Method-centered digital communities on protocols.io for fast-paced scientific innovation

The Internet has enabled online social interaction for scientists beyond physical meetings and conferences. Yet despite these innovations in communication, dissemination of methods is often relegated to just academic publishing. Further, these methods remain static, with subsequent advances published elsewhere and unlinked. For communities undergoing fast-paced innovation, researchers need new capabilities to share, obtain feedback, and publish methods at the forefront of scientific development. For example, a renaissance in virology is now underway given the new metagenomic methods to sequence viral DNA directly from an environment. Metagenomics makes it possible to “see” natural viral communities that could not be previously studied through culturing methods. Yet, the knowledge of specialized techniques for the production and analysis of viral metagenomes remains in a subset of labs. This problem is common to any community using and developing emerging technologies and techniques. We developed new capabilities to create virtual communities in protocols.io, an open access platform, for disseminating protocols and knowledge at the forefront of scientific development. To demonstrate these capabilities, we present a virology community forum called VERVENet. These new features allow virology researchers to share protocols and their annotations and optimizations, connect with the broader virtual community to share knowledge, job postings, conference announcements through a common online forum, and discover the current literature through personalized recommendations to promote discussion of cutting edge research. Virtual communities in protocols.io enhance a researcher’s ability to: discuss and share protocols, connect with fellow community members, and learn about new and innovative research in the field. The web-based software for developing virtual communities is free to use on protocols.io. Data are available through public APIs at protocols.io.


Introduction
The Internet has enabled online social interaction for scientists beyond physical meetings and conferences. Twitter, Facebook, and ResearchGate 1-3 provide valuable online forums that many researchers use to share knowledge. At the same time, academic publishing remains time consuming and inefficient for communicating methodology. Protocols are often relegated to supplementary information, if shared at all. There is no good mechanism for easily discussing, troubleshooting, and improving published or unpublished techniques.
This need is even more apparent in emerging fields such as viral ecology where laboratory, field, and bioinformatics methods are being actively developed 4 . For example, new metagenomic techniques to sequence viral DNA directly from environmental samples has led to rapid advances in both molecular and bioinformatic protocols 5 . These protocols, however, are highly specialized and generally used in a few highly proficient labs because: (i) viral metagenomes (viromes) are difficult to produce due to low quantities of DNA and refined isolation and purification methods, (ii) the vast majority of viral sequences are unknown (usually >90% 6 ) complicating bioinformatics analyses, and (iii) newly emerging comparative and functional metagenomic analyses exist but require on-going community refinement and development.
Given the experimental nature of methods, the virology community has expressed a need to foster discussions about these protocols towards improved methodologies and increasing connectivity and collaboration among researchers 7 . The challenge is to develop a method-centered collaborative platform that recapitulates the functionality of a scientific meeting -a digital community for connecting with fellow researchers to share and discover state-of-the-art knowledge.
Here we describe new capabilities in protocols.io (http://www. protocols.io), an open access platform, to create virtual communities to disseminate protocols and knowledge at the forefront of scientific development. To demonstrate these capabilities, we describe a viral ecology community forum called VERVENet (https://www.protocols.io/groups/verve-net) that strives to increase connectivity and knowledge dissemination in viral ecology research at all levels from undergraduates to accomplished viral ecologists. These new community features enhance a researcher's ability to discuss and share protocols, connect with fellow community members, and learn about new and innovative research in the field. The web-based software for developing virtual communities is free for use on protocols.io, and further described here.

Protocols.io: a platform to enable methods discussion and dissemination
Protocols.io is a free service for industry and academic scientists to share or maintain private protocols for research 8 . The driving force behind software development is to provide a mechanism for scientists to share improvements and corrections to protocols, so that others are not continuously re-discovering knowledge that scientists have not had the time or wear-with-all to publish. Protocols.io provides a free, up-to-date, crowd-sourced protocol repository called protocols.io (http://www.protocols.io) for the life science community. This software is available as a web-based platform or smart phone App 9,10 to enable mobile solutions for research and bench-work. Per best practices in mobile computing, these Apps offer extensive options and control of push notifications. In fall 2014, protocols.io offered a well-developed platform for users to share molecular methods, however no capabilities were in place to share methods among groups, or bioinformatics methods. To this end, the viral ecology community teamed up with protocols.io to create new group capabilities, develop bioinformatics protocols, and enhance discussion forums for news, methods, and literature.

Introducing VERVENet: the Viral Ecology Research and Virtual
Exchange Network: The Viral Ecology Research and Virtual Exchange Network (VERVENet), is a collaboration between the University of Arizona and protocols.io, to deliver an online forum for the virology community. To enable this forum, new group functionality was built into protocols.io to promote scientific communication and collaboration. Specifically, group features were developed on top of existing capabilities to share molecular methods in order to (i) share protocols and their annotations and optimizations, (ii) fuel connectivity among viral ecology researchers for sharing data sets, knowledge, job postings, conference announcements through a common online forum called VERVENet and (iii) facilitate literature discovery through personalized recommendations to promote discussion on cutting edge viral ecology research. Through developing these interconnected resources in protocols.io for virtual communities, we developed a "go-to" site for viral ecology research 11 . Moreover, these tools are broadly useful to any community or individual lab for promoting scientific inquiry, reproduction of results, dissemination of protocols and re-use. Specifically, new forums can be created in a matter of minutes to enable connectivity among groups of any size, with tools described here under use cases. The VERVE Net forum is a place to discuss newly emerging methods in viral ecology for any kind of data such as: 'omics or image datasets. Yet, while images, videos and tables can be added to protocols/steps to enhance the description of methods, the protocols.io platform is not a data storage site.

Amendments from Version 1
The revisions made in this Version 2 are outlined below: 1. A statement at the end of the Introduction was added to describe the range of data intended for the VERVENet forum. 2. A paragraph was added in Methods called "Finding Protocols", to describe how users can use tags and search capabilities to find protocols of interest. 3. A paragraph was added in Methods called "Providing feedback on protocols", to describe three ways users can provide feedback on the forum including: social media, email, and a feedback forum on the protocols.io site. 4. Several words were removed from the Abstract and Introduction to rephrase/omit empathic language that social interactions for scientists only occur in scientific meetings per comments from Drs. Dunigan and Agarkova.

Methods
Creating a user profile in protocols.io: Users can view protocols and all public content anonymously, but to interact with the platform, registration is necessary. Registration is quick as only e-mail and password are required to create an account; however, users are encouraged to create profiles containing their name, website, affiliation, and research interests. Others can search and find a user based on name or keywords. Moreover, user profiles are attached to any material on protocols.io that the user posts publically. User profiles also contain a field for ORCID 12 , so that researchers can tie their profile back to a common identifier and highlight their work in the field. Researchers can also include a biography that describes how they got into the field and what intrigues them. Thus, profiles allow users to add in their own content, rather than simply browse existing content.
Adding protocols in protocols.io: After registration, new protocols can be entered ( Figure 1). By default, all protocols are private and can be shared with individual collaborators or any of the groups. The protocols are structured with tabs for the "steps", "description", 'guidelines', and "comments". When entering the steps, a list of components that can be added to the steps is located on the far right and allows a clear detailing of wetlab or computational portions of the method. Related steps of the protocol can also be easily grouped together into "sections" such as 'preparation,' 'DNA extraction,' and 'analysis', etc.
Steps may be entered one by one by typing into the text box or by pasting steps from another file, facilitating import of existing protocols. For each step annotations can be added to make notes on specific steps. Once complete, the protocol can be "run" in a step-by-step format.
Once a protocol has been created, there are several options for sharing it with collaborators or a group. To make the protocol publicly viewable, one will need to click the 'publish' button. A protocol can be reassigned to another individual with a protocols.io account.
For ongoing development and changes to adding and using protocols, see tutorials (http://www.protocols.io/help) in protocols.io 13 .
Developing groups in protocols.io: To create a group, one must have an account and be logged in. For example, here we describe the VERVE Net group, however it is possible to create any group.
To create a group, users can click on their personal icon in the upper right hand corner and select "+ new group." They will be prompted to enter a group name, image, description of the group, research interests, external website address, physical location of the group and an affiliation. The user will also decide if the group is open to anyone, by invitation only, or open to membership requests. In addition, the user can choose if the group is visible to others or private. Users are able to invite members into their group and control the privileges of their members. Moreover, as the owner of a group, the user is able to invite other subgroups, such as in the VERVE example individual labs are subgroups. Finding protocols: Protocols on protocols.io can be "tagged" to allow users to quickly find protocols or collections of protocols in a particular area of interest. Users can also find protocols or other content using the global search at the top of each page that allows users to search within the entire forum, or specific sections of the forum.
Providing feedback on protocols: protocols.io offers three methods for feedback directly from users: twitter, email to protocols developers (info@protocols.io), and through a feedback forum where users and developers alike can respond. These comments are then used to fuel future development. Further, protocols.io recently initiated an ambassadors program where power users (usually graduate students or postdocs) that are directly connected to diverse communities provide feedback from a user-perspective. Thus, future development is guided by community input from these sources.

