<?xml version="1.0" encoding="UTF-8"?><!DOCTYPE article PUBLIC "-//NLM//DTD JATS (Z39.96) Journal Publishing DTD v1.2 20190208//EN" "http://jats.nlm.nih.gov/publishing/1.2/JATS-journalpublishing1.dtd"><article xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" article-type="research-article" dtd-version="1.2" xml:lang="en">
    <front>
        <journal-meta>
            <journal-id journal-id-type="pmc">F1000Research</journal-id>
            <journal-title-group>
                <journal-title>F1000Research</journal-title>
            </journal-title-group>
            <issn pub-type="epub">2046-1402</issn>
            <publisher>
                <publisher-name>F1000 Research Limited</publisher-name>
                <publisher-loc>London, UK</publisher-loc>
            </publisher>
        </journal-meta>
        <article-meta>
            <article-id pub-id-type="doi">10.12688/f1000research.174339.1</article-id>
            <article-categories>
                <subj-group subj-group-type="heading">
                    <subject>Research Article</subject>
                </subj-group>
                <subj-group>
                    <subject>Articles</subject>
                </subj-group>
            </article-categories>
            <title-group>
                <article-title>Insomnia, a sequel of stress among medical students in Iraq</article-title>
                <fn-group content-type="pub-status">
                    <fn>
                        <p>[version 1; peer review: awaiting peer review]</p>
                    </fn>
                </fn-group>
            </title-group>
            <contrib-group>
                <contrib contrib-type="author" corresp="yes">
                    <name>
                        <surname>Al Shawi</surname>
                        <given-names>Ameel F.</given-names>
                    </name>
                    <role content-type="http://credit.niso.org/">Data Curation</role>
                    <role content-type="http://credit.niso.org/">Formal Analysis</role>
                    <role content-type="http://credit.niso.org/">Funding Acquisition</role>
                    <uri content-type="orcid">https://orcid.org/0000-0003-3555-3492</uri>
                    <xref ref-type="corresp" rid="c1">a</xref>
                    <xref ref-type="aff" rid="a1">1</xref>
                    <xref ref-type="aff" rid="a2">2</xref>
                </contrib>
                <contrib contrib-type="author" corresp="no">
                    <name>
                        <surname>Tayib</surname>
                        <given-names>Zaid Mahmood</given-names>
                    </name>
                    <role content-type="http://credit.niso.org/">Data Curation</role>
                    <role content-type="http://credit.niso.org/">Formal Analysis</role>
                    <role content-type="http://credit.niso.org/">Writing &#x2013; Original Draft Preparation</role>
                    <xref ref-type="aff" rid="a1">1</xref>
                </contrib>
                <contrib contrib-type="author" corresp="no">
                    <name>
                        <surname>Lafta</surname>
                        <given-names>Riyadh K</given-names>
                    </name>
                    <role content-type="http://credit.niso.org/">Conceptualization</role>
                    <role content-type="http://credit.niso.org/">Data Curation</role>
                    <role content-type="http://credit.niso.org/">Software</role>
                    <role content-type="http://credit.niso.org/">Writing &#x2013; Review &amp; Editing</role>
                    <xref ref-type="aff" rid="a2">2</xref>
                </contrib>
                <contrib contrib-type="author" corresp="no">
                    <name>
                        <surname>Hasan</surname>
                        <given-names>Hasan Falah</given-names>
                    </name>
                    <role content-type="http://credit.niso.org/">Data Curation</role>
                    <role content-type="http://credit.niso.org/">Formal Analysis</role>
                    <role content-type="http://credit.niso.org/">Writing &#x2013; Original Draft Preparation</role>
                    <xref ref-type="aff" rid="a1">1</xref>
                </contrib>
                <contrib contrib-type="author" corresp="no">
                    <name>
                        <surname>Maamon Saaeed</surname>
                        <given-names>Muhannad</given-names>
                    </name>
                    <role content-type="http://credit.niso.org/">Data Curation</role>
                    <role content-type="http://credit.niso.org/">Formal Analysis</role>
                    <role content-type="http://credit.niso.org/">Writing &#x2013; Original Draft Preparation</role>
                    <xref ref-type="aff" rid="a1">1</xref>
                </contrib>
                <contrib contrib-type="author" corresp="no">
                    <name>
                        <surname>Noori</surname>
                        <given-names>Ahmed Sabah</given-names>
                    </name>
                    <role content-type="http://credit.niso.org/">Data Curation</role>
                    <role content-type="http://credit.niso.org/">Formal Analysis</role>
                    <role content-type="http://credit.niso.org/">Writing &#x2013; Original Draft Preparation</role>
                    <xref ref-type="aff" rid="a1">1</xref>
                </contrib>
                <aff id="a1">
                    <label>1</label>University of Fallujah, Fallujah, Iraq</aff>
                <aff id="a2">
                    <label>2</label>College of Medicine, Mustansiriyah University, Baghdad, Iraq</aff>
            </contrib-group>
            <author-notes>
                <corresp id="c1">
                    <label>a</label>
                    <email xlink:href="mailto:ameelalshawi@gmail.com">ameelalshawi@gmail.com</email>
                </corresp>
                <fn fn-type="conflict">
                    <p>No competing interests were disclosed.</p>
                </fn>
            </author-notes>
            <pub-date pub-type="epub">
                <day>14</day>
                <month>2</month>
                <year>2026</year>
            </pub-date>
            <pub-date pub-type="collection">
                <year>2026</year>
            </pub-date>
            <volume>15</volume>
            <elocation-id>258</elocation-id>
            <history>
                <date date-type="accepted">
                    <day>2</day>
                    <month>2</month>
                    <year>2026</year>
                </date>
            </history>
            <permissions>
                <copyright-statement>Copyright: &#x00a9; 2026 Al Shawi AF et al.</copyright-statement>
                <copyright-year>2026</copyright-year>
                <license xlink:href="https://creativecommons.org/licenses/by/4.0/">
                    <license-p>This is an open access article distributed under the terms of the Creative Commons Attribution Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.</license-p>
                </license>
            </permissions>
            <self-uri content-type="pdf" xlink:href="https://f1000research.com/articles/15-258/pdf"/>
            <abstract>
                <sec>
                    <title>Background</title>
                    <p>insomnia is common at any age; however, it is more prevalent among medical students due to the competitive environment they live. Stress is thought to be a primary contributor in the development of insomnia due to its two-way relationship with the disorder. The objective of this study is to assess sleep quality among medical students and explore the relation between insomnia and the level of stress they experience.</p>
                </sec>
                <sec>
                    <title>Methods</title>
                    <p>This cross-sectional study was conducted among medical students from Anbar and Fallujah universities in Anbar province/Iraq. All students who agreed to participate were included in the study. The presence of insomnia was evaluated according to the Athens insomnia scale (AIS-8). The level of stress was measured according to the perceived stress scale (PSS-10).</p>
                </sec>
                <sec>
                    <title>Results</title>
                    <p>The total number of participants was 452, 72.8% of whom reported insomnia. Low-stress level was experienced by 8.4%, moderate stress by 60.0%, and high perceived stress by 31.6% of the respondents. Level of stress was significantly associated with gender (p&lt; 0.001), with prevalence of insomnia among females (42.5%) been higher than that among males (30.3%). Logistic regression model revealed that students with high perceived stress had OR of 3.188 for insomnia compared to those with low level of stress (C.I. 2.1-4.8, P&lt;0.001).</p>
                </sec>
                <sec>
                    <title>Conclusion</title>
                    <p>Prevalence of insomnia among medical students is high, statistically attributed to stress. The findings of the study encourage adopting certain programs to enhance awareness of medical students about this problem.</p>
                </sec>
            </abstract>
            <kwd-group kwd-group-type="author">
                <kwd>Insomnia</kwd>
                <kwd>stress</kwd>
                <kwd>medical students</kwd>
                <kwd>Iraq</kwd>
            </kwd-group>
            <funding-group>
                <award-group id="fund-1">
                    <funding-source>None</funding-source>
                </award-group>
                <funding-statement>The author(s) declared that no grants were involved in supporting this work.</funding-statement>
            </funding-group>
        </article-meta>
    </front>
    <body>
        <sec id="sec5" sec-type="intro">
            <title>Introduction</title>
            <p>Insomnia is a common sleep disorder characterized by trouble falling asleep, staying asleep, or experiencing restful sleep, even when conditions are appropriate for sleep.
                <sup>
                    <xref ref-type="bibr" rid="ref1">1</xref>,
                    <xref ref-type="bibr" rid="ref2">2</xref>
                </sup> Multiple factors play a role in the development of insomnia, with stress being a primary contributor due to its two-way relationship with the disorder.
                <sup>
                    <xref ref-type="bibr" rid="ref3">3</xref>
                </sup> Other risk factors include demographic characteristics (such as being female, living alone, and old), genetic influences (like a family history of insomnia), respiratory issues (including asthma and sleep apnea), mental health conditions (such as anxiety and depression), and certain lifestyle habits (obesity, excessive caffeine intake, alcohol use, and smoking).
                <sup>
                    <xref ref-type="bibr" rid="ref4">4</xref>
                </sup>
            </p>
            <p>Due to the intense demands of clinical training and the extensive academic workload, medical students are especially vulnerable to high levels of stress, which can adversely affect their sleep quality and contribute to the onset of insomnia and other sleep disorders.
                <sup>
                    <xref ref-type="bibr" rid="ref5">5</xref>&#x2013;
                    <xref ref-type="bibr" rid="ref7">7</xref>
                </sup> Insomnia may negatively influence students' academic performance and overall health. Studies show that medical students experiencing insomnia often have lower Grade Point Averages than those without sleep difficulties.
                <sup>
                    <xref ref-type="bibr" rid="ref8">8</xref>,
                    <xref ref-type="bibr" rid="ref9">9</xref>
                </sup> Some students tend to use non-prescribed hypnotics or tranquilizers as a means of managing their sleep problems.
                <sup>
                    <xref ref-type="bibr" rid="ref10">10</xref>
                </sup>
            </p>
            <p>Sleep disturbances are reported more frequently among medical students compared to those in other academic fields.
                <sup>
                    <xref ref-type="bibr" rid="ref3">3</xref>
                </sup> Research has identified insomnia prevalence rates of 27.8% among medical students in China and 39.4% in India.
                <sup>
                    <xref ref-type="bibr" rid="ref11">11</xref>,
                    <xref ref-type="bibr" rid="ref12">12</xref>
                </sup> Likewise, insomnia is widely recognized as a common sleep disorder within the Arab region.
                <sup>
                    <xref ref-type="bibr" rid="ref13">13</xref>&#x2013;
                    <xref ref-type="bibr" rid="ref15">15</xref>
                </sup>
            </p>
            <p>In Iraq, medical students have faced unique and ongoing challenges, such as political instability, economic difficulties, and security issues, which contributed to the high prevalence of insomnia within this group.
