Growth , production and feed conversion performance of the gurami sago ( Osphronemus goramy Lacepède , 1801 ) strain in different aquaculture systems

Background: Giant gourami ( Osphronemus goramy, Osphronemidae), belonging to gurami sago strain, is an important economic fish species that was newly released for domestication in 2018 in Indonesia. The present study aimed to determine the growth, production and feed conversion efficiency of gurami sago strain in different aquaculture systems. Methods: A mean of 240 juveniles were stocked (initial weight mean, 54.53 g and length 13.88 cm) into concrete ponds, floating net cages and earthen freshwater ponds (12 m3) with three replicates of each. The juveniles were fed a floating commercial pellet diet containing 30% crude protein and 5% crude lipids. Feed was supplied at 3% of fish biomass per day throughout the 90 days of the experiment. The research was conducted in the area surrounding Lake Maninjau of Indonesia. Results: After 90 days, the mean weight of fish reared in concrete ponds was 166.86 g, floating net cages was 179.51 g and earthen freshwater ponds was 149.89 g. The mean final biomass was 37.64 kg for concrete ponds, 41.27 kg for floating net cages, and 33.72 kg for earthen freshwater ponds. The specific growth rates (%/day) for concrete ponds, floating net cages and earthen freshwater ponds were 0.67, 0.75 and 0.62, respectively. The feed conversion rates were 1.45 for concrete ponds, 1.30 for floating net cages and 1.87 for earthen freshwater ponds. The net yields (kg mˉ3) were 2.05 for concrete ponds, 2.27 for floating net cages, and 1.73 for earthen Open Peer Review


Introduction
Aquaculture activities have been responsible for the supply of fish for human consumption. To meet the demand for food from aquaculture production arises competition use natural resources, such as land and water [1][2][3] , included species, and aquaculture system 4-6 .
The giant gourami Osphronemus goramy Lacepède (1801) is one of the main freshwater commodities of economic importance. This species has been produced in small-scale farms for decades in Indonesia 7,8 . However, only contributed as much 6.96% of the total freshwater aquaculture production. Meanwhile, Nile tilapia, Clarias catfish, Pangasius catfish, and common carp has been contributed 37.93%, 33.35%, 12.38%, and 9.28% of 3,374,924 metric tons freshwater fish production 9 . Therefore, there are still important gaps of knowledge in its aquaculture of giant gourami.
Although the contribution from giant gourami was lowest (6.96%), but the local gurami tambago and gurami galunggung strain have been cultured in semi-intensive 7,8 . The giant gourami belongs to the local gurami sago strain has never been cultured intensively. This species is the result of newly released domestication in 2018 10 , which still limited in West Sumatera Province of Indonesia 11 . Gurami sago is an herbivorous species which can consume a variety of plants such as sente leaves (Alocasia macrorrhiza), kale (Brassica oleracea), cassava leaves (Manihot esculenta), and others young land plants. In addition, this species can eat commercial pellets, and tolerate crowded aquaculture production systems, such as earthen freshwater ponds and artificial ponds lined with membranes 8,11,12 .
The gurami sago strain has been detected as candidate species for production in middle-scale farm in Indonesia 11 . This species grows well in nursery ponds and reach a market size of 200 to 300 g per fish and a size of 50 to 100 g per fish as ornamental fishes. This characteristic creates commercial interest as a new species in an effort to develop freshwater fish farming in the future. Concrete ponds and floating net cages were options in the development of gurami sago culture. Many studies have found that continuous water flow systems in concrete ponds, artificial ponds lined with membranes, tanks, canvas tanks, pens and many other systems could be an alternative for fish aquaculture because these systems provide a high degree of control that can allow for high production 5,12-16 .
In the last decade, cage systems have received more attention from both researchers and producers. Fish farming in cages can be practiced intensively 17,18 . High production can be achieved at a low cost 19,20 . Fish farming in cages can achieve maximum growth with a high survival rate 18,21,22 . However, cage fish farming has advantages and disadvantages that must be considered before choosing a production system. The main disadvantages of fish farming in the floating net cages of lakes are that they are not ideal for land use and may cause massive fish deaths 23,24 . Meanwhile, the advantages of floating net cage aquaculture include high water circulation, solid waste not accumulating near cages, low water quality variation, and no electrical power required for water aeration 18,21,22,25,26 .
Fish production systems in many countries use a variety of methods, e.g., carp in earthen freshwater ponds 27 , giant gourami in earthen freshwater ponds and artificial ponds lined with membranes 7,12 . Nile tilapia in the ponds and cages 28 , and golden pompano in the floating cages 29 . Because the rearing of the gurami sago strain is relatively new, there are no parameters or best methods available to predict the growth performance, survival and feed conversion efficiency in a commercial rearing system. Therefore, knowledge about the contribution of gurami sago to each aquaculture system is very important to analyze. The current study was conducted to assess the growth performance, production, economic food conversion rate and waste load of feed of gurami sago strains in different aquaculture systems namely, concrete ponds, floating net cages and earthen freshwater ponds.

Ethical considerations
There are no required permits from the government of the Republic of Indonesia to culture the gurami sago (O.goramy) strain in this study in concrete ponds, floating net cages and earthen freshwater ponds in the area surrounding Lake Maninjau of West Sumatera Province of Indonesia. The study was founded by LPPM (Research and Community Service) University of Bung Hatta under the Indonesia Endowment Fund for Education, Ministry of Finance, Republic of Indonesia, through the competitive grants scheme called the Productive Innovative Research (Policy/Governance) 2019 with the contract number PRJ-99/LPDP/2019. This grant included ethical approval and permits to collect fish samples including permission to rear this species. The animals used in this study did not suffer during the experiment. Gurami sago was transported to concrete ponds, floating net cages and earthen freshwater ponds for rearing for 90 days, fed commercial pellets and measured for growth performance every 30 days. At the end of the experiment, the gurami sago were still in good condition.

Study area
The study was conducted at the Research Center of Faculty of Fisheries and Marine Science, Bung Hatta University located in the area of Lake Maninjau, Koto Malintang village, Tanjung Raya sub-district, District Agam of West Sumatera Province, Indonesia. The geographical coordinates were S:00°12'26.63"-S:00°25'02.80" and E:100°07'43.74"-E:100° 16'22.48" and the altitude was 461 m above sea level. At the location, concrete ponds, earthen freshwater ponds and floating net cages were available.

Experimental design
Each concrete pond has a size of 4×2 m, a depth of 1.5 m and a volume of 12 m 3 . It has 50 mm of middle drainage, which is covered with a net of 0.5 cm mesh to prevent juveniles from escaping and predators from entering. The water was pumped from borehole wells at a velocity of 5 litres per minute.
Each floating net cage has a size 4 × 2 m, a depth of 1.5 m and a volume of 12 m 3 , and these cages were built from resistant PVC plastic. Each cage was constructed using a monofilament net with 10 mm mesh. The floating net cages were set up in Lake Maninjau near the fish farm (maximum depth of 9 m and an average water current of 25 cm per sec). The surface of the floating net cages was covered with nets stretched (25 mm mesh) to avoid bird predators.
Each earthen freshwater pond has a size of 4 × 2 m, a depth of 1.5 m and a volume of 12 m 3 . It had 50 mm of central drainage and was covered with a net of 0.5 cm mesh to prevent fish jumping and predator entry during the rearing activity. The water was pumped from wells at a velocity of 5 litres per minute.