Use case: VERVE Net: Virus Ecology Research and Virtual Exchange Network
Molecular and bioinformatics protocols: Often, detailed "tricks of the trade" associated with lab, field, and bioinformatics protocols are not well-described in publications, and at best are stashed in supplemental materials. Practical information associated with running these protocols under varied conditions cannot be curated, documented, or discussed among students, postdocs, technicians, and faculty working in virology. Moreover, knowledge on when to use a particular version of a given protocol is not easily captured.
Protocols.io provides a flexible mechanism wherein protocols can be documented in a stepwise fashion to easily pivot between molecular and bioinformatics methodologies, link to useful websites or code in Github 14 , or reference manuals or original source materials for protocols, as exemplified in the VERVENet forum.
The user entering the protocol may not necessarily be the author of the original method. However, by providing links to the primary work, users can attribute credit to the original author while at the same time adding their own updates to the method either while they enter it, or at a later time. Further, other users have the capability to add notes and warnings to existing protocols in protocols.io. This functionality includes a mechanism to email the protocol author for protocol troubleshooting. Corrections and updates made by the protocol authors and users automatically trigger notifications e-mailed to researchers who use that protocol. Lastly, users can "fork" or copy existing protocols for further refinement or alternate uses while still maintaining links back to the original for credit and reference. As such, the protocol is a living document for the community to reuse and continually refine.
For publication, authors have the option to enter detailed methods into protocols.io, issue a digital object identifier (DOI 15 ), and link to the protocols.io record from the Methods section. This practice is now being encouraged in journal submissions and by funding agencies.
Protocol collections: Because protocols are often used in conjunction with other protocols, protocols.io has the capability to link protocols into user-defined workflows. This is particularly important for publications that may use a collection of varied protocols (field, lab, and bioinformatics) that are derived from many sources (protocols from the user or other users). In providing a collection of protocols associated with a publication, the authors enable their work to be replicated, easy to follow, and transparent to other members of the community in a way that can be referenced and cited. For example, a collection of protocols derived from a recent publication on the human skin double stranded DNA skin virome is available in VERVENet 16,17 . Thus, collections provide a mechanism for furthering open-science efforts.
Protocol collections also provide a mechanism to "learn by example" for early career scientists or those branching into a new area of scientific inquiry. In particular, detailed protocols associated with a toolkit or workshop, where multi-media options such as slides, video, or links to virtual machines with example datasets and code can be included 18,19 . This is particularly important for bioinformatics protocols that often include multiple programs and steps in an analysis for a given publication. Further, individual tools may have a collection of protocols that describe specific use-cases, example datasets, and varied options that they may wish to convey to their users.
Groups and sharing: Individual members can form groups, where the owner has the ability to choose the level of accessibility for fellow members. The groups can share literature recommendations, discussions, protocols, news, events, and job opportunities ( Figure 2). Subgroups can form under the umbrella of a larger group with a common interest. This subgroup/supergroup relationship allows smaller group activities to be shared with a larger virtual community with common interests. In the case of VERVENet, this supergroup links the broader research in virology with the subgroups of individual labs and more specific research interests such as plant viruses.
Literature recommendations: Each of the groups includes a literature recommendation system. This algorithm provides personalized publication recommendations based on a library from a user or group. This algorithm is used to develop "libraries" for viral ecology user groups, that will continually recommend new publications based on growing reading lists from individual users that are part of the group. This functionality allows virologists to make their reading lists public therefore helping new scientists joining the field in their topic area. The libraries from "sub-groups" also fuel the shared public reading list within the VERVENet group, therefore creating enhanced fluidity and cross-posted content between the groups.
Live online discussion forum: Each of the groups in protocols.io contains a live on-line discussion. Discussions can be generated directly on the discussion tab, or are cross-posted from discussions on specific protocols, news, or literature. Each of the discussions can reference outside websites, manuals, or online resources. This discussion forum enables users to discuss tips and tricks for specific protocols, review reagents linked to particular protocols, and reference outside resources that were not included in the original protocol.
Protocols.io also includes "journal-club" capabilities to enable on-line discussions of published research by researchers and authors. Other unique features in protocols.io include: career advice forum including a panel of mentors 20 and a "behind the article" essay forum 21 . These communication forums allow researchers to share their stories about how papers, protocols, or research efforts came about, that are both interesting to the community and informative for early career scientists.
Platform infrastructure and interoperability: Computers, tablets, and smart phones are becoming fundamental tools for scientists today. Furthermore, social networking and shared cyberinfrastructures are offering powerful new mechanisms to connect communities and science from across the world. Protocols.io leverages these powerful new tools and software capabilities to provide an online forum for viral ecology research to connect and share knowledge and resources. All components of protocols.io and the VERVENet forum are mobile-friendly and interoperable for use on diverse devices in the lab, on the desktop, or on the go. International Society for Viruses of Microorganisms has listed VERVE Net on their website 24 as a resource.

Discussion and conclusions
The primary goal of new group functionality in protocols.io is to provide a robust web-application for sharing up to date protocols, literature, and community features (news, jobs, discussions). This work is exemplified in VERVE Net, a virtual community forum for virology. Fundamental to this goal is the ability for researchers to establish groups based on similar interests and share knowledge, without apriori knowledge of key members in a given field.
We have designed an infrastructure that has multiple entry points for establishing relationships among users ranging from selfproclaimed groups or areas of interest, to options to join groups maintained by others in an area of interest to the user fueled by related protocols or reading lists. Moreover, news feeds about funding opportunities, job postings, or collaborative research opportunities can be fine-tuned according to interest. These connections will allow the forum to evolve naturally given rapidly developing trends and new protocols. Protocols.io is open access and is both, free to read and free to publish. The revenue and sustainability model is based on the sale of data services to reagent vendors (most popular protocols, protocol improvements, and reagent-protocol links). Protocols.io also charges fees for private non-academic groups.
Protocols.io is a central resource to connect, collaborate, share and innovate within virtual communities. The VERVENet forum demonstrates how this new group functionality allows researchers to promote scientific inquiry, reproduction of results, and dissemination and optimization of both molecular and bioinformatics protocols, as a virtual community.

Data and software availability
Protocols.io and the VERVENet commuity forum are committed to open access for data content and interoperability. To that end, the content in protocols is available through an Application Programming Interface (API) for advanced data mining and no registration is required to view protocols, comments or annotations. All public protocols are archived with CLOCKSS for long-term digital preservation 25 . Users will also be able to access public protocols.io mirrored at the Center for Open Science.

Author contributions
LK wrote the manuscript, tested the platform, added content and provided feedback on features and functionality. AS developed the platform, tested and designed features and functionality. LT and BLH designed VERVENet, tested the system, provided feedback on features and functionality, and wrote the manuscript. All authors read and approved the final manuscript.

Competing interests
Leonid Teytelman and Alexei Stoliartchouk are employees of protocols.io and both own equity in the company.

Grant information
This work was funded by a grant to B.L.H. and L.T. from the Gordon Betty Moore Foundation (GBMF4733).
The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. This well-written report describes the creation of a new platform for facilitating collaborative work among and between research groups. This platform takes advantage of the existing protocols.io site and App, and so is focused on methodological sharing, although other types of information are also covered. This approach fits well with the recent emphasis (from NIH and others) to address the issue of reproducibility in the biomedical literature. Of course, the ultimate utility of this (or any) platform will depend on what those in the field make it.

Open Peer Review
One item that the authors may wish to clarify is the protocols.io business model, which is touched upon near the end of this piece, but is likely not familiar to many readers. This is not meant to impugn the data services model, but just to ensure its transparency.
No competing interests were disclosed.

