<?xml version="1.0" encoding="UTF-8"?><!DOCTYPE article PUBLIC "-//NLM//DTD JATS (Z39.96) Journal Publishing DTD v1.2 20190208//EN" "http://jats.nlm.nih.gov/publishing/1.2/JATS-journalpublishing1.dtd"><article xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" article-type="other" dtd-version="1.2" xml:lang="en">
    <front>
        <journal-meta>
            <journal-id journal-id-type="pmc">F1000Research</journal-id>
            <journal-title-group>
                <journal-title>F1000Research</journal-title>
            </journal-title-group>
            <issn pub-type="epub">2046-1402</issn>
            <publisher>
                <publisher-name>F1000 Research Limited</publisher-name>
                <publisher-loc>London, UK</publisher-loc>
            </publisher>
        </journal-meta>
        <article-meta>
            <article-id pub-id-type="doi">10.12688/f1000research.179720.1</article-id>
            <article-categories>
                <subj-group subj-group-type="heading">
                    <subject>Study Protocol</subject>
                </subj-group>
                <subj-group>
                    <subject>Articles</subject>
                </subj-group>
            </article-categories>
            <title-group>
                <article-title>
                    <italic>Protocol for a study on social belonging wise intervention to address adverse childhood experiences and improve Kenyan high school students' academic performance and general well-being</italic>
                </article-title>
                <fn-group content-type="pub-status">
                    <fn>
                        <p>[version 1; peer review: awaiting peer review]</p>
                    </fn>
                </fn-group>
            </title-group>
            <contrib-group>
                <contrib contrib-type="author" corresp="yes">
                    <name>
                        <surname>C Mwendwa</surname>
                        <given-names>Anne</given-names>
                    </name>
                    <role content-type="http://credit.niso.org/">Conceptualization</role>
                    <role content-type="http://credit.niso.org/">Methodology</role>
                    <role content-type="http://credit.niso.org/">Writing &#x2013; Original Draft Preparation</role>
                    <role content-type="http://credit.niso.org/">Writing &#x2013; Review &amp; Editing</role>
                    <uri content-type="orcid">https://orcid.org/0009-0002-4488-6862</uri>
                    <xref ref-type="corresp" rid="c1">a</xref>
                    <xref ref-type="aff" rid="a1">1</xref>
                </contrib>
                <contrib contrib-type="author" corresp="no">
                    <name>
                        <surname>Obondo</surname>
                        <given-names>Anne</given-names>
                    </name>
                    <role content-type="http://credit.niso.org/">Supervision</role>
                    <role content-type="http://credit.niso.org/">Validation</role>
                    <xref ref-type="aff" rid="a2">2</xref>
                </contrib>
                <contrib contrib-type="author" corresp="no">
                    <name>
                        <surname>Mutavi</surname>
                        <given-names>Teresia</given-names>
                    </name>
                    <role content-type="http://credit.niso.org/">Supervision</role>
                    <role content-type="http://credit.niso.org/">Validation</role>
                    <uri content-type="orcid">https://orcid.org/0000-0002-2585-6556</uri>
                    <xref ref-type="aff" rid="a3">3</xref>
                </contrib>
                <contrib contrib-type="author" corresp="no">
                    <name>
                        <surname>Kumar</surname>
                        <given-names>Manasi</given-names>
                    </name>
                    <role content-type="http://credit.niso.org/">Supervision</role>
                    <role content-type="http://credit.niso.org/">Validation</role>
                    <xref ref-type="aff" rid="a4">4</xref>
                    <xref ref-type="aff" rid="a5">5</xref>
                </contrib>
                <aff id="a1">
                    <label>1</label>Department of Psychiatry, University of Nairobi Faculty of Health Sciences, Nairobi, Nairobi County, 00100, Kenya</aff>
                <aff id="a2">
                    <label>2</label>Department of Psychiatry, University of Nairobi Faculty of Health Sciences, Nairobi, Nairobi County, 00100, Kenya</aff>
                <aff id="a3">
                    <label>3</label>Department of Psychiatry, University of Nairobi Faculty of Health Sciences, Nairobi, Nairobi County, 00100, Kenya</aff>
                <aff id="a4">
                    <label>4</label>Institute of Excellence in Global Health Equity, New York University Grossman School of Medicine, New York, New York, 10016, USA</aff>
                <aff id="a5">
                    <label>5</label>Department of Psychiatry, University of Nairobi Faculty of Health Sciences, Nairobi, Nairobi County, 00100, Kenya</aff>
            </contrib-group>
            <author-notes>
                <corresp id="c1">
                    <label>a</label>
                    <email xlink:href="mailto:amwendwa@kmtc.ac.ke">amwendwa@kmtc.ac.ke</email>
                </corresp>
                <fn fn-type="conflict">
                    <p>No competing interests were disclosed.</p>
                </fn>
            </author-notes>
            <pub-date pub-type="epub">
                <day>30</day>
                <month>4</month>
                <year>2026</year>
            </pub-date>
            <pub-date pub-type="collection">
                <year>2026</year>
            </pub-date>
            <volume>15</volume>
            <elocation-id>652</elocation-id>
            <history>
                <date date-type="accepted">
                    <day>14</day>
                    <month>4</month>
                    <year>2026</year>
                </date>
            </history>
            <permissions>
                <copyright-statement>Copyright: &#x00a9; 2026 C Mwendwa A et al.</copyright-statement>
                <copyright-year>2026</copyright-year>
                <license xlink:href="https://creativecommons.org/licenses/by/4.0/">
                    <license-p>This is an open access article distributed under the terms of the Creative Commons Attribution Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.</license-p>
                </license>
            </permissions>
            <self-uri content-type="pdf" xlink:href="https://f1000research.com/articles/15-652/pdf"/>
            <abstract>
                <sec>
                    <title>Background</title>
                    <p>Adverse childhood experiences are major contributors to mental health disorders which mostly set in during middle adolescence. The study investigates the prevalence of adverse childhood experiences among Kenyan high school students, and their associations with posttraumatic stress disorder, depression and generalised anxiety disorder, and implement a rapid intervention adaptable for resource-limited contexts.</p>
                </sec>
                <sec>
                    <title>Methods</title>
                    <p>A quasi-experimental pretest and post-test design will be utilized and 372 students aged 14&#x2013;18&#x00a0;years in the second year of schooling, from two public high schools will be recruited to participate. The treatment group will undergo the social belonging-wise intervention over three sessions, with the control group remaining passive. Primary outcomes of academic performance, well-being, and loneliness will be measured. Effectiveness of the intervention will be determined using Cohen&#x2019;s d with an anticipated effect size of 0.5 and a high R-squared in multiple linear regression models will indicate stronger associations. Paired t-test will compare reduction of symptoms of the three psychopathologies between the treatment and comparison groups as secondary outcomes.</p>
                </sec>
                <sec>
                    <title>Discussion</title>
                    <p>The goal of the intervention is to alleviate the effects of adverse childhood experiences, enhance academic performance and wellbeing, reduce symptoms of the associated psychopathologies and advocate for improved mental health resources and policies for adolescents.</p>
                </sec>
            </abstract>
            <kwd-group kwd-group-type="author">
                <kwd>adverse childhood experiences</kwd>
                <kwd>posttraumatic stress disorder</kwd>
                <kwd>depression</kwd>
                <kwd>generalized anxiety disorder</kwd>
                <kwd>wise intervention</kwd>
            </kwd-group>
            <funding-group>
                <funding-statement>The author(s) declared that no grants were involved in supporting this work.</funding-statement>
            </funding-group>
        </article-meta>
    </front>
    <body>
        <def-list>
            <title>List of abbreviations</title>
            <def-item>
                <term id="G1">ACEs</term>
                <def>
                    <p>Adverse Childhood Experiences</p>
                </def>
            </def-item>
            <def-item>
                <term id="G2">CPSS-SR-5</term>
                <def>
                    <p>Child PTSD Symptom Scale Self-Report for DSM-5</p>
                </def>
            </def-item>
            <def-item>
                <term id="G3">GAD</term>
                <def>
                    <p>Generalized Anxiety Disorder</p>
                </def>
            </def-item>
            <def-item>
                <term id="G4">GAD-7</term>
                <def>
                    <p>Generalized Anxiety Disorder screener-7</p>
                </def>
            </def-item>
            <def-item>
                <term id="G5">IBM SPSS</term>
                <def>
                    <p>IBM Statistical Packages for Social Sciences</p>
                </def>
            </def-item>
            <def-item>
                <term id="G6">KNH-UoN ERC</term>
                <def>
                    <p>Kenyatta National Hospital-University of Nairobi Ethics Review Committee</p>
                </def>
            </def-item>
            <def-item>
                <term id="G7">LHWs</term>
                <def>
                    <p>Lay Health Workers</p>
                </def>
            </def-item>
            <def-item>
                <term id="G8">LMICs</term>
                <def>
                    <p>Low- and Middle-Income Countries</p>
                </def>
            </def-item>
            <def-item>
                <term id="G9">MHDs</term>
                <def>
                    <p>Mental Health Disorders</p>
                </def>
            </def-item>
            <def-item>
                <term id="G10">NACOSTI</term>
                <def>
                    <p>National Commission for Science, Technology, and Innovation</p>
                </def>
            </def-item>
            <def-item>
                <term id="G11">OSSS-3</term>
                <def>
                    <p>Oslo&#x2019;s Social Support System-3</p>
                </def>
            </def-item>
            <def-item>
                <term id="G12">PHQ-A
</term>
                <def>
                    <p>Patient Health Questionnaire modified for Adolescents</p>
                </def>
            </def-item>
            <def-item>
                <term id="G13">PTSD</term>
                <def>
                    <p>Posttraumatic Stress Disorder</p>
                </def>
            </def-item>
            <def-item>
                <term id="G14">ULS-8</term>
                <def>
                    <p>University of California, Los Angeles (eight-item) Scale</p>
                </def>
            </def-item>
            <def-item>
                <term id="G15">WHO ACE-IQ
</term>
                <def>
                    <p>World Health Organization Adverse Childhood Experiences International Questionnaire</p>
                </def>
            </def-item>
            <def-item>
                <term id="G16">WHO-5</term>
                <def>
                    <p>World Health Organization wellbeing index-5</p>
                </def>
            </def-item>
            <def-item>
                <term id="G17">WIs</term>
                <def>
                    <p>Wise Interventions</p>
                </def>
            </def-item>
        </def-list>
        <sec id="sec4" sec-type="intro">
            <title>Introduction</title>
            <sec id="sec5">
                <title>Mental health disorders and adverse childhood experiences in adolescents</title>
                <p>Mental health disorders (MDHs) affect approximately 14% of adolescents globally and have their onset mostly in middle adolescent years. This contributes to 15% of the global burden of disease, with most of those affected residing in low-and middle-income countries (LMICs).
                    <sup>
                        <xref ref-type="bibr" rid="ref1">1</xref>&#x2013;
                        <xref ref-type="bibr" rid="ref2">2</xref>
                    </sup> Adolescents are at high risk for adverse childhood experiences (ACEs),
                    <sup>
                        <xref ref-type="bibr" rid="ref3">3</xref>
                    </sup> with prevalence rates among high-risk groups of Kenyan adolescents ranging from 60&#x2013;94.8%.
                    <sup>
                        <xref ref-type="bibr" rid="ref4">4</xref>&#x2013;
                        <xref ref-type="bibr" rid="ref5">5</xref>
                    </sup> The impact of ACEs on mental health is influenced by the type, number, and timing of ACEs,
                    <sup>
                        <xref ref-type="bibr" rid="ref6">6</xref>&#x2013;
                        <xref ref-type="bibr" rid="ref11">11</xref>
                    </sup> and is linked to a range of physical, social, emotional, and behavioural issues,
                    <sup>
                        <xref ref-type="bibr" rid="ref12">12</xref>&#x2013;
                        <xref ref-type="bibr" rid="ref14">14</xref>
                    </sup> with significant associations found with posttraumatic stress disorder (PTSD), depression, and anxiety.
                    <sup>
                        <xref ref-type="bibr" rid="ref6">6</xref>,
                        <xref ref-type="bibr" rid="ref14">14</xref>,
                        <xref ref-type="bibr" rid="ref15">15</xref>
                    </sup>
                </p>
                <p>Sociodemographic factors influence exposure to ACEs which often cluster within families, thus increasing the likelihood of multiple experiences
                    <sup>
                        <xref ref-type="bibr" rid="ref16">16</xref>
                    </sup> with adverse experiences in adulthood having effects on children through psychosocial, environmental, and biological pathways, particularly when parents suffer from MHDs.
                    <sup>
                        <xref ref-type="bibr" rid="ref17">17</xref>,
                        <xref ref-type="bibr" rid="ref18">18</xref>
                    </sup> Risk factors for ACEs among Kenyan adolescents include orphanhood, the female gender, low parental education, and living in communities prone to violence.
                    <sup>
                        <xref ref-type="bibr" rid="ref4">4</xref>,
                        <xref ref-type="bibr" rid="ref5">5</xref>,
                        <xref ref-type="bibr" rid="ref19">19</xref>
                    </sup> Despite prevalence rates of PTSD, depression, and anxiety being high among Kenyan adolescents,
                    <sup>
                        <xref ref-type="bibr" rid="ref5">5</xref>,
                        <xref ref-type="bibr" rid="ref19">19</xref>&#x2013;
                        <xref ref-type="bibr" rid="ref21">21</xref>
                    </sup> data on the burden of ACEs in association with these psychopathologies in this population is limited, with many especially those in high school settings remaining unrecognised due to various barriers in mental health care.
                    <sup>
                        <xref ref-type="bibr" rid="ref5">5</xref>,
                        <xref ref-type="bibr" rid="ref22">22</xref>,
                        <xref ref-type="bibr" rid="ref23">23</xref>
                    </sup> Treatment gaps for mental health disorders in LMICs reach up to 90% for children and adolescents, these being significantly higher than rates in high-income countries.
                    <sup>
                        <xref ref-type="bibr" rid="ref24">24</xref>&#x2013;
                        <xref ref-type="bibr" rid="ref26">26</xref>
                    </sup>
                </p>
            </sec>
            <sec id="sec6">
                <title>Interventions for mental health disorders for adolescents</title>
                <p>
Research highlights the adolescent years as critical for school-based mental health interventions,
                    <sup>
                        <xref ref-type="bibr" rid="ref27">27</xref>
                    </sup> with early intervention emphasized as being more effective before academic issues arise, which often signal mental health disorders.
                    <sup>
                        <xref ref-type="bibr" rid="ref28">28</xref>
                    </sup> Social belonging is crucial for self-identity and well-being during adolescence since it significantly influences social development and identity formation.
                    <sup>
                        <xref ref-type="bibr" rid="ref29">29</xref>,
                        <xref ref-type="bibr" rid="ref30">30</xref>
                    </sup> Social support and connectedness help mitigate effects of ACEs,
                    <sup>
                        <xref ref-type="bibr" rid="ref31">31</xref>&#x2013;
                        <xref ref-type="bibr" rid="ref33">33</xref>
                    </sup> and protective factors against loneliness include friendships, school hobbies, societal belonging, various therapies and skill trainings.
                    <sup>
                        <xref ref-type="bibr" rid="ref34">34</xref>&#x2013;
                        <xref ref-type="bibr" rid="ref36">36</xref>
                    </sup> The study advocates for the cost-effective social belonging wise intervention to complement traditional treatments
                    <sup>
                        <xref ref-type="bibr" rid="ref37">37</xref>,
                        <xref ref-type="bibr" rid="ref38">38</xref>
                    </sup> since social support is crucial for enhancing self-esteem
                    <sup>
                        <xref ref-type="bibr" rid="ref32">32</xref>,
                        <xref ref-type="bibr" rid="ref33">33</xref>
                    </sup> which in turn protects against the psychopathologies under this study.
                    <sup>
                        <xref ref-type="bibr" rid="ref39">39</xref>&#x2013;
                        <xref ref-type="bibr" rid="ref47">47</xref>
                    </sup>
                </p>
                <p>The wise interventions (WIs) approach will be utilized since they enhance developmental, academic, and health outcomes for adolescents with MHDs, and due to their brief nature, they are effective in resource-limited settings and complement traditional therapies while reducing stigma through non-explicit labelling of the psychopathologies.
                    <sup>
                        <xref ref-type="bibr" rid="ref22">22</xref>,
                        <xref ref-type="bibr" rid="ref37">37</xref>,
                        <xref ref-type="bibr" rid="ref38">38</xref>
                    </sup> WIs align with the Kenya&#x2019;s Mental Health Policy 2015&#x2013;2030 and the Mental Health Action Plan 2021&#x2013;2025, which focus on support for vulnerable youth and on integrating mental health into school programs and policies.
                    <sup>
                        <xref ref-type="bibr" rid="ref48">48</xref>,
                        <xref ref-type="bibr" rid="ref49">49</xref>
                    </sup>
                </p>
            </sec>
            <sec id="sec7">
                <title>Rationale of the study</title>
                <p>The study investigates the relationship between ACEs, academic performance, well-being, loneliness and three common MHDs namely PTSD, depression, and generalised anxiety disorder (GAD), among Kenyan high school students. It highlights the limited comprehensive local research noting that previous studies often focused on high-risk populations of Kenyan adolescents.
                    <sup>
                        <xref ref-type="bibr" rid="ref4">4</xref>,
                        <xref ref-type="bibr" rid="ref5">5</xref>
                    </sup> The findings aim to affirm that ACEs negatively affect adolescents, supporting evidence from other contexts,
                    <sup>
                        <xref ref-type="bibr" rid="ref50">50</xref>&#x2013;
                        <xref ref-type="bibr" rid="ref58">58</xref>
                    </sup> and emphasizes the importance of understanding the impacts of ACEs on Kenyan adolescents. It explores social belonging as a school-based psychosocial intervention, advocating for a comprehensive preventive approach at the levels of policy, family, community, and schools.
                    <sup>
                        <xref ref-type="bibr" rid="ref59">59</xref>,
                        <xref ref-type="bibr" rid="ref60">60</xref>
                    </sup>
                </p>
            </sec>
        </sec>
        <sec id="sec8">
            <title>Protocol</title>
            <sec id="sec9" sec-type="methods">
                <title>Methods</title>
                <p>

                    <bold>Study design and setting</bold>
                </p>
                <p>A quasi-experimental pretest and post-test design will be utilized in the study. Participants exposed to ACEs from two public boarding high schools in Kenya will be recruited and assigned into treatment and comparison groups by non-random allocation. The comparison group will be chosen from the same school setting using a non-equivalent group design approach to ensure similarity to the treatment group. The treatment group will undergo the intervention while the comparison group will remain passive.</p>
            </sec>
            <sec id="sec10">
                <title>Sample size and sampling procedure</title>
                <p>Purposeful sampling will be used to select the two public boarding high schools and students in the second year of schooling will be recruited from the boys&#x2019; school which has an approximate population of 1280 students, and from the girls&#x2019; school with an approximate population of 1400 students. A systematic sampling approach using class registers will be used for selection of eligible participants. An estimated sample size of 338 students based on the modified Cochran&#x2019;s formula,
                    <sup>
                        <xref ref-type="bibr" rid="ref61">61</xref>
                    </sup> adjusted to 372 students to account for a 10% dropout rate will be used, with 178 drawn from the boys&#x2019; school, and 194 drawn from the girls&#x2019; school. The justification is that for a finite population of the two schools, with a 95% confidence interval (Z&#x00a0;=&#x00a0;1.96), a 5% margin of error, and 50% proportion (p&#x00a0;=&#x00a0;0.5), the sample of 338 is adequate, then adjusted by 10%. Secondly, for an independent samples t-test to detect a medium effect size of 0.5, a power of 0.95, and an alpha of 0.05, a minimum sample of 210 participants will be sufficient, with 105 per group.</p>
                <p>

                    <bold>Eligibility criteria for study participants</bold>
                </p>
                <p>

                    <bold>

                        <italic toggle="yes">Inclusion Criteria</italic>
</bold>

                    <list list-type="order">
                        <list-item>
                            <label>1.</label>
                            <p>Students will be aged between 14 and 18&#x00a0;years, and whose parents will give consent.</p>
                        </list-item>
                        <list-item>
                            <label>2.</label>
                            <p>Students above 18&#x00a0;years who will give consent, and those under 18&#x00a0;years who will give assent.</p>
                        </list-item>
                        <list-item>
                            <label>3.</label>
                            <p>Students who will have ACEs scores of 1&#x2013;13.</p>
                        </list-item>
                    </list>
                </p>
                <p>

                    <bold>

                        <italic toggle="yes">Exclusion Criteria</italic>
</bold>
                </p>
                <p>Students who will have severe depression (these be referred to the school counsellors for further support).</p>
                <p>

                    <bold>Consenting process</bold>
                </p>
                <p>The researcher will meet with school principals to explain the purpose of the study and facilitate obtaining of parental consent after assurance of voluntary participation and confidentiality. After this, consent forms will be distributed to students in sealed envelopes at the end of the school term for parental consenting and returned to the principal&#x2019;s office at the start of the next school term. The researcher and data assistants will then visit the school and distribute consent forms to eligible students if over 18 and assent forms if under 18, explain the content of the forms, and give them time to read through and sign. These will be collected after the exercise. The researcher will request school principals to facilitate selection of six senior students (3 from each school) drawn from the final year of study, who will fill out consent/assent forms and respond to pre-prepared questions. These will be compiled into prewritten accounts that will form part of the intervention.</p>
                <p>

                    <bold>Recruitment and participant enrolment</bold>
                </p>
                <p>The researcher and data assistants will recruit those who meet the eligibility criteria until the desired sample is obtained. To ensure adequate participant enrolment, the researcher will explain the purpose and the benefits of the study to the eligible participants and seek voluntary participation. They will be assured of confidentiality and an assurance that the intervention will be offered to those who fall into the comparison group before study closure. Also, the pre-written accounts obtained from senior students from the respective schools, and the choice of lay health workers (LHWs) who are close in age to the study participants to offer the intervention, and the use of videos with relatable adolescent strategies of belonging as part of the intervention will encourage them to look forward to the sessions. Based on their class streams, participants will be assigned to the treatment, for example streams &#x2018;G&#x2019; and &#x2018;T&#x2019; and comparison groups streams &#x2018;R&#x2019; and &#x2018;S&#x2019;.</p>
                <p>

                    <bold>Confidentiality</bold>
                </p>
                <p>Confidentiality will be maintained using codes for the psychopathologies which will not be revealed to other participants and no personal identifying information will be obtained throughout the study.</p>
                <p>

                    <bold>Study outcomes</bold>
                </p>
                <p>The primary outcomes comparing the treatment and comparison groups pre and post intervention will be:
                    <list list-type="order">
                        <list-item>
                            <label>1.</label>
                            <p>Improved academic performance as depicted by improved mean scores for subjects</p>
                        </list-item>
                        <list-item>
                            <label>2.</label>
                            <p>Improved general well-being scores</p>
                        </list-item>
                        <list-item>
                            <label>3.</label>
                            <p>Reduced loneliness scores</p>
                        </list-item>
                    </list>
                </p>
                <p>The secondary outcomes comparing the treatment and comparison groups pre and post intervention will be the reduction of symptom scores for PTSD, depression, and GAD.</p>
                <p>

