<?xml version="1.0" encoding="UTF-8"?><!DOCTYPE article PUBLIC "-//NLM//DTD JATS (Z39.96) Journal Publishing DTD v1.2 20190208//EN" "http://jats.nlm.nih.gov/publishing/1.2/JATS-journalpublishing1.dtd"><article xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" article-type="research-article" dtd-version="1.2" xml:lang="en">
    <front>
        <journal-meta>
            <journal-id journal-id-type="pmc">F1000Research</journal-id>
            <journal-title-group>
                <journal-title>F1000Research</journal-title>
            </journal-title-group>
            <issn pub-type="epub">2046-1402</issn>
            <publisher>
                <publisher-name>F1000 Research Limited</publisher-name>
                <publisher-loc>London, UK</publisher-loc>
            </publisher>
        </journal-meta>
        <article-meta>
            <article-id pub-id-type="doi">10.12688/f1000research.147840.2</article-id>
            <article-categories>
                <subj-group subj-group-type="heading">
                    <subject>Research Article</subject>
                </subj-group>
                <subj-group>
                    <subject>Articles</subject>
                </subj-group>
            </article-categories>
            <title-group>
                <article-title>Effect of Informative Cesarean Delivery Operative Steps Video on Maternal Anxiety Level: A Randomized Controlled Trial</article-title>
                <fn-group content-type="pub-status">
                    <fn>
                        <p>[version 2; peer review: 1 approved, 1 approved with reservations]</p>
                    </fn>
                </fn-group>
            </title-group>
            <contrib-group>
                <contrib contrib-type="author" corresp="no">
                    <name>
                        <surname>Phetprapasri</surname>
                        <given-names>Panicha</given-names>
                    </name>
                    <role content-type="http://credit.niso.org/">Conceptualization</role>
                    <role content-type="http://credit.niso.org/">Data Curation</role>
                    <uri content-type="orcid">https://orcid.org/0000-0002-3394-8397</uri>
                    <xref ref-type="aff" rid="a1">1</xref>
                </contrib>
                <contrib contrib-type="author" corresp="yes">
                    <name>
                        <surname>Chanthasenanont</surname>
                        <given-names>Athita</given-names>
                    </name>
                    <role content-type="http://credit.niso.org/">Supervision</role>
                    <role content-type="http://credit.niso.org/">Validation</role>
                    <xref ref-type="corresp" rid="c1">a</xref>
                    <xref ref-type="aff" rid="a1">1</xref>
                </contrib>
                <contrib contrib-type="author" corresp="no">
                    <name>
                        <surname>Prasongvej</surname>
                        <given-names>Pichita</given-names>
                    </name>
                    <role content-type="http://credit.niso.org/">Formal Analysis</role>
                    <xref ref-type="aff" rid="a1">1</xref>
                </contrib>
                <contrib contrib-type="author" corresp="no">
                    <name>
                        <surname>Nuallaong</surname>
                        <given-names>Winitra</given-names>
                    </name>
                    <role content-type="http://credit.niso.org/">Methodology</role>
                    <xref ref-type="aff" rid="a2">2</xref>
                </contrib>
                <contrib contrib-type="author" corresp="no">
                    <name>
                        <surname>Chaitrakulthong</surname>
                        <given-names>Suphachai</given-names>
                    </name>
                    <role content-type="http://credit.niso.org/">Visualization</role>
                    <xref ref-type="aff" rid="a3">3</xref>
                </contrib>
                <contrib contrib-type="author" corresp="no">
                    <name>
                        <surname>Pongrojpaw</surname>
                        <given-names>Densak</given-names>
                    </name>
                    <role content-type="http://credit.niso.org/">Writing &#x2013; Review &amp; Editing</role>
                    <uri content-type="orcid">https://orcid.org/0000-0002-8315-0447</uri>
                    <xref ref-type="aff" rid="a1">1</xref>
                </contrib>
                <aff id="a1">
                    <label>1</label>Department of Obstetrics and Gynecology, Faculty of Medicine, Thammasat University, Khlong Luang, Pathum Thani, 12120, Thailand</aff>
                <aff id="a2">
                    <label>2</label>Department of Psychiatry, Faculty of Medicine, Thammasat University, Khlong Luang, Pathum Thani, 12120, Thailand</aff>
                <aff id="a3">
                    <label>3</label>Thammasat University Hospital, Thammasat University, Khlong Luang, Pathum Thani, 12120, Thailand</aff>
            </contrib-group>
            <author-notes>
                <corresp id="c1">
                    <label>a</label>
                    <email xlink:href="mailto:Cathita@tu.ac.th">Cathita@tu.ac.th</email>
                </corresp>
                <fn fn-type="conflict">
                    <p>No competing interests were disclosed.</p>
                </fn>
            </author-notes>
            <pub-date pub-type="epub">
                <day>16</day>
                <month>9</month>
                <year>2024</year>
            </pub-date>
            <pub-date pub-type="collection">
                <year>2024</year>
            </pub-date>
            <volume>13</volume>
            <elocation-id>712</elocation-id>
            <history>
                <date date-type="accepted">
                    <day>10</day>
                    <month>9</month>
                    <year>2024</year>
                </date>
            </history>
            <permissions>
                <copyright-statement>Copyright: &#x00a9; 2024 Phetprapasri P et al.</copyright-statement>
                <copyright-year>2024</copyright-year>
                <license xlink:href="https://creativecommons.org/licenses/by/4.0/">
                    <license-p>This is an open access article distributed under the terms of the Creative Commons Attribution Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.</license-p>
                </license>
            </permissions>
            <self-uri content-type="pdf" xlink:href="https://f1000research.com/articles/13-712/pdf"/>
            <abstract>
                <sec>
                    <title>Background</title>
                    <p>Cesarean section is the most common obstetric procedure performed. This can lead to maternal anxiety, which is a significant contributor to postpartum depression. This can adversely affect pregnant women both mentally and emotionally, negatively impacting their well-being and family bonding. The aim of this study was to evaluate the effect of the addition of an informative cesarean section operative steps video on the maternal anxiety score compared with standard pre-cesarean section care.</p>
                </sec>
                <sec>
                    <title>Methods</title>
                    <p>This randomized controlled trial was conducted at Thammasat University Hospital, Thailand, between April and September 2023. Pregnant women who underwent their first cesarean section were allocated to two groups: intervention and control groups. Participants in the intervention group were required to watch a 5-minute informative video that elaborately described the process from pre-operative steps until post-operative care on the day scheduled for cesarean delivery. All participants received the same routine pre-operative and post-operative care. The State-Trait Anxiety Inventory (STAI) was used to measure both populations on two occasions: the day of scheduled cesarean delivery and postpartum day 1.</p>
                </sec>
                <sec>
                    <title>Results</title>
                    <p>A total of 178 women were recruited. The demographic and obstetric characteristics were similar between the two groups. The pre-operative STAI scores of the intervention and control groups were 42.9 and 44.1 points, respectively, with no significant difference. However, the post-operative anxiety score showed a significant decline in the intervention group compared to that in the control group (p = 0.002). Moreover, most of the participants in the intervention group showed a low level of anxiety after the operation, while half of the control group remained at a moderate to high level score.</p>
                </sec>
                <sec>
                    <title>Conclusions</title>
                    <p>The provision of an informative educational video before cesarean delivery is a powerful tool that significantly reduces cesarean operative anxiety and improves health outcomes.</p>
                    <p>Thai Clinical Trials Registry on the 28 March 2023 (
                        <uri xlink:href="https://www.thaiclinicaltrials.org/show/TCTR20230328001">TCTR20230328001</uri>).</p>
                </sec>
            </abstract>
            <kwd-group kwd-group-type="author">
                <kwd>Cesarean section</kwd>
                <kwd>Anxiety</kwd>
                <kwd>Informative video.</kwd>
            </kwd-group>
            <funding-group>
                <award-group id="fund-1">
                    <funding-source>Research Fund of Thammasat University, Pathum Thani, Thailand</funding-source>
                </award-group>
                <funding-statement>             This trial was funded by the Research Fund of Thammasat University, Pathum Thani, Thailand in 2023.</funding-statement>
                <funding-statement>
                    <italic>The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.</italic>
                </funding-statement>
            </funding-group>
        </article-meta>
        <notes>
            <sec sec-type="version-changes">
                <label>Revised</label>
                <title>Amendments from Version 1</title>
                <p>The three references are provided to underscore the significance of monitoring postnatal anxiety and related factors, as recommended by the reviewer. Furthermore, we elucidate the research methodologies and offer a more comprehensive description of the research procedures.</p>
            </sec>
        </notes>
    </front>
    <body>
        <sec id="sec5" sec-type="intro">
            <title>Introduction</title>
            <p>Cesarean section is the most common obstetric surgical procedure. The rate of cesarean deliveries increased globally by 19.4 percent in 2018. By 2030, the global cesarean section rate is projected to increase by 30 percent. This is equivalent to 38 million cesarean deliveries according to accumulated data.
                <sup>
                    <xref ref-type="bibr" rid="ref1">1</xref>
                </sup>
            </p>
            <p>In Thailand, the rate of cesarean delivery has shown a continuous upward trend, increasing from 25 percent to 30 percent over the last 15 years, and reaching 30 percent to 50 percent in 2023.
                <sup>
                    <xref ref-type="bibr" rid="ref2">2</xref>
                </sup>
                <sup>&#x2013;</sup>
                <sup>
                    <xref ref-type="bibr" rid="ref4">4</xref>
                </sup> Cesarean sections have been associated with increased anxiety, heightened postpartum traumatic stress symptoms, and reduced well-being during pregnancy compared to vaginal delivery,
                <sup>
                    <xref ref-type="bibr" rid="ref5">5</xref>
                </sup> partically among women who did not initially planned for the procedure.
                <sup>
                    <xref ref-type="bibr" rid="ref6">6</xref>
                </sup>
                <sup>,</sup>
                <sup>
                    <xref ref-type="bibr" rid="ref7">7</xref>
                </sup> Maternal anxiety significantly contributes to postpartum depression. It is a common and serious issue that affects pregnant women, both mentally and emotionally. This can adversely affect one&#x2019;s well-being and family bonding. Furthermore, cesarean delivery significantly increases the risk of postpartum depression.
                <sup>
                    <xref ref-type="bibr" rid="ref8">8</xref>
                </sup> Postpartum depression and anxiety can be influenced by various factors, such as maternal stress, anxiety during pregnancy, stressful events during or after childbirth, traumatic birth experiences, preterm birth, neonatal intensive care, low level of social support, previous history of depression, and breastfeeding problems. Screening for postpartum depression and anxiety during pregnancy using standardized assessment tools is recommended to efficiently identify and support at-risk women.
                <sup>
                    <xref ref-type="bibr" rid="ref9">9</xref>
                </sup>
            </p>
            <p>Previous research has indicated that anesthesia-specific informational videos can reduce anxiety levels and improve patient satisfaction during cesarean section procedures.
                <sup>
                    <xref ref-type="bibr" rid="ref10">10</xref>
                </sup> However, two separate reports have yielded contradictory findings.
                <sup>
                    <xref ref-type="bibr" rid="ref11">11</xref>
                </sup>
                <sup>,</sup>
                <sup>
                    <xref ref-type="bibr" rid="ref12">12</xref>
                </sup>
            </p>
            <p>The inconsistency in these results may be due to the fact that the videos in question focused solely on anesthesia-related information, without encompassing the comprehensive steps of pre-operative and post-operative care or the cesarean section procedure itself.</p>
            <p>A study demonstrated that an informative video covering the entire cesarean process, from pre-operative preparations to post-operative recovery, was effective in reducing maternal anxiety both before and after surgery.
                <sup>
                    <xref ref-type="bibr" rid="ref13">13</xref>
                </sup> This finding suggests that a more holistic and well-structured video presentation may be more effective in alleviating anxiety.</p>
            <p>Nonetheless, no research has been conducted to date in Southeast Asia including Thailand on the use of educational videos to reduce maternal anxiety in mothers preparing for cesarean sections. Such studies would ideally encompass detailed explanations of pre-operative and post-operative care, as well as the procedures that take place within the operating room.</p>
            <p>The aim of this study was to investigate whether providing an informative cesarean section video before surgery would reduce maternal anxiety. Additionally, it aimed to assess the prevalence of anxiety in pregnant women undergoing cesarean delivery and evaluate the satisfaction level with the informative educational video.</p>
        </sec>
        <sec id="sec6" sec-type="methods">
            <title>Methods</title>
            <p>This randomized controlled trial was approved by the Human Ethics Committee of Thammasat University on the 21 March 2023 (MTU-EC-OB-1-010/66) and Thai Clinical Trials Registry on the 28 March 2023 (
                <ext-link ext-link-type="uri" xlink:href="https://www.thaiclinicaltrials.org/show/TCTR20230328001">TCTR20230328001</ext-link>). The study was conducted at the antenatal care clinic and postpartum wards at Thammasat University Hospital, Pathum Thani, Thailand. The participants were Thai singleton pregnant women aged between 18 and 45 years who were scheduled for cesarean delivery between April and September 2023. The exclusion criteria included previous cesarean delivery, pre-existing chronic illnesses before pregnancy, fetal complications during pregnancy, fetal congenital anomalies, severe early neonatal illnesses, pregnancy-related complications that could not be managed by medications, emergency surgery conditions, refusal to participate in this study, and pre-pregnancy mental disorders, such as a history of depression or anxiety, which have been shown to influence the risk of postnatal depression. Therefore, this study aims to minimize confounding factors associated with these conditions.
                <sup>
                    <xref ref-type="bibr" rid="ref7">7</xref>
                </sup>
            </p>
            <p>Patients who met the selection criteria underwent a thorough review before providing written informed consent, as approved by the ethic review committee, to participate in the study. All participants received standard prenatal care and delivery management, regardless of their involvement in the research. On the scheduled day for cesarean delivery, typically 1-2 weeks before the procedure, the research team provided comprehensive explanations about the study, including the randomization process. Participants were then randomly assigned to either the control or intervention group using block randomization. All participants received routine pre- and post-cesarean care, including an explanation of the surgical procedure and potential complications.</p>
            <p>Participants in the intervention group were required to watch a 5-minute informative video, created specifically for this study, on the day of their scheduled cesarean delivery. The video contained no imagery or content that could induce anxiety and provided a detailed explanation of the pre-operative procedures, anesthesia, the cesarean delivery process, and post-operative care.</p>
            <p>Before being implemented in the study, the video underwent a rigorous review by obstetricians at Thammasat University and was tested with patients to ensure clarity and accessibility.</p>
            <p>Before randomization, the study included 178 participants. Sample size calculations, based on pilot data showing 20% post-cesarean anxiety, determined that 77 participants per group were needed to detect a reduction to 5% with the use of an informational video. An additional 10% was added to account for potential data loss, resulting in a total of 170 participants. All participants were required to complete demographic data and State-Trait Anxiety Inventory (STAI form Y-1). The reliability of the STAI was assessed over intervals ranging from one hour to 104 days, with the Trait-Anxiety scale showing reliability coefficients between 0.65 to 0.86.