Sampling design
The experiment ran for 90 days beginning on 01 April and ending on 29 June 2019. Approximately 3,000 gurami sago juveniles weighing approximately 50 g were obtained from a hatchery in the Luhak sub-district in the district of Lima Puluh Kota. Fish were acclimatized with 1000 juveniles per each pond (concrete pond, floating net cages and earthen freshwater pond). Fish were acclimatized to the floating net cages (5 × 5 × 3 m) for one month prior to the experiment. In the initial growth phase, three concrete ponds, three floating net cages and three earthen freshwater ponds of 12 m 3 (three replicates) were stocked with 240 juveniles each, with a density of approximately 20 fish/m 3 . The average initial weights and lengths of juveniles were 54.51±0.45 g and 13.81±0.02 cm (mean ± SD), respectively. The length was measured using a ruler with an accuracy level of 0.1 cm. The weight of each individual was measured with an electronic balance (OHAUS, Model CT 1200-S, USA).
Fish were fed twice daily (09:00 AM and 17:00 PM) with commercial floating pellet feed (JapfaComfeed Indonesia Ltd; 30% crude protein, 5% crude lipids, 6% crude ash and 13% crude fibre) 18 . The amount of feed provided was as much as 3% per day based on fish biomass during the experiment. Every 30 days, samples were taken from ponds to monitor fish growth and to adjust the feed amount. Twenty-four fish samples were obtained from each concrete pond, floating net cage and earthen freshwater pond. 10% of the fish were sampled every month for each aquaculture system, due to giant gourami is sensitive to handling. Fish were captured at 07.00 AM with gillnets, which have a net bag with a suitable mesh size. Then, fish were anaesthetized orally with tricaine methanesulfonate (MS-222, ethyl 4-aminobenzoate methanesulfonate 98%, Sigma Aldrich Co, USA, MO; 50 mg Lˉ1), based on the dosage used for Hemibagrus wyckii 30 .

Water quality
Water parameters were recorded weekly in the concrete ponds, floating net cages and earthen freshwater ponds. The water temperature (ºC) and dissolved oxygen (DO; mg Lˉ1) were measured with an oxygen metre (YSI model 85). The pH values were determined using a pH metre (Digital Mini-pH Metre, 0-14PH, IQ Scientific, Chemo-science (Thailand) Co., Ltd, Thailand). The levels of ammonia (NH 3 ; mg.Lˉ1), nitrite-nitrogen (NO 2 -N; mg Lˉ1), nitrate-nitrogen (NO 3 -N; mg Lˉ1), chemical oxygen demand (COD; mg Lˉ1), biological oxygen demand (BOD 5 ; mg Lˉ1), alkalinity (mg Lˉ1), hardness (mg Lˉ1), total dissolved solids (TDS; mg Lˉ1) and total suspended solids (TSS; mg Lˉ1) were measured in each aquaculture system with replication according to standard procedures 31 . The nets of the floating cages were cleaned routinely to maintain water circulation in the fish rearing areas. The walls of the floating net cages were cleaned by divers in the water.

Measurement parameters
The gurami sago were reared for 90 days, and the survival rate was estimated by checking the aquaculture systems every day and recording the results. Dead fish were removed immediately. The survival rate percentage was calculated by subtracting the number of dead fish from the initial number of the stock. The parameters were analyzed according to Aryani et al. 8  For each aquaculture system, the final total length (cm) and final total weight (g) were used to determine the relationship of W = aL b , where W is the total wet weight (g), L is the total length (cm) and a and b are variables of the length-weight relationships (LWRs) equations. These variables were estimated by the least square regression method. A t-test was used for comparison of the b values obtained in the linear regressions with the isometric value by equation 33 : t s = (b -3)/S b , where t s is the t-test value, b is the slope and S b is the standard error of the slope (b). The comparison of the obtained values of the t-test with the respective table critical values allowed for the determination of whether the b values were statistically significant as well as their inclusion in the isometric range (b=3) or allometric range (negative allometric; b<3 or positive allometric; b>3). The degree of correlation between the variables was computed to determine the coefficient, R 2 . Fulton's condition index was calculated as K=100(W/L 3 ) 33 , where K = Fulton's condition index, W = weight, and L= length.

Data analysis
The data were analyzed using SPSS software (version 16.0 for Windows; SPSS Inc., Chicago, IL). Kolmogorov-Smirnov statistics were used to test data normality. Then, Levine's test was used to analyse the absolute residuals from homogeneity. One-way ANOVA was used to analyze the effect of each treatment, followed by post hoc Duncan's multiple range tests 34 . The 95% confidence level (p<0.05) was considered as the threshold to identify significant differences. All means are given with ± standard deviation (±SD). The canonical discriminant functions were used to analyze the water quality grouping between rearing systems.

