Determining the presence of trauma in forensic medicine is very important in assisting the criminal investigation process. ¹ Trauma cannot always be determined through external examination, so an internal examination (autopsy) is needed. ² Macroscopic examination of organs or body tissues that have been traumatized sometimes does not show satisfactory results. It is necessary to carry out a microscopic examination so that evidence of trauma is more accurate. ³ Forensic pathologists are always challenged to prove whether a person died as a result of trauma or not, and the opinion of the forensic pathologists is very important for medico legal purposes. ^{1 , 4} In determining the cause of death, including traumatic brain injury (TBI) is usually carried out by internal examination (autopsy), but this is still incomplete because it only leads to the level of epidemiological causes of death not yet leading to causality. ³ Histopathology changes in injuries such as TBI are still being discussed. In general, if TBI occurs when a victim has not died (premortem), then the histopathology changes will be obvious, but if there is postmortem, then there is no inflammatory response. ⁴

Although research on the human body has often been carried out in forensic medicine, the accuracy of whether or not trauma is present still needs to be improved. Given the similarities in anatomy and physiology with humans, experimental animals can be considered in translation research from biomedical to clinical. The frequency of using experimental animals in forensic medical research published in scientific journals is around 4%. ⁵ In order to investigate a premortem histopathology changes in TBI, rats were euthanized. The definition of a premortem is a condition that occurs shortly after death, but the time limit is still controversial. ⁶

TBI induces a complex cascade of immunologic or tissue inflammatory responses that mimic ischemic reperfusion injury. ⁷ Primary head injury and secondary head injury activate the release of cellular mediators in the form of prostaglandins, pro inflammatory cytokines, and free radicals. In secondary injuries, there are disturbances in the processes of metabolism and homeostasis of brain cell ions, intracranial hemodynamics, and cerebro-spinal fluid (CSF) compartments which begin after the trauma but are not clinically apparent immediately after the trauma. ^{7 , 8}

Deaths due to TBI are some of the most common cases encountered by forensic pathologists. TBI is routinely implicated in cases classified as accidents, as well as in suicides and homicides. Recently, cases of traumatic brain injury have increased in both developed and developing countries. Therefore, it is very useful for evaluating the clinical and pathological features of head injuries. To carry out an investigation of the causes of death in traumatic brain injury cases, a comprehensive examination is needed, one of which is histopathology findings. Histopathology changes in deaths from traumatic brain injury are very important to determine the qualification of whether there was a TBI before death. Therefore, it is necessary to carry out research related to the comparison of premortem histopathology findings using experimental rats with and without traumatic brain injury.

The aim of this study was to compare the results of premortem histopathological findings in animal model with or without traumatic brain injury. The target of the research is for forensic pathologists to be better able to provide evidence of traumatic brain injury to investigators so that the investigation process is optimally successful. The significance of this research is that it provides additional information for forensic pathologists in determining the mechanism of death if this research can later be applied to humans.

This study involved 12 rats for each treatment group with and without traumatic brain injury. Table 1 shows the development of rat body weight before acclimatization, after acclimatization and rat brain weight after treatment. Macroscopically, there were differences between the two treatment groups as can be seen in Figure 3. The histopathology findings in both groups of rats can be seen in Figure 4. Quantitatively, the processes of degeneration, necrosis, apoptosis, congestion, inflammatory cells and hemorrhage in the two groups can be seen in Table 2. The average quantitative processes of degeneration, necrosis, apoptosis, congestion, inflammatory cells and hemorrhage in all groups can be seen in Table 3.

Microscopically, there were differences between the two treatment groups as can be seen in Figure 3.

Histopathology findings in both groups of rats can be seen in Figure 4.

The histopathology changes in the brain organ in the non TBI group showed normal neuron cells ( Figure 4A). Changes in the TBI group showed a lot of damage to neuron cells that were necrotic and some experienced apoptosis, in addition to blood congestion (congestion), hemorrhage and visible degeneration of the brain ( Figure 4B). Quantitatively the process of degeneration, necrosis, apoptosis, congestion, inflammatory cells and hemorrhage in the five groups can be seen in Table 2.

The results of the statistical tests on histopathology changes in the form of degeneration, necrosis, congestion apoptosis, inflammatory cells and hemorrhage in all groups can be seen in Table 3.

The description of histopathology changes in the brain organs in the group of rat without TBI found that neuron cells looked normal although there were also degeneration (21.16 ± 2.56/FV), necrosis (5.75 ± 0.98/FV), apoptosis (2.91 ± 0.80/FV), congestion (0.91 ± 0.49/FV), inflammatory cells (4.58 ± 1.15/FV) and hemorrhage (2.41 ± 1.11/FV). Changes in the rat traumatic brain injury group showed a lot of damage to neuron cells in the form of degeneration (48.41 ± 3.27/FV), necrosis (36.66 ± 2.89/FV), apoptosis (18.91 ± 1.24/FV), congestion (2.50 ± 0.31/FV), inflammatory cells (11.41 ± 1.71/FV) and hemorrhage (10.08 ± 2.17/FV). Based on the results of statistical analysis, it can be seen that in all parameters there is a significant difference (p ≤ 0.001).

Traumatic brain injury is a condition of injury to the head caused by external mechanical forces which results in abnormalities in brain tissue. According to the path-mechanism of head injury, it is divided into primary injuries which are head injuries as a direct result of a trauma, which can be in the form of a direct impact or an acceleration-deceleration process of head movement or it can also be caused by a coup and counter-coup event. Secondary injuries are injuries that occur as a result of various pathological processes that arise as an advanced stage of primary brain damage in the form of bleeding, brain edema, ongoing neuronal damage, ischemia. and neuron-chemical changes. Abnormalities in brain tissue in humans can result in changes in physiological function of the brain and can lead to pathological and histopathology conditions of the brain. ^{9 – 11}

Clinically, these changes in brain function can cause clinical symptoms in the form of periods of loss or decreased level of consciousness, signs of memory loss immediately before or after the incident, neurological deficits in the form of muscle weakness, balance disturbances, changes in vision, paralysis, sensory loss, aphasia, and so on, and there is a change in mental status at the time of injury such as confusion, disorientation, slow thinking, and so on. This disorder can be permanent or temporary. ^{9 – 11}

In this study of the treatment of head injuries using the Feeney model, rats were dropped from a height of one meter with the right side as the fulcrum so that the right side of the brain experienced TBI. The desired sample is 12 rats. In this study, macroscopic rat brains showed the location of the lesion on the right brain. In TBI rats, bruises and abrasions appear on the surface of the brain, swelling (cerebral edema) on the surface of the brain, extensive subdural and subarachnoid hemorrhages, visible dilation of blood vessels, and visible bleeding from the cerebrum, cerebellum to the brainstem. ¹²

In rats that experienced TBI, bruises and abrasions appeared. Bruises indicate the location of a focal injury where the place is in contact with the surface of the blunt object that injured it. Blisters occur due to the displacement of the brain parenchyma and can also be combined with bruises on the surface of the brain. ¹³ In the TBI group rats appeared to have brain edema after 8 days of head injury treatment. Brain edema is an important risk factor for determining the prognosis of TBI, brain edema indicates an inflammatory reaction. ¹² Bleeding and hematoma formation occur as a result of torn brain blood vessels during head injury. Bleeding into the subarachnoid space is common in cases of cerebral vascular trauma after head injury. Intraventricular and intracerebral bleeding are also common after trauma. ^{12 , 13}

Microscopically, traumatic brain injury shows a lesion in the form of significant degeneration of neurons in the neocortical area with increased vacuolization. ¹⁴ The degenerating neuronal cells are characterized by bright eosinophilic cytoplasm with condensed nuclei, in addition, there is vacuolization inside the cells showing swelling/degeneration. Neuronal cells that experience death are characterized by shrinking cell nuclei and nuclei experiencing pyknotic or karyorrhexis. ¹⁵ Traumatic brain injury lesions in other cases also show extensive edema, neuronal necrosis, karyolysis and vacuolar changes, and widened inter-cell gaps. ¹⁶ In severe TBI, brain intraparenchymal hemorrhage may be found. Inflammation that occurs as a result of injury is a response from damaged tissue, triggering the release and activation of cytokines and chemokines as well as activation and proliferation of microglia. Elevated cytokines and other inflammatory mediators such as IL-1α, IL-6, and TNF-α were also confirmed in other studies of traumatic brain injury, in which the traumatic stress response was one of the causes. Metabolic disorders, including hypoxia and oxidative stress can lead to neuronal apoptosis, disruption of the blood brain barrier and brain edema following traumatic brain injury. ^{17 , 18}

Cell degeneration is a type of cell damage that occurs in the mitochondria and cytoplasm due to a minor injury that can be repaired if the cause is removed. This cell degeneration is a decline in cell function that interferes with cell metabolism which is reversible. ¹⁸ In this study all treatment groups showed different numbers of degenerated cells, statistically the ANOVA test found significant differences (p < 0.001).

Cell necrosis is a permanent cell in the form of dead tissue. If the degeneration is reversible, then necrosis is irreversible, which means the dead cells cannot be repaired. With histopathology examination, the presence of dead cells can be determined so that if a healing process is needed immediately can be handled by removing the dead cells. In TBI there is an obstacle to the supply of blood and oxygen to the cells so that the cells die. This condition is caused by various things, such as injury, infection, extreme environment, to certain medical conditions that cause damage to the cells so that the cells die and can no longer function normally. ^{18 , 19} The statistical test results using the Kruskal Wallis test obtained a significant value (p < 0.001) as in Table 3. This p value indicates that there were differences from all TBI treatment groups in causing necrosis, statistically significant differences were found because the p value < 0.05.

Apoptosis is a component of the series of events following cerebral trauma and one of the main pathways leading to cell death. If necrosis occurs for some reason, apoptosis generally lasts a lifetime. ²⁰ In this study all TBI treatment groups showed different numbers of cells undergoing apoptosis, statistically the ANOVA test found a significant difference (p = 0.001).

Congestion in general means there is blood damming in the tissue with a marked abundance of blood in the blood vessels in a particular region. Congestion is often also called hyperemia, when viewed microscopically the capillaries in the hyperemic tissue appear dilated and filled with blood. Cell congestion means there is congestion at the cellular level. ¹⁹ Investigating micro vascular congestion, blood vessels are carefully examined to see if they are small, medium or large containing red blood cells. ²¹ In this study on TBI rat, there were several variations in the level of cell congestion with significant differences for all groups. Statistical test results using the Kruskal Wallis test obtained a significant value (p = 0.001) as in Table 3. This p value indicates that there were differences from all TBI treatment groups in causing congestion, statistically significant differences were found because the p value < 0.05.

Inflammation is a natural response of the immune system when an injury or disease occurs. When rats experience TBI, they secrete inflammatory cells as a defense mechanism of the body that plays a role in the healing process of wounds which on histopathology examination are often called inflammatory cells. When mice get an infection due to TBI, there are many cells of the immune system that are involved, which then release various substances known as inflammatory mediators. ²² The statistical test results using the Kruskal Wallis test obtained a significant value (p = 0.001) as in Table 3. This p value indicates that there were differences from all TBI treatment groups in causing inflammatory cells to occur, statistically significant differences were found because the p value < 0.05.

Hemorrhage is the body’s response when injured by bleeding as a result of leaking blood vessels. ²³ In this study all treatment groups showed different numbers of hemorrhagic cells, statistically the ANOVA test found significant differences (p < 0.001). In this study, quantitative differences were seen in degeneration, necrosis, apoptosis, congestion, inflammatory cells and hemorrhage in rat brain cells in each treatment. This shows a different inflammatory response from each treatment. The greater the number of degeneration, necrosis, congestion apoptosis, inflammatory cells and hemorrhage in rat brain cells, the greater the severity of inflammation caused by traumatic brain injury in rats.

Determining whether or not TBI is very important in the practice of forensic medicine. The results of histopathology analysis can be an alternative to determining causality in a short period of time at low cost. However, in the not TBI group, there were also histopathology changes indicating a different mechanism in the not TBI group. This study showed an increase in the level of cell damage in the TBI group compared to the not TBI group. Sequentially the comparison of the TBI group with the not TBI group is degeneration (2.28;1), necrosis (6.38:1), apoptosis (6.28:1), congestion (2.72:1), inflammatory cells (2.49:1) and hemorrhage (4.17:1).

The limitation of the study is that it has limited literature on premortem histopathology so that the discussion in this study is less than optimal. So further research is needed to increase the amount of literature.

This research is in line with studies conducted by Ibrahim et al., which stated that there were signs of inflammation in the form of haemorrhage and inflammation as well as necrosis in tissue as the body's response to significant antemortem stress, this also showed enzymatic changes in tissue. Meanwhile, for postmortem cells, there is tissue necrosis and severe cell damage. A combination of antemortem and postmortem histopathology showed changes in fatality in mice subjected to hypothermia. ²⁴

This research is also in line with the study conducted by Al Salh & Al-Jameel, which stated the difference between antemortem and postmortem wounds, with antemortem characteristics being bleeding, increased macrophages and neutrophils, while postmortem showed tissue degeneration, bleeding and autolysis. ⁴

We conclude that the damage to neuronal cells is more severe in premortem rats with traumatic brain injury, especially necrosis, apoptosis and hemorrhage. There is a significant difference between premortem histopathology findings in rats with and without traumatic brain injury which can be used for the benefit of forensic medicine in determining whether TBI is present or not. It can be said that to determine the presence of TBI, it is necessary to look more closely at the histopathology changes in the form of necrosis, apoptosis and hemorrhage. The results of this study are relevant in animal models, further research will be carried out to find their relevance in human subjects so that they are useful for forensic medicine. In relation to determining the mechanism of death due to traumatic brain injury, a histopathological examination is needed to ensure a stronger causal relationship.