Preliminary results on the control of Aedes spp. in a remote Guatemalan community vulnerable to dengue, chikungunya and Zika virus: community participation and use of low-cost ecological ovillantas for mosquito control

Objective: To study the effectiveness of an integrated intervention of health worker training, a low-cost ecological mosquito ovitrap, and community engagement on Aedes spp. mosquito control over 10 months in 2015 in an urban remote community in Guatemala at risk of dengue, chikungunya and Zika virus transmission. Methods: We implemented a three-component integrated intervention consisting of: web-based training of local health personnel in vector control, cluster-randomized assignment of an ecological modified ovitrap (ovillantas: ovi=egg, llanta=tire) or standard ovitraps to capture Aedes spp. mosquito eggs (no efforts have been taken to determine the exact Aedes species at this moment), and community engagement to promote participation of community members and health personnel in the understanding and maintenance of ovitraps for mosquito control. The intervention was implemented in local collaboration with Guatemala’s Ministry of Health’s Vector Control Programme, and in international collaboration with the National Institute of Public Health in Mexico. Findings: Eighty percent of the 25 local health personnel enrolled in the training programme received accreditation of their improved knowledge of vector control. When ovillantas were used in a cluster of ovitraps (several in proximity), significantly more eggs were trapped by ecological ovillantas than standard ovitraps over the 10 month (42 week) study period (t=5.2577; p<0.05). Repetitive filtering and recycling of the attractant solution (or water) kept the ovillanta clean, free from algae growth. Among both community members and health workers, the levels of knowledge, interest, and participation in community mosquito control and trapping increased. Recommendations for enhancing and sustaining community mosquito control were identified. Conclusion: Our three-component integrated intervention proved beneficial to this remote community at risk of mosquito-borne diseases such as dengue, chikungunya, and Zika. The combination of training of health workers, cluster use of low-cost ecological ovillanta to destroy the second generation of mosquitoes, and community engagement ensured the project met local needs and fostered collaboration and participation of the community, which can help improve sustainability. The ovillanta intervention and methodology may be modified to target other species such as Culex, should it be established that such mosquitoes carry Zika virus in addition to Aedes.

We implemented a three-component integrated intervention Methods: consisting of: web-based training of local health personnel in vector control, cluster-randomized assignment of an ecological modified ovitrap (ovillantas: ovi=egg, llanta=tire) or standard ovitraps to capture mosquito eggs Aedes spp. (no efforts have been taken to determine the exact species at this Aedes moment), and community engagement to promote participation of community members and health personnel in the understanding and maintenance of ovitraps for mosquito control. The intervention was implemented in local collaboration with Guatemala's Ministry of Health's Vector Control Programme, and in international collaboration with the National Institute of Public Health in Mexico.
Eighty percent of the 25 local health personnel enrolled in the Findings: training programme received accreditation of their improved knowledge of vector control. When ovillantas were used in a cluster of ovitraps (several in proximity), significantly more eggs were trapped by ecological ovillantas than standard ovitraps over the 10 month (42 week) study period (t=5.2577; p <0.05). Repetitive filtering and recycling of the attractant solution (or water) kept the ovillanta clean, free from algae growth. Among both community members and health workers, the levels of knowledge, interest, and participation in There is increasing concern about mosquito-borne disease, amplified by the recent Latin American outbreaks of Zika virus, which have raised new alarm about their rapid spread and illness in vulnerable populations 1 . The fact that globalization increases vector migration has been demonstrated with the first finding of mosquitoes infected with the African West Nile virus in 2009 in the New York City region 2 . The Americas have seen invasion of dengue virus, chikungunya virus, and most recently the Zika virus, causing dangerous outbreaks and subsequent morbidity and mortality that have proved difficult to control in a sustainable manner.
All of these viruses, plus the yellow fever virus, are transmitted mainly by mosquitoes of the Aedes genus (subgender: Stegomyia), more specifically the African species Aedes aegypti, one of the most aggressive vector mosquitoes capable of transmitting these illnesses, and to a lesser level the Asian tiger mosquito, Aedes albopictus, which prefers to bite during the day and is emerging as one of the most adaptable insects in the world 3-5 . The rapid rise of virus transmission on the American continent is due partly to the lack of historical immunological experience (have not been exposed to the viral infection) or known cross-immunization to the different species and viral genotypes 6,7 of the people in the Americas. A second factor affecting the transmission is the stationary abundance of the vector Ae aegypti and other species susceptible to viral infection (e.g. Culex spp?). Furthermore, the traditional control method -pesticide -is both indiscriminate and thwarted by the multiple cases of pesticide resistance in Aedes mosquitoes recently reported 8,9 .
It is believed that Ae aegypti mosquitoes arrived to America with the Europeans during the colonization on the 16 th century 10a . It was Cuban Dr. Carlos Juan Finlay, working with Dr. Walter Reed, who identified the Ae. Aegypti as the culprit of the yellow fever outbreaks in Haiti and Panama in the 19 th century 10b , The rapid spread of several viri to most of the American continent began around the year 2000 10c . Now a days dengue fever is active in more than 128 countries 11 , chikungunya virus in 40 countries 12 , and the Zika virus in currently 26 countries and rising 13 . So far, we have not been able to stop it's advance or vector potential. What solutions are available?
Several methods have been employed for detecting the presence of mosquitoes in the field, but none have proved perfect. Standard ovitraps to monitor Aedes mosquitoes are large 1-litre black buckets ( Figure 1) filled with plain water or attractant solutions based on natural plant infusions, along with a wooden strip or porous pellon paper, which the mosquito lands on and lays its eggs (called oviposition) 14,15 .
Human landing techniques have also been used, but less so now due to ethical concerns. The BG-Sentinel trap is a newer mosquito monitoring device, which has proved similar or better to the humanlanding method for the detection of mosquitoes such as Anopheles darlingi 16 .
Of these monitoring methods, only the standard ovitraps have thus far proved to effectively reduce the amount of adult mosquitoes when they are used for extended periods and the ovitraps are continually surveyed and maintained, a very expensive and timeconsuming exercise 17 .
To overcome these limitations, other ovitraps have appeared on the market -some force the larvae to a confined, flooded partition where they die because they cannot reach the surface and breathe. Others contain glue or larvicidal compounds, chemical or biological (e.g. Bacillus thuringensis, fish 'Poecillia maylandi'), to rapidly destroy the larvae. Each of these new traps has monitoring merits, Typically, the water or attractant solution used in these ovitraps is removed and expelled into the ground each time the ovitrap is surveyed, and as a result any larvae or pupae are destroyed on the dry soil. The collection of the eggs "glued" to the landing strip allows for counting and thus monitoring. Two problems have been identified with this "dumping" of the solution. First, the need for clean replacement water is often problematic in remote areas without the basic infrastructure, and second, any eggs dislodged from the landing strip onto the dumped water or solution represent the possibility of continuous reproduction of mosquitoes. It is known that Aedes eggs can survive on the dry ground for several months 19 , thus creating a compromised situation of perpetual reproduction.
To address these problems, we determined that filtration and recycling of the solution (either water or attractant) seemed to be an optimal alternative, and we set about developing a modified ovitrap. The most attracting solution to Aedes mosquitoes is the solution that has had larvae of the same species (conspecific larvae), making the recycling of the solution more attractive to female mosquitoes 20 .
The development of our modified ovitrap at Laurentian University in Sudbury, Canada was initially geared to the reduction of the local West Nile virus vector, mainly the species Culex pipiens and Culex restuans. A 90% selective reduction of the adult mosquitoes of these species was achieved over three months around the site where the ovitraps were installed in 2008 (unpublished report, Ulibarri et al.).
The attractant solution used in these modified ovitraps was based on the fermentation of natural plants spiked with homemade chemicals known to be oviposition-attractive to these species, which we also developed. The modified ovitrap is commercially available in Canada (Green Strike; http://green-strike.com/products/mosquitopreventer-2) together with the solutions that selectively attract the local Culex spp. (Cu-Lure™) or Aedes spp. mosquitoes (Ae-Lure™) (e.g Aedes vexans/Ochlerotatus canadiensis). The attractant has the advantage of being both non-toxic and environmentally sensitive.
The same principles driving the Canadian ovitrap were later used in a field study during 2013-2014 in Petatlán, Mexico against the dengue fever mosquitoes Ae albopictus and Ae aegypti. The Aedes oviposition was reduced by 71% on the site with the modified ovitraps, compared to oviposition in a control site (unpublished report, Ulibarri et al.).
Building on these previous studies, we undertook a field study in Sayaxche, Guatemala beginning in February 2015. We were determined to use an integrated approach that involved education of the community and health workers responsible for vector control about the mosquito cycle, sustainable strategies for keeping homes and gardens clean and unattractive to mosquitoes, the implementation and maintenance of mosquito traps, and collaboration between community and health sectors to collectively manage vector control and prevention of mosquito borne disease. Here we describe our project and its results.
When we embarked on the collaborative project, two challenges arose immediately. First, we discovered that local health workers required more extensive training in vector control than initially thought. This was promptly solved when the Instituto Nacional de Salud Publica (INSP) in Cuernavaca, Mexico offered to share a beta version of a web-based programme they created with the International Development Research Centre (IDRC) in Canada, to provide vector control and health information in Spanish (see Annex 1).
Second, we were unable to procure our original ovitraps from Canada, and we needed to search for an appropriate alternative. We decided to use recycled tires -partly because tires represent up to 29% of the breeding sites chosen by Ae aegypti mosquitoes, and it also gave us the opportunity to recycle some old tires that were littering the local environment 21,22 . Modifying the ovitrap with tires gave birth to the ovillanta, a piece of a tire fitted with a valve to help direct the attractant solution to a filter ( Figure 1).

Methods
For this project, we designed the intervention to include three components in an integrated fashion: training health workers in vector control, low-cost ecological mosquito ovillanta, and community engagement. We sought to determine the effectiveness of this integrated intervention on Aedes spp. mosquito control over 10 months in 2015 in an urban remote community in Guatemala at risk of dengue, chikungunya and Zika virus transmission.
Our project was enabled by intersectoral collaboration between academic researchers, local health authorities from the Ministry of Health Vector Control Programme of Guatemala. international collaborators in Canada, Guatemala and Mexico, and community members. Our broad goal was to empower the community, supported by trained health personnel, to adopt the administration, care and maintenance of the health of the community.
Ethics. This study was reviewed and authorized by the representatives of the Program on Vector-Borne Transmitted Diseases of the Ministry of Public Health and Social Assistance of Guatemala (Programa de Enfermedades Trasmitidas por Vectores del Ministerio de Salud Publica y Asistencia Social (ETV/MSPAS) de Guatemala). The MSPSA follows guidelines adopted from the Reglamento del Sub-Comité de ética e investigación (www.paho.com) 23 . We interviewed and invited each of the health workers to participate in the web-based training and encouraged them to voluntarily subscribe online to the program. We personally engaged people within the community, always accompanied by workers from the Health Unit of Sayaxche. It is customary in the region to first engage the representatives of the community by a verbal invitation to sit down and discuss the project. Once permission was obtained, we randomized the houses, and invited adults to voluntarily participate, assuring them that if there was anything that caused discomfort we would withdraw them immediately from the program. All invitations, focus groups, and interviews were verbally carried out in Spanish and recorded on a portable recorder, and transcribed soon after. When necessary, an interpreter was present for interviews with non-Spanish speaking community members. We, accompanied by representatives of the community leaders, explained the benefits of their participation and their responsibilities, allowing them to voluntarily join the study project. The protocol for the community engagement/social participation evaluations was also presented and approved by the ETV/MSPAS representatives.

Study area.
Sayaxche is a remote urban center of the southwestern part and health unit area of the Peten territory in Guatemala. It borders the states of Chiapas and Tabasco in Mexico. Sayaxche's Q'eqchi Maya origin means the 'Ceiba's wye or fork.' The surface is 3752 km 2 and occupies 10.9% of Peten's territory. It is 250 m above sea level, with a warm, varied and humid tropical climate, with a rainy season (June to November) and a dry season (December to May). The monthly average temperature varies between 23°C (December/January) and 32°C (in the dry month of May).

Intervention.
Our three-component integrated intervention consisted of: a) web-based training of local health personnel in vector control, b) cluster-randomised assignment of ecological ovillantas or standard ovitraps to capture Ae aegypti mosquitoes, and c) community engagement to promote participation of community members and health personnel in the understanding and maintenance of ovitraps for mosquito control.
a) Web-based training of local health personnel in vector control (Annex 2). As we embarked on our project we consulted with the local health authorities, including the manager of the Vector Control Programme in Sayaxche, to determine the needs of the personnel. These included the need for improved technical skills, liaising with community including cultural sensitivity, and promoting and implementing programmes, as well as a strong need to improve the efficacy of the preventive measures of vector transmission in the region. As mentioned earlier, the health workers had higher educational needs than we had initially expected.
To meet these needs, we collaborated with academic leaders at the INSP and with colleagues in the EcoHealth Leadership Initiative on Vector Borne Diseases for Latin America and the Caribbean, which is part of IDRC. We developed learning objectives, a web-based platform and a bibliography of resources to help strengthen the technical skills of the health personnel in the vigilance, prevention and control of vector-borne diseases using an ecosystem approach that aims for sustainability and is tailored to local community needs (see Annex 1 and Annex 2 for more details of the curricular planning). The web-based training modelled a dynamic process of teaching-learning, incorporating both theoretical and practical aspects of how to prevent and control vector-borne diseases. It utilized diverse learning strategies with specific content sessions, directed homework, and literature review. It took place over 5 months (April to September) in 2015, covering 40 hours in total, with 50% of practice and homework on a virtual platform.
We measured improved knowledge and skills of local health personnel in vector control by their successful accreditation following the web-based training programme.
b) Control of vector mosquitoes using ovillantas. We developed an ecological ovillanta (a modified ovitrap: ovi=egg, llanta=tire in Spanish) from recycled tires, and compared this device to standard ovitraps in terms of mosquito oviposition. We quantified egg collection from standard ovitraps on sites with ovillantas and compared it to standard ovitraps on sites without ovillantas.
We modified the standard ovitrap to create ovillantas, made out of a piece of recycled tire with a PVC flanged wash basin draintype tube and a valve, for the capture of the vector mosquito's eggs (Ae aegypti). The attractant solution called AE-Lure (8mL) was placed on the ovillanta and two litres of clean well water were added. Each ovillanta contained two landing strips -one on each end of the apparatus. For the landing strips we used 15×10 cm pieces of pellon paper, but other porous material might be used.
We compared the ecological ovillantas with the standard ovitrap, which was a black bucket containing one litre of clean well water with one piece of the same pellon paper (but of a larger size, 30×10 cm) around the rim.
The urban core of Sayaxche contains 15 neighbourhoods, which comprised 14,454 inhabitants within 3,882 houses. We designed the random sampling based on our previous study in Petatlán, Mexico (unpublished report, Ulibarri et al.) and modified it to fit Sayaxche, applied as follows: 14 neighbourhoods (out of 15) were divided in half making two separate groups, the study group and the control group. Within each neighbourhood, 3 continuous blocks of houses were randomly chosen and one house at the center of each side of the block (north, east, south and west) was consulted to set up either 2 ovillantas and 1 standard ovitrap (study site) or 1 standard ovitrap per house (control site). If a chosen house declined to participate, the next house was invited. In the end each neighbourhood had 12 households participating. The total number of sites with ovillantas/ovitraps were 84 for the study group and 84 for the control group (see Table 1 for the names of the neighbourhoods).
Once the ovillantas and ovitraps were installed in the different households, the health workers started cleaning the ovillantas in the presence of household members and, later on, as interest increased, household members themselves were responsible for cleaning the ovillantas or ovitraps, in the presence of health workers. At the beginning of our project, the joint care and egg collection was carried out once a week, on a day pre-established with the community. During the high season (June-October), it was necessary to monitor and clean the ovillantas and ovitraps twice a week, in order to avoid the production of mosquitoes due to the rapid development of the larvae during high temperatures. The pellon papers containing the eggs were removed and given to the health workers, and new clean pieces of paper were installed. The papers with the eggs were taken to the laboratory of the local health unit by the health workers, and counted there by specialized personnel.
At the beginning of the project we started with three different types of mosquito traps: an ecological ovillanta with AE-Lure, an ecological ovillanta with Malaria-Lure, and the standard ovitrap with water. But shortly after initiation, we discovered Malaria-Lure to be ineffective for malaria-parasite carrying Anopheles mosquitoes. Therefore, by week 4 we changed all ecological ovillantas to include AE-Lure only.
We measured mosquito control by the mean number of eggs counted in ecological ovillantas versus standard ovitraps during 31 collections over 10 months between Feb and Nov 2015. Egg counting was undertaken once a week.
c) Community engagement. Through training, our intersectoral multidisciplinary approach aimed to improve and sustain the technical competencies of health workers. Through the modification of the ovitrap, we were sensitive to cost, ecological concerns, and local applicability. For both of these integrated interventions to work, community involvement was required. Therefore, we simultaneously undertook community engagement activities, recognising the importance of sustained social participation by both community members and health workers.
We developed strategies to engage the main representatives of the community, in order to motivate participation in the mosquito control programme. We focused on education of the community (in addition to health workers) about the mosquito cycle, sustainable strategies for keeping homes and gardens clean and unattractive to mosquitoes, the implementation and maintenance of mosquito traps, and collaboration between community and health sectors to collectively manage vector control and prevention of mosquitoborne disease. Specifically, community members were taught how to safely keep the ovillantas or ovitraps, including how to clean the traps, change the pellon paper and hand the pellon paper to the health workers. They were incentivised by the education they received on how to reduce the amount of mosquitoes and thus keep themselves and the community healthy. Another strong incentive was the absence of fogging, thus no pesticides in their neighbourhood, unless they did not care for the ovillantas properly and there were too many mosquitoes.
We measured social participation by surveying community member and health worker perceptions, knowledge and participation in mosquito control using qualitative methods. (see Annex 3 for fuller details of the evaluation of social participation). Focus groups were undertaken in eight neighbourhoods, four from the study site and four from the control site. Within each neighbourhood, one person from 24 randomly selected households (12 households with either ovillantas or ovitraps and 12 households that did not have either, but were located within the neighbourhoods of the study) was invited to participate in a focus group. Of the sixteen focus groups, the number of participants ranged from three to eight household members each.
In addition, qualitative interviews were conducted with three health workers who undertook the training and participated in the mosquito control programme, and one programme manager. Evaluation of social participation and analysis of the qualitative data was carried out at the beginning (February 2015) during the setup of the ovillantas and ovitraps in each household, in order to establish a base level for evaluation. This was followed by a second interview at the end of the project (December 2015). The interviews were carried out following the protocol validated by the ETV/MSPAS representative and were conducted by an experienced researcher independent from the other two components of the project (ovillanta study, web-based training) (MB).

Data analysis.
We analysed quantitative variables (egg count) and categorical variables (area, type of trap) for their frequency of distribution, measuring the central tendency with a graphical representation and distribution tests. The software package Stata13 ® was used to carry out the t-tests, which analyze the difference between two means and the dispersion of the scores (see Annex 4).
With respect to the qualitative data, we undertook an analysis per topic to understand perceptions, social participation, usefulness of strategies for control and prevention of the diseases, negative and positive factors of the control programme, and recommendations for future strategies.
Entomological poll. A survey of potential breeding sites was conducted in each house of the intervention neighbourhoods, where the ovillantas were placed. Each container capable of accumulating water (drums, tires, cans, etc.) was analysed and larvae/pupae were counted if present. A percentage of each type of container that was positive was established compared to the total number of containers found (see Annex 5).

a) Web-based training of health workers in vector control.
Twenty five health workers subscribed to the web-based training programme (of 35 eligible). Most had obtained elementary school level education (67%); the remainder had achieved junior high school level (18%), high school (10%), or university undergraduate level (5%). Following the web-based training (comprised of online lectures, case studies, virtual discussions and homework) teams of students worked on a proposed ecological intervention for the prevention and control of the vector mosquito in Sayaxche using a manual validated by the academic unit of the INSP (Annex 1) and were evaluated (directly by the INSP evaluation personnel, independently from the project's participants). Eighty percent of the students were accredited, obtaining between 75 and 95 points out of 100, and receiving a certification issued by the INSP.
The perceptions and evaluations of the course by the students and program coordinators were remarkably positive, recommending that the course be implemented in all health units across Guatemala. Nevertheless, several difficulties were reported by the students. The most important was the difficulty to access computer equipment and the lack of training on the use of computers with internet in remote places. Some students complained about the excess of homework and the little time allocated for it. However, it was a general consensus that the applied benefits generated by the learning process were immediately felt in the technical field work, making the health workers more reassured.
b) Ecological ovillanta. A total of 84 households (from 2204 in the area) were the focus of study within the 7 study site neighbourhoods. All 84 households that agreed to participate in our study were allocated to the study intervention (2 ecological ovillantas and 1 standard ovitrap). Intervention households were no more than 50 metres apart (in three continuous blocks), which we felt gave sufficient coverage because mosquitoes tend to fly up to 500 m around looking for blood or an oviposition site 24 .
Ovillantas were set up on Feb 8, 2015; the monitoring ovitraps were installed only on April 12, 2015. All of the systems, ovillantas and control ovitraps, were monitored and cleaned by the health workers on a weekly basis, with community members present. The health workers filtered and recycled the attractant solution, counting and eliminating any eggs deposited on the landing strips. One interruption of this weekly schedule took place, for 5 weeks in August and September, due to labour and political problems within the Ministry of Health in Guatemala.     However, within the study site households, there were no statistically significant differences in the amount of Aedes eggs collected from ecological ovillantas and standard ovitraps over the 42 week study. We believe this may be because mosquitoes leave a naturalpheromone with each egg each time they oviposit, thus the recycling may have concentrated the natural pheromone (the initial solution was not discarded, but instead new solution was added). Understanding this further will be the focus of a future study.
We also observed that independent of the data obtained per neighbourhood, the amount of . We speculate that there may be a synergistic attraction effect when there are ovillantas present in close proximity to standard ovitraps, which could explain why the cluster of ovillantas/ovitraps produced better results per unit than an ovitrap alone ( Figure 2).
The observed amount of Aedes eggs differed within neighbourhood. There was also the competition of the ovillantas/ovitraps against natural breeding places within a given house, based upon an entomological poll we conducted (Table 2). Among the study neighbourhoods, El Centro stands out as the site with highest egg count across the study period (mean= 47.23; 95% CI: 43.12, 51.34), and major breeding container diversity, with 127 different types (drums: 16.26%, laundry water basin: 21.95%, tubs: 9.76% and other smaller containers: 21.95%, and from each one of the container types: 40%, 37.04%, 16.97% and 22,22%, respectively presented a positive larval/pupae count). El Centro produced 23.58% of the total Aedes larvae found among all the study sites. In the neighbourhood La Esperanza (mean= 41.22) an average of 7.79% of the whole containers contained larvae/pupae. While the same were found among the laundry water basin and buckets/canisters in 12.50% of each type of container. In the neighbourhood of San Miguel 50% of the tires found contained larvae/pupae, followed by 37.50% of laundry water basins. In contrast the neighbourhoods of La Pista and Hojita Verde showed the lowest levels of larvae production among the inspected containers with 4.55% (mean= 4.05) and 4.44% (mean= 0.14), respectively. In both of these neighbourhoods the drums were the main containers where larvae were found (21.05% and 16.67%, respectively).
We were unable to conduct definitive viral identification tests because the technology does not exist, or is inaccessible in these remote locations, and was planed only for Phase II of the project. The low numbers among the official reports (Table 3) show the limitations, in several communities around Sayaxche, of establishing a surveillance of confirmed or suspected cases. Cases are diagnosed only in extreme clinical conditions of fever, rash, etc. Taken together, several themes of sociocultural issues were identified from the focus groups, which can help guide future strategies geared to strengthen community participation in mosquito control: 1. Belief that the mosquitoes breed only in natural ponds, not in backyards.
2. Belief that cleaning of the house and garden are tasks of women only.
3. Belief that the Ministry of Health's services are not efficient.
5. Very little information is provided to the public about the cause of illness and consequences.
6. Dependency of the community on public services to maintain the cleanliness of the streets.
7. Dependency of the community on public antimalarial control, without participation.
8. Low regard for the advantages of community participation.
9. Communication difficulties between some ethnic groups.
10. Low acceptance of new mosquito control methods and transmission prevention strategies, despite wanting less reliance on pesticides.
In terms of the ecological ovillanta as a form of mosquito control, community members provided several views. Overall, the recycling of the attractant solution was welcomed given the difficulty to obtain clean water in the region. There was satisfaction with the use of both the ovillantas and ovitraps as preventive methods and to reduce the overall number of adult mosquitoes. There was interest in knowing how many Aedes eggs were collected each week, and women in particular were keen to be more involved in the activities related to health and showed interest in knowing how the system worked and learning how to maintain it. Among those surveyed who did not have ovillantas, many showed interest in having them installed at their households. In general, the impression was that the ovillantas were effective in reducing adult mosquitoes.
Among the health workers and manager interviewed, the training programme was said to motivate and strengthen their social and technical field work. Financial resources, vehicles, fuel and personnel were said to be scarce. They requested more research in the field, so they could further learn. They expressed concern that implementation of any new strategies be sustained.

Discussion
We found our three-component integrated intervention to have proved beneficial to this remote community at risk of mosquitoborne diseases such as dengue, chikungunya, and Zika. The combination of health worker training, low-cost ecological ovillantas, and community engagement ensured the project met local needs and fostered collaboration and participation of the community, which can help improve sustainability.
The integrated involvement and web-based certification of local health workers strengthened expertise in the area, and has generated evidence of ecosystem alternatives against Aedes mosquitoes.
The ecological ovillantas, made out of recycled material (garbage that when left in the field increases the potential of creating breeding sites), proved positive in the capture of Aedes eggs and possibly on the reduction of the adult mosquito populations, more work needs to be performed, using adult traps, to confirm this. A remarkable acceptance and willingness to participate was observed, not only from the community but also from health workers who monitored its implementation. This was bolstered by community members learning and observing the early biological life stages of the vector, as well as observing the number of Aedes eggs collected in their households, which permitted them to relate those to the presence and quantities of adult mosquitoes potentially produced. It is possible that there may be a synergistic effect between the motivation to participate in the control of mosquitoes, using the innovative strategy of the ovillantas, and the complementary actions needed to maintain an orderly and clean house and backyard. This serves to avoid the creation of artificial breeding sites for the Ae aegypti mosquito and the transmission of viral infections.
Interestingly, a recent report by Ayres 25 from the Brazilian Oswaldo Cruz Foundation described that species of mosquitoes other than Aedes can be infected with the Zika virus, including Culex spp. mosquitoes, in laboratory settings. Recent reporting from Brazil has also affirmed concerns that infection with Zika virus of Culex mosquitoes is vastly more common than Aedes and is being overlooked in the prevention and control efforts 26 . So while the contribution of these other species to Zika transmission has not as yet been firmly established, we anticipate that our ovillanta approach could potentially be used to reduce populations of either species. For example, Culex restuans/pipiens mosquitoes were collected in the first Canadian study using a modified ovitrap. In the summer of 2007, approximately 3.2 million eggs (in rafts) were counted and destroyed in the city of Sudbury, Canada in a 90-day study, using 150 modified ovitraps, resulting in a 90% reduction of the adult Culex spp. population within the study sites (unpublished results Ulibarri et al.). The only requirement to attract a different species, or to have an effect on both Aedes and Culex mosquitoes in the region, is a change of the attractant solution used in the ovillanta, or the set up of a second ovillanta with different attractant solution -the equipment stays the same.
In general, the level of knowledge that community members and health workers held about different viral infections was almost non-existent and, at times, wrong. Clearly there is a need to carry out novel strategies aimed at gaining and maintaining the attention of the community; traditional recommendations provided by health workers tend to bore them, they said. More dynamism is necessary, especially with children. In addition, it is important to include the active participation of infected patients with health centres to avoid further mosquito infection. In this way, infected symptomatic cases can be properly recorded and monitored, and the patient can be followed accordingly.
The participation of the population in vector-borne disease prevention and control is an area that requires more effort and attention. One aspect requiring sensitivity is the clear extent of individualism within communities in this area of Guatemala, and the evident conflicts among different ethnic groups, mainly around culture and language. These work against social cohesion and participation. All the government groups responsible for ensuring health and safety require coordination and collaboration, including reducing the number of abandoned lots where mosquitoes breed. It would be beneficial for the government to apply an ecosystem approach 27 for the communal benefit and to establish a mechanism for people not to throw garbage on the streets and learn to recycle the same properly.
There were several limitations to our study.
Our project was not able to consider the inclusion of the epidemiologic impact of the methodology on viral transmission using sequential polls of seroprevalence. However, the intensity of dengue transmission while the project developed was higher in cities close to Sayaxche, such as Las Cruces and La Libertad. During our study period, the reported incidence of dengue in 2015 for La Libertad, Las Cruces and Sayaxche was 3.33, 10.91 and 2.06 per 10,000 pop., respectively. This was also a direct observation of the Director of the Program in Sayaxche with respect to the buffering effect possibly associated with the intervention using the ovillantas. The Pan American Health Organization (PAHO) reports 18,058 probable cases (1,228 laboratory-confirmed cases) across Guatemala in 2015 28 and 1,179 probable cases up to week 11 of 2016. We ask ourselves, how has the city of Sayaxche been spared this national burden? The confirmed incidence in La Libertad was 5.29 times higher than the ones registered in Sayaxche during the same year. The effectiveness of the ovillanta intervention on the reduction of dengue virus transmission within the city of Sayaxche could be based on the fact that zero autochthonous cases were reported, and only three imported cases were confirmed (unpublished data, Area de Salud Peten-Suroccidental). We remain cautious and will continue to monitor the epidemiology closely.
Second, while we believe the neighbourhoods and households shared the same or similar features in terms of sociodemographics, climate, and others, we did not specifically measure these variables. And although we systematically monitored the presence of backyard containers with larvae or potential water collection (in part through the entomological poll), their presence was not documented nor measured in households that may also have served as mosquito breeding places (i.e., tubs, drums, etc. that filled with rain water).
Third, we did not implement and complete the health worker training prior to initiating the second and third components of the study. The web-based training for health workers was provided simultaneously, showing that the trained personnel gained a better understanding of the vector control process and a better transfer of information to the community. All of the health workers involved in installing and monitoring the ovillantas undertook the web-based training, and only one failed to be accredited. We believe that early training of the personnel, prior to interacting with the community, might have produced better results. The fact that the community were already acquainted with most of the health workers enabled their acceptance of the community engagement activities and of the ovillantas study.
Nonetheless, our project provides evidence for a promising alternative to harmful pesticides and standard ovitraps at a time

Supplementary material (Spanish-language)
Annex 1. Intensive course to strengthen the vigilance, prevention and control of dengue transmission, an ecosystematic approach. (Curso Intensivo para el fortalecimiento de la vigilancia, prevencion y control del dengue con enfoque ecosistemico, 16 pages).
click here to access the data. click here to access the data.
Annex 5. Raw data of the Enthomological Poll.
click here to access the data.

References
when the threat of viral outbreaks is increasing. By incorporating ecology and community-oriented elements, this alternative has the potential to be effectively scaled-up and be sustainable.
Author contributions GU developed the technology. GU, AB and JJR designed the study and implemented the methodology. AB modified, administered and supervised the INSP course and evaluation and performed the statistical analysis. MB designed the community focal groups evaluation and carried out the interviews. JJR managed the health personnel group and collected the data. GU and AB wrote the manuscript. All authors read and approved the final manuscript.
Competing interests GU used to advise the developers of the Green-Strike ovitrap in Canada, and declares that has not received financial compensation for his contributions. All authors declare that they have no competing interests.

Grant information
This research was conducted under the project # 0624-01-10 funded by Grand Challenges Canada through their Stars in Global Health Round 7 programme, Phase I.
The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. I acknowledge the authors' change to the title to reflect that the study is ongoing and the results are preliminary, the publication is certainly a preliminary report. The other reviewers raise some legitimate concerns over the study design and statistical treatment of the data. I felt more could be done in the current version to at least address some of the legitimate concerns over the choice of statistical tests used (e.g. t test and linear regression analysis) and urge the authors to address these concerns to correctly present and comment on the preliminary data they currently have on hand. I also recommend the graphs are improved to attain publication quality (suggest using publication quality graphing software) and that the authors clearly label x and y axes. I struggled to follow when the data represented total counts for all traps combined or were for individual units, for example. I strongly suggest that the experimental design concerns raised by the reviewers are being addressed in the ongoing study so that the authors can confidently measure the trapping improvements made by the ovillantas.

I have read this submission. I believe that I have an appropriate level of expertise to confirm that it is of an acceptable scientific standard, however I have significant reservations, as outlined above.
No competing interests were disclosed.

Competing Interests:
Author Response 27 Jan 2017 , Laurentian University, Canada Gerard Ulibarri Let us not forget that the primary objective was the study of the acceptance, by the community, of the methodology used to collect mosquito eggs, in order to obtain a good and sustainable surveillance and vector reduction. Thus the original design of the study was based on the community acceptance of the intervention solely. and participation The creation and use of the ovillantas was only a secondary necessity, due to the last minute withdrawal of support from a Canadian company, with the originally planned modified ovitraps (which had already been tested in Canada and Mexico, data being prepared for publication). To our surprise, the ovillantas were well accepted by the community, thus the study was labeled a success, according to our initial experimental design. Nevertheless, we recognize that more work needs to be done in the field of community participation.
The use of the ovillantas provided some puzzling results, collecting large amounts of eggs, Aedes The use of the ovillantas provided some puzzling results, collecting large amounts of eggs, Aedes thus we decided to rapidly run a simple test on the null hypothesis of the intervention as a whole. We used the independent simple t-test simply to find out if the average between the control site and the intervention site was significant and statistically meaningful in the short 10 months duration. This simple test has provided us with a positive answer that will allow us to continue expanding, with confidence, on the intervention's approach to community participation/personnel training and modified ovitrap use.
In future studies, with more data and a better designed study on the use of the ovillantas, we plan to use other statistical analysis, such as two-way ANOVA, Mann-Whitney test or another statistical test that allows to corroborate the null hypothesis that the ovillantas are more attractive than the standard ovitrap.
We do not consider necessary to describe these paradigms in the paper, given that only preliminary results are shown. It must be clear to the reviewers that we have not claimed that the ovillantas alone are much better than the standard ovitrap. More data and testing are required to achieve this goal, and this idea is included in our future plans.
We feel that it is not necessary to expand on explaining, at this point, our choice on the use of a simple test to find the statistical significance given the quantity of data recollected so far. We hope this will suffice to the Reviewers to accept that we are still working on obtaining the required data, and that the objective of this preliminary publication has been the sharing of positive results using a tool affordable to everybody, in the midst of a crucial viral dissemination by mosquitoes. This article describes an intervention carried out in rural Guatemala consisting of comparing standard vector control against an integrated delivery of a) web-based health worker training, b) low-cost ovitraps ("ovillantas") and c) community engagement. I agree with the other reviews and add the following: The title is quite long. In the introduction make clearer that the modified ovitraps are called "ovillantes" (the photo shows that but not in the introduction). To what extent do the authors think the ovillantes can be replicated in other settings, e.g., urban? Why did the authors choose to work on in a rural Aedes aegypti area, although is mainly an urban vector? Would ovillantes be applicable in cities, in Aedes aegypti Guatemala or elsewhere in Latin America? Pertinent research project in times of arboviral disease epidemics, however, under methods more clearly describe study design and indicators used to measure intervention effectiveness and detail methods, including methods in qualitative research. There is now growing evidence that community interventions can in fact have an impact on the disease incidence and serological parameters (recent publication on Nicaragua), so there is a need for good research and strong evidence on the effect of community interventions. This project did not do that, clearly spelled out in the discussion section, but the methodology section could be strengthened by explaining the analytical (and not only descriptive) elements of the study design.
The results suggest that ovillantes are more effective than standard ovitraps by comparing both interventions in different neighborhoods ("study sites"). Clarify whether this is by cluster-randomization or not.
Focus groups results are presented to show (positive) impact on social participation but they are not presented by intervention areas so that the effect of the intervention on social participation cannot clearly be attributed.
As the article argues, the FGD results "can help guide future strategies geared to strengthen community participation" but they do not fully indicate an impact of the intervention. Also, while referring to the "community" and social participation the article refers to different ethnic groups and certain "communication difficulties": unclear what this refers to (aren't all of the communities of Mayan descent? What sub-groups or other ethnic groups is the article referring to?).
I have read this submission. I believe that I have an appropriate level of expertise to confirm that it is of an acceptable scientific standard, however I have significant reservations, as outlined above.
No competing interests were disclosed.

, Laurentian University, Canada Gerard Ulibarri
Comments from the authors are accommodated following the reviewers 'paragraphs' Title has been modified. Sayaxche is a small city (urban) in an isolated area of Guatemala (rural). All study was carried out within the city limits. Sayaxche was chosen because the high dengue cases in previous years. Yes, there are plans to implement the ovillantas in other cities, in Latin America, Asia, South Pacific, etc. Wherever the mosquitoes are a problem, some people are already doing so. Aedes This is a publication explaining the preliminary results from a three-prong study, a new publication will deal with the technical parts of the study, when data is more abundant. We know that this publication might have been premature, when dealing with pure scientific data. We trust that publishing these preliminary results early, could help more people given the urgency. We believe that together we could learn more about the implementation of this simple/affordable way to reduce population, and in turn provide tools aimed at protecting vulnerable people Aedes Given the size of the city of Sayaxche, the ovillantas setting was 'randomized' in a manner that most of the city was covered. Although, a slight preference was given to sections of the city where most affected by dengue, based on previous years local data.
The aim of the study was to 'study' social participation at the same time as the effect of the 'new' ovillantas on amount of mosquitoes produced in the area. The social participation was clearly present, the effect of the ovillantas was clearly demonstrated. Now, we need to study the long term effect of the combined methodology.
In this study in particular, we encountered a cultural 'division' among the different members of the community. At times, we required a translator to communicate with the 'participants' of the study, given that their mother language was Mayan, not Spanish. This difficulty might or might no be important, but needs to be considered while implementing these types of strategies in other settings. Cultural values in Mayan culture are different than most Western societies, thus we needed to adapt to this challenge, and the outcome was positive. Overall: Good example of intersectoral engagement and innovation in vector surveillance. The ovillanta design, which the authors show to be a highly sensitive vector monitoring tool, is most important element of this publication. The paper needs to be strengthened through greater review of the literature in the introduction and discussion, clarifying research methods especially social science methods, and clarifying the data shown in the figures. The title is misleading (and some of the text), because the authors do not demonstrate a reduction in abundance. Ae. aegypti demonstrate a reduction in abundance.

Introduction
Paragraph 3. "The rapid spread of mosquitoes… began around 2000." I'm not sure that this is Ae. aegypti correct. I believe that Aedes reinvaded the Americas in the 1970s-80s, and dengue reemerged and spread through the region in the 90s and 2000s. Please check other references.
Paragraph 4. Strengthen the discussion of Aedes surveillance tools. I suggest discussing currently available ae aegypti surveillance techniques, the role of surveillance in disease control, and limitations of current techniques. Are you comparing stationary vector surveillance/monitoring tools, vector control tools, or integrated surveillance and vector control tools? Traditional ovitraps, human landing catches, and bg sentinel traps all have slightly different objectives. Be sure to clarify this. I would suggest refocusing on the role of ovitraps, and conducting a more comprehensive literature review of different ovitraps currently on the market, their limitations and strengths.
I suggest incorporating the paragraph about filtration and recycling into the subsequent paragraph so it is easier for the reader to understand that the current study is based on prior well-developed studies conducted in Canada. This will help with the flow of the intro.
How did you determine the most attracting solution? Lab based experiments? Please explain or cite prior studies.
In the introduction, please clarify the objectives of the study, the study design, and the study endpoints (e.g., increased education of health workers, number of Ae. aegypti eggs in XX communities).

Methods
I was a bit confused by the study design. Consider creating a diagram or map that depicts the study design. Why were standard ovitraps used in the intervention community?
Egg counts are an indirect measure of adult female mosquito abundance (true entomological risk). Egg counts are affected by the number of alternative breeding sites in the environment. Did you account for other factors that could affect egg counts during the study period, such as differences in household characteristics between the treatment and control sites, elimination of containers or other vector control interventions?
Overall the social science research methods and results lack sufficient detail determine the validity of the results. Three interviews is a small sample size. How do you know these are representative? Given the small number of informants, this information could also be included as part of the local context in the discussion section instead of presented as a result. How did you analyze the qualitative data from focus groups and interviews? What social science research methods were used to analyze the transcribed texts? Were the texts coded? Who did the coding? What software was used? Please include appropriate description of the methods and references.
Please provide more detail regarding the entomological surveys. Did you empty and collect and count all larvae/pupae from all containers? How were cisterns managed? Did you conduct the entomological surveys in the control neighborhoods to ensure that there were no differences between treatment and control sites? What dates/season did you conduct the surveys? The container positivity will vary greatly by season.

Results
Can you summarize the results of the training evaluation survey in a table?
Strongly recommend showing summary statistics (maybe from the national census if not available from study households?) to compare the characteristics of the the treatment and control communities or households, to show that there were no major differences in housing conditions, demographics, access to piped water, etc. which could influence differences in the egg count data. In my experience, subtle differences in access to piped water can create completely different vector population dynamics even within the same city.  Please produce publication quality graphics, with consistent formatting and axes (e.g., epi weeks versus months) The investigators report that more eggs were collected from the intervention site with ovillantas. This clearly shows that ovillantas are a more sensitive surveillance tool. I think it is difficult to say whether they are more effective at reducing the mosquito population, unless you have other entomological indicators to show that the mosquito population was suppressed.

Discussion
The investigators indicate that the level of knowledge of community members about viral infections is low. Please show these results in the results section.
Under study limitations, the major limitation (if the authors are claiming that the ovillanta is an effective vector control tool) is the lack of data on adult abundance or other entomological Aedes aegypti indicators.
The investigators should broaden their discussion to discuss their findings in light of other studies published regarding community dengue perception, alternative ovitrap designs that have been published recently by R. Barrera and others, and the effectiveness of community participation in vector surveillance. The discussion and introduction could be strengthened by a deeper review of the literature.

Other comments:
Please include a map of your study site. The authors mention that they limited by lack of climate data. This information is available online. They could download the data and and plot temperature and rainfall with Figure 3, to explain the seasonality.
I have read this submission. I believe that I have an appropriate level of expertise to confirm that it is of an acceptable scientific standard, however I have significant reservations, as outlined above.
No competing interests were disclosed. Competing Interests: engagement activities. The authors are commended on the performance of a mosquito trap made from a common waste item that normally contributes to mosquito production. The new traps, referred to as ovillantas, caught more eggs per site, per device, than standard ovitraps.
Although the title refers to the control of , which is the theme throughout the manuscript, the Ae. aegypti studies presented are not specifically tailored to measuring the success of the components in the context of control (mosquito population reduction). For component one; web-based education of Ae. aegypti health workers; a knowledge, action and practice (KAP) survey of the knowledge of health workers before they undertook training could have assisted to determine the changes due to the training module. For component two; data for the counts of mosquito larvae/pupae in different mosquito traps over time is presented within treatment communities. While this provides promising data on the effectiveness of ovillantas as trapping tools, there are no indication that the trapping had the effect of reducing the mosquito population. Ecological ovillantas may prove to be excellent tools for monitoring mosquito population numbers in resource limited settings, however more work is needed to establish their ability to reduce mosquito population sizes. The authors attempted to asses the effect of their intervention on mosquito control by examining Dengue seroprevalence data, however low case numbers were reported in official records and these are likely to be underestimates, limiting this approach. Adult mosquito trapping would be another avenue to monitoring mosquito population changes. For component three, several themes were identified among socioeconomic issues affecting mosquito control, however future studies will be required to measure resulting improvements in community engagement.
In summary, a multi-component strategy towards the control of is presented that incorporates Ae. aegypti training of health workers in vector control, a new mosquito ovitrap constructed from recycled materials and community engagement activities. While an increase in trapping efficacy is demonstrated for the ovillantas, it is difficult to gauge the effectiveness of these components on control from this Ae. aegypti study alone. The title and theme may be better presented as "development of strategies the towards control of ". I am hopeful that improved control can be demonstrated from future Ae. aegypti Ae. aegypti application of these interventions.
I have read this submission. I believe that I have an appropriate level of expertise to confirm that it is of an acceptable scientific standard, however I have significant reservations, as outlined above.
No competing interests were disclosed.

Competing Interests:
Author Response 30 Nov 2016 , Laurentian University, Canada Gerard Ulibarri Although we tend to agree with the Mr. Hugo (reviewer),we clarify that this is a report on preliminary data. Entomological studies are necessary in order to establish with certainty that the viral infection has been reduced. Adult traps do not work very well in spp. mosquitoes Aedes (reason for the use of standard ovitraps to monitor the presence of females in the study Aedes area). Nevertheless adult traps are planned for the near future studies, something very difficult to achieve in remote communities where commercial carbon dioxide is not easy to access. A new trap is being designed to overcome this difficulties, and results will be reported in due time.
Title has been modified to express that these are 'preliminary results", more detailed In summary; data will be provided in due course N/A Competing Interests: Title and Abstract: I think these are both a little misleading. The integrated intervention was not evaluated in such a way that would allow for rigorous evaluation (there were too few trial arms) -I think the presented results can only be used to compare the two different trap types. The sentence in the abstract that indicates the higher mosquito egg collections found using the novel trap is also misleadinganalysing total egg counts over the course of the experiment actually indicated higher captures with the standard ovitrap.
There are major issues with the study design. Not least of all, it does not allow for the Article content: assessment of any additive benefits from community engagement and healthcare worker education. The authors tested their new 'ovillantas' trap against standard ovitraps. Unfortunately, they only used lure-baited water in their novel trap meaning any difference found between trap types could just as easily be a result of the lure as it was the new trap design. Although cost was mentioned several times in the study, no indication was provided of the relative costs of the new tested trap (plus lure and construction time) versus the standard trap and so no conclusions could be drawn pertaining to cost effectiveness.
Recycling the water in the traps is a good idea in the short term because natural chemicals emitted by ovipositing mosquitoes are attractive to future ovipositors. However, there must be an optimum duration over which water can be recycled, beyond which the build-up of algae etc would actually be unattractive to ovipositing females. This was never mentioned.
Lack of internet access in this remote population is something that perhaps should have been identified as a major study limitation before the attempted use of web-based education services.
After community participation inevitably wanes and the eggs are no longer collected and routinely removed/destroyed, the ovillantas will provide ideal breeding grounds for disease vectors. Part of the study involved community education on eliminating breeding grounds from participant houses but these 'traps' sound as though they themselves will become ideal larval sites in time. The only ways around this would be the removal of traps by trained personnel over a predetermined time period or the continual surveillance of the local communities' upkeep of the traps -both expensive and unlikely to occur. Count data were analysed using t-tests. Count data are typically not normally distributed (typically they are Poisson or negative/binomial distributed) and would require alternative analysis methods.
Minor issues in the content include the assertion that ovitraps have previously been shown to significantly reduce adult mosquito numbers -to the best of my knowledge this is not the case. Also, the authors mention that the 500m dispersal ability of informed the distribution of the intervention A. aegypti households (no more than 50 metres apart) but I neither understand this logic nor agree with this very-much-upper-estimate in mosquito movement.

A. aegypti
Overall, I'm left with questions over the usefulness of this new tool relative to the standard Conclusions: trap -depending on how the authors analysed their data they showed improved captures with both traps trap -depending on how the authors analysed their data they showed improved captures with both traps (and neither analysis was conducted rigorously). I also am left unsure of the additional benefits in terms of community participation or education/training because the study design did not allow for these to be tested independently.
The data appear to be presented clearly and are in a format that allows for their future use by other Data: scientists.
I have read this submission. I believe that I have an appropriate level of expertise to confirm that it is of an acceptable scientific standard, however I have significant reservations, as outlined above.
No competing interests were disclosed.

Competing Interests:
Author Response 30 Nov 2016 , Laurentian University, Canada Gerard Ulibarri ;The title has been modified to clarify that these are preliminary results shared Title and Abstract with the public due to the Zika presence. More trials are planned when funding is granted. In the mean time, this is an approach which is working and should be used to help people protect themselves. One of the novelties here was that people from the community did participate actively, using a novel approach made out of items from their backyard. Higher mosquito egg count was achieved on site with ovillantas against site with only standard ovitrap. Although, the reviewer is right that the ovitrap in ovillanta site collected more than the ovitrap alone. We are investigating this phenomenon and believe is due to the 'skip oviposition' nature of the mosquito.

Aedes
; We also compared water with ovillantas, against standard ovitraps, and at all time Article content the ovillantas collected about 2.5 times more eggs than standard ovillanta (data not shown). We did find algae problem but in very few ovillantas. The fact of filtering and recycling the solution seemed to keep the solution clean for extended period of time. When an ovillanta was polluted, then the whole solution was replaced with fresh water plus attractant solution.
Internet accessibility at the Health Center was identified prior to implementation of the study. What was a problem, and we did not see it at the beginning, was that not everybody has access to internet in their homes, and internet is extremely slow in the region. Thus, it was necessary that every single person travel to the Health Center for the 'lessons', an inconvenience to those far from the Center.
; Absolutely, the same would happen if standard ovitraps were used.

Community participation
For this reason, the Health personnel was trained to supervise the surveillance and cleanliness of the ovillantas in a regular basis while gathering the information on egg count (pellon paper exchange). If the participant failed to maintain the ovillanta, this was removed from their premises or the Health personnel took over the maintenance. Very few instances of this happened. t-tests; Other methods will be used and compared when more data is available. For the time being, we considered that the t-tests were enough to show the significant difference provided by the use of ovillantas against standard ovitraps.
: This has been a very difficult, three-prong, study, and the paper reflects the first Conclusions : This has been a very difficult, three-prong, study, and the paper reflects the first Conclusions data produced, more will be produced in due time. The Health personnel showed improvement after their Internet-based course, the community accepted the ovillantas very well and participated (something new), and the cluster of ovillantas collected more eggs than the isolated standard ovitraps. Those are the facts. We are still studying how each one of the interventions influenced the outcome of the whole intervention. In this report, we have presented the results to the holistic approach. More questions will be answered and data will be presented in due time N/A Competing Interests: