Caucasian and south Asian men show equivalent improvements in surrogate biomarkers of cardiovascular and metabolic health following 6-weeks of supervised resistance training

Background: The South Asian population have greater cardiovascular risk than their age-matched Caucasian counterparts, characterized by unfavorable biomarkers. South Asians may also be partially resistant to the pleiotropic benefits of physical activity on cardiovascular health. There is a current absence of studies that compare markers of cardio-metabolic health between Caucasians and South Asians employing resistance exercise. This study set out to compare the response in biomarkers of cardio-metabolic health in Caucasians and South Asians in response to resistance exercise. Methods: Caucasian (n=15, 25.5 ± 4.8 yrs) and South Asian (n=13, 25.4 ± 7.0 yrs) males completed a 6-week progressive resistance exercise protocol. Fasting blood glucose, insulin, and their product insulin resistance (HOMA-IR), triglycerides (TRIGS), low density lipoprotein (LDL), high density lipoprotein (HDL), total cholesterol (TC), vascular endothelial growth factor (VEGF), asymmetric dimythylarginine (ADMA), L-arginine (L-ARG) and C-reactive protein (CRP) were established at baseline and following resistance exercise. Results: There were significant improvements in fasting glucose, TC, LDL, HDL and VEGF in both groups following resistance exercise ( p<0.05, for all). No change was observed in insulin, HOMA-IR, TRIGS, ADMA, L-ARG following resistance exercise ( p>0.05, in both groups). CRP increased in the South Asian group ( p<0.05) but not the Caucasian group ( p>0.05) Conclusions: The cardio-metabolic response to resistance exercise is comparable in young Caucasian and South Asian males though inflammatory response to exercise may be prolonged in South Asians.


Introduction
Diseases that centre on the cardiovascular (CVD) and glycolytic systems are primary contributants to annual global mortality, accounting for approximately 17.7 million and 1.6 million deaths in 2015, respectively (WHO, 2017a;WHO, 2017b). However, there is considerable heterogeneity of CVD prevalence across racial groups, where the immigrant South Asian (SA) community of the United Kingdom have an approximate 50-100% elevation in cardio-metabolic risk compared with the general population (Wild et al., 2007). This translates to an estimated 5.3 year earlier occurrence of CVD in SAs when compared with their Caucasian (CAUC) counterparts (Hughes et al., 1989).
Pronounced CVD risk amongst SAs is due in part, to the presence of consistently augmented traditional risk factors. For instance, unfavourable lipid profiles been reported in SAs in comparison to CAUCs (Misra & Khurana, 2011), due to inherent variation of lipid particles. SAs exhibit smaller low density lipoprotein (LDL) particles and dysfunctional high density lipoprotein (HDL) particles compared with CAUCs (Dodani, 2008;Tziomalos et al., 2008). Premature development of Type 2 diabetes (and associated complications) occur approximately 10 years earlier in SAs than CAUCs, and data from largescale studies such as United Kingdom Asian Diabetes Study (UKADS) reported diabetes related deaths in SAs occurring 7.4 years earlier than CAUCs (Bellary et al., 2010). Comparably higher systemic C-reactive protein (CRP) with regional obesity of the abdomen is another aspect of cardio-metabolic disease that contrives to increase CVD in SAs when compared with CAUCs. The latter has required revisions in the 'normal' BMI thresholds for overweight and obese SAs (Gray et al., 2011). These racially specific reclassifications are based on data demonstrating that comparable levels of glycaemia and lipid profiles are seen at BMI levels of 21-26 kg/m 2 in SAs compared to 30 kg/m 2 in CAUCs (Gray et al., 2011).
Physical exercise is a well-known prophylactic for the development of CVD. In this respect, the cardio protective effects of aerobic exercise are well documented in comparison to fewer studies of resistance exercise (RES). RES has been shown to promote healthogenic effects by improving lipid profiles (Kelley & Kelley, 2009;Sheikholeslami Vatani et al., 2011), insulin sensitivity (Mann et al., 2014;Schwingshackl et al., 2014), and CRP levels (Donges et al., 2010). However, these studies almost exclusively enrol CAUC participants with the result that there is a general paucity of RES studies and resultant data amongst the SA population.
Few investigations have included SA participants and have used lifestyle modification methodologies which have produced modest results. For instance, a 3-year lifestyle intervention, promoting beneficial dietary and physical activity behaviours in SAs report trivial effects in body mass and no change in either blood pressure or fasting blood glucose (Bhopal et al., 2014). Other investigations that have studied the interaction between physical activity and cardio-metabolic risk in SAs have also found encouraging results. Data from the Health Survey of England show an inverse association between the participation of 30 minutes of moderate intensity exercise per week and cardio-metabolic risk in CAUCs and SAs (Williams et al., 2011). However, findings from the Indian Diabetes Prevention Programme and the ADDITION-Leicester study identified a blunted response in cardio-metabolic biomarkers to physical activity in the SA cohort compared with CAUCs (Yates et al., 2010), which corroborates the findings from other racial comparison studies (Fretts et al., 2009;Steinbrecher et al., 2012). Recently, IIiodromiti and colleagues (2016) have made a valuable addition to the literature by offering that SAs would require ~50% greater participation in physical activity than CAUCs (232 min/week -1 vs 150 min/week -1 in CAUCs) to address the imbalance in healthogenenic benefits of aerobic exercise between these racial groups.
While these data may have potentially important consequences for exercise prescription for SAs, the field is limited by the dearth of objective assessment of exercise participation. Our previous work has demonstrated differences in muscular strength adaptation in response to supervised RES in SAs, which may translate to an attenuated cardio-metabolic response (Knox et al., 2017). These findings are in line with two previous studies which compared objectively determined physical activity (Iliodromiti et al., 2016) and aerobic exercise (Hall et al., 2010) which support the potential for a blunted response to physical activity in SAs compared with CAUCs. However, no study has explored this phenomenon using the medium RES. Therefore, the purpose of this study was to assess whether the different response of exercise in the SA population suggested by previous reports is applicable to RES. We will address this aim by establishing the response of traditional cardiometabolic biomarkers to 6-weeks progressive RES between young, healthy CAUC and SA males. We hypothesised that 1) differences in biomarkers of cardio-metabolic health are evident at baseline (PRE) between CAUCs and SAs, 2) differences in biochemical markers in both groups following RES (POST), 3) both groups differ at POST in any biochemical marker of cardio-metabolic health.

Participants
This study was carried out in accordance with the recommendations of the University of the West of Scotland. The protocol was approved by the School of Science and Sport ethics committee (approval: HREC_Sci2013/02/Knox). All subjects gave written informed consent in accordance with the Declaration of Helsinki. In the present study, the main outcome measure was squat performance. All participants were recruited by local advertisement and word of mouth. Inclusion criteria were that (i) participants did not engage in any recreational or competitive sports, (ii) were naive to RES prior to study enrolment, (iii) no history of cardio-metabolic disease prior to study participation (iv) or any

Amendments from Version 1
There are a few changes in grammar within the new version of the article. Additional information has been added to the 'Participants' and 'Muscular strength and resistance exercise protocol' sections, as requested by reviewers.

REVISED
illness that may provide a contraindication to exercise participation. Only male participants were recruited to control for the effect of sex on potential responses. Race and familial generation of United Kingdom (UK) patriotage was self-reported.

Anthropometrics
All anthropometric measurements were performed by AK in the Human Performance Laboratory of the University of the West of Scotland (HPLUWS). Height was determined using a portable stadiometer (Leicester Height Measure, Seca, Birmingham, U.K.). Body mass was measured to the nearest 0.1 kg using commercially available scales (TBF-300, Tanita, Tokyo, Japan), where participants were required to remove footwear and unnecessary clothing before measurement which were obtained in adherence with manufacturers guidelines. Body mass index was calculated using the following formula; BMI = body mass (kg) ÷ height (m) 2 . Total body fat percentage (%) was calculated using bioelectrical impedance analysis (BIA) using a commercially available analyser (body composition analyser TBF-300, Tanita, Tokyo, Japan). Criterion validity of this method has been suitably comparable (r = 0.952) compared with the gold standard dual energy x-ray absorptiometry (DEXA) (Bozkirli et al., 2007).
Waist circumference (WC) and waist-hip ratio (WHR) was calculated according to the guideline published by the WHO (WHO, 2008) using a commercially available ergonomic circumference measuring tape (Seca 201, Seca, Birmingham). Participants were asked to remove any clothing on their upper body before standing with their arms by their side with their feet positioned close together with their body weight distributed evenly across both feet. For WC, the tape was placed around the approximate midpoint between the lower palpable rib and the top of the iliac crest (WHO, 2008). The participant was then asked to relax and take several deep breaths to account for any abdominal tension caused by the nature of this procedure where criterion measurement was taken at the end of normal expiration to control for any diaphragm movements. Hip circumference (HC) was calculated by measuring the circumference of the widest portion of the buttocks.

Systemic blood pressure
Systemic blood pressure data was collected by AK in the HPLUWS. Arterial blood pressure (BP) was measured by an automated BP monitor (M6, Omron, Milton Keynes, U.K.). Participants sat in a quiet room for 10 minutes with their legs uncrossed and with the arm being used for measurement supported approximately level with the heart. A total of 3 readings of systolic blood pressure (SBP) and diastolic blood pressure (DBP) were recorded. The final data was calculated from the mean values of the three reading. From these values, it was possible to calculate mean arterial pressure (MAP) the following formula; MAP = 0.33(SBP-DBP) + DBP.

Blood biochemistry
All blood sampling and analysis was performed by AK in the HPLUWS. To account for diurnal variation in blood variables, participant blood was sampled at the same time of day and following a 12-hour fast. Briefly, each participant remained in the supine position for at least 30 minutes during sample collection enabling control of plasma volume shifts (Hagan et al., 1978). Venous blood was collected from the antecubital vein via venepuncture. Blood glucose, triglycerides (TRIGS), HDL and total cholesterol (TC) were determined by spectrophotometery (Rx Monza, Randox Laboratories, UK). Samples were mixed with respective reagents and incubated at room temperature for the duration stated by reagent manufacturer (Randox Laboratories, UK) before being inserted into the spectrophotometer and read at the corresponding wavelength. LDL concentrations were established indirectly by the following Friedwalde equation (Friedewald et al., 1972); LDL = TC -HDL -(TRIGS/5). This method was appropriate as all samples did not exceed fasting TRIGS concentrations of 4.52 mmol/L and were free from chylomicrons and hyperlipidaemia. Insulin (Alpco Diagnostics Salem, USA: catalogue number: 80-INSHU-E01.1), vascular endothelial growth factor (VEGF: Invitrogen, Life Technologies, USA: catalogue number: BMS277-2), asymmetric dimythylarginine (ADMA) and L-arginine (Diagnostika GMBH, Hamburg, Germany: catalogue number: EA207/192) concentrations were established using commercially available ELISA kits. CRP was determined by commercially available immunoturbidimetric assay (Rx Monza, Randox laboratories, UK: catalogue number: CP3885). Insulin resistance was calculated from the standard formula: (fasting glucose × fasting insulin)/22.5. A small subsample (n=6 from both groups) was used to establish changes in ADMA and L-arginine for hypothesis generating purposes.

Muscular strength and resistance exercise protocol
Muscular strength assessments were performed by AK in the HPLUWS. All training sessions were performed in local authority fitness centres under the supervision of AK. Measurement of muscular strength, prescribed training protocol, and associated data has been previously reported (Knox et al., 2017). Briefly, lower and upper body muscular strength was assessed by the 3 repetition maximum protocol utilizing the squat and bench press exercise, respectively. The protocol utilised 5 main compound exercises including back squats, bench press, deadlifts, shoulder press, and lateral pull down and progressed in a linear approach three times per week. Each exercise was prescribed at 3 sets of 10 repetitions with 2 minutes' rest between sets. The training programme was separated into two sessions; Session A and Session B, with each being performed consecutively throughout the duration of the study. Training days were separated by at least one day but no longer than two days.

Statistical analysis
Data were analysed using SPSS version 24.0 for Windows. A power calculation was performed using G*Power V3 with reference to previously published data regarding squat strength in healthy, untrained, young men (MacDonald et al., 2012). A singletailed within-group comparison revealed a required sample size of 15 per group (alpha set to 0.05 and power at 0.95). Therefore, the presented data should be considered as hypothesis generating. Group and time interactions, main effects of time and simple main effects of time were determined by a mixed model ANOVA with repeated measures and Bonferonni correction. Distribution of data was confirmed by Shapiro-Wilks' test. Homogeneity of variances were assessed using Levenes' test for homogeneity of variance. Homogeneity of covariance's was established by Box's test of equality covariance matrices. Assumptions were unviolated (p>0.05) unless otherwise stated. When Mauchlys' test of Spericity were violated (p<0.05), a Greenhouse-Geisser estimate was reported. ANOVA are presented as F test [(degrees of freedom, error terms degrees of freedom) F value, p value, partial eta-squared (pɳ 2 )]. Post-hoc data are presented as differences in mean (M), 95% CI, p value. A chi-squared test for association was conducted between groups and impaired fasting glucose levels at PRE and POST. Data is presented as group mean + standard deviation (SD). An alpha of p<0.05 was used to indicate statistical significance.

Participants
Thirty-eight males (n=19 CAUCs, n=19 SAs) were screened and deemed eligible for participation and consequently enrolled. All participants completed PRE measures and initiated supervised RES. Eight participants did not perform POST measures due to non-compliance of the protocol (n=4 CAUCs, n=4 SAs) and two had to withdraw due to personal circumstances (n=2 SAs). The final analysis consisted of 15 CAUC (25.5 ± 4.8 years) and 13 SA (25.4 ± 7.0 years) participants. Because 2 SAs participants withdrew from the study due to personal reasons (bereavement), we elected not to conduct an intention to treat analysis (See Figure 1).

CAUC
No main effects of group were seen in any biochemical marker (p > 0.05 for all). A trend was observed for a main effect of group on IR (f (1, 15) = 3.91, p = 0.067, pɳ 2 = 0.207) and CRP (f (1, 15) = 3.22, p = 0.093, pɳ 2 = 0.177). Both groups were similar at PRE for    Figure 4). Figure 5 illustrates the percentage change of biomarkers in both groups.

Discussion
The main findings of the current study demonstrate comparable responses of biomarkers of cardio-metabolic health between CAUC and SA males in response to short-term progressive RES. These responses were evident despite significant differences in muscular strength adaptation. Both groups significantly improved lipid profiles, fasting glucose and VEGF following RES. Although, no difference was observed in fasting TRIGS, insulin, L-ARG, ADMA, or IR following RES in any group, with no apparent discrepancy between groups. CRP increased in the SA group following RES with no change evident in CAUCs.
Previous findings have reported that increasing physical activity levels is beneficial for cardio-metabolic risk in SAs (Dhawan & Bray, 1997;Eriksen et al., 2015;Mathews et al., 2007) (Dhawan & Bray, 1997Eriksen et al., 2015;Mathews et al., 2007). Dhawan & Bray (1997) showed marked differences in physical activity levels between CAUCs and SAs, however increased physical activity was associated with reductions in insulin, BMI, TRIGS, and blood pressure in both groups. Similarly, Mathews et al. (2007) reported improvements in body composition, blood pressure, and lipid profiles following the Khush Dil initiative which involved participation in physical activity and nutritional workshops. Data from the SABRE study reported positive associations between physical activity and markers of cardio-metabolic health in SAs (Eriksen et al., 2015). However, conflicting data suggest a blunted response to physical activity in the SAs cohort.
Data from the Indian Diabetes Prevention Programme (Ramachandran et al., 2006) show no change in body mass following diet and physical activity advice in native Indians, although the intervention group did have a significantly lower incidence of diabetes development at year 3 than the controls. The lack of change in physiological markers are in agreement with later data that show no positive associations with increasing levels of physical activity and body composition in SAs (Yates et al., 2010). These data are supported by a more recent intervention which resulted in mixed body composition responses in SAs (Bhopal et al., 2014).
These studies not only have a key limitation of self-reported measures of physical activity, but also provide difficulty in establishing a dose-response relationship between racial groups to an exercise stimulus. The current study, to our knowledge, is the first to demonstrate a comparable cardio-metabolic response to RES between CAUC and SA males. These data provide evidence that RES is an effective method to improve cardio-metabolic health in the high-risk SA population, with no apparent discrepancy in response when compared with CAUCs. Considering previous investigations have reported a blunted response to aerobic exercise in SAs, the current data provides intriguing evidence that RES interventions may prove superior to aerobic exercise to improve cardio-metabolic health in young SAs. Further research is warranted to determine this theory.
The limited exercise response suggested by the results from previous associations of physical activity and cardio-metabolic health in SAs has initiated several comparative studies. Hall et al. (2010) demonstrated that SAs have a reduced capacity to oxidise fat during submaximal aerobic exercise in comparison with age and body composition matched CAUCs. These data suggest the SAs may need to participate in longer durations or higher exercise intensities to elicit the same physiological benefit from exercise. More recent evidence showing a blunted response in SAs from the same research group used ageadjusted regression models to determine the association between moderate intensity physical activity and cardio-metabolic risk (Iliodromiti et al., 2016). This study reports that middle-aged SA men and women need to participate in 232 min/week -1 to elicit comparable cardio-metabolic benefits as CAUCs who perform the currently recommended 150 min/week -1 . Taken these data together, they suggest a blunted response to exercise in the SA cohort. However, the current investigation demonstrates a comparable cardio-metabolic response to RES between young CAUC and SA males. Further study is warranted to establish if the ability to utilise metabolic substrates during RES is varied between racial groups. The lack of RES studies within the SA population provides difficulty to establish a comparison of existing data which potentially can contribute to our understanding of optimal RES prescription. Nevertheless, the current study demonstrates that progressive RES is effective at improving cardio-metabolic health in young CAUCs and SAs. These data also demonstrate the importance of targeting younger SA individuals to reduce the risk of developing cardio-metabolic diseases that older generations have suffered.
CRP has been regarded as an emerging risk factor for the development of cardiovascular diseases that involve systemic inflammation (Singh et al., 2008). It has been reported that SA males have a 17% higher concentration of CRP than matched CAUC males, with differences seen in individuals as young as 10 years old (Chambers et al., 2001;Cook et al., 2000). The current literature concerning the effect of RES on circulating CRP levels is mixed. RES has been shown to reduce CRP levels in African American males with no difference observed in White Americans (Heffernan et al., 2009). These results may have been a consequence of differential baseline values as the African American group presented higher levels of CRP. Additional work has also reported no change in CRP levels following 10 weeks of RES in individuals with metabolic risk factors (Levinger et al., 2009). The current study is in agreement with previous research as no changes were seen in the CAUC group. The lack of change in CRP levels following RES within the CAUC group may have been a consequence of no statistical change in body composition as there is a correlation between CRP levels and BMI (Vlachopoulos et al., 2015). However, a significant increase was seen in the SA group. This may suggest that adequate recovery from a training bout may take longer in the SA group. Previous studies have reported abnormal heart rate recovery from a graded exercise test to be positively associated with CRP levels, independent of physical fitness and disease status (Jae et al., 2007). Although the current data cannot provide evidence on recovery rates, an abnormal recovery from an exercise bout may be present in the SA population. This requires further exploration. This study does not come without several limitations. The small sample size of both racial groups may not provide sufficient statistical power to determine differences in the biomarkers that lacked disparity between groups in response to RES. Nevertheless, the current data would suggest that the biomarkers associated with cardio-metabolic health would respond similarly between CAUCs and SAs in response to RES. The small sample size also makes these data difficult to generalise to whole populations. Additionally, as previous literature using self-reported physical activity levels has suggested a blunted response to exercise in the SA population, a larger sample size that includes participants of similar age to existing literature would be necessary for validation. A larger profile of biomarkers would have also been necessary. There is strong evidence of emerging risk factors such as plasminogen activator inhibitor-1, lipoprotein A, homocysteine, tumor necrosis factor A and several adhesion molecules that are all linked with the development of cardiovascular disease. Interestingly, these compounds seem to be more prevalent in SA individuals when compared to CAUCs (Gupta et al., 2006). Inclusion of these measures would have given greater detail in the efficacy of RES in the prevention of cardiovascular risk between CAUCs and SAs. Future research concerning the SA population and RES should consider these emerging risk factors to develop the limited knowledge of exercise response within this population.
In conclusion, supervised progressive RES is effective at improving biomarkers of cardio-metabolic health within young CAUC and SA males, with no apparent discrepancy in response to exercise training. SAs significantly increased CRP levels which may indicate a requirement of a longer recovery following exercise. However, this theory has yet to be confirmed. These data provide an evidence base for interventions to target the younger generation of SAs which may assist in the reduction of future cardio-metabolic diseases that has been prevalent in the SA population for decades.

Grant information
The author(s) declared that no grants were involved in supporting this work.

Is the study design appropriate and is the work technically sound? Yes
Are sufficient details of methods and analysis provided to allow replication by others? Partly If applicable, is the statistical analysis and its interpretation appropriate? I cannot comment. A qualified statistician is required.
Are all the source data underlying the results available to ensure full reproducibility? Yes

Are the conclusions drawn adequately supported by the results? Yes
No competing interests were disclosed.

Competing Interests:
I have read this submission. I believe that I have an appropriate level of expertise to confirm that it is of an acceptable scientific standard. In the paper entitled "Caucasian and south Asian men show equivalent improvements in surrogate biomarkers of cardiovascular and metabolic health following 6-weeks of supervised resistance training" you had the aim to investigate the cardio-metabolic response to resistance exercise training in South Asian and Caucasian population. The work is well written, and presents very interesting data, however a have a few issues to clarify.

Major
The whole introduction is based on the fact that SA's are cardio-metabolic different from CAUCC's, so why you hypothesized no differences would be found in the results?
Why only male participants were enrolled in the study? You would have more complete results and an avenue to discuss if women were tested.
Data of muscular strength gain could be more clear in a table or graph.
It is not mandatory, however I feel that the authors could explore more about cardiovascular data and their relationship with the metabolic data. For example, changing in rest and recovery heart rate and heart rate variability with the RES training.