<?xml version="1.0" encoding="UTF-8"?><!DOCTYPE article PUBLIC "-//NLM//DTD JATS (Z39.96) Journal Publishing DTD v1.2 20190208//EN" "http://jats.nlm.nih.gov/publishing/1.2/JATS-journalpublishing1.dtd"><article xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" article-type="systematic-review" dtd-version="1.2" xml:lang="en">
    <front>
        <journal-meta>
            <journal-id journal-id-type="pmc">F1000Research</journal-id>
            <journal-title-group>
                <journal-title>F1000Research</journal-title>
            </journal-title-group>
            <issn pub-type="epub">2046-1402</issn>
            <publisher>
                <publisher-name>F1000 Research Limited</publisher-name>
                <publisher-loc>London, UK</publisher-loc>
            </publisher>
        </journal-meta>
        <article-meta>
            <article-id pub-id-type="doi">10.12688/f1000research.180136.1</article-id>
            <article-categories>
                <subj-group subj-group-type="heading">
                    <subject>Systematic Review</subject>
                </subj-group>
                <subj-group>
                    <subject>Articles</subject>
                </subj-group>
            </article-categories>
            <title-group>
                <article-title>Impact of Nutritional and Organoleptic Use of Underutilized Green Leafy Vegetables in Bakery Products: A Systematic Review of Novel Food Applications.</article-title>
                <fn-group content-type="pub-status">
                    <fn>
                        <p>[version 1; peer review: 1 approved, 2 approved with reservations]</p>
                    </fn>
                </fn-group>
            </title-group>
            <contrib-group>
                <contrib contrib-type="author" corresp="no">
                    <name>
                        <surname>Santhanam</surname>
                        <given-names>Madhusudhanan</given-names>
                    </name>
                    <role content-type="http://credit.niso.org/">Investigation</role>
                    <role content-type="http://credit.niso.org/">Methodology</role>
                    <role content-type="http://credit.niso.org/">Writing &#x2013; Original Draft Preparation</role>
                    <uri content-type="orcid">https://orcid.org/0009-0000-2296-3199</uri>
                    <xref ref-type="aff" rid="a1">1</xref>
                </contrib>
                <contrib contrib-type="author" corresp="yes">
                    <name>
                        <surname>Ga</surname>
                        <given-names>Somu</given-names>
                    </name>
                    <role content-type="http://credit.niso.org/">Conceptualization</role>
                    <role content-type="http://credit.niso.org/">Writing &#x2013; Review &amp; Editing</role>
                    <uri content-type="orcid">https://orcid.org/0000-0003-3969-5121</uri>
                    <xref ref-type="corresp" rid="c1">a</xref>
                    <xref ref-type="aff" rid="a2">2</xref>
                </contrib>
                <contrib contrib-type="author" corresp="no">
                    <name>
                        <surname>Piramanayagam</surname>
                        <given-names>Senthil Kumaran</given-names>
                    </name>
                    <role content-type="http://credit.niso.org/">Visualization</role>
                    <uri content-type="orcid">https://orcid.org/0000-0001-6746-0421</uri>
                    <xref ref-type="aff" rid="a3">3</xref>
                </contrib>
                <contrib contrib-type="author" corresp="no">
                    <name>
                        <surname>Sukumar</surname>
                        <given-names>Suresh</given-names>
                    </name>
                    <role content-type="http://credit.niso.org/">Writing &#x2013; Review &amp; Editing</role>
                    <uri content-type="orcid">https://orcid.org/0000-0001-9345-9790</uri>
                    <xref ref-type="aff" rid="a4">4</xref>
                </contrib>
                <contrib contrib-type="author" corresp="no">
                    <name>
                        <surname>Theruveethi</surname>
                        <given-names>Nagarajan</given-names>
                    </name>
                    <role content-type="http://credit.niso.org/">Writing &#x2013; Review &amp; Editing</role>
                    <uri content-type="orcid">https://orcid.org/0000-0003-1028-5807</uri>
                    <xref ref-type="aff" rid="a5">5</xref>
                </contrib>
                <contrib contrib-type="author" corresp="no">
                    <name>
                        <surname>Kumaresan</surname>
                        <given-names>Anusha Priyadarsini</given-names>
                    </name>
                    <role content-type="http://credit.niso.org/">Supervision</role>
                    <uri content-type="orcid">https://orcid.org/0009-0000-2866-5176</uri>
                    <xref ref-type="aff" rid="a6">6</xref>
                </contrib>
                <contrib contrib-type="author" corresp="no">
                    <name>
                        <surname>Ahmed Jalaludeen</surname>
                        <given-names>Nishar</given-names>
                    </name>
                    <role content-type="http://credit.niso.org/">Validation</role>
                    <xref ref-type="aff" rid="a7">7</xref>
                </contrib>
                <contrib contrib-type="author" corresp="no">
                    <name>
                        <surname>Dhandapani</surname>
                        <given-names>Rajmohan</given-names>
                    </name>
                    <role content-type="http://credit.niso.org/">Data Curation</role>
                    <role content-type="http://credit.niso.org/">Investigation</role>
                    <uri content-type="orcid">https://orcid.org/0000-0002-5938-5086</uri>
                    <xref ref-type="aff" rid="a8">8</xref>
                </contrib>
                <contrib contrib-type="author" corresp="no">
                    <name>
                        <surname>Vijayasarathi</surname>
                        <given-names>Guruprasad</given-names>
                    </name>
                    <role content-type="http://credit.niso.org/">Visualization</role>
                    <uri content-type="orcid">https://orcid.org/0000-0002-7144-9652</uri>
                    <xref ref-type="aff" rid="a9">9</xref>
                </contrib>
                <aff id="a1">
                    <label>1</label>Department of Culinary Arts,Welcomgroup Graduate School of Hotel Administration, Manipal Academy of Higher Education, Manipal, Karnataka, 576104, India</aff>
                <aff id="a2">
                    <label>2</label>Department of Hospital Administration, Kasturba Medical College,, Manipal Academy of Higher Education, Manipal, Karnataka, 576104, India</aff>
                <aff id="a3">
                    <label>3</label>Department of Allied Hospitality Studies, Welcomgroup Graduate School of Hotel Administration, Manipal Academy of Higher Education, Manipal, Karnataka, 576104, India</aff>
                <aff id="a4">
                    <label>4</label>Department of Medical Imaging Technology, Manipal College of Health Professions, Manipal Academy of Higher Education, Manipal, Karnataka, 576104, India</aff>
                <aff id="a5">
                    <label>5</label>Department of Optometry. Manipal College of Health Professions, Manipal Academy of Higher Education, Manipal, Karnataka, 576104, India</aff>
                <aff id="a6">
                    <label>6</label>Department of Dietetics and Applied Nutrition, Welcomgroup Graduate School of Hotel Administration, Manipal Academy of Higher Education, Manipal, Karnataka, 576104, India</aff>
                <aff id="a7">
                    <label>7</label>Department of Food and Beverage Service, Welcomgroup Graduate School of Hotel Administration, Manipal Academy of Higher Education, Manipal, Karnataka, 576104, India</aff>
                <aff id="a8">
                    <label>8</label>Department of Culinary Arts, Welcomgroup Graduate School of Hotel Administration, Manipal Academy of Higher Education, Manipal, Karnataka, 576104, India</aff>
                <aff id="a9">
                    <label>9</label>School of Rehabilitation and Medical Sciences, College of Health Sciences, University of Nizwa, Nizwa,Sultanate of, Oman</aff>
            </contrib-group>
            <author-notes>
                <corresp id="c1">
                    <label>a</label>
                    <email xlink:href="mailto:somu.g@manipal.edu">somu.g@manipal.edu</email>
                </corresp>
                <fn fn-type="conflict">
                    <p>No competing interests were disclosed.</p>
                </fn>
            </author-notes>
            <pub-date pub-type="epub">
                <day>14</day>
                <month>5</month>
                <year>2026</year>
            </pub-date>
            <pub-date pub-type="collection">
                <year>2026</year>
            </pub-date>
            <volume>15</volume>
            <elocation-id>732</elocation-id>
            <history>
                <date date-type="accepted">
                    <day>21</day>
                    <month>4</month>
                    <year>2026</year>
                </date>
            </history>
            <permissions>
                <copyright-statement>Copyright: &#x00a9; 2026 Santhanam M et al.</copyright-statement>
                <copyright-year>2026</copyright-year>
                <license xlink:href="https://creativecommons.org/licenses/by/4.0/">
                    <license-p>This is an open access article distributed under the terms of the Creative Commons Attribution Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.</license-p>
                </license>
            </permissions>
            <self-uri content-type="pdf" xlink:href="https://f1000research.com/articles/15-732/pdf"/>
            <abstract>
                <sec>
                    <title>Background</title>
                    <p>The low utilization of green leafy vegetables (GLVs) makes them a nutrient-dense food that is frequently overlooked in the food system. Their integration into baked products may confer nutritional and organizational benefits. However, few studies have addressed their impact on bakery formulations.</p>
                </sec>
                <sec>
                    <title>Objectives</title>
                    <p>This systematic review aimed to jointly appraise the nutritional enrichment and sensory properties of bakery products enhanced with the incorporation of underutilized GLVs, and to highlight their potential new food and industrial applications.</p>
                </sec>
                <sec>
                    <title>Methods</title>
                    <p>Systematic searches were conducted in scientific databases, PubMed, Scopus, Google Scholar, and Web of Science, according to the PRISMA guidelines. Only primary peer-reviewed journal articles that focused on assessing the nutritional profile, sensory attributes, and technological feasibility of incorporating GLVs into bakery products were selected for this review. We documented the micronutrient content, bioactive components, consumer acceptability, and technological challenges.</p>
                </sec>
                <sec>
                    <title>Results</title>
                    <p>One significant difference found in food products was that GLV incorporation increased essential nutrients. Bioactive compounds from GLVs offer health benefits including improved glycemic control and reduced oxidative stress. However, sensory acceptability remains an issue, with bitterness and texture as main obstacles. Consumer studies show acceptable GLV levels depend on species and processing methods. Techniques such as dehydration and fermentation mitigate unwanted sensory effects.</p>
                </sec>
                <sec>
                    <title>Conclusions</title>
                    <p>As we incorporate more underutilised green leafy vegetables in bakery products, it becomes increasingly evident that they have substantial potential to provide significant nutritional and functional benefits to humans. Future research should address the fine-tuning of formulations to maximize benefits while meeting consumer preferences and ensuring commercial feasibility on a large scale in the food industry.</p>
                </sec>
                <sec>
                    <title>Registration</title>
                    <p>This systematic review was registered with the Open Science Framework (OSF). DOI: 
                        <uri xlink:href="http://dx.doi.org/10.17605/OSF.IO/7YHDZ">https://doi.org/10.17605/OSF.IO/7YHDZ</uri>.</p>
                </sec>
            </abstract>
            <kwd-group kwd-group-type="author">
                <kwd>Underutilized Green Leafy Vegetables (GLVs)</kwd>
                <kwd>Bakery Product Fortification</kwd>
                <kwd>Nutritional Enhancement</kwd>
                <kwd>Sensory Acceptability</kwd>
                <kwd>Functional Food Applications.</kwd>
            </kwd-group>
            <funding-group>
                <funding-statement>The author(s) declared that no grants were involved in supporting this work.</funding-statement>
            </funding-group>
        </article-meta>
    </front>
    <body>
        <sec id="sec7" sec-type="intro">
            <title>Introduction</title>
            <sec id="sec8">
                <title>Underutilised green leafy vegetable (uglv): an overview</title>
                <p>UGLVs are a diverse group of plants that have been historically overlooked in mainstream agriculture but are now gaining attention for their nutritional and medicinal properties. These vegetables are rich in essential nutrients and bioactive compounds, making them valuable for enhancing dietary diversity and addressing nutritional deficiencies in the diet. Many green, leafy vegetables are important for rural folk, as they are a major part of their diet and nutrition. These vegetables, once held in high esteem for their nutritional and medicinal benefits, are now underutilized and unexplored due to changing dietary preferences, lack of awareness, and modern agricultural practices favouring commercially popular crops. The present study aimed to compare the growth and yield performance of different underutilized green leafy vegetables. The growth performance analysis revealed the maximum plant height, leaf breadth, number of leaves per plant, leaf length, leaf area, and yield in different vegetable varieties. Centella asiatica (L.) Urb. is an important medicinal herb and a nutraceutical herb that accumulates highly pentacyclic triterpenoid saponins, an active compound that induces cell rejuvenation to promote physical and mental health (
                    <xref ref-type="bibr" rid="ref7">Bhavithra et al., 2019</xref>). Other purposes for which Centella asiatica is used include being a green leafy vegetable for juice, drinks, and food preparation. Ipomoea aquatica Forsk. is a common green leafy vegetable grown throughout Southeast Asia. It is a rich source of vitamins, minerals, proteins, fiber, carotenoids, and flavonoids, which bestow various health benefits (
                    <xref ref-type="bibr" rid="ref21">Joshi &amp; Chaturvedi, 2013</xref>). Although it is a common weed in lakes and freshwater ponds, it is often overlooked because of the lack of knowledge about this green leafy vegetable. Chench (Corchorus acutangulus Lam.) is an unexploited and underutilized leafy vegetable in India (
                    <xref ref-type="bibr" rid="ref30">Kurrey et al., 2018</xref>). It is a good source of nutrients, including water, energy, protein, fat, carbohydrates, fiber, calcium, phosphorus, iron, and ascorbic acid. The yummiest bitterness in Corchorus leaves is due to the presence of Corchorin glycosides. The growth of green leafy vegetables is highly dependent on several influencing contextual factors, such as the locality's environmental features, climatic conditions, and agronomic activities conducted in that area (
                    <xref ref-type="bibr" rid="ref30">Kurrey et al., 2018</xref>). A concept or logic model should be designed for the digital information management of green leafy vegetable production, allowing for the implementation of information management systems, including the safety monitoring of green leafy vegetables through barcodes (
                    <xref ref-type="bibr" rid="ref61">Shen et al., 2007</xref>).</p>
                <p>Bakery products can be markedly improved in their mineral and fiber contents by using green leafy vegetables such as spinach, chard, and kale. For example, the mineral content has been enhanced by spinach and chard, while the fiber content has been enhanced with other vegetables, such as onions and pak choi (
                    <xref ref-type="bibr" rid="ref52">Santamaria et al., 2023)</xref>. Various studies have focused on developing value-added products using these underutilized leaves (
                    <xref ref-type="bibr" rid="ref22">Joshi &amp; Mathur, 2015</xref>; 
                    <xref ref-type="bibr" rid="ref25">Khatoniar et al., 2018</xref>). Although the integration of these underutilized grains and their ingredients shows great promise, consumers must still accept these products, and consistency in product quality must be assured. Therefore, further research should focus on the formulation and processing of novel bakery solutions.</p>
            </sec>
        </sec>
        <sec id="sec9">
            <title>Methodology</title>
            <p>A systematic literature review was conducted to evaluate the nutritional and organoleptic applications of underutilized green leafy vegetables (GLVs) in bakery products. Four major databases were used to search the literature: Web of Science, Scopus, PubMed, and Google Scholar. There were no language restrictions on this search to ensure a thorough review of the published papers.</p>
            <p>The search strategy involved different combinations of Medical Subject Headings (MeSH) terms to optimize the search results for relevant studies. The search terms include &#x201c;impact&#x201d; OR &#x201c;nutritional use&#x201d; AND &#x201c;organoleptic use&#x201d; AND &#x201c;nutritional and organoleptic use&#x201d; AND &#x201c;impact on nutritional and organoleptic use&#x201d; AND &#x201c;underutilized green leafy vegetables&#x201d; AND &#x201c;GLV&#x201d; AND &#x201c;use of GLV in bakery products.&#x201d; These terms were then combined in various combinations to identify research studies in which GLVs were incorporated into bakery formulations, and their effects on nutritional composition, sensory attributes, and consumer acceptance were evaluated.</p>
            <p>To guarantee the reliability and transparency of the review process, the protocol of this study was registered in the Open Science Framework (OSF); DOI: 
                <ext-link ext-link-type="uri" xlink:href="https://doi.org/10.17605/OSF.IO/7YHDZ">https://doi.org/10.17605/OSF.IO/7YHDZ</ext-link> (
                <xref ref-type="bibr" rid="ref55">SANTHANAM et al., 2026c</xref>). This review follows the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) 2020 and PRISMA 2020 for abstract checklists, which can be accessed on the open-access repository: Figshare at 
                <ext-link ext-link-type="uri" xlink:href="https://doi.org/10.6084/m9.figshare.31878151">https://doi.org/10.6084/m9.figshare.31878151</ext-link> (
                <xref ref-type="bibr" rid="ref57">Santhanam et al., 2026e</xref>). The table below contains further details on the search strategies, including database-specific queries (
                <xref ref-type="table" rid="T1">
Table 1</xref>) and inclusion criteria and exclusion criteria (
                <xref ref-type="table" rid="T2">
Table 2</xref>).</p>
            <table-wrap id="T1" orientation="portrait" position="float">
                <label>
Table 1. </label>
                <caption>
                    <title>Overview of the search strategy and the count of publications across each electronic database.</title>
                </caption>
                <table content-type="article-table" frame="hsides">
                    <thead>
                        <tr>
                            <th align="left" colspan="1" rowspan="1" valign="top">Database</th>
                            <th align="left" colspan="1" rowspan="1" valign="top">Search Strategy</th>
                            <th align="left" colspan="1" rowspan="1" valign="top">No.of Publications</th>
                        </tr>
                    </thead>
                    <tbody>
                        <tr>
                            <td align="left" colspan="1" rowspan="1" valign="top">PubMed</td>
                            <td align="left" colspan="1" rowspan="1" valign="top">Review&#x201d;[TITLE/ABSTRACT]) AND (&#x201c;Food Application&#x201d;[TITLE/ABSTRACT]) AND (&#x201c;Novel Food Application&#x201d;[TITLE/ABSTRACT])</td>
                            <td align="left" colspan="1" rowspan="1" valign="top">1706</td>
                        </tr>
                        <tr>
                            <td align="left" colspan="1" rowspan="1" valign="top">Scopus</td>
                            <td align="left" colspan="1" rowspan="1" valign="top">(&#x201c;Underutilized green leafy vegetables&#x201d;) AND TITLE-ABS-KEY (&#x201c;GLV&#x201d;) AND TITLE-ABS-KEY (&#x201c;Impact on nutritional and organoleptic use&#x201d;) AND TITLE-ABS-KEY (&#x201c;Use of GLV in bakery products&#x201d;) OR TITLE-ABS-KEY (&#x201c;Food Application&#x201d;) AND TITLE-ABS-KEY (&#x201c;Novel Food Application&#x201d;)</td>
                            <td align="left" colspan="1" rowspan="1" valign="top">580</td>
                        </tr>
                        <tr>
                            <td align="left" colspan="1" rowspan="1" valign="top">Web of Science</td>
                            <td align="left" colspan="1" rowspan="1" valign="top">TS&#x00a0;=&#x00a0;(&#x201c;Nutritional use&#x201d; OR &#x201c;Organoleptic use&#x201d; OR &#x201c;Nutritional and organoleptic use&#x201d; OR &#x201c;Impact on nutritional and organoleptic use&#x201d;) AND TS&#x00a0;=&#x00a0;(&#x201c;Underutilized green leafy vegetables&#x201d; OR &#x201c;GLV&#x201d;) AND &#x201c;Use of GLV in bakery products&#x201d;) AND (&#x201c;Novel food application&#x201d;)</td>
                            <td align="left" colspan="1" rowspan="1" valign="top">1109</td>
                        </tr>
                        <tr>
                            <td align="left" colspan="1" rowspan="1" valign="top">Google Scholar</td>
                            <td align="left" colspan="1" rowspan="1" valign="top">Allintitle (&#x201c;Underutilized green leafy vegetables&#x201d; OR &#x201c;GLV&#x201d;) AND (&#x201c;Impact on nutritional and organoleptic use&#x201d;) AND (&#x201c;Use of GLV in bakery products&#x201d;) AND (&#x201c;Food application&#x201d;) AND (&#x201c;Novel food application&#x201d;) AND (&#x201c;Nutritional enhancement&#x201d;) AND (&#x201c;Sensory Acceptability) AND (&#x201c;Fortification&#x201d;)</td>
                            <td align="left" colspan="1" rowspan="1" valign="top">5024</td>
                        </tr>
                    </tbody>
                </table>
            </table-wrap>
            <table-wrap id="T2" orientation="portrait" position="float">
                <label>
Table 2. </label>
                <caption>
                    <title>Criteria for inclusion and exclusion of studies.</title>
                </caption>
                <table content-type="article-table" frame="hsides">
                    <thead>
                        <tr>
                            <th align="left" colspan="1" rowspan="1" valign="top">Inclusion criteria</th>
                            <th align="left" colspan="1" rowspan="1" valign="top">Exclusion criteria</th>
                        </tr>
                    </thead>
                    <tbody>
                        <tr>
                            <td align="left" colspan="1" rowspan="1" valign="top">Review articles, meta-analyses, and systematic reviews relevant to the topic.</td>
                            <td align="left" colspan="1" rowspan="1" valign="top">Studies lacking experimental data or proper control groups.</td>
                        </tr>
                        <tr>
                            <td align="left" colspan="1" rowspan="1" valign="top">Studies that evaluate the nutritional and organoleptic properties of underutilized green leafy vegetables (GLVs).</td>
                            <td align="left" colspan="1" rowspan="1" valign="top">Research not specifically focused on underutilized GLVs in bakery products.</td>
                        </tr>
                        <tr>
                            <td align="left" colspan="1" rowspan="1" valign="top">Studies examining the impact of GLVs on the nutritional profile, sensory attributes (color, texture, taste, aroma), and functional properties of bakery products.</td>
                            <td align="left" colspan="1" rowspan="1" valign="top">Studies assessing consumer acceptability, sensory evaluation scores, and shelf-life improvement.</td>
                        </tr>
                        <tr>
                            <td align="left" colspan="1" rowspan="1" valign="top">Reports on changes in nutritional composition (protein, fiber, vitamins, minerals, bioactive compounds) in bakery products with GLVs.</td>
                            <td align="left" colspan="1" rowspan="1" valign="top">Studies not reporting nutritional analysis or sensory evaluation results.</td>
                        </tr>
                        <tr>
                            <td align="left" colspan="1" rowspan="1" valign="top">Last ten years articles were included for conducting systematic review.</td>
                            <td align="left" colspan="1" rowspan="1" valign="top">Articles published before ten years are excluded.</td>
                        </tr>
                    </tbody>
                </table>
            </table-wrap>
        </sec>
        <sec id="sec10" sec-type="results">
            <title>Results</title>
            <p>The current systematic review follows the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) statement in reporting for systematic reviews. The study selection process is illustrated in the PRISMA flow diagram (
                <xref ref-type="fig" rid="f1">
Figure 1</xref>). A search of four electronic databases (PubMed, Scopus, Web of Science, and Google Scholar) yielded a total of 8419 studies. After removing 752 duplicate records, 877 articles were subjected to title and abstract screening. Seventy-four full-text articles were assessed for eligibility, and 50 were excluded because of the lack of experimental data, absence of appropriate control groups, or irrelevance to underutilized green leafy vegetables (GLVs) in bakery products. Finally, 24 studies were included in the review (
                <xref ref-type="bibr" rid="ref19">Gupta et al., 2005</xref>; 
                <xref ref-type="bibr" rid="ref59">Sarkar et al., 2023</xref>; 
                <xref ref-type="bibr" rid="ref40">Okunlola et al., 2023</xref>; 
                <xref ref-type="bibr" rid="ref50">Yadav &amp; Singh, 2024</xref>; 
                <xref ref-type="bibr" rid="ref7">Bhavithra et al., 2019</xref>; 
                <xref ref-type="bibr" rid="ref32">Marappan et al., 2024</xref>; 
                <xref ref-type="bibr" rid="ref42">Pandiyaraj et al., 2024</xref>; 
                <xref ref-type="bibr" rid="ref22">Joshi &amp; Mathur, 2015</xref>; 
                <xref ref-type="bibr" rid="ref12">Coppock et al., 1952</xref>). PRISMA 2020 flow diagram for systematic review is referred to provide the article screening process (
                <xref ref-type="fig" rid="f1">
Figure 1</xref>).</p>
            <fig fig-type="figure" id="f1" orientation="portrait" position="float">
                <label>
Figure 1. </label>
                <caption>
                    <title>PRISMA 2020 flow diagram illustrating the review process.</title>
                    <p>(n: total count).</p>
                </caption>
                <graphic id="gr1" orientation="portrait" position="float" xlink:href="https://f1000research-files.f1000.com/manuscripts/198722/b4b7fe98-06f7-4b29-a5da-236698b06d08_figure1.gif"/>
            </fig>
            <sec id="sec11">
                <title>Attributes of included studies</title>
                <p>The selection criteria are summarized in 
                    <xref ref-type="table" rid="T3">
Table 3</xref>. Of the 24 selected studies, 15 were published in the last ten years and five were studies examining the impact of GLVs on the nutritional profile and sensory attributes, and four evaluated the nutritional and organoleptic properties of underutilized green leafy vegetables. The studies were conducted in several countries, including India, China, the USA, and other European countries, and were published between 2005 and 2025 (
                    <xref ref-type="bibr" rid="ref19">Gupta et al., 2005</xref>; 
                    <xref ref-type="bibr" rid="ref59">Sarkar et al., 2023</xref>).</p>
                <table-wrap id="T3" orientation="portrait" position="float">
                    <label>
Table 3. </label>
                    <caption>
                        <title>Features of GLV incorporation in bakery products.</title>
                    </caption>
                    <table content-type="article-table" frame="hsides">
                        <thead>
                            <tr>
                                <th align="left" colspan="1" rowspan="1" valign="top">Sr. No.</th>
                                <th align="left" colspan="1" rowspan="1" valign="top">Sub- Heading</th>
                                <th align="left" colspan="1" rowspan="1" valign="top">Content to be Discussed</th>
                                <th align="left" colspan="1" rowspan="1" valign="top">First Author</th>
                                <th align="left" colspan="1" rowspan="1" valign="top">Year</th>
                            </tr>
                        </thead>
                        <tbody>
                            <tr>
                                <td align="left" colspan="1" rowspan="1" valign="top">1</td>
                                <td align="left" colspan="1" rowspan="1" valign="top">Underutilized GLVs</td>
                                <td align="left" colspan="1" rowspan="1" valign="top">Underutilized green leafy vegetables (GLVs) are often neglected due to their perception as weeds or inferior plants. However, they possess significant health benefits and can be effectively utilized for food fortification.</td>
                                <td align="left" colspan="1" rowspan="1" valign="top">Gupta et al.</td>
                                <td align="left" colspan="1" rowspan="1" valign="top">
                                    <xref ref-type="bibr" rid="ref19">2005</xref>
                                </td>
                            </tr>
                            <tr>
                                <td align="left" colspan="1" rowspan="1" valign="top">2</td>
                                <td align="left" colspan="1" rowspan="1" valign="top">Nutritional Composition</td>
                                <td align="left" colspan="1" rowspan="1" valign="top">Underutilized GLVs are rich in essential nutrients, including minerals (iron, calcium, zinc), vitamins (beta-carotene, vitamin C, and E), and phytochemicals such as dietary fiber, phenols, and antioxidants.</td>
                                <td align="left" colspan="1" rowspan="1" valign="top">Sarkar et al.</td>
                                <td align="left" colspan="1" rowspan="1" valign="top">
                                    <xref ref-type="bibr" rid="ref59">2023</xref>
                                </td>
                            </tr>
                            <tr>
                                <td align="left" colspan="1" rowspan="1" valign="top">3</td>
                                <td align="left" colspan="1" rowspan="1" valign="top">Health Benefits and Medicinal Properties</td>
                                <td align="left" colspan="1" rowspan="1" valign="top">GLVs contain bioactive compounds that contribute to their medicinal properties, supporting nutritional requirements and providing protection against diseases associated with malnutrition.</td>
                                <td align="left" colspan="1" rowspan="1" valign="top">Okunlola et al.</td>
                                <td align="left" colspan="1" rowspan="1" valign="top">
                                    <xref ref-type="bibr" rid="ref40">2023</xref>
                                </td>
                            </tr>
                            <tr>
                                <td align="left" colspan="1" rowspan="1" valign="top">4</td>
                                <td align="left" colspan="1" rowspan="1" valign="top">Food Fortification Potential</td>
                                <td align="left" colspan="1" rowspan="1" valign="top">Underutilized GLVs have strong potential as food fortificants due to their rich nutritional and bioactive profiles. Processing methods play a crucial role in maintaining their nutritional quality for commercial applications.</td>
                                <td align="left" colspan="1" rowspan="1" valign="top">Yadav &amp; Singh</td>
                                <td align="left" colspan="1" rowspan="1" valign="top">
                                    <xref ref-type="bibr" rid="ref50">2024</xref>
                                </td>
                            </tr>
                            <tr>
                                <td align="left" colspan="1" rowspan="1" valign="top">5</td>
                                <td align="left" colspan="1" rowspan="1" valign="top">Challenges and Future Directions</td>
                                <td align="left" colspan="1" rowspan="1" valign="top">Underutilized GLVs have strong potential as food fortificants due to their rich nutritional and bioactive profiles. Processing methods play a crucial role in maintaining their nutritional quality for commercial applications.</td>
                                <td align="left" colspan="1" rowspan="1" valign="top">Bhavithra et al.</td>
                                <td align="left" colspan="1" rowspan="1" valign="top">
                                    <xref ref-type="bibr" rid="ref7">2019</xref>
                                </td>
                            </tr>
                            <tr>
                                <td align="left" colspan="1" rowspan="1" valign="top">6</td>
                                <td align="left" colspan="1" rowspan="1" valign="top">Nutritional Value</td>
                                <td align="left" colspan="1" rowspan="1" valign="top">GLVs are high in vitamins A, C, and E, as well as important minerals such as potassium and calcium. Some varieties such as cauliflower and carrot leaves have significant protein levels.</td>
                                <td align="left" colspan="1" rowspan="1" valign="top">Marappan et al.</td>
                                <td align="left" colspan="1" rowspan="1" valign="top">
                                    <xref ref-type="bibr" rid="ref32">2024</xref>
                                </td>
                            </tr>
                            <tr>
                                <td align="left" colspan="1" rowspan="1" valign="top">7</td>
                                <td align="left" colspan="1" rowspan="1" valign="top">Economic and Agricultural Impact</td>
                                <td align="left" colspan="1" rowspan="1" valign="top">Cultivation of GLVs can enhance agricultural biodiversity and provide economic benefits to smallholder farmers by reducing dependence on conventional crops.</td>
                                <td align="left" colspan="1" rowspan="1" valign="top">Pandiyaraj et al.</td>
                                <td align="left" colspan="1" rowspan="1" valign="top">
                                    <xref ref-type="bibr" rid="ref42">2024</xref>
                                </td>
                            </tr>
                            <tr>
                                <td align="left" colspan="1" rowspan="1" valign="top">8</td>
                                <td align="left" colspan="1" rowspan="1" valign="top">Nutritional Benefits of Underutilized GLVs in Bakery Products</td>
                                <td align="left" colspan="1" rowspan="1" valign="top">GLVs incorporated into bakery products enhance micronutrient content, including iron, calcium, zinc, and vitamins such as beta-carotene, vitamin E, vitamin K, and vitamin C, contributing to improved health outcomes.</td>
                                <td align="left" colspan="1" rowspan="1" valign="top">Sarkar et al.</td>
                                <td align="left" colspan="1" rowspan="1" valign="top">
                                    <xref ref-type="bibr" rid="ref59">2023</xref>
                                </td>
                            </tr>
                            <tr>
                                <td align="left" colspan="1" rowspan="1" valign="top">9</td>
                                <td align="left" colspan="1" rowspan="1" valign="top">Application in Bakery Products</td>
                                <td align="left" colspan="1" rowspan="1" valign="top">Dehydrated leaf powders from GLVs can be incorporated into bakery products to improve their nutritional profile, particularly through increased micronutrient content.</td>
                                <td align="left" colspan="1" rowspan="1" valign="top">Joshi &amp; Mathur</td>
                                <td align="left" colspan="1" rowspan="1" valign="top">
                                    <xref ref-type="bibr" rid="ref22">2015</xref>
                                </td>
                            </tr>
                            <tr>
                                <td align="left" colspan="1" rowspan="1" valign="top">10</td>
                                <td align="left" colspan="1" rowspan="1" valign="top">Organoleptic Properties of Bakery Products Containing GLVs</td>
                                <td align="left" colspan="1" rowspan="1" valign="top">Sensory attributes such as taste, texture, and overall quality significantly influence consumer acceptance of bakery products. Factors such as flour type, shortening, and additives also affect organoleptic properties.</td>
                                <td align="left" colspan="1" rowspan="1" valign="top">Coppock et al.</td>
                                <td align="left" colspan="1" rowspan="1" valign="top">
                                    <xref ref-type="bibr" rid="ref12">1952</xref>
                                </td>
                            </tr>
                        </tbody>
                    </table>
                </table-wrap>
            </sec>
            <sec id="sec12">
                <title>Attributes of excluded studies</title>
                <p>Studies that did not present experimental data were excluded as a criterion by which the merit of the study findings could be judged. Furthermore, studies lacking proper control groups were excluded to allow reliable comparisons between test and control samples. Participants who considered underutilized green leafy vegetables but had no specific inclinations about their application in bakery products were also excluded. Furthermore, studies that did not include any nutritional analysis or sensory evaluation were excluded, as these were essential to show the effect of GLVs on bakery formulations. Articles published before ten years were excluded. There were 50 studies rejected on these grounds.</p>
            </sec>
            <sec id="sec13">
                <title>Advancement in nutrition bioactive component</title>
                <p>The incorporation of underutilized green leafy vegetables (GLVs) into baked goods significantly increased the levels of dietary fiber, vitamins (A, C, E, K, and B-complex), minerals (iron, calcium, and zinc), and bioactive compounds (polyphenols, flavonoids, and carotenoids). Several studies have reported that the addition of GLVs enhances the antioxidant properties of bakery products, thereby contributing to their functional value. For instance, GLV-enriched bakery products exhibited 30&#x2013;50% higher total phenolic content and antioxidant activity compared to their conventional counterparts (
                    <xref ref-type="bibr" rid="ref59">Sarkar et al., 2023</xref>).</p>
                <p>

                    <bold>Assessment of Sensory Context and Consumer Perception:</bold> Although improvements in nutritional quality have been observed, sensory attributes such as bitterness, color, and texture remain critical determinants of consumer acceptability. The overall mean acceptability scores across six studies ranged from 6.5 to 8.2 on a 10-point hedonic scale. To address these sensory challenges, various studies have recommended processing techniques such as dehydration and fermentation to reduce undesirable characteristics, as supported by 
                    <xref ref-type="bibr" rid="ref12">Coppock et al. (1952)</xref> and 
                    <xref ref-type="bibr" rid="ref68">Naqash et al. (2017)</xref>.</p>
            </sec>
            <sec id="sec14">
                <title>Challenges of technical feasibility and processing</title>
                <p>The major technological challenges associated with the incorporation of underutilized green leafy vegetables (GLVs) into bakery formulations include alterations in dough rheology, texture, and shelf-life stability. The addition of GLV powders influences moisture absorption behavior, thereby affecting dough elasticity and crumb structure. Processing techniques such as fermentation and controlled oxidative treatments can improve texture and palatability. Additionally, drying methods, including hot air drying and freeze drying, are effective in preserving bioactive compounds while maintaining desirable sensory attributes. These technological observations have been reported by 
                    <xref ref-type="bibr" rid="ref4">Belokurova et al. (2015)</xref> and 
                    <xref ref-type="bibr" rid="ref34">Menaka et al. (2024)</xref>.</p>
            </sec>
            <sec id="sec15">
                <title>Study quality evaluation and limitations</title>
                <p>Quality assessment based on the Newcastle-Ottawa Scale (NOS) showed that the included studies had scores ranging between 6 and 9, most being medium to high grade. However, heterogeneity in study designs, GLV types, processing methods, and analytic techniques precluded a feasible quantitative synthesis or meta-analysis.</p>
                <p>In summary, according to 10 out of 24 studies, the incorporation of GLVs improved the nutritional and functional properties of bakery products, while 14 did not show a significant difference. Owing to the different methodologies and experimental protocols, complete quantitative synthesis is not possible.</p>
            </sec>
        </sec>
        <sec id="sec16" sec-type="discussion">
            <title>Discussion</title>
            <p>Integrating green leafy vegetables (GLVs) into baked goods is a promising strategy to enhance the nutritional value and sustainability of processed foods. Most GLVs have good nutrient compositions, but they remain underutilized because of perishability, sensory difficulties, and lack of consumer knowledge. This discussion will explore facilitation of the incorporation of GLVs into baked products along with the technological, nutritional, and functional aspects of GLVs in bakery products and the challenges and opportunities that arise with them. Advanced technologies, such as ingredient substitution and process optimization, will make GLV incorporation healthy and viable while maintaining the structural integrity of the preferred bakery product. In addition, new applications concerning largely ignored GLVs and traditional grains may resolve sustainability issues in the development of new products. The functional application of these ingredients provides antioxidant capacity and improved product stability. However, emerging barriers to perishability, formulation, and market acceptance should be addressed using innovative processing technologies and consumer education.</p>
            <p>The 
                <xref ref-type="table" rid="T3">table 3</xref> and 
                <xref ref-type="table" rid="T4">4</xref> present the broad features of GLV incorporation in bakery products, including technological, health, and possible obstacles in upscaling applications. Technical challenges include dough rheology, texture, and shelf-life stability issues. Fermentation and drying processes can improve the texture and maintain the bioactive compounds.</p>
            <table-wrap id="T4" orientation="portrait" position="float">
                <label>
Table 4. </label>
                <caption>
                    <title>Features of GLV incorporation in bakery products.</title>
                </caption>
                <table content-type="article-table" frame="hsides">
                    <thead>
                        <tr>
                            <th align="left" colspan="1" rowspan="1" valign="top">Sr. No.</th>
                            <th align="left" colspan="1" rowspan="1" valign="top">Sub- Heading</th>
                            <th align="left" colspan="1" rowspan="1" valign="top">Content to be Discussed</th>
                            <th align="left" colspan="1" rowspan="1" valign="top">First Author</th>
                            <th align="left" colspan="1" rowspan="1" valign="top">Year</th>
                        </tr>
                    </thead>
                    <tbody>
                        <tr>
                            <td align="left" colspan="1" rowspan="1" valign="top">01</td>
                            <td align="left" colspan="1" rowspan="1" valign="top">Technological Aspects and Challenges in Incorporating GLVs into Bakery Products</td>
                            <td align="left" colspan="1" rowspan="1" valign="top">Incorporation of GLVs affects dough rheology, texture, and shelf-life stability. Challenges include interference with fermentation, optimization of processing conditions, and increased production costs. Gluten-free formulations further complicate product development due to the absence of gluten structure.</td>
                            <td align="left" colspan="1" rowspan="1" valign="top">Belokurova et al.</td>
                            <td align="left" colspan="1" rowspan="1" valign="top">
                                <xref ref-type="bibr" rid="ref4">2015</xref>
                            </td>
                        </tr>
                        <tr>
                            <td colspan="1" rowspan="1"/>
                            <td align="left" colspan="1" rowspan="1" valign="top">Technological Approaches</td>
                            <td align="left" colspan="1" rowspan="1" valign="top">Approaches include gluten-free flour substitution, process optimization, and fermentation techniques such as sourdough fermentation. Advanced processing methods improve product quality and stability.</td>
                            <td align="left" colspan="1" rowspan="1" valign="top">Naqash et al., Menaka et al.</td>
                            <td align="left" colspan="1" rowspan="1" valign="top">
                                <xref ref-type="bibr" rid="ref68">2017</xref>, 
                                <xref ref-type="bibr" rid="ref34">2024</xref>
                            </td>
                        </tr>
                        <tr>
                            <td colspan="1" rowspan="1"/>
                            <td align="left" colspan="1" rowspan="1" valign="top">Nutritional Enhancement</td>
                            <td align="left" colspan="1" rowspan="1" valign="top">GLVs enhance mineral and fiber content, while vegetable powders contribute antioxidants, vitamins, and bioactive compounds. Functional ingredients and by-products improve sustainability and nutritional quality.</td>
                            <td align="left" colspan="1" rowspan="1" valign="top">Santamaria et al.; Salehi et al.</td>
                            <td align="left" colspan="1" rowspan="1" valign="top">
                                <xref ref-type="bibr" rid="ref52">2023</xref>; 
                                <xref ref-type="bibr" rid="ref69">2019</xref>
                            </td>
                        </tr>
                        <tr>
                            <td colspan="1" rowspan="1"/>
                            <td align="left" colspan="1" rowspan="1" valign="top">Challenges in Quality</td>
                            <td align="left" colspan="1" rowspan="1" valign="top">Issues such as altered texture, volume, and color arise due to gluten absence and incorporation of vegetable flours. Rheological changes affect dough consistency and final product quality.</td>
                            <td align="left" colspan="1" rowspan="1" valign="top">Alashbayeva et al.; Gallardo et al.</td>
                            <td align="left" colspan="1" rowspan="1" valign="top">
                                <xref ref-type="bibr" rid="ref1">2024</xref>
                            </td>
                        </tr>
                        <tr>
                            <td align="left" colspan="1" rowspan="1" valign="top">02</td>
                            <td align="left" colspan="1" rowspan="1" valign="top">Novel Applications of Underutilized GLVs in Bakery Products</td>
                            <td align="left" colspan="1" rowspan="1" valign="top">Underutilized leaves such as beetroot, carrot, and cauliflower are incorporated to improve nutritional quality. Lactic acid bacteria enhance shelf life and functionality, while fiber concentrates increase antioxidant properties.</td>
                            <td align="left" colspan="1" rowspan="1" valign="top">Joshi &amp; Mathur; Liu et al.</td>
                            <td align="left" colspan="1" rowspan="1" valign="top">
                                <xref ref-type="bibr" rid="ref22">2015</xref>, 
                                <xref ref-type="bibr" rid="ref31">2022</xref>
                            </td>
                        </tr>
                        <tr>
                            <td colspan="1" rowspan="1"/>
                            <td align="left" colspan="1" rowspan="1" valign="top">Ancient Grains in Bakery Products</td>
                            <td align="left" colspan="1" rowspan="1" valign="top">Ancient grains such as spelt and einkorn improve nutritional value but require process optimization. Hydrocolloid use and hydration control enhance baking performance.</td>
                            <td align="left" colspan="1" rowspan="1" valign="top">Valsalan et al.</td>
                            <td align="left" colspan="1" rowspan="1" valign="top">
                                <xref ref-type="bibr" rid="ref66">2024</xref>
                            </td>
                        </tr>
                        <tr>
                            <td colspan="1" rowspan="1"/>
                            <td align="left" colspan="1" rowspan="1" valign="top">Nutritional Enhancement</td>
                            <td align="left" colspan="1" rowspan="1" valign="top">Incorporation of GLVs such as broccoli leaves increases antioxidant potential and micronutrient content. Protein and fiber enrichment aligns with consumer health trends.</td>
                            <td align="left" colspan="1" rowspan="1" valign="top">Krupa-Kozak et al., P. Kumar et al., Cetiner et al.</td>
                            <td align="left" colspan="1" rowspan="1" valign="top">
                                <xref ref-type="bibr" rid="ref26">2021</xref>; 
                                <xref ref-type="bibr" rid="ref27">2022</xref>; 
                                <xref ref-type="bibr" rid="ref9">2023</xref>
                            </td>
                        </tr>
                        <tr>
                            <td colspan="1" rowspan="1"/>
                            <td align="left" colspan="1" rowspan="1" valign="top">Functional Properties</td>
                            <td align="left" colspan="1" rowspan="1" valign="top">GLVs improve functional properties through bioactive compounds, enhanced digestibility, and health-promoting effects such as antioxidant and anti-glycation activity.</td>
                            <td align="left" colspan="1" rowspan="1" valign="top">Krupa-Kozak et al.</td>
                            <td align="left" colspan="1" rowspan="1" valign="top">
                                <xref ref-type="bibr" rid="ref26">2021</xref>
                            </td>
                        </tr>
                        <tr>
                            <td colspan="1" rowspan="1"/>
                            <td align="left" colspan="1" rowspan="1" valign="top">Sustainability and Waste Reduction</td>
                            <td align="left" colspan="1" rowspan="1" valign="top">Use of vegetable by-products supports sustainability by reducing food waste. Revalorization techniques enhance resource efficiency in food systems.</td>
                            <td align="left" colspan="1" rowspan="1" valign="top">Martinez &amp; G&#x00f3;mez; Cano-Lamadrid et al</td>
                            <td align="left" colspan="1" rowspan="1" valign="top">
                                <xref ref-type="bibr" rid="ref33">2024</xref>; 
                                <xref ref-type="bibr" rid="ref8">2022</xref>
                            </td>
                        </tr>
                        <tr>
                            <td align="left" colspan="1" rowspan="1" valign="top">03</td>
                            <td align="left" colspan="1" rowspan="1" valign="top">Health Implications and Functional Benefits of Bakery Products with GLVs</td>
                            <td align="left" colspan="1" rowspan="1" valign="top">GLVs provide essential nutrients including fiber, vitamins, and minerals, contributing to the prevention of chronic diseases and micronutrient deficiencies. Limited awareness restricts their widespread consumption.</td>
                            <td align="left" colspan="1" rowspan="1" valign="top"/>
                            <td align="left" colspan="1" rowspan="1" valign="top"/>
                        </tr>
                        <tr>
                            <td colspan="1" rowspan="1"/>
                            <td align="left" colspan="1" rowspan="1" valign="top">Nutritional Benefits</td>
                            <td align="left" colspan="1" rowspan="1" valign="top">GLVs such as red spinach and amaranth are rich in essential nutrients. Cauliflower leaves contribute significant protein content in bakery formulations.</td>
                            <td align="left" colspan="1" rowspan="1" valign="top">Sarkar et al., Yadav &amp; Singh</td>
                            <td align="left" colspan="1" rowspan="1" valign="top">
                                <xref ref-type="bibr" rid="ref59">2023</xref>; 
                                <xref ref-type="bibr" rid="ref50">2024</xref>
                            </td>
                        </tr>
                        <tr>
                            <td colspan="1" rowspan="1"/>
                            <td align="left" colspan="1" rowspan="1" valign="top">Functional Properties</td>
                            <td align="left" colspan="1" rowspan="1" valign="top">High phenolic content in GLVs helps reduce oxidative stress. Increased dietary fiber improves digestion and satiety.</td>
                            <td align="left" colspan="1" rowspan="1" valign="top">Sarkar et al.</td>
                            <td align="left" colspan="1" rowspan="1" valign="top">
                                <xref ref-type="bibr" rid="ref59">2023</xref>
                            </td>
                        </tr>
                        <tr>
                            <td colspan="1" rowspan="1"/>
                            <td align="left" colspan="1" rowspan="1" valign="top">Health Implications</td>
                            <td align="left" colspan="1" rowspan="1" valign="top">Regular consumption of GLVs reduces risks of chronic diseases and supports functional food development due to bioactive compounds.</td>
                            <td align="left" colspan="1" rowspan="1" valign="top">Sarkar et al.</td>
                            <td align="left" colspan="1" rowspan="1" valign="top">
                                <xref ref-type="bibr" rid="ref59">2023</xref>
                            </td>
                        </tr>
                        <tr>
                            <td align="left" colspan="1" rowspan="1" valign="top">04</td>
                            <td align="left" colspan="1" rowspan="1" valign="top">Barriers and Opportunities for Scaling Up Green Leafy Vegetable
                                <break/>Use in Bakery Products</td>
                            <td align="left" colspan="1" rowspan="1" valign="top">Challenges include perishability, changes in dough rheology, and limited consumer acceptance. Opportunities include dehydration, fortification, and innovative product development.</td>
                            <td align="left" colspan="1" rowspan="1" valign="top">Khatoniar et al.; Liu et al.</td>
                            <td align="left" colspan="1" rowspan="1" valign="top">
                                <xref ref-type="bibr" rid="ref25">2018</xref>; 
                                <xref ref-type="bibr" rid="ref31">2022</xref>
                            </td>
                        </tr>
                        <tr>
                            <td colspan="1" rowspan="1"/>
                            <td align="left" colspan="1" rowspan="1" valign="top">Barriers to Scaling Up GLV Use</td>
                            <td align="left" colspan="1" rowspan="1" valign="top">High moisture content leads to spoilage, while changes in dough resistance and extensibility affect product quality.</td>
                            <td align="left" colspan="1" rowspan="1" valign="top">Naumenko et al.</td>
                            <td align="left" colspan="1" rowspan="1" valign="top">
                                <xref ref-type="bibr" rid="ref37">2024</xref>
                            </td>
                        </tr>
                        <tr>
                            <td colspan="1" rowspan="1"/>
                            <td align="left" colspan="1" rowspan="1" valign="top">Opportunities for Scaling Up GLV Use</td>
                            <td align="left" colspan="1" rowspan="1" valign="top">Dehydration improves shelf life, while fortification enhances nutritional value. Research-driven product development supports innovation in bakery applications.</td>
                            <td align="left" colspan="1" rowspan="1" valign="top">Khatoniar et al.; Osipova et al.</td>
                            <td align="left" colspan="1" rowspan="1" valign="top">
                                <xref ref-type="bibr" rid="ref25">2018</xref>, 
                                <xref ref-type="bibr" rid="ref41">2020</xref>
                            </td>
                        </tr>
                    </tbody>
                </table>
            </table-wrap>
            <p>Baked products can be complemented with green leafy vegetables (GLVs) to enhance nutrition and environmental sustainability. GLVs are used sparingly because of their perishability, lack of sensory appeal, and poor consumer awareness. Modern technologies will allow the incorporation of GLVs into products while maintaining their original attributes. Tackling perishability and market acceptability is vital for successful product development.</p>
            <sec id="sec17">
                <title>Strengths and limitations</title>
                <p>This systematic review was conducted according to the PRISMA guidelines. It followed comprehensive and systematic procedures for the selection and analysis of the literature. Research from four major databases (PubMed, Scopus, Web of Science, and Google Scholar) was included in this study, regardless of the language. This negates the possibility of a publication bias. This synthesis evaluates the nutritional and sensory aspects of the improvements brought by incorporating underutilized green leafy vegetables into baked products, thereby functioning as functional foods. The findings will also contribute to the emerging field of sustainable nutrition by positing underutilized GLVs as contributors to greater dietary diversity.</p>
                <p>However, this review has some limitations. Most of the studies included were either observational or experimental, making it difficult to establish a direct causation between GLV incorporation and direct health outcomes. Diverse designs, processing methods, and analytic techniques did not permit a meta-analysis in this study. In addition, biases could arise from differences in sensory evaluation methods and consumer acceptability assessments, as these are mainly self-reported. However, it does not investigate the long-term stability or commercial acceptability of GLV-laden bakery products. Future studies must standardize the formulas and employ randomized controlled trials for functional and sensory validation in real-life implementation contexts.</p>
            </sec>
        </sec>
        <sec id="sec18" sec-type="conclusions">
            <title>Conclusions</title>
            <p>A systematic review of the effects of underutilized green leafy vegetables (GLVs) in bakery products has drawn several striking conclusions. The nutritional benefits of adding GLVs to baked products include the introduction of vitamins and minerals into baked foods, thereby augmenting dietary diversity and nutritional intake.</p>
            <p>GLVs not only improve nutritional value but also contain bioactive compounds that provide health benefits, such as glycemic control and antioxidant effects. Thus, GLVs can be considered potential functional foods that are beneficial for health.</p>
            <p>Consumer Sensory Challenges: According to the review, nutritional benefits aside, sensory acceptance continues to be a barrier to consumption. Bitterness and texture can both play a role in consumer acceptance. In addition, the threshold of inclusion of GLVs varies according to the specific GLV and processing methods.</p>
            <p>

                <bold>Technological Solutions:</bold> The review suggests the adoption of technological methods, such as drying and fermentation, to address sensory issues. These techniques will help eliminate unwarranted sensory effects, making the GLV bakery more palatable.</p>
            <p>

                <bold>Need for Standardization:</bold> As observed in this review, future research should be directed toward formulating standardized and randomized controlled trials. In this way, functional and sensory properties can be confirmed in a real-life context, ensuring that consumers are catered to and benefits are maximized.</p>
            <p>

                <bold>Gaps in Knowledge:</bold> The review shows that research shows a wide gap in existing literature, especially regarding studies geared towards establishing direct causa and understanding the activity and contribution of individual GLVs in humans.</p>
        </sec>
    </body>
    <back>
        <sec id="sec21" sec-type="data-availability">
            <title>Data and software availability</title>
            <sec id="sec22">
                <title>Underlying data</title>
                <p>

                    <bold>Figshare</bold>. Data Extraction Table. 
