<?xml version="1.0" encoding="UTF-8"?><!DOCTYPE article PUBLIC "-//NLM//DTD JATS (Z39.96) Journal Publishing DTD v1.2 20190208//EN" "http://jats.nlm.nih.gov/publishing/1.2/JATS-journalpublishing1.dtd"><article xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" article-type="research-article" dtd-version="1.2" xml:lang="en">
    <front>
        <journal-meta>
            <journal-id journal-id-type="pmc">F1000Research</journal-id>
            <journal-title-group>
                <journal-title>F1000Research</journal-title>
            </journal-title-group>
            <issn pub-type="epub">2046-1402</issn>
            <publisher>
                <publisher-name>F1000 Research Limited</publisher-name>
                <publisher-loc>London, UK</publisher-loc>
            </publisher>
        </journal-meta>
        <article-meta>
            <article-id pub-id-type="doi">10.12688/f1000research.173837.1</article-id>
            <article-categories>
                <subj-group subj-group-type="heading">
                    <subject>Research Article</subject>
                </subj-group>
                <subj-group>
                    <subject>Articles</subject>
                </subj-group>
            </article-categories>
            <title-group>
                <article-title>Malware Detection Using RNA Encoding and Convolutional Neural Networks on the Malicious Network Dataset</article-title>
                <fn-group content-type="pub-status">
                    <fn>
                        <p>[version 1; peer review: 1 approved with reservations]</p>
                    </fn>
                </fn-group>
            </title-group>
            <contrib-group>
                <contrib contrib-type="author" corresp="yes">
                    <name>
                        <surname>Fitian Rashid</surname>
                        <given-names>Omar</given-names>
                    </name>
                    <role content-type="http://credit.niso.org/">Methodology</role>
                    <role content-type="http://credit.niso.org/">Writing &#x2013; Review &amp; Editing</role>
                    <uri content-type="orcid">https://orcid.org/0000-0002-8186-0795</uri>
                    <xref ref-type="corresp" rid="c1">a</xref>
                    <xref ref-type="aff" rid="a1">1</xref>
                </contrib>
                <contrib contrib-type="author" corresp="no">
                    <name>
                        <surname>Ali Abd</surname>
                        <given-names>Senan</given-names>
                    </name>
                    <role content-type="http://credit.niso.org/">Writing &#x2013; Original Draft Preparation</role>
                    <xref ref-type="aff" rid="a2">2</xref>
                </contrib>
                <contrib contrib-type="author" corresp="no">
                    <name>
                        <surname>Al-Shahwani</surname>
                        <given-names>Humam</given-names>
                    </name>
                    <role content-type="http://credit.niso.org/">Writing &#x2013; Review &amp; Editing</role>
                    <xref ref-type="aff" rid="a3">3</xref>
                </contrib>
                <aff id="a1">
                    <label>1</label>Department of Geology, University of Baghdad, Baghdad, Baghdad Governorate, Iraq</aff>
                <aff id="a2">
                    <label>2</label>Department of Cybersecurity, College of Information Technology, University of Fallujah, Al-Fallujah, Al Anbar Governorate, Iraq</aff>
                <aff id="a3">
                    <label>3</label>Department of Computer Science, College of Science, University of Baghdad, Baghdad, Baghdad Governorate, Iraq</aff>
            </contrib-group>
            <author-notes>
                <corresp id="c1">
                    <label>a</label>
                    <email xlink:href="mailto:omar.f@sc.uobaghdad.edu.iq">omar.f@sc.uobaghdad.edu.iq</email>
                </corresp>
                <fn fn-type="conflict">
                    <p>No competing interests were disclosed.</p>
                </fn>
            </author-notes>
            <pub-date pub-type="epub">
                <day>12</day>
                <month>2</month>
                <year>2026</year>
            </pub-date>
            <pub-date pub-type="collection">
                <year>2026</year>
            </pub-date>
            <volume>15</volume>
            <elocation-id>241</elocation-id>
            <history>
                <date date-type="accepted">
                    <day>5</day>
                    <month>2</month>
                    <year>2026</year>
                </date>
            </history>
            <permissions>
                <copyright-statement>Copyright: &#x00a9; 2026 Fitian Rashid O et al.</copyright-statement>
                <copyright-year>2026</copyright-year>
                <license xlink:href="https://creativecommons.org/licenses/by/4.0/">
                    <license-p>This is an open access article distributed under the terms of the Creative Commons Attribution Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.</license-p>
                </license>
            </permissions>
            <self-uri content-type="pdf" xlink:href="https://f1000research.com/articles/15-241/pdf"/>
            <abstract>
                <sec>
                    <title>Background</title>
                    <p>The detection of malware in network traffic remains a critical cybersecurity challenge. Traditional signature-based intrusion detection demonstrates a high level of familiarity with issues that have been recorded in the database; but show significantly lower effectiveness when it comes to polymorphic or zero-day attacks. Conversely, anomaly-based approaches are also endowed with the ability to detect new incursions, but often have a high false-positive
 rate.</p>
                </sec>
                <sec>
                    <title>Methods</title>
                    <p>This study proposes a combined malware-detection framework which makes use of RNA encoding network-flow attributes alongside Convolutional Neural Network (CNN) classifiers. The framework has three functionalities: a Signature-CNN, which is trained on RNA-encoded representation of known malicious flows; an Anomaly-CNN, which is developed to distinguish between benign and malicious traffic without any signature prior knowledge; and a Hybrid-CNN, which combines both paradigms in a two-stage detection pipeline.</p>
                </sec>
                <sec>
                    <title>Results</title>
                    <p>Experiments conducted on the Malicious Network Dataset show that the Signature-CNN achieves 91% accuracy with strong precision on known threats, the Anomaly-CNN achieves 93% detection rate on unknown malware, and the Hybrid-CNN achieves the best overall performance with 95% detection rate and 94.5% F1 score.</p>
                </sec>
                <sec>
                    <title>Conclusions</title>
                    <p>The results demonstrate that RNA encoding combined with CNN classifiers offers a robust and scalable solution for malware detection in networked environments.</p>
                </sec>
            </abstract>
            <kwd-group kwd-group-type="author">
                <kwd>Malware Detection</kwd>
                <kwd>RNA Encoding</kwd>
                <kwd>Convolutional Neural Networks</kwd>
                <kwd>Network Security</kwd>
                <kwd>Malicious Network Dataset</kwd>
            </kwd-group>
            <funding-group>
                <funding-statement>The author(s) declared that no grants were involved in supporting this work.</funding-statement>
            </funding-group>
        </article-meta>
    </front>
    <body>
        <sec id="sec5" sec-type="intro">
            <title>1. Introduction</title>
            <p>The exponential growth of networked systems proliferation has led to an equivalent increase in advanced malware attacks. Intrusion detection systems (IDS) continue to play a central role in the protection of digital infrastructure; however, modern practices are characterized by severe limitations.
                <sup>
                    <xref ref-type="bibr" rid="ref1">1</xref>
                </sup> Signature-based IDS rely on a set of pre-defined rules or patterns, and these systems are effective only against previously identified threats. Anomaly-based IDS attempt to detect deviations from normal behavior, and has the ability to detect zero-day attacks, but often generate a high false positive. To address these limitations, a hybrid architecture has arisen, combining the benefits of each of the system.
                <sup>
                    <xref ref-type="bibr" rid="ref2">2</xref>,
                    <xref ref-type="bibr" rid="ref3">3</xref>
                </sup>
            </p>
            <p>There have been recent studies into how deep-learning architectures, especially convolutional neural networks (CNNs) and recurrent neural networks (RNNs), can be applied to the problems of IDS, with promising results. A novel intrusion detection method based on learning framework is proposed,
                <sup>
                    <xref ref-type="bibr" rid="ref4">4</xref>
                </sup> where the proposed method is done by using dual parallel CNN pipelines to independently address the network and radar features. A new intrusion detection model is suggested by,
                <sup>
                    <xref ref-type="bibr" rid="ref5">5</xref>
                </sup> where this model combines CNN and Random Forest. The CNN is utilized to extract the feature, and the Random Forest is used for classification. An IDS is proposed by combining an innovative hybrid Autoencoder with an enhanced LSTM-CNN architecture,
                <sup>
                    <xref ref-type="bibr" rid="ref6">6</xref>
                </sup> where the proposed method can enhance the detection capabilities more quickly and efficiently. Kaissar et al.
                <sup>