                    <bold>Data collection instruments and measures</bold>
                </p>
                <p>A researcher-developed questionnaire will be used to gather 
                    <italic toggle="yes">sociodemographic characteristics</italic> of study participants, which will include age, gender, year of study, religion, family setup, parents&#x2019; highest education level, parents&#x2019; employment status, type of housing, and location of residence if urban or rural. 
                    <italic toggle="yes">ACE types and scores</italic>, will be assessed using the WHO ACEs International Questionnaire (WHO ACE IQ)
                    <sup>
                        <xref ref-type="bibr" rid="ref62">62</xref>
                    </sup> with binary scores ranging from 0 to 13 of which four or more indicate a high ACE burden. It demonstrated good psychometric properties in Malawian adolescents
                    <sup>
                        <xref ref-type="bibr" rid="ref63">63</xref>
                    </sup> and positively correlated with the original 10-item ACEs questionnaire used in Kenyan adults.
                    <sup>
                        <xref ref-type="bibr" rid="ref64">64</xref>
                    </sup> 
                    <italic toggle="yes">Well-being
</italic> will be assessed using the WHO-5 wellbeing index (WHO-5), a 6-point Likert scale (0 to 5), with raw scores from 0 to 25 and converted to a percentage with higher scores indicating better well-being.
                    <sup>
                        <xref ref-type="bibr" rid="ref65">65</xref>
                    </sup> The scale showed adequate reliability of Cronbach&#x2019;s alpha 0.86 and 0.75 among Ghanaian adolescents and Kenyan adolescents
                    <sup>
                        <xref ref-type="bibr" rid="ref66">66</xref>,
                        <xref ref-type="bibr" rid="ref67">67</xref>
                    </sup> respectively. 
                    <italic toggle="yes">Feelings of loneliness</italic> will be assessed using the UCLA Loneliness scale-8 (ULS-8) with scores ranging from 8 to 32, higher scores indicating greater loneliness.
                    <sup>
                        <xref ref-type="bibr" rid="ref68">68</xref>
                    </sup> It showed good convergent validity with other loneliness measures and construct validity, among Kenyan adolescents who had depression and anxiety (Cronbach &#x03b1;&#x00a0;=&#x00a0;.74).
                    <sup>
                        <xref ref-type="bibr" rid="ref69">69</xref>
                    </sup> 
                    <italic toggle="yes">PTSD</italic> will be evaluated using the Child PTSD Symptom Scale Self-Report (CPSS-5 SR), with severity scores ranging from minimal (0&#x2013;10) to very severe (61&#x2013;80).
                    <sup>
                        <xref ref-type="bibr" rid="ref70">70</xref>
                    </sup> It demonstrated excellent psychometric properties of moderate test-retest reliability (.62&#x2013;.68) in Zambian adolescents,
                    <sup>
                        <xref ref-type="bibr" rid="ref71">71</xref>
                    </sup> correlating.80 with the Child&#x2019;s PTSD checklist used in Kenya high school students.
                    <sup>
                        <xref ref-type="bibr" rid="ref72">72</xref>
                    </sup>
                </p>
                <p>

                    <italic toggle="yes">Depression</italic> will be assessed using the Patient Health Questionnaire 9 modified for Adolescents (PHQ-A), with severity cut-offs of 5&#x2013;9 for mild, 10&#x2013;14 for moderate, 15&#x2013;19 for moderately severe, and 20&#x2013;27 for severe depression.
                    <sup>
                        <xref ref-type="bibr" rid="ref73">73</xref>
                    </sup> Psychometric properties of the English and Kiswahili versions are comparable, with internal consistency values of 0.862 and 0.834, respectively among Kenyan adolescents.
                    <sup>
                        <xref ref-type="bibr" rid="ref74">74</xref>
                    </sup> The Generalized Anxiety Disorder screener (GAD-7), will be used to assess for 
                    <italic toggle="yes">anxiety</italic> levels, categorized as mild (5&#x2013;9), moderate (10&#x2013;14), or severe (15&#x2013;21).
                    <sup>
                        <xref ref-type="bibr" rid="ref75">75</xref>
                    </sup> It showed good internal consistency and effectively identified suicide risk and depression in Ghanaian adolescents and Kenyan adolescents (Cronbach&#x2019;s alpha&#x00a0;=&#x00a0;0.69 and 0.70) respectively.
                    <sup>
                        <xref ref-type="bibr" rid="ref22">22</xref>,
                        <xref ref-type="bibr" rid="ref76">76</xref>
                    </sup> 
                    <italic toggle="yes">Social support</italic> in schools will be measured as a mediating variable using the Oslo&#x2019;s Social Support Scale (OSSS-3) categorizing scores as 3&#x2013;8 for poor support, 9&#x2013;11 for moderate support, and 12&#x2013;14 for strong support.
                    <sup>
                        <xref ref-type="bibr" rid="ref77">77</xref>
                    </sup> It demonstrated convergent and discriminant validity for family support and discriminant validity for support by friends for mental health disorders among Ghanaian adolescents,
                    <sup>
                        <xref ref-type="bibr" rid="ref44">44</xref>
                    </sup> had good psychometric properties in linking social support to depression in Ethiopian adolescents
                    <sup>
                        <xref ref-type="bibr" rid="ref78">78</xref>
                    </sup> and demonstrated a strong correlation with the 12-item Multidimensional Scale of Perceived Social Support, validated in Kenyan adolescents (Cronbach&#x2019;s &#x03b1; 0.86).
                    <sup>
                        <xref ref-type="bibr" rid="ref22">22</xref>
                    </sup> 
                    <italic toggle="yes">Academic performance</italic> will be evaluated through comparison of mean scores of term marks across subjects from three periods: term three (September to October 2025, pre-intervention), term one (January to March 2026, during intervention), and term two (May to July 2025, post-intervention) between the treatment and comparison groups.</p>
                <p>

                    <bold>Data collection procedures</bold>
                </p>
                <p>

                    <bold>

                        <italic toggle="yes">Pretesting</italic>
</bold>
                </p>
                <p>Pretesting of the data collection instruments will be conducted at a different public high school involving twenty students in the second year of study.</p>
                <p>

                    <bold>Preintervention stage and measures</bold>
                </p>
                <p>

                    <bold>

                        <italic toggle="yes">Recruiting and training of lay health workers and data assistants</italic>
</bold>
                </p>
                <p>The researcher will develop a training manual for the LHWs, recruit them from recent college/university graduates, and train them on delivery of the intervention.</p>
                <p>Eligibility criteria for LHWs will be:
                    <list list-type="order">
                        <list-item>
                            <label>1.</label>
                            <p>Aged between 23 and 25&#x00a0;years</p>
                        </list-item>
                        <list-item>
                            <label>2.</label>
                            <p>Having attended a Kenyan high school</p>
                        </list-item>
                        <list-item>
                            <label>3.</label>
                            <p>Fluent in English and Kiswahili</p>
                        </list-item>
                        <list-item>
                            <label>4.</label>
                            <p>Be a recent graduate of either a university/college</p>
                        </list-item>
                        <list-item>
                            <label>5.</label>
                            <p>Have good interpersonal skills</p>
                        </list-item>
                        <list-item>
                            <label>6.</label>
                            <p>Have an interest in working with high school students</p>
                        </list-item>
                    </list>
                </p>
                <p>Similarly, data assistants will be recruited from recent college/university graduates, undergo an online research ethics course for certification before being trained on recruitment, informed consent, group allocation, administration of the assessment scales, and data management. A follow-up on their skills will be done two weeks post-training.</p>
                <p>

                    <bold>

                        <italic toggle="yes">Quality assurance procedures</italic>
</bold>
                </p>
                <p>Quality assurance for the LHWs will include follow-up sessions with a psychologist and the researcher, held two weeks after training. These sessions will aim to reinforce and monitor skills acquired on delivery of the intervention. Quality assurance by the researcher for the data assistants will involve follow-up sessions focusing on recruitment, informed consent role plays, and data management procedures. Regular checks will ensure data accuracy, with missing data issues will be communicated to the data assistants to reduce their occurrence.</p>
                <p>

                    <bold>

                        <italic toggle="yes">Intervention fidelity</italic>
</bold>
                </p>
                <p>Independent assessors will receive training from the researcher regarding the intervention and assessment rubric for the LHWs. The assessment will focus on two aspects: adherence to the intervention protocol, graded as &#x201c;N&#x201d; for no or &#x201c;Y&#x201d; for yes, and competence in delivering the intervention, evaluated on a scale of 1 to 3. The assessors will give feedback to LHWs following each session.</p>
                <p>

                    <bold>

                        <italic toggle="yes">Preintervention measures</italic>
</bold>
                </p>
                <p>Pretests will include the sociodemographic questionnaire, the WHO ACE IQ questionnaire, the CPPS-5 SR, the PHQ-A, the GAD-7, the WHO-5 wellbeing, the ULS -8 loneliness and the OSSS-3 scales; and preintervention academic reports.</p>
                <p>

                    <bold>Harms definition and assessment</bold>
                </p>
                <p>Harms will be signified by psychological distress such as looking anxious, looking disinterested or crying because some of the questions in the WHO ACE IQ questionnaire and the CPPS-5 SR scale may be intrusive. These signs will be assessed for by the LHWs and those affected will be referred to the psychologist on the team and the school counsellor for further support. This will be done through the different stages of engagement with the participants.</p>
                <p>

                    <bold>The intervention stage and intervention components</bold>
                </p>
                <p>The researcher will divide the participants into groups of 15 prior to the sessions, and the LHWs will deliver the interventions to their allocated group in three sessions at one monthly intervals with each intervention lasting one hour. The three sessions of the interventions are designed to help participants develop a sense of social belonging and will be as follows: the 
                    <italic toggle="yes">first session</italic> will include establishing rapport, presentation of the goals and benefits of the intervention, video presentations on what belonging is, reading of a senior student&#x2019;s pre-written account on belonging, and a discussion on practical ways to belong in school. The 
                    <italic toggle="yes">second session</italic> will aim to foster comprehension of useful strategies for school belonging and will entail a review of the goals and benefits of the intervention, videos on struggles faced by students in belonging and the connection between belonging, effort, and time. This will be followed by reading of a senior student&#x2019;s pre-written account that promotes belonging and a discussion to reinforce the participants&#x2019; understanding of the content of the session. The 
                    <italic toggle="yes">third session</italic> will emphasize the importance of belonging to the school community through a video, a senior student&#x2019;s account and a first-hand story from the LHW of their experience of belonging to their school community. The session will be concluded with a discussion focusing on the participants view of the benefits of belonging to community.</p>
                <p>

                    <bold>Participants&#x2019; adherence and retention</bold>
                </p>
                <p>To promote participant adherence to the intervention, retention and complete follow-up, the researcher will send reminders of the scheduled sessions through the school counsellor and class teacher one week prior to the intervention and hold the sessions during the free times for the participants. If any participants deviate or drop out, the reason will be sought through the school counsellors and addressed.</p>
                <p>

                    <bold>Post intervention stage and measures</bold>
                </p>
                <p>Post-tests will include the CPSS-5 SR, the PHQ-A, the GAD-7, the WHO-5 Well-being, the ULS-8 loneliness, and the OSSS-3 scales; and academic reports for both groups obtained one month post intervention. The intervention will be administered to the comparison group before closure of the study so the group benefits. 
                    <xref ref-type="table" rid="T1">
Table 1</xref> shows the schedule of study visits and activities and 
                    <xref ref-type="fig" rid="f1">
Figure 1</xref> shows the flowchart for the recruitment and follow-up processes.</p>
                <table-wrap id="T1" orientation="portrait" position="float">
                    <label>
Table 1. </label>
                    <caption>
                        <title>Schedule for the study visits and activities.</title>
                    </caption>
                    <table content-type="article-table" frame="hsides">
                        <thead>
                            <tr>
                                <th align="left" colspan="1" rowspan="1" valign="top">Activity</th>
                                <th align="left" colspan="1" rowspan="1" valign="top">V1</th>
                                <th align="left" colspan="1" rowspan="1" valign="top">V2</th>
                                <th align="left" colspan="1" rowspan="1" valign="top">V3</th>
                                <th align="left" colspan="1" rowspan="1" valign="top">V4</th>
                                <th align="left" colspan="1" rowspan="1" valign="top">V5</th>
                                <th align="left" colspan="1" rowspan="1" valign="top">V6</th>
                                <th align="left" colspan="1" rowspan="1" valign="top">V7</th>
                                <th align="left" colspan="1" rowspan="1" valign="top">V8</th>
                            </tr>
                        </thead>
                        <tbody>
                            <tr>
                                <td align="left" colspan="1" rowspan="1" valign="top">Informed consent/assent</td>
                                <td align="left" colspan="1" rowspan="1" valign="top">&#x221a;</td>
                                <td colspan="1" rowspan="1"/>
                                <td colspan="1" rowspan="1"/>
                                <td colspan="1" rowspan="1"/>
                                <td colspan="1" rowspan="1"/>
                                <td colspan="1" rowspan="1"/>
                                <td colspan="1" rowspan="1"/>
                                <td colspan="1" rowspan="1"/>
                            </tr>
                            <tr>
                                <td align="left" colspan="1" rowspan="1" valign="top">ACE, PTSD, depression &amp; anxiety screening &amp; sociodemographic questionnaire</td>
                                <td colspan="1" rowspan="1"/>
                                <td align="left" colspan="1" rowspan="1" valign="top">&#x221a;</td>
                                <td colspan="1" rowspan="1"/>
                                <td colspan="1" rowspan="1"/>
                                <td colspan="1" rowspan="1"/>
                                <td colspan="1" rowspan="1"/>
                                <td colspan="1" rowspan="1"/>
                                <td colspan="1" rowspan="1"/>
                            </tr>
                            <tr>
                                <td align="left" colspan="1" rowspan="1" valign="top">Inclusion/exclusion criteria</td>
                                <td colspan="1" rowspan="1"/>
                                <td align="left" colspan="1" rowspan="1" valign="top">&#x221a;</td>
                                <td colspan="1" rowspan="1"/>
                                <td colspan="1" rowspan="1"/>
                                <td colspan="1" rowspan="1"/>
                                <td colspan="1" rowspan="1"/>
                                <td colspan="1" rowspan="1"/>
                                <td colspan="1" rowspan="1"/>
                            </tr>
                            <tr>
                                <td align="left" colspan="1" rowspan="1" valign="top">Group allocation</td>
                                <td colspan="1" rowspan="1"/>
                                <td align="left" colspan="1" rowspan="1" valign="top">&#x221a;</td>
                                <td colspan="1" rowspan="1"/>
                                <td colspan="1" rowspan="1"/>
                                <td colspan="1" rowspan="1"/>
                                <td colspan="1" rowspan="1"/>
                                <td colspan="1" rowspan="1"/>
                                <td colspan="1" rowspan="1"/>
                            </tr>
                            <tr>
                                <td align="left" colspan="1" rowspan="1" valign="top">Oslo&#x2019;s social support system screening</td>
                                <td colspan="1" rowspan="1"/>
                                <td colspan="1" rowspan="1"/>
                                <td align="left" colspan="1" rowspan="1" valign="top">&#x221a;</td>
                                <td colspan="1" rowspan="1"/>
                                <td colspan="1" rowspan="1"/>
                                <td colspan="1" rowspan="1"/>
                                <td align="left" colspan="1" rowspan="1" valign="top">&#x221a;</td>
                                <td colspan="1" rowspan="1"/>
                            </tr>
                            <tr>
                                <td align="left" colspan="1" rowspan="1" valign="top">WHO well-being screen</td>
                                <td colspan="1" rowspan="1"/>
                                <td colspan="1" rowspan="1"/>
                                <td align="left" colspan="1" rowspan="1" valign="top">&#x221a;</td>
                                <td colspan="1" rowspan="1"/>
                                <td colspan="1" rowspan="1"/>
                                <td colspan="1" rowspan="1"/>
                                <td align="left" colspan="1" rowspan="1" valign="top">&#x221a;</td>
                                <td colspan="1" rowspan="1"/>
                            </tr>
                            <tr>
                                <td align="left" colspan="1" rowspan="1" valign="top">ULS &#x2013; 8 loneliness scale</td>
                                <td colspan="1" rowspan="1"/>
                                <td colspan="1" rowspan="1"/>
                                <td align="left" colspan="1" rowspan="1" valign="top">&#x221a;</td>
                                <td colspan="1" rowspan="1"/>
                                <td colspan="1" rowspan="1"/>
                                <td colspan="1" rowspan="1"/>
                                <td align="left" colspan="1" rowspan="1" valign="top">&#x221a;</td>
                                <td colspan="1" rowspan="1"/>
                            </tr>
                            <tr>
                                <td align="left" colspan="1" rowspan="1" valign="top">Academic performance (end of Term Results for 3 consecutive Terms)</td>
                                <td colspan="1" rowspan="1"/>
                                <td colspan="1" rowspan="1"/>
                                <td align="left" colspan="1" rowspan="1" valign="top">&#x221a;</td>
                                <td colspan="1" rowspan="1"/>
                                <td colspan="1" rowspan="1"/>
                                <td align="left" colspan="1" rowspan="1" valign="top">&#x221a;</td>
                                <td align="left" colspan="1" rowspan="1" valign="top">&#x221a;</td>
                                <td colspan="1" rowspan="1"/>
                            </tr>
                            <tr>
                                <td align="left" colspan="1" rowspan="1" valign="top">PTSD, depression &amp; anxiety screening</td>
                                <td colspan="1" rowspan="1"/>
                                <td colspan="1" rowspan="1"/>
                                <td colspan="1" rowspan="1"/>
                                <td colspan="1" rowspan="1"/>
                                <td colspan="1" rowspan="1"/>
                                <td colspan="1" rowspan="1"/>
                                <td align="left" colspan="1" rowspan="1" valign="top">&#x221a;</td>
                                <td colspan="1" rowspan="1"/>
                            </tr>
                            <tr>
                                <td align="left" colspan="1" rowspan="1" valign="top">Social belonging wise intervention</td>
                                <td colspan="1" rowspan="1"/>
                                <td colspan="1" rowspan="1"/>
                                <td colspan="1" rowspan="1"/>
                                <td align="left" colspan="1" rowspan="1" valign="top">&#x221a; (RX group)</td>
                                <td align="left" colspan="1" rowspan="1" valign="top">&#x221a; (R
                                    <sub>X</sub> group)</td>
                                <td align="left" colspan="1" rowspan="1" valign="top">&#x221a; (R
                                    <sub>X</sub> group)</td>
                                <td colspan="1" rowspan="1"/>
                                <td align="left" colspan="1" rowspan="1" valign="top">&#x221a; (comparison group)</td>
                            </tr>
                        </tbody>
                    </table>
                    <table-wrap-foot>
                        <p>Abbreviations: ACE: adverse childhood experiences, PTSD; posttraumatic stress disorder; WHO; World Health Organization; ULS: ULS-8: University of California, Los Angeles (eight-item) Loneliness scale; R
                            <sub>X:</sub> Treatment group; V; number of visits</p>
                    </table-wrap-foot>
                </table-wrap>
                <fig fig-type="figure" id="f1" orientation="portrait" position="float">
                    <label>
Figure 1. </label>
                    <caption>
                        <title>Consort diagram for the flowchart of the recruitment and follow up processes.</title>
                    </caption>
                    <graphic id="gr1" orientation="portrait" position="float" xlink:href="https://f1000research-files.f1000.com/manuscripts/198261/169497e3-576b-488b-93f0-e32ca582d846_figure1.gif"/>
                </fig>
                <p>

