<?xml version="1.0" encoding="UTF-8"?><!DOCTYPE article PUBLIC "-//NLM//DTD JATS (Z39.96) Journal Publishing DTD v1.2 20190208//EN" "http://jats.nlm.nih.gov/publishing/1.2/JATS-journalpublishing1.dtd"><article xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" article-type="other" dtd-version="1.2" xml:lang="en">
    <front>
        <journal-meta>
            <journal-id journal-id-type="pmc">F1000Research</journal-id>
            <journal-title-group>
                <journal-title>F1000Research</journal-title>
            </journal-title-group>
            <issn pub-type="epub">2046-1402</issn>
            <publisher>
                <publisher-name>F1000 Research Limited</publisher-name>
                <publisher-loc>London, UK</publisher-loc>
            </publisher>
        </journal-meta>
        <article-meta>
            <article-id pub-id-type="doi">10.12688/f1000research.162989.1</article-id>
            <article-categories>
                <subj-group subj-group-type="heading">
                    <subject>Case Study</subject>
                </subj-group>
                <subj-group>
                    <subject>Articles</subject>
                </subj-group>
            </article-categories>
            <title-group>
                <article-title>Evaluating Mycelium as an insulation material: A comparative study on thermal performance, comfort, and energy efficiency</article-title>
                <fn-group content-type="pub-status">
                    <fn>
                        <p>[version 1; peer review: 2 approved with reservations]</p>
                    </fn>
                </fn-group>
            </title-group>
            <contrib-group>
                <contrib contrib-type="author" corresp="yes">
                    <name>
                        <surname>Khaled</surname>
                        <given-names>Bassant</given-names>
                    </name>
                    <role content-type="http://credit.niso.org/">Resources</role>
                    <role content-type="http://credit.niso.org/">Visualization</role>
                    <role content-type="http://credit.niso.org/">Writing &#x2013; Original Draft Preparation</role>
                    <uri content-type="orcid">https://orcid.org/0009-0000-2689-2412</uri>
                    <xref ref-type="corresp" rid="c1">a</xref>
                    <xref ref-type="aff" rid="a1">1</xref>
                </contrib>
                <contrib contrib-type="author" corresp="no">
                    <name>
                        <surname>Hany</surname>
                        <given-names>Nermine</given-names>
                    </name>
                    <role content-type="http://credit.niso.org/">Supervision</role>
                    <role content-type="http://credit.niso.org/">Writing &#x2013; Review &amp; Editing</role>
                    <uri content-type="orcid">https://orcid.org/0000-0003-4816-3127</uri>
                    <xref ref-type="aff" rid="a1">1</xref>
                </contrib>
                <contrib contrib-type="author" corresp="no">
                    <name>
                        <surname>Mosaad</surname>
                        <given-names>Gihan</given-names>
                    </name>
                    <role content-type="http://credit.niso.org/">Supervision</role>
                    <xref ref-type="aff" rid="a1">1</xref>
                </contrib>
                <aff id="a1">
                    <label>1</label>Department of Architectural and Engineering Environmental Design, Arab Academy for Science Technology and Maritime Transport, Alexandria Governorate, 1029, Egypt</aff>
            </contrib-group>
            <author-notes>
                <corresp id="c1">
                    <label>a</label>
                    <email xlink:href="mailto:basent.khaled@yahoo.com">basent.khaled@yahoo.com</email>
                </corresp>
                <fn fn-type="conflict">
                    <p>No competing interests were disclosed.</p>
                </fn>
            </author-notes>
            <pub-date pub-type="epub">
                <day>24</day>
                <month>4</month>
                <year>2025</year>
            </pub-date>
            <pub-date pub-type="collection">
                <year>2025</year>
            </pub-date>
            <volume>14</volume>
            <elocation-id>459</elocation-id>
            <history>
                <date date-type="accepted">
                    <day>17</day>
                    <month>4</month>
                    <year>2025</year>
                </date>
            </history>
            <permissions>
                <copyright-statement>Copyright: &#x00a9; 2025 Khaled B et al.</copyright-statement>
                <copyright-year>2025</copyright-year>
                <license xlink:href="https://creativecommons.org/licenses/by/4.0/">
                    <license-p>This is an open access article distributed under the terms of the Creative Commons Attribution Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.</license-p>
                </license>
            </permissions>
            <self-uri content-type="pdf" xlink:href="https://f1000research.com/articles/14-459/pdf"/>
            <abstract>
                <sec>
                    <title>Background</title>
                    <p>Since the building sector contributes significantly to carbon emissions, using sustainable materials is more crucial than ever. Mycelium is investigated in this study as a natural substitute for conventional insulation materials like rock wool and XPS. Its thermal performance has been evaluated in a residential building in New Cairo, Egypt, using Design-Builder simulations, paying particular attention to U-values, discomfort hours, PPD-PMV, and energy consumption. The results demonstrate that mycelium is environmentally safe, biodegradable, and provides insulation that is comparable to XPS. This demonstrates its promise as a sustainable option for building in the future.</p>
                </sec>
                <sec>
                    <title>Methods</title>
                    <p>This research uses Design-Builder software version 7.3.0.046 integrated with Energy-Plus to simulate the thermal conductivity of mycelium compared to two of the most frequently utilized traditional materials for insulation in Egypt, in Janna Compound, New Cairo. The analysis examines energy consumption, thermal comfort, Predicted Mean Vote (PMV), and Predicted Percentage of Dissatisfaction (PPD%). The study provides a comparative evaluation of mycelium insulation compared to traditional materials like XPS and Rockwool.</p>
                </sec>
                <sec>
                    <title>Results</title>
                    <p>According to the simulation results, mycelium insulation performs comparably to XPS regarding U-values, discomfort hours, PPD-PMV, and energy consumption. Specifically, mycelium achieved a 0.323 U-value, reduced discomfort hours percentage to 16.9%, and achieved a ratio of energy reduction of 15.8% compared to the base case. These results demonstrate how mycelium has the potential to compete with traditional insulation materials while offering significant sustainability advantages, such as biodegradability and a lower carbon footprint.</p>
                </sec>
                <sec>
                    <title>Conclusion</title>
                    <p>In Janna Compound, New Cairo, the research shows how mycelium insulation can improve thermal comfort and save energy usage. It performs similarly to XPS but has more positive environmental effects. These results support the integration of mycelium into Egypt&#x2019;s sustainable housing practices, providing key insights for architects, developers, and policymakers focused on energy-efficient and sustainable urban development.</p>
                </sec>
            </abstract>
            <kwd-group kwd-group-type="author">
                <kwd>Mycelium Insulation</kwd>
                <kwd>sustainable building material</kwd>
                <kwd>energy efficiency</kwd>
                <kwd>thermal comfort</kwd>
            </kwd-group>
            <funding-group>
                <funding-statement>The author(s) declared that no grants were involved in supporting this work.</funding-statement>
            </funding-group>
        </article-meta>
    </front>
    <body>
        <sec id="sec5" sec-type="intro">
            <title>Introduction</title>
            <p>The main concern nowadays worldwide is the problem of energy consumption in building sectors, which approximately reaches 40% of the total energy used. In Egypt over 50% of energy consumption is for residential buildings.
                <sup>
                    <xref ref-type="bibr" rid="ref1">1</xref>
                </sup> As energy demand continues to rise, finding sustainable solutions to lower energy consumption in buildings has become critical.
                <sup>
                    <xref ref-type="bibr" rid="ref2">2</xref>
                </sup> This research investigates how mycelium insulation could improve thermal efficiency. and lower energy usage in residential buildings. Mycelium is an organic degradable material that offers a promising alternative to toxic traditional insulation materials that emit CO
                <sub>2</sub>. For the simulation, the Janna compound in New Cairo, a project done by the Egyptian government in many parts of Egypt, was selected. Aiming to provide sustainable material that line with Egypt&#x2019;s Vision 2030 goals.</p>
            <sec id="sec6">
                <title>Research problem</title>
                <p>Worldwide, the construction sector stands as a major energy user, responsible for consuming more than 40% of the total energy supply, with an average annual increase of 1.5% from 2012 to 2040.
                    <sup>
                        <xref ref-type="bibr" rid="ref1">1</xref>
                    </sup> In Egypt, due to the rapid growth and higher living standards, residential buildings consume over half of the country&#x2019;s electricity
                    <sup>
                        <xref ref-type="bibr" rid="ref1">1</xref>
                    </sup> as shown in 
                    <xref ref-type="fig" rid="f1">
Figure 1</xref>.</p>
                <fig fig-type="figure" id="f1" orientation="portrait" position="float">
                    <label>
Figure 1. </label>
                    <caption>
                        <title>Egypt energy consumption graph.</title>
                    </caption>
                    <graphic id="gr1" orientation="portrait" position="float" xlink:href="https://f1000research-files.f1000.com/manuscripts/179279/ba434910-9183-4f26-a6de-859c894fa5ae_figure1.gif"/>
                </fig>
                <p>Each year in the housing sector, energy use increased by 7%, and without finding a solution, the consumption could be more than double by 2030, increasing from 60 to 135 million tons of oil equivalent (MTOE).
                    <sup>
                        <xref ref-type="bibr" rid="ref2">2</xref>
                    </sup> This problem not only strains energy resources but also increases the environmental challenges, making energy efficiency a critical priority.</p>
                <p>One of the main approaches to reducing energy consumption in the building sector and to meet with Egypt&#x2019;s Vision 2030, is through the improvement of insulation materials. Studies show that enhancing insulation in walls, roofs, and windows could decrease CO
                    <sub>2</sub> emissions by 2190 kilotons per year and save up to 842MW of peak energy demand.
                    <sup>
                        <xref ref-type="bibr" rid="ref1">1</xref>
                    </sup> Alongside decreasing energy consumption, this approach safeguards the planet while promoting healthier and more sustainable living conditions for people.</p>
                <p>This research explores mycelium as a sustainable and circular insulation material. It first examines its properties and potential as an alternative to conventional insulation. Then, it compares mycelium to commonly used materials in Egypt, such as XPS and Rockwool. Finally, a simulation on Janna Compound analyses four scenarios, evaluating energy consumption, discomfort hours, PMV, and PPD to assess mycelium&#x2019;s feasibility in building insulation.</p>
            </sec>
            <sec id="sec7">
                <title>Research aim</title>
                <p>The purpose of this research is to increase academics&#x2019; and designers&#x2019; awareness of the benefits and potential of mycelium insulation. It investigates how well mycelium insulation performs in comparison to two commonly used insulation materials and proposes it to be a sustainable alternative for the construction sector in Egypt.</p>
            </sec>
        </sec>
        <sec id="sec8" sec-type="methods">
            <title>Methods</title>
            <p>The research focuses on examining the performance of mycelium insulation, utilizing a residential building prototype in a typical floor of a type A building within Janna Compound as the case study, located in New Cairo, Egypt. A literature review and a base case study are the two primary elements of the research.</p>
            <sec id="sec9">
                <title>Literature review</title>
                <p>The literature review will address several important subjects, including the definition of mycelium, and explore its sustainability and circularity as a material. It will also illustrate its thermal conductivity properties, which are essential for evaluating its thermal performance as an insulation material. Following this, the review will provide an overview of two traditional insulation materials that are commonly used in Egypt, XPS, and Rockwool.</p>
            </sec>
            <sec id="sec10">
                <title>Base case study</title>
                <p>The research&#x2019;s second section examines a case study of a typical floor in a Type A building within Janna Compound, New Cairo. This simulates how insulating materials affect thermal comfort and energy usage, including Predicted Mean Vote (PMV), Predicted Percentage of Dissatisfaction (PPD%), and thermal comfort hours. The primary aim is to assess how well mycelium insulation performs in comparison to commonly used materials like XPS and Rockwool, to evaluate its usefulness. The scenarios evaluated are:
                    <list list-type="bullet">
                        <list-item>
                            <label>&#x2022;</label>
                            <p>

                                <bold>Baseline model:</bold> Wall without insulation.</p>
                        </list-item>
                        <list-item>
                            <label>&#x2022;</label>
                            <p>

                                <bold>Scenario 1:</bold> Wall insulated with mycelium.</p>
                        </list-item>
                        <list-item>
                            <label>&#x2022;</label>
                            <p>

                                <bold>Scenario 2:</bold> Wall insulated with XPS.</p>
                        </list-item>
                        <list-item>
                            <label>&#x2022;</label>
                            <p>

                                <bold>Scenario 3:</bold> Wall insulated with Rockwool.</p>
                        </list-item>
                    </list>
                </p>
                <p>The investigation&#x2019;s methodology evaluates mycelium&#x2019;s efficacy in comparison to two commonly used materials to improve residential buildings&#x2019; thermal comfort in Egypt. 
                    <ext-link ext-link-type="uri" xlink:href="https://designbuilder.co.uk/">Design-Builder version 7.3.0.046</ext-link> &amp; 
                    <ext-link ext-link-type="uri" xlink:href="https://energyplus.net/">Energy-Plus
</ext-link> plugin software were used for this simulation, which will evaluate discomfort hours, PMV-PPD, and energy consumption for a typical floor in a Type A building in Janna Compound, emphasizing how these materials and techniques affect the thermal condition.</p>
            </sec>
        </sec>
        <sec id="sec11">
            <title>Literature review</title>
            <p>Mycelium is the visible reproductive system of fungi, which is the mushroom fruit, a woven-like network that grows under the ground called mycelium. Mycelium is made from a network of delicate, white filaments measuring between 1 and 30 micrometers in diameter
                <sup>
                    <xref ref-type="bibr" rid="ref3">3</xref>
                </sup> as shown in 
                <xref ref-type="fig" rid="f2">
Figure 2</xref>.</p>
            <fig fig-type="figure" id="f2" orientation="portrait" position="float">
                <label>
Figure 2. </label>
                <caption>
                    <title>The fungi split into underground mycelium and a fruiting body, created using AI tools and modified in Photoshop.</title>
                </caption>
                <graphic id="gr2" orientation="portrait" position="float" xlink:href="https://f1000research-files.f1000.com/manuscripts/179279/ba434910-9183-4f26-a6de-859c894fa5ae_figure2.gif"/>
            </fig>
            <p>In recent years, mycelium has gained a lot of attention from academic and industrial sectors because, during growth, it requires a minimal amount of energy, lacks by-product generation, and has multiple potential uses.
                <sup>
                    <xref ref-type="bibr" rid="ref4">4</xref>
                </sup>
            </p>
            <p>Indoor air quality was enhanced by using mycelium, due to its natural air-purifying properties, especially in filtering particulate matter (PM). Studies have demonstrated its effectiveness due to its breathable structure, which helps capture and remove airborne such as PM2.5 and PM10. Leading to improving air purity.
                <sup>
                    <xref ref-type="bibr" rid="ref5">5</xref>
                </sup>
            </p>
            <p>Mycelium&#x2019;s inherent properties allow it to regulate indoor moisture levels effectively, absorbing and maintaining optimal humidity for a more comfortable indoor environment. Additionally, mycelium has the capability to capture and store over 16 metric tons of massive amounts of carbon from the atmosphere in just one month.
                <sup>
                    <xref ref-type="bibr" rid="ref6">6</xref>
                </sup> Mycelium-based materials have a variety of applications across various industries, they can be utilized as acoustic panels, tiles, bricks, and furniture due to their lightweight and permeable structure, as well as their thermal, fire, and water resistance, acoustic absorption capabilities, and aesthetic appeal.
                <sup>
                    <xref ref-type="bibr" rid="ref7">7</xref>
                </sup>
            </p>
            <sec id="sec12">
                <title>Sustainability and circularity of mycelium material</title>
                <p>The environmentally friendly characteristics of mycelium-based materials and their potential role in shaping the future of sustainable construction. Six principal factors support construction
                    <sup>
                        <xref ref-type="bibr" rid="ref8">8</xref>
                    </sup> as shown in 
                    <xref ref-type="fig" rid="f3">
Figure 3</xref>.</p>
                <fig fig-type="figure" id="f3" orientation="portrait" position="float">
                    <label>
Figure 3. </label>
                    <caption>
                        <title>The sustainable life cycle of materials sourced from mycelium.</title>
                    </caption>
                    <graphic id="gr3" orientation="portrait" position="float" xlink:href="https://f1000research-files.f1000.com/manuscripts/179279/ba434910-9183-4f26-a6de-859c894fa5ae_figure3.gif"/>
                </fig>
                <p>&#x2003;&#x2003;-Cost-effectiveness and abundant raw materials&#x2003;&#x2003;&#x2003;-Biodegradability</p>
                <p>&#x2003;&#x2003;-Rapid manufacturing process&#x2003;&#x2003;&#x2003;&#x2003;&#x2003;&#x2003;&#x2003;&#x2003;&#x2003;&#x2003;-Flexibility</p>
                <p>&#x2003;&#x2003;-Minimal Energy consumption&#x2003;&#x2003;&#x2003;&#x2003;&#x2003;&#x2003;&#x2003;&#x2003;&#x2003;-Cardle-to-cradle life cycle</p>
            </sec>
            <sec id="sec13">
                <title>Thermal and physical properties of mycelium insulation material</title>
                <p>Heat transfer, heat storage capacity, material density, and thermal diffusivity are among the thermal and physical attributes of insulation materials used in the construction sector. These features define a material&#x2019;s efficiency in terms of heat absorption, transport, and retention. When combined, these characteristics help a structure use less energy and maintain a pleasant interior temperature, which lowers the need for artificial heating or cooling.
                    <sup>
                        <xref ref-type="bibr" rid="ref9">9</xref>
                    </sup>
                </p>
                <p>When combining mycelium with agricultural residences, it stands out as a promising alternative to foam insulation materials. This enables the formation of porous composites, such as foams, to be formed. Due to their higher moisture content, the samples show a higher Heat transfer rate.
                    <sup>
                        <xref ref-type="bibr" rid="ref10">10</xref>
                    </sup> Thermal conductivity refers to how efficiently a material transfers heat; lower values indicate better insulation performance. Research on Mycelium-based composites suggests that their thermal conductivity ranges between 0.029-0.104 W/mK, making them a good option for insulation.
                    <sup>
                        <xref ref-type="bibr" rid="ref11">11</xref>
                    </sup>
                </p>
                <p>
                    <xref ref-type="table" rid="T1">
Table 1</xref> shows the characteristics of the mycelium insulation panel made by BIOHM
                    <sup>&#x00ae;</sup> (
                    <ext-link ext-link-type="uri" xlink:href="https://www.biohm.co.uk/">https://www.biohm.co.uk/</ext-link>), which is used in the simulation, the panel dimension is 1.2&#x00d7;2.4m, with a thickness of 0.075m.
                    <sup>
                        <xref ref-type="bibr" rid="ref12">12</xref>
                    </sup>
                </p>
                <table-wrap id="T1" orientation="portrait" position="float">
                    <label>
Table 1. </label>
                    <caption>
                        <title>Performance characteristics of the Mycelium Insulation panel used in the simulation.
                            <sup>
                                <xref ref-type="bibr" rid="ref12">12</xref>
                            </sup>
                        </title>
                    </caption>
                    <table content-type="article-table" frame="hsides">
                        <thead>
                            <tr>
                                <th align="left" colspan="1" rowspan="1" valign="top">
Insulation material</th>
                                <th align="left" colspan="1" rowspan="1" valign="top">
Density (kg/m
                                    <sup>3</sup>)</th>
                                <th align="left" colspan="1" rowspan="1" valign="top">
Thermal conductivity 
(W/mK)</th>
                                <th align="left" colspan="1" rowspan="1" valign="top">
Compressive strength (MPa)</th>
                                <th align="left" colspan="1" rowspan="1" valign="top">
Thickness (mm)</th>
                            </tr>
                        </thead>
                        <tbody>
                            <tr>
                                <td align="left" colspan="1" rowspan="1" valign="top">
                                    <bold>Mycelium</bold>
</td>
                                <td align="left" colspan="1" rowspan="1" valign="top">128</td>
                                <td align="left" colspan="1" rowspan="1" valign="top">0.03</td>
                                <td align="left" colspan="1" rowspan="1" valign="top">0.12</td>
                                <td align="left" colspan="1" rowspan="1" valign="top">75</td>
                            </tr>
                        </tbody>
                    </table>
                </table-wrap>
                <table-wrap id="T2" orientation="portrait" position="float">
                    <label>
Table 2. </label>
                    <caption>
                        <title>Insulation properties of XPS and Rockwool.
                            <sup>
                                <xref ref-type="bibr" rid="ref15">15</xref>,
                                <xref ref-type="bibr" rid="ref16">16</xref>
                            </sup>
                        </title>
                    </caption>
                    <table content-type="article-table" frame="hsides">
                        <thead>
                            <tr>
                                <th align="left" colspan="1" rowspan="1" valign="top">
Insulation material</th>
                                <th align="left" colspan="1" rowspan="1" valign="top">
Density (kg/m
                                    <sup>3</sup>)</th>
                                <th align="left" colspan="1" rowspan="1" valign="top">
Thermal conductivity 
(W/mK)</th>
                                <th align="left" colspan="1" rowspan="1" valign="top">
Compressive strength (MPa)</th>
                                <th align="left" colspan="1" rowspan="1" valign="top">
Thickness (mm)</th>
                            </tr>
                        </thead>
                        <tbody>
                            <tr>
                                <td align="left" colspan="1" rowspan="1" valign="top">
                                    <bold>XPS</bold>
</td>
                                <td align="left" colspan="1" rowspan="1" valign="top">28-40</td>
                                <td align="left" colspan="1" rowspan="1" valign="top">0.028-0.031</td>
                                <td align="left" colspan="1" rowspan="1" valign="top">0.25-0.45</td>
                                <td align="left" colspan="1" rowspan="1" valign="top">70</td>
                            </tr>
                            <tr>
                                <td align="left" colspan="1" rowspan="1" valign="top">
                                    <bold>Rockwool</bold>
</td>
                                <td align="left" colspan="1" rowspan="1" valign="top">40-120</td>
                                <td align="left" colspan="1" rowspan="1" valign="top">0.035-1.36</td>
                                <td align="left" colspan="1" rowspan="1" valign="top">0.14</td>
                                <td align="left" colspan="1" rowspan="1" valign="top">50</td>
                            </tr>
                        </tbody>
                    </table>
                </table-wrap>
            </sec>
            <sec id="sec14">
                <title>Selection criteria of the traditional insulation materials</title>
                <p>Eight factors were taken into consideration while choosing materials from the Egyptian market: fire resistance, simplicity of application, cost, absorption, pressure force, durability, water vapor transmission, and thermal conductivity.
                    <sup>
                        <xref ref-type="bibr" rid="ref13">13</xref>
                    </sup>
                </p>
                <p>To determine how mycelium would function, the simulation compares its thermal performance with two conventional materials that are utilized in Egypt.
                    <list list-type="bullet">
                        <list-item>
                            <label>&#x2022;</label>
                            <p>XPS (extruded polystyrene) insulation material is manufactured by using an extrusion process, where expansion gases and retardant are added to melted polystyrene. It has a flexible size and shape according to the use; Due to its non-permeable structure, it has low water absorption. However, its production can be harmful to the environment as it relies on Hydrofluorocarbon (HFC) and CO
                                <sub>2</sub> gases.
                                <sup>
                                    <xref ref-type="bibr" rid="ref14">14</xref>
                                </sup>
                            </p>
                        </list-item>
                        <list-item>
                            <label>&#x2022;</label>
                            <p>Rockwool insulation material is made by heating natural stones like dolomite, basalt, and diabase to extremely high temperatures (1400-1600&#x00b0;C) and transformed into fibers. These fibers are then bound together using binders like starch, oil, or resins. It can be formed into panels, rolls, mats, pipes, or molded forms in a range of thicknesses and sizes, depending on its intended application. It is frequently utilized in fire safety because of its resilience to heat and moisture. It is an excellent option for building safety because it is non-flammable.
                                <sup>
                                    <xref ref-type="bibr" rid="ref14">14</xref>
                                </sup>
                            </p>
                        </list-item>
                    </list>
                </p>
                <p>An Egyptian company specializing in building insulation materials, Rockal Al Alamia (
                    <ext-link ext-link-type="uri" xlink:href="https://www.rockalinsulation.com/">https://www.rockalinsulation.com/</ext-link>), for Insulation, provided the data shown in 
                    <xref ref-type="table" rid="T2">Table 2</xref>. Since the simulation and the materials are based in Egypt, the selection of this data source is consistent with the study&#x2019;s setting and provides relevance to regional environmental conditions and construction methods.</p>
            </sec>
        </sec>
        <sec id="sec15">
            <title>Case study</title>
            <p>Egypt&#x2019;s residential buildings heavily rely on air conditioning, increasing energy costs.
                <sup>
                    <xref ref-type="bibr" rid="ref1">1</xref>
                </sup> This research compares mycelium insulation with two commonly used materials in Egypt, such as XPS and Rockwool, on a typical floor of a Type A building in Janna Compound to assess its potential as a sustainable alternative in reducing energy consumption and enhancing thermal efficiency.</p>
            <sec id="sec16">
                <title>Thermal comfort and bioclimatic chart analysis in New Cairo, Egypt</title>
                <p>This research examines the bioclimatic conditions in Egypt utilizing Climate Consultant v.6.0 to create a psychrometric chart based on weather data. This chart aids in identifying effective design strategies aimed at improving indoor thermal comfort, as illustrated in 
                    <xref ref-type="fig" rid="f4">
Figure 4</xref>, which showcases climate-responsive design methods.</p>
                <fig fig-type="figure" id="f4" orientation="portrait" position="float">
                    <label>
Figure 4. </label>
                    <caption>
                        <title>Bioclimatic Analysis of Egypt's climate using Climate Consultant, indicating 62% comfortable hours (Red) with passive.</title>
                    </caption>
                    <graphic id="gr4" orientation="portrait" position="float" xlink:href="https://f1000research-files.f1000.com/manuscripts/179279/ba434910-9183-4f26-a6de-859c894fa5ae_figure4.gif"/>
                </fig>
                <p>
                    <xref ref-type="fig" rid="f4">
Figure 4</xref> reveals that only 18.5% annually falls within Egypt&#x2019;s thermal comfort range. Several climate-responsive design strategies were evaluated to improve thermal comfort:
                    <list list-type="bullet">
                        <list-item>
                            <label>&#x2022;</label>
                            <p>Internal heat gains could increase comfortable hours by 32.6%, particularly in winter.</p>
                        </list-item>
                        <list-item>
                            <label>&#x2022;</label>
                            <p>4.9% of thermal comfort was attributed to natural ventilation and cooling.</p>
                        </list-item>
                        <list-item>
                            <label>&#x2022;</label>
                            <p>Night ventilation, when paired with high thermal mass, enhanced comfort by 7.3%.</p>
                        </list-item>
                        <list-item>
                            <label>&#x2022;</label>
                            <p>The integration of high thermal mass and passive solar heating boosts comfort by 14.7%, resulting in 62% of the time feeling comfortable.</p>
                        </list-item>
                    </list>
                </p>
                <p>Nevertheless, the results are based on general climate data recommendations. To identify the most effective solutions, further investigation of the building&#x2019;s design parameters will be required.</p>
            </sec>
            <sec id="sec17">
                <title>Overview of New Cairo, Egypt&#x2019;s Climate</title>
                <p>New Cairo, which is located between latitudes 30.03&#x00b0;N and longitudes 31.47&#x00b0;E, has a mainly hot desert climate. Climatic data indicates that summer temperatures typically reach between 35&#x2013;45&#x00b0;C in August, highlighting the need for effective cooling systems during the hot months. In contrast, winter temperatures are lower, ranging from 15&#x2013;20&#x00b0;C, which may reduce heating requirements.
                    <sup>
                        <xref ref-type="bibr" rid="ref17">17</xref>
                    </sup>
                </p>
            </sec>
            <sec id="sec18">
                <title>Case study description of Janna Compound, New Cairo</title>
                <p>The examined building model is a type A building, part of a residential project created by the Egyptian Government to accommodate the growing need for homes. It&#x2019;s a six-story building, each containing four residential apartments, each around 130 square meters. Each flat typically houses at least two residents, and the simulation was conducted on a typical floor of the building
                    <sup>
                        <xref ref-type="bibr" rid="ref18">18</xref>
                    </sup> as shown in 
                    <xref ref-type="fig" rid="f5">
Figure 5</xref>.</p>
                <fig fig-type="figure" id="f5" orientation="portrait" position="float">
                    <label>
Figure 5. </label>
                    <caption>
                        <title>Type A building plan, typical floor of Janna Compound, New Cairo, Egypt.</title>
                    </caption>
                    <graphic id="gr5" orientation="portrait" position="float" xlink:href="https://f1000research-files.f1000.com/manuscripts/179279/ba434910-9183-4f26-a6de-859c894fa5ae_figure5.gif"/>
                </fig>
                <fig fig-type="figure" id="f6" orientation="portrait" position="float">
                    <label>
Figure 6. </label>
                    <caption>
                        <title>Sun path Diagram of the building, Janna compound.</title>
                    </caption>
                    <graphic id="gr6" orientation="portrait" position="float" xlink:href="https://f1000research-files.f1000.com/manuscripts/179279/ba434910-9183-4f26-a6de-859c894fa5ae_figure6.gif"/>
                </fig>
                <p>The typical floor plan has four similar apartments, each covering 130 sqm. Each apartment includes a kitchen, living room, two bathrooms, and three bedrooms
                    <sup>
                        <xref ref-type="bibr" rid="ref18">18</xref>
                    </sup> as shown in 
                    <xref ref-type="fig" rid="f5">
Figure 5</xref>.</p>
            </sec>
            <sec id="sec19">
                <title>Design builder project file configuration for Janna Compound case study</title>
                <p>Janna Compound is a residential gated compound developed by the Egyptian Government to accommodate the growing need for homes across multiple cities across Egypt, including El-Sheikh Zayed, Al-Minya, New Cairo, and Mansura. The project consistently utilizes the same design models (Types A and B), regardless of local climate variations or material costs.
                    <sup>
                        <xref ref-type="bibr" rid="ref19">19</xref>
                    </sup> The Type A building in New Cairo, Egypt, was selected for simulation to compare Mycelium insulation with traditional materials, as many residential projects in the area lack appropriate insulation, making this comparison crucial for enhancing energy efficiency and thermal comfort. As shown in 
                    <xref ref-type="fig" rid="f6">
Figure 6</xref>, solar exposure on the selected building provided by the sun path diagram is a key factor in thermal performance assesment.</p>
            </sec>
            <sec id="sec20">
                <title>Data entry</title>
                <p>Design Builder 7.0.2.006 with an Energy Plus 9.4 plugin is the energy simulation software used in this case study. It works by modeling the building&#x2019;s environmental conditions on an annual and monthly basis, including humidity, lighting, thermal balance, and energy consumption.</p>
                <p>

                    <italic toggle="yes">Activity</italic>
                </p>
                <p>The activity of each space is specified accurately as well as the number of users, occupancy density, and metabolic rate as shown in 
                    <xref ref-type="table" rid="T3">
Table 3</xref>.</p>
                <table-wrap id="T3" orientation="portrait" position="float">
                    <label>
Table 3. </label>
                    <caption>
                        <title>Specifications for Janna Compound activity in design builder [By the researchers].</title>
                    </caption>
                    <table content-type="article-table" frame="hsides">
                        <thead>
                            <tr>
                                <th align="left" colspan="1" rowspan="1" valign="top">Activity</th>
                                <th align="left" colspan="1" rowspan="1" valign="top"/>
                            </tr>
                        </thead>
                        <tbody>
                            <tr>
                                <td align="left" colspan="1" rowspan="1" valign="top">Template</td>
                                <td align="left" colspan="1" rowspan="1" valign="top">

                                    <italic toggle="yes">Domestic Circulation &#x2013; Residential Spaces</italic>
</td>
                            </tr>
                            <tr>
                                <td align="left" colspan="1" rowspan="1" valign="top">Number of Users</td>
                                <td align="left" colspan="1" rowspan="1" valign="top">
                                    <italic toggle="yes">2 users minimum</italic>
</td>
                            </tr>
                            <tr>
                                <td align="left" colspan="1" rowspan="1" valign="top">Occupancy Density (people/m
                                    <sup>2</sup>)</td>
                                <td align="left" colspan="1" rowspan="1" valign="top">
                                    <italic toggle="yes">0.117 people/m
                                        <sup>2</sup>
                                    </italic>
</td>
                            </tr>
                            <tr>
                                <td align="left" colspan="1" rowspan="1" valign="top">Metabolic Rate</td>
                                <td align="left" colspan="1" rowspan="1" valign="top">
                                    <italic toggle="yes">0.925 (Assuming 2 adults, 1 man and 1 woman)</italic>
</td>
                            </tr>
                        </tbody>
                    </table>
                </table-wrap>
                <p>

                    <italic toggle="yes">Construction</italic>
                </p>
                <p>Accurate simulation requires detailed building information, encompassing the materials used for external walls, slabs, and roofs. The U-value and insulation properties play a critical role in energy consumption, as they affect thermal exchange. 
                    <xref ref-type="table" rid="T4">
Table 4</xref> outlines the layers of building envelope materials utilized in the Design-Builder program.</p>
                <table-wrap id="T4" orientation="portrait" position="float">
                    <label>
Table 4. </label>
                    <caption>
                        <title>Construction layers of the base case in design builder [By the researchers].</title>
                    </caption>
                    <table content-type="article-table" frame="hsides">
                        <thead>
                            <tr>
                                <th align="left" colspan="1" rowspan="1" valign="top">

                                    <italic toggle="yes">Construction</italic>
</th>
                                <th align="left" colspan="2" rowspan="1" valign="top">

                                    <italic toggle="yes">Layers of the building envelope (Thickness, m)</italic>
</th>
                            </tr>
                        </thead>
                        <tbody>
                            <tr>
                                <td align="left" colspan="1" rowspan="1" valign="top">External wall</td>
                                <td align="left" colspan="1" rowspan="1" valign="top">The Outermost Layer (External Plaster) 0.005
                                    <break/>Cement/plaster/mortar-plaster 0.02
                                    <break/>Brickwork Outer 0.25
                                    <break/>Cement/plaster/mortar-plaster 0.02
                                    <break/>Innermost Layer (Acrylic Paint) 0.001</td>
                                <td align="left" colspan="1" rowspan="1" valign="top">

                                    <graphic id="gr16" orientation="portrait" position="float" xlink:href="https://f1000research-files.f1000.com/manuscripts/179279/ba434910-9183-4f26-a6de-859c894fa5ae_graphics1.gif"/>
</td>
                            </tr>
                            <tr>
                                <td align="left" colspan="1" rowspan="1" valign="top">Internal partition</td>
                                <td align="left" colspan="1" rowspan="1" valign="top">Gypsum Plaster 0.0012
                                    <break/>Brickwork inner 0.12
                                    <break/>Gypsum Plaster 0.0012</td>
                                <td align="left" colspan="1" rowspan="1" valign="top">
                                    <graphic id="gr17" orientation="portrait" position="float" xlink:href="https://f1000research-files.f1000.com/manuscripts/179279/ba434910-9183-4f26-a6de-859c894fa5ae_graphics2.gif"/>
</td>
                            </tr>
                            <tr>
                                <td align="left" colspan="1" rowspan="1" valign="top">Roof</td>
                                <td align="left" colspan="1" rowspan="1" valign="top">Cement/plaster/mortar-plaster 0.02
                                    <break/>Concrete 0.07
                                    <break/>EPS thermal insulation layer 0.05
                                    <break/>Reinforced concrete 2% steel 0.15
                                    <break/>Cement/plaster/mortar-plaster 0.02</td>
                                <td align="left" colspan="1" rowspan="1" valign="top">
                                    <graphic id="gr18" orientation="portrait" position="float" xlink:href="https://f1000research-files.f1000.com/manuscripts/179279/ba434910-9183-4f26-a6de-859c894fa5ae_graphics3.gif"/>
</td>
                            </tr>
                            <tr>
                                <td align="left" colspan="1" rowspan="1" valign="top">Floor</td>
                                <td align="left" colspan="1" rowspan="1" valign="top">Ceramic tiles 0.02
                                    <break/>Cement layer 0.02
                                    <break/>Sand 0.07
                                    <break/>Reinforced concrete 2% steel 0.15
                                    <break/>Cement/plaster/mortar-plaster 0.02</td>
                                <td align="left" colspan="1" rowspan="1" valign="top">
                                    <graphic id="gr19" orientation="portrait" position="float" xlink:href="https://f1000research-files.f1000.com/manuscripts/179279/ba434910-9183-4f26-a6de-859c894fa5ae_graphics4.gif"/>
</td>
                            </tr>
                        </tbody>
                    </table>
                </table-wrap>
                <p>

                    <italic toggle="yes">Openings</italic>
                </p>
                <p>Energy consumption is greatly affected by thermal gain and loss via windows, particularly in hot regions where solar thermal energy plays a significant role. For accurate simulation, detailed information on window glazing and shading systems is essential, as shown in 
                    <xref ref-type="table" rid="T5">
Table 5</xref>.</p>
                <table-wrap id="T5" orientation="portrait" position="float">
                    <label>
Table 5. </label>
                    <caption>
                        <title>Selected opening materials in design builder [By the researchers].</title>
                    </caption>
                    <table content-type="article-table" frame="hsides">
                        <thead>
                            <tr>
                                <th align="left" colspan="1" rowspan="1" valign="top">Openings</th>
                                <th align="left" colspan="1" rowspan="1" valign="top"/>
                            </tr>
                        </thead>
                        <tbody>
                            <tr>
                                <td align="left" colspan="1" rowspan="1" valign="top">Internal Door</td>
                                <td align="left" colspan="1" rowspan="1" valign="top">

                                    <italic toggle="yes">Plywood Lightweight, 0.05m thickness, 0.9 W, 2.2 H</italic>
</td>
                            </tr>
                            <tr>
                                <td align="left" colspan="1" rowspan="1" valign="top">Windows</td>
                                <td align="left" colspan="1" rowspan="1" valign="top">
                                    <italic toggle="yes">Single glazing, clear, no shading on windows, Aluminum frame</italic>
</td>
                            </tr>
                        </tbody>
                    </table>
                </table-wrap>
                <p>

                    <italic toggle="yes">HVAC</italic>
                </p>
                <p>HVAC was employed to calculate total energy consumption during the hot months to assess how mycelium insulation would reduce energy usage. However, for the calculation of PMV, PPD, and discomfort hours, the HVAC was turned off. The HVAC configurations utilized in the program are presented in 
                    <xref ref-type="table" rid="T6">
Table 6</xref> below.</p>
                <table-wrap id="T6" orientation="portrait" position="float">
                    <label>
Table 6. </label>
                    <caption>
                        <title>HVAC settings designated in design builder [By the researchers].</title>
                    </caption>
                    <table content-type="article-table" frame="hsides">
                        <thead>
                            <tr>
                                <th align="left" colspan="1" rowspan="1" valign="top">HVAC</th>
                                <th align="left" colspan="1" rowspan="1" valign="top"/>
                            </tr>
                        </thead>
                        <tbody>
                            <tr>
                                <td align="left" colspan="1" rowspan="1" valign="top">HVAC Template</td>
                                <td align="left" colspan="1" rowspan="1" valign="top">

                                    <italic toggle="yes">Split unit + Natural Ventilation</italic>
</td>
                            </tr>
                            <tr>
                                <td align="left" colspan="1" rowspan="1" valign="top">Cooling System COP</td>
                                <td align="left" colspan="1" rowspan="1" valign="top">
                                    <italic toggle="yes">1.80</italic>
</td>
                            </tr>
                            <tr>
                                <td align="left" colspan="1" rowspan="1" valign="top">Heating System COP</td>
                                <td align="left" colspan="1" rowspan="1" valign="top">
                                    <italic toggle="yes">0.85</italic>
</td>
                            </tr>
                            <tr>
                                <td align="left" colspan="1" rowspan="1" valign="top">Natural Ventilation</td>
                                <td align="left" colspan="1" rowspan="1" valign="top">
                                    <italic toggle="yes">Checked on</italic>
</td>
                            </tr>
                        </tbody>
                    </table>
                </table-wrap>
            </sec>
            <sec id="sec21">
                <title>Case study simulation findings</title>
                <p>The simulation was conducted from the beginning of January to the end of December, evaluating four different cases of different insulation materials, as summarized in 
                    <xref ref-type="table" rid="T7">
Table 7</xref>. However, when calculating PMV, PPD, and energy consumption, the simulation focused primarily on the hot months, from June 1 to August 31.
                    <list list-type="bullet">
                        <list-item>
                            <label>&#x2022;</label>
                            <p>Base Case: Brick wall without any insulating layers.</p>
                        </list-item>
                        <list-item>
                            <label>&#x2022;</label>
                            <p>Alternative-1: Mycelium Insulation</p>
                        </list-item>
                        <list-item>
                            <label>&#x2022;</label>
                            <p>Alternative-2: XPS-Extruded polystyrene Insulation</p>
                        </list-item>
                        <list-item>
                            <label>&#x2022;</label>
                            <p>Alternative-3: Rock wool Insulation
</p>
                        </list-item>
                    </list>
                </p>
                <table-wrap id="T7" orientation="portrait" position="float">
                    <label>
Table 7. </label>
                    <caption>
                        <title>Simulation four scenarios to be tested in design builder [By the researchers].</title>
                    </caption>
                    <table content-type="article-table" frame="hsides">
                        <thead>
                            <tr>
                                <th align="left" colspan="1" rowspan="1" valign="top">Case</th>
                                <th align="left" colspan="2" rowspan="1" valign="top">Insulation type</th>
                            </tr>
                        </thead>
                        <tbody>
                            <tr>
                                <td align="left" colspan="1" rowspan="2" valign="top">Base Case</td>
                                <td align="left" colspan="1" rowspan="1" valign="top">No Insulation</td>
                                <td align="left" colspan="1" rowspan="2" valign="top">

                                    <graphic id="gr20" orientation="portrait" position="float" xlink:href="https://f1000research-files.f1000.com/manuscripts/179279/ba434910-9183-4f26-a6de-859c894fa5ae_graphics5.gif"/>
</td>
                            </tr>
                            <tr>
                                <td align="left" colspan="1" rowspan="1" valign="top">

                                    <italic toggle="yes">Outermost layer: External Plaster 0.005m</italic>

                                    <break/>

                                    <italic toggle="yes">Cement/plaster/mortar-plaster 0.020m</italic>

                                    <break/>

                                    <italic toggle="yes">Brickwork Outer 0.250m</italic>

                                    <break/>

                                    <italic toggle="yes">Cement/plaster/mortar-plaster 0.020m</italic>

                                    <break/>

                                    <italic toggle="yes">Innermost layer: Acrylic 0.001m</italic>
</td>
                            </tr>
                            <tr>
                                <td align="left" colspan="1" rowspan="2" valign="top">Alternative-1</td>
                                <td align="left" colspan="1" rowspan="1" valign="top">Mycelium</td>
                                <td align="left" colspan="1" rowspan="2" valign="top">
                                    <graphic id="gr21" orientation="portrait" position="float" xlink:href="https://f1000research-files.f1000.com/manuscripts/179279/ba434910-9183-4f26-a6de-859c894fa5ae_graphics6.gif"/>
</td>
                            </tr>
                            <tr>
                                <td align="left" colspan="1" rowspan="1" valign="top">

                                    <italic toggle="yes">Outermost layer: External Plaster 0.005m</italic>

                                    <break/>

                                    <italic toggle="yes">Cement/plaster/mortar-plaster 0.020m</italic>

                                    <break/>

                                    <italic toggle="yes">Brickwork Outer 0.120m</italic>

                                    <break/>

                                    <italic toggle="yes">Mycelium Insulation panel 0.075m</italic>

                                    <break/>

                                    <italic toggle="yes">Brickwork Inner 0.120m</italic>

                                    <break/>

                                    <italic toggle="yes">Cement/plaster/mortar-plaster 0.020m</italic>

                                    <break/>

                                    <italic toggle="yes">Innermost layer: Acrylic 0.001m</italic>
</td>
                            </tr>
                            <tr>
                                <td align="left" colspan="1" rowspan="2" valign="top">Alternative-2</td>
                                <td align="left" colspan="1" rowspan="1" valign="top">XPS-Extruded polystyrene</td>
                                <td align="left" colspan="1" rowspan="2" valign="top">
                                    <graphic id="gr22" orientation="portrait" position="float" xlink:href="https://f1000research-files.f1000.com/manuscripts/179279/ba434910-9183-4f26-a6de-859c894fa5ae_graphics7.gif"/>
</td>
                            </tr>
                            <tr>
                                <td align="left" colspan="1" rowspan="1" valign="top">

                                    <italic toggle="yes">Outermost layer: External Plaster 0.005m</italic>

                                    <break/>

                                    <italic toggle="yes">Cement/plaster/mortar-plaster 0.020m</italic>

                                    <break/>

                                    <italic toggle="yes">Brickwork Outer 0.120m</italic>

                                    <break/>

                                    <italic toggle="yes">XPS Insulation panel 0.070m</italic>

                                    <break/>

                                    <italic toggle="yes">Brickwork Inner 0.120m</italic>

                                    <break/>

                                    <italic toggle="yes">Cement/plaster/mortar-plaster 0.020m Innermost layer: Acrylic 0.001m</italic>
</td>
                            </tr>
                            <tr>
                                <td align="left" colspan="1" rowspan="2" valign="top">Alternative-3</td>
                                <td align="left" colspan="1" rowspan="1" valign="top">Rockwool</td>
                                <td align="left" colspan="1" rowspan="2" valign="top">
                                    <graphic id="gr23" orientation="portrait" position="float" xlink:href="https://f1000research-files.f1000.com/manuscripts/179279/ba434910-9183-4f26-a6de-859c894fa5ae_graphics8.gif"/>
</td>
                            </tr>
                            <tr>
                                <td align="left" colspan="1" rowspan="1" valign="top">

                                    <italic toggle="yes">Outermost layer: External Plaster 0.005m</italic>

                                    <break/>

                                    <italic toggle="yes">Rockwool 0.050m</italic>

                                    <break/>

                                    <italic toggle="yes">Cement/plaster/mortar-plaster 0.020m</italic>

                                    <break/>

                                    <italic toggle="yes">Brickwork Outer 0.250m</italic>

                                    <break/>

                                    <italic toggle="yes">Cement/plaster/mortar-plaster 0.020m</italic>

                                    <break/>

                                    <italic toggle="yes">Innermost layer: Acrylic 0.001m</italic>
</td>
                            </tr>
                        </tbody>
                    </table>
                </table-wrap>
                <p>Energy-efficient building design focuses on managing exterior thermophysical properties, such as the U-value (thermal transmittance), which is key for assessing thermal efficiency and energy savings. 
                    <xref ref-type="fig" rid="f7">
Figure 7</xref> shows that the base case without insulation has the highest U-value at 1.71 W/m
                    <sup>2</sup>K, while Mycelium insulation has the lowest at 0.323 W/m
                    <sup>2</sup>K. XPS and Rock Wool have U-values of 0.34 W/m
                    <sup>2</sup>K and 0.61 W/m
                    <sup>2</sup>K, respectively, indicating varying levels of thermal performance. Therefore, Mycelium demonstrates the best U-value compared to other traditional materials.</p>
                <fig fig-type="figure" id="f7" orientation="portrait" position="float">
                    <label>
Figure 7. </label>
                    <caption>
                        <title>Thermal transmittance (U-Value) for the Four Scenarios.</title>
                    </caption>
                    <graphic id="gr7" orientation="portrait" position="float" xlink:href="https://f1000research-files.f1000.com/manuscripts/179279/ba434910-9183-4f26-a6de-859c894fa5ae_figure7.gif"/>
                </fig>
                <p>The simulation, based on ASHRAE 55&#x2013;2004 standards, evaluates discomfort hours due to inadequate cooling, heating, and humidity levels without HVAC systems. As shown in 
                    <xref ref-type="fig" rid="f8">
Figure 8</xref>, the base case with a conventional wall recorded 1986 discomfort hours annually. Mycelium insulation reduced this to 1481.71 hours, while XPS reduced it to 1485.04 hours, and Rock Wool to 1616.88 hours. The results show that Mycelium performs similarly to XPS, indicating that it can compete with traditional materials in terms of thermal comfort.</p>
                <fig fig-type="figure" id="f8" orientation="portrait" position="float">
                    <label>
Figure 8. </label>
                    <caption>
                        <title>Discomfort hours/year for the four different scenarios.</title>
                    </caption>
                    <graphic id="gr8" orientation="portrait" position="float" xlink:href="https://f1000research-files.f1000.com/manuscripts/179279/ba434910-9183-4f26-a6de-859c894fa5ae_figure8.gif"/>
                </fig>
                <p>The percentage of discomfort hours per year for the four scenarios is as follows: the base case recorded 22.6% discomfort, while Mycelium showed 16.9%, XPS recorded 16.95%, and Rock Wool had 18.4% discomfort. These results demonstrate that Mycelium not only performs better than Rock Wool but also shows a similar percentage of discomfort hours to XPS, making Mycelium an equally effective choice for improving thermal comfort and energy efficiency. As shown in 
                    <xref ref-type="fig" rid="f9">
Figure 9</xref>.</p>
                <fig fig-type="figure" id="f9" orientation="portrait" position="float">
                    <label>
Figure 9. </label>
                    <caption>
                        <title>Percentage of Discomfort hours/year for the four different scenarios.</title>
                    </caption>
                    <graphic id="gr9" orientation="portrait" position="float" xlink:href="https://f1000research-files.f1000.com/manuscripts/179279/ba434910-9183-4f26-a6de-859c894fa5ae_figure9.gif"/>
                </fig>
                <p>From June 1 to September 30, specific thermal comfort strategies are applied to enhance comfort during the summer and minimize air conditioning use. The evaluation focuses on Predicted Mean Vote (PMV) and Percentage of People Dissatisfied (PPD%), using a PMV scale from -3 (cool) to +3 (hot). By concentrating on the hot months, as shown in 
                    <xref ref-type="fig" rid="f10">
Figure 10</xref>, this approach allows for precise adjustments to environmental systems, ensuring optimal thermal comfort and energy efficiency during peak cooling periods.</p>
                <fig fig-type="figure" id="f10" orientation="portrait" position="float">
                    <label>
Figure 10. </label>
                    <caption>
                        <title>PMV (Predicted Mean Vote) Scaling Factors.</title>
                    </caption>
                    <graphic id="gr10" orientation="portrait" position="float" xlink:href="https://f1000research-files.f1000.com/manuscripts/179279/ba434910-9183-4f26-a6de-859c894fa5ae_figure10.gif"/>
                </fig>
                <p>
                    <xref ref-type="table" rid="T8">
Table 8</xref> illustrates the variations in the outcomes relative to the baseline case across all four scenarios, emphasizing the PPD% values during the warmest months.</p>
                <table-wrap id="T8" orientation="portrait" position="float">
                    <label>
Table 8. </label>
                    <caption>
                        <title>PMV-PPD% in hot months for the four scenarios in design builder [By the researchers].</title>
                    </caption>
                    <table content-type="article-table" frame="hsides">
                        <thead>
                            <tr>
                                <th align="left" colspan="1" rowspan="1" valign="top">Scenarios</th>
                                <th align="left" colspan="1" rowspan="1" valign="top">Max Fanger PMV 
(Hot months)</th>
                                <th align="left" colspan="1" rowspan="1" valign="top">
Max Fanger PPD% 
(Hot months)</th>
                            </tr>
                        </thead>
                        <tbody>
                            <tr>
                                <td align="left" colspan="1" rowspan="1" valign="top">Base Case</td>
                                <td align="left" colspan="1" rowspan="1" valign="top">
                                    <italic toggle="yes">1.46</italic>
</td>
                                <td align="left" colspan="1" rowspan="1" valign="top">
                                    <italic toggle="yes">53.26%</italic>
</td>
                            </tr>
                            <tr>
                                <td align="left" colspan="1" rowspan="1" valign="top">Alternative-1</td>
                                <td align="left" colspan="1" rowspan="1" valign="top">
                                    <italic toggle="yes">1.37</italic>
</td>
                                <td align="left" colspan="1" rowspan="1" valign="top">
                                    <italic toggle="yes">50.48%</italic>
</td>
                            </tr>
                            <tr>
                                <td align="left" colspan="1" rowspan="1" valign="top">Alternative-2</td>
                                <td align="left" colspan="1" rowspan="1" valign="top">
                                    <italic toggle="yes">1.37</italic>
</td>
                                <td align="left" colspan="1" rowspan="1" valign="top">
                                    <italic toggle="yes">50.46%</italic>
</td>
                            </tr>
                            <tr>
                                <td align="left" colspan="1" rowspan="1" valign="top">Alternative-3</td>
                                <td align="left" colspan="1" rowspan="1" valign="top">
                                    <italic toggle="yes">1.35</italic>
</td>
                                <td align="left" colspan="1" rowspan="1" valign="top">
                                    <italic toggle="yes">49.76%</italic>
</td>
                            </tr>
                        </tbody>
                    </table>
                </table-wrap>
                <table-wrap id="T9" orientation="portrait" position="float">
                    <label>
Table 9. </label>
                    <caption>
                        <title>Comparison between all scenarios results [By the researchers].</title>
                    </caption>
                    <table content-type="article-table" frame="hsides">
                        <thead>
                            <tr>
                                <th align="left" colspan="5" rowspan="1" valign="top">Comparison between the four scenarios</th>
                            </tr>
                            <tr>
                                <th align="left" colspan="1" rowspan="1" valign="top">
Scenario</th>
                                <th align="left" colspan="1" rowspan="1" valign="top">
Base case 

                                    <graphic id="gr24" orientation="portrait" position="float" xlink:href="https://f1000research-files.f1000.com/manuscripts/179279/ba434910-9183-4f26-a6de-859c894fa5ae_graphics9.gif"/>
</th>
                                <th align="left" colspan="1" rowspan="1" valign="top">
Mycelium 

                                    <graphic id="gr25" orientation="portrait" position="float" xlink:href="https://f1000research-files.f1000.com/manuscripts/179279/ba434910-9183-4f26-a6de-859c894fa5ae_graphics10.gif"/>
</th>
                                <th align="left" colspan="1" rowspan="1" valign="top">
XPS 

                                    <graphic id="gr26" orientation="portrait" position="float" xlink:href="https://f1000research-files.f1000.com/manuscripts/179279/ba434910-9183-4f26-a6de-859c894fa5ae_graphics11.gif"/>
</th>
                                <th align="left" colspan="1" rowspan="1" valign="top">
Rock wool 

                                    <graphic id="gr27" orientation="portrait" position="float" xlink:href="https://f1000research-files.f1000.com/manuscripts/179279/ba434910-9183-4f26-a6de-859c894fa5ae_graphics12.gif"/>
</th>
                            </tr>
                        </thead>
                        <tbody>
                            <tr>
                                <td align="left" colspan="1" rowspan="1" valign="top">U-Value
</td>
                                <td align="left" colspan="1" rowspan="1" valign="top">1.71</td>
                                <td align="left" colspan="1" rowspan="1" valign="top">0.323</td>
                                <td align="left" colspan="1" rowspan="1" valign="top">0.34</td>
                                <td align="left" colspan="1" rowspan="1" valign="top">0.61</td>
                            </tr>
                            <tr>
                                <td align="left" colspan="1" rowspan="1" valign="top">% of Discomfort hours/year</td>
                                <td align="left" colspan="1" rowspan="1" valign="top">22.6%</td>
                                <td align="left" colspan="1" rowspan="1" valign="top">16.9</td>
                                <td align="left" colspan="1" rowspan="1" valign="top">16.9</td>
                                <td align="left" colspan="1" rowspan="1" valign="top">18.4</td>
                            </tr>
                            <tr>
                                <td align="left" colspan="1" rowspan="1" valign="top">% of Energy reduction</td>
                                <td align="left" colspan="1" rowspan="1" valign="top">-</td>
                                <td align="left" colspan="1" rowspan="1" valign="top">15.8</td>
                                <td align="left" colspan="1" rowspan="1" valign="top">15.7</td>
                                <td align="left" colspan="1" rowspan="1" valign="top">13.3</td>
                            </tr>
                            <tr>
                                <td align="left" colspan="1" rowspan="1" valign="top">PMV</td>
                                <td align="left" colspan="1" rowspan="1" valign="top">1.46</td>
                                <td align="left" colspan="1" rowspan="1" valign="top">1.37</td>
                                <td align="left" colspan="1" rowspan="1" valign="top">1.37</td>
                                <td align="left" colspan="1" rowspan="1" valign="top">1.35</td>
                            </tr>
                        </tbody>
                    </table>
                </table-wrap>
                <p>As shown in 
                    <xref ref-type="table" rid="T9">
Table 9</xref>, the comparison of the four scenarios in the case study offers key insights for improving thermal comfort. In the base case, without insulation, the Predicted Mean Vote (PMV) is 1.46, with a Percentage of People Dissatisfied (PPD) of 53.26%. Adding Mycelium insulation in Alternative-1 improves the PMV to 1.37 and reduces the PPD to 50.48%. Alternative-2, which uses XPS insulation, shows similar results, with a PMV of 1.37 and a PPD of 50.46%. Alternative-3, featuring Rock Wool insulation, slightly improves comfort further, achieving a PMV of 1.35 and a PPD of 49.76%. As shown in 
                    <xref ref-type="fig" rid="f11">
Figures 11</xref> and 
                    <xref ref-type="fig" rid="f12">12</xref>.</p>
                <fig fig-type="figure" id="f11" orientation="portrait" position="float">
                    <label>
Figure 11. </label>
                    <caption>
                        <title>PMV (Predicted Mean Vote) for the Four Simulation Scenarios in Design Builder.</title>
                    </caption>
                    <graphic id="gr11" orientation="portrait" position="float" xlink:href="https://f1000research-files.f1000.com/manuscripts/179279/ba434910-9183-4f26-a6de-859c894fa5ae_figure11.gif"/>
                </fig>
                <fig fig-type="figure" id="f12" orientation="portrait" position="float">
                    <label>
Figure 12. </label>
                    <caption>
                        <title>PPD% (Predicted Percentage of Dissatisfaction) for the Four Simulation Scenarios in Design Builder.</title>
                    </caption>
                    <graphic id="gr12" orientation="portrait" position="float" xlink:href="https://f1000research-files.f1000.com/manuscripts/179279/ba434910-9183-4f26-a6de-859c894fa5ae_figure12.gif"/>
                </fig>
                <p>Mycelium, with its impressive performance values, not only matches traditional insulation materials in terms of thermal efficiency but also provides added advantages. As a sustainable and biodegradable material, Mycelium is an eco-friendly choice, making it a highly suitable option for thermal insulation.</p>
                <p>The simulation of annual energy consumption reveals that electricity usage varies throughout the year, with a notable increase during the summer months. In contrast, consumption is relatively low during the cooler months, as heating and cooling needs are reduced. However, from May to August, energy consumption peaks due to the heightened cooling demands driven by intense solar radiation. As shown in 
                    <xref ref-type="fig" rid="f13">
Figure 13</xref>, this simulation underscores the significant effect of seasonal changes on overall energy performance.</p>
                <fig fig-type="figure" id="f13" orientation="portrait" position="float">
                    <label>
Figure 13. </label>
                    <caption>
                        <title>Energy consumption for the baseline case from design builder.</title>
                    </caption>
                    <graphic id="gr13" orientation="portrait" position="float" xlink:href="https://f1000research-files.f1000.com/manuscripts/179279/ba434910-9183-4f26-a6de-859c894fa5ae_figure13.gif"/>
                </fig>
                <p>Regarding energy consumption, it is observed that the base case wall material led to higher energy usage during the warmer months. Particularly from May to August, due to increased solar radiation. The simulation findings indicate that the total electricity consumption for the base case during these hot months was 10,203.75 kWh. In comparison, XPS insulation resulted in 8,601.1 kWh, Mycelium insulation consumed 8,587.42 kWh, and Rock Wool insulation had a consumption of 8,838.77 kWh. As shown in 
                    <xref ref-type="fig" rid="f14">
Figure 14</xref>.</p>
                <fig fig-type="figure" id="f14" orientation="portrait" position="float">
                    <label>
Figure 14. </label>
                    <caption>
                        <title>Energy consumption for the 4 scenarios in hot months in design builder.</title>
                    </caption>
                    <graphic id="gr14" orientation="portrait" position="float" xlink:href="https://f1000research-files.f1000.com/manuscripts/179279/ba434910-9183-4f26-a6de-859c894fa5ae_figure14.gif"/>
                </fig>
                <p>The energy consumption reductions are 15.7% for XPS, 15.8% for Mycelium, and 13.3% for Rock Wool. As shown in 
                    <xref ref-type="fig" rid="f15">
Figure 15</xref>. These results show that Mycelium performs similarly to XPS in terms of energy savings and makes a notable contribution to improving energy efficiency. According to these results, CO
                    <sub>2</sub> emission was also reduced, Mycelium reduced 979.5 kg of CO
                    <sub>2</sub>, while XPS reduced 971.2 kg and Rock wool reduced 827.18 kg of CO
                    <sub>2</sub>.</p>
                <fig fig-type="figure" id="f15" orientation="portrait" position="float">
                    <label>
Figure 15. </label>
                    <caption>
                        <title>Percentage of energy reduction for the 4 scenarios in hot months in design builder.</title>
                    </caption>
                    <graphic id="gr15" orientation="portrait" position="float" xlink:href="https://f1000research-files.f1000.com/manuscripts/179279/ba434910-9183-4f26-a6de-859c894fa5ae_figure15.gif"/>
                </fig>
                <p>To calculate the percentage of energy reduction, the energy consumption of the base case scenario (without intervention) is compared to the improved scenario (with intervention). The formula used is:
                    <disp-formula>

                        <mml:math display="block">
                            <mml:mtext>Percentage&#x2009;of&#x2009;Energy&#x2009;Reduction</mml:mtext>
                            <mml:mo>=</mml:mo>
                            <mml:mrow>
                                <mml:mo stretchy="true">(</mml:mo>
                                <mml:mfrac>
                                    <mml:mrow>
                                        <mml:mtext>Base&#x2009;case&#x2009;Energy&#x2009;Consumption</mml:mtext>
                                        <mml:mo>&#x2212;</mml:mo>
                                        <mml:mtext>Improved&#x2009;Energy&#x2009;Consumption</mml:mtext>
                                    </mml:mrow>
                                    <mml:mtext>Base&#x2009;case&#x2009;Energy&#x2009;Consumption</mml:mtext>
                                </mml:mfrac>
                                <mml:mo stretchy="true">)</mml:mo>
                            </mml:mrow>
                            <mml:mo>&#x00d7;</mml:mo>
                            <mml:mn>100</mml:mn>
                        </mml:math>
</disp-formula>
                </p>
            </sec>
        </sec>
        <sec id="sec22">
            <title>Conclusions and recommendations</title>
            <p>The simulation was performed on a typical floor of a Type A building in New Cairo&#x2019;s Janna compound. Each floor had four apartments, each approximately 130 m
                <sup>2</sup>. The research compared Mycelium insulation to traditional materials like XPS and Rock Wool in terms of their thermal performance and energy efficiency.</p>
            <p>The results showed that Mycelium insulation performed effectively, with a PPD of 50.8% and a PMV of 1.37. This result was much better than the base case and equivalent to XPS (PPD = 50.46%, PMV = 1.37). In terms of U-value, Mycelium demonstrated the best performance with a U-value of 0.323 W/m
                <sup>2</sup>K, compared to XPS at 0.34 W/m
                <sup>2</sup>K and Rock Wool at 0.61 W/m
                <sup>2</sup>K. Regarding energy consumption, Mycelium reduced energy usage by 15.8%, which was better than Rock wool (13.3%), and comparable to XPS (15.7%). These results highlight Mycelium as a sustainable and energy-efficient alternative to traditional insulation materials.</p>
            <p>In conclusion, Mycelium and XPS have nearly the same values. However, XPS (Extruded polystyrene) is well-known and widely used because of its affordability, availability, and strong thermal performance. Because XPS lacks a porous structure, it creates a sealed indoor environment that helps the growth of Mold and algae. It also has a negative impact on indoor air quality, low biodegradability, and fire safety (producing burning droplets and excessive smoke). It degrades building surfaces and lowers indoor air quality, which may cause health problems for building occupants.
                <sup>
                    <xref ref-type="bibr" rid="ref9">9</xref>
                </sup>
            </p>
            <p>In Contrast, Mycelium Insulation offers a sustainable and circular alternative with several key advantages:
                <list list-type="bullet">
                    <list-item>
                        <label>&#x2022;</label>
                        <p>Cost-effectiveness: Mycelium grows using agricultural waste, making it an inexpensive and available resource</p>
                    </list-item>
                    <list-item>
                        <label>&#x2022;</label>
                        <p>Biodegradability: Mycelium naturally decomposes at the end of its life cycle</p>
                    </list-item>
                    <list-item>
                        <label>&#x2022;</label>
                        <p>Rapid manufacture: it can be cultivated in a few weeks</p>
                    </list-item>
                    <list-item>
                        <label>&#x2022;</label>
                        <p>Flexibility: it can be molded into different shapes and densities</p>
                    </list-item>
                    <list-item>
                        <label>&#x2022;</label>
                        <p>Minimal Energy Consumption: Growing mycelium required less energy compared to other materials</p>
                    </list-item>
                    <list-item>
                        <label>&#x2022;</label>
                        <p>Cradle-to-cradle life cycle: Mycelium fits with the circularity idea, it can be composed and reintegrated into the natural ecosystem with no harm to the environment</p>
                    </list-item>
                </list>
            </p>
            <p>Given all these advantages, Mycelium is a promising alternative to harmful and toxic materials, aligning with the global effort to promote sustainable materials.</p>
            <p>Given the positive results of this research, it is essential to further explore how Mycelium can be integrated into the construction industry and its potential for large-scale implementation. The following recommendations aim to guide future efforts in adopting Mycelium as a mainstream insulation material.
                <list list-type="bullet">
                    <list-item>
                        <label>&#x2022;</label>
                        <p>Further Research: To examine the longevity and economic feasibility of mycelium in various climate conditions and building types.</p>
                    </list-item>
                    <list-item>
                        <label>&#x2022;</label>
                        <p>Adoption by the Construction sector: Encourage the use of Mycelium in construction, especially in regions where traditional materials contribute to environmental pollution.</p>
                    </list-item>
                    <list-item>
                        <label>&#x2022;</label>
                        <p>Regulatory Support: Advocate for policies that promote the use of sustainable, non-toxic insulation materials like Mycelium, which can significantly contribute to energy-efficient buildings and the reduction of carbon footprints</p>
                    </list-item>
                </list>
            </p>
        </sec>
        <sec id="sec24">
            <title>Ethics and consent</title>
            <p>Ethical approval and consent were not required.</p>
        </sec>
    </body>
    <back>
        <sec id="sec27" sec-type="data-availability">
            <title>Data availability</title>
            <sec id="sec28">
                <title>Underlying data</title>
                <p>Zenodo: Evaluating Mycelium as an Insulation Material: A Comparative Study on Thermal Performance, Comfort, and Energy Efficiency. 
                    <ext-link ext-link-type="uri" xlink:href="https://doi.org/10.5281/zenodo.15020538">https://doi.org/10.5281/zenodo.15020538</ext-link>
                    <sup>
                        <xref ref-type="bibr" rid="ref20">20</xref>
                    </sup>
                </p>
                <p>This project contains the following underlying data
                    <list list-type="order">
                        <list-item>
                            <label>1.</label>
                            <p>Egypt Energy Consumption Graph (Illustrates the percentage distribution of energy consumption across various sectors)</p>
                        </list-item>
                        <list-item>
                            <label>2.</label>
                            <p>The Fungi Structure (Illustrates the structure of the mushroom, underground mycelium, and the fruit body created using AI tools and modified in photoshop)</p>
                        </list-item>
                        <list-item>
                            <label>3.</label>
                            <p>The sustainable life cycle of materials sourced from mycelium (shows the sustainable life cycle of mycelium products)</p>
                        </list-item>
                        <list-item>
                            <label>4.</label>
                            <p>Bioclimatic Analysis of Egypt&#x2019;s climate Using Climate Consultant (shows climatic data such as temperature patterns, humidity levels, and solar radiation across Egypt.)</p>
                        </list-item>
                        <list-item>
                            <label>5.</label>
                            <p>Type A Building Plan, Typical Floor of Janna Compound, New Cairo, Egypt (Case study typical floor plan, designed in design builder)</p>
                        </list-item>
                        <list-item>
                            <label>6.</label>
                            <p>Sun path Diagram of the building, Janna compound (Sun path diagram in Janna compound)</p>
                        </list-item>
                        <list-item>
                            <label>7.</label>
                            <p>Thermal transmittance (U-Value) for the Four Scenarios</p>
                        </list-item>
                        <list-item>
                            <label>8.</label>
                            <p>Discomfort hours/year for the four different scenarios</p>
                        </list-item>
                        <list-item>
                            <label>9.</label>
                            <p>Percentage of Discomfort hours/year for the four different scenarios</p>
                        </list-item>
                        <list-item>
                            <label>10.</label>
                            <p>PMV (Predicted Mean Vote) Scaling Factors</p>
                        </list-item>
                        <list-item>
                            <label>11.</label>
                            <p>PMV (Predicted Mean Vote) for the Four Simulation Scenarios in Design Builder</p>
                        </list-item>
                        <list-item>
                            <label>12.</label>
                            <p>PPD% (Predicted Percentage of Dissatisfaction) for the Four Simulation Scenarios in Design Builder</p>
                        </list-item>
                        <list-item>
                            <label>13.</label>
                            <p>Energy Consumption for the Baseline Case from Design Builder</p>
                        </list-item>
                        <list-item>
                            <label>14.</label>
                            <p>Energy Consumption for the 4 Scenarios in Hot Months in Design Builder</p>
                        </list-item>
                        <list-item>
                            <label>15.</label>
                            <p>Percentage of Energy Reduction for the 4 Scenarios in Hot Months in Design Builder</p>
                        </list-item>
                        <list-item>
                            <label>16.</label>
                            <p>Excel file for Cairo Weather Data (The same weather file was used in the design builder for simulation data)</p>
                        </list-item>
                        <list-item>
                            <label>17.</label>
                            <p>Janna Compound, New Cairo (Two design builder files include the 3D model and the simulation one with HVAC and one without)</p>
                        </list-item>
                    </list>
                </p>
                <p>Data are available under the terms of the 
                    <ext-link ext-link-type="uri" xlink:href="https://creativecommons.org/licenses/by/4.0/">Creative Commons Attribution 4.0 International license</ext-link> (CC-BY 4.0).</p>
            </sec>
        </sec>
        <sec id="sec23">
            <title>Software availability statement</title>
            <p>Design-Builder v7.3.0.046 (
                <ext-link ext-link-type="uri" xlink:href="https://designbuilder.co.uk/">https://designbuilder.co.uk/</ext-link>) was used for energy simulation modeling. This is proprietary software, however, an open-access alternative such as 
                <ext-link ext-link-type="uri" xlink:href="https://openstudio.net/">OpenStudio</ext-link> can achieve similar outcomes.</p>
            <p>EnergyPlus (
                <ext-link ext-link-type="uri" xlink:href="https://energyplus.net/">https://energyplus.net/</ext-link>) is an open-access tool used in this research.</p>
        </sec>
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    </back>
    <sub-article article-type="reviewer-report" id="report392628">
        <front-stub>
            <article-id pub-id-type="doi">10.5256/f1000research.179279.r392628</article-id>
            <title-group>
                <article-title>Reviewer response for version 1</article-title>
            </title-group>
            <contrib-group>
                <contrib contrib-type="author">
                    <name>
                        <surname>Teoh</surname>
                        <given-names>Jia Heng</given-names>
                    </name>
                    <xref ref-type="aff" rid="r392628a1">1</xref>
                    <role>Referee</role>
                </contrib>
                <aff id="r392628a1">
                    <label>1</label>Nanyang Technological University (Ringgold ID: 54761), Singapore, Singapore</aff>
            </contrib-group>
            <author-notes>
                <fn fn-type="conflict">
                    <p>
                        <bold>Competing interests: </bold>No competing interests were disclosed.</p>
                </fn>
            </author-notes>
            <pub-date pub-type="epub">
                <day>9</day>
                <month>8</month>
                <year>2025</year>
            </pub-date>
            <permissions>
                <copyright-statement>Copyright: &#x00a9; 2025 Teoh JH</copyright-statement>
                <copyright-year>2025</copyright-year>
                <license xlink:href="https://creativecommons.org/licenses/by/4.0/">
                    <license-p>This is an open access peer review report distributed under the terms of the Creative Commons Attribution Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.</license-p>
                </license>
            </permissions>
            <related-article ext-link-type="doi" id="relatedArticleReport392628" related-article-type="peer-reviewed-article" xlink:href="10.12688/f1000research.162989.1"/>
            <custom-meta-group>
                <custom-meta>
                    <meta-name>recommendation</meta-name>
                    <meta-value>approve-with-reservations</meta-value>
                </custom-meta>
            </custom-meta-group>
        </front-stub>
        <body>
            <p>In the study titled, &#x201c;Evaluating mycelium as an insulation material: A comparative study on thermal performance, comfort, and energy efficiency&#x201d;, Khaled, Hany and Mossad have evaluated the use of mycelium-based materials as an insulation material in Egypt through simulations using a commercial software. Their studies have shown that compared to two other conventional insulation materials (XPS and Rockwool), mycelium-based materials performed better than or at the very least comparable to XPS and Rockwool in metrics relating to thermal performance and energy efficiency.</p>
            <p> </p>
            <p> The following are my comments regarding the study. 
                <list list-type="order">
                    <list-item>
                        <p>Please double check the definition of mycelium stated at the beginning of the literature review. The description fits the fruiting body more than mycelium, the vegetative or root-like structure of the fungus.</p>
                    </list-item>
                    <list-item>
                        <p>How easily accessible is mycelium in the area of the case study? What is the feasibility of fabricating mycelium-based material using locally available resources in Egypt? While mycelium-based materials are sustainable, if mycelium and the substrate need to be imported, the carbon footprint and energy consumed in transportation will negatively affect its sustainability.</p>
                    </list-item>
                    <list-item>
                        <p>Regarding the mycelium-based material that is being simulated in this study, what is the fungal species and the substrate or agricultural waste being assumed in this study? Literature has shown that mycelium-based material can be made with a variety of fungal species and substrates and as such, a bit more specificity is required.&#x00a0;</p>
                    </list-item>
                    <list-item>
                        <p>Please include references to justify that XPS and Rockwool are indeed traditional insulation materials commonly used in Egypt. This is unclear to readers who are not based in Egypt.&#x00a0;</p>
                    </list-item>
                </list>
            </p>
            <p>Is the case presented with sufficient detail to be useful for teaching or other practitioners?</p>
            <p>Yes</p>
            <p>Is the work clearly and accurately presented and does it cite the current literature?</p>
            <p>Yes</p>
            <p>If applicable, is the statistical analysis and its interpretation appropriate?</p>
            <p>Yes</p>
            <p>Are all the source data underlying the results available to ensure full reproducibility?</p>
            <p>Yes</p>
            <p>Are the conclusions drawn adequately supported by the results?</p>
            <p>Yes</p>
            <p>Is the background of the case&#x2019;s history and progression described in sufficient detail?</p>
            <p>Partly</p>
            <p>Reviewer Expertise:</p>
            <p>Advanced fabrication and characterization of mycelium-bound composites and fungal-based engineered living materials.</p>
            <p>I confirm that I have read this submission and believe that I have an appropriate level of expertise to confirm that it is of an acceptable scientific standard, however I have significant reservations, as outlined above.</p>
        </body>
        <sub-article article-type="response" id="comment15063-392628">
            <front-stub>
                <contrib-group>
                    <contrib contrib-type="author">
                        <name>
                            <surname>khaled</surname>
                            <given-names>passant</given-names>
                        </name>
                        <aff>Interior, Arab Academy for Science Technology and Maritime Transport College of Engineering and Technology, Alexandria, Alexandria Governorate, Egypt</aff>
                    </contrib>
                </contrib-group>
                <author-notes>
                    <fn fn-type="conflict">
                        <p>
                            <bold>Competing interests: </bold>No competing interests were disclosed.</p>
                    </fn>
                </author-notes>
                <pub-date pub-type="epub">
                    <day>12</day>
                    <month>12</month>
                    <year>2025</year>
                </pub-date>
            </front-stub>
            <body>
                <p>
                    <bold>Reviewer comment 1:</bold>
                </p>
                <p> 
                    <italic>&#x201c;Please double check the definition of mycelium stated at the beginning of the literature review. The description fits the fruiting body more than mycelium, the vegetative or root-like structure of the fungus.&#x201d;</italic>
                </p>
                <p> 
                    <bold>Author response:</bold>
                </p>
                <p> Thank you for this helpful observation. The definition of mycelium in the literature review has been corrected to accurately describe mycelium as the vegetative, filamentous network (hyphae) of a fungus rather than the fruiting body.</p>
                <p> </p>
                <p> 
                    <bold>Reviewer comment 2:</bold>
                </p>
                <p> 
                    <italic>&#x201c;How easily accessible is mycelium in the area of the case study?</italic>
                </p>
                <p> 
                    <bold>Author response:</bold>
                </p>
                <p> We appreciate this important point. A new paragraph has been added to the manuscript describing the current accessibility of mycelium in Egypt and the feasibility of local production. The text highlights emerging companies such as Cupmena and Mycelium Egypt, which cultivate mycelium using agricultural waste, demonstrating that mycelium-based materials can be produced locally without relying on imports. This addresses the sustainability concern and clarifies the material&#x2019;s local availability.</p>
            </body>
        </sub-article>
    </sub-article>
    <sub-article article-type="reviewer-report" id="report392627">
        <front-stub>
            <article-id pub-id-type="doi">10.5256/f1000research.179279.r392627</article-id>
            <title-group>
                <article-title>Reviewer response for version 1</article-title>
            </title-group>
            <contrib-group>
                <contrib contrib-type="author">
                    <name>
                        <surname>Carcassi</surname>
                        <given-names>Olga Beatrice</given-names>
                    </name>
                    <xref ref-type="aff" rid="r392627a1">1</xref>
                    <role>Referee</role>
                </contrib>
                <aff id="r392627a1">
                    <label>1</label>Columbia University, New York, New York, USA</aff>
            </contrib-group>
            <author-notes>
                <fn fn-type="conflict">
                    <p>
                        <bold>Competing interests: </bold>No competing interests were disclosed.</p>
                </fn>
            </author-notes>
            <pub-date pub-type="epub">
                <day>8</day>
                <month>8</month>
                <year>2025</year>
            </pub-date>
            <permissions>
                <copyright-statement>Copyright: &#x00a9; 2025 Carcassi OB</copyright-statement>
                <copyright-year>2025</copyright-year>
                <license xlink:href="https://creativecommons.org/licenses/by/4.0/">
                    <license-p>This is an open access peer review report distributed under the terms of the Creative Commons Attribution Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.</license-p>
                </license>
            </permissions>
            <related-article ext-link-type="doi" id="relatedArticleReport392627" related-article-type="peer-reviewed-article" xlink:href="10.12688/f1000research.162989.1"/>
            <custom-meta-group>
                <custom-meta>
                    <meta-name>recommendation</meta-name>
                    <meta-value>approve-with-reservations</meta-value>
                </custom-meta>
            </custom-meta-group>
        </front-stub>
        <body>
            <p>
                <bold>General Comments:</bold>
            </p>
            <p> The paper provides a timely and relevant comparison between conventional insulation materials (XPS, Rockwool) and a bio-based alternative&#x2014;mycelium. The use of Egyptian housing as a case study adds valuable regional relevance and could offer meaningful insights into sustainable construction practices in hot climates.</p>
            <p> However, while the research addresses an important topic and offers promising insights, the current version suffers from issues related to clarity, limited referencing, insufficient justification of methodology choices, and a lack of strong data to support its conclusions. Below are detailed comments categorized for clarity.</p>
            <p> 
                <bold>1. Clarity, Structure, and Referencing</bold> 
                <list list-type="bullet">
                    <list-item>
                        <p>
                            <bold>Clarity:</bold> The paper would benefit from substantial editing for overall coherence. Key sections (especially the background and conclusion) are unclear or contain vague statements that need further development and evidence by expanding the&#x00a0;literature review</p>
                    </list-item>
                    <list-item>
                        <p>
                            <bold>Referencing:</bold> The literature review and referencing are insufficient. 
                            <list list-type="bullet">
                                <list-item>
                                    <p>Ref [1] is overused throughout the paper. It is essential to engage with a broader range of literature.</p>
                                </list-item>
                                <list-item>
                                    <p>
                                        <bold>Recommendation:</bold> In the Introduction and Background sections, aim for at least one reference per factual statement, ideally two. This should also apply to the Conclusion section.</p>
                                </list-item>
                                <list-item>
                                    <p>Include mycelium-specific references. The field has a rapidly growing body of literature, including studies on thermal conductivity, lifecycle assessment, biodegradability, and production methods.</p>
                                </list-item>
                                <list-item>
                                    <p>Avoid generalizations without backing data. Each claim, especially those that compare performance or environmental impact, must be supported with references.</p>
                                </list-item>
                            </list> </p>
                    </list-item>
                </list> </p>
            <p> 
                <bold>2. Figures and Visuals</bold> 
                <list list-type="bullet">
                    <list-item>
                        <p>
                            <bold>Figure 1:</bold> Please add the source of the data presented in this figure. It is crucial to ensure transparency in data visualization.</p>
                    </list-item>
                    <list-item>
                        <p>
                            <bold>Figure 3:</bold> This figure needs graphical improvement. 
                            <list list-type="bullet">
                                <list-item>
                                    <p>Ensure the copyright of any images used is properly cited or that permission has been obtained.</p>
                                </list-item>
                                <list-item>
                                    <p>If images were generated using AI, please check the journal's policy on the use of AI tools for figures.</p>
                                </list-item>
                            </list> </p>
                    </list-item>
                    <list-item>
                        <p>
                            <bold>Figure 4:</bold> Improve its readability, especially for readers unfamiliar with climate-responsive design tools. 
                            <list list-type="bullet">
                                <list-item>
                                    <p>Make clear if the legend relates to lines or data points.</p>
                                </list-item>
                                <list-item>
                                    <p>Use color-coded arrows or graphical elements to help explain the design strategies.</p>
                                </list-item>
                                <list-item>
                                    <p>The link between the figure and the strategies described below it must be visually reinforced (e.g., using matching colors or icons).</p>
                                </list-item>
                            </list> </p>
                    </list-item>
                </list> </p>
            <p> 
                <bold>3. Methodology and Assumptions</bold> 
                <list list-type="bullet">
                    <list-item>
                        <p>
                            <bold>Material Thickness Choices:</bold> The rationale behind selecting different thicknesses for mycelium (75mm), XPS (70mm), and Rockwool (50mm) is unclear. 
                            <list list-type="bullet">
                                <list-item>
                                    <p>Since all three materials are commercially available at 75mm, a uniform comparison would be more appropriate.</p>
                                </list-item>
                                <list-item>
                                    <p>Alternatively, if you're comparing them based on other premises,&#x00a0;this needs to be explicitly stated and justified.</p>
                                </list-item>
                            </list> </p>
                    </list-item>
                    <list-item>
                        <p>
                            <bold>Wall Assembly Logic:</bold> 
                            <list list-type="bullet">
                                <list-item>
                                    <p>The placement of mycelium in the middle layer and Rockwool on the outer layer must be explained.</p>
                                </list-item>
                                <list-item>
                                    <p>Is this arrangement based on established construction practices? If so, please cite relevant references.</p>
                                </list-item>
                                <list-item>
                                    <p>Otherwise, clarify the reasoning and whether this setup could realistically be implemented in Egyptian construction.</p>
                                </list-item>
                            </list> </p>
                    </list-item>
                </list> 
                <bold>4. Claims That Require Supporting Data</bold> 
                <list list-type="bullet">
                    <list-item>
                        <p>
                            <bold>Carbon Absorption Statement:</bold>
                        </p>
                        <p> 
                            <italic>&#x201c;Mycelium has the capability to capture and store over 16 metric tons of carbon in just one month.&#x201d;</italic> 
                            <list list-type="bullet">
                                <list-item>
                                    <p>This is an extremely bold claim. You must specify: 
                                        <list list-type="bullet">
                                            <list-item>
                                                <p>How much mycelium biomass is required to achieve this carbon sequestration?</p>
                                            </list-item>
                                        </list> </p>
                                </list-item>
                            </list> </p>
                    </list-item>
                    <list-item>
                        <p>
                            <bold>Conclusion Section:</bold>
                        </p>
                        <p> Several claims lack supporting references, such as: 
                            <list list-type="bullet">
                                <list-item>
                                    <p>XPS creating sealed environments prone to mold.</p>
                                </list-item>
                                <list-item>
                                    <p>Biodegradability and fire risks of XPS.</p>
                                </list-item>
                                <list-item>
                                    <p>Indoor air quality degradation.</p>
                                </list-item>
                                <list-item>
                                    <p>Health impacts on building occupants.</p>
                                </list-item>
                                <list-item>
                                    <p>
                                        <bold>Recommendation:</bold> Every statement in this section must be supported by peer-reviewed or technical reference. Avoid repeating unverified assumptions.</p>
                                </list-item>
                            </list> </p>
                    </list-item>
                    <list-item>
                        <p>
                            <bold>Mycelium Advantages (Bullet List):</bold> 
                            <list list-type="bullet">
                                <list-item>
                                    <p>The following claims need references: 
                                        <list list-type="bullet">
                                            <list-item>
                                                <p>Lower cost &#x2013; please include economic comparison per square meter using current market prices.</p>
                                            </list-item>
                                            <list-item>
                                                <p>Biodegradability &#x2013; supported by LCA or composting data.</p>
                                            </list-item>
                                            <list-item>
                                                <p>Low energy in production &#x2013; cite data comparing embodied energy of production.</p>
                                            </list-item>
                                            <list-item>
                                                <p>Limited production locations &#x2013; quantify or describe this constraint and its implications.</p>
                                            </list-item>
                                        </list> </p>
                                </list-item>
                                <list-item>
                                    <p>Overall, the conclusion needs to be grounded in stronger and more comprehensive data.</p>
                                </list-item>
                            </list> </p>
                    </list-item>
                </list> 
                <bold>5. Broader Argument and Contribution</bold>
            </p>
            <p> Currently, the paper shows that mycelium is roughly comparable to XPS in performance. However, it does not clearly build a compelling case for why the construction sector should adopt it. For instance: 
                <list list-type="bullet">
                    <list-item>
                        <p>Is it more sustainable across the entire lifecycle?</p>
                    </list-item>
                    <list-item>
                        <p>Is it more cost-effective?</p>
                    </list-item>
                    <list-item>
                        <p>Is there a regulatory or design advantage?</p>
                    </list-item>
                </list> Without these arguments being data-driven, the conclusion remains speculative. The paper should include a stronger comparative synthesis, perhaps a table summarizing key performance indicators (e.g., thermal conductivity, embodied energy, biodegradability, toxicity, fire safety, cost).</p>
            <p> 
                <bold>Final Recommendation:</bold>
            </p>
            <p> The paper addresses an important topic with promising potential. However, due to issues with clarity, insufficient referencing, methodological gaps, and weak evidence in support of key claims, I recommend a decision once the author makes the necessary changes: 
                <list list-type="bullet">
                    <list-item>
                        <p>Expand and strengthen the literature review,</p>
                    </list-item>
                    <list-item>
                        <p>Justify modeling choices more rigorously,</p>
                    </list-item>
                    <list-item>
                        <p>Substantiate all environmental and performance claims with references,</p>
                    </list-item>
                    <list-item>
                        <p>Improve figure clarity and citation, the paper could make a valuable contribution to the field of sustainable construction materials.</p>
                    </list-item>
                </list>
            </p>
            <p>Is the case presented with sufficient detail to be useful for teaching or other practitioners?</p>
            <p>Partly</p>
            <p>Is the work clearly and accurately presented and does it cite the current literature?</p>
            <p>No</p>
            <p>If applicable, is the statistical analysis and its interpretation appropriate?</p>
            <p>Partly</p>
            <p>Are all the source data underlying the results available to ensure full reproducibility?</p>
            <p>Yes</p>
            <p>Are the conclusions drawn adequately supported by the results?</p>
            <p>Partly</p>
            <p>Is the background of the case&#x2019;s history and progression described in sufficient detail?</p>
            <p>No</p>
            <p>Reviewer Expertise:</p>
            <p>Mycelium materials. Life-cycle assessment and thermal performance</p>
            <p>I confirm that I have read this submission and believe that I have an appropriate level of expertise to confirm that it is of an acceptable scientific standard, however I have significant reservations, as outlined above.</p>
        </body>
        <sub-article article-type="response" id="comment15065-392627">
            <front-stub>
                <contrib-group>
                    <contrib contrib-type="author">
                        <name>
                            <surname>khaled</surname>
                            <given-names>passant</given-names>
                        </name>
                        <aff>Interior, Arab Academy for Science Technology and Maritime Transport College of Engineering and Technology, Alexandria, Alexandria Governorate, Egypt</aff>
                    </contrib>
                </contrib-group>
                <author-notes>
                    <fn fn-type="conflict">
                        <p>
                            <bold>Competing interests: </bold>No competing interests were disclosed.</p>
                    </fn>
                </author-notes>
                <pub-date pub-type="epub">
                    <day>12</day>
                    <month>12</month>
                    <year>2025</year>
                </pub-date>
            </front-stub>
            <body>
                <p>
                    <bold>Reviewer comment:</bold>
                </p>
                <p> 
                    <italic>The paper does not clearly build a compelling case for why the construction sector should adopt mycelium. Several claims in the conclusion lack supporting references</italic>
                </p>
                <p> 
                    <bold>Author response:</bold>
                </p>
                <p> Thank you for this comment. The conclusion has been strengthened by adding additional peer-reviewed and technical references to support key claims. In addition, a new comparative table has been introduced to directly compare mycelium and XPS across indicators of thermal performance, embodied energy, emissions, biodegradability, fire safety, and overall environmental impact, based on published literature.</p>
            </body>
        </sub-article>
    </sub-article>
</article>