                    <ext-link ext-link-type="uri" xlink:href="https://doi.org/10.6084/m9.figshare.31970022">https://doi.org/10.6084/m9.figshare.31970022</ext-link> (
                    <xref ref-type="bibr" rid="ref53">Santhanam et al., 2026a</xref>).</p>
                <p>Above file contains the following underlying data: Data extraction table. (The screened articles will be organised in the mentioned data sheet.)</p>
                <p>

                    <bold>Figshare</bold>. Search Strategy. 
                    <ext-link ext-link-type="uri" xlink:href="https://doi.org/10.6084/m9.figshare.31970049">https://doi.org/10.6084/m9.figshare.31970049</ext-link> (
                    <xref ref-type="bibr" rid="ref54">Santhanam et al., 2026b</xref>).</p>
                <p>Above file contains the following underlying data: Research study design (The above document includes the study design used for the systematic literature review.)</p>
            </sec>
            <sec id="sec23">
                <title>Extended data</title>
                <p>

                    <bold>Open Science Framework (OSF):</bold> Impact of Nutritional and Organoleptic Use of Underutilized Green Leafy Vegetables in Bakery Products: A Systematic Review of Novel Food Applications; DOI: 
                    <ext-link ext-link-type="uri" xlink:href="https://doi.org/10.17605/OSF.IO/7YHDZ">https://doi.org/10.17605/OSF.IO/7YHDZ</ext-link> (
                    <xref ref-type="bibr" rid="ref55">SANTHANAM et al., 2026c</xref>).</p>
                <p>This project contains the following extended data: OSF project files (Systematic review protocol and related documentation).</p>
                <p>

                    <bold>Figshare</bold>. Data synthesis table. 
                    <ext-link ext-link-type="uri" xlink:href="https://doi.org/10.6084/m9.figshare.31970184">https://doi.org/10.6084/m9.figshare.31970184</ext-link> (
                    <xref ref-type="bibr" rid="ref56">Santhanam et al., 2026d</xref>). This file contains the extended data table of the synthesised articles, which were finalised after screening for eligibility criteria.</p>
            </sec>
            <sec id="sec24">
                <title>Reporting guidelines</title>
                <p>

                    <bold>Figshare</bold>: PRISMA CHECKLIST AND PRISMA ABSTRACT CHECKLIST; DOI: 
                    <ext-link ext-link-type="uri" xlink:href="https://doi.org/10.6084/m9.figshare.31878151">https://doi.org/10.6084/m9.figshare.31878151</ext-link> (
                    <xref ref-type="bibr" rid="ref57">Santhanam et al., 2026e</xref>).</p>
                <p>This project contains the following reporting guidelines: PRISMA checklist.pdf (Checklist followed for systematic review reporting).</p>
                <p>Study data can be accessed according to the terms of the 
                    <ext-link ext-link-type="uri" xlink:href="https://creativecommons.org/licenses/by/4.0/">Creative Commons Attribution 4.0 International License (CC-BY 4.0)</ext-link>.</p>
            </sec>
        </sec>
        <ack>
            <title>Acknowledgements</title>
            <p>The authors would like to express their sincere gratitude to Welcomgroup Graduate School of Hotel Administration and Kasturba Medical College for their invaluable support and resources that contributed to the successful completion of this research. Their guidance and facilities provided a strong foundation for our study, enabling us to conduct a comprehensive investigation into the nutritional and organoleptic properties of underutilized green leafy vegetables in bakery products. We also appreciate all individuals and institutions who assisted us throughout this research.</p>
        </ack>
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    <sub-article article-type="reviewer-report" id="report485439">
        <front-stub>
            <article-id pub-id-type="doi">10.5256/f1000research.198722.r485439</article-id>
            <title-group>
                <article-title>Reviewer response for version 1</article-title>
            </title-group>
            <contrib-group>
                <contrib contrib-type="author">
                    <name>
                        <surname>Dishri</surname>
                        <given-names>Madhusmita</given-names>
                    </name>
                    <xref ref-type="aff" rid="r485439a1">1</xref>
                    <role>Referee</role>
                </contrib>
                <aff id="r485439a1">
                    <label>1</label>Vellore Institute of Technology, Vellore, Tamil Nadu, India</aff>
            </contrib-group>
            <author-notes>
                <fn fn-type="conflict">
                    <p>
                        <bold>Competing interests: </bold>No competing interests were disclosed.</p>
                </fn>
            </author-notes>
            <pub-date pub-type="epub">
                <day>5</day>
                <month>6</month>
                <year>2026</year>
            </pub-date>
            <permissions>
                <copyright-statement>Copyright: &#x00a9; 2026 Dishri M</copyright-statement>
                <copyright-year>2026</copyright-year>
                <license xlink:href="https://creativecommons.org/licenses/by/4.0/">
                    <license-p>This is an open access peer review report distributed under the terms of the Creative Commons Attribution Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.</license-p>
                </license>
            </permissions>
            <related-article ext-link-type="doi" id="relatedArticleReport485439" related-article-type="peer-reviewed-article" xlink:href="10.12688/f1000research.180136.1"/>
            <custom-meta-group>
                <custom-meta>
                    <meta-name>recommendation</meta-name>
                    <meta-value>approve-with-reservations</meta-value>
                </custom-meta>
            </custom-meta-group>
        </front-stub>
        <body>
            <p>The review entitled &#x2018;Impact of nutritional and organoleptic use of underutilized green leafy vegetables in Bakery products: A systematic review of Novel Food applications&#x201d; addressed the role of underutilized green leafy vegetables on nutritional and sensory properties of bakery products. 
                <list list-type="order">
                    <list-item>
                        <p>Researchers studying functional food development and food fortification will find this paper to be a helpful starting point, especially when considering South Asian dietary patterns.</p>
                    </list-item>
                    <list-item>
                        <p>This study address a real gap which says GLVs are nutritionally rich but systematically understudied in processed food applications with focus on sensory/organoleptic results and nutritional fortification is practically beneficial.</p>
                    </list-item>
                    <list-item>
                        <p>Provides transparency and reproducibility by adhering to PRISMA 2020 requirements with a pre-registered procedure and searched databases that reduce publication bias, demonstrated inclusion/exclusion criteria and quality assessment with&#x00a0; Newcastle-Ottawa scale.</p>
                    </list-item>
                    <list-item>
                        <p>However, before it can be regarded as a robust systematic review, a number of methodological issues must be fixed because the conclusions in its current state outweigh the evidence which indicated many studies demonstrated no significant difference.</p>
                    </list-item>
                    <list-item>
                        <p>The idea that sensory acceptability is only difficult to "address" is an understatement; bitterness and texture are well-established obstacles that have prevented commercialisation for decades.</p>
                    </list-item>
                    <list-item>
                        <p>Table 3 contains a verbatim duplicate: the content descriptions of entries 4 ("Food Fortification Potential") and 5 ("Challenges and Future Directions") are identical, indicating a copy-paste error. Further, it lacks meaningful comparison of findings.</p>
                    </list-item>
                    <list-item>
                        <p>The introduction is short, more like narrative instead of summarising the research gaps in the literature.</p>
                    </list-item>
                    <list-item>
                        <p>Gap identification is not explained properly, and there is insufficient data on the trade-offs between nutrition and taste when GLVs are added to baked foods.</p>
                    </list-item>
                </list> 
                <bold>Suggestion</bold> 
                <list list-type="bullet">
                    <list-item>
                        <p>Elaborate rising concern of micronutrient deficiencies and the importance of nutritional diversification</p>
                    </list-item>
                    <list-item>
                        <p>Clear definition of GLVs with their geographical distribution, biodiversity and its nutritional significance, wide consumption and global production of Bakery products, role of functional fortification</p>
                    </list-item>
                    <list-item>
                        <p>Taxonomy of few economically important GLVs species with their botanical description</p>
                    </list-item>
                    <list-item>
                        <p>Comparative tables of proximate analysis micronutrient ,phytochemical and bioactive compound ,antinutritional factors is lacking</p>
                    </list-item>
                    <list-item>
                        <p>Details of pre-processing forms used in baking like&#x00a0; dried , powdered, blanched, fresh and its effect in nutrition retention</p>
                    </list-item>
                    <list-item>
                        <p>Include nutritional impact in Bakery products such as breads, biscuits and cookies, cakes and muffins, crackers and extruded snacks with respects to micronutrients, protein,&#x00a0; effect of baking conditions; temperature and time on micronutrients, glycaemic index, vitamin retention, high temperature effect,&#x00a0; bioavailability considerations,&#x00a0; overall table demonstrating GLV, Bakery product,&#x00a0; key nutritional changes etc .</p>
                    </list-item>
                    <list-item>
                        <p>Outline the organoleptic and sensory properties: colour and appearance, texture and rheology, flavour and aroma, taste and mouth feel, overall acceptability</p>
                    </list-item>
                    <list-item>
                        <p>Methodological comparison across reviewed studies in summary table need to be added</p>
                    </list-item>
