<?xml version="1.0" encoding="UTF-8"?><!DOCTYPE article PUBLIC "-//NLM//DTD JATS (Z39.96) Journal Publishing DTD v1.2 20190208//EN" "http://jats.nlm.nih.gov/publishing/1.2/JATS-journalpublishing1.dtd"><article xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" article-type="brief-report" dtd-version="1.2" xml:lang="en">
    <front>
        <journal-meta>
            <journal-id journal-id-type="pmc">F1000Research</journal-id>
            <journal-title-group>
                <journal-title>F1000Research</journal-title>
            </journal-title-group>
            <issn pub-type="epub">2046-1402</issn>
            <publisher>
                <publisher-name>F1000 Research Limited</publisher-name>
                <publisher-loc>London, UK</publisher-loc>
            </publisher>
        </journal-meta>
        <article-meta>
            <article-id pub-id-type="doi">10.12688/f1000research.172683.1</article-id>
            <article-categories>
                <subj-group subj-group-type="heading">
                    <subject>Brief Report</subject>
                </subj-group>
                <subj-group>
                    <subject>Articles</subject>
                </subj-group>
            </article-categories>
            <title-group>
                <article-title>THC vape use patterns and device features: Observations from user-posted videos</article-title>
                <fn-group content-type="pub-status">
                    <fn>
                        <p>[version 1; peer review: 1 not approved]</p>
                    </fn>
                </fn-group>
            </title-group>
            <contrib-group>
                <contrib contrib-type="author" corresp="no">
                    <name>
                        <surname>Shihadeh</surname>
                        <given-names>Layla</given-names>
                    </name>
                    <role content-type="http://credit.niso.org/">Conceptualization</role>
                    <role content-type="http://credit.niso.org/">Data Curation</role>
                    <role content-type="http://credit.niso.org/">Formal Analysis</role>
                    <role content-type="http://credit.niso.org/">Methodology</role>
                    <role content-type="http://credit.niso.org/">Visualization</role>
                    <role content-type="http://credit.niso.org/">Writing &#x2013; Original Draft Preparation</role>
                    <role content-type="http://credit.niso.org/">Writing &#x2013; Review &amp; Editing</role>
                    <uri content-type="orcid">https://orcid.org/0009-0004-6341-3475</uri>
                    <xref ref-type="aff" rid="a1">1</xref>
                </contrib>
                <contrib contrib-type="author" corresp="no">
                    <name>
                        <surname>Fares</surname>
                        <given-names>Hadi</given-names>
                    </name>
                    <role content-type="http://credit.niso.org/">Conceptualization</role>
                    <role content-type="http://credit.niso.org/">Data Curation</role>
                    <role content-type="http://credit.niso.org/">Formal Analysis</role>
                    <role content-type="http://credit.niso.org/">Methodology</role>
                    <role content-type="http://credit.niso.org/">Visualization</role>
                    <role content-type="http://credit.niso.org/">Writing &#x2013; Original Draft Preparation</role>
                    <role content-type="http://credit.niso.org/">Writing &#x2013; Review &amp; Editing</role>
                    <uri content-type="orcid">https://orcid.org/0009-0007-1460-5800</uri>
                    <xref ref-type="aff" rid="a1">1</xref>
                </contrib>
                <contrib contrib-type="author" corresp="yes">
                    <name>
                        <surname>El Hourani</surname>
                        <given-names>Mario</given-names>
                    </name>
                    <role content-type="http://credit.niso.org/">Conceptualization</role>
                    <role content-type="http://credit.niso.org/">Data Curation</role>
                    <role content-type="http://credit.niso.org/">Formal Analysis</role>
                    <role content-type="http://credit.niso.org/">Methodology</role>
                    <role content-type="http://credit.niso.org/">Supervision</role>
                    <role content-type="http://credit.niso.org/">Validation</role>
                    <role content-type="http://credit.niso.org/">Visualization</role>
                    <role content-type="http://credit.niso.org/">Writing &#x2013; Original Draft Preparation</role>
                    <role content-type="http://credit.niso.org/">Writing &#x2013; Review &amp; Editing</role>
                    <uri content-type="orcid">https://orcid.org/0000-0001-7176-6175</uri>
                    <xref ref-type="corresp" rid="c1">a</xref>
                    <xref ref-type="aff" rid="a1">1</xref>
                </contrib>
                <aff id="a1">
                    <label>1</label>American University of Beirut, Beirut, Beirut Governorate, Lebanon</aff>
