<?xml version="1.0" encoding="UTF-8"?><!DOCTYPE article PUBLIC "-//NLM//DTD JATS (Z39.96) Journal Publishing DTD v1.2 20190208//EN" "http://jats.nlm.nih.gov/publishing/1.2/JATS-journalpublishing1.dtd"><article xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" article-type="other" dtd-version="1.2" xml:lang="en">
    <front>
        <journal-meta>
            <journal-id journal-id-type="pmc">F1000Research</journal-id>
            <journal-title-group>
                <journal-title>F1000Research</journal-title>
            </journal-title-group>
            <issn pub-type="epub">2046-1402</issn>
            <publisher>
                <publisher-name>F1000 Research Limited</publisher-name>
                <publisher-loc>London, UK</publisher-loc>
            </publisher>
        </journal-meta>
        <article-meta>
            <article-id pub-id-type="doi">10.12688/f1000research.146475.1</article-id>
            <article-categories>
                <subj-group subj-group-type="heading">
                    <subject>Study Protocol</subject>
                </subj-group>
                <subj-group>
                    <subject>Articles</subject>
                </subj-group>
            </article-categories>
            <title-group>
                <article-title>A Cross-sectional study to assess the mental health problems during menstrual cycle among adolescent girls in the rural area of Wardha District</article-title>
                <fn-group content-type="pub-status">
                    <fn>
                        <p>[version 1; peer review: awaiting peer review]</p>
                    </fn>
                </fn-group>
            </title-group>
            <contrib-group>
                <contrib contrib-type="author" corresp="yes">
                    <name>
                        <surname>Bhalerao</surname>
                        <given-names>Vijay</given-names>
                    </name>
                    <role content-type="http://credit.niso.org/">Conceptualization</role>
                    <role content-type="http://credit.niso.org/">Formal Analysis</role>
                    <role content-type="http://credit.niso.org/">Visualization</role>
                    <role content-type="http://credit.niso.org/">Writing &#x2013; Review &amp; Editing</role>
                    <xref ref-type="corresp" rid="c1">a</xref>
                    <xref ref-type="aff" rid="a1">1</xref>
                </contrib>
                <contrib contrib-type="author" corresp="no">
                    <name>
                        <surname>Gotarkar</surname>
                        <given-names>Shashank</given-names>
                    </name>
                    <role content-type="http://credit.niso.org/">Supervision</role>
                    <role content-type="http://credit.niso.org/">Validation</role>
                    <role content-type="http://credit.niso.org/">Writing &#x2013; Original Draft Preparation</role>
                    <xref ref-type="aff" rid="a2">2</xref>
                </contrib>
                <contrib contrib-type="author" corresp="no">
                    <name>
                        <surname>Muneshwar</surname>
                        <given-names>Komal</given-names>
                    </name>
                    <role content-type="http://credit.niso.org/">Supervision</role>
                    <role content-type="http://credit.niso.org/">Validation</role>
                    <role content-type="http://credit.niso.org/">Writing &#x2013; Original Draft Preparation</role>
                    <xref ref-type="aff" rid="a2">2</xref>
                </contrib>
                <contrib contrib-type="author" corresp="no">
                    <name>
                        <surname>Vaishnav</surname>
                        <given-names>Dr Lokesh</given-names>
                    </name>
                    <role content-type="http://credit.niso.org/">Supervision</role>
                    <role content-type="http://credit.niso.org/">Visualization</role>
                    <role content-type="http://credit.niso.org/">Writing &#x2013; Review &amp; Editing</role>
                    <uri content-type="orcid">https://orcid.org/0000-0002-6091-5275</uri>
                    <xref ref-type="aff" rid="a3">3</xref>
                </contrib>
                <aff id="a1">
                    <label>1</label>School of Epidemiology and Public Health, Datta Meghe Institute of Higher Education and Research, Wardha, Maharashtra, 442001, India, Wardha, Maharastra, 442001, India</aff>
                <aff id="a2">
                    <label>2</label>Assistant Professor, Department of Community Medicine and Public Health, Datta Meghe Institute of Higher Education and Research, Wardha, Maharashtra, 442001, India</aff>
                <aff id="a3">
                    <label>3</label>Senior Resident, Department of Community Medicine and Public Health, Datta Meghe Institute of Higher Education and Research, Wardha, Maharashtra, 442001, India</aff>
            </contrib-group>
            <author-notes>
                <corresp id="c1">
                    <label>a</label>
                    <email xlink:href="mailto:vijay.bhalerao@dmiher.edu.in">vijay.bhalerao@dmiher.edu.in</email>
                </corresp>
                <fn fn-type="conflict">
                    <p>No competing interests were disclosed.</p>
                </fn>
            </author-notes>
            <pub-date pub-type="epub">
                <day>2</day>
                <month>5</month>
                <year>2024</year>
            </pub-date>
            <pub-date pub-type="collection">
                <year>2024</year>
            </pub-date>
            <volume>13</volume>
            <elocation-id>436</elocation-id>
            <history>
                <date date-type="accepted">
                    <day>19</day>
                    <month>3</month>
                    <year>2024</year>
                </date>
            </history>
            <permissions>
                <copyright-statement>Copyright: &#x00a9; 2024 Bhalerao V et al.</copyright-statement>
                <copyright-year>2024</copyright-year>
                <license xlink:href="https://creativecommons.org/licenses/by/4.0/">
                    <license-p>This is an open access article distributed under the terms of the Creative Commons Attribution Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.</license-p>
                </license>
            </permissions>
            <self-uri content-type="pdf" xlink:href="https://f1000research.com/articles/13-436/pdf"/>
            <abstract>
                <sec>
                    <title>Introduction</title>
                    <p>It is essential to improve global public health and to achieve sustainable developmental goals. Mental health problems are common during menstruation in adolescent girls. Menstrual problems affect academic performance, personal lives, and social lives. Menstrual problems are both affective and somatic.</p>
                    <p>This study is undertaken to study the Impact of Menstruation on the Mental Health of young adolescent girls. The participants &#x2018; ages range from 15 to 19 years. Adolescent coping method ds Mental health, psychological well-being, and spiritual well-being were assessed. Menstrual-phase respondents exhibited increased psychological traits as a measure of adolescent girls&#x2019; coping with mental difficulties.</p>
                </sec>
                <sec>
                    <title>Objectives</title>
                    <p>To evaluate menstruation-related knowledge, attitude, sources of information, and psychological well-being</p>
                    <p>To determine the mental health problems during the menstrual cycle.</p>
                    <p>To find out the association between sociodemographic variables and mental health problems during menstrual cycle</p>
                </sec>
                <sec>
                    <title>Methods</title>
                    <p>A cross-sectional study was conducted in a rural area of the Wardha district. This study used a descriptive cross-sectional research design. A sample of 364 individuals was selected using random sampling. The inclusion criteria were adolescent girls aged 15&#x2013;19 years who attained menstruation. Exclusion criteria: Girls younger than 15 years and above 19 years, as well as those who were hesitant and uncomfortable asking for information to participate in the study, were excluded.</p>
                </sec>
                <sec>
                    <title>Outcomes</title>
                    <p>Adolescent girls experienced menstruation-related psychosocial issues that are not uncommon. Menstrual distress, burden, stigma, lack of counselling services, lack of preparation menarche, lack of easy access to clean resources, vacy concerns when changing pads are necessary, lack of facilities for disposing sanitary napkins, and inadequate psychological and social support. Most girls experienced stress, mood swings, and restlessness during their menstrual cycle. These findings are consistent with past studies that found that the majority of girls were agitated during menstruation. Approximately 25% of them felt ashamed and guilty about their pubertal changes, and experienced menarche in fear, shyness, and sadness. On the first day of menstruation, girls report psychological problems such as anxiety, trouble concentrating, sadness, irritability, and excitement7. Approximately two-thirds of teenagers in the current study were unaware that menarche occurred earlier.</p>
                </sec>
            </abstract>
            <kwd-group kwd-group-type="author">
                <kwd>Adolescent girls</kwd>
                <kwd>menstrual problems</kwd>
                <kwd>mental health</kwd>
                <kwd>lifestyle</kwd>
            </kwd-group>
            <funding-group>
                <funding-statement>The author(s) declared that no grants were involved in supporting this work.</funding-statement>
            </funding-group>
        </article-meta>
    </front>
    <body>
        <sec id="sec5" sec-type="intro">
            <title>Introduction</title>
            <p>Adolescent girls frequently experience menstrual problems. The three most prevalent menstruation disorders in teenagers are dysfunctional uterine bleeding and amenorrhea, according to Gueydan and McAnamey (1982), who reviewed several studies on this subject.
                <sup>
                    <xref ref-type="bibr" rid="ref1">1</xref>
                </sup> The Latin term &#x201c;Adolescere,&#x201d; which means &#x201c;to grow in maturity,&#x201d; is the source of the English word &#x201c;adolescence. Adolescents between the ages of 10 and 19 years were considered adolescents. In addition to physical growth and maturity, adolescence is a time of cerebral and emotional growth.
                <sup>
                    <xref ref-type="bibr" rid="ref2">2</xref>
                </sup> According to McEvoy et al. (2004), menstrual problems such as amenorrhea, heavy vaginal bleeding, menstrual cramps, and menstrual syndrome are typical causes for teenage females to consult a doctor.
                <sup>
                    <xref ref-type="bibr" rid="ref1">1</xref>
                </sup>
            </p>
            <p>In India, only 30% of healthcare services are available in rural areas, while 70% are available in metropolitan areas. Most teenage girls and young women do not seek healthcare services because of ignorance, lack of education, cultural taboos, and male domination. At the same time, the high incidence of nutritional deficiency among adolescent girls causes more significant reproductive issues at a young age. Menstrual issues may affect 75% of girls and are the main reason for frequent absences in school.
                <sup>
                    <xref ref-type="bibr" rid="ref3">3</xref>
                </sup> Teenagers frequently relapsed into psychiatric illnesses during the study period. This is because they do not realize the problems that might arise during menstruation or how to address them. Nearly 25% of teenagers struggle with psychological issues including isolated physical health issues. The statement mentioned above demonstrates how crucial it is for students or teenagers to understand how psychological issues might return during menstruation.
                <sup>
                    <xref ref-type="bibr" rid="ref4">4</xref>
                </sup>
            </p>
            <p>To our knowledge, little research has been conducted on how these characteristics relate to menstrual symptoms among teenage girls. When determining the etiology of menstrual problems, examining the relationship between them and menstrual symptoms is critical, because menarche occurs later in puberty. During pubertal years, there are increases in depressive symptoms, anxiety, and smoking behavior. The main goals of this study were to: a) describe differences in self-reported menstrual symptoms in adolescents regarding smoking behavior. b) Examine the relationship between depressive symptoms, anxiety, and menstrual symptoms. c) To examine whether smoking moderates this relationship.
                <sup>
                    <xref ref-type="bibr" rid="ref5">5</xref>
                </sup> Age, ethnicity, family history, smoking, physical activity, and nutritional choices are only a few variables that might impact menstrual cycles.
                <sup>
                    <xref ref-type="bibr" rid="ref15">6</xref>
                </sup> Menstrual abnormalities can be significantly exacerbated or caused by stress. There is evidence linking stress to</p>
            <p>There are several irregularities, including menorrhagia, oligomenorrhea, dysmenorrhea, and PMS.
                <sup>
                    <xref ref-type="bibr" rid="ref6">7</xref>
                </sup> Menstrual abnormalities can be significantly exacerbated or caused by stress, and there is evidence linking stress to several irregularities, including menorrhagia, oligomenorrhea, dysmenorrhea, and PMS.
                <sup>
                    <xref ref-type="bibr" rid="ref6">7</xref>
                </sup> The early detection of the causes of typical teen menstruation patterns may aid in their prevention, improve adolescent girls&#x2019; reproductive health, and enhance their standard of life.
                <sup>
                    <xref ref-type="bibr" rid="ref7">8</xref>
                </sup> In every menstrual cycle, females of reproductive age experience dramatic changes in the levels of sex hormones (such as progesterone and estrogen). According to the points mentioned above, a contradiction also appears that suggests that menstruation has favorable effects on adolescent girls.
                <sup>
                    <xref ref-type="bibr" rid="ref8">9</xref>
                </sup>
                <sup>,</sup>
                <sup>
                    <xref ref-type="bibr" rid="ref9">10</xref>
                </sup> They are concerning menstruation. People maintain a relatively conservative mentality in a society dominated by cultural and religious constraints. Adolescent girls, on the other hand, typically have unfavorable views and ideas about menstruation due to cultural standards.
                <sup>
                    <xref ref-type="bibr" rid="ref10">11</xref>
                </sup>
                <sup>,</sup>
                <sup>
                    <xref ref-type="bibr" rid="ref11">12</xref>
                </sup> Several factors influence the duration and regularity of the menstrual cycle, including sociodemographic profile, psychological stress, disturbed sleep level, rigorous physical exercise, and food.
                <sup>
                    <xref ref-type="bibr" rid="ref12">13</xref>
                </sup>
            </p>
            <sec id="sec6">
                <title>Rationale</title>
                <p>Rural studies were more numerous among adolescent girls across rural locations than in rural ones. The majority of research has examined awareness, attitude, conception, beliefs, cultural elements, factors, experiences, general well-being, sanitation, seeking support behavior, knowledge, perceptions, practices, prevalence, and menstruation issues. Very few Indian studies have been conducted in this community. Most of these occurred in schools. Although urban research outnumbered rural research, several studies have examined menstruation in adolescent females from rural and urban regions. More research needs to be done on menstruation in non-schooled adolescents and on interventions. Most studies have emphasized menstrual patterns, customs, and issues.
                    <sup>
                        <xref ref-type="bibr" rid="ref15">6</xref>
                    </sup>
                    <sup>,</sup>
                    <sup>
                        <xref ref-type="bibr" rid="ref11">12</xref>
                    </sup>
                    <sup>,</sup>
                    <sup>
                        <xref ref-type="bibr" rid="ref13">14</xref>
                    </sup>
                    <sup>,</sup>
                    <sup>
                        <xref ref-type="bibr" rid="ref14">15</xref>
                    </sup> Most Indian studies were community-based; only a few were conducted in rural areas.</p>
            </sec>
            <sec id="sec7">
                <title>Objectives</title>
                <p>
                    <list list-type="order">
                        <list-item>
                            <label>1.</label>
                            <p>To evaluate menstruation-related knowledge, attitudes, sources of information, and psychological well-being.</p>
                        </list-item>
                        <list-item>
                            <label>2.</label>
                            <p>To determine the mental health problems during Menstrual cycle.</p>
                        </list-item>
                        <list-item>
                            <label>3.</label>
                            <p>To find out the association between sociodemographic variables and mental health problems during menstrual cycle.</p>
                        </list-item>
                    </list>
                </p>
            </sec>
            <sec id="sec8">
                <title>Aim</title>
                <p>To determine the association between menstrual cycles of adolescent girls and their mental health in central rural India.</p>
            </sec>
        </sec>
        <sec id="sec9" sec-type="methods">
            <title>Methods</title>
            <sec id="sec10">
                <title>Study design</title>
                <p>The present study will be a cross-sectional study.</p>
            </sec>
            <sec id="sec11">
                <title>Study setting/place</title>
                <p>
                    <bold>Eligibility criteria</bold>
                </p>
                <p>The inclusion criteria were adolescent girls aged 15&#x2013;19 years who attained menstruation. Exclusion criteria: Girls younger than 15 years and above 19 years, as well as those who were hesitant and uncomfortable asking for information to participate in the study, were excluded.</p>
                <p>The pre-validated questionnaire will be used to collect data in the rural part of the Wardha district in Central India.</p>
            </sec>
            <sec id="sec12">
                <title>Study participants/population</title>
                <p>This study will be conducted among adolescent girls aged 15-19 in the rural area of the Wardha district. Google and paper-based forms will be used to contact individuals and invite them to participate in the study. They will be given access to the online and offline questionnaires. The data tool will be in print format and will be available in Marathi, English, and Hindi.</p>
                <p>This study will be conducted among adolescent girls aged 15-19 in the rural area of the Wardha district.</p>
            </sec>
            <sec id="sec13">
                <title>Variables</title>
                <p>This study examined variables related to menstruation problems, knowledge, attitude, and practice. Age at menarche, days of the cycle: The individual&#x2019;s stress, worry, and sadness levels rose when menstrual symptoms were more intense. The duration, length, and amount of menstrual bleeding did not significantly correlate with menstrual symptoms such as anxiety, depression, or stress, according to the Pearson correlation test. Menstruation-related distress (MEID-Q) and the Hamilton Depression Rating Test (HDRS). Percentage of mental health among adolescent girls living in rural areas.</p>
                <table-wrap id="T1" orientation="portrait" position="anchor">
                    <label>Table 1. </label>
                    <caption>
                        <title>The key parameters, including their variables, data sources, and methods of data collection, are essential components to be identified.</title>
                    </caption>
                    <table content-type="article-table" frame="hsides">
                        <thead>
                            <tr>
                                <th align="left" colspan="1" rowspan="1" valign="top">Key parameters</th>
                                <th align="left" colspan="1" rowspan="1" valign="top">Variable&#x2019;s</th>
                                <th align="left" colspan="1" rowspan="1" valign="top">Study participants</th>
                                <th align="left" colspan="1" rowspan="1" valign="top">Data collection methods Data collection methods</th>
                            </tr>
                        </thead>
                        <tbody>
                            <tr>
                                <td align="left" colspan="1" rowspan="1" valign="top">Demographic profile of adolescent girls in rural areas of Wardha.</td>
                                <td align="left" colspan="1" rowspan="1" valign="top">Age
                                    <break/>Education
                                    <break/>Type of family
                                    <break/>Marital status
                                    <break/>Life style
                                    <break/>Socioeconomic status
                                    <break/>
                                    <styled-content style="#212121" style-type="color">Unstable &amp; moody before periods Headache before periods</styled-content>
                                </td>
                                <td align="left" colspan="1" rowspan="1" valign="top">Adolescents girls (15-19) age</td>
                                <td align="left" colspan="1" rowspan="1" valign="top">A survey using method offline and online questionnaires.</td>
                            </tr>
                            <tr>
                                <td align="left" colspan="1" rowspan="1" valign="top">Assessment of mental health problems amongst adolescent girls</td>
                                <td align="left" colspan="1" rowspan="1" valign="top">levels of stress
                                    <break/>sadness
                                    <break/>anxiety
                                    <break/>depression
                                    <break/>stress
                                    <break/>sleep disturbances</td>
                                <td align="left" colspan="1" rowspan="1" valign="top">Adolescents girls (15-19) age</td>
                                <td align="left" colspan="1" rowspan="1" valign="top">A survey using method offline and online questionnaires.</td>
                            </tr>
                            <tr>
                                <td align="left" colspan="1" rowspan="1" valign="top">Assessment of menstrual-related factors</td>
                                <td align="left" colspan="1" rowspan="1" valign="top">Discomfort due to vaginal bleeding.
                                    <break/>the feeling of being dirty
                                    <break/>difficulty concentrating
                                    <break/>Interfered with your quality of life.
                                    <break/>discomfort from the tampon
                                    <break/>Reactions during the first menstrual cycle</td>
                                <td align="left" colspan="1" rowspan="1" valign="top">Adolescents girls (15-19) age</td>
                                <td align="left" colspan="1" rowspan="1" valign="top">A survey using method offline and online questionnaires.</td>
                            </tr>
                        </tbody>
                    </table>
                </table-wrap>
            </sec>
            <sec id="sec14">
                <title>Data collection procedure</title>
                <p>The tool comprises an offline consent form and online questionnaire. The data collection process involved the use of the Kobo collection tool. Each survey ensured anonymity and included a page with permission for information and details about the objectives of the study.</p>
            </sec>
            <sec id="sec15">
                <title>Data collection tools</title>
                <p>The tool consists of an online questionnaire and offline collection of data. The questionnaire was produced using the Kobo collection app and offline forms. The first component of the questionnaire concerned the individual&#x2019;s demographic information. The Menstrual Distress Questionnaire (MEDI-Q) examines and evaluates adolescent girls&#x2019; overall distress throughout their mental health. The MEDI-Q questionnaire tool has been validated among people aged 15 to 19, with established concurrent and criterion validity in many languages and a test-retest reliability of 0.61.
                    <sup>
                        <xref ref-type="bibr" rid="ref17">16</xref>
                    </sup>
                    <sup>,</sup>
                    <sup>
                        <xref ref-type="bibr" rid="ref18">17</xref>
                    </sup>
                </p>
                <p>The following section goes through the scale&#x2019;s item numbering system item by item, using the prefix &#x201c;H&#x201d; for HAM-D items and &#x201c;A&#x201d; for a typical subscale item, followed by the item&#x2019;s name, as it appears on the scale to measure mental depression and mental stress. This item is specific to depressed mood, which, according to our standards, is any feeling of emotion, including those mentioned in the item&#x2019;s label, as well as low, blue, sad, dysphonic, sobbing, etc. Other negative mood states (e.g., anxiety, tension, anger, and irritability).
                    <sup>
                        <xref ref-type="bibr" rid="ref19">18</xref>
                    </sup>
                    <sup>,</sup>
                    <sup>
                        <xref ref-type="bibr" rid="ref20">19</xref>
                    </sup>
                </p>
            </sec>
            <sec id="sec16">
                <title>Sample size</title>
                <p>Menstruation has an impact on mental health, according to a prior study conducted by Field.
                    <sup>
                        <xref ref-type="bibr" rid="ref16">20</xref>
                    </sup>
                </p>
                <p>The sample size was calculated using the following article, and the proportion of sleep disturbance was found to be 61.6%.</p>
                <p>The sample size was calculated using the following equation: Where Alpha (&#x03b1;) is 0.05, proportion (p) is estimated to be 0.61, and the error estimate (d) is 0.05.</p>
                <p>The sample size of this study was 364. The rate of sleep disturbance in adolescent girls living in rural areas was examined using data; therefore, the sample size was 364 adolescent girls.</p>
            </sec>
            <sec id="sec17">
                <title>Sampling method</title>
                <p>We used a simple random sampling method. Adolescent girls will be comfortable sharing study information related to menstruation and mental health effects during menstruation. The adolescent girls did not hesitate to participate in the interviews.</p>
            </sec>
            <sec id="sec18">
                <title>Analysis plan</title>
                <p>The Statistical Package for the Social Sciences SPSS 24.0 software [
                    <ext-link ext-link-type="uri" xlink:href="https://spss.software.informer.com/24.0/">SPSS 24.0 Download - spss.exe (informer.com)</ext-link>] will analyse the data after converting it to an MS Excel spreadsheet. We will calculate descriptive statistics, including the mean, mode, and median, for variables such as age and period. We then used the chi-squared test to analyze the data.</p>
            </sec>
            <sec id="sec19">
                <title>Expected outcomes/result</title>
                <p>Adolescent girls experience menstruation-related psychosocial issues that are not an uncommon sight. Menstrual distress, burden, stigma, lack of counselling services, lack of preparation menarche, lack of easy access to clean resources, privacy concerns when changing pads are necessary, lack of facilities for disposing sanitary napkins, and inadequate psychological and social support. Most girls experienced stress, mood swings, and restlessness during their menstrual cycle. These findings were consistent with those of previous studies have discovered that the majority of girls were agitated during menstruation. Approximately 25% of them felt ashamed and guilty about their pubertal changes, and experienced menarche in fear, shyness, and sadness. On the first day of menstruation, girls report psychological problems such as anxiety, trouble concentrating, sadness, irritability, and excitement.
                    <sup>
                        <xref ref-type="bibr" rid="ref7">8</xref>
                    </sup> Approximately two-thirds of teenagers in the current study were unaware that menarche occurred earlier.</p>
            </sec>
            <sec id="sec20">
                <title>Possible confounders</title>
                <p>When assessing mental health symptoms, several factors may need to be considered, including age, education, marital status, socioeconomic status, lifestyle, baseline symptoms of mental health disorders, frequency of sleep, sadness, anxiety, depression, and stress. Demographic profiles and mental health issues will be used to analyze and assess these variables.</p>
            </sec>
            <sec id="sec21">
                <title>Limitations of the study</title>
                <p>Throughout the study, the researcher approached the community base to conduct research in and around the Wardha district. Consequently, the researcher will be unable to assess the mental health issues faced by adolescent girls in rural and urban communities. No standardized questionnaire will be used in the study to measure their mental health issues.</p>
            </sec>
            <sec id="sec22">
                <title>Implementation</title>
                <p>Individual understanding: Menstruation is a natural psychological process in adolescent girls and how to deal with it. Enabling encouraging menstruation is typical of non-taboos and non-myths, with no restrictions.</p>
                <p>Practice improves psychological health by using materials to absorb and collect menstruation blood prevention and clean facilities to change and dispose of the materials (at home, school, work facility for menstruation hygiene, and pain management demonstration of how to use MHH materials). Improved accessibility of menstrual products. Adolescent girls encounter considerable psychosocial challenges during menstruation, including limited access to clean materials, a lack of privacy for change pads, sanitary napkin disposal facilities, sociocultural constraints, and psychological and social support.</p>
            </sec>
        </sec>
        <sec id="sec23" sec-type="discussion">
            <title>Discussion</title>
            <p>Most females experienced stress, mood swings, and restlessness during their periods. These results were in line with those of other studies, which showed that most females experienced irritation during their periods. Almost one-fourth of them felt ashamed and guilty about their pubertal changes and were terrified, shy, and depressed during menarche.
                <sup>
                    <xref ref-type="bibr" rid="ref13">14</xref>
                </sup> Note that although menstruation occurs in teenagers as young as 10 years old, this condition is not regarded as a disorder or sickness. However, in actuality, this can happen owing to the influence of the child&#x2019;s intrinsic factor, which nutrition and hormonal conditions can impact, and most anxiety is caused by adolescents feeling unprepared.
                <sup>
                    <xref ref-type="bibr" rid="ref4">4</xref>
                </sup>
            </p>
            <p>Different age groups; socioeconomic, cultural, and geographic factors; and diverse accepted methods and instruments may contribute to the variance. According to the study&#x2019;s findings, there is a positive correlation between periods of menstruation symptoms during the premenstrual, menstrual, and postmenstrual periods and the symptoms of anxiety, stress, and depression. As a result, menstrual symptoms increase the severity of depressive disorders, anxiety, and stress. Premenstrual symptoms were positively correlated with sadness, anxiety, and stress disorders, and a study on 18-28-year-old students in Zahedan using a questionnaire with 20 questions reported low back pain, abdominal bloating, and tender breasts as the most prevalent pre-menstruation signs.
                <sup>
                    <xref ref-type="bibr" rid="ref21">21</xref>
                </sup>
            </p>
            <p>This suggests that random variables blame the mean-score disparity. The current findings are consistent with earlier research, which indicated that emotional well-being was lower during the premenstrual period. Unlike previous studies, the results showed that emotional well-being did not improve during the follicular phase.
                <sup>
                    <xref ref-type="bibr" rid="ref8">9</xref>
                </sup>
            </p>
            <p>Low socioeconomic position, regional distribution, psychosocial factors frequently linked to menstruation disorders, inadequate environmental stimuli, poor food, nutritional anemia, and age at menarche were all prevalent in the study population. Most problems are preventable and a positive outlook is required.
                <sup>
                    <xref ref-type="bibr" rid="ref3">3</xref>
                </sup>
            </p>
            <p>Specifically, a higher N170 amplitude was observed in the current study in response to amorous male reactions in the follicular phase. Bias against people of the opposite or same sex might explain this result. Numerous studies have shown that attractive individuals of the opposite sex elicit more powerful and immediate responses than attractive individuals of the same sex. Because of this, women in the follicle stages of their period most strongly replied to the man&#x2019;s friendly face. Since the emotional faces may be more &#x201c;intense&#x201d; or &#x201c;provocative&#x201d; to the brains than the other faces, the variations in N170 amplitude triggered by the emotional expressions in the present study and further investigations may be attributable to this.
                <sup>
                    <xref ref-type="bibr" rid="ref9">10</xref>
                </sup>
            </p>
            <p>What does this image represent?</p>
            <fig fig-type="figure" id="f1" orientation="portrait" position="float">
                <label>Figure 1. </label>
                <caption>
                    <title>Common symptoms of adolescent girls during the menstrual cycle.
                        <sup>
                            <xref ref-type="bibr" rid="ref3">3</xref>
                        </sup>
                        <sup>,</sup>
                        <sup>
                            <xref ref-type="bibr" rid="ref4">4</xref>
                        </sup>
                        <sup>,</sup>
                        <sup>
                            <xref ref-type="bibr" rid="ref8">9</xref>
                        </sup>
                        <sup>,</sup>
                        <sup>
                            <xref ref-type="bibr" rid="ref9">10</xref>
                        </sup>
                        <sup>,</sup>
                        <sup>
                            <xref ref-type="bibr" rid="ref21">21</xref>
                        </sup>
                    </title>
                </caption>
                <graphic id="gr1" orientation="portrait" position="float" xlink:href="https://f1000research-files.f1000.com/manuscripts/160567/d9ef5545-2924-4ef1-ac54-39e9c16c14db_figure1.gif"/>
            </fig>
            <sec id="sec24">
                <title>Ethics and consent</title>
                <p>The Dtta Meghe Institute of Higher Education and Research (DU),Ethical Approval (DMIHER (DU)/IEC/2023/42) was granted by Institutional Ethical committee on Human Research for this study on dated 20/12/2023 additionally, written informed consent will be obtained from all participants before their inclusion in the study. Privacy and confidentially will be maintained throughout the study. Measures will be taken to minimize any potential harm or risks to the participant&#x2019;s such as referral for medical consultation if needed.</p>
            </sec>
            <sec id="sec25">
                <title>Study status</title>
                <p>The study has not yet started; only data collection procedure started.</p>
            </sec>
        </sec>
    </body>
    <back>
        <sec id="sec28">
            <title>Data availability statement</title>
            <sec id="sec29">
                <title>Underlying data</title>
                <p>No data is associated with this article.</p>
            </sec>
            <sec id="sec30">
                <title>Reporting guidelines</title>
                <p>File name STROBE checklist for &#x201c;A cross-sectional study to assess the mental health problems during menstruation cycle among adolescent girls in the area of Wardha district.&#x201d;</p>
                <p>Repository name: figshare DOI: 
                    <ext-link ext-link-type="uri" xlink:href="https://doi.org/10.6084/m9.figshare.25378363">10.6084/m9.figshare.25378363</ext-link>.</p>
                <p>License: 
                    <ext-link ext-link-type="uri" xlink:href="https://creativecommons.org/licenses/by/4.0/legalcode">CC BY 4.0</ext-link>.</p>
            </sec>
        </sec>
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    </back>
</article>
