Capsaicin from chili ( Capsicum spp.) inhibits vascular smooth muscle cell proliferation

Accelerated vascular smooth muscle cell (VSMC) proliferation is implied in cardiovascular disease and significantly contributes to vessel lumen reduction following surgical interventions such as percutaneous transluminal coronary angioplasty or bypass surgery. Therefore, identification and characterization of compounds and mechanisms able to counteract VSMC proliferation is of potential therapeutic relevance. This work reveals the anti-proliferative effect of the natural product capsaicin from Capsicum spp. by quantification of metabolic activity and DNA synthesis in activated VSMC. The observed in vitro activity profile of capsaicin warrants further research on its mechanism of action and potential for therapeutic application.


Main text
Aberrant and accelerated VSMC proliferation is a main contributor to restenosis, the pathological re-narrowing of the vessel lumen after surgical interventions combating vascular stenosis. To overcome restenosis, drug-eluting stents have been developed, aiming at inhibiting VSMC growth by the release of anti-proliferative substances such as paclitaxel and rapamycin. However, these compounds display unresolved issues such as impaired re-endothelialization and subsequent thrombosis induction 1 , which makes the characterization of other compounds able to suppress VSMC proliferation highly relevant. Plant-derived natural products are an excellent resource for identifying lead compounds 2 . Here we examine the anti-proliferative potential of capsaicin, a bioactive component of chili peppers [Capsicum spp. (Solanaceae)], in VSMC.
To test whether capsaicin is able to inhibit proliferation of VSMC induced by PDGF, a major growth factor implied in the aberrant proliferative responses in restenosis 3 , the total amount of metabolically active cells was measured after 48 h of incubation by the resazurin conversion method 4 . Capsaicin indeed suppressed VSMC proliferation concentration-dependently with an IC 50 of 5.36 μM ( Figure 1A). To confirm the anti-proliferative effect of capsaicin with a second experimental method, we measured DNA synthesis in VSMC by quantification of 5-bromo-2′-deoxyuridine (BrdU) incorporation into DNA. Capsaicin also inhibited PDGF-stimulated DNA synthesis in a concentration-dependent manner with an IC 50 of 3.81 μM ( Figure 1B). To assure that the decreased number of VSMC upon treatment with capsaicin is not due to cytotoxicity, we quantified cell death by measuring cell membrane integrity estimated by lactate dehydrogenase (LDH) activity inside cells and in cell supernatants. No significant cytotoxicity was detected in the investigated concentration range ( Figure 1C). In summary, capsaicin is identified as an inhibitor of VSMC proliferation. Further studies are prompted to elaborate the underlying mode of action of this natural product and to investigate its effect in advanced in vivo anti-restenotic models.
Rat aortic VSMC used in this study were purchased from Lonza (Braine-L'Alleud, Belgium) and cultivated in DMEM-F12 (1:1) medium supplemented with 20% fetal calf serum and gentamycin. Capsaicin and other chemicals were obtained from Sigma-Aldrich (Vienna, Austria).
For the resazurin conversion assay, VSMC were seeded in 96-well plates at 5 × 10 3 cells/well. 24 h later, cells were serum-starved for 24 h to render them quiescent. Quiescent cells were pretreated for 30 min with capsaicin or vehicle (0.1% DMSO) as indicated, and subsequently stimulated for 48 h with PDGF-BB (20 ng/mL). To measure the number of metabolically active VSMC by resazurin conversion 4 , cells were washed with PBS and incubated in serumfree medium containing 10 μg/mL resazurin for 2 h. Total metabolic activity was measured by monitoring the increase in fluorescence at a wavelength of 590 nm using an excitation wavelength of 535 nm in a 96-well plate reader (Tecan GENios Pro).  For assessing cytotoxicity, VSMC were seeded and serum-starved as indicated above. The quiescent cells were pretreated for 30 min with capsaicin, or vehicle as indicated, and subsequently stimulated for 24 h with PDGF-BB (20 ng/mL). To quantify the loss of cell membrane integrity as a sign for cell death 6 , the supernatants of the treated cells were assessed for LDH activity. For estimation of the total LDH, identically treated samples were incubated for 45 min in the presence of 1% Triton X-100.

Grant information
This work was supported by the EU-FP7 Marie Curie Fellowship 252881, and by the University of Vienna "Back-to-Research Grant" (both to R. Liu); as well as by the Austrian Science Fund (FWF): S10704, P25971-B23, and P23317-B11.
I confirm that the funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.