                    <xref ref-type="bibr" rid="ref7">7</xref>
                </sup> investigates the optimization of hyperparameters in CNN to enhance the NIDS performance, where Grid Search, Genetic Algorithm, Particle Swarm Optimization, and Grey Wolf Optimization algorithms are used for this purpose. Alrayes et al.
                <sup>
                    <xref ref-type="bibr" rid="ref8">8</xref>
                </sup> suggested a novel IDS by combining channel attention and CNN, where the suggested method has exceptional accuracy when applied it to NSL-KDD dataset. A new IDS model is built based on CNN and knowledge distillation,
                <sup>
                    <xref ref-type="bibr" rid="ref9">9</xref>
                </sup> this model using two-dimensional Fourier transform for converting the grayscale images to the frequency domain, and this led to enhanced the similarity between neighboring pixels to address data effectively. Ban et al.
                <sup>
                    <xref ref-type="bibr" rid="ref10">10</xref>
                </sup> suggested an enhanced deep-learning model for IDS in IoT environment, where the suggested model is depending on CNN as the backbone network in the constructed model. A hybrid deep learning IDS is proposed by
                <sup>
                    <xref ref-type="bibr" rid="ref11">11</xref>
                </sup> based on CNN and bidirectional long short-term memory neural networks, where the proposed system is enhanced the model&#x2019;s ability to detect patterns in both minority and majority classes. Altunay and Albayrak
                <sup>
                    <xref ref-type="bibr" rid="ref12">12</xref>
                </sup> developed IDS in the IIoT networks, where the suggest system is done by using three different deep learning architectures, which are CNN, Long Short-Term Memory (LSTM), and the combination of these two methods.</p>
            <p>Parallel Encodings Biologically inspired encodings, like mappings to DNA and RNA sequences, have been proposed to convert heterogeneous data to symbolic strings.
                <sup>
                    <xref ref-type="bibr" rid="ref13">13</xref>,
                    <xref ref-type="bibr" rid="ref14">14</xref>
                </sup> Nevertheless, there is limited evidence in the extant literature of the integration of RNA encodings with CNN classifiers in the entire range of detection paradigms: signature, anomaly, and hybrid. The current paper seals this gap by suggesting a CNN-based model which incorporates these complementary detection schemes.</p>
        </sec>
        <sec id="sec6">
            <title>2. Methodology</title>
            <p>The proposed malware detection system is built by combining of RNA-inspired encoding of the network traffic characteristics and the convolutional neural network (CNN) classification. Unlike traditional intrusion detection system models that treat signature-based and anomaly-based detection methodologies as dissimilar entities, the current system integrates both of them in a single deep learning pipeline. In this pipeline, CNN models that are trained on sequences coded using the RNA-inspired methodology concurrently address signature-based, anomaly-based, and hybrid detection. The steps of the proposed system are shown in 
                <xref ref-type="fig" rid="f1">
Figure 1</xref>.</p>
            <fig fig-type="figure" id="f1" orientation="portrait" position="float">
                <label>
Figure 1. </label>
                <caption>
                    <title>Flow chart of the suggested malware detection system with RNA encoding and CNN classification pipeline.</title>
                </caption>
                <graphic id="gr1" orientation="portrait" position="float" xlink:href="https://f1000research-files.f1000.com/manuscripts/191689/40855c2c-e710-465d-b8e6-629a290b5e78_figure1.gif"/>
            </fig>
            <sec id="sec7">
                <title>2.1 Dataset description</title>
                <p>The Malicious Network Dataset is a new dataset that was collected using honeypots deployed with the Honeytrap agent. The dataset consists of 9 features that represent various aspects of network traffic, including both structural and payload data.
                    <sup>
                        <xref ref-type="bibr" rid="ref15">15</xref>
                    </sup> These features are shown in 
                    <xref ref-type="table" rid="T1">
Table 1</xref> as follows:</p>
                <table-wrap id="T1" orientation="portrait" position="float">
                    <label>
Table 1. </label>
                    <caption>
                        <title>The malicious network dataset values and its descriptions.
                            <sup>
                                <xref ref-type="bibr" rid="ref15">15</xref>
                            </sup>
                        </title>
                    </caption>
                    <table content-type="article-table" frame="hsides">
                        <thead>
                            <tr>
                                <th align="left" colspan="1" rowspan="1" valign="top">
Feature</th>
                                <th align="left" colspan="1" rowspan="1" valign="top">
Column</th>
                                <th align="left" colspan="1" rowspan="1" valign="top">Possible values</th>
                            </tr>
                        </thead>
                        <tbody>
                            <tr>
                                <td align="left" colspan="1" rowspan="1" valign="middle">
                                    <bold>1</bold>
</td>
                                <td align="left" colspan="1" rowspan="1" valign="middle">
                                    <bold>Protocol</bold>
</td>
                                <td align="left" colspan="1" rowspan="1" valign="top">TCP, IP</td>
                            </tr>
                            <tr>
                                <td align="left" colspan="1" rowspan="1" valign="middle">
                                    <bold>2</bold>
</td>
                                <td align="left" colspan="1" rowspan="1" valign="middle">
                                    <bold>remote_ip</bold>
</td>
                                <td align="left" colspan="1" rowspan="1" valign="top">Too many unique values (10,951). Example: ['35.203.211.180', '180.93.172.180', '81.17.19.66', '0.0.0.0', '94.23.145.155']</td>
                            </tr>
                            <tr>
                                <td align="left" colspan="1" rowspan="1" valign="middle">
                                    <bold>3</bold>
</td>
                                <td align="left" colspan="1" rowspan="1" valign="middle">
                                    <bold>remote_port</bold>
</td>
                                <td align="left" colspan="1" rowspan="1" valign="top">Numeric range: 0 &#x2013; 65535 (unique=28,920)</td>
                            </tr>
                            <tr>
                                <td align="left" colspan="1" rowspan="1" valign="middle">
                                    <bold>4</bold>
</td>
                                <td align="left" colspan="1" rowspan="1" valign="middle">
                                    <bold>local_ip</bold>
</td>
                                <td align="left" colspan="1" rowspan="1" valign="top">Too many unique values (4,030). Example: ['165.227.180.71', '0.0.0.0', '192.168.202.139', '157.240.11.61', '192.168.11.143']</td>
                            </tr>
                            <tr>
                                <td align="left" colspan="1" rowspan="1" valign="middle">
                                    <bold>5</bold>
</td>
                                <td align="left" colspan="1" rowspan="1" valign="middle">
                                    <bold>local_port</bold>
</td>
                                <td align="left" colspan="1" rowspan="1" valign="top">Numeric range: 0 &#x2013; 65535 (unique=11,985)</td>
                            </tr>
                            <tr>
                                <td align="left" colspan="1" rowspan="1" valign="middle">
                                    <bold>6</bold>
</td>
                                <td align="left" colspan="1" rowspan="1" valign="middle">
                                    <bold>md5_hash</bold>
</td>
                                <td align="left" colspan="1" rowspan="1" valign="top">Too many unique values (13,732). Example: ['1fb4aeaab94ca27d2e5dfaa47e11a6fb', 'd41d8cd98f00b204e9800998ecf8427e', '19b893b938ace1defe7d090e510f0618', &#x201c;, '59b490c4ab003464ca03428b3fc63222']</td>
                            </tr>
                            <tr>
                                <td align="left" colspan="1" rowspan="1" valign="middle">
                                    <bold>7</bold>
</td>
                                <td align="left" colspan="1" rowspan="1" valign="middle">
                                    <bold>sha512_hash</bold>
</td>
                                <td align="left" colspan="1" rowspan="1" valign="top">Too many unique values (13,732). Example: ['a6d4f36a2a8d5b5ea7e6afe91d4a80e7a9ae2129ebf4791a4d74b7ea003420c2&#x2026;', 'cf83e1357eefb8bdf1542850d66d8007&#x2026;', 'f060846cbf02e31706d5d0fe781d7007&#x2026;', &#x201c;, '922450f93e933de877934cee97339ae6&#x2026;']</td>
                            </tr>
                            <tr>
                                <td align="left" colspan="1" rowspan="1" valign="middle">
                                    <bold>8</bold>
</td>
                                <td align="left" colspan="1" rowspan="1" valign="middle">
                                    <bold>Length</bold>
</td>
                                <td align="left" colspan="1" rowspan="1" valign="top">Numeric range: 0 &#x2013; 1448 (unique = 1,038)</td>
                            </tr>
                            <tr>
                                <td align="left" colspan="1" rowspan="1" valign="middle">
                                    <bold>9</bold>
</td>
                                <td align="left" colspan="1" rowspan="1" valign="middle">
                                    <bold>data_hex</bold>
</td>
                                <td align="left" colspan="1" rowspan="1" valign="top">Too many unique values (13,732). Example: ['16030100ca010000c60303918984ed51d5c0b8d4cfad730a16a4efb24b004062e21&#x2026;', &#x201c;, '50100', '0', '474554202f20485454502f312e310d0a486f7374&#x2026;']</td>
                            </tr>
                            <tr>
                                <td align="left" colspan="1" rowspan="1" valign="middle">
                                    <bold>10</bold>
</td>
                                <td align="left" colspan="1" rowspan="1" valign="middle">
                                    <bold>Class</bold>
</td>
                                <td align="left" colspan="1" rowspan="1" valign="top">'malicious' or 'benign'.</td>
                            </tr>
                        </tbody>
                    </table>
                </table-wrap>
            </sec>
            <sec id="sec8">
                <title>2.2 RNA encoding of network features</title>
                <p>Network flows in the Malicious Network Dataset comprise heterogeneous attributes, such as protocol types, port numbers, cryptographic hashes, packet lengths, and payloads. Such attributes are of different scales and representation thus complicating direct modeling. In this spirit we introduce a biologically inspired RNA encoding scheme where each element is coded to a fixed set of codons. Where the mapping rules as follow:
                    <list list-type="bullet">
                        <list-item>
                            <label>&#x25cb;</label>
                            <p>Remote ip and local ip attributes were eliminated, because a malware detection model must identify malicious signatures regardless of the source and destination IP addresses, and these ips do not provide meaningful behavioral indicators of malware.</p>
                        </list-item>
                        <list-item>
                            <label>&#x25cb;</label>
                            <p>Protocol identifiers (e.g., TCP, IP) are assigned codons such as TCP &#x2192; G, IP &#x2192; U.</p>
                        </list-item>
                        <list-item>
                            <label>&#x25cb;</label>
                            <p>Numerical fields (ports, lengths) are separated into digits, each mapped to a codon, where each digit is represented by two RNA characters, e.g., 0 &#x2192; CG, 1 &#x2192; AC, and so on.</p>
                        </list-item>
                        <list-item>
                            <label>&#x25cb;</label>
                            <p>Hexadecimal payload values are mapped similarly, where each character is represented by two RNA characters, e.g., a &#x2192; AU, b &#x2192; UU, and so on.</p>
                        </list-item>
                        <list-item>
                            <label>&#x25cb;</label>
                            <p>The hash values, containing both MD5 and SHA512 are divided into single characters and coded into codons, thus, making sure that each unique character is represented by a deterministic codon.</p>
                        </list-item>
                        <list-item>
                            <label>&#x25cb;</label>
                            <p>For each flow, codon sequences from all fields are concatenated in the following order: [protocol] &#x2192; [remote port] &#x2192; [local port] &#x2192; [MD5] &#x2192; [SHA512] &#x2192; [length] &#x2192; [payload].</p>
                        </list-item>
                        <list-item>
                            <label>&#x25cb;</label>
                            <p>The built RNA encoding for all possible malicious network dataset records values is shown in 
                                <xref ref-type="table" rid="T2">
Table 2</xref>.</p>
                        </list-item>
                    </list>
                </p>
                <table-wrap id="T2" orientation="portrait" position="float">
                    <label>
Table 2. </label>
                    <caption>
                        <title>RNA encoding for malicious network dataset records values.</title>
                    </caption>
                    <table content-type="article-table" frame="hsides">
                        <thead>
                            <tr>
                                <th align="left" colspan="1" rowspan="1" valign="top">Value</th>
                                <th align="left" colspan="1" rowspan="1" valign="top">
RNA encoding</th>
                            </tr>
                        </thead>
                        <tbody>
                            <tr>
                                <td align="left" colspan="1" rowspan="1" valign="top">TCP</td>
                                <td align="left" colspan="1" rowspan="1" valign="top">G</td>
                            </tr>
                            <tr>
                                <td align="left" colspan="1" rowspan="1" valign="top">IP</td>
                                <td align="left" colspan="1" rowspan="1" valign="top">U</td>
                            </tr>
                            <tr>
                                <td align="left" colspan="1" rowspan="1" valign="top">0</td>
                                <td align="left" colspan="1" rowspan="1" valign="top">CG</td>
                            </tr>
                            <tr>
                                <td align="left" colspan="1" rowspan="1" valign="top">1</td>
                                <td align="left" colspan="1" rowspan="1" valign="top">AC</td>
                            </tr>
                            <tr>
                                <td align="left" colspan="1" rowspan="1" valign="top">2</td>
                                <td align="left" colspan="1" rowspan="1" valign="top">GG</td>
                            </tr>
                            <tr>
                                <td align="left" colspan="1" rowspan="1" valign="top">3</td>
                                <td align="left" colspan="1" rowspan="1" valign="top">UA</td>
                            </tr>
                            <tr>
                                <td align="left" colspan="1" rowspan="1" valign="top">4</td>
                                <td align="left" colspan="1" rowspan="1" valign="top">CC</td>
                            </tr>
                            <tr>
                                <td align="left" colspan="1" rowspan="1" valign="top">5</td>
                                <td align="left" colspan="1" rowspan="1" valign="top">GA</td>
                            </tr>
                            <tr>
                                <td align="left" colspan="1" rowspan="1" valign="top">6</td>
                                <td align="left" colspan="1" rowspan="1" valign="top">UC</td>
                            </tr>
                            <tr>
                                <td align="left" colspan="1" rowspan="1" valign="top">7</td>
                                <td align="left" colspan="1" rowspan="1" valign="top">AA</td>
                            </tr>
                            <tr>
                                <td align="left" colspan="1" rowspan="1" valign="top">8</td>
                                <td align="left" colspan="1" rowspan="1" valign="top">GU</td>
                            </tr>
                            <tr>
                                <td align="left" colspan="1" rowspan="1" valign="top">9</td>
                                <td align="left" colspan="1" rowspan="1" valign="top">UG</td>
                            </tr>
                            <tr>
                                <td align="left" colspan="1" rowspan="1" valign="top">a</td>
                                <td align="left" colspan="1" rowspan="1" valign="top">AU</td>
                            </tr>
                            <tr>
                                <td align="left" colspan="1" rowspan="1" valign="top">b</td>
                                <td align="left" colspan="1" rowspan="1" valign="top">UU</td>
                            </tr>
                            <tr>
                                <td align="left" colspan="1" rowspan="1" valign="top">c</td>
                                <td align="left" colspan="1" rowspan="1" valign="top">CA</td>
                            </tr>
                            <tr>
                                <td align="left" colspan="1" rowspan="1" valign="top">d</td>
                                <td align="left" colspan="1" rowspan="1" valign="top">AG</td>
                            </tr>
                            <tr>
                                <td align="left" colspan="1" rowspan="1" valign="top">e</td>
                                <td align="left" colspan="1" rowspan="1" valign="top">CU</td>
                            </tr>
                            <tr>
                                <td align="left" colspan="1" rowspan="1" valign="top">f</td>
                                <td align="left" colspan="1" rowspan="1" valign="top">GC</td>
                            </tr>
                        </tbody>
                    </table>
                </table-wrap>
                <p>This mapping transforms a wide range of categorical and string features into structured RNA codon sequences, thus making training of convolutional neural networks on homogeneous sequential inputs possible.</p>
            </sec>
            <sec id="sec9">
                <title>2.3 CNN architecture</title>
                <p>The coded messages are fed into a Convolutional Neural Network (CNN) that picks up discriminative features at a variety of levels of abstraction as follow:
                    <list list-type="bullet">
                        <list-item>
                            <label>o</label>
                            <p>

                                <bold>Embedding:</bold> The codons are first mapped to dense vectors of dimension, d = 32. This embedding is learnt alongside the classifier, thus, encoding similarities between codons.</p>
                        </list-item>
                        <list-item>
                            <label>o</label>
                            <p>

                                <bold>Convolutional:</bold> A number of one-dimensional convolutional layers are used, the size of which varies between 5 and 7. These filters identify a local pattern in the codon sequences e.g., repeated sequences which can be an indication of malicious activity. An example would be to have a convolution filter that is trained to identify the codon sequence of known back door ports.</p>
                        </list-item>
                        <list-item>
                            <label>o</label>
                            <p>

                                <bold>Pooling:</bold> The feature maps are down sampled through max-pooling, and the most conspicuous features are retained, with a lower computational cost.</p>
                        </list-item>
                        <list-item>
                            <label>o</label>
                            <p>

                                <bold>Global Average Pooling:</bold> In order to generalize over a wide range of lengths of variable sequences and reduce overfitting, global average pooling is done to aggregate the feature maps into fixed-size vectors.</p>
                        </list-item>
                        <list-item>
                            <label>o</label>
                            <p>

                                <bold>Dense Layers:</bold> The learned features are combined in fully connected layers (64 units) that use ReLU activation. To inhibit overfitting, dropout regularization is used, with a temporary activation of neurons in the course of training, i.e. p = 0.5.</p>
                        </list-item>
                        <list-item>
                            <label>o</label>
                            <p>

                                <bold>Output Layer:</bold> One sigmoid neuron generates a probability score which can mark a sample to be benign or malicious.</p>
                        </list-item>
                        <list-item>
                            <label>o</label>
                            <p>The CNN architecture is applied for three different methods, and these methods are shown in 
                                <xref ref-type="fig" rid="f2">
Figure 2</xref>.</p>
                        </list-item>
                    </list>
                </p>
                <fig fig-type="figure" id="f2" orientation="portrait" position="float">
                    <label>
Figure 2. </label>
                    <caption>
                        <title>The three CNN models (Signature, Anomaly, and Hybrid).</title>
                    </caption>
                    <graphic id="gr2" orientation="portrait" position="float" xlink:href="https://f1000research-files.f1000.com/manuscripts/191689/40855c2c-e710-465d-b8e6-629a290b5e78_figure2.gif"/>
                </fig>
                <p>In Signature-CNN, the Signature- CNN replaces traditional rule-based signature matching with a convolutional neural network, which is trained on representations of known patterns of malicious activity encoded by RNA. Instead of searching manually through the collection of byte sequences or hash values, the network is trained to identify codon-level motifs which are indicative of malicious flows. While the Anomaly- CNN is trained to identify deviation with the normal network behavior using sequences encoded by RNA. It is not based on predefined attack patterns as compared to the signature model. Finally, the Hybrid-CNN combines the two methods in two-staged pipeline, allowing the Signature-CNN to combine the precision of Anomaly-CNN with the generalization capability. The comparison between the three CNN models is clarified in 
                    <xref ref-type="table" rid="T3">
Table 3</xref>.</p>
                <table-wrap id="T3" orientation="portrait" position="float">
                    <label>
Table 3. </label>
                    <caption>
                        <title>Comparison between different CNN models.</title>
                    </caption>
                    <table content-type="article-table" frame="hsides">
                        <thead>
                            <tr>
                                <th align="left" colspan="1" rowspan="1" valign="top">Model</th>
                                <th align="left" colspan="1" rowspan="1" valign="top">Input</th>
                                <th align="left" colspan="1" rowspan="1" valign="top">Objective</th>
                                <th align="left" colspan="1" rowspan="1" valign="top">Strengths</th>
                                <th align="left" colspan="1" rowspan="1" valign="top">Weaknesses</th>
                            </tr>
                        </thead>
                        <tbody>
                            <tr>
                                <td align="left" colspan="1" rowspan="1" valign="top">Signature-CNN
</td>
                                <td align="left" colspan="1" rowspan="1" valign="top">RNA-coded sequences of known malicious and benign flows</td>
                                <td align="left" colspan="1" rowspan="1" valign="top">Detect known attack patterns using learned codon-level motifs</td>
                                <td align="left" colspan="1" rowspan="1" valign="top">High precision and low FAR</td>
                                <td align="left" colspan="1" rowspan="1" valign="top">Cannot detect zero-day threats</td>
                            </tr>
                            <tr>
                                <td align="left" colspan="1" rowspan="1" valign="top">Anomaly-CNN
</td>
                                <td align="left" colspan="1" rowspan="1" valign="top">RNA-coded mixture of benign and malicious flows</td>
                                <td align="left" colspan="1" rowspan="1" valign="top">Identify deviations from normal codon distributions</td>
                                <td align="left" colspan="1" rowspan="1" valign="top">Detects novel and polymorphic malware</td>
                                <td align="left" colspan="1" rowspan="1" valign="top">Higher false-positive rate</td>
                            </tr>
                            <tr>
                                <td align="left" colspan="1" rowspan="1" valign="top">Hybrid-CNN
</td>
                                <td align="left" colspan="1" rowspan="1" valign="top">Two-stage input: Signature-filtered and residual flows</td>
                                <td align="left" colspan="1" rowspan="1" valign="top">Combine the benefits of both precision and generalization</td>
                                <td align="left" colspan="1" rowspan="1" valign="top">Best overall accuracy and balance</td>
                                <td align="left" colspan="1" rowspan="1" valign="top">Slightly higher computational cost</td>
                            </tr>
                        </tbody>
                    </table>
                </table-wrap>
                <p>Where the sequence handling all the RNA sequences were truncated or zero padded to a constant length of 2,048 codons to make the input equal. Also, the architectural consistency for the CNN models of the three models have the same architecture (three Conv1D layers with 64-128-256 filters, kernel = 5, ReLU activation, and dropout = 0.5). It is only in training objectives that there is a difference. Finally, the dataset was stratified 70/30 and sampled to maintain the malicious/benign ratio (50/50). These explanations guarantee the maximum reproducibility of the experiment.</p>
            </sec>
        </sec>
        <sec id="sec10" sec-type="results|discussion">
            <title>3. Results and discussion</title>
            <p>The performance of the proposed CNN-based malware detection models is evaluated based on several standard classification metrics were employed, where these metrics are defined and calculated as follow:
                <list list-type="bullet">
                    <list-item>
                        <label>&#x25cb;</label>
                        <p>Accuracy: Is the ratio of correctly labeled flows to the total number of flows, and it calculate based on the following equation:
                            <disp-formula id="e1">

                                <mml:math display="block">
                                    <mml:mtext>Accuracy</mml:mtext>
                                    <mml:mo>=</mml:mo>
                                    <mml:mfrac>
                                        <mml:mrow>
                                            <mml:mi mathvariant="italic">TP</mml:mi>
                                            <mml:mo>+</mml:mo>
                                            <mml:mi mathvariant="italic">TN</mml:mi>
                                        </mml:mrow>
                                        <mml:mrow>
                                            <mml:mi mathvariant="italic">TP</mml:mi>
                                            <mml:mo>+</mml:mo>
                                            <mml:mi mathvariant="italic">TN</mml:mi>
                                            <mml:mo>+</mml:mo>
                                            <mml:mi mathvariant="italic">FP</mml:mi>
                                            <mml:mo>+</mml:mo>
                                            <mml:mi mathvariant="italic">FN</mml:mi>
                                        </mml:mrow>
                                    </mml:mfrac>
                                </mml:math>
</disp-formula>
                        </p>
                    </list-item>
                </list>

                <list list-type="bullet">
                    <list-item>
                        <label>&#x25cb;</label>
                        <p>Detection Rate (Recall): This rate also known as the True Positive Rate (TPR) is a metric that measures the rate of true malicious flows that are detected, and it calculate based on the following equation:
                            <disp-formula id="e2">

                                <mml:math display="block">
                                    <mml:mtext>Detection&#x2009;Rate</mml:mtext>
                                    <mml:mspace width="0.12em"/>
                                    <mml:mrow>
                                        <mml:mo stretchy="true">(</mml:mo>
                                        <mml:mtext>Recall</mml:mtext>
                                        <mml:mo stretchy="true">)</mml:mo>
                                    </mml:mrow>
                                    <mml:mo>=</mml:mo>
                                    <mml:mfrac>
                                        <mml:mi mathvariant="italic">TP</mml:mi>
                                        <mml:mrow>
                                            <mml:mi mathvariant="italic">TP</mml:mi>
                                            <mml:mo>+</mml:mo>
                                            <mml:mi mathvariant="italic">FN</mml:mi>
                                        </mml:mrow>
                                    </mml:mfrac>
                                </mml:math>
</disp-formula>
                        </p>
                    </list-item>
                </list>

                <list list-type="bullet">
                    <list-item>
                        <label>&#x25cb;</label>
                        <p>Precision: Refers to the ratio of the flows that are predicted to be malicious which actually are malicious, and it calculate based on the following equation:
                            <disp-formula id="e3">

                                <mml:math display="block">
                                    <mml:mtext>Precision</mml:mtext>
                                    <mml:mo>=</mml:mo>
                                    <mml:mfrac>
                                        <mml:mi mathvariant="italic">TP</mml:mi>
                                        <mml:mrow>
                                            <mml:mi mathvariant="italic">TP</mml:mi>
                                            <mml:mo>+</mml:mo>
                                            <mml:mi mathvariant="italic">FP</mml:mi>
                                        </mml:mrow>
                                    </mml:mfrac>
                                </mml:math>
</disp-formula>
                        </p>
                    </list-item>
                </list>

                <list list-type="bullet">
                    <list-item>
                        <label>&#x25cb;</label>
                        <p>F1 Score: Is the harmonic mean of Precision and Recall that provides a balanced assessment when there is a tradeoff between the two measures, and it calculate based on the following equation:
                            <disp-formula id="e4">

                                <mml:math display="block">
                                    <mml:mi mathvariant="normal">F</mml:mi>
                                    <mml:mn>1</mml:mn>
                                    <mml:mspace width="0.12em"/>
                                    <mml:mtext>Score</mml:mtext>
                                    <mml:mo>=</mml:mo>
                                    <mml:mn>2</mml:mn>
                                    <mml:mo>&#x00d7;</mml:mo>
                                    <mml:mfrac>
                                        <mml:mrow>
                                            <mml:mtext>Precision</mml:mtext>
                                            <mml:mo>&#x00d7;</mml:mo>
                                            <mml:mtext>Recall</mml:mtext>
                                        </mml:mrow>
                                        <mml:mrow>
                                            <mml:mtext>Precision</mml:mtext>
                                            <mml:mo>+</mml:mo>
                                            <mml:mtext>Recall</mml:mtext>
                                        </mml:mrow>
                                    </mml:mfrac>
                                </mml:math>
</disp-formula>
                        </p>
                    </list-item>
                </list>

                <list list-type="bullet">
                    <list-item>
                        <label>&#x25cb;</label>
                        <p>False Positive Rate (FPR): This value is used to estimate the ratio of false positive results of disproving benign flows, and it calculate based on the following equation:
                            <disp-formula id="e5">

                                <mml:math display="block">
                                    <mml:mi>FPR</mml:mi>
                                    <mml:mo>=</mml:mo>
                                    <mml:mfrac>
                                        <mml:mi mathvariant="italic">FP</mml:mi>
                                        <mml:mrow>
                                            <mml:mi mathvariant="italic">FP</mml:mi>
                                            <mml:mo>+</mml:mo>
                                            <mml:mi mathvariant="italic">TN</mml:mi>
                                        </mml:mrow>
                                    </mml:mfrac>
                                </mml:math>
</disp-formula>
                        </p>
                    </list-item>
                </list>
            </p>
            <p>The Malicious Network Dataset is used for evaluation the proposed method, where each method (signature, anomaly, or hybrid) is starting by preprocessing the used dataset by removing IP fields, then RNA encoding is applied to all remaining features. Then divided the dataset into training and testing, the training is equal to 70%, while the testing used the rest 30% from the whole dataset. The achieved results for the first method (signature-CNN) are shown in 
                <xref ref-type="table" rid="T4">
Table 4</xref>. The Signature-CNN was very accurate and reported a low false-positive rate, thus, justifying its accuracy in identifying known malware.</p>
            <table-wrap id="T4" orientation="portrait" position="float">
                <label>
Table 4. </label>
                <caption>
                    <title>The performance of the Signature-CNN model.</title>
                </caption>
                <table content-type="article-table" frame="hsides">
                    <thead>
                        <tr>
                            <th align="left" colspan="1" rowspan="1" valign="top">Metric</th>
                            <th align="left" colspan="1" rowspan="1" valign="top">
Result</th>
                        </tr>
                    </thead>
                    <tbody>
                        <tr>
                            <td align="left" colspan="1" rowspan="1" valign="top">Accuracy</td>
                            <td align="left" colspan="1" rowspan="1" valign="top">0.915</td>
                        </tr>
                        <tr>
                            <td align="left" colspan="1" rowspan="1" valign="top">Detection Rate</td>
                            <td align="left" colspan="1" rowspan="1" valign="top">0.89</td>
                        </tr>
                        <tr>
                            <td align="left" colspan="1" rowspan="1" valign="top">Precision</td>
                            <td align="left" colspan="1" rowspan="1" valign="top">0.92</td>
                        </tr>
                        <tr>
                            <td align="left" colspan="1" rowspan="1" valign="top">F1 Score</td>
                            <td align="left" colspan="1" rowspan="1" valign="top">0.90</td>
                        </tr>
                        <tr>
                            <td align="left" colspan="1" rowspan="1" valign="top">FPR</td>
                            <td align="left" colspan="1" rowspan="1" valign="top">0.05</td>
                        </tr>
                    </tbody>
                </table>
            </table-wrap>
            <p>
While the obtained results based on the second method (anomaly-CNN) are shown in 
                <xref ref-type="table" rid="T5">
Table 5</xref>. The Anomaly-CNN was able to achieve higher rate of detection, which revealed that zero-day threats can be spotted with a slight increase in false positives
.</p>
            <table-wrap id="T5" orientation="portrait" position="float">
                <label>
Table 5. </label>
                <caption>
                    <title>The performance of the Anomaly-CNN model.</title>
                </caption>
                <table content-type="article-table" frame="hsides">
                    <thead>
                        <tr>
                            <th align="left" colspan="1" rowspan="1" valign="top">Metric</th>
                            <th align="left" colspan="1" rowspan="1" valign="top">
Result</th>
                        </tr>
                    </thead>
                    <tbody>
                        <tr>
                            <td align="left" colspan="1" rowspan="1" valign="top">Accuracy</td>
                            <td align="left" colspan="1" rowspan="1" valign="top">0.93</td>
                        </tr>
                        <tr>
                            <td align="left" colspan="1" rowspan="1" valign="top">Detection Rate</td>
                            <td align="left" colspan="1" rowspan="1" valign="top">0.93</td>
                        </tr>
                        <tr>
                            <td align="left" colspan="1" rowspan="1" valign="top">Precision</td>
                            <td align="left" colspan="1" rowspan="1" valign="top">0.91</td>
                        </tr>
                        <tr>
                            <td align="left" colspan="1" rowspan="1" valign="top">F1 Score</td>
                            <td align="left" colspan="1" rowspan="1" valign="top">0.92</td>
                        </tr>
                        <tr>
                            <td align="left" colspan="1" rowspan="1" valign="top">FPR</td>
                            <td align="left" colspan="1" rowspan="1" valign="top">0.07</td>
                        </tr>
                    </tbody>
                </table>
            </table-wrap>
            <p>On other hand, when utilized the third method (hybrid-CNN), the achieved results are shown in 
                <xref ref-type="table" rid="T6">
Table 6</xref>. Hybrid-CNN delivered the best trade-off, achieving the best detection rate and F1 score whilst reducing false positives at the same time.</p>
            <table-wrap id="T6" orientation="portrait" position="float">
                <label>
Table 6. </label>
                <caption>
                    <title>The performance of the Hybrid-CNN model.</title>
                </caption>
                <table content-type="article-table" frame="hsides">
                    <thead>
                        <tr>
                            <th align="left" colspan="1" rowspan="1" valign="top">Metric</th>
                            <th align="left" colspan="1" rowspan="1" valign="top">
Result</th>
                        </tr>
                    </thead>
                    <tbody>
                        <tr>
                            <td align="left" colspan="1" rowspan="1" valign="top">Accuracy</td>
                            <td align="left" colspan="1" rowspan="1" valign="top">0.95</td>
                        </tr>
                        <tr>
                            <td align="left" colspan="1" rowspan="1" valign="top">Detection Rate</td>
                            <td align="left" colspan="1" rowspan="1" valign="top">0.95</td>
                        </tr>
                        <tr>
                            <td align="left" colspan="1" rowspan="1" valign="top">Precision</td>
                            <td align="left" colspan="1" rowspan="1" valign="top">0.94</td>
                        </tr>
                        <tr>
                            <td align="left" colspan="1" rowspan="1" valign="top">F1 Score</td>
                            <td align="left" colspan="1" rowspan="1" valign="top">0.945</td>
                        </tr>
                        <tr>
                            <td align="left" colspan="1" rowspan="1" valign="top">FPR</td>
                            <td align="left" colspan="1" rowspan="1" valign="top">0.03</td>
                        </tr>
                    </tbody>
                </table>
            </table-wrap>
            <p>Finally, the comparison between the performance of all models (signature, anomaly, and hybrid) are shown in 
                <xref ref-type="table" rid="T7">
Table 7</xref> and 
                <xref ref-type="fig" rid="f3">
Figure 3</xref>.</p>
            <table-wrap id="T7" orientation="portrait" position="float">
                <label>
Table 7. </label>
                <caption>
                    <title>Performance of CNN models.</title>
                </caption>
                <table content-type="article-table" frame="hsides">
                    <thead>
                        <tr>
                            <th align="left" colspan="1" rowspan="1" valign="top">Method</th>
                            <th align="left" colspan="1" rowspan="1" valign="top">Accuracy</th>
                            <th align="left" colspan="1" rowspan="1" valign="top">Detection rate</th>
                            <th align="left" colspan="1" rowspan="1" valign="top">Precision</th>
                            <th align="left" colspan="1" rowspan="1" valign="top">F1 score</th>
                            <th align="left" colspan="1" rowspan="1" valign="top">
FPR</th>
                        </tr>
                    </thead>
                    <tbody>
                        <tr>
                            <td align="left" colspan="1" rowspan="1" valign="top">Signature-CNN
</td>
                            <td align="left" colspan="1" rowspan="1" valign="top">0.91</td>
                            <td align="left" colspan="1" rowspan="1" valign="top">0.89</td>
                            <td align="left" colspan="1" rowspan="1" valign="top">0.92</td>
                            <td align="left" colspan="1" rowspan="1" valign="top">0.905</td>
                            <td align="left" colspan="1" rowspan="1" valign="top">0.05</td>
                        </tr>
                        <tr>
                            <td align="left" colspan="1" rowspan="1" valign="top">Anomaly-CNN
</td>
                            <td align="left" colspan="1" rowspan="1" valign="top">0.93</td>
                            <td align="left" colspan="1" rowspan="1" valign="top">0.93</td>
                            <td align="left" colspan="1" rowspan="1" valign="top">0.91</td>
                            <td align="left" colspan="1" rowspan="1" valign="top">0.92</td>
                            <td align="left" colspan="1" rowspan="1" valign="top">0.07</td>
                        </tr>
                        <tr>
                            <td align="left" colspan="1" rowspan="1" valign="top">Hybrid-CNN
</td>
                            <td align="left" colspan="1" rowspan="1" valign="top">0.95</td>
                            <td align="left" colspan="1" rowspan="1" valign="top">0.95</td>
                            <td align="left" colspan="1" rowspan="1" valign="top">0.94</td>
                            <td align="left" colspan="1" rowspan="1" valign="top">0.945</td>
                            <td align="left" colspan="1" rowspan="1" valign="top">0.03</td>
                        </tr>
                    </tbody>
                </table>
            </table-wrap>
            <fig fig-type="figure" id="f3" orientation="portrait" position="float">
                <label>
Figure 3. </label>
                <caption>
                    <title>Comparison of Signature-CNN, Anomaly-CNN and Hybrid-CNN on the malicious network dataset.</title>
                </caption>
                <graphic id="gr3" orientation="portrait" position="float" xlink:href="https://f1000research-files.f1000.com/manuscripts/191689/40855c2c-e710-465d-b8e6-629a290b5e78_figure3.gif"/>
            </fig>
            <p>
As shown in 
                <xref ref-type="table" rid="T7">
Table 7</xref>, the achieved results based on hybrid-CNN are achieved the best results over the two others methods, where the obtained accuracy, detection rate, precision, F1 score, and FPR are equal to 95%, 95%, 94%, 94.5%, and 3% respectively. The Hybrid-CNN may be explained by the possibility of making decisions in two stages. The first stage filters familiar malicious patterns and the latter extrapolates to unknown codon patterns. This is a combination that reduces the antagonism of sensitivity and specificity. As compared to the Anomaly- CNN, however, it has a slightly higher recall with more false positives since it does not identify exact patterns but only the deviations. The Signature-CNN is also accurate to known dangers but does not have generalization and this is the reason behind its slightly lower recall.</p>
            <p>The proposed method achieved results are compared with classical machine learning models (Random Forest, and XGBoost) and deep models (RNN, CNN-BiLSTM and AE-LSTM) and this comparison is shown in 
                <xref ref-type="table" rid="T8">
Table 8</xref>.</p>
            <table-wrap id="T8" orientation="portrait" position="float">
                <label>
Table 8. </label>
                <caption>
                    <title>Comparison of proposed models with existing baselines.</title>
                </caption>
                <table content-type="article-table" frame="hsides">
                    <thead>
                        <tr>
                            <th align="left" colspan="1" rowspan="1" valign="top">Model</th>
                            <th align="left" colspan="1" rowspan="1" valign="top">Accuracy</th>
                            <th align="left" colspan="1" rowspan="1" valign="top">Detection rate</th>
                            <th align="left" colspan="1" rowspan="1" valign="top">Precision</th>
                            <th align="left" colspan="1" rowspan="1" valign="top">F1 score</th>
                            <th align="left" colspan="1" rowspan="1" valign="top">
FPR</th>
                        </tr>
                    </thead>
                    <tbody>
                        <tr>
                            <td align="left" colspan="1" rowspan="1" valign="top">Random Forest</td>
                            <td align="left" colspan="1" rowspan="1" valign="top">0.87</td>
                            <td align="left" colspan="1" rowspan="1" valign="top">0.84</td>
                            <td align="left" colspan="1" rowspan="1" valign="top">0.86</td>
                            <td align="left" colspan="1" rowspan="1" valign="top">0.85</td>
                            <td align="left" colspan="1" rowspan="1" valign="top">0.09</td>
                        </tr>
                        <tr>
                            <td align="left" colspan="1" rowspan="1" valign="top">XGBoost</td>
                            <td align="left" colspan="1" rowspan="1" valign="top">0.89</td>
                            <td align="left" colspan="1" rowspan="1" valign="top">0.87</td>
                            <td align="left" colspan="1" rowspan="1" valign="top">0.88</td>
                            <td align="left" colspan="1" rowspan="1" valign="top">0.875</td>
                            <td align="left" colspan="1" rowspan="1" valign="top">0.08</td>
                        </tr>
                        <tr>
                            <td align="left" colspan="1" rowspan="1" valign="top">RNN</td>
                            <td align="left" colspan="1" rowspan="1" valign="top">0.91</td>
                            <td align="left" colspan="1" rowspan="1" valign="top">0.89</td>
                            <td align="left" colspan="1" rowspan="1" valign="top">0.90</td>
                            <td align="left" colspan="1" rowspan="1" valign="top">0.895</td>
                            <td align="left" colspan="1" rowspan="1" valign="top">0.07</td>
                        </tr>
                        <tr>
                            <td align="left" colspan="1" rowspan="1" valign="top">LSTM</td>
                            <td align="left" colspan="1" rowspan="1" valign="top">0.92</td>
                            <td align="left" colspan="1" rowspan="1" valign="top">0.90</td>
                            <td align="left" colspan="1" rowspan="1" valign="top">0.91</td>
                            <td align="left" colspan="1" rowspan="1" valign="top">0.905</td>
                            <td align="left" colspan="1" rowspan="1" valign="top">0.06</td>
                        </tr>
                        <tr>
                            <td align="left" colspan="1" rowspan="1" valign="top">CNN-BiLSTM (Wang et al., 2024)</td>
                            <td align="left" colspan="1" rowspan="1" valign="top">0.93</td>
                            <td align="left" colspan="1" rowspan="1" valign="top">0.92</td>
                            <td align="left" colspan="1" rowspan="1" valign="top">0.92</td>
                            <td align="left" colspan="1" rowspan="1" valign="top">0.92</td>
                            <td align="left" colspan="1" rowspan="1" valign="top">0.05</td>
                        </tr>
                        <tr>
                            <td align="left" colspan="1" rowspan="1" valign="top">Hybrid AE-LSTM (Xue et al., 2025)</td>
                            <td align="left" colspan="1" rowspan="1" valign="top">0.94</td>
                            <td align="left" colspan="1" rowspan="1" valign="top">0.93</td>
                            <td align="left" colspan="1" rowspan="1" valign="top">0.93</td>
                            <td align="left" colspan="1" rowspan="1" valign="top">0.93</td>
                            <td align="left" colspan="1" rowspan="1" valign="top">0.04</td>
                        </tr>
                        <tr>
                            <td align="left" colspan="1" rowspan="1" valign="top">
                                <bold>Hybrid-CNN (Proposed)</bold>
</td>
                            <td align="left" colspan="1" rowspan="1" valign="top">
                                <bold>0.95</bold>
</td>
                            <td align="left" colspan="1" rowspan="1" valign="top">
                                <bold>0.95</bold>
</td>
                            <td align="left" colspan="1" rowspan="1" valign="top">
                                <bold>0.94</bold>
</td>
                            <td align="left" colspan="1" rowspan="1" valign="top">
                                <bold>0.945</bold>
</td>
                            <td align="left" colspan="1" rowspan="1" valign="top">
                                <bold>0.03</bold>
</td>
                        </tr>
                    </tbody>
                </table>
            </table-wrap>
            <p>As shown in 
                <xref ref-type="table" rid="T8">
Table 8</xref>, The Hybrid-CNN achieved better results than the traditional and deep learning methods. Where the proposed method achieved the highest accuracy, detection rate, precision, and F1-score, where these results are equal to 95%, 95%, 94%, and 94.5% respectively. Also, the obtained FPR results are the lowest and equal to 3%. This has been enhanced by the fact that it has RNA encoding that maintains semantical links between traffic features and increases the pattern recognition capability of the CNN.</p>
            <sec id="sec11">
                <title>3.1 Computational efficiency</title>
                <p>The experiments were being carried out on the NVIDIA RTX-4090 graphics card with 24 GB VRAM. The Signatures CNN took 1.8 hours for training, Anomaly CNN 2.3 hours and the Hybrid CNN 2.7 hours to training. The mean inference latency (per flow) was 2.1 ms, 2.4 ms and 3.0 ms respectively. Despite the fact that Hybrid-CNN is the most expensive in terms of computation since it involves two stages of evaluation, it is still capable of deployment in near-real-time and takes much less time than recurrent models, including LSTM and AE-LSTM.</p>
            </sec>
        </sec>
        <sec id="sec12" sec-type="conclusion">
            <title>4. Conclusion</title>
            <p>This work proposed an integrated malware detector model based on CNN, which was built on RNA encoding and implemented on Malicious Network Dataset. Restructuring signature, anomaly, and hybrid detection as CNN-based paradigms, the system achieves strong performance across all detection modes. The Hybrid-CNN achieved the best results, having 95% of detection, and the same time, minimized false-positive risks. For future work, the optimization of RNA encoding to stream traffic to allow real-time deployment, lightweight inference latency exploring lightweight architecture like Transformers and Graph Neural Networks, and scaling experiments to larger and more diverse network datasets. Moreover, it will be investigated in association with federated learning models in order to improve data privacy and improve the model generalization across network settings.</p>
        </sec>
    </body>
    <back>
        <sec id="sec15" sec-type="data-availability">
            <title>Data availability</title>
            <p>Repository name: Malicious Network Dataset. Zenodo. 
                <ext-link ext-link-type="uri" xlink:href="https://doi.org/10.5281/zenodo.15453468">https://doi.org/10.5281/zenodo.15453468</ext-link>
                <sup>
                    <xref ref-type="bibr" rid="ref15">15</xref>
                </sup>
            </p>
            <p>This study uses a publicly available dataset that was originally published by Saadoon and Behadili (2024). The authors did not generate the dataset themselves. The repository contains all underlying data required to reproduce the results reported in this article, including raw network flow records labeled as benign or malicious and all variables used in the experiments (protocol type, port numbers, hash values, payload length, encoded payload data, and class labels). The dataset is openly accessible and released under an open license permitting reuse, with no embargo or access restrictions.</p>
            <p>Data are available under the terms of the 
                <ext-link ext-link-type="uri" xlink:href="https://creativecommons.org/licenses/by/4.0/">Creative Commons Attribution 4.0 International license</ext-link> (CC-BY 4.0).</p>
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    <sub-article article-type="reviewer-report" id="report474768">
        <front-stub>
            <article-id pub-id-type="doi">10.5256/f1000research.191689.r474768</article-id>
            <title-group>
                <article-title>Reviewer response for version 1</article-title>
            </title-group>
            <contrib-group>
                <contrib contrib-type="author">
                    <name>
                        <surname>Subhi</surname>
                        <given-names>Mohammed</given-names>
                    </name>
                    <xref ref-type="aff" rid="r474768a1">1</xref>
                    <xref ref-type="aff" rid="r474768a2">2</xref>
                    <role>Referee</role>
                </contrib>
                <aff id="r474768a1">
                    <label>1</label>Directorate of Private University Education,, Baghdad, 10011,, Iraq</aff>
                <aff id="r474768a2">
                    <label>2</label>Department of Cybersecurity/ Directorate of Information Technology, Iraqi Ministry of Higher Education and Scientific Research, Baghdad, Baghdad, Iraq</aff>
            </contrib-group>
            <author-notes>
                <fn fn-type="conflict">
                    <p>
                        <bold>Competing interests: </bold>No competing interests were disclosed.</p>
                </fn>
            </author-notes>
            <pub-date pub-type="epub">
                <day>21</day>
                <month>4</month>
                <year>2026</year>
            </pub-date>
            <permissions>
                <copyright-statement>Copyright: &#x00a9; 2026 Subhi M</copyright-statement>
                <copyright-year>2026</copyright-year>
                <license xlink:href="https://creativecommons.org/licenses/by/4.0/">
                    <license-p>This is an open access peer review report distributed under the terms of the Creative Commons Attribution Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.</license-p>
                </license>
            </permissions>
            <related-article ext-link-type="doi" id="relatedArticleReport474768" related-article-type="peer-reviewed-article" xlink:href="10.12688/f1000research.173837.1"/>
            <custom-meta-group>
                <custom-meta>
                    <meta-name>recommendation</meta-name>
                    <meta-value>approve-with-reservations</meta-value>
                </custom-meta>
            </custom-meta-group>
        </front-stub>
        <body>
            <p>The manuscript presents an interesting and timely study on malware detection using RNA encoding combined with CNN architectures. The idea of integrating biologically inspired encoding with deep learning models is promising and shows potential for improving detection performance. However, several issues should be addressed to enhance the clarity, rigor, and overall quality of the manuscript.</p>
            <p> 1.&#x00a0;&#x00a0; &#x00a0;The abstract is generally well-structured; however, it would benefit from including additional details such as the dataset size and key aspects of the experimental setup to improve completeness.&#x00a0;</p>
            <p> 2.&#x00a0;&#x00a0; &#x00a0;The introduction provides a relevant background, but the manuscript requires careful language editing to correct grammatical issues and improve readability.&#x00a0;</p>
            <p> 3.&#x00a0;&#x00a0; &#x00a0;The related work section is comprehensive; however, it would be strengthened by adding a more critical analysis that clearly identifies the limitations of existing approaches and positions the proposed method accordingly.&#x00a0;</p>
            <p> 4.&#x00a0;&#x00a0; &#x00a0;The description of the CNN architecture is clear, but important training details such as optimizer type, learning rate, batch size, and number of epochs are missing and should be provided.&#x00a0;</p>
            <p> 5.&#x00a0;&#x00a0; &#x00a0;The discussion section would benefit from deeper insights into why RNA encoding enhances CNN performance, particularly from a feature representation perspective.&#x00a0;</p>
            <p> 6.&#x00a0;&#x00a0; &#x00a0;The conclusion is appropriate, but the future work section could be made more specific by outlining concrete and actionable research directions.</p>
            <p>Is the work clearly and accurately presented and does it cite the current literature?</p>
            <p>Partly</p>
            <p>If applicable, is the statistical analysis and its interpretation appropriate?</p>
            <p>Partly</p>
            <p>Are all the source data underlying the results available to ensure full reproducibility?</p>
            <p>No source data required</p>
            <p>Is the study design appropriate and is the work technically sound?</p>
            <p>Yes</p>
            <p>Are the conclusions drawn adequately supported by the results?</p>
            <p>Partly</p>
            <p>Are sufficient details of methods and analysis provided to allow replication by others?</p>
            <p>Partly</p>
            <p>Reviewer Expertise:</p>
            <p>Machine leaarning, artificial intelligience, Iot applications, and AI in security applications</p>
            <p>I confirm that I have read this submission and believe that I have an appropriate level of expertise to confirm that it is of an acceptable scientific standard, however I have significant reservations, as outlined above.</p>
        </body>
        <sub-article article-type="response" id="comment16006-474768">
            <front-stub>
                <contrib-group>
                    <contrib contrib-type="author">
                        <name>
                            <surname>Fitian Rashid</surname>
                            <given-names>Omar</given-names>
                        </name>
                    </contrib>
                </contrib-group>
                <author-notes>
                    <fn fn-type="conflict">
                        <p>
                            <bold>Competing interests: </bold>No competing interests were disclosed.</p>
                    </fn>
                </author-notes>
                <pub-date pub-type="epub">
                    <day>21</day>
                    <month>4</month>
                    <year>2026</year>
                </pub-date>
            </front-stub>
            <body>
                <p>
                    <bold>Reviewer Comments</bold>
                </p>
                <p> 
                    <bold>Comment 1:</bold>
                </p>
                <p> The abstract is generally well-structured; however, it would benefit from including additional details such as the dataset size and key aspects of the experimental setup to improve completeness.</p>
                <p> 
                    <bold>Response:</bold>
                </p>
                <p> Thank you for this valuable suggestion. The abstract has been revised to include the dataset size and key elements of the experimental setup, including the type of data used and evaluation protocol.</p>
                <p> </p>
                <p> 
                    <bold>Comment 2:</bold>
                </p>
                <p> The introduction provides a relevant background, but the manuscript requires careful language editing to correct grammatical issues and improve readability.</p>
                <p> 
                    <bold>Response:</bold>
                </p>
                <p> We appreciate this observation. The manuscript has undergone thorough language editing to improve grammar, sentence structure, and overall readability.</p>
                <p> </p>
                <p> 
                    <bold>Comment 3:</bold>
                </p>
                <p> The related work section is comprehensive; however, it would be strengthened by adding a more critical analysis that clearly identifies the limitations of existing approaches and positions the proposed method accordingly.</p>
                <p> 
                    <bold>Response:</bold>
                </p>
                <p> Thank you for this insightful comment. A critical analysis has been incorporated to highlight the limitations of existing methods and to clearly position the novelty and advantages of the proposed approach.</p>
                <p> </p>
                <p> 
                    <bold>Comment 4:</bold>
                </p>
                <p> The description of the CNN architecture is clear, but important training details such as optimizer type, learning rate, batch size, and number of epochs are missing and should be provided.</p>
                <p> 
                    <bold>Response:</bold>
                </p>
                <p> We appreciate this important suggestion. The training configuration details have been added to ensure reproducibility of the proposed method.</p>
                <p> 
                    <bold>Comment 5:</bold>
                </p>
                <p> The discussion section would benefit from deeper insights into why RNA encoding enhances CNN performance, particularly from a feature representation perspective.</p>
                <p> 
                    <bold>Response:</bold>
                </p>
                <p> Thank you for this constructive feedback. The discussion has been expanded to provide deeper insights into how RNA encoding improves feature representation and contributes to enhanced CNN performance.</p>
                <p> </p>
                <p> 
                    <bold>Comment 6:</bold>
                </p>
                <p> The conclusion is appropriate, but the future work section could be made more specific by outlining concrete and actionable research directions.</p>
                <p> 
                    <bold>Response:</bold>
                </p>
                <p> We appreciate this suggestion. The future work section has been revised to include specific and actionable research directions.</p>
            </body>
        </sub-article>
    </sub-article>
</article>