                <sup>
                    <xref ref-type="bibr" rid="ref14">14</xref>
                </sup> The STAI form Y-1 questionnaire is on state anxiety and consists of 20 items evaluating four levels of intensity: not at all, sometimes, moderately, and very much. The questionnaire comprised 10 positively framed statements (Items 3, 4, 6, 7, 9, 12, 13, 14, 17, and 18), with scores ranging from 1 to 4. It also contained 10 negatively framed statements (Items 1, 2, 5, 8, 10, 11, 15, 16, 19, and 20) with scores ranging from 4 to 1. The total score ranged from 20 to 80 points, with a higher total score indicating a higher level of anxiety. The score interpretation was divided into three ranges of 20-39, 40-59 and 60-80 which can be interpreted as low, moderate, and high levels of anxiety, respectively. On postpartum day 1, all participants were reevaluated using the STAI from Y-1. If a research participant experiences a high level of anxiety before or after cesarean delivery, advice on meeting up with individual psychotherapy will be recommended, or a counseling team will offer assistance to help ease the participant&#x2019;s anxiety. The intervention group was evaluated to measure the level of patient satisfaction with the informative videos on postpartum day 1. Statistical analysis was performed using Statistical Package for the Social Sciences version 26 (SPSS Inc., Chicago, USA). Continuous data were computed using an unpaired t-test and are presented as means with standard deviation. Categorical data were evaluated using the chi-square test and represented as numbers. Statistical significance was set than 0.05.</p>
        </sec>
        <sec id="sec7" sec-type="results">
            <title>Results</title>
            <p>One hundred and seventy-eight participants scheduled for first-time cesarean delivery were recruited and equally randomized into two groups, with eight cases that dropped out, as shown in 
                <xref ref-type="fig" rid="f1">Figure 1</xref>. Maternal demographic and obstetric data between the two groups were statistically indistinguishable, as seen in 
                <xref ref-type="table" rid="T1">Table 1</xref>. Most of the participants were primigravida, had an educational level of at least a bachelor&#x2019;s degree, and did not have any underlying disease or previous abdominal surgery. No differences were found in the indications for cesarean section and incidence of postpartum hemorrhage in either group. There were no cases of serious post-operative complications such as re-exploration or neonatal intensive care unit admission. The information sources of cesarean delivery in both groups were similar, and most of the participants received information about cesarean delivery from the Internet and friends before joining the research, as shown in 
                <xref ref-type="fig" rid="f2">Figure 2</xref>.</p>
            <fig fig-type="figure" id="f1" orientation="portrait" position="float">
                <label>Figure 1. </label>
                <caption>
                    <title>Flow chart of study.</title>
                    <p>Control: standard pre-cesarean section operative care; Video: informative cesarean section operative steps video before cesarean section operation; STAI: State-Trait Anxiety Inventory.</p>
                    <p>QR code for informative cesarean section operative steps video.</p>
                </caption>
                <graphic id="gr1" orientation="portrait" position="float" xlink:href="https://f1000research-files.f1000.com/manuscripts/171685/51c02729-eca3-4f36-af86-db5ad32fe19d_figure1.gif"/>
            </fig>
            <table-wrap id="T1" orientation="portrait" position="float">
                <label>Table 1. </label>
                <caption>
                    <title>Demographic and obstetric characters of pregnant women who underwent first time cesarean section (n=85 cases per group).</title>
                </caption>
                <table content-type="article-table" frame="hsides">
                    <thead>
                        <tr>
                            <th align="left" colspan="1" rowspan="1" valign="top"/>
                            <th align="left" colspan="1" rowspan="1" valign="top">Control</th>
                            <th align="left" colspan="1" rowspan="1" valign="top">Video</th>
                            <th align="left" colspan="1" rowspan="1" valign="top">p-value</th>
                        </tr>
                    </thead>
                    <tbody>
                        <tr>
                            <td align="left" colspan="1" rowspan="1" valign="top">Age (years)
                                <xref ref-type="table-fn" rid="tfn1">*</xref>
                            </td>
                            <td align="left" colspan="1" rowspan="1" valign="top">32.07 &#x00b1; 4.8</td>
                            <td align="left" colspan="1" rowspan="1" valign="top">30.79 &#x00b1; 5.3</td>
                            <td align="left" colspan="1" rowspan="1" valign="top">0.100</td>
                        </tr>
                        <tr>
                            <td align="left" colspan="1" rowspan="1" valign="top">BMI (kg/m
                                <sup>2</sup>)
                                <xref ref-type="table-fn" rid="tfn1">*</xref>
                            </td>
                            <td align="left" colspan="1" rowspan="1" valign="top">22.80 &#x00b1; 4.5</td>
                            <td align="left" colspan="1" rowspan="1" valign="top">23.56 &#x00b1; 5.2</td>
                            <td align="left" colspan="1" rowspan="1" valign="top">0.305</td>
                        </tr>
                        <tr>
                            <td align="left" colspan="1" rowspan="1" valign="top">Nulliparity
                                <xref ref-type="table-fn" rid="tfn2">**</xref>
                            </td>
                            <td align="left" colspan="1" rowspan="1" valign="top">60 (70.6)</td>
                            <td align="left" colspan="1" rowspan="1" valign="top">63 (74.1)</td>
                            <td align="left" colspan="1" rowspan="1" valign="top">0.607</td>
                        </tr>
                        <tr>
                            <td align="left" colspan="1" rowspan="1" valign="top">Education level
                                <xref ref-type="table-fn" rid="tfn2">**</xref>
                            </td>
                            <td colspan="1" rowspan="1"/>
                            <td colspan="1" rowspan="1"/>
                            <td align="left" colspan="1" rowspan="1" valign="top">0.123</td>
                        </tr>
                        <tr>
                            <td align="left" colspan="1" rowspan="1" valign="top">&#x2003;
                                <inline-formula>
                                    <mml:math display="inline">
                                        <mml:mo>&#x2264;</mml:mo>
                                    </mml:math>
                                </inline-formula> Secondary</td>
                            <td align="left" colspan="1" rowspan="1" valign="top">19 (22.4)</td>
                            <td align="left" colspan="1" rowspan="1" valign="top">28 (32.9)</td>
                            <td colspan="1" rowspan="1"/>
                        </tr>
                        <tr>
                            <td align="left" colspan="1" rowspan="1" valign="top">&#x2003;
                                <inline-formula>
                                    <mml:math display="inline">
                                        <mml:mo>&#x2265;</mml:mo>
                                    </mml:math>
                                </inline-formula> Bachelor</td>
                            <td align="left" colspan="1" rowspan="1" valign="top">66 (77.6)</td>
                            <td align="left" colspan="1" rowspan="1" valign="top">57 (67.1)</td>
                            <td colspan="1" rowspan="1"/>
                        </tr>
                        <tr>
                            <td align="left" colspan="1" rowspan="1" valign="top">No underlying disease
                                <xref ref-type="table-fn" rid="tfn2">**</xref>
                            </td>
                            <td align="left" colspan="1" rowspan="1" valign="top">75 (88.2)</td>
                            <td align="left" colspan="1" rowspan="1" valign="top">79 (92.9)</td>
                            <td align="left" colspan="1" rowspan="1" valign="top">0.607</td>
                        </tr>
                        <tr>
                            <td align="left" colspan="1" rowspan="1" valign="top">No history of abortion
                                <xref ref-type="table-fn" rid="tfn2">**</xref>
                            </td>
                            <td align="left" colspan="1" rowspan="1" valign="top">67 (78.8)</td>
                            <td align="left" colspan="1" rowspan="1" valign="top">74 (87.1)</td>
                            <td align="left" colspan="1" rowspan="1" valign="top">0.153</td>
                        </tr>
                        <tr>
                            <td align="left" colspan="1" rowspan="1" valign="top">No history of abdominal surgery
                                <xref ref-type="table-fn" rid="tfn2">**</xref>
                            </td>
                            <td align="left" colspan="1" rowspan="1" valign="top">75 (88.2)</td>
                            <td align="left" colspan="1" rowspan="1" valign="top">76 (89.4)</td>
                            <td align="left" colspan="1" rowspan="1" valign="top">0.808</td>
                        </tr>
                        <tr>
                            <td align="left" colspan="1" rowspan="1" valign="top">Indication
                                <xref ref-type="table-fn" rid="tfn2">**</xref>
                            </td>
                            <td colspan="1" rowspan="1"/>
                            <td colspan="1" rowspan="1"/>
                            <td align="left" colspan="1" rowspan="1" valign="top">0.583</td>
                        </tr>
                        <tr>
                            <td align="left" colspan="1" rowspan="1" valign="top">&#x2003;Maternal request</td>
                            <td align="left" colspan="1" rowspan="1" valign="top">52 (61.2)</td>
                            <td align="left" colspan="1" rowspan="1" valign="top">60 (70.6)</td>
                            <td colspan="1" rowspan="1"/>
                        </tr>
                        <tr>
                            <td align="left" colspan="1" rowspan="1" valign="top">&#x2003;Malpresentation</td>