                <sup>
                    <xref ref-type="bibr" rid="ref16">16</xref>,
                    <xref ref-type="bibr" rid="ref17">17</xref>
                </sup>
            </p>
            <p>This survey aims to assess the prevalence of insomnia among a sample of medical students, and to highlight its relation to stress as a potential predictor.</p>
        </sec>
        <sec id="sec6" sec-type="methods">
            <title>Methods</title>
            <sec id="sec7">
                <title>Design and study population</title>
                <p>This cross-sectional study was conducted during the academic year 2022&#x2013;2023, and involved students from medical colleges at Anbar and Fallujah Universities, that are located in Anbar Province (which is located to the west of Baghdad, the capital of Iraq). All students from the six academic years, who agreed to participate after being informed about the purpose of the survey, were included in the study via a convenience sampling technique.</p>
            </sec>
            <sec id="sec8">
                <title>Study instruments</title>
                <p>The self-administered questionnaire comprised three main sections. The first section collected socio-demographic information, including age, gender, education level, place of residence, family income, smoking status, medical history, and family history of insomnia.</p>
                <p>The second section included questions borrowed from the Athens Insomnia Scale (AIS-8), which is a self-assessment psychometric instrument that has previously been adopted, has shown a high consistency, reliability, and an external validity for the evaluation of the intensity of sleep difficulty, and can be utilized in clinical practice and research as an instrument to measure the intensity of sleep-related problems, and also as a screening tool in establishing the diagnosis of insomnia.
                    <sup>
                        <xref ref-type="bibr" rid="ref18">18</xref>&#x2013;
                        <xref ref-type="bibr" rid="ref20">20</xref>
                    </sup> It consists of 8-items; the first five investigate sleep procedure (sleep induction, night awakening, awakening in the early morning, total sleep duration, and quality of sleep), while the last three refer to well-being, functioning capacity, and sleepiness during the day. The 8 items are scored on a 4-point rating scale ranging from 0 to 3, with 0 refers to &#x2018;no problem&#x2019; with the corresponding sleep parameter and 3 refers to a serious problem (occurrence of more than three times a week in the past month). A total AIS score of &#x2265;6 indicates insomnia.</p>
                <p>The third section was the Perceived Stress Scale 10 (PSS-10), which is a global measure of perceived stress that proved reliable and valid.
                    <sup>
                        <xref ref-type="bibr" rid="ref21">21</xref>
                    </sup> The ten items are scored on a 5-point rating scale ranging from 0 (never) to 4 (very often). A total score ranging from 0 to 40 is calculated by reverse scoring the four positively worded items and then summing all the items on the scale. The questions assess feelings and thoughts experienced over the past month. Higher total scores reflect greater levels of psychological distress or stress, while lower scores indicate better coping abilities and emotional well-being. Subscale scores were computed by summing the six negatively worded items (1, 2, 3, 6, 9, and 10) and the four positively worded items (4, 5, 7, and 8). Stress levels were classified into the following 3 categories: low stress (PSS score &#x2264;13), moderate stress (PSS score 14-26), and high stress (PSS score &#x2265;27).</p>
            </sec>
            <sec id="sec9">
                <title>Data collection</title>
                <p>Data collection was carried out during March 20, 2023 for four weeks.</p>
                <p>The questionnaire was administered in Arabic language and filled through a face-to-face interview that took about ten minutes for each student. The process of data collection was performed during March through April 2023.</p>
            </sec>
            <sec id="sec10">
                <title>Ethics</title>
                <p>The research was approved by the Scientific Committee at the Medical College/University of Fallujah. A verbal consent was obtained from each of the participants prior to the interview as written consent is very difficult to be taken from the participants in our culture, for it is considered as a sensitive procedure that might raise suspicion, and is only done when there is a serious surgical intervention. The respondents were informed that the information they give would be kept confidential and anonymous, accessed by no one but the authorized personnel, and would not be used for other than research purposes.</p>
            </sec>
            <sec id="sec11">
                <title>Analyses</title>
                <p>Data entry was done by using the Microsoft Excel software program. The statistical analysis was performed using SPSS software version 26. The characteristics of the sample were summarized using means and standard deviations for continuous variables, and frequencies/percentages for categorical variables. Chi-squared test was used to measure the association between insomnia and stress with other variables, while adjusted logistic regression test was applied to measure the odds ratio and to exclude the effect of other confounders such as age and gender. P value of 0.05 was considered as a cut-off level for significance.</p>
            </sec>
        </sec>
        <sec id="sec12" sec-type="results">
            <title>Results</title>
            <p>The total number of participants was 452 medical students, with a mean age of 21.43&#x00b1;1.912 years (range&#x2009;=&#x2009;17&#x2013;27 years). Female students (259) formed 57.3%, while males (193) formed 42.7%% of the sample. Students in the preclinical stage (first three years of medical study) formed 52.7%, while those in the clinical stages (4
                <sup>th</sup>, 5
                <sup>th</sup>, and 6
                <sup>th</sup> years) formed 47.3% of the sample, as showed in 
                <xref ref-type="table" rid="T1">Table 1</xref>, which also demonstrates that 72.8% of the participants reported having insomnia, while 31.6% had high perceived stress level.</p>
            <table-wrap id="T1" orientation="portrait" position="float">
                <label>
Table 1. </label>
                <caption>
                    <title>Sociodemographic characteristics of the sample (n = 452).</title>
                </caption>
                <table content-type="article-table" frame="hsides">
                    <thead>
                        <tr>
                            <th align="left" colspan="1" rowspan="1" valign="top">Characteristics</th>
                            <th align="left" colspan="1" rowspan="1" valign="top"/>
                            <th align="left" colspan="1" rowspan="1" valign="top">n</th>
                            <th align="left" colspan="1" rowspan="1" valign="top">
%</th>
                        </tr>
                    </thead>
                    <tbody>
                        <tr>
                            <td align="left" colspan="1" rowspan="2" valign="middle">Gender</td>
                            <td align="left" colspan="1" rowspan="1" valign="middle">Male</td>
                            <td align="left" colspan="1" rowspan="1" valign="middle">193</td>
                            <td align="left" colspan="1" rowspan="1" valign="middle">42.7</td>
                        </tr>
                        <tr>
                            <td align="left" colspan="1" rowspan="1" valign="middle">Female</td>
                            <td align="left" colspan="1" rowspan="1" valign="middle">259</td>
                            <td align="left" colspan="1" rowspan="1" valign="middle">57.3</td>
                        </tr>
                        <tr>
                            <td align="left" colspan="1" rowspan="6" valign="middle">Academic year</td>
                            <td align="left" colspan="1" rowspan="1" valign="middle">1
                                <sup>st</sup> stage</td>
                            <td align="left" colspan="1" rowspan="1" valign="middle">66</td>
                            <td align="left" colspan="1" rowspan="1" valign="middle">14.6</td>
                        </tr>
                        <tr>
                            <td align="left" colspan="1" rowspan="1" valign="middle">2
                                <sup>nd</sup> stage</td>
                            <td align="left" colspan="1" rowspan="1" valign="middle">57</td>
                            <td align="left" colspan="1" rowspan="1" valign="middle">12.6</td>
                        </tr>
                        <tr>
                            <td align="left" colspan="1" rowspan="1" valign="middle">3
                                <sup>rd</sup> stage</td>
                            <td align="left" colspan="1" rowspan="1" valign="middle">115</td>
                            <td align="left" colspan="1" rowspan="1" valign="middle">25.4</td>
                        </tr>
                        <tr>
                            <td align="left" colspan="1" rowspan="1" valign="middle">4
                                <sup>th</sup> stage</td>
                            <td align="left" colspan="1" rowspan="1" valign="middle">126</td>
                            <td align="left" colspan="1" rowspan="1" valign="middle">27.9</td>
                        </tr>
                        <tr>
                            <td align="left" colspan="1" rowspan="1" valign="middle">5
                                <sup>th</sup> stage</td>
                            <td align="left" colspan="1" rowspan="1" valign="middle">58</td>
                            <td align="left" colspan="1" rowspan="1" valign="middle">12.8</td>
                        </tr>
                        <tr>
                            <td align="left" colspan="1" rowspan="1" valign="middle">6
                                <sup>th</sup> stage</td>
                            <td align="left" colspan="1" rowspan="1" valign="middle">30</td>
                            <td align="left" colspan="1" rowspan="1" valign="middle">6.6</td>
                        </tr>
                        <tr>
                            <td align="left" colspan="1" rowspan="2" valign="middle">Smoking history</td>
                            <td align="left" colspan="1" rowspan="1" valign="middle">Yes</td>
                            <td align="left" colspan="1" rowspan="1" valign="middle">30</td>
                            <td align="left" colspan="1" rowspan="1" valign="middle">8.6</td>
                        </tr>
                        <tr>
                            <td align="left" colspan="1" rowspan="1" valign="middle">No</td>
                            <td align="left" colspan="1" rowspan="1" valign="middle">413</td>
                            <td align="left" colspan="1" rowspan="1" valign="middle">91.4</td>
                        </tr>
                        <tr>
                            <td align="left" colspan="1" rowspan="2" valign="middle">Insomnia</td>
                            <td align="left" colspan="1" rowspan="1" valign="middle">Yes</td>
                            <td align="left" colspan="1" rowspan="1" valign="middle">329</td>
                            <td align="left" colspan="1" rowspan="1" valign="middle">72.8</td>
                        </tr>
                        <tr>
                            <td align="left" colspan="1" rowspan="1" valign="middle">No</td>
                            <td align="left" colspan="1" rowspan="1" valign="middle">123</td>
                            <td align="left" colspan="1" rowspan="1" valign="middle">27.2</td>
                        </tr>
                        <tr>
                            <td align="left" colspan="1" rowspan="3" valign="middle">Stress</td>
                            <td align="left" colspan="1" rowspan="1" valign="middle">Low stress</td>
                            <td align="left" colspan="1" rowspan="1" valign="middle">38</td>
                            <td align="left" colspan="1" rowspan="1" valign="middle">8.4</td>
                        </tr>
                        <tr>
                            <td align="left" colspan="1" rowspan="1" valign="middle">Moderate stress</td>
                            <td align="left" colspan="1" rowspan="1" valign="middle">271</td>
                            <td align="left" colspan="1" rowspan="1" valign="middle">60</td>
                        </tr>
                        <tr>
                            <td align="left" colspan="1" rowspan="1" valign="middle">High perceived stress</td>
                            <td align="left" colspan="1" rowspan="1" valign="middle">143</td>
                            <td align="left" colspan="1" rowspan="1" valign="middle">31.6</td>
                        </tr>
                    </tbody>
                </table>
            </table-wrap>
            <p>
                <xref ref-type="table" rid="T2">
Table 2</xref> shows a statistically significant relation between level of stress and insomnia (p &lt; 0.001). Level of stress was significantly associated with gender (p &lt; 0.001). The prevalence of insomnia among females was 42.5%, which is higher than that among males (30.3%), as demonstrated in 
                <xref ref-type="fig" rid="f1">Figure 1</xref>. However, there was no statistically significant association between insomnia and gender (p = 0.457). The results indicated that the prevalence of insomnia among students in the pre-clinical stages was 76.5%, while it was 69% among students in the clinical stages. Statistical analysis showed no significant association between insomnia and academic year (p = 0.363), or between stress and academic year (p = 0.448) (
                <xref ref-type="table" rid="T3">
Table 3</xref>).</p>
            <table-wrap id="T2" orientation="portrait" position="float">
                <label>
Table 2. </label>
                <caption>
                    <title>Relation between level of stress and insomnia among medical students.</title>
                </caption>
                <table content-type="article-table" frame="hsides">
                    <thead>
                        <tr>
                            <th align="left" colspan="1" rowspan="1" valign="top"/>
                            <th align="left" colspan="1" rowspan="1" valign="top">No insomnia n(%)</th>
                            <th align="left" colspan="1" rowspan="1" valign="top">Insomnia n(%)</th>
                            <th align="left" colspan="1" rowspan="1" valign="top">Total n(%)</th>
                            <th align="left" colspan="1" rowspan="1" valign="top">
</th>
                        </tr>
                    </thead>
                    <tbody>
                        <tr>
                            <td align="left" colspan="1" rowspan="1" valign="top">Low stress</td>
                            <td align="left" colspan="1" rowspan="1" valign="top">24 (5.3%)</td>
                            <td align="left" colspan="1" rowspan="1" valign="top">14 (3.1%)</td>
                            <td align="left" colspan="1" rowspan="1" valign="top">38 (8.4%)</td>
                            <td align="left" colspan="1" rowspan="1" valign="top">p &lt; 0.001</td>
                        </tr>
                        <tr>
                            <td align="left" colspan="1" rowspan="1" valign="top">Moderate stress</td>
                            <td align="left" colspan="1" rowspan="1" valign="top">78 (17.3%)</td>
                            <td align="left" colspan="1" rowspan="1" valign="top">193 (42.7%)</td>
                            <td align="left" colspan="1" rowspan="1" valign="top">271 (60.0%)</td>
                            <td colspan="1" rowspan="1"/>
                        </tr>
                        <tr>
                            <td align="left" colspan="1" rowspan="1" valign="top">High perceived stress</td>
                            <td align="left" colspan="1" rowspan="1" valign="top">21 (4.6%)</td>
                            <td align="left" colspan="1" rowspan="1" valign="top">122 (27.0%)</td>
                            <td align="left" colspan="1" rowspan="1" valign="top">143 (31.6%)</td>
                            <td colspan="1" rowspan="1"/>
                        </tr>
                        <tr>
                            <td align="left" colspan="1" rowspan="1" valign="top">TOTAL</td>
                            <td align="left" colspan="1" rowspan="1" valign="top">23 (27.2%)</td>
                            <td align="left" colspan="1" rowspan="1" valign="top">329 (72.8%)</td>
                            <td align="left" colspan="1" rowspan="1" valign="top">452 (100.0%)</td>
                            <td colspan="1" rowspan="1"/>
                        </tr>
                    </tbody>
                </table>
            </table-wrap>
            <fig fig-type="figure" id="f1" orientation="portrait" position="float">
                <label>
Figure 1. </label>
                <caption>
                    <title>Distribution of insomnia by gender.</title>
                </caption>
                <graphic id="gr1" orientation="portrait" position="float" xlink:href="https://f1000research-files.f1000.com/manuscripts/192230/9f65586e-33e0-405e-8d21-ecc217b26d91_figure1.gif"/>
            </fig>
            <table-wrap id="T3" orientation="portrait" position="float">
                <label>
Table 3. </label>
                <caption>
                    <title>Crosstabulation of insomnia, stress, gender, and academic year.</title>
                </caption>
                <table content-type="article-table" frame="hsides">
                    <thead>
                        <tr>
                            <th align="left" colspan="2" rowspan="3" valign="top"/>
                            <th align="left" colspan="3" rowspan="1" valign="top">Level of stress</th>
                            <th align="left" colspan="1" rowspan="1" valign="top"/>
                            <th align="left" colspan="2" rowspan="1" valign="top">Insomnia</th>
                            <th align="left" colspan="1" rowspan="1" valign="top"/>
                        </tr>
                        <tr>
                            <th align="left" colspan="1" rowspan="1" valign="top">
Low</th>
                            <th align="left" colspan="1" rowspan="1" valign="top">Moderate</th>
                            <th align="left" colspan="1" rowspan="1" valign="top">High</th>
                            <th align="left" colspan="1" rowspan="1" valign="top">

                                <italic toggle="yes">P*</italic>
</th>
                            <th align="left" colspan="1" rowspan="1" valign="top">Yes</th>
                            <th align="left" colspan="1" rowspan="1" valign="top">No</th>
                            <th align="left" colspan="1" rowspan="1" valign="top">

                                <italic toggle="yes">P*</italic>
</th>
                        </tr>
                        <tr>
                            <th align="left" colspan="1" rowspan="1" valign="top">n (%)</th>
                            <th align="left" colspan="1" rowspan="1" valign="top">
n (%)</th>
                            <th align="left" colspan="1" rowspan="1" valign="top">
n (%)</th>
                            <th colspan="1" rowspan="1"/>
                            <th align="left" colspan="1" rowspan="1" valign="top">n (%)</th>
                            <th align="left" colspan="1" rowspan="1" valign="top">n (%)</th>
                            <th colspan="1" rowspan="1"/>
                        </tr>
                    </thead>
                    <tbody>
                        <tr>
                            <td align="left" colspan="1" rowspan="2" valign="top">Gender</td>
                            <td align="left" colspan="1" rowspan="1" valign="top">Male</td>
                            <td align="left" colspan="1" rowspan="1" valign="top">24 (5.3%)</td>
                            <td align="left" colspan="1" rowspan="1" valign="top">133 (29.4%)</td>
                            <td align="left" colspan="1" rowspan="1" valign="top">36 (8.0%)</td>
                            <td align="left" colspan="1" rowspan="1" valign="top">
&lt; 0.001</td>
                            <td align="left" colspan="1" rowspan="1" valign="top">
137 (30.3%)</td>
                            <td align="left" colspan="1" rowspan="1" valign="top">
56 (12.4%)</td>
                            <td align="left" colspan="1" rowspan="1" valign="top">0.457</td>
                        </tr>
                        <tr>
                            <td align="left" colspan="1" rowspan="1" valign="top">Female</td>
                            <td align="left" colspan="1" rowspan="1" valign="top">14 (3.1%)</td>
                            <td align="left" colspan="1" rowspan="1" valign="top">138 (30.5%)</td>
                            <td align="left" colspan="1" rowspan="1" valign="top">107 (23.7%)</td>
                            <td colspan="1" rowspan="1"/>
                            <td align="left" colspan="1" rowspan="1" valign="top">192 (42.5%)</td>
                            <td align="left" colspan="1" rowspan="1" valign="top">67 (14.8%)</td>
                            <td colspan="1" rowspan="1"/>
                        </tr>
                        <tr>
                            <td align="left" colspan="1" rowspan="6" valign="top">Academic year</td>
                            <td align="left" colspan="1" rowspan="1" valign="top">1
                                <sup>st</sup>
                            </td>
                            <td align="left" colspan="1" rowspan="1" valign="top">8 (1.8%)</td>
                            <td align="left" colspan="1" rowspan="1" valign="top">39 (8.6%)</td>
                            <td align="left" colspan="1" rowspan="1" valign="top">19 (4.2%)</td>
                            <td align="left" colspan="1" rowspan="1" valign="top">0.363</td>
                            <td align="left" colspan="1" rowspan="1" valign="top">51 (11.3%)</td>
                            <td align="left" colspan="1" rowspan="1" valign="top">15 (3.3%)</td>
                            <td colspan="1" rowspan="1"/>
                        </tr>
                        <tr>
                            <td align="left" colspan="1" rowspan="1" valign="top">2
                                <sup>nd</sup>
                            </td>
                            <td align="left" colspan="1" rowspan="1" valign="top">7 (1.5%)</td>
                            <td align="left" colspan="1" rowspan="1" valign="top">31 (6.9%)</td>
                            <td align="left" colspan="1" rowspan="1" valign="top">19 (4.2%)</td>
                            <td colspan="1" rowspan="1"/>
                            <td align="left" colspan="1" rowspan="1" valign="top">44 (9.7%)</td>
                            <td align="left" colspan="1" rowspan="1" valign="top">13 (2.9%)</td>
                            <td colspan="1" rowspan="1"/>
                        </tr>
                        <tr>
                            <td align="left" colspan="1" rowspan="1" valign="top">3
                                <sup>rd</sup>
                            </td>
                            <td align="left" colspan="1" rowspan="1" valign="top">8 (1.8%)</td>
                            <td align="left" colspan="1" rowspan="1" valign="top">68 (15.0%)</td>
                            <td align="left" colspan="1" rowspan="1" valign="top">39 (8.6%)</td>
                            <td colspan="1" rowspan="1"/>
                            <td align="left" colspan="1" rowspan="1" valign="top">87 (19.2%)</td>
                            <td align="left" colspan="1" rowspan="1" valign="top">28 (6.2%)</td>
                            <td colspan="1" rowspan="1"/>
                        </tr>
                        <tr>
                            <td align="left" colspan="1" rowspan="1" valign="top">4
                                <sup>th</sup>
                            </td>
                            <td align="left" colspan="1" rowspan="1" valign="top">8 (1.8%)</td>
                            <td align="left" colspan="1" rowspan="1" valign="top">74 (16.4%)</td>
                            <td align="left" colspan="1" rowspan="1" valign="top">44 (9.7%)</td>
                            <td colspan="1" rowspan="1"/>
                            <td align="left" colspan="1" rowspan="1" valign="top">84 (18.6%)</td>
                            <td align="left" colspan="1" rowspan="1" valign="top">42 (9.3%)</td>
                            <td colspan="1" rowspan="1"/>
                        </tr>
                        <tr>
                            <td align="left" colspan="1" rowspan="1" valign="top">5
                                <sup>th</sup>
                            </td>
                            <td align="left" colspan="1" rowspan="1" valign="top">4 (0.9%)</td>
                            <td align="left" colspan="1" rowspan="1" valign="top">35 (7.7%)</td>
                            <td align="left" colspan="1" rowspan="1" valign="top">19 (4.2%)</td>
                            <td colspan="1" rowspan="1"/>
                            <td align="left" colspan="1" rowspan="1" valign="top">40 (8.8%)</td>
                            <td align="left" colspan="1" rowspan="1" valign="top">18 (4.0%)</td>
                            <td colspan="1" rowspan="1"/>
                        </tr>
                        <tr>
                            <td align="left" colspan="1" rowspan="1" valign="top">6
                                <sup>th</sup>
                            </td>
                            <td align="left" colspan="1" rowspan="1" valign="top">3 (0.7%)</td>
                            <td align="left" colspan="1" rowspan="1" valign="top">24 (5.3%)</td>
                            <td align="left" colspan="1" rowspan="1" valign="top">3 (0.7%)</td>
                            <td colspan="1" rowspan="1"/>
                            <td align="left" colspan="1" rowspan="1" valign="top">23 (5.1%)</td>
                            <td align="left" colspan="1" rowspan="1" valign="top">7 (1.5%)</td>
                            <td colspan="1" rowspan="1"/>
                        </tr>
                    </tbody>
                </table>
            </table-wrap>
            <p>Logistic regression model revealed that students with high perceived stress had OR of 3.188 for insomnia compared to those with low level of stress (C.I. 2.1-4.8, P &lt; 0.001). The association of insomnia with gender or age was insignificant (
                <xref ref-type="table" rid="T4">
Table 4</xref>).</p>
            <table-wrap id="T4" orientation="portrait" position="float">
                <label>
Table 4. </label>
                <caption>
                    <title>Adjusted logistic regression for insomnia and dependent variables (stress, gender, age, and academic year).</title>
                </caption>
                <table content-type="article-table" frame="hsides">
                    <thead>
                        <tr>
                            <th align="left" colspan="2" rowspan="2" valign="top"/>
                            <th align="left" colspan="1" rowspan="2" valign="top">B</th>
                            <th align="left" colspan="1" rowspan="2" valign="top">OR</th>
                            <th align="left" colspan="2" rowspan="1" valign="top">95% C.I. for EXP(B)</th>
                            <th align="left" colspan="1" rowspan="2" valign="top">P</th>
                        </tr>
                        <tr>
                            <th align="left" colspan="1" rowspan="1" valign="top">Lower</th>
                            <th align="left" colspan="1" rowspan="1" valign="top">
Upper</th>
                        </tr>
                    </thead>
                    <tbody>
                        <tr>
                            <td align="left" colspan="1" rowspan="5" valign="top">Step 1
                                <sup>a</sup>
                            </td>
                            <td align="left" colspan="1" rowspan="1" valign="top">Level of stress</td>
                            <td align="left" colspan="1" rowspan="1" valign="top">1.148</td>
                            <td align="left" colspan="1" rowspan="1" valign="top">3.151</td>
                            <td align="left" colspan="1" rowspan="1" valign="top">2.095</td>
                            <td align="left" colspan="1" rowspan="1" valign="top">4.738</td>
                            <td align="left" colspan="1" rowspan="1" valign="top">&lt;0.001</td>
                        </tr>
                        <tr>
                            <td align="left" colspan="1" rowspan="1" valign="top">Gender</td>
                            <td align="left" colspan="1" rowspan="1" valign="top">-0.178</td>
                            <td align="left" colspan="1" rowspan="1" valign="top">0.837</td>
                            <td align="left" colspan="1" rowspan="1" valign="top">0.534</td>
                            <td align="left" colspan="1" rowspan="1" valign="top">1.312</td>
                            <td align="left" colspan="1" rowspan="1" valign="top">0.438</td>
                        </tr>
                        <tr>
                            <td align="left" colspan="1" rowspan="1" valign="top">Age</td>
                            <td align="left" colspan="1" rowspan="1" valign="top">-0.147</td>
                            <td align="left" colspan="1" rowspan="1" valign="top">0.863</td>
                            <td align="left" colspan="1" rowspan="1" valign="top">0.719</td>
                            <td align="left" colspan="1" rowspan="1" valign="top">1.036</td>
                            <td align="left" colspan="1" rowspan="1" valign="top">0.114</td>
                        </tr>
                        <tr>
                            <td align="left" colspan="1" rowspan="1" valign="top">Academic year</td>
                            <td align="left" colspan="1" rowspan="1" valign="top">0.052</td>
                            <td align="left" colspan="1" rowspan="1" valign="top">1.053</td>
                            <td align="left" colspan="1" rowspan="1" valign="top">0.826</td>
                            <td align="left" colspan="1" rowspan="1" valign="top">1.344</td>
                            <td align="left" colspan="1" rowspan="1" valign="top">0.675</td>
                        </tr>
                        <tr>
                            <td align="left" colspan="1" rowspan="1" valign="top">Constant</td>
                            <td align="left" colspan="1" rowspan="1" valign="top">1.779</td>
                            <td align="left" colspan="1" rowspan="1" valign="top">5.922</td>
                            <td colspan="1" rowspan="1"/>
                            <td colspan="1" rowspan="1"/>
                            <td align="left" colspan="1" rowspan="1" valign="top">0.314</td>
                        </tr>
                    </tbody>
                </table>
            </table-wrap>
        </sec>
        <sec id="sec13" sec-type="discussion">
            <title>Discussion</title>
            <p>Medical students frequently face high levels of stress due to long study hours, frequent exams, and the intense demands of clinical training. This persistent stress can lead to insomnia, making it difficult to fall asleep or maintain restful sleep.