Competing Interests:
I have read this submission. I believe that I have an appropriate level of expertise to confirm that it is of an acceptable scientific standard.  There are some concerns that may need further clarification in this publication relating to 1) navigation, 2) types of data being supported, and 3) intellectual property rights.
At a practical level, VERVENet is still in its infancy and one would expect it to continue to develop in response to the community input. Thus, the concerns we have with navigating in VERVENet at this time will likely be corrected/ adjusted with continued use and input by users. The authors should address how they intend to respond to the intended community when suggestions are proposed. Who has the "authority" to make the changes and how will those changes be made available? Given the potential (and presumed intended) for a highly dynamic forum, will there be an archive of information maintained?
It is not quite clear from the manuscript how navigation by particular topics works. Is it possible to find a specific protocol without having to scroll through all entries, as well as to find easily a collection of protocols related to a particular field, e.g,. nucleic acid isolation and manipulation; proteomics; bioinformatics, etc? If these functions exist in the current version of VERVENet, it would help to describe this more fully.
The authors indicate that VERVENet was initiated, in part, due to "…a renaissance in virology..given the new metagenomic methods..." Although it is true that there is a large influx of metagenomic data in recent years, DNA sequence data is not the only type of data of interest to viral ecologists. The authors should discuss what types of data are being supported. For example, how are image data sets being handled, given the relatively large file size? How will VERVENet adapt as other types of 'omics' data become common within the viral ecology community? Clearly, DNA sequence is not the only data of interest, RNA sequence is equally important to virologists, so 1 2 1 2 4.
DNA sequence is not the only data of interest, RNA sequence is equally important to virologists, so the authors may wish to indicate the range of data intended for VERVENet.
The authors have taken on the responsibility of creating an online forum with an emphasis on methods. These methods are created by individuals and generally made available to anyone whether they register with protocols.io or not, at varying levels depending on the authors' decisions. It is not clear who holds the intellectual property rights at that point. The authors should be transparent about the rights of the protocols.io contributing authors, where the legal authority begins and ends, and how contributors can both share and protect their intellectual property should they choose. Another concern that should be addressed is what is the fate of VERVENet should protocols.io change ownership. Are there contingency plans in the company statutes that would ensure that groups like VERVENet be supported? What happens if they are not supported? Who has legal ownership the information? Also, given that the current funding is through the Gordon and Betty Moore Foundation, what happens to the content of VERVENet should that funding be removed or reduced? These things need some clarification.
The title of the paper does not give a full reflection of the contents of the paper.
There are several statements in the manuscript where the authors tend to use emphatic language that is not easily supporter. For example, the first sentence of the Abstract infers that social interactions of scientists ONLY occurs at physical meetings and conferences. This statement is not only inaccurate, it undermines the credibility of the information that follows. The authors should re-read their paper and consider how some of their statements may be perceived. There are several examples.
No competing interests were disclosed.

