<?xml version="1.0" encoding="UTF-8"?><!DOCTYPE article PUBLIC "-//NLM//DTD JATS (Z39.96) Journal Publishing DTD v1.2 20190208//EN" "http://jats.nlm.nih.gov/publishing/1.2/JATS-journalpublishing1.dtd"><article xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" article-type="research-article" dtd-version="1.2" xml:lang="en">
    <front>
        <journal-meta>
            <journal-id journal-id-type="pmc">F1000Research</journal-id>
            <journal-title-group>
                <journal-title>F1000Research</journal-title>
            </journal-title-group>
            <issn pub-type="epub">2046-1402</issn>
            <publisher>
                <publisher-name>F1000 Research Limited</publisher-name>
                <publisher-loc>London, UK</publisher-loc>
            </publisher>
        </journal-meta>
        <article-meta>
            <article-id pub-id-type="doi">10.12688/f1000research.139603.1</article-id>
            <article-categories>
                <subj-group subj-group-type="heading">
                    <subject>Research Article</subject>
                </subj-group>
                <subj-group>
                    <subject>Articles</subject>
                </subj-group>
            </article-categories>
            <title-group>
                <article-title>Depression, anxiety, and stress among urban and rural adolescents in Shivamogga, Karnataka</article-title>
                <fn-group content-type="pub-status">
                    <fn>
                        <p>[version 1; peer review: 1 approved with reservations]</p>
                    </fn>
                </fn-group>
            </title-group>
            <contrib-group>
                <contrib contrib-type="author" corresp="yes">
                    <name>
                        <surname>Mallya</surname>
                        <given-names>Ajay</given-names>
                    </name>
                    <role content-type="http://credit.niso.org/">Conceptualization</role>
                    <role content-type="http://credit.niso.org/">Data Curation</role>
                    <role content-type="http://credit.niso.org/">Formal Analysis</role>
                    <role content-type="http://credit.niso.org/">Investigation</role>
                    <role content-type="http://credit.niso.org/">Methodology</role>
                    <role content-type="http://credit.niso.org/">Software</role>
                    <role content-type="http://credit.niso.org/">Supervision</role>
                    <role content-type="http://credit.niso.org/">Validation</role>
                    <role content-type="http://credit.niso.org/">Visualization</role>
                    <role content-type="http://credit.niso.org/">Writing &#x2013; Original Draft Preparation</role>
                    <role content-type="http://credit.niso.org/">Writing &#x2013; Review &amp; Editing</role>
                    <uri content-type="orcid">https://orcid.org/0000-0002-2280-3176</uri>
                    <xref ref-type="corresp" rid="c1">a</xref>
                    <xref ref-type="aff" rid="a1">1</xref>
                </contrib>
                <contrib contrib-type="author" corresp="no">
                    <name>
                        <surname>Koppad</surname>
                        <given-names>Raghavendraswamy</given-names>
                    </name>
                    <role content-type="http://credit.niso.org/">Conceptualization</role>
                    <role content-type="http://credit.niso.org/">Methodology</role>
                    <role content-type="http://credit.niso.org/">Software</role>
                    <role content-type="http://credit.niso.org/">Visualization</role>
                    <role content-type="http://credit.niso.org/">Writing &#x2013; Review &amp; Editing</role>
                    <xref ref-type="aff" rid="a2">2</xref>
                </contrib>
                <contrib contrib-type="author" corresp="no">
                    <name>
                        <surname>Kumar</surname>
                        <given-names>Praveen</given-names>
                    </name>
                    <role content-type="http://credit.niso.org/">Conceptualization</role>
                    <role content-type="http://credit.niso.org/">Validation</role>
                    <role content-type="http://credit.niso.org/">Writing &#x2013; Original Draft Preparation</role>
                    <xref ref-type="aff" rid="a2">2</xref>
                </contrib>
                <aff id="a1">
                    <label>1</label>Department of Community Medicine, Kasturba Medical College, Mangalore, Manipal Academy of Higher Education, Manipal, India</aff>
                <aff id="a2">
                    <label>2</label>Department of Community Medicine, Shivamogga Institute of Medical Sciences, Shivamogga, Karnataka, 577201, India</aff>
            </contrib-group>
            <author-notes>
                <corresp id="c1">
                    <label>a</label>
                    <email xlink:href="mailto:ajay.mallya@manipal.edu">ajay.mallya@manipal.edu</email>
                </corresp>
                <fn fn-type="conflict">
                    <p>No competing interests were disclosed.</p>
                </fn>
            </author-notes>
            <pub-date pub-type="epub">
                <day>14</day>
                <month>12</month>
                <year>2023</year>
            </pub-date>
            <pub-date pub-type="collection">
                <year>2023</year>
            </pub-date>
            <volume>12</volume>
            <elocation-id>1583</elocation-id>
            <history>
                <date date-type="accepted">
                    <day>26</day>
                    <month>9</month>
                    <year>2023</year>
                </date>
            </history>
            <permissions>
                <copyright-statement>Copyright: &#x00a9; 2023 Mallya A et al.</copyright-statement>
                <copyright-year>2023</copyright-year>
                <license xlink:href="https://creativecommons.org/licenses/by/4.0/">
                    <license-p>This is an open access article distributed under the terms of the Creative Commons Attribution Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.</license-p>
                </license>
            </permissions>
            <self-uri content-type="pdf" xlink:href="https://f1000research.com/articles/12-1583/pdf"/>
            <abstract>
                <p>
                    <bold>Background:</bold> Currently there are 1.3 billion adolescents worldwide, which makes up 16% of the world population. Over 20% of adolescents around the world are thought to have behavioural or mental health issues. Addressing mental health issues is very important for the promotion of positive health in adolescents.</p>
                <p>
                    <bold>Methods:</bold> The objective of our study was to estimate the prevalence of depression, anxiety, and stress among adolescents in urban and rural areas of Shivamogga. A cross-sectional study was conducted among 350 adolescents aged 16 to 19 years each from urban and rural areas of Shivamogga.</p>
                <p>
                    <bold>Results:</bold> Depression, anxiety and stress were found to be 23.1%, 29.4% and 26.6% in urban areas and 19.1%, 24% and 21.1% in rural areas respectively.</p>
                <p>
                    <bold>Conclusions:</bold> About a quarter of the adolescent population suffers from depression anxiety and stress. Adopting and implementing better education and health policies are necessary to enhance adolescent mental health.</p>
            </abstract>
            <kwd-group kwd-group-type="author">
                <kwd>Mental health</kwd>
                <kwd>Adolescence</kwd>
                <kwd>Depression</kwd>
                <kwd>anxiety</kwd>
                <kwd>stress</kwd>
            </kwd-group>
            <funding-group>
                <funding-statement>The author(s) declared that no grants were involved in supporting this work.</funding-statement>
            </funding-group>
        </article-meta>
    </front>
    <body>
        <def-list>
            <title>Abbreviations</title>
            <def-item>
                <term id="G1">DALYs</term>
                <def>
                    <p>Disability Adjusted Life Years</p>
                </def>
            </def-item>
            <def-item>
                <term id="G2">DASS</term>
                <def>
                    <p>Depression Anxiety Stress scale</p>
                </def>
            </def-item>
            <def-item>
                <term id="G3">SEAR</term>
                <def>
                    <p>South East Asia Region</p>
                </def>
            </def-item>
            <def-item>
                <term id="G4">SPSS</term>
                <def>
                    <p>Statistical Package for Social Sciences</p>
                </def>
            </def-item>
            <def-item>
                <term id="G5">WHO</term>
                <def>
                    <p>World Health Organization</p>
                </def>
            </def-item>
        </def-list>
        <sec id="sec1" sec-type="intro">
            <title>Introduction</title>
            <p>Currently, there are 1.3 billion adolescents worldwide, which makes up 16% of the world population.
                <sup>
                    <xref ref-type="bibr" rid="ref1">1</xref>
                </sup> Adolescents make up 20% of the population in countries in the South-East Asia region (SEAR) of the World Health Organization (WHO), and 21.8% of the Indian population.
                <sup>
                    <xref ref-type="bibr" rid="ref2">2</xref>
                </sup>
                <sup>,</sup>
                <sup>
                    <xref ref-type="bibr" rid="ref3">3</xref>
                </sup>
            </p>
            <p>Adolescence is a distinct period in human development and crucial for setting the groundwork for long-term health. Today nine out of ten adolescents are living in underdeveloped countries, where they confront particularly difficult obstacles in everyday lives from getting an education to simply surviving.
                <sup>
                    <xref ref-type="bibr" rid="ref4">4</xref>
                </sup> Over 20% of adolescents around the world are thought to have behavioural or mental health issues. Lifetime mental health disorders commonly begin by the age of 14, and depression is the leading cause of the global disease burden among adolescents.
                <sup>
                    <xref ref-type="bibr" rid="ref4">4</xref>
                </sup>
            </p>
            <p>Adolescents frequently suffer from emotional disturbances. Anxiety disorders predominate among adolescents and are more frequently seen in older adolescents compared to younger ones. Anxiety disorders are thought to affect 3.6% of 10&#x2013;14-year-olds and 4.6% of 15&#x2013;19-year-olds.
                <sup>
                    <xref ref-type="bibr" rid="ref5">5</xref>
                </sup> Depression and anxiety share some common features and together can result in poor performance in school and poor school attendance.
                <sup>
                    <xref ref-type="bibr" rid="ref5">5</xref>
                </sup> This, in addition to the marked social impairment experienced by depressed adolescents, will result in poor workplace relationships which in turn leads to poor connections and can affect one&#x2019;s ability to make academic and workplace progress. Depression in adolescents also decreases the chance of them pursuing higher education and can also lead to lower employment rates. Depression in adolescents is also associated with earlier pregnancy and early parenthood.
                <sup>
                    <xref ref-type="bibr" rid="ref6">6</xref>
                </sup>
            </p>
            <p>Adolescents with depression run a very high risk of suicide.
                <sup>
                    <xref ref-type="bibr" rid="ref7">7</xref>
                </sup> Smoking, substance abuse, and obesity are all also more prevalent among adolescents who are depressed.
                <sup>
                    <xref ref-type="bibr" rid="ref8">8</xref>
                </sup>
                <sup>,</sup>
                <sup>
                    <xref ref-type="bibr" rid="ref9">9</xref>
                </sup> Depressive disorders are among the top three causes of adolescent disability adjusted life years (DALYs) lost globally and anxiety happens to be among the top five causes of loss of DALYs among adolescent girls.
                <sup>
                    <xref ref-type="bibr" rid="ref10">10</xref>
                </sup> About 7% of adolescents between the ages of 13 and 17 suffer from diagnosed mental health disorders in India according to the National Mental Health Survey 2015-16.
                <sup>
                    <xref ref-type="bibr" rid="ref11">11</xref>
                </sup>
            </p>
            <p>Stress in adolescents also affects their academic performance.
                <sup>
                    <xref ref-type="bibr" rid="ref12">12</xref>
                </sup> Levels of stress experienced are shown to increase from preadolescence to adolescence.
                <sup>
                    <xref ref-type="bibr" rid="ref13">13</xref>
                </sup> Studies have shown that girls tend to experience higher levels of stress compared to boys during adolescence.
                <sup>
                    <xref ref-type="bibr" rid="ref14">14</xref>
                </sup> There is evidence that shows a positive correlation between stress and depression and anxiety.
                <sup>
                    <xref ref-type="bibr" rid="ref15">15</xref>
                </sup>
            </p>
            <p>Addressing mental health issues is very important for the promotion of positive health in adolescents. It also facilitates a whole generation of adults with sound mental health and leads to a healthy society. And as we couldn&#x2019;t identify any studies done on mental health issues in this region of Karnataka, there is a need to carry out research and gather more data and facilitate the promotion of positive mental health among adolescents in this part of Karnataka.</p>
        </sec>
        <sec id="sec2" sec-type="methods">
            <title>Methods</title>
            <sec id="sec3">
                <title>Ethics and consent</title>
                <p>Ethical approval was granted by the Institutional Ethics Committee of Shivamogga Institute of medical Sciences, Shivamogga (ref no. SIMS/IEC/414) on 09/11/2018. Informed consent was taken from study participants and the parents/guardians in case of minors (16-17 years) via Google Forms. Assent was also taken from study participants of age 16-17 years.</p>
            </sec>
            <sec id="sec4">
                <title>Study design and data collection</title>
                <p>A cross-sectional study was conducted in urban and rural areas of Shivamogga taluk to estimate the prevalence of depression, anxiety, and stress among adolescents. This study was conducted from August 2020 to September 2020. The study population is composed of all adolescent males and females aged 16-19 years in rural and urban field areas of Shivamogga taluk. According to an Indian study, the prevalence (p) of anxiety among adolescents was found to be 24.4%.
                    <sup>
                        <xref ref-type="bibr" rid="ref16">16</xref>
                    </sup> With desired absolute precision of 5% the minimum sample size required was calculated using the formula n=4pq/D2. With this, we got a sample size of 295 adolescents. Considering 10% as non-respondents, the final sample size was 325 which was rounded off to 350. So, 350 samples were collected in urban and rural areas each.</p>
                <p>The Block education officer was approached and a list of all pre-university colleges in Shivamogga taluk was collected. Shivamogga taluk has 36 pre-university colleges in the urban area and 10 pre-university colleges in the rural area. Then 50% of the pre-university colleges were randomly selected using the random number table both in urban and rural areas. So, 18 colleges from the urban and 5 colleges from the rural area were selected. From each selected colleges, a class was randomly selected. For each selected class the lottery method was used to select 20 students from each class in the urban area and 70 students from the rural area until the desired sample size was reached. All adolescents in the age group of 16 to 19 years were included in the study. Adolescents who did not fill out the questionnaire completely were excluded from the study.</p>
                <p>After taking consent, a questionnaire that included basic demographic details (age and sex) with the DASS 21 scale (Depression Anxiety Stress Scale)
                    <sup>
                        <xref ref-type="bibr" rid="ref17">17</xref>
                    </sup> was used to collect data. A Google Form of the questionnaire was created, one each for urban and rural areas and the link to fill out the form was shared with students willing to participate in the study via &#x2018;WhatsApp&#x2019; mobile application. Adolescents aged 16 completed years and above until the age of 19 were included in the study.</p>
            </sec>
            <sec id="sec5">
                <title>Statistical analysis</title>
                <p>Data was entered into a Microsoft Excel spreadsheet. SPSS (Version 21) was used to perform statistical analysis. Data analysis was done using appropriate statistical tools. Results were expressed in terms of frequency, percentages, Chi-square values, and P-values. All statistical tests were performed with a confidence level of 95% and a power of 80%. In the significance tests, p-values less than 0.05 were considered significant.</p>
            </sec>
        </sec>
        <sec id="sec6" sec-type="results">
            <title>Results</title>
            <p>As shown in 
                <xref ref-type="table" rid="T1">Table 1</xref>: a total of 700 adolescents participated in the study. Out of which 350 (50%) adolescents were from urban areas and another 350 (50%) adolescents were from rural areas. Males and females in urban areas were 153 (44%) and 197 (56%) respectively. In rural areas, males and females were 191 (55%) and 159 (45%) respectively.</p>
            <table-wrap id="T1" orientation="portrait" position="float">
                <label>Table 1. </label>
                <caption>
                    <title>Distribution of study participants according to depression, anxiety and stressed in urban and rural areas. (n=700).</title>
                </caption>
                <table content-type="article-table" frame="hsides">
                    <thead>
                        <tr>
                            <th align="left" colspan="1" rowspan="1" valign="top">Sl. No.</th>
                            <th align="left" colspan="1" rowspan="1" valign="top">Characteristics</th>
                            <th align="left" colspan="1" rowspan="1" valign="top">Urban</th>
                            <th align="left" colspan="1" rowspan="1" valign="top">Rural</th>
                            <th align="left" colspan="1" rowspan="1" valign="top">P value</th>
                        </tr>
                    </thead>
                    <tbody>
                        <tr>
                            <td align="left" colspan="1" rowspan="3" valign="middle">1</td>
                            <td align="left" colspan="1" rowspan="1" valign="middle">No. of participants</td>
                            <td colspan="1" rowspan="1"/>
                            <td colspan="1" rowspan="1"/>
                            <td align="left" colspan="1" rowspan="3" valign="middle">0.004</td>
                        </tr>
                        <tr>
                            <td align="left" colspan="1" rowspan="1" valign="middle">&#x2022; Males</td>
                            <td align="left" colspan="1" rowspan="1" valign="middle">153 (44%)</td>
                            <td align="left" colspan="1" rowspan="1" valign="middle">191 (55%)</td>
                        </tr>
                        <tr>
                            <td align="left" colspan="1" rowspan="1" valign="middle">&#x2022; Females</td>
                            <td align="left" colspan="1" rowspan="1" valign="middle">197 (56%)</td>
                            <td align="left" colspan="1" rowspan="1" valign="middle">159 (45%)</td>
                        </tr>
                        <tr>
                            <td align="left" colspan="1" rowspan="5" valign="middle">2</td>
                            <td align="left" colspan="1" rowspan="1" valign="middle">Age of the participants</td>
                            <td colspan="1" rowspan="1"/>
                            <td colspan="1" rowspan="1"/>
                            <td align="left" colspan="1" rowspan="5" valign="middle">0.011</td>
                        </tr>
                        <tr>
                            <td align="left" colspan="1" rowspan="1" valign="middle">&#x2022; 16 years</td>
                            <td align="left" colspan="1" rowspan="1" valign="middle">94 (27%)</td>
                            <td align="left" colspan="1" rowspan="1" valign="middle">60 (17%)</td>
                        </tr>
                        <tr>
                            <td align="left" colspan="1" rowspan="1" valign="middle">&#x2022; 17 years</td>
                            <td align="left" colspan="1" rowspan="1" valign="middle">142 (40.6%)</td>
                            <td align="left" colspan="1" rowspan="1" valign="middle">159 (45%)</td>
                        </tr>
                        <tr>
                            <td align="left" colspan="1" rowspan="1" valign="middle">&#x2022; 18 years</td>
                            <td align="left" colspan="1" rowspan="1" valign="middle">100 (29%)</td>
                            <td align="left" colspan="1" rowspan="1" valign="middle">121 (35%)</td>
                        </tr>
                        <tr>
                            <td align="left" colspan="1" rowspan="1" valign="middle">&#x2022; 19 years</td>
                            <td align="left" colspan="1" rowspan="1" valign="middle">14 (4%)</td>
                            <td align="left" colspan="1" rowspan="1" valign="middle">19 (3%)</td>
                        </tr>
                        <tr>
                            <td align="left" colspan="1" rowspan="5" valign="middle">3</td>
                            <td align="left" colspan="1" rowspan="1" valign="middle">Depression</td>
                            <td colspan="1" rowspan="1"/>
                            <td colspan="1" rowspan="1"/>
                            <td align="left" colspan="1" rowspan="5" valign="middle">0.195</td>
                        </tr>
                        <tr>
                            <td align="left" colspan="1" rowspan="1" valign="middle">&#x2022; Mild</td>
                            <td align="left" colspan="1" rowspan="1" valign="middle">25 (7.1%)</td>
                            <td align="left" colspan="1" rowspan="1" valign="middle">32 (9.1%)</td>
                        </tr>
                        <tr>
                            <td align="left" colspan="1" rowspan="1" valign="middle">&#x2022; Moderate</td>
                            <td align="left" colspan="1" rowspan="1" valign="middle">46 (13.1%)</td>
                            <td align="left" colspan="1" rowspan="1" valign="middle">28 (8%)</td>
                        </tr>
                        <tr>
                            <td align="left" colspan="1" rowspan="1" valign="middle">&#x2022; Severe</td>
                            <td align="left" colspan="1" rowspan="1" valign="middle">10 (2.9%)</td>
                            <td align="left" colspan="1" rowspan="1" valign="middle">7 (2%)</td>
                        </tr>
                        <tr>
                            <td align="left" colspan="1" rowspan="1" valign="middle">&#x2022; Not depressed</td>
                            <td align="left" colspan="1" rowspan="1" valign="middle">269 (76.9%)</td>
                            <td align="left" colspan="1" rowspan="1" valign="middle">283 (80.9%)</td>
                        </tr>
                        <tr>
                            <td align="left" colspan="1" rowspan="5" valign="middle">4</td>
                            <td align="left" colspan="1" rowspan="1" valign="middle">Anxiety</td>
                            <td colspan="1" rowspan="1"/>
                            <td colspan="1" rowspan="1"/>
                            <td align="left" colspan="1" rowspan="5" valign="middle">0.105</td>
                        </tr>
                        <tr>
                            <td align="left" colspan="1" rowspan="1" valign="middle">&#x2022; Mild</td>
                            <td align="left" colspan="1" rowspan="1" valign="middle">29 (8.2%)</td>
                            <td align="left" colspan="1" rowspan="1" valign="middle">21 (6%)</td>
                        </tr>
                        <tr>
                            <td align="left" colspan="1" rowspan="1" valign="middle">&#x2022; Moderate</td>
                            <td align="left" colspan="1" rowspan="1" valign="middle">71 (20.3%)</td>
                            <td align="left" colspan="1" rowspan="1" valign="middle">58 (16.6%)</td>
                        </tr>
                        <tr>
                            <td align="left" colspan="1" rowspan="1" valign="middle">&#x2022; Severe</td>
                            <td align="left" colspan="1" rowspan="1" valign="middle">3 (0.9%)</td>
                            <td align="left" colspan="1" rowspan="1" valign="middle">5 (1.4%)</td>
                        </tr>
                        <tr>
                            <td align="left" colspan="1" rowspan="1" valign="middle">&#x2022; Not anxious</td>
                            <td align="left" colspan="1" rowspan="1" valign="middle">247 (70.6%)</td>
                            <td align="left" colspan="1" rowspan="1" valign="middle">266 (76%)</td>
                        </tr>
                        <tr>
                            <td align="left" colspan="1" rowspan="5" valign="middle">5</td>
                            <td align="left" colspan="1" rowspan="1" valign="middle">Stress</td>
                            <td colspan="1" rowspan="1"/>
                            <td colspan="1" rowspan="1"/>
                            <td align="left" colspan="1" rowspan="5" valign="middle">0.092</td>
                        </tr>
                        <tr>
                            <td align="left" colspan="1" rowspan="1" valign="middle">&#x2022; Mild</td>
                            <td align="left" colspan="1" rowspan="1" valign="middle">63 (18%)</td>
                            <td align="left" colspan="1" rowspan="1" valign="middle">54 (15.4%)</td>
                        </tr>
                        <tr>
                            <td align="left" colspan="1" rowspan="1" valign="middle">&#x2022; Moderate</td>
                            <td align="left" colspan="1" rowspan="1" valign="middle">24 (6.9%)</td>
                            <td align="left" colspan="1" rowspan="1" valign="middle">13 (3.7%)</td>
                        </tr>
                        <tr>
                            <td align="left" colspan="1" rowspan="1" valign="middle">&#x2022; Severe</td>
                            <td align="left" colspan="1" rowspan="1" valign="middle">6 (1.7%)</td>
                            <td align="left" colspan="1" rowspan="1" valign="middle">7 (2%)</td>
                        </tr>
                        <tr>
                            <td align="left" colspan="1" rowspan="1" valign="middle">&#x2022; Not stressed</td>
                            <td align="left" colspan="1" rowspan="1" valign="middle">257 (73.4%)</td>
                            <td align="left" colspan="1" rowspan="1" valign="middle">276 (78.9%)</td>
                        </tr>
                    </tbody>
                </table>
            </table-wrap>
            <p>All our study participants were aged between 16 to 19 years. the mean age of the participants in the urban area was 17.10&#x00b1;0.84 years. In the urban area, the majority of the participants (142) were aged 17 years (40.6%). Participants aged 16 years were 94 (27%), 18 years were 100 (29%) and 19 years were 14 (4%). Similarly in rural areas, the mean age of the participants was 17.10 &#x00b1;0.76 years. The majority (159) of the participants were aged 17 years (45%). Participants aged 16 years were 60 (17%), 18 years were 121 (35%) and 19 years were 10 (3%).</p>
            <p>Among the adolescents in urban areas, 23.1% were depressed, 29.4% had anxiety and 26.6% had stress. Similarly, among the adolescents in rural areas, 19.1% were depressed, 24% had anxiety and 21.1% were stressed.</p>
            <p>Among the depressed adolescents, in urban areas the majority (46; 13.1%) had moderate depression, whereas in rural areas the majority (32; 9.1%) had mild depression. Among the adolescents who had anxiety, both in urban and rural areas the majority (71 (20.3%) and 58 (16.6%) respectively) had moderate anxiety. Among the adolescents who were stressed, both in urban and rural areas, the majority (63 (18%) and 54 (15.4%) respectively) had mild stress.</p>
            <p>Among the study participants from urban areas of Shivamogga, 33 (21.6%) adolescent males and 48 (24.4%) adolescent females were depressed, 33 (21.6%) adolescent males and 70 (35.5%) adolescent females were anxious, and 55 (35.9%) adolescent males and 38 (19.3) adolescent females were stressed (see 
                <xref ref-type="table" rid="T2">Table 2</xref>). A significantly greater number of females were anxious and stressed than males.</p>
            <table-wrap id="T2" orientation="portrait" position="float">
                <label>Table 2. </label>
                <caption>
                    <title>Distribution of study participants based on sex in urban areas of Shivamogga (n=350).</title>
                </caption>
                <table content-type="article-table" frame="hsides">
                    <thead>
                        <tr>
                            <th align="left" colspan="1" rowspan="1" valign="top">Sl. No.</th>
                            <th align="left" colspan="1" rowspan="1" valign="top">Characteristics</th>
                            <th align="left" colspan="1" rowspan="1" valign="top">Males</th>
                            <th align="left" colspan="1" rowspan="1" valign="top">Females</th>
                            <th align="left" colspan="1" rowspan="1" valign="top">P value</th>
                        </tr>
                    </thead>
                    <tbody>
                        <tr>
                            <td align="left" colspan="1" rowspan="2" valign="middle">1</td>
                            <td align="left" colspan="1" rowspan="1" valign="middle">Not depressed</td>
                            <td align="left" colspan="1" rowspan="1" valign="middle">120 (78.4%)</td>
                            <td align="left" colspan="1" rowspan="1" valign="middle">149 (75.6%)</td>
                            <td align="left" colspan="1" rowspan="2" valign="middle">0.538</td>
                        </tr>
                        <tr>
                            <td align="left" colspan="1" rowspan="1" valign="middle">Depressed</td>
                            <td align="left" colspan="1" rowspan="1" valign="middle">33 (21.6%)</td>
                            <td align="left" colspan="1" rowspan="1" valign="middle">48 (24.4%)</td>
                        </tr>
                        <tr>
                            <td align="left" colspan="1" rowspan="2" valign="middle">2</td>
                            <td align="left" colspan="1" rowspan="1" valign="middle">Not anxious</td>
                            <td align="left" colspan="1" rowspan="1" valign="middle">120 (78.4%)</td>
                            <td align="left" colspan="1" rowspan="1" valign="middle">127 (64.5%)</td>
                            <td align="left" colspan="1" rowspan="2" valign="middle">0.005</td>
                        </tr>
                        <tr>
                            <td align="left" colspan="1" rowspan="1" valign="middle">Anxious</td>
                            <td align="left" colspan="1" rowspan="1" valign="middle">33 (21.6%)</td>
                            <td align="left" colspan="1" rowspan="1" valign="middle">70 (35.5%)</td>
                        </tr>
                        <tr>
                            <td align="left" colspan="1" rowspan="2" valign="middle">3</td>
                            <td align="left" colspan="1" rowspan="1" valign="middle">Not stressed</td>
                            <td align="left" colspan="1" rowspan="1" valign="middle">98 (64.1%)</td>
                            <td align="left" colspan="1" rowspan="1" valign="middle">159 (80.7%)</td>
                            <td align="left" colspan="1" rowspan="2" valign="middle">&lt;0.001</td>
                        </tr>
                        <tr>
                            <td align="left" colspan="1" rowspan="1" valign="middle">Stressed</td>
                            <td align="left" colspan="1" rowspan="1" valign="middle">55 (35.9%)</td>
                            <td align="left" colspan="1" rowspan="1" valign="middle">38 (19.3%)</td>
                        </tr>
                    </tbody>
                </table>
            </table-wrap>
            <p>Among the study participants from rural areas of Shivamogga, 26 (13.6%) adolescent males and 41 (25.8%) adolescent females were depressed, 40 (20.9%) adolescent males and 44 (27.7%) adolescent females were anxious, and 47 (24.6%) adolescent males and 27 (17%) adolescent females adolescents were stressed. A significantly greater number of females were stressed than males; see 
                <xref ref-type="table" rid="T3">Table 3</xref>.</p>
            <table-wrap id="T3" orientation="portrait" position="float">
                <label>Table 3. </label>
                <caption>
                    <title>Distribution of study participants based on sex in rural areas of Shivamogga (n=350).</title>
                </caption>
                <table content-type="article-table" frame="hsides">
                    <thead>
                        <tr>
                            <th align="left" colspan="1" rowspan="1" valign="top">Sl. No.</th>
                            <th align="left" colspan="1" rowspan="1" valign="top">Characteristics</th>
                            <th align="left" colspan="1" rowspan="1" valign="top">Males</th>
                            <th align="left" colspan="1" rowspan="1" valign="top">Females</th>
                            <th align="left" colspan="1" rowspan="1" valign="top">P Value</th>
                        </tr>
                    </thead>
                    <tbody>
                        <tr>
                            <td align="left" colspan="1" rowspan="2" valign="middle">1</td>
                            <td align="left" colspan="1" rowspan="1" valign="middle">Not depressed</td>
                            <td align="left" colspan="1" rowspan="1" valign="middle">165 (86.4%)</td>
                            <td align="left" colspan="1" rowspan="1" valign="middle">118 (74.2%)</td>
                            <td align="left" colspan="1" rowspan="2" valign="middle">0.004</td>
                        </tr>
                        <tr>
                            <td align="left" colspan="1" rowspan="1" valign="middle">Depressed</td>
                            <td align="left" colspan="1" rowspan="1" valign="middle">26 (13.6%)</td>
                            <td align="left" colspan="1" rowspan="1" valign="middle">41 (25.8%)</td>
                        </tr>
                        <tr>
                            <td align="left" colspan="1" rowspan="2" valign="middle">2</td>
                            <td align="left" colspan="1" rowspan="1" valign="middle">Not anxious</td>
                            <td align="left" colspan="1" rowspan="1" valign="middle">151 (79.1%)</td>
                            <td align="left" colspan="1" rowspan="1" valign="middle">115 (72.3%)</td>
                            <td align="left" colspan="1" rowspan="2" valign="middle">0.167</td>
                        </tr>
                        <tr>
                            <td align="left" colspan="1" rowspan="1" valign="middle">Anxious</td>
                            <td align="left" colspan="1" rowspan="1" valign="middle">40 (20.9%)</td>
                            <td align="left" colspan="1" rowspan="1" valign="middle">44 (27.7%)</td>
                        </tr>
                        <tr>
                            <td align="left" colspan="1" rowspan="2" valign="middle">3</td>
                            <td align="left" colspan="1" rowspan="1" valign="middle">Not stressed</td>
                            <td align="left" colspan="1" rowspan="1" valign="middle">144 (75.4%)</td>
                            <td align="left" colspan="1" rowspan="1" valign="middle">132 (83%)</td>
                            <td align="left" colspan="1" rowspan="2" valign="middle">0.089</td>
                        </tr>
                        <tr>
                            <td align="left" colspan="1" rowspan="1" valign="middle">Stressed</td>
                            <td align="left" colspan="1" rowspan="1" valign="middle">47 (24.6%)</td>
                            <td align="left" colspan="1" rowspan="1" valign="middle">27 (17%)</td>
                        </tr>
                    </tbody>
                </table>
            </table-wrap>
        </sec>
        <sec id="sec7" sec-type="discussion">
            <title>Discussion</title>
            <p>Our study attempted to find out the prevalence of depression, anxiety, and stress among adolescents in urban and rural areas. The current study found that the prevalence of depression was 23.13% and 19.14% in urban and rural areas respectively. On average, depression among adolescents in Shivamogga is 21.14%. Similar results were found by Kumar K S et al. and Sahoo S, where depression among adolescents was 19.5%. and 18.5% respectively.
                <sup>
                    <xref ref-type="bibr" rid="ref16">16</xref>
                </sup>
                <sup>,</sup>
                <sup>
                    <xref ref-type="bibr" rid="ref18">18</xref>
                </sup> Depression was seen to be higher in females in both urban and rural areas. The difference was statistically significant in rural areas of Shivamogga. Similar observations were made in other studies across India where depression was seen to be higher among female respondents.
                <sup>
                    <xref ref-type="bibr" rid="ref16">16</xref>
                </sup>
                <sup>,</sup>
                <sup>
                    <xref ref-type="bibr" rid="ref19">19</xref>
                </sup>
                <sup>,</sup>
                <sup>
                    <xref ref-type="bibr" rid="ref20">20</xref>
                </sup> Genetic predisposition and hormonal influences could be the reason why depression was higher in females than males. This could also be due to higher reporting of symptoms by females, whereas fewer males may report symptoms due to societal pressure for males to be more emotionally strong.</p>
            <p>Depression was more frequently seen in urban adolescents than rural adolescents in the current study (23.1% and 19.4% respectively). Similar observations were made by Bahl R, and Kumari R in their study, where depression among urban adolescents was about 33.3% and depression among rural adolescents was 20.7%.
                <sup>
                    <xref ref-type="bibr" rid="ref21">21</xref>
                </sup> This could be due to higher exposure of adolescents to risk factors like academic competition and failure, anxiety, and substance abuse, etc.</p>
            <p>Anxiety among adolescents in the current study was found to be 29.2% and 24% in urban and rural areas of Shivamogga respectively. On average, anxiety was found to be 26.6%. This was similar to the study conducted by Kumar K S et al. and Madasu S et al., who found anxiety among adolescents to be 24.4% and 22.7% respectively.
                <sup>
                    <xref ref-type="bibr" rid="ref16">16</xref>
                </sup>
                <sup>,</sup>
                <sup>
                    <xref ref-type="bibr" rid="ref22">22</xref>
                </sup> These studies also found that the prevalence of anxiety was higher among females when compared to males. Similar findings were seen in our study in both urban and rural areas. The prevalence of anxiety was significantly higher among females than males in urban areas. Higher anxiety among females could be due to higher exposure to risk factors like academic competition and societal pressure on women to work and prove themselves as equals to males in the modern world.</p>
            <p>Anxiety was more frequently seen in urban adolescents than rural adolescents in our study (29.4% and 24% respectively). Similar results were observed by Vs P et al.
                <sup>
                    <xref ref-type="bibr" rid="ref23">23</xref>
                </sup> This could be due to more academic competitiveness in an urban area than in a rural area. While the parental expectation and societal pressure to perform better academically and excel in the future and have a well-paying job is higher for males than females, in the modern world the same pressure is higher in females as well in urban areas. This could be the reason why anxiety was more prevalent among urban females than males. As this study was conducted during the pandemic, the increased awareness of COVID in urban areas with better education could also be the reason why anxiety was higher among urban adolescents.</p>
            <p>Stress among the adolescents in our study was found to be 26.6% and 21.1% in urban and rural areas respectively. On average 23.85% of the adolescents were stressed in Shivamogga. Sahoo and Saddicha, in their study, also found that the prevalence of stress among adolescents was 20%.
                <sup>
                    <xref ref-type="bibr" rid="ref18">18</xref>
                </sup> Prevalence of stress was found to be higher in urban areas compared to rural areas. Similar observations were made by Dey BK
                <sup>
                    <xref ref-type="bibr" rid="ref24">24</xref>
                </sup> in Bangladesh. No studies from the Indian setting could be found that compared the prevalence of stress in urban and rural areas.</p>
            <p>Stress was more frequently seen in males than females in both urban and rural areas of Shivamogga. This was contrary to observations made in other studies, where they found that stress was more prevalent among female participants.
                <sup>
                    <xref ref-type="bibr" rid="ref16">16</xref>
                </sup>
                <sup>,</sup>
                <sup>
                    <xref ref-type="bibr" rid="ref24">24</xref>
                </sup> This difference could be due to underreporting of symptoms by female participants. The ongoing pandemic at the time of the study could have contributed to these findings.</p>
            <sec id="sec8">
                <title>Strengths and limitations</title>
                <p>The strength of the study lies in its robust methodology, extensive sample size and comprehensive data analysis. This study employed a well-designed survey or assessment tool to gather data, ensuring the accuracy and reliability of the results. The measurement of depression, anxiety, and stress was conducted using a standardized scale rather than through assessment by a trained psychiatrist.</p>
            </sec>
        </sec>
        <sec id="sec9" sec-type="conclusions">
            <title>Conclusions</title>
            <p>About a quarter of the adolescent population suffers from depression anxiety and stress. It&#x2019;s crucial to detect these symptoms among adolescents and take proper action to avoid compromising their education and development. Those detected to have these symptoms must be referred to be seen by a psychiatrist for clinical evaluation. Further studies are necessary to learn about the causative factors of these mental health disorders. Adopting and implementing better education and health policies are necessary to enhance adolescent mental health.</p>
        </sec>
    </body>
    <back>
        <sec id="sec13" sec-type="data-availability">
            <title>Data availability</title>
            <sec id="sec14">
                <title>Underlying data</title>
                <p>figshare: DASS.xlsx. 
                    <ext-link ext-link-type="uri" xlink:href="https://doi.org/10.6084/m9.figshare.23652477.v2">https://doi.org/10.6084/m9.figshare.23652477.v2</ext-link>

                    <sup>

                        <xref ref-type="bibr" rid="ref25">25</xref>
</sup>
                </p>
                <p>This project contains the underlying data file.</p>
            </sec>
            <sec id="sec15">
                <title>Extended data</title>
                <p>figshare: Questionnaire - Depression, anxiety, and stress among adolescents in Shivamogga, Karnataka. 
                    <ext-link ext-link-type="uri" xlink:href="https://doi.org/10.6084/m9.figshare.23996670.v2">https://doi.org/10.6084/m9.figshare.23996670.v2</ext-link>.
                    <sup>

                        <xref ref-type="bibr" rid="ref26">26</xref>
</sup>
                </p>
                <p>This project contains the blank questionnaire.</p>
                <p>Data are available under the terms of the 
                    <ext-link ext-link-type="uri" xlink:href="https://creativecommons.org/licenses/by/4.0/">Creative Commons Attribution 4.0 International license</ext-link> (CC-BY 4.0).</p>
            </sec>
        </sec>
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    <sub-article article-type="reviewer-report" id="report257925">
        <front-stub>
            <article-id pub-id-type="doi">10.5256/f1000research.152891.r257925</article-id>
            <title-group>
                <article-title>Reviewer response for version 1</article-title>
            </title-group>
            <contrib-group>
                <contrib contrib-type="author">
                    <name>
                        <surname>Saha</surname>
                        <given-names>Indranil</given-names>
                    </name>
                    <xref ref-type="aff" rid="r257925a1">1</xref>
                    <role>Referee</role>
                    <uri content-type="orcid">https://orcid.org/0000-0001-6622-7695</uri>
                </contrib>
                <aff id="r257925a1">
                    <label>1</label>ICMR-Centre for Ageing and Mental Health (I-CAM), Indian Council of Medical Research, Kolkata, West Bengal, India</aff>
            </contrib-group>
            <author-notes>
                <fn fn-type="conflict">
                    <p>
                        <bold>Competing interests: </bold>No competing interests were disclosed.</p>
                </fn>
            </author-notes>
            <pub-date pub-type="epub">
                <day>23</day>
                <month>3</month>
                <year>2024</year>
            </pub-date>
            <permissions>
                <copyright-statement>Copyright: &#x00a9; 2024 Saha I</copyright-statement>
                <copyright-year>2024</copyright-year>
                <license xlink:href="https://creativecommons.org/licenses/by/4.0/">
                    <license-p>This is an open access peer review report distributed under the terms of the Creative Commons Attribution Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.</license-p>
                </license>
            </permissions>
            <related-article ext-link-type="doi" id="relatedArticleReport257925" related-article-type="peer-reviewed-article" xlink:href="10.12688/f1000research.139603.1"/>
            <custom-meta-group>
                <custom-meta>
                    <meta-name>recommendation</meta-name>
                    <meta-value>approve-with-reservations</meta-value>
                </custom-meta>
            </custom-meta-group>
        </front-stub>
        <body>
            <p>The manuscript needs following clarifications: 
                <list list-type="order">
                    <list-item>
                        <p>&#x201c;p-values less than 0.05 were considered significant&#x201d; &#x2013; please correct as p value equal to or less than 0.05 was considered as statistically significant</p>
                    </list-item>
                    <list-item>
                        <p>As per rule the adolescents with completed 19 years should have been included as study participants</p>
                    </list-item>
                    <list-item>
                        <p>Colleges situated in urban and rural area: whether urban college truly represent urban adolescents? There may be so may students from rural area studying in urban college and the vice versa may be true. &#x00a0;</p>
                    </list-item>
                    <list-item>
                        <p>How socioeconomic status was measured in both the colleges?</p>
                    </list-item>
                    <list-item>
                        <p>Please edit the sample size formula &#x2013; &#x201c;n=4pq/D2&#x201d; instead of 4, please mention as Z
                            <sub>&#x221e;</sub>
                            <sup>2</sup>.</p>
                    </list-item>
                    <list-item>
                        <p>In which language this DASS 21 scale was used? Was it validated and reliable in that settings?</p>
                    </list-item>
                </list>
            </p>
            <p>Is the work clearly and accurately presented and does it cite the current literature?</p>
            <p>Yes</p>
            <p>If applicable, is the statistical analysis and its interpretation appropriate?</p>
            <p>Yes</p>
            <p>Are all the source data underlying the results available to ensure full reproducibility?</p>
            <p>Yes</p>
            <p>Is the study design appropriate and is the work technically sound?</p>
            <p>Yes</p>
            <p>Are the conclusions drawn adequately supported by the results?</p>
            <p>Yes</p>
            <p>Are sufficient details of methods and analysis provided to allow replication by others?</p>
            <p>Partly</p>
            <p>Reviewer Expertise:</p>
            <p>Epidemiology, Non-communicable disease, Mental health</p>
            <p>I confirm that I have read this submission and believe that I have an appropriate level of expertise to confirm that it is of an acceptable scientific standard, however I have significant reservations, as outlined above.</p>
        </body>
        <sub-article article-type="response" id="comment11396-257925">
            <front-stub>
                <contrib-group>
                    <contrib contrib-type="author">
                        <name>
                            <surname>Mallya</surname>
                            <given-names>Ajay</given-names>
                        </name>
                        <aff>Community Medicine, Kasturba Medical College, Mangalore, karnataka, India</aff>
                    </contrib>
                </contrib-group>
                <author-notes>
                    <fn fn-type="conflict">
                        <p>
                            <bold>Competing interests: </bold>No competing interests were disclosed.</p>
                    </fn>
                </author-notes>
                <pub-date pub-type="epub">
                    <day>9</day>
                    <month>4</month>
                    <year>2024</year>
                </pub-date>
            </front-stub>
            <body>
                <p>
                    <list list-type="bullet">
                        <list-item>
                            <p>Comment:&#x00a0;&#x201c;p-values less than 0.05 were considered significant&#x201d; &#x2013; please correct as p value equal to or less than 0.05 was considered as statistically significant</p>
                        </list-item>
                    </list> Author&#x2019;s Response:&#x00a0;We agree with your comment. The suggested correction has been made.</p>
                <p> Action Taken in the manuscript: The suggested correction has been made.</p>
                <p> &#x00a0; 
                    <list list-type="bullet">
                        <list-item>
                            <p>Comment:&#x00a0;As per rule the adolescents with completed 19 years should have been included as study participants</p>
                        </list-item>
                    </list> Author&#x2019;s Response:&#x00a0;We agree with your comment. We would like to clarify that adolescents who have completed 19 years were included in the study.</p>
                <p> Action Taken in the manuscript: we have mentioned that adolescents aged 19 years have included in the study in the methodology section.</p>
                <p> &#x00a0; 
                    <list list-type="bullet">
                        <list-item>
                            <p>Comment:&#x00a0;Colleges situated in urban and rural area: whether urban college truly represent urban adolescents? There may be so may students from rural area studying in urban college and the vice versa may be true.</p>
                        </list-item>
                    </list> Author&#x2019;s Response:&#x00a0; In the data collection process, we ensured that the collected data is properly segregated into urban and rural categories based on the list of colleges obtained from the block education officer. However, we acknowledge that some participants may commute and study in a college that does not accurately represent the demography (urban and rural). This can be seen as a limitation of the study.</p>
                <p> Action Taken in the manuscript: Necessary changes have been done in the strengths and limitation section.</p>
                <p> &#x00a0; 
                    <list list-type="bullet">
                        <list-item>
                            <p>Comment:&#x00a0;How socioeconomic status was measured in both the colleges?</p>
                        </list-item>
                    </list> Author&#x2019;s Response:&#x00a0; We have not measured the socioeconomic status of the participants in the study.</p>
                <p> Action Taken in the manuscript: As socioeconomic status was not included in the original study instrument we have not included it in the study.</p>
                <p> &#x00a0; 
                    <list list-type="bullet">
                        <list-item>
                            <p>Comment:&#x00a0;Please edit the sample size formula &#x2013; &#x201c;n=4pq/D2&#x201d; instead of 4, please mention as Z&#x221e;
                                <sup>2</sup>
                            </p>
                        </list-item>
                    </list> Author&#x2019;s Response:&#x00a0;We agree with your observation.</p>
                <p> Action Taken in the manuscript: Necessary changes have been done in the manuscript.</p>
                <p> &#x00a0; 
                    <list list-type="bullet">
                        <list-item>
                            <p>Comment: In which language this DASS 21 scale was used? Was it validated and reliable in that settings?</p>
                        </list-item>
                    </list> Author&#x2019;s Response:&#x00a0;DASS21 Scale was used in English in our study without any translation or alteration. As the students studying in PU college have English as their first language we used the scale without any translations. DASS21 scale in English is already validated among adolescents.</p>
                <p> Action Taken in the manuscript: We have duly incorporated this information into the methodology section of the manuscript for clarity.</p>
            </body>
        </sub-article>
    </sub-article>
</article>