                            <td align="left" colspan="1" rowspan="1" valign="top">14 (16.5)</td>
                            <td align="left" colspan="1" rowspan="1" valign="top">14 (16.5)</td>
                            <td colspan="1" rowspan="1"/>
                        </tr>
                        <tr>
                            <td align="left" colspan="1" rowspan="1" valign="top">&#x2003;Obstructed labor</td>
                            <td align="left" colspan="1" rowspan="1" valign="top">11 (12.9)</td>
                            <td align="left" colspan="1" rowspan="1" valign="top">6 (7.1)</td>
                            <td colspan="1" rowspan="1"/>
                        </tr>
                        <tr>
                            <td align="left" colspan="1" rowspan="1" valign="top">&#x2003;Fetal macrosomia</td>
                            <td align="left" colspan="1" rowspan="1" valign="top">4 (4.7)</td>
                            <td align="left" colspan="1" rowspan="1" valign="top">2 (2.4)</td>
                            <td colspan="1" rowspan="1"/>
                        </tr>
                        <tr>
                            <td align="left" colspan="1" rowspan="1" valign="top">&#x2003;Placenta previa</td>
                            <td align="left" colspan="1" rowspan="1" valign="top">4 (4.7)</td>
                            <td align="left" colspan="1" rowspan="1" valign="top">3 (3.5)</td>
                            <td colspan="1" rowspan="1"/>
                        </tr>
                        <tr>
                            <td align="left" colspan="1" rowspan="1" valign="top">TR
                                <xref ref-type="table-fn" rid="tfn2">**</xref>
                            </td>
                            <td align="left" colspan="1" rowspan="1" valign="top">5 (5.9)</td>
                            <td align="left" colspan="1" rowspan="1" valign="top">7 (8.2)</td>
                            <td align="left" colspan="1" rowspan="1" valign="top">0.549</td>
                        </tr>
                        <tr>
                            <td align="left" colspan="1" rowspan="1" valign="top">Weight of newborn
                                <xref ref-type="table-fn" rid="tfn1">*</xref>
                            </td>
                            <td align="left" colspan="1" rowspan="1" valign="top">3172.0 &#x00b1; 406.6</td>
                            <td align="left" colspan="1" rowspan="1" valign="top">3098.0 &#x00b1; 478.0</td>
                            <td align="left" colspan="1" rowspan="1" valign="top">0.278</td>
                        </tr>
                        <tr>
                            <td align="left" colspan="1" rowspan="1" valign="top">Postpartum hemorrhage
                                <xref ref-type="table-fn" rid="tfn2">**</xref>
                            </td>
                            <td align="left" colspan="1" rowspan="1" valign="top">4 (4.7)</td>
                            <td align="left" colspan="1" rowspan="1" valign="top">2 (2.4)</td>
                            <td align="left" colspan="1" rowspan="1" valign="top">0.406</td>
                        </tr>
                    </tbody>
                </table>
                <table-wrap-foot>
                    <fn-group content-type="footnotes">
                        <fn id="tfn1">
                            <label>
                                <sup>*</sup>
                            </label>
                            <p>: mean &#x00b1; standard deviation (SD).</p>
                        </fn>
                        <fn id="tfn2">
                            <label>
                                <sup>**</sup>
                            </label>
                            <p>: n (%); Control: standard pre-cesarean operation care; Video: informative cesarean section steps video before operation; BMI: body mass index; TR: Tubal sterilization operation.</p>
                        </fn>
                    </fn-group>
                </table-wrap-foot>
            </table-wrap>
            <fig fig-type="figure" id="f2" orientation="portrait" position="float">
                <label>Figure 2. </label>
                <caption>
                    <title>Information source of cesarean delivery before operation (not include intervention) (n=85 cases per group).</title>
                    <p>Control: standard pre-cesarean operation care; Video: informative cesarean section steps video before operation; Book: The book that provides information on preparing for cesarean delivery; Family: A group of people who are related by blood, marriage or adoption such as parents; Medical professionals: person individuals trained and qualified to provide medical care such as doctors, nurses and other healthcare providers; Friends: person whom one has a bound of mutual affection who not a member of family; Internet: internet information providing guidance on preparing for cesarean delivery.</p>
                </caption>
                <graphic id="gr2" orientation="portrait" position="float" xlink:href="https://f1000research-files.f1000.com/manuscripts/171685/51c02729-eca3-4f36-af86-db5ad32fe19d_figure2.gif"/>
            </fig>
            <p>Before cesarean delivery, the STAI scores of the intervention and control groups did not differ significantly. The prevalence of participants who had moderate to high levels of anxiety in this study was 71 percent, eight of whom had high STAI scores.</p>
            <p>Post-operatively, the STAI score was statistically significant in both groups. However, upon juxtaposing the post-operative scores between the two groups, it became evident that the cohort exposed to the intervention group had a statistically noteworthy decrease in scores compared to the control group (decreasing mean STAI score were 7.0 and 4.4 in the intervention and control groups, respectively, p=0.002), as shown in 
                <xref ref-type="fig" rid="f3">Figure 3</xref>. 
                <xref ref-type="table" rid="T2">Table 2</xref> shows that the control group exhibited a pre- operative moderate-to-high anxiety level of 77 percent, which was reduced to 53 percent post-operatively. The intervention group showed that the proportion of patients with moderate-to-high anxiety levels decreased from 65 percent in the pre-operative period to 18% in the post-operative period.</p>
            <fig fig-type="figure" id="f3" orientation="portrait" position="float">
                <label>Figure 3. </label>
                <caption>
                    <title>Comparison STAI score of pregnant women who underwent first time cesarean section (n=85 cases per group).</title>
                    <p>STAI: The State-Trait Anxiety Inventory; Control: standard pre-cesarean operation care; Video: informative cesarean section steps video before operation; Before: anxiety score before cesarean section; After: anxiety score after cesarean section.</p>
                </caption>
                <graphic id="gr3" orientation="portrait" position="float" xlink:href="https://f1000research-files.f1000.com/manuscripts/171685/51c02729-eca3-4f36-af86-db5ad32fe19d_figure3.gif"/>
            </fig>
            <table-wrap id="T2" orientation="portrait" position="float">
                <label>Table 2. </label>
                <caption>
                    <title>Comparison STAI score of pregnant women who underwent first time cesarean section (n=85 cases per group).</title>
                </caption>
                <table content-type="article-table" frame="hsides">
                    <thead>
                        <tr>
                            <th align="left" colspan="1" rowspan="2" valign="top">STAI levels</th>
                            <th align="left" colspan="3" rowspan="1" valign="top">Control</th>
                            <th align="left" colspan="3" rowspan="1" valign="top">Video</th>
                        </tr>
                        <tr>
                            <th align="left" colspan="1" rowspan="1" valign="top">Before</th>
                            <th align="left" colspan="1" rowspan="1" valign="top">After</th>
                            <th align="left" colspan="1" rowspan="1" valign="top">p-value</th>
                            <th align="left" colspan="1" rowspan="1" valign="top">Before</th>
                            <th align="left" colspan="1" rowspan="1" valign="top">After</th>
                            <th align="left" colspan="1" rowspan="1" valign="top">p-value</th>
                        </tr>
                    </thead>
                    <tbody>
                        <tr>
                            <td align="left" colspan="1" rowspan="1" valign="top">&#x2003;Low</td>
                            <td align="left" colspan="1" rowspan="1" valign="top">19</td>
                            <td align="left" colspan="1" rowspan="1" valign="top">40</td>
                            <td align="left" colspan="1" rowspan="1" valign="top">&lt;0.001</td>
                            <td align="left" colspan="1" rowspan="1" valign="top">30</td>
                            <td align="left" colspan="1" rowspan="1" valign="top">70</td>
                            <td align="left" colspan="1" rowspan="1" valign="top">&lt;0.001</td>
                        </tr>
                        <tr>
                            <td align="left" colspan="1" rowspan="1" valign="top">&#x2003;Moderate&#x2013;High</td>
                            <td align="left" colspan="1" rowspan="1" valign="top">66</td>
                            <td align="left" colspan="1" rowspan="1" valign="top">45</td>
                            <td colspan="1" rowspan="1"/>
                            <td align="left" colspan="1" rowspan="1" valign="top">55</td>
                            <td align="left" colspan="1" rowspan="1" valign="top">15</td>
                            <td colspan="1" rowspan="1"/>
                        </tr>
                    </tbody>
                </table>
                <table-wrap-foot>
                    <p>STAI: State-Trait Anxiety Inventory (range 20-80); Control: standard pre-cesarean operation care; Video: informative cesarean section steps video before operation; before: anxiety score before cesarean section; after: anxiety score after cesarean section; Low: low level of anxiety (score 20-39); Moderate: moderate level of anxiety (score 40-59); High: high level of anxiety (score 60-80).</p>
                </table-wrap-foot>
            </table-wrap>
            <p>Seventy-eight percent of intervention patients transitioned from moderate to high anxiety pre-operatively to low anxiety post-operatively, doubling the control group's 34 percent improvement. Furthermore, two patients in the control group showed escalation from moderate to high anxiety levels, whereas in the intervention group, patients with high anxiety levels pre-operatively had reduced anxiety levels. Notably, all patients with pre- and post-operative high anxiety received psychological counseling. In the intervention group, almost all individuals reported the highest satisfaction scores for the informative cesarean section operative steps video, reaching approximately 87 percent.</p>
            <p>According to the evaluation of factors affecting pre- and post-operative anxiety scores, pre-operative cesarean section anxiety was not affected. While two factors, watching the informative operative steps video and pre-operative anxiety scores, had a statistically significant impact on the post-operative anxiety score, the odds ratios were 0.17 and 8.23, respectively. as shown in 
                <xref ref-type="table" rid="T3">Table 3</xref>. Watching the informative video had potentially reduced stress by 83%, and experiencing anxiety before surgery increased the risk of post-operative anxiety stress by 8.23 times.</p>
            <table-wrap id="T3" orientation="portrait" position="float">
                <label>Table 3. </label>
                <caption>
                    <title>Logistic regression analysis to determine independents associated factors for pre- and post-operative STAI score.</title>
                </caption>
                <table content-type="article-table" frame="hsides">
                    <thead>
                        <tr>
                            <th align="left" colspan="1" rowspan="1" valign="top"/>
                            <th align="left" colspan="1" rowspan="1" valign="top">Adjusted OR</th>
                            <th align="left" colspan="1" rowspan="1" valign="top">95 % CI</th>
                            <th align="left" colspan="1" rowspan="1" valign="top">p-value</th>
                        </tr>
                    </thead>
                    <tbody>
                        <tr>
                            <td align="left" colspan="1" rowspan="1" valign="top">Pre-operative STAI score</td>
                            <td colspan="1" rowspan="1"/>
                            <td colspan="1" rowspan="1"/>
                            <td colspan="1" rowspan="1"/>
                        </tr>
                        <tr>
                            <td align="left" colspan="1" rowspan="1" valign="top">&#x2003;AMA</td>
                            <td align="left" colspan="1" rowspan="1" valign="top">0.78</td>
                            <td align="left" colspan="1" rowspan="1" valign="top">0.36&#x2013;1.72</td>
                            <td align="left" colspan="1" rowspan="1" valign="top">0.537</td>
                        </tr>
                        <tr>
                            <td align="left" colspan="1" rowspan="1" valign="top">&#x2003;Nulliparity</td>
                            <td align="left" colspan="1" rowspan="1" valign="top">1.34</td>
                            <td align="left" colspan="1" rowspan="1" valign="top">0.44&#x2013;4.10</td>
                            <td align="left" colspan="1" rowspan="1" valign="top">0.614</td>
                        </tr>
                        <tr>
                            <td align="left" colspan="1" rowspan="1" valign="top">&#x2003;Education level 
                                <inline-formula>
                                    <mml:math display="inline">
                                        <mml:mo>&#x2264;</mml:mo>
                                    </mml:math>
                                </inline-formula> Secondary</td>
                            <td align="left" colspan="1" rowspan="1" valign="top">1.62</td>
                            <td align="left" colspan="1" rowspan="1" valign="top">0.67&#x2013;3.94</td>
                            <td align="left" colspan="1" rowspan="1" valign="top">0.289</td>
                        </tr>
                        <tr>
                            <td align="left" colspan="1" rowspan="1" valign="top">&#x2003;Indication: maternal request</td>
                            <td align="left" colspan="1" rowspan="1" valign="top">0.82</td>
                            <td align="left" colspan="1" rowspan="1" valign="top">0.38&#x2013;1.76</td>
                            <td align="left" colspan="1" rowspan="1" valign="top">0.603</td>
                        </tr>
                        <tr>
                            <td align="left" colspan="1" rowspan="1" valign="top">Post-operative STAI score</td>
                            <td colspan="1" rowspan="1"/>
                            <td colspan="1" rowspan="1"/>
                            <td colspan="1" rowspan="1"/>
                        </tr>
                        <tr>
                            <td align="left" colspan="1" rowspan="1" valign="top">&#x2003;AMA</td>
                            <td align="left" colspan="1" rowspan="1" valign="top">0.60</td>
                            <td align="left" colspan="1" rowspan="1" valign="top">0.24&#x2013;1.49</td>
                            <td align="left" colspan="1" rowspan="1" valign="top">0.269</td>
                        </tr>
                        <tr>
                            <td align="left" colspan="1" rowspan="1" valign="top">&#x2003;Nulliparity</td>
                            <td align="left" colspan="1" rowspan="1" valign="top">0.79</td>
                            <td align="left" colspan="1" rowspan="1" valign="top">0.24&#x2013;2.63</td>
                            <td align="left" colspan="1" rowspan="1" valign="top">0.706</td>
                        </tr>
                        <tr>
                            <td align="left" colspan="1" rowspan="1" valign="top">&#x2003;Education level 
                                <inline-formula>
                                    <mml:math display="inline">
                                        <mml:mo>&#x2264;</mml:mo>
                                    </mml:math>
                                </inline-formula> Secondary</td>
                            <td align="left" colspan="1" rowspan="1" valign="top">1.72</td>
                            <td align="left" colspan="1" rowspan="1" valign="top">0.67&#x2013;4.47</td>
                            <td align="left" colspan="1" rowspan="1" valign="top">0.263</td>
                        </tr>
                        <tr>
                            <td align="left" colspan="1" rowspan="1" valign="top">&#x2003;Indication: maternal request</td>
                            <td align="left" colspan="1" rowspan="1" valign="top">1.85</td>
                            <td align="left" colspan="1" rowspan="1" valign="top">0.79&#x2013;4.34</td>
                            <td align="left" colspan="1" rowspan="1" valign="top">0.157</td>
                        </tr>
                        <tr>
                            <td align="left" colspan="1" rowspan="1" valign="top">&#x2003;Postpartum hemorrhage</td>
                            <td align="left" colspan="1" rowspan="1" valign="top">4.01</td>
                            <td align="left" colspan="1" rowspan="1" valign="top">0.49&#x2013;32.91</td>
                            <td align="left" colspan="1" rowspan="1" valign="top">0.196</td>
                        </tr>
                        <tr>
                            <td align="left" colspan="1" rowspan="1" valign="top">&#x2003;Video</td>
                            <td align="left" colspan="1" rowspan="1" valign="top">0.17</td>
                            <td align="left" colspan="1" rowspan="1" valign="top">0.07&#x2013;0.38</td>
                            <td align="left" colspan="1" rowspan="1" valign="top">&lt;0.001</td>
                        </tr>
                        <tr>
                            <td align="left" colspan="1" rowspan="1" valign="top">&#x2003;Pre-operative anxiety scores</td>
                            <td align="left" colspan="1" rowspan="1" valign="top">8.23</td>
                            <td align="left" colspan="1" rowspan="1" valign="top">2.70&#x2013;25.02</td>
                            <td align="left" colspan="1" rowspan="1" valign="top">&lt;0.001</td>
                        </tr>
                    </tbody>
                </table>
                <table-wrap-foot>
                    <p>STAI: State-Trait Anxiety Inventory; AMA: Advance maternal age 
                        <inline-formula>
                            <mml:math display="inline">
                                <mml:mo>&#x2265;</mml:mo>
                            </mml:math>
                        </inline-formula> 35 years; Video: Watching the informative operation steps video.</p>
                </table-wrap-foot>
            </table-wrap>
        </sec>
        <sec id="sec8" sec-type="discussion">
            <title>Discussion</title>
            <p>Maternal anxiety is prevalent throughout pregnancy, childbirth, and postpartum, with reported rates ranging from 15 percent to 36 percent.
                <sup>
                    <xref ref-type="bibr" rid="ref15">15</xref>
                </sup>
                <sup>&#x2013;</sup>
                <sup>
                    <xref ref-type="bibr" rid="ref18">18</xref>
                </sup> Some studies suggest an increase in self-reported anxiety symptoms with advancing gestational age, peaking in the third trimester, and even exceeding postpartum levels.
                <sup>
                    <xref ref-type="bibr" rid="ref18">18</xref>
                </sup> Interestingly, our study found that in planned cesarean section, patients exhibited significantly higher anxiety levels (71 percent) compared to their counterparts without planned birth interventions during the third trimester.
                <sup>
                    <xref ref-type="bibr" rid="ref18">18</xref>
                </sup> This suggests a potential correlation between cesarean anticipation and heightened anxiety.</p>
            <p>Cesarean section is the most common procedure in obstetrics and has been proven to save numerous lives of both mothers and infants during pregnancy complications. In some cases, parents may request this delivery method. There are various ways to access information regarding cesarean deliveries. However, despite its prevalence and readily available information, patients undergoing planned cesarean delivery still exhibit higher anxiety levels. Therefore, the ACOG strongly recommends assessing anxiety risks and conditions in pregnant and postpartum women for early detection and intervention.
                <sup>
                    <xref ref-type="bibr" rid="ref9">9</xref>
                </sup> Interventions for patients with anxiety can include both pharmacological and non-pharmacological methods, such as individual or group psychotherapy, music therapy, massage, meditation and hypnotherapy.
                <sup>
                    <xref ref-type="bibr" rid="ref19">19</xref>
                </sup>
                <sup>&#x2013;</sup>
                <sup>
                    <xref ref-type="bibr" rid="ref21">21</xref>
                </sup>
            </p>
            <p>Current recommendations suggest implementing interventions to reduce anxiety before or near the time of cesarean section.
                <sup>
                    <xref ref-type="bibr" rid="ref22">22</xref>
                </sup> While some studies using informative videos about spinal block have not shown significant anxiety reduction,
                <sup>
                    <xref ref-type="bibr" rid="ref10">10</xref>
                </sup>
                <sup>&#x2013;</sup>
                <sup>
                    <xref ref-type="bibr" rid="ref12">12</xref>
                </sup>
                <sup>,</sup>
                <sup>
                    <xref ref-type="bibr" rid="ref23">23</xref>
                </sup>
                <sup>,</sup>
                <sup>
                    <xref ref-type="bibr" rid="ref24">24</xref>
                </sup> research utilizing videos specifically about the cesarean operation process itself demonstrated a noteworthy decrease.
                <sup>
                    <xref ref-type="bibr" rid="ref13">13</xref>
                </sup> This aligns with our study's finding of reduced post-operative anxiety levels. Interestingly, both the intervention and control groups showed lower anxiety levels post-operatively. This may be attributed to the successful completion of surgery and the absence of complications, leading to a reduction in natural anxiety in both groups. However, a noteworthy detail emerged: the intervention group displayed a higher reduction in the STAI score post-operatively compared to the control group. Furthermore, the results revealed a shift within the intervention group, with 78 percent of patients initially experiencing moderate-to-high anxiety transitioning to a low anxiety level post-operatively, compared to only 34 percent in the control group. This substantial improvement reflects a greater efficacy of the intervention in mitigating presurgical anxiety compared to the control group. Notably, the overall prevalence of moderate anxiety in the intervention group post-operatively was 15 percent, which is consistent with the study of cesarean operative anxiety levels observed in Cindy-Lee's study involving unplanned cesarean operations and natural births. In stark contrast, the control group exhibited a significantly higher prevalence of moderate-to-high anxiety post-operatively, reaching 68 percent.
                <sup>
                    <xref ref-type="bibr" rid="ref18">18</xref>
                </sup> All pre-operative intervention patients who received psychological counseling showed no remaining cases of high anxiety level post-surgery, suggesting the effectiveness of counseling in reducing anxiety. However, both groups saw 10 percent (five cases) in the low anxiety group to moderate anxiety. Unfortunately, due to the limited sample size, a definitive analysis of the causes of this shift was not performed.</p>
            <p>Previous research has identified several factors that significantly influence antenatal anxiety levels, including low to middle income, low educational attainment, a history of abortions, and the responsibility of caring for other children. Additionally, postnatal concerns, such as breastfeeding difficulties, wound pain, and infant care have been linked to post-operative anxiety.
                <sup>
                    <xref ref-type="bibr" rid="ref9">9</xref>
                </sup>
                <sup>,</sup>
                <sup>
                    <xref ref-type="bibr" rid="ref16">16</xref>
                </sup> Interestingly, our study found that none of the previously identified factors, even a history of abortions, significantly impacted pre-operative anxiety. Instead, watching informative videos before surgery demonstrably reduced anxiety levels post-operatively, consistent with findings from many studies.
                <sup>
                    <xref ref-type="bibr" rid="ref10">10</xref>
                </sup>
                <sup>,</sup>
                <sup>
                    <xref ref-type="bibr" rid="ref13">13</xref>
                </sup>
                <sup>,</sup>
                <sup>
                    <xref ref-type="bibr" rid="ref25">25</xref>
                </sup>
            </p>
            <p>However, a limitation of our study is that it solely assessed anxiety on postpartum day 1 by using the STAI.
                <sup>
                    <xref ref-type="bibr" rid="ref14">14</xref>
                </sup> Consequently, factors such as breastfeeding difficulties, wound pain, and infant care challenges, which often arise later in the postnatal period, were not addressed. Previous studies have indicated that postnatal depression can persist for up to 2-3 years.
                <sup>
                    <xref ref-type="bibr" rid="ref6">6</xref>
                </sup>
                <sup>,</sup>
                <sup>
                    <xref ref-type="bibr" rid="ref7">7</xref>
                </sup> Therefore, further research to develop more effective intervention methods and tools aimed at reducing anxiety during pregnancy and motherhood should be considered.</p>
            <p>The findings from this research show that pre-operative video education has emerged as a powerful tool for reducing cesarean operative anxiety, offering promise as a safe, cost-effective, and widely accessible intervention. These findings suggest that informative videos can be disseminated beyond hospital walls, offering benefits to patients who might not otherwise have access to accurate and reliable information. The ability to disseminate and empower patients with clear and accurate pre-operative information regardless of their source of prior knowledge is crucial. In particular, when considering the varied sources from which patients could acquire information pre-operatively, including personal experiences, hearsay, and academic resources. This study highlights the need for comprehensive and reliable pre-operative information to ensure that patients feel empowered throughout their surgical journey.</p>
            <p>Furthermore, leveraging technology and digital platforms could expand the reach of educational resources, ensuring that accurate and comprehensive information is easily accessible to a wider audience, creating easily digestible and reliable content that can empower pregnant patients and enable them to make informed decisions and better prepare for surgical procedures. This patient-centered approach not only enhances care and satisfaction but also contributes to improved health outcomes.</p>
        </sec>
        <sec id="sec9" sec-type="conclusion">
            <title>Conclusion</title>
            <p>Pre-operative educational videos have emerged as a powerful tool that significantly reduces cesarean operative anxiety and holds promise for improved health outcomes. This readily implementable intervention empowers women with clear and reliable information, transcends hospital walls, and promotes informed decision making. Further research should explore its long-term impact and applicability to other surgical contexts to implement positive surgical experiences. Investing in this approach will not only equip mothers with confidence but also lay the groundwork for a healthier future for mothers and their families.</p>
        </sec>
        <sec id="sec11">
            <title>Ethics and consent</title>
            <p>This randomized controlled trial was approved by the Human Ethics Committee of Thammasat University on the 21 March 2023 (MTU-EC-OB-1-010/66) and Thai Clinical Trials Registry on the 28 March 2023 (TCTR20230328001). Patients who met the selection criteria were reviewed before they were asked to provide written informed consent approved by the ethical approval committee to participate in the project.</p>
        </sec>
    </body>
    <back>
        <sec id="sec14" sec-type="data availability">
            <title>Data availability</title>
            <sec id="sec15">
                <title>Underlying data</title>
                <p>Zenodo: Effect of informative cesarean delivery operative steps video to maternal anxiety level: a randomized controlled trial, 
                    <ext-link ext-link-type="uri" xlink:href="https://zenodo.org/records/11372557">https://zenodo.org/records/11372557</ext-link>.
                    <sup>

                        <xref ref-type="bibr" rid="ref26">26</xref>
</sup>
                </p>
                <p>This project contains following datasets:
                    <list list-type="order">
                        <list-item>
                            <label>1.</label>
                            <p>

                                <ext-link ext-link-type="uri" xlink:href="https://zenodo.org/records/11372557/files/Effect%20of%20Informative%20Cesarean%20Delivery%20Operative%20Steps%20Video%20to%20Maternal%20Anxiety%20Level.xlsx?download=1">Effect of Informative Cesarean Delivery Operative Steps Video to Maternal Anxiety Level.xlsx</ext-link>
                            </p>
                        </list-item>
                        <list-item>
                            <label>2.</label>
                            <p>

                                <ext-link ext-link-type="uri" xlink:href="https://zenodo.org/records/11372557/files/information%20cesarean%20section%20(english%20sub).mp4?download=1">information cesarean section (english sub).mp4</ext-link>
                            </p>
                        </list-item>
                        <list-item>
                            <label>3.</label>
                            <p>

                                <ext-link ext-link-type="uri" xlink:href="https://zenodo.org/records/11372557/files/Questionnaire.docx?download=1">Questionnaire.docx</ext-link>
                            </p>
                        </list-item>
                    </list>
                </p>
                <p>Data are available under the terms of the 
                    <ext-link ext-link-type="uri" xlink:href="https://creativecommons.org/licenses/by/4.0/">Creative Commons Attribution 4.0 International license</ext-link> (CC-BY 4.0).</p>
            </sec>
            <sec id="sec16">
                <title>Extended data</title>
                <p>Zenodo: Effect of informative cesarean delivery operative steps video to maternal anxiety level: a randomized controlled trial, 
                    <ext-link ext-link-type="uri" xlink:href="https://zenodo.org/records/11372557">https://zenodo.org/records/11372557</ext-link>.