                    <bold>Data storage and security</bold>
                </p>
                <p>Data will be in the form of filled out questionnaires and these will be stored in labelled files for the respective schools under lock and key. Data will be cleaned, coded and entered into EpiData Entry version 3.1, then exported to IBM SPSS Statistics version 29 for analysis. This will be saved in a laptop that will be accessible to the researcher and the statistician once entered, then backed up in iCloud.</p>
                <p>

                    <bold>Data analysis techniques</bold>
                </p>
                <p>All tools except the Oslo Social Support-3 scale have been validated in the Kenyan context and will be tested pre-intervention. Reliability will be assessed using Cronbach&#x2019;s alpha, targeting &#x2265;0.7 internal consistency. Normality testing and skew kurtosis analysis will precede testing for assumptions of multiple linear regression. Missing data will be imputed with the k-Nearest Neighbours algorithm. Descriptive statistics will include frequencies, percentages, and measures of central tendency for sociodemographic characteristics, ACE scores, and Oslo social support. The chi-square test will be utilized to analyze differences in ACE category distributions by gender and PTSD, depression, and GAD scores in the treatment and control groups. Cohen&#x2019;s d will evaluate the effect of the intervention on academic performance, general well-being and loneliness, with an expected effect size of 0.5. Inferential statistics will evaluate the relationships between the intervention, academic performance, general well-being and loneliness using a significance level of p-value 0.05 and confidence interval set at 95%. Multiple linear regression models will assess the strength of these associations while controlling for ACEs and social demographic characteristics as moderating variables, and Oslo&#x2019;s social support as a mediating variable. High R-squared values will indicate stronger associations. Paired t-tests will assess symptom reduction in PTSD, depression, and anxiety between treatment and comparison groups across beginning point and endpoint assessments.</p>
                <p>

                    <bold>Dissemination</bold>
                </p>
                <p>The results of the study will be presented at the University of Nairobi&#x2019;s Department of Psychiatry, and a summary report will be done for the Ministry of Education, the Ministry of Health and the school administrations. The findings will also be presented at scientific conferences and published in peer-reviewed journals and shared with the National Commission for Science, Technology, and Innovation (NACOSTI) and the University of Nairobi library and repository.</p>
                <p>

                    <bold>Study status</bold>
                </p>
                <p>Recruitment of study participants and data collection began on 13
                    <sup>th</sup> October 2025. Data collection is ongoing and is planned to be concluded on 31
                    <sup>st</sup>August 2026, with results anticipated by October 2026.</p>
            </sec>
        </sec>
        <sec id="sec11" sec-type="discussion">
            <title>Discussion</title>
            <sec id="sec12">
                <title>Expected outcomes and benefits of the study</title>
                <p>Adolescents in sub-Saharan Africa experience a significant prevalence of ACEs and mental health disorders which often remaining undiagnosed. This study seeks to enhance care through the social belonging wise intervention that is expected to give better academic outcomes, improve general well-being, reduce loneliness and symptoms of PTSD, depression and GAD. Culturally relevant concepts of belonging will be integrated into school environments and findings will inform policymakers on the prevalence of ACEs among Kenyan high school adolescents. The outcomes will guide the development of ACEs prevention and management policies and improve adolescent mental health care systems. The data gathered will support resource allocation, increase awareness of effects of ACEs on youth mental health disorders, and promote mental health discussions in schools, ultimately supporting counselling programs. The research also will set the groundwork for future interventions aimed at reducing the mental health disorder burden associated with ACEs in Kenyan youth.</p>
                <p>In conclusion, the study is particularly valuable in a LMIC context where adolescents face a significant burden of ACEs and MHDs amidst challenges in accessing mental health care.</p>
            </sec>
            <sec id="sec13">
                <title>Limitations</title>
                <p>The limitations are overestimation of the effect size since there is no randomization and thus there will be need to control for confounding variables to improve internal validity. A second limitation is selection bias which will be reduced by selecting a comparison group that has similar characteristics to the treatment group. Thirdly, since the study will be carried out in two high schools, the results will not be generalizable to all Kenyan high school students.</p>
            </sec>
        </sec>
        <sec id="sec14">
            <title>Ethics considerations</title>
            <p>Ethical approval was obtained from the Kenyatta National Hospital &#x2013; University of Nairobi) Ethics and Research Committee (KNH-UoN ERC P59/02/2024) dated 26.06.2024, and NACOSTI issued a permit. In accordance with the Declaration of Helsinki, only participants who give written consent will be included in the study.</p>
        </sec>
        <sec id="sec15">
            <title>Consent for publication</title>
            <p>Not applicable.</p>
        </sec>
    </body>
    <back>
        <sec id="sec18" sec-type="data-availability">
            <title>Data and software availability</title>
            <p>No data are associated with this article.</p>
            <p>Name of repository: OSF Registries, 
                <ext-link ext-link-type="uri" xlink:href="https://creativecommons.org/publicdomain/zero/1.0/legalcode">License CC0 1.0 Universal</ext-link> 
                <ext-link ext-link-type="uri" xlink:href="https://doi.org/10.17605/OSF.IO/GTES8">https://doi.org/10.17605/OSF.IO/GTES8</ext-link>.
                <sup>
                    <xref ref-type="bibr" rid="ref79">79</xref>
                </sup>
            </p>
            <p>

                <ext-link ext-link-type="uri" xlink:href="https://doi.org/10.17605/OSF.IO/P98TG ">https://doi.org/10.17605/OSF.IO/P98TG </ext-link> (associated project)
                <list list-type="bullet">
                    <list-item>
                        <label>&#x2022;</label>
                        <p>Check list title: SPIRIT checklist</p>
                    </list-item>
                </list>
            </p>
        </sec>
        <ack>
            <title>Acknowledgments</title>
            <p>The authors would like to thank the principals of the two public high schools that will be used as study sites and the high school that will be used for pretesting the data collecting tools for allowing the research to carried out in the schools. Also, the teachers and the counsellors for the logistical and counselling support and all students who will participate in the study.</p>
        </ack>
        <ref-list>
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