                    <list-item>
                        <p>Address the technological and processing. consideration</p>
                    </list-item>
                    <list-item>
                        <p>Elaborate on the discussion stating the reason for the non-significant difference in types of green leafy vegetables, different processing methods and product types. Expand consumer acceptability and commercial feasibility.</p>
                    </list-item>
                </list>
            </p>
            <p>Are the rationale for, and objectives of, the Systematic Review clearly stated?</p>
            <p>Yes</p>
            <p>Is the statistical analysis and its interpretation appropriate?</p>
            <p>Yes</p>
            <p>If this is a Living Systematic Review, is the &#x2018;living&#x2019; method appropriate and is the search schedule clearly defined and justified? (&#x2018;Living Systematic Review&#x2019; or a variation of this term should be included in the title.)</p>
            <p>Not applicable</p>
            <p>Are sufficient details of the methods and analysis provided to allow replication by others?</p>
            <p>Partly</p>
            <p>Are the conclusions drawn adequately supported by the results presented in the review?</p>
            <p>Partly</p>
            <p>Reviewer Expertise:</p>
            <p>Vegetable Science (Horticulture)</p>
            <p>I confirm that I have read this submission and believe that I have an appropriate level of expertise to confirm that it is of an acceptable scientific standard, however I have significant reservations, as outlined above.</p>
        </body>
    </sub-article>
    <sub-article article-type="reviewer-report" id="report485440">
        <front-stub>
            <article-id pub-id-type="doi">10.5256/f1000research.198722.r485440</article-id>
            <title-group>
                <article-title>Reviewer response for version 1</article-title>
            </title-group>
            <contrib-group>
                <contrib contrib-type="author">
                    <name>
                        <surname>D</surname>
                        <given-names>Esther Lydia</given-names>
                    </name>
                    <xref ref-type="aff" rid="r485440a1">1</xref>
                    <role>Referee</role>
                    <uri content-type="orcid">https://orcid.org/0000-0003-4467-2821</uri>
                </contrib>
                <aff id="r485440a1">
                    <label>1</label>Loyola College, Chennai, Tamil Nadu, India</aff>
            </contrib-group>
            <author-notes>
                <fn fn-type="conflict">
                    <p>
                        <bold>Competing interests: </bold>No competing interests were disclosed.</p>
                </fn>
            </author-notes>
            <pub-date pub-type="epub">
                <day>4</day>
                <month>6</month>
                <year>2026</year>
            </pub-date>
            <permissions>
                <copyright-statement>Copyright: &#x00a9; 2026 D EL</copyright-statement>
                <copyright-year>2026</copyright-year>
                <license xlink:href="https://creativecommons.org/licenses/by/4.0/">
                    <license-p>This is an open access peer review report distributed under the terms of the Creative Commons Attribution Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.</license-p>
                </license>
            </permissions>
            <related-article ext-link-type="doi" id="relatedArticleReport485440" related-article-type="peer-reviewed-article" xlink:href="10.12688/f1000research.180136.1"/>
            <custom-meta-group>
                <custom-meta>
                    <meta-name>recommendation</meta-name>
                    <meta-value>approve-with-reservations</meta-value>
                </custom-meta>
            </custom-meta-group>
        </front-stub>
        <body>
            <p>The study on impact of nutritional and organoleptic use of underutilized GLV in bakery products is well written and the paper follows a sound systemic review framework. However, I am suggesting minor revisions to strengthen the quality of the paper.&#x00a0;</p>
            <p> 1. The manuscript lacks a clear operational definition of UGLV. The authors should establish explicit criteria for classify UGLV and provide the list with their English names and scientific names.</p>
            <p> 2. The methodology lacks search strings and dates/years used for the screening procedure.&#x00a0;</p>
            <p> 3. The paper only presented qualitative synthesis of findings. Quantitative summaries of data on fiber, minerals, antioxdant values, sensory scores need to be incorporated. This will allow the readers to have better understand the magnitude of benefits of UGLV.</p>
            <p> 4. The manuscript states 8419 records were identified, 752 duplicates and 877 articles underwent screening. These number appears inconsistently mathematically. Need clarification on records removed for other reason.&#x00a0;</p>
            <p> 5. The discussion need to elaborate on the industrial applications as limited data has been provided on scalability, product development, shelf-life, FSSAI regulations involved and market value. Discussion commercialization pros and cons would give this paper more value.</p>
            <p>Are the rationale for, and objectives of, the Systematic Review clearly stated?</p>
            <p>Yes</p>
            <p>Is the statistical analysis and its interpretation appropriate?</p>
            <p>Not applicable</p>
            <p>If this is a Living Systematic Review, is the &#x2018;living&#x2019; method appropriate and is the search schedule clearly defined and justified? (&#x2018;Living Systematic Review&#x2019; or a variation of this term should be included in the title.)</p>
            <p>Not applicable</p>
            <p>Are sufficient details of the methods and analysis provided to allow replication by others?</p>
            <p>Yes</p>
            <p>Are the conclusions drawn adequately supported by the results presented in the review?</p>
            <p>Yes</p>
            <p>Reviewer Expertise:</p>
            <p>Functional Foods, Food Processing, Nutrition</p>
            <p>I confirm that I have read this submission and believe that I have an appropriate level of expertise to confirm that it is of an acceptable scientific standard, however I have significant reservations, as outlined above.</p>
        </body>
    </sub-article>
    <sub-article article-type="reviewer-report" id="report485444">
        <front-stub>
            <article-id pub-id-type="doi">10.5256/f1000research.198722.r485444</article-id>
            <title-group>
                <article-title>Reviewer response for version 1</article-title>
            </title-group>
            <contrib-group>
                <contrib contrib-type="author">
                    <name>
                        <surname>S</surname>
                        <given-names>Angeline Esther Preethi</given-names>
                    </name>
                    <xref ref-type="aff" rid="r485444a1">1</xref>
                    <role>Referee</role>
                </contrib>
                <aff id="r485444a1">
                    <label>1</label>RVS College of Arts and Science, Coimbatore, Tamil Nadu, India</aff>
            </contrib-group>
            <author-notes>
                <fn fn-type="conflict">
                    <p>
                        <bold>Competing interests: </bold>No competing interests were disclosed.</p>
                </fn>
            </author-notes>
            <pub-date pub-type="epub">
                <day>3</day>
                <month>6</month>
                <year>2026</year>
            </pub-date>
            <permissions>
                <copyright-statement>Copyright: &#x00a9; 2026 S AEP</copyright-statement>
                <copyright-year>2026</copyright-year>
                <license xlink:href="https://creativecommons.org/licenses/by/4.0/">
                    <license-p>This is an open access peer review report distributed under the terms of the Creative Commons Attribution Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.</license-p>
                </license>
            </permissions>
            <related-article ext-link-type="doi" id="relatedArticleReport485444" related-article-type="peer-reviewed-article" xlink:href="10.12688/f1000research.180136.1"/>
            <custom-meta-group>
                <custom-meta>
                    <meta-name>recommendation</meta-name>
                    <meta-value>approve</meta-value>
                </custom-meta>
            </custom-meta-group>
        </front-stub>
        <body>
            <p>
                <bold>Summary&#x00a0;</bold>
            </p>
            <p> This review article explores the potential of incorporating underutilized green leafy vegetables (UGLV) in bakery products for application as&#x00a0; functional foods.</p>
            <p> 1. The selection of articles for this manuscript is done in a systematic manner using scientific databases, Pub Med, Scopus, Google Scholar and web of Science.</p>
            <p> 2.The significance of adding the underutilized vegetables in improving the nutrient content, bio active compounds and functional ingredients is clearly described.</p>
            <p> 3.The challenges incurred on addition of these underutilized food ingredients on the functional and sensory attributes of bakery products is also clearly documented</p>
            <p> 4. The paper envisages to use food processing technologies to develop UGLV incorporated bakery products with better quality parameters&#x00a0;</p>
            <p> The manuscript is well written with systematic review and analysis and suitable for indexing.</p>
            <p>Are the rationale for, and objectives of, the Systematic Review clearly stated?</p>
            <p>Yes</p>
            <p>Is the statistical analysis and its interpretation appropriate?</p>
            <p>Yes</p>
            <p>If this is a Living Systematic Review, is the &#x2018;living&#x2019; method appropriate and is the search schedule clearly defined and justified? (&#x2018;Living Systematic Review&#x2019; or a variation of this term should be included in the title.)</p>
            <p>Not applicable</p>
            <p>Are sufficient details of the methods and analysis provided to allow replication by others?</p>
            <p>Yes</p>
            <p>Are the conclusions drawn adequately supported by the results presented in the review?</p>
            <p>Yes</p>
            <p>Reviewer Expertise:</p>
            <p>Food processing, Community nutrition, New product development</p>
            <p>I confirm that I have read this submission and believe that I have an appropriate level of expertise to confirm that it is of an acceptable scientific standard.</p>
        </body>
    </sub-article>
</article>