                    <sup>

                        <xref ref-type="bibr" rid="ref26">26</xref>
</sup>
                </p>
                <p>This project contains following datasets:
                    <list list-type="order">
                        <list-item>
                            <label>1.</label>
                            <p>

                                <ext-link ext-link-type="uri" xlink:href="https://zenodo.org/records/11372557/files/CONSORT-2010-Checklist.doc?download=1">CONSORT-2010-Checklist.doc</ext-link>
                            </p>
                        </list-item>
                        <list-item>
                            <label>2.</label>
                            <p>

                                <ext-link ext-link-type="uri" xlink:href="https://zenodo.org/records/11372557/files/Consent%20Form.docx?download=1">Consent Form.docx</ext-link>
                            </p>
                        </list-item>
                    </list>
                </p>
                <p>Data are available under the terms of the 
                    <ext-link ext-link-type="uri" xlink:href="https://creativecommons.org/licenses/by/4.0/">Creative Commons Attribution 4.0 International license</ext-link> (CC-BY 4.0).</p>
            </sec>
        </sec>
        <sec id="sec10">
            <title>Reporting guidelines</title>
            <p>Zenodo: CONSORT checklist for &#x2018;Effect of informative cesarean delivery operative steps video to maternal anxiety level: a randomized controlled trial&#x2019;, 
                <ext-link ext-link-type="uri" xlink:href="https://zenodo.org/records/11372557">https://zenodo.org/records/11372557</ext-link>.</p>
            <p>Data are available under the terms of the 
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        </sec>
        <ack>
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            <p>The authors thank the Clin. Prof. Dittakarn Boriboonhirunsarn kindly helped with the statistical analysis.</p>
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                        <italic toggle="yes">BMC. Psychol.</italic>
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    <sub-article article-type="reviewer-report" id="report324077">
        <front-stub>
            <article-id pub-id-type="doi">10.5256/f1000research.171685.r324077</article-id>
            <title-group>
                <article-title>Reviewer response for version 2</article-title>
            </title-group>
            <contrib-group>
                <contrib contrib-type="author">
                    <name>
                        <surname>La Rosa</surname>
                        <given-names>Valentina Lucia</given-names>
                    </name>
                    <xref ref-type="aff" rid="r324077a1">1</xref>
                    <role>Referee</role>
                    <uri content-type="orcid">https://orcid.org/0000-0002-6619-6777</uri>
                </contrib>
                <aff id="r324077a1">
                    <label>1</label>Department of Educational Sciences, University of Catania, Catania, Italy</aff>
            </contrib-group>
            <author-notes>
                <fn fn-type="conflict">
                    <p>
                        <bold>Competing interests: </bold>No competing interests were disclosed.</p>
                </fn>
            </author-notes>
            <pub-date pub-type="epub">
                <day>24</day>
                <month>9</month>
                <year>2024</year>
            </pub-date>
            <permissions>
                <copyright-statement>Copyright: &#x00a9; 2024 La Rosa VL</copyright-statement>
                <copyright-year>2024</copyright-year>
                <license xlink:href="https://creativecommons.org/licenses/by/4.0/">
                    <license-p>This is an open access peer review report distributed under the terms of the Creative Commons Attribution Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.</license-p>
                </license>
            </permissions>
            <related-article ext-link-type="doi" id="relatedArticleReport324077" related-article-type="peer-reviewed-article" xlink:href="10.12688/f1000research.147840.2"/>
            <custom-meta-group>
                <custom-meta>
                    <meta-name>recommendation</meta-name>
                    <meta-value>approve</meta-value>
                </custom-meta>
            </custom-meta-group>
        </front-stub>
        <body>
            <p>I have reviewed the revision of the manuscript entitled "Effect of Informative Cesarean Delivery Operative Steps Video on Maternal Anxiety Level: A Randomized Controlled Trial". The authors have satisfactorily addressed all critical issues and concerns raised in the first revision. The revisions have significantly improved the clarity and quality of the manuscript. The methodology is now well articulated, and the results are more fully presented.</p>
            <p> </p>
            <p> I believe the manuscript now meets the journal's standards and makes a valuable contribution to the field. I therefore recommend that it be accepted for indexing.</p>
            <p>Is the work clearly and accurately presented and does it cite the current literature?</p>
            <p>Partly</p>
            <p>If applicable, is the statistical analysis and its interpretation appropriate?</p>
            <p>Yes</p>
            <p>Are all the source data underlying the results available to ensure full reproducibility?</p>
            <p>Partly</p>
            <p>Is the study design appropriate and is the work technically sound?</p>
            <p>Yes</p>
            <p>Are the conclusions drawn adequately supported by the results?</p>
            <p>Partly</p>
            <p>Are sufficient details of methods and analysis provided to allow replication by others?</p>
            <p>Partly</p>
            <p>Reviewer Expertise:</p>
            <p>Developmental and perinatal psychology</p>
            <p>I confirm that I have read this submission and believe that I have an appropriate level of expertise to confirm that it is of an acceptable scientific standard.</p>
        </body>
    </sub-article>
    <sub-article article-type="reviewer-report" id="report297337">
        <front-stub>
            <article-id pub-id-type="doi">10.5256/f1000research.162078.r297337</article-id>
            <title-group>
                <article-title>Reviewer response for version 1</article-title>
            </title-group>
            <contrib-group>
                <contrib contrib-type="author">
                    <name>
                        <surname>Inthasorn</surname>
                        <given-names>Perapong</given-names>
                    </name>
                    <xref ref-type="aff" rid="r297337a1">1</xref>
                    <role>Referee</role>
                    <uri content-type="orcid">https://orcid.org/0000-0002-6617-0307</uri>
                </contrib>
                <aff id="r297337a1">
                    <label>1</label>Faculty of Medicine Siriraj Hospital, Department of Obstetrics &amp; Gynecology, Mahidol University, Bangkok, Thailand</aff>
            </contrib-group>
            <author-notes>
                <fn fn-type="conflict">
                    <p>
                        <bold>Competing interests: </bold>No competing interests were disclosed.</p>
                </fn>
            </author-notes>
            <pub-date pub-type="epub">
                <day>13</day>
                <month>12</month>
                <year>2024</year>
            </pub-date>
            <permissions>
                <copyright-statement>Copyright: &#x00a9; 2024 Inthasorn P</copyright-statement>
                <copyright-year>2024</copyright-year>
                <license xlink:href="https://creativecommons.org/licenses/by/4.0/">
                    <license-p>This is an open access peer review report distributed under the terms of the Creative Commons Attribution Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.</license-p>
                </license>
            </permissions>
            <related-article ext-link-type="doi" id="relatedArticleReport297337" related-article-type="peer-reviewed-article" xlink:href="10.12688/f1000research.147840.1"/>
            <custom-meta-group>
                <custom-meta>
                    <meta-name>recommendation</meta-name>
                    <meta-value>approve-with-reservations</meta-value>
                </custom-meta>
            </custom-meta-group>
        </front-stub>
        <body>
            <p>This is randomized controlled trial compared anxiety level in planned cesarean pregnant women between intervention group(informative VDO watching ) and control group. The study showed that STAI score&#x00a0;of the intervention and control groups did not differ significantly before cesarean delivery. However the postoperative score had significantly decrease in intervention group compared to control group.</p>
            <p> I would like the authors to discuss about this topics.</p>
            <p> 1. Why anxiety did not decrease preoperatively?</p>
            <p> 2.In intervention group, could the anxiety decrease preoperatively, If the subjects in intervention group watched VDO 1 week before cesarean section ?</p>
            <p>Is the work clearly and accurately presented and does it cite the current literature?</p>
            <p>Yes</p>
            <p>If applicable, is the statistical analysis and its interpretation appropriate?</p>
            <p>I cannot comment. A qualified statistician is required.</p>
            <p>Are all the source data underlying the results available to ensure full reproducibility?</p>
            <p>Yes</p>
            <p>Is the study design appropriate and is the work technically sound?</p>
            <p>Yes</p>
            <p>Are the conclusions drawn adequately supported by the results?</p>
            <p>Yes</p>
            <p>Are sufficient details of methods and analysis provided to allow replication by others?</p>
            <p>Yes</p>
            <p>Reviewer Expertise:</p>
            <p>I am obstetrician and gynaecologist specialized in gynaecologic oncologist.</p>
            <p>I confirm that I have read this submission and believe that I have an appropriate level of expertise to confirm that it is of an acceptable scientific standard, however I have significant reservations, as outlined above.</p>
        </body>
        <sub-article article-type="response" id="comment12969-297337">
            <front-stub>
                <contrib-group>
                    <contrib contrib-type="author">
                        <name>
                            <surname>Phetprapasri</surname>
                            <given-names>Panicha</given-names>
                        </name>
                        <aff>Obstetrics and Gynecology, Thammasat University, -, Pathum Thani, Thailand</aff>
                    </contrib>
                </contrib-group>
                <author-notes>
                    <fn fn-type="conflict">
                        <p>
                            <bold>Competing interests: </bold>No competing interests were disclosed.</p>
                    </fn>
                </author-notes>
                <pub-date pub-type="epub">
                    <day>15</day>
                    <month>12</month>
                    <year>2024</year>
                </pub-date>
            </front-stub>
            <body>
                <p>I have carefully reviewed the feedback and have made the required revisions in the "Submit Version 3" section of F1000 Research. Thank you for your guidance and consideration.</p>
            </body>
        </sub-article>
    </sub-article>
    <sub-article article-type="reviewer-report" id="report311443">
        <front-stub>
            <article-id pub-id-type="doi">10.5256/f1000research.162078.r311443</article-id>
            <title-group>
                <article-title>Reviewer response for version 1</article-title>
            </title-group>
            <contrib-group>
                <contrib contrib-type="author">
                    <name>
                        <surname>La Rosa</surname>
                        <given-names>Valentina Lucia</given-names>
                    </name>
                    <xref ref-type="aff" rid="r311443a1">1</xref>
                    <role>Referee</role>
                    <uri content-type="orcid">https://orcid.org/0000-0002-6619-6777</uri>
                </contrib>
                <aff id="r311443a1">
                    <label>1</label>Department of Educational Sciences, University of Catania, Catania, Italy</aff>
            </contrib-group>
            <author-notes>
                <fn fn-type="conflict">
                    <p>
                        <bold>Competing interests: </bold>No competing interests were disclosed.</p>
                </fn>
            </author-notes>
            <pub-date pub-type="epub">
                <day>21</day>
                <month>8</month>
                <year>2024</year>
            </pub-date>
            <permissions>
                <copyright-statement>Copyright: &#x00a9; 2024 La Rosa VL</copyright-statement>
                <copyright-year>2024</copyright-year>
                <license xlink:href="https://creativecommons.org/licenses/by/4.0/">
                    <license-p>This is an open access peer review report distributed under the terms of the Creative Commons Attribution Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.</license-p>
                </license>
            </permissions>
            <related-article ext-link-type="doi" id="relatedArticleReport311443" related-article-type="peer-reviewed-article" xlink:href="10.12688/f1000research.147840.1"/>
            <custom-meta-group>
                <custom-meta>
                    <meta-name>recommendation</meta-name>
                    <meta-value>approve-with-reservations</meta-value>
                </custom-meta>
            </custom-meta-group>
        </front-stub>
        <body>
            <p>The manuscript &#x201c;Effect of Informative Cesarean Delivery Operative Steps Video on Maternal Anxiety Level: A Randomized Controlled Trial&#x201d; provides valuable insights into a practical intervention aimed at reducing maternal anxiety during cesarean delivery. The study is well conducted and presents its findings clearly, but there are several areas where additional detail and discussion are needed to fully contextualize the findings. Below are some comments and suggestions for improving the manuscript:</p>
            <p> </p>
            <p> 
                <bold>Major Revisions</bold> 
                <list list-type="order">
                    <list-item>
                        <p>In the Introduction, I recommend expanding the discussion of the effects of cesarean delivery on maternal mental health, particularly highlighting the differences between planned (elective) cesarean sections and emergency cesarean sections. The psychological effects, including anxiety and postpartum depression, may differ significantly between these two types of procedures and should be further explored. Including a more detailed review of relevant studies [ref 1,2 and 3] would strengthen the background of your study.</p>
                    </list-item>
                    <list-item>
                        <p>The study exclusion criteria appear to be comprehensive, but may potentially limit generalizability. In particular, the exclusion of women with pre-pregnancy mental disorders may introduce selection bias. These women may represent a significant proportion of those who experience anxiety prior to cesarean section. I recommend expanding the discussion of how this limitation affects the generalizability of the study findings.</p>
                    </list-item>
                    <list-item>
                        <p>The STAI was only used to assess anxiety up to postpartum day 1. Anxiety may manifest or change in the later postpartum period due to additional factors such as breastfeeding difficulties or infant care. Consider discussing the rationale for this limited follow-up period and recognize the need for longer-term assessment of maternal anxiety in future research.</p>
                    </list-item>
                    <list-item>
                        <p>The authors should provide more detailed information on the psychometric properties of the State-Trait Anxiety Inventory. Specifically, it would be beneficial to include data on its reliability (e.g., Cronbach's alpha) and validity within the study sample.</p>
                    </list-item>
                    <list-item>
                        <p>Although the manuscript provides a detailed description of the video, more information is needed about its content development. Was the video content tested for comprehension and appropriateness before being used in this study? It would be helpful to include details on how the video was validated or pre-tested for clarity and accuracy.</p>
                    </list-item>
                    <list-item>
                        <p>Although the study enrolled 178 participants, a sample size calculation or power analysis to justify the number of participants is not clearly provided in the text. Please clarify if this was done and include it in the methods section.</p>
                    </list-item>
                    <list-item>
                        <p>The discussion is thorough, but lacks a deeper exploration of how the findings are consistent with or contradict existing literature. The authors should expand on how their findings compare to other studies that have examined similar interventions, particularly those with different outcomes. Discussion of possible mechanisms for the observed reductions in anxiety would strengthen the paper's contribution to the field.</p>
                    </list-item>
                    <list-item>
                        <p>The manuscript mentions ethical approval, but it would benefit from a more detailed discussion of how informed consent was obtained, particularly with regard to how participants were informed about the randomization process and the potential benefits/risks of watching the video.</p>
                    </list-item>
                </list> </p>
            <p> 
                <bold>Minor Revisions</bold> 
                <list list-type="order">
                    <list-item>
                        <p>The manuscript is generally clear, but there are a few areas where language could be improved for clarity. For instance, some sentences in the discussion section are overly complex. Consider simplifying them to improve readability.</p>
                    </list-item>
                </list>
            </p>
            <p>Is the work clearly and accurately presented and does it cite the current literature?</p>
            <p>Partly</p>
            <p>If applicable, is the statistical analysis and its interpretation appropriate?</p>
            <p>Yes</p>
            <p>Are all the source data underlying the results available to ensure full reproducibility?</p>
            <p>Partly</p>
            <p>Is the study design appropriate and is the work technically sound?</p>
            <p>Yes</p>
            <p>Are the conclusions drawn adequately supported by the results?</p>
            <p>Partly</p>
            <p>Are sufficient details of methods and analysis provided to allow replication by others?</p>
            <p>Partly</p>
            <p>Reviewer Expertise:</p>
            <p>Developmental and perinatal psychology</p>
            <p>I confirm that I have read this submission and believe that I have an appropriate level of expertise to confirm that it is of an acceptable scientific standard, however I have significant reservations, as outlined above.</p>
        </body>
        <back>
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                    <label>2</label>
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        </back>
        <sub-article article-type="response" id="comment12396-311443">
            <front-stub>
                <contrib-group>
                    <contrib contrib-type="author">
                        <name>
                            <surname>Phetprapasri</surname>
                            <given-names>Panicha</given-names>
                        </name>
                        <aff>Obstetrics and Gynecology, Thammasat University, -, Pathum Thani, Thailand</aff>
                    </contrib>
                </contrib-group>
                <author-notes>
                    <fn fn-type="conflict">
                        <p>
                            <bold>Competing interests: </bold>No competing interests were disclosed.</p>
                    </fn>
                </author-notes>
                <pub-date pub-type="epub">
                    <day>9</day>
                    <month>9</month>
                    <year>2024</year>
                </pub-date>
            </front-stub>
            <body>
                <p>I have carefully reviewed the feedback and have made the required revisions in the "Submit New Version" section of F1000 Research. Thank you for your guidance and consideration.</p>
            </body>
        </sub-article>
    </sub-article>
</article>