            </contrib-group>
            <author-notes>
                <corresp id="c1">
                    <label>a</label>
                    <email xlink:href="mailto:me217@aub.edu.lb">me217@aub.edu.lb</email>
                </corresp>
                <fn fn-type="conflict">
                    <p>No competing interests were disclosed.</p>
                </fn>
            </author-notes>
            <pub-date pub-type="epub">
                <day>27</day>
                <month>11</month>
                <year>2025</year>
            </pub-date>
            <pub-date pub-type="collection">
                <year>2025</year>
            </pub-date>
            <volume>14</volume>
            <elocation-id>1325</elocation-id>
            <history>
                <date date-type="accepted">
                    <day>20</day>
                    <month>11</month>
                    <year>2025</year>
                </date>
            </history>
            <permissions>
                <copyright-statement>Copyright: &#x00a9; 2025 Shihadeh L et al.</copyright-statement>
                <copyright-year>2025</copyright-year>
                <license xlink:href="https://creativecommons.org/licenses/by/4.0/">
                    <license-p>This is an open access article distributed under the terms of the Creative Commons Attribution Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.</license-p>
                </license>
            </permissions>
            <self-uri content-type="pdf" xlink:href="https://f1000research.com/articles/14-1325/pdf"/>
            <abstract>
                <sec>
                    <title>Background</title>
                    <p>Tetrahydrocannabinol (THC) vaping has become increasingly prevalent, yet available empirical data on puffing behavior during THC vape use remain limited. This study aimed to characterize the puff duration of people who use THC vapes by analyzing publicly available YouTube videos. Additional observations included device type, liquid composition, and notable user behaviors.</p>
                </sec>
                <sec>
                    <title>Methods</title>
                    <p>YouTube was systematically searched using predefined search terms related to THC vaping. Videos published between 2018 and 2024 were screened for inclusion based on clear visibility of puffing events. A total of 100 videos were included in the analysis. Puff initiation and termination were defined by visible facial muscle contraction and relaxation, respectively. Puff duration was determined using frame-by-frame analysis.</p>
                </sec>
                <sec>
                    <title>Results</title>
                    <p>The mean puff duration obtained from 456 analyzed puffs was 3.68 seconds with no significant differences between cartridge and disposable devices (
                        <italic toggle="yes">p</italic> =0.1). Behavioral observations included engagement in extended puffing, coughing, and subjective description of aerosol harshness and flavor.</p>
                </sec>
                <sec>
                    <title>Conclusions</title>
                    <p>The observed puff durations during THC vape use closely aligned with those previously reported for electronic nicotine delivery systems (ENDS), suggesting that ENDS puffing regimens may be appropriate for preliminary emissions testing of THC vape products. However, distinct user behaviors reported in this study highlight the need for THC vape specific protocols that reflect real-word use patterns.</p>
                </sec>
            </abstract>
            <kwd-group kwd-group-type="author">
                <kwd>THC</kwd>
                <kwd>vaping</kwd>
                <kwd>puff duration</kwd>
            </kwd-group>
            <funding-group>
                <funding-statement>The author(s) declared that no grants were involved in supporting this work.</funding-statement>
            </funding-group>
        </article-meta>
    </front>
    <body>
        <sec id="sec5" sec-type="intro">
            <title>Introduction</title>
            <p>The use of tetrahydrocannabinol vapes (THC vapes) has become widespread across diverse populations. For example, past month use prevalence was found to be 12.8% among 13&#x2013;17-year-olds in New England,
                <sup>
                    <xref ref-type="bibr" rid="ref1">1</xref>
                </sup> and 21.3% among electronic nicotine delivery system (ENDS) users aged 11-18 in a US nationally representative sample.
                <sup>
                    <xref ref-type="bibr" rid="ref2">2</xref>
                </sup> This sustained level of THC vaping is particularly notable following the 2019 outbreak of vaping-associated lung injuries, where most affected individuals reported using THC-containing products.
                <sup>
                    <xref ref-type="bibr" rid="ref3">3</xref>
                </sup>
            </p>
            <p>For many years, laboratories have used standardized puffing regimens to assess toxicant emissions from tobacco products.
                <sup>
                    <xref ref-type="bibr" rid="ref4">4</xref>&#x2013;
                    <xref ref-type="bibr" rid="ref6">6</xref>
                </sup> Because toxicant yields are highly sensitive to puffing parameters,
                <sup>
                    <xref ref-type="bibr" rid="ref7">7</xref>,
                    <xref ref-type="bibr" rid="ref8">8</xref>
                </sup> knowledge of device-specific puffing behaviors is essential for accurate emissions testing. However, such information for THC vaping devices remains limited. Emerging studies have either applied ENDS puffing regimens,
                <sup>
                    <xref ref-type="bibr" rid="ref9">9</xref>
                </sup> or used protocols developed for cannabis joints,
                <sup>
                    <xref ref-type="bibr" rid="ref10">10</xref>
                </sup> yet it remains unclear whether these regimens reflect actual use patterns for THC vapes.</p>
            <p>To address this gap, we examined puff topography associated with THC vaping by analyzing publicly posted YouTube videos. We focused primarily on puff duration, while also documenting device types, liquid composition, and notable user behaviors.</p>
        </sec>
        <sec id="sec6" sec-type="methods">
            <title>Methods</title>
            <p>We identified THC vape review videos and extracted information regarding device type, liquid composition, user behaviors, and puff duration.</p>
            <sec id="sec7">
                <title>Video sourcing and screening</title>
                <p>We used the search terms &#x201c;THC e-cigarette review,&#x201d; &#x201c;THC cartridge review,&#x201d; &#x201c;E-cig THC,&#x201d; &#x201c;THC vape unboxing,&#x201d; and &#x201c;THC vape review.&#x201d; Inclusion criteria required videos to feature at least one individual visibly using a THC vaping device and to be published between 2018 and 2024. Videos were excluded if the vaping process was obscured by special effects, altered speeds, or interruptions. If a video featured multiple products, puff duration was analyzed separately for each. When two people used the same device, their data were grouped as a single dataset. We analyzed the first 100 videos that met these criteria.</p>
            </sec>
            <sec id="sec8">
                <title>Puff duration measurement</title>
                <p>Videos were sorted chronologically by sourcing date and analyzed directly on YouTube using a frame-by-frame tool. The frame rate for each video was determined, and the number of frames per puff was converted to seconds.</p>
                <p>Puff initiation was defined by visible facial muscle contraction after device insertion; puff termination was defined by facial muscle relaxation or device removal, whichever occurred first. When devices included a light indicator, its activation coincided with facial contraction, supporting the method&#x2019;s reliability. Consecutive puffs separated by less than 0.5 seconds were combined.</p>
                <p>One research team member measured puff duration for all videos. To assess reliability, a second member, blinded to the first member&#x2019;s results, independently analyzed 25 randomly selected videos.</p>
            </sec>
            <sec id="sec9">
                <title>Other information recorded</title>
                <p>We documented product type and liquid composition, based on visible labels or, when absent, manufacturer or reseller websites located via Google Image Search. During video analysis, we also recorded instances of coughing, double-puffing, and attempts to trigger a device&#x2019;s puff time limiter (i.e., the &#x201c;blinker challenge&#x201d;).</p>
            </sec>
            <sec id="sec10">
                <title>Statistical analysis</title>
                <p>Descriptive statistics (mean, median, interquartile range, minimum, and maximum) were used to summarize puff durations by device type. Independent 
                    <italic toggle="yes">t</italic>-tests were applied to compare cartridge and disposable products, and paired 
                    <italic toggle="yes">t</italic>-tests were used to assess interrater reliability in the reanalyzed subset. Statistical significance was set at 
                    <italic toggle="yes">p</italic> &lt; 0.05.</p>
            </sec>
        </sec>
        <sec id="sec11" sec-type="results">
            <title>Results</title>
            <p>In typical videos, reviewers unboxed and presented the device, took one or more puffs, and described the effects experienced. Devices were either pre-filled cartridges designed for external batteries or disposables combining battery and cartridge (see 