                <sup>
                    <xref ref-type="bibr" rid="ref6">6</xref>,
                    <xref ref-type="bibr" rid="ref22">22</xref>
                </sup> The present study tried to explore the relationship between stress and insomnia, driven by the observation that many medical students engage in poor sleep habits, such as inconsistent sleep schedules and insufficient sleep duration. These sleep difficulties&#x2014;along with symptoms like fatigue, reduced cognitive performance, and mood disturbances&#x2014;can significantly affect their academic success, overall well-being, and quality of life.</p>
            <p>The findings of this study indicate a significantly high prevalence of insomnia among medical students, consistent with findings from previous studies across the region. Reported rates of poor sleep quality include 76% in Saudi Arabia, 55.7% in Egypt, 70.2% in Jordan, and 37.1% in Lebanon.
                <sup>
                    <xref ref-type="bibr" rid="ref23">23</xref>&#x2013;
                    <xref ref-type="bibr" rid="ref25">25</xref>
                </sup>
            </p>
            <p>In the Iraqi context, several factors likely contribute to elevated stress and insomnia rates, including ongoing security instability, exposure to violence, economic challenges, political uncertainty, overcrowding, difficult living conditions, social tensions, and limited access to mental health support.
                <sup>
                    <xref ref-type="bibr" rid="ref26">26</xref>
                </sup>
            </p>
            <p>This study also revealed a high overall level of stress among medical students, consistent with earlier findings in Iraq, where 30.2% reported experiencing high perceived stress.
                <sup>
                    <xref ref-type="bibr" rid="ref27">27</xref>
                </sup> Comparable levels were observed in other studies&#x2014;using various stress assessment tools&#x2014;in both developed and developing nations, including 30.9% in Egypt, 28.9% in Saudi Arabia, and 49.9% in Singapore.
                <sup>
                    <xref ref-type="bibr" rid="ref28">28</xref>
                </sup>
            </p>
            <p>Insomnia was found to be more common among females than males. Multiple studies have indicated that female medical students are more likely to experience higher levels of insomnia and stress compared to their male counterparts.
                <sup>
                    <xref ref-type="bibr" rid="ref29">29</xref>,
                    <xref ref-type="bibr" rid="ref30">30</xref>
                </sup>
            </p>
            <p>Students with elevated stress levels showed a greater occurrence of insomnia than those with lower stress levels. Although stress was significantly associated with gender in favor of females, the association between gender and insomnia was not significant, probably because females are more resilient than males due to biological and cultural factors.
                <sup>
                    <xref ref-type="bibr" rid="ref31">31</xref>
                </sup> Logistic regression analysis reinforced this result, revealing that stress significantly increases the risk of insomnia, thereby confirming the hypothesis that stress is a key contributing factor to the development of insomnia.</p>
            <p>Additionally, the study observed a higher prevalence of insomnia among students in the preclinical phase compared to those undergoing clinical training, although no significant link was found between insomnia and academic year. This pattern might be attributed to that clinical-stage students are adapting better to academic demands and managing their sleep schedules more effectively than preclinical students. Interestingly, these findings differ from a study conducted in Saudi Arabia, which reported a higher rate of insomnia among medical students in clinical stages compared to those in preclinical stages.
                <sup>
                    <xref ref-type="bibr" rid="ref32">32</xref>
                </sup>
            </p>
            <sec id="sec14">
                <title>Limitations</title>
                <p>The current study adopted a cross-sectional design, which lacks the temporal relationship between factor and outcome. All variables were assessed at the same time, limiting the ability to draw causal inferences. Besides, we could not exclude the role of confounders that may influence the relation between stress and insomnia. Lastly, stress and insomnia were measured using a self-report questionnaire, which can be affected by recall bias, people may over report or underreport certain conditions due to lack of insight, social desirability concerns or poor recall.</p>
            </sec>
        </sec>
        <sec id="sec15" sec-type="conclusion">
            <title>Conclusion</title>
            <p>The prevalence of insomnia among medical students is high, with a significant association with stress, suggesting that stress is an important factor contributing to insomnia among medical students. It is important to raise the awareness of medical students about how stress exacerbates sleep problems and reflects on general health and wellbeing through short education programs that helps build their resilience.</p>
        </sec>
        <sec id="sec16">
            <title>Ethics statement</title>
            <p>This is an observational study that did not involve any human intervention. The study was approved by the Ethical and Scientific Committee for research in College of Medicine/University of Fallujah, under approval SC NO. 2022/3 UOF.</p>
        </sec>
    </body>
    <back>
        <sec id="sec19" sec-type="data-availability">
            <title>Data availability</title>
            <p>The data that support the findings of this study are openly available in figshare; 
                <ext-link ext-link-type="uri" xlink:href="https://doi.org/10.6084/m9.figshare.31127239.v1">https://doi.org/10.6084/m9.figshare.31127239.v1</ext-link>.
                <sup>
                    <xref ref-type="bibr" rid="ref33">33</xref>
                </sup>
            </p>
        </sec>
        <ack>
            <title>Acknowledgements</title>
            <p>We are grateful to all the people who participated in the study. With special thanks for Othman Talib Alwan, Suhaib Majlis for fascilasting the data collection.</p>
        </ack>
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