycles is confusing. It might be clearer to describe who here are the control group and who is the intervention group. However, there wasn&#x2019;t a control group was there? As it reads here, the same eight students completed the &#x2018;static&#x2019; test, and then the same ones completed the &#x2018;dynamic&#x2019; test. Could it not just be that the students who demonstrated &#x2018;correct understanding&#x2019; in test 2, needed more time to develop their conceptual understanding? What if they were drawing upon an experience in cycle 1 (the &#x2018;static&#x2019; Powerpoint) to answer a question in test 2? And if the test hasn&#x2019;t changed (same test questions both times), couldn&#x2019;t students have just improved because this was their second attempt?</p>
            <p> </p>
            <p> Figure 1 presents CO
                <sub>2</sub> molecules in a V/bent shape, rather than linear, with the oxygen atoms here significantly smaller than the oxygen atoms in the water molecule drawn in the same box. I&#x2019;m a little concerned about labelling this response as &#x2018;correct&#x2019;. But if the approach here was to evaluate on their conceptual understanding of stoichiometry, then okay.</p>
            <p> </p>
            <p> Could the author provide significantly more insight into what the students actually did in the &#x2018;dynamic&#x2019; approach? It is scantly described here in the main body of the text (the appendices are difficult the scan through) and so it is not immediately obvious what happened differently the second time.</p>
            <p> </p>
            <p> At the end of the discussion, the author describes how the &#x201c;visual mode of representation&#x201d; is under explored. This does seem to give short thrift to a significant amount of chemistry education research out there. However, I&#x2019;d like the author to consider more the choice of words here. Is this visual? The brief discussion presented here would seem to argue that the students benefitted from being able to &#x2018;touch and play&#x2019;, i.e. pick up models of molecules and manipulate them as they explore concepts of stoichiometry. This sounds more like a haptic experience than a visual one. Both the static and dynamic Powerpoint presentations are clearly visual one, but this is more to do here with the use of animation (or not). Could the author please explore their choice of language here. &#x00a0;</p>
            <p> </p>
            <p> I appreciate the sample size was very small, but there hasn&#x2019;t been any effort here to present these findings in any quantitative way. The reader is forced to make assumptions from how table 2a and table 2b is presented (more students got the questions correct the second time around) to gauge 
                <italic>how much</italic> the improvement was, and for which students. With such a small sample size perhaps it would have been more prudent to focus more on the interviews. For instance, analyse the interviews more deeply to unpack exactly what strategy they employed to answer correctly (or incorrectly) an individual question, and if this could be tied back to the learning intentions of the static (cycle 1) or dynamic (cycle 2) lessons. In short, this would have been better as a qualitative study than a mixed methods/quantitative study.</p>
            <p>Is the work clearly and accurately presented and does it cite the current literature?</p>
            <p>Partly</p>
            <p>If applicable, is the statistical analysis and its interpretation appropriate?</p>
            <p>No</p>
            <p>Are all the source data underlying the results available to ensure full reproducibility?</p>
            <p>Yes</p>
            <p>Is the study design appropriate and is the work technically sound?</p>
            <p>No</p>
            <p>Are the conclusions drawn adequately supported by the results?</p>
            <p>Partly</p>
            <p>Are sufficient details of methods and analysis provided to allow replication by others?</p>
            <p>Yes</p>
            <p>Reviewer Expertise:</p>
            <p>Chemistry education research</p>
            <p>I confirm that I have read this submission and believe that I have an appropriate level of expertise to state that I do not consider it to be of an acceptable scientific standard, for reasons outlined above.</p>
        </body>
    </sub-article>
</article>