                <xref ref-type="fig" rid="f1">
Figure 1</xref>). Reviewers prioritized describing cannabinoid and terpene composition, with delta-8 THC being most frequently reported. Other THC types mentioned included THCP, THCA, THCO, and delta-10.</p>
            <fig fig-type="figure" id="f1" orientation="portrait" position="float">
                <label>
Figure 1. </label>
                <caption>
                    <title>Representative THC vaping devices observed in the analyzed YouTube videos, categorized as cartridge-based systems requiring external batteries (left) and disposable all-in-one units (right).</title>
                </caption>
                <graphic id="gr1" orientation="portrait" position="float" xlink:href="https://f1000research-files.f1000.com/manuscripts/190426/a7b12b3e-7bc7-452d-92a5-03bd7a190839_figure1.gif"/>
            </fig>
            <p>Presenters often took short, rapid succession clearing puffs before inhalation. In disposable devices, several attempted the &#x201c;blinker challenge,&#x201d; drawing until the device&#x2019;s LED light blinked to signal the puff duration limit. They frequently commented on vapor harshness and expressed a preference for smooth aerosols, describing flavors as &#x201c;sweet,&#x201d; &#x201c;earthy,&#x201d; or &#x201c;cannabis-like.&#x201d; Coughing after inhalation was common. After vaping, presenters assessed the intensity and onset speed of the psychoactive effects. Of the 98 individuals observed, 87 appeared to be male.</p>
            <p>Across the 100 videos, 456 puffs were analyzed. Puff durations ranged from 0.12 to 18.01 seconds, with a mean of 3.68 seconds. The 25th percentile puff duration was 1.85 seconds, the median was 2.94 seconds, and the 75th percentile was 4.39 seconds. In the subset of 25 randomly selected videos, the second rater recorded a mean puff duration of 3.85 seconds compared to 3.68 seconds for the original rater; paired t-test analysis showed no statistically significant difference (
                <italic toggle="yes">p</italic> = 0.7). 
                <xref ref-type="table" rid="T1">
Table 1</xref> summarizes puff durations by device type. No statistically significant difference in puff duration was found between cartridge and disposable products (
                <italic toggle="yes">p</italic> = 0.1). The raw data supporting these findings, including puff duration measurements from 100 publicly available YouTube videos and the reanalysis of 25 randomly selected videos by a second rater to assess measurement reliability, are available in the Mendeley Data repository.
                <sup>
                    <xref ref-type="bibr" rid="ref11">11</xref>
                </sup>
            </p>
            <table-wrap id="T1" orientation="portrait" position="float">
                <label>
Table 1. </label>
                <caption>
                    <title>Measured puff duration (sec) using video frame analysis.</title>
                    <p>Q1: first quartile (25th percentile); Q3: third quartile (75th percentile).</p>
                </caption>
                <table content-type="article-table" frame="hsides">
                    <thead>
                        <tr>
                            <th align="left" colspan="1" rowspan="1" valign="top"/>
                            <th align="left" colspan="1" rowspan="1" valign="top">Cartridge</th>
                            <th align="left" colspan="1" rowspan="1" valign="top">Disposable</th>
                            <th align="left" colspan="1" rowspan="1" valign="top">
All products</th>
                        </tr>
                    </thead>
                    <tbody>
                        <tr>
                            <td align="left" colspan="1" rowspan="1" valign="top">
                                <bold>N</bold>
</td>
                            <td align="left" colspan="1" rowspan="1" valign="top">58</td>
                            <td align="left" colspan="1" rowspan="1" valign="top">42</td>
                            <td align="left" colspan="1" rowspan="1" valign="top">100</td>
                        </tr>
                        <tr>
                            <td align="left" colspan="1" rowspan="1" valign="top">
                                <bold>Average</bold>
</td>
                            <td align="left" colspan="1" rowspan="1" valign="top">3.87</td>
                            <td align="left" colspan="1" rowspan="1" valign="top">3.45</td>
                            <td align="left" colspan="1" rowspan="1" valign="top">3.68</td>
                        </tr>
                        <tr>
                            <td align="left" colspan="1" rowspan="1" valign="top">
                                <bold>Median</bold>
</td>
                            <td align="left" colspan="1" rowspan="1" valign="top">3.23</td>
                            <td align="left" colspan="1" rowspan="1" valign="top">2.69</td>
                            <td align="left" colspan="1" rowspan="1" valign="top">2.94</td>
                        </tr>
                        <tr>
                            <td align="left" colspan="1" rowspan="1" valign="top">
                                <bold>Q1</bold>
</td>
                            <td align="left" colspan="1" rowspan="1" valign="top">2.06</td>
                            <td align="left" colspan="1" rowspan="1" valign="top">1.63</td>
                            <td align="left" colspan="1" rowspan="1" valign="top">1.85</td>
                        </tr>
                        <tr>
                            <td align="left" colspan="1" rowspan="1" valign="top">
                                <bold>Q3</bold>
</td>
                            <td align="left" colspan="1" rowspan="1" valign="top">4.43</td>
                            <td align="left" colspan="1" rowspan="1" valign="top">4.35</td>
                            <td align="left" colspan="1" rowspan="1" valign="top">4.39</td>
                        </tr>
                        <tr>
                            <td align="left" colspan="1" rowspan="1" valign="top">
                                <bold>Minimum</bold>
</td>
                            <td align="left" colspan="1" rowspan="1" valign="top">0.12</td>
                            <td align="left" colspan="1" rowspan="1" valign="top">0.57</td>
                            <td align="left" colspan="1" rowspan="1" valign="top">0.12</td>
                        </tr>
                        <tr>
                            <td align="left" colspan="1" rowspan="1" valign="top">
                                <bold>Maximum</bold>
</td>
                            <td align="left" colspan="1" rowspan="1" valign="top">18.01</td>
                            <td align="left" colspan="1" rowspan="1" valign="top">12</td>
                            <td align="left" colspan="1" rowspan="1" valign="top">18.01</td>
                        </tr>
                    </tbody>
                </table>
            </table-wrap>
        </sec>
        <sec id="sec12" sec-type="discussion">
            <title>Discussion</title>
            <p>This study was conducted to address the lack of empirical data regarding puff duration during the use of cannabis vaping products, a critical factor for laboratory toxicant emissions testing. A secondary aim was to explore product attributes valued by users. By analyzing 100 YouTube videos of cannabis vape product reviews posted between 2018 and 2024, we were able to characterize puff topography and user preferences in a real-world context.</p>
            <p>Our findings revealed a mean puff duration of 3.68 &#x00b1; 2.66 seconds across 456 puffs. These results align closely with prior research.
                <sup>
                    <xref ref-type="bibr" rid="ref12">12</xref>
                </sup> Self-reported puff duration data from an online survey completed by 557 respondents (75% identified as male), with a mean puff duration of 5.1 &#x00b1; 2.5 seconds among the 293 individuals who vaped cannabis liquids. In a separate study, Smith et al. (2024)
                <sup>
                    <xref ref-type="bibr" rid="ref13">13</xref>
                </sup> directly measured puff topography from 9 participants over two weeks using a cannabis oil vaping device (Gram1&#x2122;) equipped with an integrated puff counter and puff duration sensor, finding individual mean puff durations ranging from 2.20 to 6.95 seconds, with a population mean of 4.06 seconds. Importantly, the mean puff duration identified in our study is also comparable to those documented for users of electronic nicotine delivery systems (ENDS). For instance, Hua et al. (2013)
                <sup>
                    <xref ref-type="bibr" rid="ref14">14</xref>
                </sup> reported a mean puff duration of 4.3 &#x00b1; 1.5 seconds in a sample of 64 individuals, and clinical studies of ENDS users have documented mean durations between 3.5 &#x00b1; 1.4 seconds
                <sup>
                    <xref ref-type="bibr" rid="ref15">15</xref>
                </sup> and 4.16 &#x00b1; 1.06 seconds.
                <sup>
                    <xref ref-type="bibr" rid="ref16">16</xref>
                </sup>
            </p>
            <p>These similarities suggest that existing ENDS puffing regimens may offer a reasonable initial model for laboratory emissions testing of THC vaping products. However, further research tailored specifically to cannabis vapes is warranted to confirm this applicability, given differences in device design, liquid composition, and pharmacology.</p>
            <p>A notable limitation of this study is the underrepresentation of female users among the video presenters: 87 of the 98 individuals appeared male. This demographic imbalance contrasts with recent survey data showing comparable past-month THC vape use rates between women (39.9%) and men (38.1%),
                <sup>
                    <xref ref-type="bibr" rid="ref17">17</xref>
                </sup> suggesting limits to generalizability. Additional limitations include potential selection bias inherent to analyzing public video content, variability in video quality, and reliance on visual indicators of puff events.</p>
            <p>Qualitative observations from the video analyses provided further insights into user priorities. Presenters frequently emphasized the importance of flavor characteristics, throat hit smoothness, and the rapid onset and intensity of pharmacological effects. Additionally, the &#x201c;blinker challenge,&#x201d; in which users attempt to trigger a device&#x2019;s maximum puff duration limit, emerged as a common behavioral feature. This suggests that device features may function similarly to &#x201c;Easter eggs&#x201d;, hidden features that reward users with a sense of achievement for longer puffs and, consequently, higher drawn doses. This phenomenon warrants further investigation given its potential implications for user exposure and behavior.</p>
            <p>In conclusion, this study contributes empirical data on THC vaping behaviors and emphasizes the need for laboratory protocols that accurately reflect real-world usage patterns.</p>
        </sec>
        <sec id="sec13">
            <title>Ethical considerations</title>
            <p>This study did not involve human participants recruitment, or identifiable private information and therefore did not require ethical approval or informed consent. All data were obtained from publicly accessible YouTube videos that are freely available for viewing without restriction. No personal or identifiable information about individuals was collected, recorded, or analyzed. The study adhered to the ethical principles of using publicly available data for research and complies with F1000Research editorial policies.</p>
            <sec id="sec14">
                <title>Reporting guidelines</title>
                <p>Mendeley Data. 
                    <italic toggle="yes">STROBE checklist for &#x2018;THC vape use patterns and device features: Insights from user-posted videos&#x2019;.</italic> DOI: 10.17632/sbzz2hj3xb.1. 
                    <ext-link ext-link-type="uri" xlink:href="https://data.mendeley.com/datasets/sbzz2hj3xb/1">https://data.mendeley.com/datasets/sbzz2hj3xb/1</ext-link>.
                    <sup>
                        <xref ref-type="bibr" rid="ref18">18</xref>
                    </sup>
                </p>
                <p>Data are available under the terms of the 
                    <ext-link ext-link-type="uri" xlink:href="http://creativecommons.org/publicdomain/zero/1.0/">Creative Commons Zero &#x201c;No rights reserved&#x201d; data waiver</ext-link> (CC0 1.0 Public domain dedication).</p>
            </sec>
        </sec>
    </body>
    <back>
        <sec id="sec17" sec-type="data-availability">
            <title>Data availability</title>
            <p>Mendeley Data: THC_VAPE_PUFF_DURATION. DOI: 10.17632/gx856tzfdr.3 
                <ext-link ext-link-type="uri" xlink:href="https://data.mendeley.com/datasets/gx856tzfdr/3">https://data.mendeley.com/datasets/gx856tzfdr/3</ext-link>.
                <sup>
                    <xref ref-type="bibr" rid="ref11">11</xref>
                </sup>
            </p>
            <p>This project contains the following underlying data:
                <list list-type="bullet">
                    <list-item>
                        <label>&#x2022;</label>
                        <p>

                            <italic toggle="yes">THC_Puff Duration_Raw.xlsx</italic> (Puff duration data extracted from 100 publicly available YouTube videos of THC vape use, including search keywords, device type, upload year, and puff duration.)</p>
                    </list-item>
                    <list-item>
                        <label>&#x2022;</label>
                        <p>

                            <italic toggle="yes">THC_Puff Duration_Reanalyzed.xlsx</italic> (Subset of 25 randomly selected videos independently reanalyzed by a second rater to assess measurement reliability, including the percent difference in average puff duration between raters.)</p>
                    </list-item>
                </list>
            </p>
            <p>List of videos available upon reasonable request. Researchers interested in accessing the video list may contact the corresponding author at 
                <email xlink:href="mailto:me217@aub.edu.lb">me217@aub.edu.lb</email>. Requests must include a brief description of the intended use and will be granted provided that they align with the ethical requirements governing the study.</p>
            <p>Data are available under the terms of the 
                <ext-link ext-link-type="uri" xlink:href="https://creativecommons.org/licenses/by/4.0/">Creative Commons Attribution 4.0 International license</ext-link> (CC-BY 4.0).</p>
        </sec>
        <ref-list>
            <title>References</title>
            <ref id="ref1">
                <label>1</label>
                <mixed-citation publication-type="journal">
                    <person-group person-group-type="author">

                        <name name-style="western">
                            <surname>Liu</surname>
                            <given-names>J</given-names>
                        </name>

                        <name name-style="western">
                            <surname>Winickoff</surname>
                            <given-names>JP</given-names>
                        </name>

                        <name name-style="western">
                            <surname>Hanby</surname>
                            <given-names>E</given-names>
                        </name>

                        <etal/>
</person-group>:
                    <article-title>Prevalence and correlates of past 30-day dual-vaping of nicotine and cannabis among adolescents in five New England states.</article-title>
                    <source>

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    <sub-article article-type="reviewer-report" id="report440304">
        <front-stub>
            <article-id pub-id-type="doi">10.5256/f1000research.190426.r440304</article-id>
            <title-group>
                <article-title>Reviewer response for version 1</article-title>
            </title-group>
            <contrib-group>
                <contrib contrib-type="author">
                    <name>
                        <surname>lallai</surname>
                        <given-names>Valeria</given-names>
                    </name>
                    <xref ref-type="aff" rid="r440304a1">1</xref>
                    <role>Referee</role>
                </contrib>
                <aff id="r440304a1">
                    <label>1</label>University of California Irvine, Irvine, California, USA</aff>
            </contrib-group>
            <author-notes>
                <fn fn-type="conflict">
                    <p>
                        <bold>Competing interests: </bold>No competing interests were disclosed.</p>
                </fn>
            </author-notes>
            <pub-date pub-type="epub">
                <day>2</day>
                <month>1</month>
                <year>2026</year>
            </pub-date>
            <permissions>
                <copyright-statement>Copyright: &#x00a9; 2026 lallai V</copyright-statement>
                <copyright-year>2026</copyright-year>
                <license xlink:href="https://creativecommons.org/licenses/by/4.0/">
                    <license-p>This is an open access peer review report distributed under the terms of the Creative Commons Attribution Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.</license-p>
                </license>
            </permissions>
            <related-article ext-link-type="doi" id="relatedArticleReport440304" related-article-type="peer-reviewed-article" xlink:href="10.12688/f1000research.172683.1"/>
            <custom-meta-group>
                <custom-meta>
                    <meta-name>recommendation</meta-name>
                    <meta-value>reject</meta-value>
                </custom-meta>
            </custom-meta-group>
        </front-stub>
        <body>
            <p>The authors examine 100 publicly available videos showing one or more individuals vaping THC to analyze puff duration and related behaviors. The idea is interesting, especially given the need for more consistency across clinical and preclinical studies to better understand THC vaping effects. However, the enthusiasm for the manuscript is limited. It is very concise, and the Introduction does not sufficiently prepare the reader to understand the study&#x2019;s rationale or interpret the results. It lacks enough background to contextualize the research question. In the Methods, it&#x2019;s unclear why data from two individuals using the same device are combined into one dataset. This raises questions about individual differences: is subject variability not relevant to behaviors like puff duration and quality? More justification is needed here. Table 1 compares two values at the 25th percentile, but the choice of this analysis method is unclear, and its justification in the context of standard statistical practice is not explained. Additional clarification is needed to understand the rationale and meaning of these analyses. The Discussion is very brief, and the second paragraph contains content that seems more suitable for the Results section, which itself is very limited. Consequently, the manuscript lacks substantial empirical data on THC vaping behavior, despite the implications of the title. Overall, while the study does not provide new empirical insights into THC vaping, it highlights the importance of developing laboratory protocols that more accurately reflect real-world usage.</p>
            <p>Is the work clearly and accurately presented and does it cite the current literature?</p>
            <p>No</p>
            <p>If applicable, is the statistical analysis and its interpretation appropriate?</p>
            <p>I cannot comment. A qualified statistician is required.</p>
            <p>Are all the source data underlying the results available to ensure full reproducibility?</p>
            <p>Partly</p>
            <p>Is the study design appropriate and is the work technically sound?</p>
            <p>Partly</p>
            <p>Are the conclusions drawn adequately supported by the results?</p>
            <p>Partly</p>
            <p>Are sufficient details of methods and analysis provided to allow replication by others?</p>
            <p>No</p>
            <p>Reviewer Expertise:</p>
            <p>Neurobiology and Behavior, addiction, basic science, animal models, neuroscience</p>
            <p>I confirm that I have read this submission and believe that I have an appropriate level of expertise to state that I do not consider it to be of an acceptable scientific standard, for reasons outlined above.</p>
        </body>
    </sub-article>
</article>