Results
The overall survival rate of fish in different aquaculture systems was greater than 89.44%. The culture system had a significant effect (p<0.05) on the mean final body weight (g), final biomass (kg), weight gain (g), gross yield (kg mˉ3), net yield (kg mˉ3), absolute growth rate (g dayˉ1), specific growth rate (% dayˉ1), AFCR, and economic food conversion rate (US$/kg gain) after 90 days of culture (Table 1). In contrast, the culture system did not significantly (p>0.05) affect the mean final total length, feed intake (kg) or Fulton's K. The economic AFCRs were US$1.45 for concrete ponds, US$1.30 for floating net cages and US$1.87 for earthen freshwater ponds.
During the 90 days of the experiment, the gurami sago reared in floating net cages grew faster than those reared in concrete ponds and earthen freshwater ponds ( Figure 1). At the end of the experiment, the fish reared in the floating net cages had a larger size distribution than that of the fish reared in the concrete ponds and earthen freshwater ponds throughout the 90 day trial ( Figure 2). The mean final body weights of the gurami sago reared in concrete ponds, floating net cages and earthen freshwater ponds were 166.86 g, 179.51 g, and 149.89 g, respectively. The net yield was 2.05 kg mˉ3 for concrete ponds, 2.27 kg mˉ3 for floating net cages and 1.73 kg mˉ3 for earthen freshwater ponds during the 90 days of rearing. The FCE and waste load at 90 days of culture were significantly (p<0.05) affected by the different rearing systems. A summary of the FCR, FCE and waste load feed from the five aquaculture species is presented in Table 2.
The growth rates of gurami sago based on body weight were described according to the following exponential equation: W = 60.875e 0.0498t (with R 2 = 0.83) for the concrete pond, W = 48.580e 0.0613t (with R 2 = 0.75) for the floating net cage and W = 55.7050e 0.0623t (with R 2 = 0.75) for the earthen freshwater pond. The length-weight relationships for the gurami sago reared in concrete ponds were shown by W = 7.9368L 1.0146 (with R 2 = 0.83, Figure 3) and by W = 3.7760L 1.2641 (with R 2 = 0.75, Figure 4) for the floating net cages and by W = 9.3106L 1.0056 (with R 2 = 0.75, Figure 5) for the earthen freshwater ponds. The three b-values of each aquaculture system differed from 3.0 (b<3, p<0.05) indicating negative allometric growth. The Fulton's condition index in the concrete pond, floating net cages and earthen freshwater pond were 2.45, 1.91, and 3.36, respectively.
In this study, the water quality was recorded weekly from each aquaculture system during the experiment period and showed significant differences (p<0.05) in terms of TDS, TSS, DO, COD,      BOD, ammonia, nitrites, nitrates, pH, alkalinity and hardness, only water temperature did not show a significant difference. Furthermore, in the principal component analysis, PC1 accounted for 66.67% of the 12 parameters of water quality, which had a positive correlation with all water quality parameters. This result shows that value has an effect on the water quality parameters in aquaculture systems. Alkalinity, hardness, pH, and dissolved oxygen make high contributions to the aquaculture system (Table 3). The plot of PC1 and PC2 shows highly isolated water quality parameters between concrete ponds, floating net cages and earthen freshwater ponds ( Figure 6).

Discussion
The aquaculture industry needs environmentally friendly aquatic ecosystems. Therefore, aquaculture practices must use aquaculture systems that minimize waste loads and increase added value 1,3,38,39 . In fact, the diversification of aquaculture systems with the efficient use of land resources can increase aquaculture production 28,40 . The comparisons between concrete ponds,  floating net cages and earthen freshwater ponds are relevant to determine their relative per unit volume performance of juveniles-rearing of gurami sago. The rearing of gurami sago is an alternative diversity of aquaculture that can contribute to the development of commercial production in the future.
Gurami sago was successfully reared in concrete ponds, floating net cages and earthen freshwater ponds. However, their growth performance was best in the floating net cages. The high survival rate of gurami sago was found in the floating net cages, which was similar to the gurami tambago strain 8 and gurami sago in the artificial ponds lined with membranes 12 . On the other hand, the survival rates of gurami sago in earthen freshwater ponds (89.44%) were higher than those of carps (65.74%) and stinging catfish (69.00%) in freshwater ponds 27 .
The growth rate of gurami sago, with an average initial weight of 54.18 g, was faster in floating net cages than in concrete ponds and earthen freshwater ponds, with specific growth rate (SGR, % day -1 ) values of 0.67, 0.75 and 0.62, respectively. In contrast, Budi et al. 41 stated that giant gourami belonging to the local gurami soang strain in the laboratory with initial weight of 15.83 g had faster growth with an SGR value of 2.13% dayˉ1. The specific growth rate of fish seems to be influenced by the initial weight, strains and aquaculture systems. The economic AFCR value of fish fed in floating net cages was lower than that of fish fed in concrete ponds and earthen ponds. Therefore, it can reduce the cost of feed and increase the economic benefits to producers. This condition indicates that the culture of gurami sago in floating net cages gives fish a chance to consume more feed. However, this AFCR was lower than that of Nile tilapia 42,43 , and giant gourami 8 , and higher than the African catfish AFCR value 44 .
In this study, the growth performance of different gurami sago individuals in each aquaculture system was caused by differences in water quality. The PCA shows that there are differences in water quality among concrete ponds, floating net cages and earthen freshwater ponds. The alkalinity, hardness, and pH might affect the growth performance of gurami sago in aquaculture systems. Pouil et al. 7 state that nutrient input in the cultured of giant gourami in the earthen freshwater ponds strong correlation with sediment nutrient accumulation, of which 61% total nitrogen and 77% phosphorus inputs were trapped in the accumulated sediments, which directly impacts to aquatic environment. Furthermore, Boyd et al. 45 stated that the productivity of aquatic ecosystems and aquaculture production can be influenced by water quality, such as alkalinity, hardness and pH. Many studies have found that growth performance can be affected by water temperature 46,47 , DO level 48 and nitrite-nitrogen 27 .
The aquaculture system influences the production of gurami sago. The highest production was found in the floating net cages, with a value of 3.36 kg mˉ3. However, its production was lower than that of other freshwater cages, for example 4.  36 . It seems that different levels of aquaculture production can be influenced by species diversity, stocking density and duration of aquaculture. Giant gourami can produce a maximum profit after 324 days of aquaculture 51 .
Herein, we recommend gurami sago strain aquaculture in concrete ponds, floating net cages and earthen freshwater ponds for 324 days. According to De Oliveira Continho et al, 52 fish reared in cages can increase the variation in weight production. In contrast, the freshwater cages have been marred by increasing the frequencies of fish mortality, causing negative implications to finances and the environment 23,24,53 . Bosma and Verdegem 54 reported that the direct risks related to aquaculture in ponds were habitat destruction, suboptimal freshwater consumption, organic pollution, eutrophication, and water contamination with pesticides. These factors can cause production to decline and cause low economic value.
In this study, after the analysis of growth performance and production, we also analyzed the length-weight relationship and condition factor (K) from aquaculture systems.

Conclusion
In conclusion, our study showed that gurami sago strain can be efficiently reared in concrete ponds, earthen freshwater ponds and floating net cages. For all tested parameters, the best aquaculture system was found in the floating net cages. Nevertheless, further investigations on fish farming in the floating net cages which a technically feasible and economics at a larger scale are needed to determine commercial interest and environment impacts, especially on water quality, in an effort to develop of gurami sago fish farming in Indonesia. This project contains the following underlying data: - Table 1. Sample size of weight and length of the gurami sago strain (0 days, 30 days, 60 days and 90 days) in the concrete pond culture (N=24) - Table 2. Sample size of weight and length of the gurami sago strain (0 days, 30 days, 60 days and 90 days) in the floating net cage culture (N=24) - Table 3. Sample size of weight and length of the gurami sago strain (0 days, 30 days, 60 days and 90 days) in the earthen freshwater pond culture (N=24) - Table 4. Sample size means of initial weight, final body weight and weight gain of gurami sago (N=24) - Table 5. Sample size means of initial length, final total length and length increase of gurami sago (N=24) - Table 6. Data on mean initial biomass, final biomass and gross yield of gurami sago (N=24) - Table 7. Data on mean SGR, feed intake and apparent feed conversion rate of gurami sago (N=24)

Data availability
- Table 8. Data on mean economic food conversion, feed conversion efficiency and waste load of feed (N=24) - Table 9. Data on mean growth (g) of gurami sago at 0 days, 30 days, 60 days, and 90 days (N=24) - Table 10. Data on mean size distribution (g) of gurami sago in the different aquaculture systems in the 90-day trial (N=72).
- Table 11. Row data for water quality parameters of reared gurami sago in different aquaculture systems for each month.

RESULTS AND DISCUSSION
Well written and organized.

CONCLUSION
As mentioned in the summary: "our study showed that gurami sago strain can be efficiently reared in floating net cage", in which term gurami can be efficient rear in floating net cage for all parameters or some? Please add information more briefly.

If applicable, is the statistical analysis and its interpretation appropriate? Yes
Are all the source data underlying the results available to ensure full reproducibility? Yes

Are the conclusions drawn adequately supported by the results? Yes
Yes

If applicable, is the statistical analysis and its interpretation appropriate? Yes
Are all the source data underlying the results available to ensure full reproducibility? Yes

Are the conclusions drawn adequately supported by the results? Yes
Indonesia showing that this country is among the main producing country in the world. Some sentences could be added regarding the important role of freshwater aquaculture in the country by providing food and economic resources as well as employment in rural communities… Then the second paragraph should be focused on giant gourami as Authors did but maybe in a awkward way because the sentences said that giant gourami is important but this species accounts for only 7% of the national production. Even if it is true, giant gourami production represents now more than 100,000 tonnes/year of production and this production is growing (latest available production volumes and increasing rate per year should be provided). This species has high patrimonial and economic values (among the most expensive freshwater fish species in Indonesia). Instead of having the comparison of production volumes with other freshwater fish species, I suggest to the Authors to provide data and relevant arguments to highlight the importance of giant gourami in Indonesia. Most of the information should be available in the following references: FAO (2019)  Thus, the structure of the Introduction could be, with appropriate transition sentences: 1/ Importance of freshwater finfish aquaculture in Indonesia 2/ Giant gourami among the main freshwater commodities in the country 3/ Gaps of knowledge in gourami aquaculture 4/ Rearing structures for giant gourami aquaculture 5/ Objectives of the study In my previous report I highlighted the confusion between "strain" and "species" with those terms used interchangeably throughout the manuscript. Again, gurami sago is not a new species as the Authors said, it is a strain of giant gourami Osphronemy goramy. Here again, as I said on my previous report, I do not see the point to be focused on this strain in the Introduction because the findings from this study may be interesting for giant gourami in general. The strain used should be only mentioned in the Materials and Methods section.

Materials and Methods:
In the subsection "Ethical considerations", I think the sentence "The animals used in this study did not suffer during the experiment" should be deleted because it is always difficult to assess suffering in fish. The most important information regarding ethical considerations was already provided.
As said in the previous review performed by Reviewer 2, water parameter values should be included in a Table within the manuscript, not as additional data.
Change "Sampling design" by "Experimental procedures" In this subsection, when the origin of the fish is provided I suggest saying that the fish belonged to the "Gurame sago" strain, moving the information regarding this strain from the Introduction.
I suggest rephrasing the following sentence "Twenty-four fish samples were obtained from each concrete pond, floating net cage and earthen freshwater pond. 10% of the fish were sampled every month for each aquaculture system, due to giant gourami is sensitive to handling." to "Twenty-four fish were sampled monthly from each concrete pond, floating net cage and earthen freshwater pond. The sample size and sampling frequency were selected in order to sample 10% of the total number of fish for each rearing structure while limited stress for the fish caused by handling." I am not sure to understand how the "Waste load of feed" and "FCE" were evaluated since they require to be able to quantify the feed ingestion, right? I do not know how such an estimation can be performed in the tested rearing systems. The Authors should clarify this point already highlighted in the previous report.
For the data analysis, assumptions of ANOVA (normality and variance homoscedasticity) should be tested and met on residuals, it should be clearly indicated in the manuscript. "Levine's test" should be corrected by "Levene's test". "One-way ANOVA was used to analyze the effect of each treatment", effects on what?
The Authors should be precise regarding the data analyzed through ANOVA. I guess all of them did not meet the assumptions for using parametric ANOVA.
In the same section, the Authors said they used discriminant analysis to examine grouping of the rearing systems using water quality parameters but the terms "Principal Component Analysis (PCA)" were used in their response to the reviewer comments that are not referring to the same statistical approach. The actual analysis performed should be clarified and better described in the data analysis section by explaining potential data transformation prior to the analysis, how the principal components have been selected… Tables  As I said in my

M&M:
"Feed conversion efficiency (FCE) = [fish weight gain (g)/total feed ingested (g)] Apparent feed conversion rate (AFCR) = supplied feed/increase fish weight" -It is interesting that you have two indicators of feed performance where one is listed as apparent. I presume that feed waste was not collected in any of your rearing systems, and therefore all of your feed intake are apparent and based on "supplied feed", also the FCE.

Results:
One of the things that can explain the observed differences in growth performance is likely to be your water quality parameters. While it is fine with the PCA plot, I would really like to see the water quality measurements in a table. Once these are available, perhaps it would be possible to discuss which water quality parameters would be essential to control to successfully produce gourami in land-based systems.

Is the work clearly and accurately presented and does it cite the current literature? Yes
Is the study design appropriate and is the work technically sound? Yes

If applicable, is the statistical analysis and its interpretation appropriate? Partly
Are all the source data underlying the results available to ensure full reproducibility? Partly

Are the conclusions drawn adequately supported by the results? Partly
Competing Interests: No competing interests were disclosed.
Reviewer Expertise: Aquaculture nutrition and bioenergetics "gurami sago" in three different rearing structures: concrete ponds, floating net cages and earthen freshwater ponds. Overall, the study presented is quite simple but nevertheless interesting especially because some important gaps of knowledge remain in giant gourami aquaculture. Thus, results presented, showing better growth performance and feed conversion efficiency when giant gourami is reared in floating net cages, are relevant to the field. Having said that, I think that the present version of the manuscript may be improved in some ways.
My main concerns are related to some methodological aspects as well as the presentation of the data.
I addressed below some general comments regarding on the different sections of the manuscript. I hope the following comments help the authors in revising the manuscript.

Introduction:
The description of the rationale of the study could be improved. I suggest to go straight to the point with a first paragraph explaining why giant gourami is important in Indonesian aquaculture and avoiding too general information. Authors may provide some production figures and explain, based on relevant references, that giant gourami is an emblematic local species with high practical and market value, omnivorous with a strong vegetarian component as thus, a candidate species for improving sustainability in aquaculture.
Authors should clearly state that, although giant gourami has been reared for decades in Indonesia, there are still important gaps of knowledge in its aquaculture.
Another point that is true throughout the manuscript: be careful in the use of "strain" and "species". These two terms seem to be used as synonyms in the manuscript although they refer to different concepts. I am not sure that the focus done on the strain used is so important in the Introduction. I think that the results provided here are useful for the species itself and not only this specific strain.

Methods:
Overall, I found the Methods well-presented and informative enough. Nevertheless, I have one important concern regarding the statistics. Indeed, water parameters were recorded monthly, meaning that only 3 values per rearing structures are available to perform the canonical discriminant functions (CDF). Considering the variations of most of the measured parameters that can be occur in rearing structures such as shallow earthen ponds sometimes on the same day, I think such analysis is not appropriate.
Furthermore, Authors should state why only 10% of the fish were sampled every month. I guess is because giant gourami is sensitive to handling but this information may be interesting to add.

Results:
As I already mentioned, I have some doubts regarding the validity of the CDF using water quality data.
I believe that the presentation of the results can be improved. Authors should provide visible standard deviations values and statistical differences in the Figures when it is appropriate and better axis scale in order to improve data readability. For allometric relationships, p-values for model estimates should be provided. Table 1: "Final food conversion rate (Fulton's K)", I guess it should be changed by "Condition factor (Fulton's K)"

Discussion:
Although water quality is likely a key parameter to explain some of the observed differences in zootechnical performances among the rearing structures, unfortunately, since water parameter values were recorded only once a month, I think that there is not enough information provided to use these results.

Conclusion:
In the concluding paragraph, I expected clear recommendations for giant gourami aquaculture based on the findings from this study.