Competing Interests:
We have read this submission. We believe that we have an appropriate level of expertise to confirm that it is of an acceptable scientific standard, however we have significant reservations, as outlined above. Thank you for your time in reviewing our manuscript. Please see our comments to your suggested revisions below: : At a practical level, VERVENet is still in its infancy and one would expect it to Comment 1 continue to develop in response to the community input. Thus, the concerns we have with navigating in VERVENet at this time will likely be corrected/ adjusted with continued use and input by users. The authors should address how they intend to respond to the intended community when suggestions are proposed. Who has the "authority" to make the changes and how will those changes be made available? Given the potential (and presumed intended) for a highly dynamic forum, will there be an archive of information maintained?
: In terms of the software, protocols.io offers three methods for feedback directly from Response 1 users: twitter, email to protocols developers (info@protocols.io), and through a feedback forum where users and developers alike can respond. These comments are then used to fuel future development. Further, protocols.io recently initiated an ambassadors program where power users (usually graduate students or postdocs) that are directly connected to diverse communities provide (usually graduate students or postdocs) that are directly connected to diverse communities provide feedback from a user-perspective. VERVE Net is one such community and currently has an ambassador to further develop the community. Thus, future development is guided by community input from these sources. We have amended the manuscript to clarify this important point. Information from the forum is maintained through the CLOCKKS digital archiving project ( ) as described in the manuscript under "Data and software https://www.clockss.org/clockss/Home availability".
It is not quite clear from the manuscript how navigation by particular topics works. Is it Comment 2: possible to find a specific protocol without having to scroll through all entries, as well as to find easily a collection of protocols related to a particular field, e.g,. nucleic acid isolation and manipulation; proteomics; bioinformatics, etc? If these functions exist in the current version of VERVENet, it would help to describe this more fully.
Protocols on protocols.io can be "tagged" to allow users to quickly find protocols or Response 2: collections of protocols in a particular area of interest. Users can also find protocols or other content using the global search at the top of each page that allows users to search within the entire forum, or specific sections of the forum. We have updated the text to clarify the search features.
: The authors indicate that VERVENet was initiated, in part, due to "…a renaissance in Comment 3 virology..given the new metagenomic methods..." Although it is true that there is a large influx of metagenomic data in recent years, DNA sequence data is not the only type of data of interest to viral ecologists. The authors should discuss what types of data are being supported. For example, how are image data sets being handled, given the relatively large file size? How will VERVENet adapt as other types of 'omics' data become common within the viral ecology community? Clearly, DNA sequence is not the only data of interest, RNA sequence is equally important to virologists, so the authors may wish to indicate the range of data intended for VERVENet.
: Indeed, VERVENet is not just a forum to discuss 'omics datasets but is inclusive of Response 3 all types of data on studying viruses. The forum is meant to be a place to discuss newly emerging methods in viral ecology that includes but is not limited to 'omics datasets. Also, while images, videos and tables can be added to protocols/steps, the protocols.io platform is not a data storage site like dropbox, GitHub, figshare, datadryad or CyVerse. To clarify, we added several sentences in the introduction.
: The authors have taken on the responsibility of creating an online forum with an Comment 4 emphasis on methods. These methods are created by individuals and generally made available to anyone whether they register with protocols.io or not, at varying levels depending on the authors' decisions. It is not clear who holds the intellectual property rights at that point. The authors should be transparent about the rights of the protocols.io contributing authors, where the legal authority begins and ends, and how contributors can both share and protect their intellectual property should they choose. Another concern that should be addressed is what is the fate of VERVENet should protocols.io change ownership. Are there contingency plans in the company statutes that would ensure that groups like VERVENet be supported? What happens if they are not supported? Who has legal ownership the information? Also, given that the current funding is through the Gordon and Betty Moore Foundation, what happens to the content of VERVENet should that funding be removed or reduced? These things need some clarification.
: All public content on protocols.io is open access and is clearly labeled as CC-BY at Response 4 the footer of each page. Moreover, the Terms of Service (https://www.protocols.io/terms#tos1) are the footer of each page. Moreover, the Terms of Service (https://www.protocols.io/terms#tos1) are explicit: "We claim no intellectual property rights over the material you provide to the Sites/Apps. Your profile and materials uploaded remain yours. However, by publishing your protocols to be viewed publicly, you agree to allow others to view your Content. By setting your protocols to be viewed publicly, you agree to allow others to view and fork your protocols." Content generated by our users belongs to the users and is licensed under the Creative Commons Attribution License (CC-BY). As discussed above, the archiving via CLOCKSS and the mirroring with COS are meant to ensure long-term digital preservation of all knowledge on protocols.io, even in the case of bankruptcy or change of control.
VERVENet does not rely on continued funding from GBMF. The support for this and other groups comes from the revenue streams at protocols.io. Ensuring long-term sustainability, independent of grants from funding agencies was an important component of the original VERVE grant application.
: The title of the paper does not give a full reflection of the contents of the paper.

Comment 5
: The title reflects the general application of developing digital communities in Response 5 protocols.io for which VERVENet is an example. We kept the title more general to reflect the broad utility of the forum. While VERVENet was the first group on protocols.io and the grant for it enabled the creation of the functionality described here, it is meant for wide use and has already growth to more than 250 public and private groups and communities such as , , , PROT-G Open Plant MinION and others.
: There are several statements in the manuscript where the authors tend to use Comment 6 emphatic language that is not easily supporter. For example, the first sentence of the Abstract infers that social interactions of scientists ONLY occurs at physical meetings and conferences. This statement is not only inaccurate, it undermines the credibility of the information that follows. The authors should re-read their paper and consider how some of their statements may be perceived. There are several examples.
: Thank you for pointing this out. Social interactions at conferences was only meant to Response 6 be an example of the many interactions scientists have daily. We updated the manuscript to omit/rephrase any emphatic language per your suggestion.

No competing interests
Competing Interests: