Effect of PVP on the characteristic of modified membranes made from waste PET bottles for humic acid removal

Background: The aim of the present study was to evaluate the possibility of using recycled polymer (waste polyethylene terephthalate [PET] bottles) as a membrane material. Furthermore, the effect of the addition of a pore-forming agent and preparation conditions was also observed. Methods: Porous polymeric membranes were prepared via thermally induced phase separation by dissolving recycled PET in phenol. PET polymer was obtained from waste plastic bottles as a new source of polymeric material. For original PET membrane, the casting solution was prepared by dissolving of 20wt% PET in phenol solution. For PET modified membrane, a 5 wt% of polyvinylpyrrolidone (PVP) was added into polymer solution. The solution was cast onto a glass plate at room temperature followed by evaporation before the solidification process. The membranes formed were characterized in terms of morphology, chemical group, and filtration performance. A humic acid solution was used to identify the permeability and the solute rejection of the membranes. Results: The results showed that the recycled PET from waste plastic bottles was applicable to use as a membrane material for a water treatment process. The maximum flux of 97.0 l/m 2.hr was obtained from filtration test using PET membrane. The highest rejection of humic acid in a water sample, which reached up to 75.92%, was obtained using the PET/PVP membrane. Conclusions: The recycled PET from waste bottles was successfully used to prepare porous membrane. The membrane was modified by the addition of PVP as a membrane modifying agent. SEM analysis confirmed that the original PET membrane has a rough and large pore structure. The addition of PVP improved the pore density with a narrow pore structure. The PET/PVP membrane conditioned with evaporation was the best in humic acid rejection.


Introduction
Clean water is one of the most vital and essential elements for sustaining human life. This is the reason why the lack of drinking water has become a serious issue for the entire world 1,2 . Membrane technology has been applied widely in water and wastewater treatment processes. In water purification, organic material contaminants, such as humic acid and suspended solids, are effectively removed by microfiltration or ultrafiltration membranes 3 . The advantages of separation using membrane technology are that it is free of chemicals or additives, uses little temperature or at least less energy compared with conventional treatments (i.e. coagulation followed by sand filter), and that it is scalable and hybrid-separated 4 .
The effectiveness of the ultrafiltration process using a membrane depends on the material and preparation process. Membranes are prepared from organic substances, such as polymers, or inorganic and composite materials. The polymeric materials generally used in membrane fabrication are cellulose acetate (CA), polyethersulfone, polyvinylidene fluoride, and polyethylene terephthalate (PET). PET is commonly used for membrane ultrafiltration in the separation process. Khayet et al. used PET membrane grafting with polystyrene for methanol/toluene separation through pervaporation 5 , while Behary et al. conducted bio-separation from surfactant using PET membrane modified with chitosan 6 . Li et al. used cellulose acetate with PET as an additive for the forward osmosis process 7 . The effect of PET as an additive is that it increases the mechanical properties of the membrane 7 .
PET is a polymeric material that is generally derived from commercial polymer, which can increase the production costs. However, plastic bottles as drink packaging are composed of PET); therefore, waste plastic bottles can potentially be used as a membrane material 8 . This new source of polymeric material helps to reduce the waste of plastic bottles and constitutes a green alternative to limit the consumption of polymers. Additionally, the use of PET bottles as polymeric material will reduce the cost of manufacturing membranes.
The synthesis of PET membranes from plastic bottles was investigated previously by Rajesh and Murti 9 . Their results showed that PET membranes without modification with polyethylene glycol have poor mechanical properties. Another study by Zander et al. investigated using PET from waste bottles to fabricate fiber membranes via the electrospinning technique. The obtained membrane was used for water filtration to separate latex beads. The study found that about 99% of the beads can be removed from a water sample 10 . In the water treatment process, the pore size of the membrane has an important role in the rejection of water contaminants. Membranes with a small pore size, but high pore density, is recommended to obtain a stable permeation with high selectivity of water contaminant In this study, the PET membrane from plastic bottles was modified by addition of PVP to enhance its pore size and pore density. The addition of PVP as a pore forming agent and evaporation on the casting film may affect the quality of the resulting membrane. This ultrafiltration PET membrane was then investigated for humic acid removal, and its characterization was performed using the water permeability test, scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), and ultravioletvisible (UV-Vis) analysis.

Materials
PET was derived from waste plastic bottles. Phenol was used as a solvent (KGaA Merck, Germany). PVP (40,000 Da) was purchased from Sigma-Aldrich Co. Ltd (USA). Humic acid (HA) powder was also obtained from Sigma-Aldrich. The HA solution was synthesized by dissolving HA powder in 1 L of distilled water.

Preparation of the PET membrane
The membrane was prepared via thermally induced phase separation (TIPS). Firstly, phenol, as a solvent, was heated at 50°C until the liquid phase was reached. Fragments of PET bottles were dissolved in molten phenol at 100°C and stirred using a magnetic stirrer for 6 hours until homogeneous. In order to improve the performance of the membrane, 5wt of PVP was added to the solution. Four types of membranes were composed: the composition and the condition of each dope solution are summarized in Table 1. A minimum of four membranes were made of each type, and three membranes of each type were chosen for the filtration experiments (below).
After obtaining a homogeneous solution, the dope temperature was maintained at 100°C without any stirring to remove air bubbles. The homogeneous solutions were cast uniformly onto a glass plate using a Baker applicator (YBA-3, Yoshimitsu, Japan) at room temperature. The thickness of the membrane was set at 700 µm. The casting film was left in the air for 7 minutes to evaporate the solvent. The glass plate was then dipped into a coagulation bath containing water-propanol 1:12 as a non-solvent.

Amendments from Version 1
This is the revised manuscript based on referee comments. In this manuscript, some part have been changed, i.e. the PET concentration data shown in Table 1, the symbol remark style for Equation (2), and replacing Figure 8 with a simpler two-dimension scatter plot.

REVISED
Another casting film, for no evaporation treatment, was directly immersed into non-solvent. The glass plate was then dipped into a coagulation bath containing water-propanol 1:12 as a non-solvent. The membrane sheets formed were washed and stored in distilled water for 1 day to remove any residual solvent.

Membrane morphology
The morphologies of the membrane surface and cross-section were analyzed using a scanning electron microscope (model, JSM 6360LA; JEOL Ltd., Japan). The dried sheets of membrane were gold sputtered for producing electric conductivity. Photomicrographs of PET membranes were viewed in vacuum condition at 5 kV. The magnification image was taken at 10,000 x for the surface and 700 x for the cross-section.

FTIR spectra
Functional groups of the membrane were analyzed using a Shimadzu FTIR-8400 spectrometer (Japan). A wavelength of 4000-400 cm −1 was used, and the chemical groups of the membranes were identified by their peaks using IR solution 1.50 software (Shimadzu).

Filtration performance
The ultrafiltration test was conducted using dead-end filtration and pressurized with nitrogen gas. The filtration area of the membranes was 15.2 cm 2 . The operating condition was set at 1 bar transmembrane pressure and room temperature. Filtration was carried out for 30 minutes, and the permeate was collected three times every 10 minutes. Three repeats for each membrane type was performed. The filtration experiment was evaluated in term of flux and rejection of HA solution. Flux is the total volume of permeate pass through the membrane in a determined filtration period calculated by Equation (1). Rejection is the amount of HA particle rejected by membrane, as analyzed by Equation (2).
In which: V = Volume of permeate (L) The model for the ultrafiltration test in this study was HA solution at 10 mg/L concentration. The solution was prepared by dissolving HA powder in 1 L distilled water. The rejection value of the membrane in HA filtration was calculated as follows 11,12 : In which: R = rejection (%) The HA concentration in the feed and permeate solution were measured using a UV-Vis spectrometer (model UV-1700; Shimadzu) at 490 nm wavelength. The HA filtration schematic using a PET membrane can be seen in Figure 1.

Membrane morphology
The PET membrane was prepared via TIPS. The constructed membranes were categorized as asymmetric ultrafiltration membrane.
The top surface and cross-sectional images of the membranes are shown in Figure 2 and Figure 3, respectively. Figure 2 shows the changes in membrane morphology with the addition of PVP and evaporation on casting film. PET-2 and PET-4 were evaporated for 7 minutes before being immersed in a coagulation bath. The membranes formed showed a decrease in the membrane porosity. A high evaporation temperature (100°C) for 7 minutes increased the exchange rate of solvent from the surface of the casting film. This led to a higher polymer concentration near the top surface, and, therefore, the phase exchange of solvent and nonsolvent in the coagulation bath became lower. This is called delay demixing, which causes the membrane to have a less porous structure 13 .
The cross-sectional image in Figure 3 shows the asymmetric structure of the sub-layer membrane. The evaporated membranes had a thicker, dense top layer due to the delay demixing. In the sub-layer of the modified membrane, the porous structure changed with the addition of PVP. The effect of PVP in the casting solution   caused the formation of pores and sponge structure in the sub-layer 9 .
In the membrane modification process, enhancing the pore density with uniform pore size is essential. A sponge structure, like the one formed in the modified membrane, affects the filtration quality and mechanical properties of the membrane 2 .

Membrane functional groups
The FTIR spectrum was analyzed to determine the changes of the chemical groups on the membrane surface 3 . Regarding polymer composition in this research, FTIR analysis was carried out for PET-1 and PET-3 membranes only. Polymer composition of PET-2 and PET-4 membranes was similar with PET-1 and PET-3, respectively; the IR spectra of PET-2 and PET-4 are equal to the IR spectra of PET-1 and PET-3, respectively. Figure 4 shows the FTIR spectrum of the PET-1 membrane. A peak of 3630-3300 cm -1 indicated the presence of the alcohol functional group (OH). In the range of 3200-3000 cm -1 and 1700 cm -1 , bands of OH and CO were derived from the carboxylic acid functional group. An aromatic functional group of C = CC band was located at 1650-1600 cm -1 . At 1400 cm -1 and 860 cm -1 , a C-H band of alkanes and aromatics were observed. A very weak peak at 1250 cm -1 indicated a C-O band of the phenol functional group; phenol is composed of the aromatic ring and OH groups 14 . The identification of the membrane chemical groups is presented in Table 2. The chemical structure of PET, composed of several chemical groups, is shown in Figure 5. According to the data shown in Table 2, the membrane was composed of PET material.
The comparison of the FTIR spectrum analysis between PET-1 and modified membranes (PET-3) can be seen in Figure 6. Generally, the FTIR spectrums of PET-1 and modified PET/PVP membranes were similar, because both membranes were made with    The differences in HA rejection in Figure 8 show the influence of PVP as an additive material and the evaporation time on the performance of the membrane. Figure 8 also shows the flux of the HA filtration. The membrane with smaller and uniform pores (PET-4) was better in HA rejection, but produced less permeates (the addition of PVP in the membrane solution increased the total concentration of the polymer and led the membrane to have smaller pores). Additionally, PVP improved the hydrophilic nature of the membrane surface. This prevented the hydrophobic HA molecules from getting closer to the membrane surface 3 . Therefore, the PET/ PVP-modified membrane followed by evaporation (PET-4) was the best at HA rejection compared to the original PET membrane without evaporation (PET-1), or original PET membrane with evaporation process (PET-2).

Conclusions
Membranes with pore structure were successfully fabricated using recycled PET from waste plastic bottles. The characteristics and performance of these membranes were affected by the membrane preparation conditions. In this study, PET membranes were modified by the addition of additives (PVP) and conditioned using evaporation during solidification. Based on the results, it can be concluded that the presence of PVP in polymer system has an effect on the pore structure and flux of PET membrane. The original PET membrane (PET-1; no PVP or evaporation) had a rough pore structure, which resulted in low solute rejection. The addition of PVP improved pore density with a narrow pore structure, and using a high temperature of evaporation resulted in a membrane surface with smaller pores. Consequently, a PET/PVP membrane conditioned with evaporation (PET-4) was most efficient in humic acid rejection. In general the membranes were suitable for use in a water treatment process. Modifying agents of PET membranes should be further developed to enhance the performance of PET membranes, especially for ultrafiltration process.  PET as the basic material. However, a lower peak at 1820 cm -1 and 1180 cm -1 of the modified membrane (PET-3) indicated a carbonyl functional group and CN band. The existence of these bands showed that the membrane was composed of PVP. The molecular structure of PVP is shown in Figure 7. Filtration performance Water filtration is related to membrane characteristics, such as hydrophilicity and pore size. Furthermore, the addition of membrane modifying agent and the evaporation process also affects water permeability 13 . In this study, a feed solution of humic acid (HA) was tested at 10 mg/L. The concentration of HA solution in the feed and permeate were measured using a UV-Vis spectrometer. The comparison of the original and modified PET membranes in the HA flux and rejection is given in Figure 8. According to Figure 8, the PET/PVP modified membrane with evaporation (PET-4) had the highest HA rejection of up to Author contributions NA designed the research proposal, provided experimental material and apparatus, and approved the final draft of manuscript. AA, SA and RS were responsible for conducting the experiment and preparing draft paper. SM contributed to the research report as supervisor.

Competing interests
No competing interests were disclosed.

Grant information
The author(s) declared that no grants were involved in supporting this work.

Department of Chemical Engineering, University of Riau, Pekanbaru, Indonesia
In the discussion about filtration performance (rejection and flux), give more reasons regarding the best performance of PET-4. Make sure that these reasons are related to the characteristic of the membranes. The statistical analysis and its interpretation are only partly appropriate -was the filtration performance conducted on different conditions, like pressure and the temperature of the process?
Is the work clearly and accurately presented and does it cite the current literature? Yes

If applicable, is the statistical analysis and its interpretation appropriate? Partly
Are all the source data underlying the results available to ensure full reproducibility? Yes

Are the conclusions drawn adequately supported by the results? Yes
No competing interests were disclosed.

Competing Interests:
Referee Expertise: Materials characterization I have read this submission. I believe that I have an appropriate level of expertise to confirm that it is of an acceptable scientific standard, however I have significant reservations, as outlined above.
Author Response 14 Jun 2017 , Syiah Kuala Uiversity, Indonesia Nasrul Arahman According to the SEM image in Figure 2, PET-4 has densest and smallest pore than other membranes. The pore can retain humid acid molecules which has larger pore than the membrane. So, in filtration test, PET-4 has the highest rejection of humid acid. Different pressures were conducted in preliminary permeation test. In case of PET-4 membrane, a maximum 3 atm pressure is applied for permeation test. On the other hand, PET membrane without addition of PVP (PET-1, and PET-3), the permeation test only capable at 1 atm of applied pressure. The addition of PVP brought about the enhance of membrane strength so that it is appropriate to conduct the filtration process at pressure of 2 or 3 atm. This is the reason the filtration performance test was carried out at constant pressure at 1 bar for all membrane. Addition of PVP into polymer solution also influence to increase the hydrophilicity of the membrane. Good hydrophilicity of membrane surface can prevent HA molecules attach to the membrane surface. So, we concluded that PET-4 is the best prevent HA molecules attach to the membrane surface. So, we concluded that PET-4 is the best membrane in this study. This study assesses the possibility of using recycled polyethylene terephthalate from used bottle waste as a membrane material. A topic that highly relevant in waste-to-value perspective. The fact that the study was a success make this report worthy of publication. Moreover, the approach of using recycled PET as membrane material is rather new with only few references available. I believe that by addressing comments below, the quality of the manuscript will be improved.
Major comment: When discussing the membrane formation mechanism, the authors mentioned the roles of evaporation time. However, by looking into the properties of phenol (melting point of 40.5 °C and boiling point of 181.7°C ), the rate of phenol evaporation is too low. As seen in Figure 4, phenol in the presence of residue in the membrane matrices is obvious. Another aspect to be discussed is the effect of falling temperature on the solution (I assumed that the casting was done at room temperature) as well as inhibition of water from humid air. Those phenomena occur simultaneously and contribute to the formation of membrane structure.
The authors are expected to address minor comments below and fit their answer into the revised manuscript.
Add information about the permeability in the abstract.
Please revise typo error far description of parameters in Eq. 2: C and C instead of Cp and Cf.
What is the solubility of phenol in a mixture of 1:12 of water:propanol. Is there any preliminary study on selecting this ratio? What will be the impact of non-solvent composition on the produced PET membrane? Figure 8: A simpler scatter plot will be more informative rather than overlapping 3D plots in the current version of the manuscript. Also, information on the testing pressure should be included in the caption.

Is the study design appropriate and is the work technically sound? Yes
Are sufficient details of methods and analysis provided to allow replication by others?

Partly p f
Are sufficient details of methods and analysis provided to allow replication by others?

If applicable, is the statistical analysis and its interpretation appropriate? Yes
Are all the source data underlying the results available to ensure full reproducibility? Yes When discussing the membrane formation mechanism, the authors mentioned the roles of evaporation time. However, by looking into the properties of phenol (melting point of 40.5 °C and boiling point of 181.7 °C), the rate of phenol evaporation is too low. As seen in Figure 4, phenol in the presence of residue in the membrane matrices is obvious. Another aspect to be discussed is the effect of falling temperature on the solution (I assumed that the casting was done at room temperature) as well as inhibition of water from humid air. Those phenomena occur simultaneously and contribute to the formation of membrane structure.
Answer: Thank you for your valuable discussion. We agree with your opinion. Evaporation treatment is one the affected parameter to the membrane morphology. Another parameter as you pointed was also affected to the membrane formation. The changing of solution temperature suddenly from hot plate condition to glass plate at room temperature may affect the membrane formation. However, we did not conduct such kind of this investigation in this study.

Comment 2.
Add information about the permeability in the abstract (revised manuscript).
Answer: Flux information added in the abstract  What is the solubility of phenol in a mixture of 1:12 of water : propanol. Is there any preliminary study on selecting this ratio? What will be the impact of non-solvent composition on the produced PET membrane? Answer: The composition of phenol-propanol (1:12) as non-solvent was determined by conducting several solidification process using a different concentration of water-propanol. The mixture was tested at ratio 1:0; 1:1; 1:5; 1:7; 1:9; and 1:12. The result shows that non-solvent without propanol produced a rigid and kinked membrane with many big holes on the surfaces. The addition of propanol helps to smoothen the surface. But, too much propanol caused mechanical properties of membrane too fragile. So based on the preliminary study, the mixture of 1:12 of water-propanol was the best non-solvent composition to produced PET membrane with good mechanical properties and pore performance.
The solubility of phenol based on material safety data sheet from sciencelab.com : Easily soluble in methanol, diethyl ether. Soluble in cold water, acetone. Solubility in water: 1g/15 ml water. Soluble in benzene. Very soluble in alcohol, chloroform, glycerol, petroleum, carbon disulfide, volatile and fixed oils, aqueous alkali hydroxides, carbon tetrachloride, acetic acid, liquid sulfur dioxide. Almost insoluble in petroleum ether. Miscible in acetone. Sparingly soluble in mineral oil. So, phenol has good solubility in a mixture of water-propanol.
Comment 5. Figure 8: A simpler scatter plot will be more informative rather than overlapping 3D plots in the current version of the manuscript. Also, information on the testing pressure should be included in the caption.
Answer: New figure prepared and replaced for Figure 8 (revised manuscript).
No competing interests were disclosed Competing Interests: