Biodesulfurization of Fossil Fuels: Analysis and Prospective

Wisam Mohammed Kareem Al-Khazaali; Seyed Ahmad Ataei; Saeed Khesareh

doi:10.12688/f1000research.133427.1

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Review

Biodesulfurization of Fossil Fuels: Analysis and Prospective

[version 1; peer review: 2 approved, 3 approved with reservations]

Wisam Mohammed Kareem Al-Khazaali ¹, Seyed Ahmad Ataei², Saeed Khesareh²

PUBLISHED 08 Sep 2023

Author details Author details

¹ Chemical Engineering Sec., Designs Dept., Projects Div., Misan Oil Company, Misan Governorate, Iraq
² Department of Chemical Engineering, Faculty of Engineering, Shahid Bahonar University of Kerman, Kerman, Iran

Wisam Mohammed Kareem Al-Khazaali
Roles: Methodology, Writing – Original Draft Preparation

Seyed Ahmad Ataei
Roles: Supervision

Saeed Khesareh
Roles: Validation

OPEN PEER REVIEW

REVIEWER STATUS

This article is included in the Energy gateway.

Abstract

Biodesulfurization (BDS) of fossil fuels is a promising method for treating the high content of sulfur in crude oils and their derivatives in the future, attributed to its environmental-friendly nature and the technical efficient ability to desulfurize the organosulfur compounds recalcitrant on other techniques. It was found that the bioreaction rate depends on the treated fluid, targeting sulfur compounds, and the microorganism applied. Also, many studies investigated the operation conditions, specificity, and biocatalysts modification to develop BDS efficiency. Furthermore, mathematical kinetics models were formulated to represent the process. In this review, the previous studies are analyzed and discussed. This review article is characterized by a clear picture of all BDS's experimental, industrial, procedural, theoretical, and hypothetical points.

Keywords

Biodesulfurization, aerobic, anaerobic, recalcitrant organosulfur compounds.

Corresponding author: Wisam Mohammed Kareem Al-Khazaali

Competing interests: Wisam Al-Khazaali is employee in Misan Oil Company, but the company did not fund this work.

Grant information: The author(s) declared that no grants were involved in supporting this work.

Copyright: © 2023 Al-Khazaali WMK et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

How to cite: Al-Khazaali WMK, Ataei SA and Khesareh S. Biodesulfurization of Fossil Fuels: Analysis and Prospective [version 1; peer review: 2 approved, 3 approved with reservations]. F1000Research 2023, 12:1116 (https://doi.org/10.12688/f1000research.133427.1) First published: 08 Sep 2023, 12:1116 (https://doi.org/10.12688/f1000research.133427.1) Latest published: 08 Sep 2023, 12:1116 (https://doi.org/10.12688/f1000research.133427.1)

1. Introduction

Fossil fuel (FF) is a crucial source of energy and power in numerous industries and aspects of daily life. However, to ensure Health, Safety, and Environmental (HSE) protection, it must adhere to relevant standards before use, including the treatment of sulfur compounds. Fossil fuels come in various forms, such as coal, crude oil, tar, petroleum fractions, shale oil, and sands. Regulations for HSE, quantity assurance, and quality control (QQHSSE) are based on specific criteria, including:

• The technical quality of crude oil is determined by its specifications, including the American Petroleum Institute (API) density, viscosity, and combustion heat value. These specifications can be influenced by the presence of organic sulfur compounds in refineries, as highlighted by various studies (Adlakha et al., 2016; Bergh, 2012; Sadare et al., 2017; Alves et al., 2015).
• The sustainable quantity of crude oil is indirectly influenced by the presence of sulfur, which can limit or reduce its marketability due to its negative impact on QQHSSE criteria. As a result, many efforts are being made towards clean energy, which has led to a commitment to meeting quality standards in various regions.
• The health impact of sulfur is significant, as sulfur dioxide emissions can lead to safety hazards, corrosion, and environmental leaks that can cause heart disease, asthma, and respiratory ailments, as evidenced by various studies (Sadare et al., 2017; Srivastava, 2012). Hydrogen sulfide (H₂S) is another hazardous sulfur compound that can cause acute toxicity, leading to fatalities in natural settings and workplaces. Exposure to H₂S can also result in loss of consciousness, paralysis, nervous system disorders, cardiovascular disorders, ocular disorders, gastrointestinal disorders, and even death, as highlighted by Rashidi et al. (2015).
• The safety implications of sulfur compounds are significant, as their release into the environment can lead to pollution, acid rain, and damage to buildings and forests, as noted by Sadare et al. (2017). Additionally, sulfur compounds can poison the catalytic converters in automotive engines, leading to premature failure and reduced efficiency, as highlighted by Sadare et al. (2017) and Bergh (2012). The presence of sulfur in liquid fuels can also harm vehicles and lower the efficiency of catalytic converters, as noted by Sadare et al. (2017) and Srivastava (2012). Furthermore, sulfur can cause corrosion of pipelines, pumps, fittings, and refining equipment, as noted by Adlakha et al. (2016). The poisoning or deactivation of catalysts used in refining is another concern, as higher levels of sulfur in petroleum distillates can lead to the deactivation of catalysts by poisoning the fluid catalytic cracking (FCC) and hydrocracking processes that convert heavy distillates to lighter ones, as highlighted by Sadare et al. (2017).
• Environment: The combustion of fossil fuels can result in the release of harmful components such as SO_x, NO_x, CO₂, and H₂S, which can have serious environmental consequences. These emissions can cause acid rain, acid deposition, and severe air pollution, which can be detrimental to agriculture, human health, and wildlife (Chen et al., 2019a; Sadare et al., 2017; Srivastava, 2012; Ansari, 2017; Pacheco et al., 2019).
• As a result of these concerns and in order to implement risk control measures, the Clean Air Act of 1964 was introduced, with subsequent amendments in 1990 and 1999. These laws were strengthened with even more stringent requirements aimed at further reducing the quantity of sulfur released into the air. Currently, the U.S. Environmental Protection Agency (EPA) has revised the sulfur standards to the following levels:
• The current standard of 500 ppmw for diesel fuel was replaced with a stricter requirement of 15 ppmw, which became effective in mid-2006.
• The standard for reformulated gasoline has been revised to lower the limit from 300 ppmw to 80 ppmw, with a yearly average not exceeding 10 ppm, effective from January 1, 2004.

Similarly, the EU has also implemented similar modifications by setting a sulfur limit of 10 ppmw for diesel fuels and gasoline in 2005 to address these adverse effects. In order to mitigate these effects, environmental agencies in various countries around the world have imposed more stringent legislative provisions on the total sulfur content of oil (Chen et al., 2018, 2019a, 2019b).

This review provides a more comprehensive analysis of modern chemicals and biotechnology compared to previous review papers. Additionally, the study offers a significant insight into the modeling and optimization of biodesulfurization.

2. Desulfurization methods

There exist various treatment methods that can be classified into three categories: physical, chemical, or biological. These methods include lab or industrial techniques, such as adsorptive desulfurization (ADS) (Akhmadullin et al., 2012; Al-Otaibi., 2013; Dantas et al., 2014; Mujahid et al., 2020; Sadare et al., 2017), alkylation-based desulfurization, microbial or bacterial desulfurization (BDS) (Chen et al., 2019), catalytic reaction treatment (Akhmadullin et al., 2012; Jarullah, 2012), sodium metal reaction (Chavan et al., 2012), Caustic Washing Method for demercaptanization and H₂S removal, Chlorinolysis-based desulfurization, Extraction (desulfurization by extraction, EDS) (Heidari et al., 2013; Qader et al., 2021), dry gas desulfurization, hydrodesulfurization (HDS), oxidative desulfurization (ODS) (Sadare et al., 2017), and supercritical water-based desulfurization (SWD) (Siddiqui and Ahmed, 2016).

Compared to other methods that are not yet commercialized, BDS is capable of removing refractory heterocyclic compounds in crude oil to achieve ultra-low sulfur diesel (ULSD) with high efficiency and at an ultra-low cost (Malani et al., 2021). Undoubtedly, BDS is suitable for desulfurizing heavy oils, such as shale oils, which have higher concentrations of thiophene (Mohebali and Ball, 2016; Pacheco et al., 2019), making it a promising method for commercialization. Moreover, it is more efficient and cost-effective than HDS.

Harsh reaction conditions are required for the hydrodesulfurization (HDS) process to remove certain recalcitrant sulfur-containing compounds like alkylated dibenzothiophenes (DBTs), which can lead to fuel degradation and reduced quality. In contrast, bacterial desulfurization (BDS) has shown high efficiency and low cost for ultra-low sulfur diesel (ULSD) production and is a promising alternative to HDS. Therefore, several studies have been conducted to facilitate the industrial application of BDS due to its advantages over HDS (Chen et al., 2019b; Mohebali and Ball, 2016; Sadare et al., 2017).

Some examples of combined desulfurization methods are BDS-ODS-EDS (Duissenov, 2013), EDS-HDT (Hamad et al., 2013), OEDS (oxidation extraction desulfurization) (Awad, 2015; Jiang et al., 2018; Sadare et al., 2017), and the microwave catalytic hydrogenation process (Duissenov, 2013; El-Gendy et al., 2014).

3. Susceptibility of treated oils and biodesulfurizers

BDS is capable of treating various pretreated crude oils and its derivatives, including LPG, gasoline, jet fuel, kerosene, fuel oil, and gas oil. HCS may be present in these fractions or the entire crude oil, including alkyl sulfides (thioesters), carbon bisulfide, disulfides, hydro thiophene, mercaptans (thiol “alkyl hydrosulphide” and thiophenol), thiophenes, thiophane, thiolates, sulfides (non-cyclic sulfides, cyclic sulfides), sulfoxides, sulfones, and sulfonic acid.

Few studies and patents have been conducted on the application of Aspergillus flavus, Achromobacter Spp., Leptospirillum Spp., Pseudomonas Spp., Sulfolobus Spp., Thiobacillus Spp., Rhodococcus Spp., Sphingomonas subarctica, Bacillus Spp., Desulfovibrio desulfuricans, Pyrococcus Spp., Desulfomicrobium scambium, Desulfovibrio longreachii, or Pantoea agglomerans for the desulfurization of whole crude oil under aerobic or anaerobic conditions (Adegunlola et al., 2012; Gunam et al., 2013; Yang et al., 2016). The advantage of BDS for the desulfurization of whole crude oil is that it can reduce the cost of the desulfurization treatment in refineries (El-Gendy and Nassar, 2018; Al-Jailawi et al., 2015).

In addition, microorganisms such as Mycobacterium goodii, Pseudomonas Spp., Gordonia Spp., Rhodococcus Spp., Mycobacterium phlei, Paenibacillus Spp., Rhodococcus globerulus, and Nocardia Spp. have also been used for BDS treatment of gas oil, gasoline, petro-diesel fuels, petroleum wastes, fuel oil, and cracked stocks (El-Gendy and Nassar, 2018; Li and Jiang, 2013; Murarka and Srivastava, 2020; Nassar et al., 2021a).

In addition, model compounds representing the recalcitrant HCS in fossil fuels can be used to study the efficiency of BDS. These compounds can be in the form of pure or mixture solutions such as B.T., DBT, DBTO2, M DBTSO2, MgSO4, BNT, DBS, 2,8 DMDBT, 2,6 DNDBT, DMDBT, thianthrene, and dibenzyl sulfide (Alejandro et al., 2014; Boshagh et al., 2014; Bordoloi et al., 2014; Jiang et al., 2014; Kawaguchi et al., 2012; Nassar et al., 2013; Sohrabi et al., 2012; Zhang et al., 2013). It is worth noting that microorganisms used for BDS on water or coal could also be effective for crude oil applications (Feng et al., 2018). A recent study conducted in 2022 by Al-Kazaali (Al-Kazaali and Ataei, 2022) investigated the BDS of heavy sour crude oil using various microorganisms and media. Table 1 summarizes the recent research on BDS application on various fractions of crude oil using different microorganisms.

Table 1. Last Recent treatment case studies of real and model fractions.

Fraction	Microorganism	Conditions	Response	Reference
Coal	Acidithiobacillus ferrooxidans LY01 domesticated with ferrous iron and pyrite	28°C 180 rpm 3 d	67.8% at case: pyrite 45.6% at the case: Fe (II)	(Yang et al., 2016)
DBT 0.3 mM	Paenibacillus PO-2 Basal salt medium, sulfure-free	30°C 180 rpm 3 d	95%	(Derikvand and Etemadifar, 2015)
Crude oil light Cs=1.5%	Bacillus subtilis Wb600 Incubator/shaker/thermos	T 308 K 150 rpm t 90 h	40%	(Nezammahalleh, 2015)
Crude oil, gas oil	Gordonia sp. IITR100	30°C 180 rpm 7 d	76.1% at the case: HCO 9.8% at case: gas oil-1 70% at the case: gas oil-2	(Adlakha et al., 2016)
B.T., DBT, MDBT, DMDBT, DBS	Stenotrophomonas isolates AK9 Chemical-defined medium, sulfur-free	30°C 180 rpm 4 h	90% DBT	(Ismail et al., 2016)
Crude oil, gas oil, kerosene,benzen, DBT,DBTO2,MgS4	Rhodococcus Pseudomonas Bacillus	30°C 200 rpm 15 d	30%	(Shahaby and Essam-El-din, 2017)
DBT 0.1 mM MgSO4: 0.2 mM	Ralstonia eutropha strain FMF Basal Salt medium, pH 7	30°C 150 rpm 24 hr	20%	(Dejaloud et al., 2017)
Wastewater	Halothiobacillus neapolitanus Basal Salt medium	30°C 170 rpm 100 hr	85%	(Feng et al., 2018)
DBT	Gordonia alkanivorans strain 1B			(Pacheco et al., 2019)
DBT	Gordonia sp. SC-10 Sulfur-free medium	30°C 160 rpm 5 d	81%	(Chen et al., 2019a)
Gas oil	Gordonia sp. SC-10 Sulfur-free medium	30°C 160 rpm 5 days	80% at case: OWR 1/12	(Chen et al., 2019b)
DBT	Ralstonia eutropha PTCC 1615	pH 6-9	98% at case: pH 8	(Dejaloud et al., 2019)
B.T., DBT, and derivatives	Klebsiella Pseudomonas Rhodococcus Sphingobacterium Chemical-defined medium	180 rpm	25 %	(Awadh et al., 2020)
Gas oil	Paenibacillus glucanolyticus HN4 Basal Salt medium	30°C 150 rpm	<80%	(Nassar et al., 2021a)
DBT and derivatives	Gordonia alkanivorans strain 1B BSM-SF	30°C 150 rpm 14 hr	average q2-HBP, 2-HBP specific production rate (μmol/g (DCW): 1.5	(Silva et al., 2020)
Gas oil 169 mg/l DBT, MDBT, DMDBT	Gordonia sp. SC-10 SFM	30°C 160 rpm 4 d	88%	(Chen et al., 2021)
Gas oil 280 mg/l, BT, DBT, DBDBT, DHDBT	Sphingomonas subarctica T7b MSSFCA medium was	27°C 273 rpm 5.5 d	41.4% at case: gas oil 82% at the case: DBT 80% at the case: DBDBT	(Gunam et al., 2021b)
Crude oil 0.4%	Pseudomonas aeruginosa NB	30°C	50%	(Saeed et al., 2022)
Crude oil 4%	Ralstonia eutropha Rhodococcus erythropolis Acidithiobacillus ferrooxidans Acidithiobacillus thiooxidans	30-50°C	>80%	(Al-Kazaali and Ataei, 2022)

Desulfurizing microorganisms can be classified based on their air requirement (Raheb, 2016) into the following categories:

• Aerobic conditions
• Anaerobic conditions
• Favored anaerobic

3.1 Aerobic conditions

The reaction mixture for biodesulfurization contains the fluid to be treated, which could be crude oil, one of its derivatives, or model compounds of the recalcitrant HCS such as benzothiophene (BT) or DBT. This fluid is mixed with the main nutrient medium, trace element solution, and vitamin solution. The microorganism is then added to this reaction mixture. The equipment used for BDS can be either a simple incubator or an airlift reactor.

Rhodococcus strains have been widely used in BDS to desulfurize various fractions, including pure DBT and its alkylated derivatives such as 4,6-DB-DBT and 4-HDBT, as well as light gas oil by Sphingomonas subarctica T7b (Mohamed et al., 2015). Other microorganisms that have been applied in BDS for real fluids and model compounds include Bacillus strains such as subtilis DSMZ 3256, Gordonia strains such as IITR 100 and D. alkanivorans strain 1B, Klebsiella sp. 13T, Mycobacterium goodii X7B, Pseudomonas strains such as P. delafieldii R-8, P. aeruginosa, and P. putida CECT5279, Paenibacillus validus strain PD2, and Stachybotrys sp. (AL-Faraas et al., 2015; Boshagh et al., 2014; Bhatia and Sharma, 2012; Chauhan et al., 2015; Derikvand and Etemadifar, 2015). Moreover, the BDS of TH, BTH, or DBT by sulfur-reducing bacteria (SRB) has been studied, as well as coal and pyrite by Theobacillus ferroxidans and Ascidians Spp. Brierley (Rossi, 2014).

3.2 Anaerobic conditions

Anaerobic BDS has been reported for crude oil and its distillates using microorganisms such as Desulfovibrio desulfuricans M6 and extreme thermophile Pyrococcus furiosus. In addition, BDS of thiophene, benzothiophene, and benzothiophene were detected in anaerobic mixed microbial communities of SRB from three Russian oilfields. These communities used reducing processes and produced sulfide-containing hydrogen, lactate, and ethanol as potential electron donors. The conversion of DBT to biphenyl was observed, but HC products from BTH and TH desulfurization remained undetected. Further enrichment of thiophene as the only electron acceptor led to the disappearance of conversion activity, and homo-acetogenic bacteria were abundantly present. Attempts were made to isolate sulfide-producing bacteria to attain stable conversions of THs, but the activity remained low, and homo-acetogenesis was dominant. However, the rate of anaerobic BDS is low, which prevents commercialization.

3.3 Favored anaerobic

Many aerobic microorganisms have been studied for crude oil BDS, including Pantoea agglomerans D23W3 and Klebsiella sp. 13T. Additionally, biotreatment can be classified based on the temperature conditions into thermophilic and mesophilic microorganisms.

4. Biodesulfurization performance

There are many factors that can affect the efficiency of BDS treatment. Physical factors include operation conditions, such as pressure, temperature, time, and mixing rate. Chemical factors include the medium used to create an appropriate environment for the microorganisms. Biological factors are represented by the presence and concentration of the added microorganisms, which can be either thermophilic or mesophilic, such as Paenibacillus Spp. and Rhodococcus erythropolis, respectively. Microorganisms can also be used under either aerobic or anaerobic conditions, such as Gordonia IITR 100 and Desulfovibrio desulfuricans M6, respectively. Moreover, microorganisms can be used in different forms, including pure or single microorganisms, multiple bacterial bio-mixtures with known presence ratios, and isolated colonies. It is important to note that certain bacterial isolates, such as Rhodococcus Spp., have shown a particular ability to attack heterocyclic HCSs and desulfurize derivatives of thiophene found in petroleum.

Given this objective, and given that the physical and chemical factors serve to support the biological factors, the biological factors can be considered as the primary treatment agents, and the process is referred to as biotreatment, or BDS. As a result, the BDS treatment is carried out under the influence of biotreaters.

Another important factor to consider in BDS treatment is aeration. Aerobic microorganisms depend on the presence of oxygen for their growth and metabolism, such as Arthrobacter simplex, Acidithiobacillus Spp., and Rhodococcus Spp., while anaerobic microorganisms can survive in the absence of oxygen, such as SRB and Desulfovibrio desulfuricans M6.

The efficiency of BDS is affected by various factors such as the type of microorganism used, the level of biopurity (septic, semi-septic, or aseptic), and the environmental conditions (quantity and types of nutrients available). The differences in the ratio of sources of carbon (C), nitrogen (N), and phosphorus (P) can significantly impact the efficiency of crude oil desulfurization as they affect the growth and treatment of microorganisms. The optimal ratio of carbohydrates/nitrates/phosphates in the microorganism strain and cell volume should be considered to maximize the desulfurization efficiency.

The efficiency of BDS varies depending on the microorganism used, the biopurity status, and the environmental status. The efficiency can also be affected by the specificity of microorganisms towards different types of HCS present in fossil fuels, as well as the competition for available sources of sulfur compounds. Multiple substrates can also decrease efficiency compared to a single substrate. Microorganisms have different efficiencies for desulfurizing crude oil and its derivatives due to the presence of various HCS in different fractions. However, the pure rate of BDS is generally low. To increase the bioreaction rate, electrokinetic or sonochemical fields can be used (Awadh et al., 2020; Chauhan et al., 2015; Gunam et al., 2021b; Ismail et al., 2016; Shahaby and Essam-El-din, 2017; Boshagh et al., 2014).

5. Isolation and identification of microorganisms

Microorganisms can be prepared and identified using various techniques. They can be isolated from soil contaminated with hydrocarbon fuel, the fuel itself, or through the cultivation of pure microorganisms obtained from culture collections such as ATCC and PTCC. Fermentation processes can then be used to cultivate the microorganisms.

The microorganisms were isolated by inoculating the samples onto suitable culture media and incubated under appropriate conditions in a shaker incubator. For the bacterial mixture, a suitable medium containing distilled water, microorganism seeds, a source of carbon and energy, and sulfur for adaptation was used. The growth environment for each isolate typically includes sources of energy such as carbon or iron sources, nitrogen, metals, and vitamins. Rhodococcus strains, such as R. erythropolis, have been isolated from contaminated soil using basal salts or sulfur-free medium (Jarullah, 2012). Other isolates, such as Gordonia sp., Noocaria sp., Pantoea agglomerans, Pseudomonas delafieldii, Pseudomonas aeruginosa, SRB, and Sphingomonas subarctica, have also been prepared and studied (Chen et al., 2019a, 2019b; Gunam et al., 2021a, 2021b; Saeed et al., 2022).

There are various methods available for the identification of microorganisms based on their physiological, chemotaxonomical, chemical, and biochemical properties. Gram staining is one such method that distinguishes between gram-negative and gram-positive bacteria, while other characteristics such as metabolic types, oxygen requirements, and motility can also be used for classification. For instance, Rhodococcus Spp. can be identified based on their mycolic acid, DAP acid, and cell wall sugar composition, as well as their menaquinone profile. Other methods such as fatty acid analysis, 16S rRNA gene sequencing, and PCR amplification can also be employed for microbial identification, as exemplified by studies on Rhodococcus erythropolis and Gordonia Spp. (Morales and Le Borgne, 2017; Bergey’s manual of systematic bacteriology).

Other resources for microbial identification include the Deutsche Sammlung von Microorganismen und Zellkulturen GmbH (DSMZ). In some cases, techniques such as electroporation can be used to improve the genetic manipulation of microorganisms, such as the genus Gordonia.

6. Experimental developments technics

The studies aimed to target the treatment of whole crude oil by modeling various compounds as single or mixtures of hydrocarbons (HC) and heterocyclic sulfur compounds (HCS) to represent the required treatment crude and target, especially to treat the recalcitrant HCS on hydrodesulfurization (HDS) and oxidative desulfurization (ODS).

6.1 Biocatalysts

Studies have focused on using biocatalysts or biotreaters as a means of treating crude oil, with a particular emphasis on targeting the recalcitrant HCS on HDS and ODS. Various improvements have been reported, including the use of recombinant strains, DNA (primers, operons, enzymes, and promoters), non-cell biocatalysts (monooxygenases, oxidases, and peroxidases), thermophilic enzymes, increasing the expression of key enzymes, expression of desulfurization enzymes in heterologous hosts, alternate cells for the expression of dsz genes, coexpression of dsz genes (dsz, tds, mds, sox), increasing the number of desulfurization genes present, changes in the dsz operon’s gene order, the addition of flavin reductase, modifications to the dsz operon’s promoter, rearrangements of the dsz gene cluster, alterations to translational sequences, and the use of multiple strains or wild strains of some microorganisms. These changes have been reported in various studies (Boniek et al., 2015; Calzada et al., 2011; Chauhan et al., 2015; El-Gendy and Nassar, 2018; Kawaguchi et al., 2012; Kilbane and Star, 2016; Li et al., 2019; Malani et al., 2021; Mohebali and Ball, 2016; Nazari et al., 2017; Yaqoub, 2013; Martinez et al., 2016).

Modifying the expression of enzymes or the genes involved in the process can lead to biostability, which can increase the likelihood of commercialization (Nazari et al., 2017). Modifying the enzymes involved in the pathway can also lead to the development of a more efficient and effective treatment process (Raheb et al., 2011).

6.2 Systems

The efficiency of BDS can be affected by the reactor type, such as a stirred tank reactor, airlift reactor, or bubble column bioreactor, as well as the reactor size and geometry, which can be related to the flask or bioreactor. Several studies have reported on these factors, including Chen et al. (2018), Malani et al. (2021), Martínez et al. (2016), Prasoulas et al. (2021), and Zhang et al. (2013). Amin et al. (2013) designed two vertical rotating immobilized cell reactors (VRICR) and applied Bacillus subtilis BDCC-USA-3 and Rhodococcus erythropolis ATCC 53868 with bio-emulsifier in BDS of DBT.

Process design and scale-up are crucial aspects of BDS. The process package typically involves a microorganism generator or fermenter for the vegetation step, a bioreactor for the multi-stage or series process, separation of O/W/microorganism and precipitation of sulfates using lime or salts, biocatalyst recycling, and residual oil purification units (Mohebali and Ball, 2016; Nazari et al., 2017; Speight and El-Gendy, 2017). BDS can be intensified by using various physical, chemical, and biochemical methods, such as ultrasonic irradiation (sonication) as a stirrer (Tang et al., 2013), the application of electricity (Malani et al., 2021; Yi et al., 2019), and a combination of units, such as microwave-catalytic hydrogenation, BDS-ODS-RA, and Ads-BDS (Duissenov, 2013; Mohebali and Ball, 2016). Additionally, coupling BDS with other treatment methods, such as bio-hydrotreater or adsorption, can lead to better efficiency.

6.3 Environment

Environmental factors play an important role in biodesulfurization (BDS). The selection of an appropriate medium composition is crucial, with water being the most commonly used basis for microorganism growth. The medium should be tailored to the specific requirements of the microorganism, with sources of carbon, energy, and sulfur chosen based on their metabolic needs (Duissenov, 2013; Pacheco et al., 2019). One way to enhance BDS is by controlling mass transfer limitation of oxygen and hydrogen sulfide (Mohebali and Ball, 2016), as well as reaction rate and pathways through the use of emulsifiers (Duissenov, 2013; Li and Jiang, 2013; Karimi et al., 2017; Jiang et al., 2014; Malani et al., 2021; Mohebali and Ball, 2016; Martínez et al., 2016; Martinez et al., 2015; Rocha e Silva et al., 2017). Immobilization techniques, such as using alginate beads or lignin nanoparticles, can also improve BDS efficiency (Amin, 2011; Capecchi et al., 2020; Gunam et al., 2013; Gunam et al., 2021a, 2021b; Nassar et al., 2021a, 2021b; Silva et al., 2020). Chemical catalysts and host improvers have also been used to enhance BDS (Malani et al., 2021; Silva et al., 2020; Nazari et al., 2017; Sousa et al., 2016). In addition, diagnostic GFP fusions can be employed to improve pathway efficiency.

6.4 Improvement of factors

Optimization studies in BDS often focus on identifying the best pre-treatment methods, such as isolation techniques or desulfurization conditions. For mixtures, optimization involves determining the operating conditions of BDS to achieve the desired selectivity, while for pure substrates or lumped mixtures, the aim is to determine the availability or specificity of the microorganism. Intensification can also be optimized by defining a suitable range. Several studies have investigated optimization in BDS, including Awadh et al. (2020), Boniek et al. (2015), Chen et al. (2019b), Chen et al. (2021), and Chauhan et al. (2015).

6.5 Selectivity of target sulfur compounds

One of the main objectives in biodesulfurization research is to understand the preferential attraction of each microorganism to the different types of HCS, both under aerobic and anaerobic conditions (Awadh et al., 2020; Mohebali and Ball, 2016). This information can be used to optimize the selection of microorganisms for specific HCS desulfurization processes. Table 2 provides an overview of the most significant biotechnology techniques for desulfurization of hydrocarbon fuel fractions.

Table 2. Last modern applications of high technologies.

Fraction	Microorganism	Applied High Technology	Reference
DBT derivatives	Pseudomonas Putida	Co-substrates-gene	(Martinez et al., 2015)
DBT	Pseudomonas Putida	Oxygen mass transfer	(Martínez et al., 2016)
DBT	Pseudomonas Putida	dsz cassettes	(Martínez et al., 2016)
DBT	Brevibacillus brevis BSM	Mathematical modeling	(Nassar et al., 2016)
DBT	Rhodococcus erythropolis	Nanotechnology	(Karimi et al., 2017)
		Process Design	(Roodbari et al., 2016)
DBT	E. coli	Desensitization + Overexpression	(Li et al., 2019)
DBT, NT, derivatives	-	ultrasound ODS UODS	(Yi et al., 2019)
Gas oil	Rhodococcus	Immobilizer (SPION)	(Nassar et al., 2021b)
DBT	Pseudomonas	immobilizer	(Gunam et al., 2021a)
Crude oil	Rhodococcus erythropolis IGTS8	Increase the rate of limiting steps in the pathway	(Sousa et al., 2016)

7. Theoretical development methods

Theoretical development involves both mathematical modeling and statistical optimization of the process to simulate the treatment phenomena and achieve higher quality and lower costs, respectively.

7.1 Kinetic mechanism pathway

Microorganisms have various pathways for oxidative or reductive cleavage of C-S or C-C bonds, as shown in Table 3. However, it is advisable to selectively remove the sulfur atom from the hydrocarbon (HC) structure (such as DBT) through the 4S-pathway while retaining its HC skeleton and fuel value. This produces non-toxic or less toxic compounds such as 2-HBP, 2,2′-bihydroxybiphenyl, 2-methoxybiphenyl, and 2,2′-dimethoxy-1,1′-biphenyl, which are divided into the H.C. phase (i.e., the fuel) while the sulfur is eliminated as inorganic sulfate in the aqueous phase containing the biocatalyst (Bhatia and Sharma, 2012; Bordoloi et al., 2014; El-Gendy and Nassar, 2018; Hirschler et al., 2021; Kilbane and Star, 2016; Malani et al., 2021; Mohebali and Ball, 2016).

Table 3. Metabolisms pathways of microorganisms.

Microorganism	Reference
Bond Specificity: Oxidative cleavage C-C bond, Pathway: Kodama pathway
Pseudomonas Putida	(Malani et al., 2021)
Bond Specificity: Reductive cleavage C-S bond
N/A because it requires strict maintenance under anaerobic conditions	(Malani et al., 2021)
Oxidative cleavage C-S bond, 4S pathway
Actinomycetales	(El-Gendy and Nassar, 2018)
Achromobacter	(Bordoloi et al., 2014)
Agrobacterium strain
Arthrobacter	(Ismail et al., 2016)
Anaerobic microorganism
Bacillus	((El-Gendy and Nassar, 2018), (Nezammahalleh, 2015)
Brevibacteria	(El-Gendy and Nassar, 2018), (Nassar et al., 2013), (Nassar et al., 2016)
Candida
Corynebacteria
Desulfobacterium aniline
Desulfovibrio desulfuricans
Gordonia sp.	(El-Gendy and Nassar, 2018)
Nocardia sp.
Gordonia alkanivorans RIPI90A
Klebsiella	(El-Gendy and Nassar, 2018), (Ismail et al., 2016)
Microbacterium	(El-Gendy and Nassar, 2018)
Mycobacterium strains (G3y, phlei WU-F1, phlei WU- 0103	(Ismail et al., 2016)
Nocardia sp.
Paenibacillus	(El-Gendy and Nassar, 2018)
Pseudomonas	(Ismail et al., 2016)
Rhodococcus erythropolis strains	(El-Gendy and Nassar, 2018), (Yaqoub, 2013), (Shahaby and Essam-El-din, 2017), (Alkhalili et al., 2017), (El-Gendy et al., 2014)
Rubio	(El-Gendy and Nassar, 2018)
Sphingomonas subarctica T7b	(El-Gendy and Nassar, 2018), (Gunam et al., 2021b)
Stenotrophomonas sp. strain SA21	(El-Gendy and Nassar, 2018), (Mohamed et al., 2015), (Ismail et al., 2016)
Bacillus subrilis
Bacillus strain Al 1-2
Mycobacterium sp.
Ps. aeruginosa	(AL-Faraas et al., 2015)
Paenibacillus sp.
Oxidative cleavage C-S bond, 2HBP sulfate end product pathway
Corynebacteria sp.
Oxidative cleavage C-S bond, 2HBP+ sulfate endproducts pathway

Van Afferden demonstrated the differences between BDS and biodegradation (BDG) (Van Afferden et al., 1990). The Konami kinetic model’s biodegradation mechanism can be compared to BDS, as illustrated in Figure 1. Some strains can be used in BDS, while others in BDG of oils (Bahmani et al., 2018; Hamidi et al., 2021a,b).

The 4S pathway for biodesulfurization can be represented by the following set of equations:

1- $Dibenzothiophene (DBT) + ATP + H_{2} O \to DBT monooxygenase \to DBT sulfone + AMP + PPi$
2- $DBT sulfone \to desulfinase \to 2 ‐ hydroxybiphenyl (HBP) + {SO}_{4}^{2 -}$
3- $HBP + NADH + H^{+} \to HBP dioxygenase \to 2^{'} ‐ hydroxybiphenyl ‐ 2 ‐ sulfinate (HBPS)$
4- $HBPS + H_{2} O \to HBPS sulfatase \to biphenyl ‐ 2 ‐ sulfinate (BPS) + {HSO}_{4}^{-}$

On the other hand, the Kodama pathway for biodegradation can be represented by the following set of equations:

1- $Dibenzothiophene (DBT) + O_{2} \to DBT dioxygenase \to DBT sulfone$
2- $DBT sulfone \to desulfinase \to 2 ‐ hydroxybiphenyl (HBP) + {SO}_{4}^{2 -}$
3- $HBP + O_{2} \to HBP dioxygenase ‐ 2 ‐ hydroxy ‐ 2^{'} ‐ oxobiphenyl ‐ 3 ‐ sulfonate (HOBS)$
4- $HOBS + H_{2} O \to HOBS sulfatase \to 2 ‐ hydroxy ‐ 2^{'} ‐ oxobiphenyl ‐ 3 ‐ sulfonate lactone (HOBL) + {HSO}_{4}^{-}$
5- $HOBL + H_{2} O \to HOBL hydrolase ‐ 2 ‐ hydroxy ‐ 2^{'} ‐ oxobiphenyl ‐ 3 ‐ carboxylate (HOCB)$
6- $HOCB + O_{2} \to HOCB dioxygenase ‐ 2 ‐ hydroxy ‐ 6 ‐ oxo ‐ 6 ‐ phenylhexa ‐ 2, 4 ‐ dienoate (HOPDA) + {CO}_{2}$
7- $HOPDA + H_{2} O \to HOPDA hydrolase ‐ 2 ‐ hydroxy ‐ 6 ‐ oxo ‐ 6 ‐ phenylhexa ‐ 2, 4 ‐ dienoic acid (HOPDA acid)$

7.2 Mathematical models, simulation, and optimization

Limited efforts have been made to engineer BDS due to the lack of commercialization (Malani et al., 2021). Various models have been developed to understand the BDS phenomenon, which can be classified based on the pretreated fluid (single or multiple substrates) or the system or phenomena design-based models (Abin-Fuentes et al., 2014; Maass et al., 2015; Zhang et al., 2013). Metabolism-based models (Bhatia and Sharma, 2012; Calzada et al., 2012) can also be used when considering a single substrate. Gross mathematical models, such as Monod, Haldane, or similar models, are commonly used for preliminary evaluations and to compare microbial cultures’ desulfurization efficiencies. These models need to be improved to consider the interaction between competition and inhibition (Dejaloud et al., 2019; Irani et al., 2011). A study by Kareem in 2013 modeled the BDS of real kerosene in a batch reactor (Kareem et al., 2013).

8. Qualification for industrialization and commercialization applicability

Efforts to engineer BDS have been limited, as it has not yet been commercialized (Malani et al., 2021). To achieve the required desulfurization rate and efficiency without side effects, both experimental and theoretical development are needed. This includes rapid isolation and bio-identification characterization, as well as reaching high efficiency and rate in thermotolerant conditions using available chemicals such as solvents, and reducing product toxicity. Additionally, reusability and stability of the biocatalyst are important criteria (Alkhalili et al., 2017; El-Gendy and Nassar, 2018). The reaction rate needs to be increased by 500-fold (Malani et al., 2021; Nassar et al., 2016), and minimizing OWR is crucial to reduce separation difficulties. Mathematical models such as Monod, Haldan, or similar models are useful for preliminary evaluation and comparing microbial cultures’ desulfurization efficiencies. However, these models need to consider the interaction between competence and inhibition (Dejaloud et al., 2019; Irani et al., 2011). Kareem (2013) studied the modeling of BDS of real kerosene in a batch reactor.

9. Summary of main points

• Fossil fuels’ sulfur content poses a significant challenge in meeting quality, health, safety, and environmental regulations.
• BDS is a desulfurization process used for removing sulfur from fuels and crude oil.
• The development of microorganism-based bioreactors is crucial for BDS.

Data availability

Underlying data

No data are associated with this article.

References

Abin-Fuentes A, Leung JC, Mohamed MES, et al.: Rate-limiting step analysis of the microbial desulfurization of dibenzothiophene in a model oil system. Biotechnol. Bioeng. 2014; 111(5): 876–884. PubMed Abstract | Publisher Full Text | Free Full Text
Adegunlola GA, Oloke JK, Majolagbe ON, et al.: Microbial desulphurization of crude oil using Aspergillus flavus. Eur. J. Exp. Biol. 2012; 2(2): 400–403.
Adlakha J, Singh P, Ram SK, et al.: Optimization of conditions for deep desulfurization of heavy crude oil and hydrodesulfurized diesel by Gordonia sp. IITR100. Fuel. 2016; 184: 761–769. Publisher Full Text
Akhmadullin RM, Akhmadullina AG, Agadjanyan SI, et al.: Desulphurization of petroleum products and sewage decontamination using polymeric KSM catalyst. Oil Refining & Petrochemical. 2012; 6: 10–16.
Alejandro Dinamarca M, Orellana L, Aguirre J, et al.: Biodesulfurization of dibenzothiophene and gas oil using a bioreactor containing a catalytic bed with Rhodococcus rhodochrous immobilized on silica. Biotechnol. Lett. 2014; 36: 1649–1652. Publisher Full Text
Al-Faraas AF, Al-Jailawi MH, Yahia AI: Desulfurization of dibenzothiophene by pseudomonas aeruginosa isolated from iraqi soils. Iran. J. Biotechnol. 2015; 14(1).
Al-Jailawi MH, Al-Faraas AF, Yahia AI: Isolation and identification of dibenzothiophene biodesulfurizing bacteria. Am. J. Biosci. Bioeng. 2015; 3(5): 40–46. Publisher Full Text
Al-Kazaali W, Ataei S: Optimization of Biodesulfurization of Sour heavy Crude Oil. Under Publication Process; 2022.
Alkhalili BE, Yahya A, Ibrahim N, et al.: Biodesulfurization of sour crude oil. Mod. Appl. Sci. 2017; 11(9): 104–104. Publisher Full Text
Al-Otaibi R: New methods of direct desulphurization of crude oil. J. Pet. Environ. Biotechnol. 2013; 4(6).
Alves L, Paixão SM, Pacheco R, et al.: Biodesulphurization of fossil fuels: energy, emissions and cost analysis. RSC Adv. 2015; 5(43): 34047–34057. Publisher Full Text
Amin GA: Integrated two-stage process for biodesulfurization of model oil by vertical rotating immobilized cell reactor with the bacterium Rhodococcus erythropolis. J. Pet. Environ. Biotechnol. 2011; 2(107): 2.
Amin GA, Bazaid SA, Abd El-Halim M: A Two-stage immobilized cell bioreactor with Bacillus subtilis and Rhodococcus erythropolis for the simultaneous production of biosurfactant and biodesulfurization of model oil. Pet. Sci. Technol. 2013; 31(21): 2250–2257. Publisher Full Text
Ansari D: OPEC, Saudi Arabia, and the shale revolution: Insights from equilibrium modelling and oil politics. Energy Policy. 2017; 111: 166–178. Publisher Full Text
Awad AM: Deasphalting and Desulfurization of Al-Ahdab Crude Oil. University of Technology; 2015.
Awadh M, Mahmoud H, Abed RM, et al.: Diesel-born organosulfur compounds stimulate community re-structuring in a diesel-biodesulfurizing consortium. Biotechnol. Rep. 2020; 28: e00572. PubMed Abstract | Publisher Full Text | Free Full Text
Bahmani F, Ataei SA, Mikaili MA: The effect of moisture content variation on the bioremediation of hydrocarbon contaminated soils: modeling and experimental investigation. J Environ Anal Chem. 2018; 05(2): 236. Publisher Full Text
Bergh C: Energy efficiency in the South Africa crude oil refining industry drivers, barriers and opportunities (Master’s thesis, University of Cape Town).2012.
Bhatia S, Sharma DK: Thermophilic desulfurization of dibenzothiophene and different petroleum oils by Klebsiella sp. 13T. Environ. Sci. Pollut. Res. 2012; 19: 3491–3497. PubMed Abstract | Publisher Full Text
Boniek D, Figueiredo D, dos Santos AFB , et al.: Biodesulfurization: a mini review about the immediate search for the future technology. Clean Techn. Environ. Policy. 2015; 17: 29–37. Publisher Full Text
Bordoloi NK, Rai SK, Chaudhuri MK, et al.: Deep-desulfurization of dibenzothiophene and its derivatives present in diesel oil by a newly isolated bacterium Achromobacter sp. to reduce the environmental pollution from fossil fuel combustion. Fuel Process. Technol. 2014; 119: 236–244. Publisher Full Text
Boshagh F, Mokhtarani B, Mortaheb HR: Effect of electrokinetics on biodesulfurization of the model oil by Rhodococcus erythropolis PTCC1767 and Bacillus subtilis DSMZ 3256. J. Hazard. Mater. 2014; 280: 781–787. PubMed Abstract | Publisher Full Text
Calzada J, Alcon A, Santos VE, et al.: Mixtures of Pseudomonas putida CECT 5279 cells of different ages: Optimization as biodesulfurization catalyst. Process Biochem. 2011; 46(6): 1323–1328. Publisher Full Text
Calzada J, Alcon A, Santos VE, et al.: Extended kinetic model for DBT desulfurization using Pseudomonas Putida CECT5279 in resting cells. Biochem. Eng. J. 2012; 66: 52–60. Publisher Full Text
Capecchi E, Piccinino D, Bizzarri BM, et al.: Oxidative bio-desulfurization by nanostructured peroxidase mediator system. Catalysts. 2020; 10(3): 313. Publisher Full Text
Chauhan AK, Ahmad A, Singh SP, et al.: Biodesulfurization of benzonaphthothiophene by an isolated Gordonia sp. IITR100. Int. Biodeterior. Biodegradation. 2015; 104: 105–111. Publisher Full Text
Chavan S, Kini H, Ghosal R: Process for sulfur reduction from high viscosity petroleum oils. Int. J. Environ. Sci. Dev. 2012; 3(3): 228–231. Publisher Full Text
Chen S, Sun S, Zhao C, et al.: Biodesulfurization of model oil using growing cells of Gordonia sp. SC-10. Pet. Sci. Technol. 2019a; 37(8): 907–912. Publisher Full Text
Chen S, Zang M, Li L, et al.: Efficient biodesulfurization of diesel oil by Gordonia sp. SC-10 with highly hydrophobic cell surfaces. Biochem. Eng. J. 2021; 174: 108094. Publisher Full Text
Chen S, Zhao C, Liu Q, et al.: Thermophilic biodesulfurization and its application in oil desulfurization. Appl. Microbiol. Biotechnol. 2018; 102: 9089–9103. PubMed Abstract | Publisher Full Text
Chen S, Zhao C, Liu Q, et al.: Biodesulfurization of diesel oil in oil–water two phase reaction system by Gordonia sp. SC-10. Biotechnol. Lett. 2019b; 41: 547–554. Publisher Full Text
Dantas TNC, Neto AAD, Moura MCP d A, et al.: Study of new alternatives for removal of sulfur from diesel. Brazilian J. Pet. Gas. 2014; 8(1): 15–32. Publisher Full Text
Dejaloud A, Habibi A, Vahabzadeh F, et al.: Bioenergetic aspects of dibenzothiophene desulfurization by growing cells of Ralstonia eutropha. Pollution. 2019; 5(4): 709–719.
Dejaloud A, Vahabzadeh F, Habibi A: Ralstonia eutropha as a biocatalyst for desulfurization of dibenzothiophene. Bioprocess Biosyst. Eng. 2017; 40: 969–980. PubMed Abstract | Publisher Full Text
Derikvand P, Etemadifar Z: Sulfur removal from dibenzothiophene by newly isolated Paenibacillus validus strain PD2 and process optimization in aqueous and biphasic (model-oil) systems. Pol. J. Microbiol. 2015; 64(1): 47–54. Publisher Full Text
Duissenov D: Production and processing of sour crude and natural gas-challenges due to increasing stringent regulations (Master’s thesis, Institutt for petroleumsteknologi og anvendt geofysikk).2013.
El-Gendy NS, Nassar HMN: Biodesulfurization in petroleum refining. John Wiley & Sons; 2018. Publisher Full Text
El-Gendy NS, Nassar HN, Abu Amr SS: Factorial design and response surface optimization for enhancing a biodesulfurization process. Pet. Sci. Technol. 2014; 32(14): 1669–1679. Publisher Full Text
Feng S, Lin X, Tong Y, et al.: Biodesulfurization of sulfide wastewater for elemental sulfur recovery by isolated Halothiobacillus neapolitanus in an internal airlift loop reactor. Bioresour. Technol. 2018; 264: 244–252. PubMed Abstract | Publisher Full Text
Gunam IBW, Sitepu A, Antara NS, et al.: Bacterial desulfurization of dibenzothiophene by Pseudomonas sp. strain KWN5 immobilized in alginate beads. Jurnal Teknologi. 2021a; 83(2): 107–115. Publisher Full Text
Gunam IBW, Sone T, Asano K: Biodesulfurization of the mixture of dibenzothiophene and its alkylated derivatives by Sphingomonas subarctica T7b. Indian J. Biotechnol. 2021b; 26(3): 122–127. Publisher Full Text
Gunam IBW, Yamamura K, Sujaya IN, et al.: Biodesulfurization of dibenzothiophene and its derivatives using resting and immobilized cells of Sphingomonas subarctica T7b. J. Microbiol. Biotechnol. 2013; 23(4): 473–482. PubMed Abstract | Publisher Full Text
Hamad EZ, Al-Shafei EN, Al-Qahtani AS: Desulfurization of whole crude oil by solvent extraction and hydrotreating. U.S. Patent 8,343,336.2013.
Hamidi Y, Ataei SA, Sarrafi A: A highly efficient method with low energy and water consumption in biodegradation of total petroleum hydrocarbons of oily sludge. J. Environ. Manag. 2021a; 293: 112911. PubMed Abstract | Publisher Full Text
Hamidi Y, Ataei SA, Sarrafi A: Biodegradation of total petroleum hydrocarbons in oily sludge: a comparative study of biostimulation, bioaugmentation, and combination of methods. J. Chem. Technol. Biotechnol. 2021b; 96(5): 1302–1307. Publisher Full Text
Heidari M, Safekordi AA, Ghaedian M, et al.: Extraction conditions for removal of oxidized sulfur compounds in gas oil. Korean J. Chem. Eng. 2013; 30: 700–705. Publisher Full Text
Hirschler A, Carapito C, Maurer L, et al.: Biodesulfurization induces reprogramming of sulfur metabolism in Rhodococcus qingshengii IGTS8: Proteomics and untargeted metabolomics. Microbiol. Spectr. 2021; 9(2): e00692–e00621. Publisher Full Text
Irani ZA, Mehrnia MR, Yazdian F, et al.: Analysis of petroleum biodesulfurization in an airlift bioreactor using response surface methodology. Bioresour. Technol. 2011; 102(22): 10585–10591. PubMed Abstract | Publisher Full Text
Ismail W, El-Sayed WS, Abdul Raheem AS, et al.: Biocatalytic desulfurization capabilities of a mixed culture during non-destructive utilization of recalcitrant organosulfur compounds. Front. Microbiol. 2016; 7: 266. Publisher Full Text
Jarullah AT: Kinetic Modelling Simulation and Optimal Operation of Trickle Bed Reactor for Hydrotreating of Crude Oil. Kinetic Parameters Estimation of Hydrotreating Reactions in Trickle Bed Reactor (TBR) via Pilot Plant Experiments; Optimal Design and Operation of an Industrial TBR with Heat Integration and Economic Evaluation (Doctoral dissertation, University of Bradford).2012.
Jiang W, Jia H, Zheng Z, et al.: Catalytic oxidative desulfurization of fuels in acidic deep eutectic solvents with [(C 6 H 13) 3 P (C 14 H 29)] 3 PMo 12 O 40 as a catalyst. Pet. Sci. 2018; 15: 841–848. Publisher Full Text
Jiang X, Yang S, Li W: Biodesulfurization of model compounds and de-asphalted bunker oil by mixed culture. Appl. Biochem. Biotechnol. 2014; 172: 62–72. PubMed Abstract | Publisher Full Text
Kareem SA, Susu AA, Aribike DS, et al.: Modelling the biodesulfurization of kerosene in a batch reactor. International Journal of Engineering Science Invention. 2013; 2(4): 61–66.
Karimi E, Jeffryes C, Yazdian F, et al.: DBT desulfurization by decorating Rhodococcus erythropolis IGTS8 using magnetic Fe3O4 nanoparticles in a bioreactor. Eng. Life Sci. 2017; 17(5): 528–535. PubMed Abstract | Publisher Full Text | Free Full Text
Kawaguchi H, Kobayashi H, Sato K: Metabolic engineering of hydrophobic Rhodococcus opacus for biodesulfurization in oil–water biphasic reaction mixtures. J. Biosci. Bioeng. 2012; 113(3): 360–366. PubMed Abstract | Publisher Full Text
Kilbane JJ, Star B: Biodesulfurization: A model system for microbial physiology research. World J. Microbiol. Biotechnol. 2016; 32(8): 137. Publisher Full Text
Li L, Liao Y, Luo Y, et al.: Improved efficiency of the desulfurization of oil sulfur compounds in Escherichia coli using a combination of desensitization engineering and DszC overexpression. ACS Synth. Biol. 2019; 8(6): 1441–1451. PubMed Abstract | Publisher Full Text
Li W, Jiang X: Enhancement of bunker oil biodesulfurization by adding surfactant. World J. Microbiol. Biotechnol. 2013; 29: 103–108. Publisher Full Text
Maass D, Mayer DA, Moritz DE, et al.: An evaluation of kinetic models in the biodesulfurization of synthetic oil by Rhodococcus erythropolis ATCC 4277. Appl. Biochem. Biotechnol. 2015; 177: 759–770. PubMed Abstract | Publisher Full Text
Malani RS, Batghare AH, Bhasarkar JB, et al.: Kinetic modelling and process engineering aspects of biodesulfurization of liquid fuels: Review and analysis. Bioresour. Technol. Rep. 2021; 14: 100668. Publisher Full Text
Martínez I, Mohamed MES, Rozas D, et al.: Engineering synthetic bacterial consortia for enhanced desulfurization and revalorization of oil sulfur compounds. Metab. Eng. 2016; 35: 46–54. PubMed Abstract | Publisher Full Text
Martinez I, Santos VE, Alcon A, et al.: Enhancement of the biodesulfurization capacity of Pseudomonas putida CECT5279 by co-substrate addition. Process Biochem. 2015; 50(1): 119–124. Publisher Full Text
Martinez I, Santos VE, Gomez E, et al.: Biodesulfurization of dibenzothiophene by resting cells of Pseudomonas putida CECT5279: influence of the oxygen transfer rate in the scale-up from shaken flask to stirred tank reactor. J. Chem. Technol. Biotechnol. 2016; 91(1): 184–189. Publisher Full Text
Mohamed MES, Al-Yacoub ZH, Vedakumar JV: Biocatalytic desulfurization of thiophenic compounds and crude oil by newly isolated bacteria. Front. Microbiol. 2015; 6: 112. Publisher Full Text
Mohebali G, Ball AS: Biodesulfurization of diesel fuels–past, present and future perspectives. Int. Biodeterior. Biodegradation. 2016; 110: 163–180. Publisher Full Text
Morales M, Le Borgne S: Protocols for the isolation and preliminary characterization of bacteria for biodesulfurization and biodenitrogenation of petroleum-derived fuels. Hydrocarbon and Lipid Microbiology Protocols: Bioproducts, Biofuels, Biocatalysts and Facilitating Tools. 2017; 201–218. Publisher Full Text
Mujahid A, Maryam A, Afzal A, et al.: Molecularly imprinted poly (methyl methacrylate)-nickel sulfide hybrid membranes for adsorptive desulfurization of dibenzothiophene. Sep. Purif. Technol. 2020; 237: 116453. Publisher Full Text
Murarka P, Srivastava P: Biodesulfurization of petroleum wastes. Biovalorisation of Wastes to Renewable Chemicals and Biofuels. Elsevier; 2020; (pp. 165–187).
Nassar HN, Abu Amr SS, El-Gendy NS: Biodesulfurization of refractory sulfur compounds in petro-diesel by a novel hydrocarbon tolerable strain Paenibacillus glucanolyticus HN4. Environ. Sci. Pollut. Res. 2021a; 28: 8102–8116. PubMed Abstract | Publisher Full Text
Nassar HN, Ali HR, El-Gendy NS: Waste prosperity: Mandarin (Citrus reticulata) peels inspired SPION for enhancing diesel oil biodesulfurization efficiency by Rhodococcus erythropolis HN2. Fuel. 2021b; 294: 120534. Publisher Full Text
Nassar HN, Deriase SF, El-Gendy NS: Modeling the relationship between microbial biomass and total viable count for a new bacterial isolate used in biodesulfurization of petroleum and its fractions. Pet. Sci. Technol. 2016; 34(11-12): 980–985. Publisher Full Text
Nassar HN, El-Gendy NS, Mostafa YM, et al.: Desulfurization of dibenzothiophene by a novel strain Brevibacillus invocatus C19 isolated from Egyptian coke. Biosci. Biotechnol. Res. Asia. 2013; 10(1): 29–46. Publisher Full Text
Nazari F, Kefayati ME, Raheb J: The study of biological technologies for the removal of sulfur compounds. J. Sci. Islam. Repub. Iran. 2017; 28(3): 205–219.
Nezammahalleh H: Biodesulfurization of light crude oil using Bacillus subtilisWb600. Middle East. 2015; 4(3.0): 50–53. Publisher Full Text
Pacheco M, Paixao SM, Silva TP, et al.: On the road to cost-effective fossil fuel desulfurization by Gordonia alkanivorans strain 1B. RSC Adv. 2019; 9(44): 25405–25413. PubMed Abstract | Publisher Full Text | Free Full Text
Prasoulas G, Dimos K, Glekas P, et al.: Biodesulfurization of Dibenzothiophene and Its Alkylated Derivatives in a Two-Phase Bubble Column Bioreactor by Resting Cells of Rhodococcus erythropolis IGTS8. Processes. 2021; 9(11): 2064. Publisher Full Text
Qader IB, Kareem JH, Ismail HK, et al.: Novel phenolic deep eutectic solvents for desulfurisation of petrodiesel. Karbala Int. J. Mod. Sci. 2021; 7(12.10): 33640. Publisher Full Text
Raheb J: The Role of Microorganisms and Productions in Biodesulfurization of Fossiel Fuels. J. Microb. Biochem. Technol. 2016; 08: 498–502. Publisher Full Text
Raheb J, Mohebali GA, Hajipour MJ, et al.: A novel indigenous gordonia RIPI species identified with a reduction in energy consuming in the desulfurization process. Energy Sources, Part A: Recovery, Utilization, and Environmental Effects. 2011; 33(23): 2125–2131. Publisher Full Text
Rashidi S, Khosravi Nikou MR, Anvaripour B, et al.: Removal of sulfur and nitrogen compounds from diesel fuel using MSU-S. Iranian Journal of Oil and Gas Science and Technology. 2015; 4(1): 1–16.
Rocha e Silva FCP, Roque BAC, Rocha e Silva NMP, et al.: Yeasts and bacterial biosurfactants as demulsifiers for petroleum derivative in seawater emulsions. AMB Express. 2017; 7: 1–13. Publisher Full Text
Roodbari NH, Badiei A, Soleimani E, et al.: Tweens demulsification effects on heavy crude oil/water emulsion. Arab. J. Chem. 2016; 9: S806–S811. Publisher Full Text
Rossi G: The microbial desulfurization of coal. Geobiotechnology II: energy resources, subsurface technologies, organic pollutants and mining legal principles. 2014; 147–167. Publisher Full Text
Sadare OO, Obazu F, Daramola MO: Biodesulfurization of petroleum distillates—current status, opportunities and future challenges. Environments. 2017; 4(4): 85. Publisher Full Text
Saeed AAM, Ahmed GA, Al-Sarahi ATA: Biodesulfurization of Al-Masila (Hadramout–Yemen) Crude Oil using Pseudomonas aeruginosa. Humanit. Nat. Sci. J. 2022; 3(2): 165–175. Publisher Full Text
Shahaby AF, Essam-El-din KM: Desulfurization of crude oil and oil products by local isolated bacterial strains. Int. J. Curr. Microbiol. App. Sci. 2017; 6: 2695–2711. Publisher Full Text
Siddiqui SU, Ahmed K: METHODS FOR DESULFURIZATION OF CRUDE OIL-A REVIEW. Forensic Sci. Int. 2016; 28(2).
Silva TP, Alves L, Paixão SM: Effect of dibenzothiophene and its alkylated derivatives on coupled desulfurization and carotenoid production by Gordonia alkanivorans strain 1B. J. Environ. Manag. 2020; 270: 110825. Publisher Full Text
Sohrabi M, Kamyab H, Janalizadeh N, et al.: Bacterial desulfurization of organic sulfur compounds exist in fossil fuels. J. Pure Appl. Microbiol. 2012; 6(2): 717–729.
Sousa SF, Sousa JF, Barbosa AC, et al.: Improving the biodesulfurization of crude oil and derivatives: a qm/mm investigation of the catalytic mechanism of NADH-FMN oxidoreductase (DszD). Chem. A Eur. J. 2016; 120(27): 5300–5306. Publisher Full Text
Speight JG, El-Gendy NS: Introduction to petroleum biotechnology. Gulf Professional Publishing; 2017.
Srivastava VC: An evaluation of desulfurization technologies for sulfur removal from liquid fuels. RSC Adv. 2012; 2(3): 759–783. Publisher Full Text
Tang Q, Lin S, Cheng Y, et al.: Enhanced biodesulfurization of bunker oil by ultrasound pre-treatment with native microbial seeds. Biochem. Eng. J. 2013; 77: 58–65. Publisher Full Text
Van Afferden M, Schacht S, Klein J, et al.: Degradation of dibenzothiophene by Brevibacterium sp. DO. Arch. Microbiol. 1990; 153: 324–328. Publisher Full Text
Yang X, Wang S, Liu Y, et al.: A comparative study of the biodesulfurization efficiency of Acidithiobacillus ferrooxidans LY01 cells domesticated with ferrous iron and pyrite. Geomicrobiol. J. 2016; 33(6): 488–493. Publisher Full Text
Yaqoub Z: Biocatalyst Development for Biodesulfurization. United Kingdom: The University of Manchester; 2013.
Yi Z, Ma X, Song J, et al.: Investigations in enhancement biodesulfurization of model compounds by ultrasound pre-oxidation. Ultrason. Sonochem. 2019; 54: 110–120. PubMed Abstract | Publisher Full Text
Zhang SH, Chen H, Li W: Kinetic analysis of biodesulfurization of model oil containing multiple alkyl dibenzothiophenes. Appl. Microbiol. Biotechnol. 2013; 97: 2193–2200. PubMed Abstract | Publisher Full Text

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Wisam Mohammed Kareem Al-Khazaali
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Seyed Ahmad Ataei
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Saeed Khesareh
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Wisam Al-Khazaali is employee in Misan Oil Company, but the company did not fund this work.

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Al-Khazaali WMK, Ataei SA and Khesareh S. Biodesulfurization of Fossil Fuels: Analysis and Prospective [version 1; peer review: 2 approved, 3 approved with reservations]. F1000Research 2023, 12:1116 (https://doi.org/10.12688/f1000research.133427.1)

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Open Peer Review

Current Reviewer Status: ?

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ApprovedThe paper is scientifically sound in its current form and only minor, if any, improvements are suggested

Approved with reservations A number of small changes, sometimes more significant revisions are required to address specific details and improve the papers academic merit.

Not approvedFundamental flaws in the paper seriously undermine the findings and conclusions

Version 1

VERSION 1

PUBLISHED 08 Sep 2023

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Reviewer Report 08 May 2024

Jasim I. Humadi, Petroleum and Gas Refining Engineering Department, Tikrit University, Tikrit, Saladin Governorate, Iraq

Approved

https://doi.org/10.5256/f1000research.146416.r252876

The topic of this paper interested. It is observed goodly. However, It should incorporate the following corrections:
1) The abstract must be written more clear about the important highlights in bio desulfurization technology
2) In the introduction, the authors mentioned that the lest ppmw of sulfur is 15 in diesel fuel. What is the reference. The update level for sulfur content based on EPA in zero.
3)The benefits of bio desulfurization over other technologies should be provided in separated section
4)The essential bio catalysts for this technology must be observed clearly
5) The language must be enhanced
6) Add suggested mechanism as a figure based on the previous works
7) The conclusion must be improved. Mention more highlights about the technology

Enhancement of Desulfurization Process for Light Gas Oil Using New Zinc Oxide Loaded Over Alumina Nanocatalyst

Mathematical model, simulation and scale up of batch reactor used in oxidative desulfurization of kerosene[6],[7],[8].

F, Mohammed, et.al., 2022(Ref 9)
I, Jasim, et.al., 2023 (Ref 10)

I only recommend the authors to enhance the paper by more new related references. I don't say "must be" but I say "can".

Is the topic of the review discussed comprehensively in the context of the current literature?

Yes
Are all factual statements correct and adequately supported by citations?

Yes
Is the review written in accessible language?

Partly
Are the conclusions drawn appropriate in the context of the current research literature?

Yes

References

1. Humadi JI, Gheni SA, Ahmed SMR, Harvey A: Dimensionless evaluation and kinetics of rapid and ultradeep desulfurization of diesel fuel in an oscillatory baffled reactor.RSC Adv. 2022; 12 (23): 14385-14396 PubMed Abstract | Publisher Full Text
2. Humadi J, Issa Y, Aqar D, Ahmed M, et al.: Evaluation the performance of the tin (IV) oxide (SnO2 ) in the removal of sulfur compounds via oxidative-extractive desulfurization process for production an eco-friendly fuel. International Journal of Chemical Reactor Engineering. 2023; 21 (6): 727-741 Publisher Full Text
3. Jafar S, Nawaf A, Humadi J: Improving the extraction of sulfur-containing compounds from fuel using surfactant material in a digital baffle reactor. Materials Today: Proceedings. 2021; 42: 1777-1783 Publisher Full Text
4. Humadi J, Nawaf A, Jarullah A, Ahmed M, et al.: Design of new nano-catalysts and digital basket reactor for oxidative desulfurization of fuel: Experiments and modelling. Chemical Engineering Research and Design. 2023; 190: 634-650 Publisher Full Text
5. Humadi J, Gheni S, Ahmed S, Abdullah G, et al.: Fast, non-extractive, and ultradeep desulfurization of diesel in an oscillatory baffled reactor. Process Safety and Environmental Protection. 2021; 152: 178-187 Publisher Full Text
6. Hamad K, Humadi J, Abdulkareem H, Al‐Salihi S, et al.: Efficient immobilization of acids into activated carbon for high durability and continuous desulfurization of diesel fuel. Energy Science & Engineering. 2023; 11 (10): 3662-3679 Publisher Full Text
7. Humadi J, Shihab M, Ahmed G, Ahmed M, et al.: Process modeling and kinetic estimation for desulfurization of diesel fuel using nano - ZnO/Al2O3. Chemical Industry and Chemical Engineering Quarterly. 2024; 30 (2): 151-159 Publisher Full Text
8. Ali N, Majeed E, Hassan Abdul Razzaq G, Humadi J, et al.: Improvement of extractive desulfurization for Iraqi refinery atmospheric residual. Petroleum Science and Technology. 2024. 1-14 Publisher Full Text
9. F, Mohammed I. H, Jasim Adnan, Q Talal, et al.: Improvement of design synthetic nano-catalysts for performance enhancement of oxidative desulfurization using batch reactor. ResearchGate. 2022. Publisher Full Text
10. I, Jasim Ghassan, H Luay, A Mustafa, et al.: Improved Kerosene Quality with the Use of a Gamma Alumina Nanoparticles Supported Zinc Oxide Catalyst in a Digital Batch Baffled Reactor: Experiments and Process Modelling. 2023. 226-237

Competing Interests: No competing interests were disclosed.

Reviewer Expertise: Chemical engineering

I confirm that I have read this submission and believe that I have an appropriate level of expertise to confirm that it is of an acceptable scientific standard.

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Reviewer Report 28 Mar 2024

Jaykumar Bhasarkar, Laxminarayan Institute of Technology, Nagpur, Maharashtra, India

Approved with Reservations

https://doi.org/10.5256/f1000research.146416.r241257

The manuscript entitled "Biodesulfurization of Fossil Fuels: Analysis and Prospective"provide the insight into the biodesulfurization of fossil fuel, however, some modification can improve the quality of the paper.
1. In introduction part, it is recommended to give more information about the overall desulfruization process such as conventional process (hydrodesulfruization). Also it required to give brief about all thee other alternative methods for the desulfurization of fossil fuels.
2.In present manuscript, authors have not mention about the metabolic pathways followed by microorganisms. Therefore, it is essential to provide the metabolic pathway such as 4C pathway and Kodoma pathway with their merits and demerits.
3.Some more literature needs to be added to the present manuscript : [Ref 1,2,3,4]
4.In modeling section, authors are requested to provide more details about the kinetic model and inhibition models of the cell growth and substrate consumption. [refer : Ref 1].
The manuscript is mainly focused on a detailed review on bio desulfurization process. The present manuscript cover good-quality information on biodesulfurization process but it requires to provide merits and demerits of the other desulfurization processes.Therefore, some more alternative technologies need to be addressed in the present manuscript. It is essential to cite more references on alternative methodologies adopted for efficient desulfurization. It is also requested to provide the comparative table for the biodesulfurization process.

Is the topic of the review discussed comprehensively in the context of the current literature?

Yes
Are all factual statements correct and adequately supported by citations?

Partly
Is the review written in accessible language?

Yes
Are the conclusions drawn appropriate in the context of the current research literature?

Partly

References

1. Malani R, Batghare A, Bhasarkar J, Moholkar V: Kinetic modelling and process engineering aspects of biodesulfurization of liquid fuels: Review and analysis. Bioresource Technology Reports. 2021; 14. Publisher Full Text
2. Bhasarkar JB, Dikshit PK, Moholkar VS: Ultrasound assisted biodesulfurization of liquid fuel using free and immobilized cells of Rhodococcus rhodochrous MTCC 3552: A mechanistic investigation.Bioresour Technol. 2015; 187: 369-378 PubMed Abstract | Publisher Full Text
3. Agarwal M, Dikshit P, Bhasarkar J, Borah A, et al.: Physical insight into ultrasound-assisted biodesulfurization using free and immobilized cells of Rhodococcus rhodochrous MTCC 3552. Chemical Engineering Journal. 2016; 295: 254-267 Publisher Full Text
4. Bhasarkar J, Borah AJ, Goswami P, Moholkar VS: Mechanistic analysis of ultrasound assisted enzymatic desulfurization of liquid fuels using horseradish peroxidase.Bioresour Technol. 2015; 196: 88-98 PubMed Abstract | Publisher Full Text

Competing Interests: No competing interests were disclosed.

Reviewer Expertise: Nanomaterials, Desulfruization of liquid fuels, sonochemistry

I confirm that I have read this submission and believe that I have an appropriate level of expertise to confirm that it is of an acceptable scientific standard, however I have significant reservations, as outlined above.

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Reviewer Report 25 Mar 2024

Talib M. Albayati, University of Technology, Karrada, Iraq

Approved with Reservations

https://doi.org/10.5256/f1000research.146416.r227751

1. I have not found comparative study with other studies in this field.
2. Re-write the introduction part so as to give more details and mention the sulfur effect on environment.
3. In introduction give more emphasis to the fact that most of the previous research refers to petroleum products rather than crude oil.
4. The aims of the present work must be more cleared at the end of the introduction part.
5. What is the mechanism of Ionic Liquids aqueous solution for sulfur removal from actual crude oil.
6. The Ionic Liquids aqueous solution surface area is very important. The author must be discussing this point in detail because the surface area is very important factor increasing the removal rate.
7. What is the role of Ionic Liquids aqueous solution? Is it extraction solution or catalytic solution? How we can guess which one is the working?
8. What about the properties of Fuel desulfurization after treatment or removal sulfur?
9. I hope from the author to draw figure explain the relationship between the actual and predict values (Residual plots for the desulfurization).
10. What is the main conclusion from this study? The conclusion must be reduced.
11. Clarify the term "Sulfur wt %" (I assume it is the percentage of sulfur in the product oil).
12. I hope from the author of this paper to give more details about optimization method with 3D figure for the result of this paper.

Is the topic of the review discussed comprehensively in the context of the current literature?

Yes
Are all factual statements correct and adequately supported by citations?

Yes
Is the review written in accessible language?

Yes
Are the conclusions drawn appropriate in the context of the current research literature?

Yes

Competing Interests: No competing interests were disclosed.

Reviewer Expertise: Chemical Engineering

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Reviewer Report 21 Mar 2024

Iqrash Shafiq, Department of Chemical Engineering, COMSATS University Islamabad, Islamabad, Islamabad Capital Territory, Pakistan

Approved

https://doi.org/10.5256/f1000research.146416.r252874

The provided text appears to be an in-depth review article on biodesulfurization (BDS) of fossil fuels. Here's a summary of the key points covered in each section:

Abstract: The abstract provides an overview of BDS as

The provided text appears to be an in-depth review article on biodesulfurization (BDS) of fossil fuels. Here's a summary of the key points covered in each section:

Abstract: The abstract provides an overview of BDS as a promising method for treating sulfur in fossil fuels, highlighting its environmental benefits and technical efficiency.
Introduction: This section introduces the importance of fossil fuels, the need for sulfur treatment due to health, safety, and environmental concerns, and the regulations surrounding sulfur content in fuels.
Desulfurization methods: Various methods of desulfurization are discussed, including physical, chemical, and biological methods. BDS is emphasized for its efficiency and cost-effectiveness compared to other methods like hydrodesulfurization (HDS).
Susceptibility of treated oils and biodesulfurizers: Different microorganisms and their applications in BDS for treating various fractions of crude oil and model compounds are discussed.
Biodesulfurization performance: Factors affecting BDS efficiency, such as physical, chemical, and biological factors, are explored. The role of microorganisms, environmental conditions, and biocatalysts in BDS efficiency is highlighted.
Experimental developments techniques: This section covers experimental techniques and developments in BDS, including biocatalysts, reactor systems, environmental factors, and optimization studies.
Theoretical development methods: Mathematical modeling and theoretical aspects of BDS are discussed, including kinetic mechanisms, metabolic pathways of microorganisms, and mathematical models for simulating the BDS process.

Overall, the article provides a comprehensive overview of BDS, covering its methods, performance, experimental developments, and theoretical aspects. It highlights the importance of BDS as a promising method for reducing sulfur content in fossil fuels, addressing environmental concerns and regulatory requirements.

Is the topic of the review discussed comprehensively in the context of the current literature?

Yes
Are all factual statements correct and adequately supported by citations?

Yes
Is the review written in accessible language?

Yes
Are the conclusions drawn appropriate in the context of the current research literature?

Yes

Competing Interests: No competing interests were disclosed.

Reviewer Expertise: Desulfurization; Photocatalysis

I confirm that I have read this submission and believe that I have an appropriate level of expertise to confirm that it is of an acceptable scientific standard.

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Reviewer Report 09 Oct 2023

Shuiquan Chen, Shandong University of Science and Technology, Qingdao, Shandong, China

Approved with Reservations

https://doi.org/10.5256/f1000research.146416.r205148

The article summarizes the main advantages and challenges of BDS, as well as the future research directions. The article points out that BDS is an environmentally friendly, low-cost, and high-efficiency method that can achieve ultra-low sulfur diesel (ULSD) standards, but it still needs to improve the reaction rate, biocatalyst stability and reusability, and engineering and commercialization efforts. I recommend accepting it with the following major changes that can complement the text content:

Some details need to be modified, such as “B.T., DBT, DBTO2, M DBTSO2, MgSO4, BNT, DBS, 2,8 DMDBT, 2,6 DNDBT, DMDBT, thianthrene, and dibenzyl sulfide ” in page 4.
Some abbreviations are not defined in the article: e.g.: BNT, 4,6-DB-DBT, 4-HDBT, etc. please check and correct them throughout the text.
It has been more than thirty years since the first biodesulfurization bacterium was discovered, but the biodesulfurization technology has not been industrialized yet. Please summarize the existing problems in detail.

Is the topic of the review discussed comprehensively in the context of the current literature?

Yes
Are all factual statements correct and adequately supported by citations?

Yes
Is the review written in accessible language?

Yes
Are the conclusions drawn appropriate in the context of the current research literature?

Yes

Competing Interests: No competing interests were disclosed.

Reviewer Expertise: Biodesulfurization; bioremediation

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Author Response 10 Oct 2023

Wisam Al-Khazaali, Chemical Engineering Sec., Designs Dept., Projects Div., Misan Oil Company, Misan Governorate, Iraq

10 Oct 2023

Author Response

Thank you for important notes. I am doing the note.
Competing Interests: We confirm that is not competing interests.
Thank you for important notes. I am doing the note.
Thank you for important notes. I am doing the note.
Competing Interests: We confirm that is not competing interests. Close
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Author Response 13 Apr 2024

Wisam Al-Khazaali, Chemical Engineering Sec., Designs Dept., Projects Div., Misan Oil Company, Misan Governorate, Iraq

13 Apr 2024

Author Response

I confirm that I finish these suggestions from reviewer. Thank you for your cooperation.
Competing Interests: I confirm there are not conflict of interests.
I confirm that I finish these suggestions from reviewer. Thank you for your cooperation.
I confirm that I finish these suggestions from reviewer. Thank you for your cooperation.
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Author Response 10 Oct 2023

Wisam Al-Khazaali, Chemical Engineering Sec., Designs Dept., Projects Div., Misan Oil Company, Misan Governorate, Iraq

10 Oct 2023

Author Response

Thank you for important notes. I am doing the note.
Competing Interests: We confirm that is not competing interests.
Thank you for important notes. I am doing the note.
Thank you for important notes. I am doing the note.
Competing Interests: We confirm that is not competing interests. Close
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Author Response 13 Apr 2024

Wisam Al-Khazaali, Chemical Engineering Sec., Designs Dept., Projects Div., Misan Oil Company, Misan Governorate, Iraq

13 Apr 2024

Author Response

I confirm that I finish these suggestions from reviewer. Thank you for your cooperation.
Competing Interests: I confirm there are not conflict of interests.
I confirm that I finish these suggestions from reviewer. Thank you for your cooperation.
I confirm that I finish these suggestions from reviewer. Thank you for your cooperation.
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Version 1

VERSION 1 PUBLISHED 08 Sep 2023

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Shuiquan Chen, Shandong University of Science and Technology, Qingdao, China
Iqrash Shafiq, COMSATS University Islamabad, Islamabad, Pakistan
Talib M. Albayati, University of Technology, Karrada, Iraq
Jaykumar Bhasarkar, Laxminarayan Institute of Technology, Nagpur, India
Jasim I. Humadi, Tikrit University, Tikrit, Iraq

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4 Views

08 May 2024 | for Version 1

Jasim I. Humadi, Petroleum and Gas Refining Engineering Department, Tikrit University, Tikrit, Saladin Governorate, Iraq

4 Views Cite this report Responses(0)

Approved

Is the topic of the review discussed comprehensively in the context of the current literature?

Yes
Are all factual statements correct and adequately supported by citations?

Yes
Is the review written in accessible language?

Partly
Are the conclusions drawn appropriate in the context of the current research literature?

Yes

References

Competing Interests

No competing interests were disclosed.

Reviewer Expertise

Chemical engineering

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28 Mar 2024 | for Version 1

Jaykumar Bhasarkar, Laxminarayan Institute of Technology, Nagpur, Maharashtra, India

7 Views Cite this report Responses(0)

Approved With Reservations

Is the topic of the review discussed comprehensively in the context of the current literature?

Yes
Are all factual statements correct and adequately supported by citations?

Partly
Is the review written in accessible language?

Yes
Are the conclusions drawn appropriate in the context of the current research literature?

Partly

References

Competing Interests

No competing interests were disclosed.

Reviewer Expertise

Nanomaterials, Desulfruization of liquid fuels, sonochemistry

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6 Views

25 Mar 2024 | for Version 1

Talib M. Albayati, University of Technology, Karrada, Iraq

6 Views Cite this report Responses(0)

Approved With Reservations

Is the topic of the review discussed comprehensively in the context of the current literature?

Yes
Are all factual statements correct and adequately supported by citations?

Yes
Is the review written in accessible language?

Yes
Are the conclusions drawn appropriate in the context of the current research literature?

Yes

Competing Interests

No competing interests were disclosed.

Reviewer Expertise

Chemical Engineering

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8 Views

21 Mar 2024 | for Version 1

Iqrash Shafiq, Department of Chemical Engineering, COMSATS University Islamabad, Islamabad, Islamabad Capital Territory, Pakistan

8 Views Cite this report Responses(0)

Approved

The provided text appears to be an in-depth review article on biodesulfurization (BDS) of fossil fuels. Here's a summary of the key points covered in each section:

Abstract: The abstract provides an overview of BDS as a promising method for treating sulfur in fossil fuels, highlighting its environmental benefits and technical efficiency.
Introduction: This section introduces the importance of fossil fuels, the need for sulfur treatment due to health, safety, and environmental concerns, and the regulations surrounding sulfur content in fuels.
Desulfurization methods: Various methods of desulfurization are discussed, including physical, chemical, and biological methods. BDS is emphasized for its efficiency and cost-effectiveness compared to other methods like hydrodesulfurization (HDS).
Susceptibility of treated oils and biodesulfurizers: Different microorganisms and their applications in BDS for treating various fractions of crude oil and model compounds are discussed.
Biodesulfurization performance: Factors affecting BDS efficiency, such as physical, chemical, and biological factors, are explored. The role of microorganisms, environmental conditions, and biocatalysts in BDS efficiency is highlighted.
Experimental developments techniques: This section covers experimental techniques and developments in BDS, including biocatalysts, reactor systems, environmental factors, and optimization studies.
Theoretical development methods: Mathematical modeling and theoretical aspects of BDS are discussed, including kinetic mechanisms, metabolic pathways of microorganisms, and mathematical models for simulating the BDS process.

Is the topic of the review discussed comprehensively in the context of the current literature?

Yes
Are all factual statements correct and adequately supported by citations?

Yes
Is the review written in accessible language?

Yes
Are the conclusions drawn appropriate in the context of the current research literature?

Yes

Competing Interests

No competing interests were disclosed.

Reviewer Expertise

Desulfurization; Photocatalysis

I confirm that I have read this submission and believe that I have an appropriate level of expertise to confirm that it is of an acceptable scientific standard.

Respond to this report

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Reviewer Report

13 Views

09 Oct 2023 | for Version 1

Shuiquan Chen, Shandong University of Science and Technology, Qingdao, Shandong, China

13 Views Cite this report Responses(2)

Approved With Reservations

Some details need to be modified, such as “B.T., DBT, DBTO2, M DBTSO2, MgSO4, BNT, DBS, 2,8 DMDBT, 2,6 DNDBT, DMDBT, thianthrene, and dibenzyl sulfide ” in page 4.
Some abbreviations are not defined in the article: e.g.: BNT, 4,6-DB-DBT, 4-HDBT, etc. please check and correct them throughout the text.
It has been more than thirty years since the first biodesulfurization bacterium was discovered, but the biodesulfurization technology has not been industrialized yet. Please summarize the existing problems in detail.

Is the topic of the review discussed comprehensively in the context of the current literature?

Yes
Are all factual statements correct and adequately supported by citations?

Yes
Is the review written in accessible language?

Yes
Are the conclusions drawn appropriate in the context of the current research literature?

Yes

Competing Interests

No competing interests were disclosed.

Reviewer Expertise

Biodesulfurization; bioremediation

Respond to this report

Responses (2)

Alongside their report, reviewers assign a status to the article:

Approved - the paper is scientifically sound in its current form and only minor, if any, improvements are suggested

Approved with reservations - A number of small changes, sometimes more significant revisions are required to address specific details and improve the papers academic merit.

Not approved - fundamental flaws in the paper seriously undermine the findings and conclusions

[1] Abin-Fuentes A, Leung JC, Mohamed MES, et al.: Rate-limiting step analysis of the microbial desulfurization of dibenzothiophene in a model oil system. Biotechnol. Bioeng. 2014; 111(5): 876–884. PubMed Abstract | Publisher Full Text | Free Full Text

[2] Adegunlola GA, Oloke JK, Majolagbe ON, et al.: Microbial desulphurization of crude oil using Aspergillus flavus. Eur. J. Exp. Biol. 2012; 2(2): 400–403.

[3] Adlakha J, Singh P, Ram SK, et al.: Optimization of conditions for deep desulfurization of heavy crude oil and hydrodesulfurized diesel by Gordonia sp. IITR100. Fuel. 2016; 184: 761–769. Publisher Full Text

[4] Akhmadullin RM, Akhmadullina AG, Agadjanyan SI, et al.: Desulphurization of petroleum products and sewage decontamination using polymeric KSM catalyst. Oil Refining & Petrochemical. 2012; 6: 10–16.

[5] Alejandro Dinamarca M, Orellana L, Aguirre J, et al.: Biodesulfurization of dibenzothiophene and gas oil using a bioreactor containing a catalytic bed with Rhodococcus rhodochrous immobilized on silica. Biotechnol. Lett. 2014; 36: 1649–1652. Publisher Full Text

[6] Al-Faraas AF, Al-Jailawi MH, Yahia AI: Desulfurization of dibenzothiophene by pseudomonas aeruginosa isolated from iraqi soils. Iran. J. Biotechnol. 2015; 14(1).

[7] Al-Jailawi MH, Al-Faraas AF, Yahia AI: Isolation and identification of dibenzothiophene biodesulfurizing bacteria. Am. J. Biosci. Bioeng. 2015; 3(5): 40–46. Publisher Full Text

[8] Al-Kazaali W, Ataei S: Optimization of Biodesulfurization of Sour heavy Crude Oil. Under Publication Process; 2022.

[9] Alkhalili BE, Yahya A, Ibrahim N, et al.: Biodesulfurization of sour crude oil. Mod. Appl. Sci. 2017; 11(9): 104–104. Publisher Full Text

[10] Al-Otaibi R: New methods of direct desulphurization of crude oil. J. Pet. Environ. Biotechnol. 2013; 4(6).

[11] Alves L, Paixão SM, Pacheco R, et al.: Biodesulphurization of fossil fuels: energy, emissions and cost analysis. RSC Adv. 2015; 5(43): 34047–34057. Publisher Full Text

[12] Amin GA: Integrated two-stage process for biodesulfurization of model oil by vertical rotating immobilized cell reactor with the bacterium Rhodococcus erythropolis. J. Pet. Environ. Biotechnol. 2011; 2(107): 2.

[13] Amin GA, Bazaid SA, Abd El-Halim M: A Two-stage immobilized cell bioreactor with Bacillus subtilis and Rhodococcus erythropolis for the simultaneous production of biosurfactant and biodesulfurization of model oil. Pet. Sci. Technol. 2013; 31(21): 2250–2257. Publisher Full Text

[14] Ansari D: OPEC, Saudi Arabia, and the shale revolution: Insights from equilibrium modelling and oil politics. Energy Policy. 2017; 111: 166–178. Publisher Full Text

[15] Awad AM: Deasphalting and Desulfurization of Al-Ahdab Crude Oil. University of Technology; 2015.

[16] Awadh M, Mahmoud H, Abed RM, et al.: Diesel-born organosulfur compounds stimulate community re-structuring in a diesel-biodesulfurizing consortium. Biotechnol. Rep. 2020; 28: e00572. PubMed Abstract | Publisher Full Text | Free Full Text

[17] Bahmani F, Ataei SA, Mikaili MA: The effect of moisture content variation on the bioremediation of hydrocarbon contaminated soils: modeling and experimental investigation. J Environ Anal Chem. 2018; 05(2): 236. Publisher Full Text

[18] Bergh C: Energy efficiency in the South Africa crude oil refining industry drivers, barriers and opportunities (Master’s thesis, University of Cape Town).2012.

[19] Bhatia S, Sharma DK: Thermophilic desulfurization of dibenzothiophene and different petroleum oils by Klebsiella sp. 13T. Environ. Sci. Pollut. Res. 2012; 19: 3491–3497. PubMed Abstract | Publisher Full Text

[20] Boniek D, Figueiredo D, dos Santos AFB , et al.: Biodesulfurization: a mini review about the immediate search for the future technology. Clean Techn. Environ. Policy. 2015; 17: 29–37. Publisher Full Text

[21] Bordoloi NK, Rai SK, Chaudhuri MK, et al.: Deep-desulfurization of dibenzothiophene and its derivatives present in diesel oil by a newly isolated bacterium Achromobacter sp. to reduce the environmental pollution from fossil fuel combustion. Fuel Process. Technol. 2014; 119: 236–244. Publisher Full Text

[22] Boshagh F, Mokhtarani B, Mortaheb HR: Effect of electrokinetics on biodesulfurization of the model oil by Rhodococcus erythropolis PTCC1767 and Bacillus subtilis DSMZ 3256. J. Hazard. Mater. 2014; 280: 781–787. PubMed Abstract | Publisher Full Text

[23] Calzada J, Alcon A, Santos VE, et al.: Mixtures of Pseudomonas putida CECT 5279 cells of different ages: Optimization as biodesulfurization catalyst. Process Biochem. 2011; 46(6): 1323–1328. Publisher Full Text

[24] Calzada J, Alcon A, Santos VE, et al.: Extended kinetic model for DBT desulfurization using Pseudomonas Putida CECT5279 in resting cells. Biochem. Eng. J. 2012; 66: 52–60. Publisher Full Text

[25] Capecchi E, Piccinino D, Bizzarri BM, et al.: Oxidative bio-desulfurization by nanostructured peroxidase mediator system. Catalysts. 2020; 10(3): 313. Publisher Full Text

[26] Chauhan AK, Ahmad A, Singh SP, et al.: Biodesulfurization of benzonaphthothiophene by an isolated Gordonia sp. IITR100. Int. Biodeterior. Biodegradation. 2015; 104: 105–111. Publisher Full Text

[27] Chavan S, Kini H, Ghosal R: Process for sulfur reduction from high viscosity petroleum oils. Int. J. Environ. Sci. Dev. 2012; 3(3): 228–231. Publisher Full Text

[28] Chen S, Sun S, Zhao C, et al.: Biodesulfurization of model oil using growing cells of Gordonia sp. SC-10. Pet. Sci. Technol. 2019a; 37(8): 907–912. Publisher Full Text

[29] Chen S, Zang M, Li L, et al.: Efficient biodesulfurization of diesel oil by Gordonia sp. SC-10 with highly hydrophobic cell surfaces. Biochem. Eng. J. 2021; 174: 108094. Publisher Full Text

[30] Chen S, Zhao C, Liu Q, et al.: Thermophilic biodesulfurization and its application in oil desulfurization. Appl. Microbiol. Biotechnol. 2018; 102: 9089–9103. PubMed Abstract | Publisher Full Text

[31] Chen S, Zhao C, Liu Q, et al.: Biodesulfurization of diesel oil in oil–water two phase reaction system by Gordonia sp. SC-10. Biotechnol. Lett. 2019b; 41: 547–554. Publisher Full Text

[32] Dantas TNC, Neto AAD, Moura MCP d A, et al.: Study of new alternatives for removal of sulfur from diesel. Brazilian J. Pet. Gas. 2014; 8(1): 15–32. Publisher Full Text

[33] Dejaloud A, Habibi A, Vahabzadeh F, et al.: Bioenergetic aspects of dibenzothiophene desulfurization by growing cells of Ralstonia eutropha. Pollution. 2019; 5(4): 709–719.

[34] Dejaloud A, Vahabzadeh F, Habibi A: Ralstonia eutropha as a biocatalyst for desulfurization of dibenzothiophene. Bioprocess Biosyst. Eng. 2017; 40: 969–980. PubMed Abstract | Publisher Full Text

[35] Derikvand P, Etemadifar Z: Sulfur removal from dibenzothiophene by newly isolated Paenibacillus validus strain PD2 and process optimization in aqueous and biphasic (model-oil) systems. Pol. J. Microbiol. 2015; 64(1): 47–54. Publisher Full Text

[36] Duissenov D: Production and processing of sour crude and natural gas-challenges due to increasing stringent regulations (Master’s thesis, Institutt for petroleumsteknologi og anvendt geofysikk).2013.

[37] El-Gendy NS, Nassar HMN: Biodesulfurization in petroleum refining. John Wiley & Sons; 2018. Publisher Full Text

[38] El-Gendy NS, Nassar HN, Abu Amr SS: Factorial design and response surface optimization for enhancing a biodesulfurization process. Pet. Sci. Technol. 2014; 32(14): 1669–1679. Publisher Full Text

[39] Feng S, Lin X, Tong Y, et al.: Biodesulfurization of sulfide wastewater for elemental sulfur recovery by isolated Halothiobacillus neapolitanus in an internal airlift loop reactor. Bioresour. Technol. 2018; 264: 244–252. PubMed Abstract | Publisher Full Text

[40] Gunam IBW, Sitepu A, Antara NS, et al.: Bacterial desulfurization of dibenzothiophene by Pseudomonas sp. strain KWN5 immobilized in alginate beads. Jurnal Teknologi. 2021a; 83(2): 107–115. Publisher Full Text

[41] Gunam IBW, Sone T, Asano K: Biodesulfurization of the mixture of dibenzothiophene and its alkylated derivatives by Sphingomonas subarctica T7b. Indian J. Biotechnol. 2021b; 26(3): 122–127. Publisher Full Text

[42] Gunam IBW, Yamamura K, Sujaya IN, et al.: Biodesulfurization of dibenzothiophene and its derivatives using resting and immobilized cells of Sphingomonas subarctica T7b. J. Microbiol. Biotechnol. 2013; 23(4): 473–482. PubMed Abstract | Publisher Full Text

[43] Hamad EZ, Al-Shafei EN, Al-Qahtani AS: Desulfurization of whole crude oil by solvent extraction and hydrotreating. U.S. Patent 8,343,336.2013.

[44] Hamidi Y, Ataei SA, Sarrafi A: A highly efficient method with low energy and water consumption in biodegradation of total petroleum hydrocarbons of oily sludge. J. Environ. Manag. 2021a; 293: 112911. PubMed Abstract | Publisher Full Text

[45] Hamidi Y, Ataei SA, Sarrafi A: Biodegradation of total petroleum hydrocarbons in oily sludge: a comparative study of biostimulation, bioaugmentation, and combination of methods. J. Chem. Technol. Biotechnol. 2021b; 96(5): 1302–1307. Publisher Full Text

[46] Heidari M, Safekordi AA, Ghaedian M, et al.: Extraction conditions for removal of oxidized sulfur compounds in gas oil. Korean J. Chem. Eng. 2013; 30: 700–705. Publisher Full Text

[47] Hirschler A, Carapito C, Maurer L, et al.: Biodesulfurization induces reprogramming of sulfur metabolism in Rhodococcus qingshengii IGTS8: Proteomics and untargeted metabolomics. Microbiol. Spectr. 2021; 9(2): e00692–e00621. Publisher Full Text

[48] Irani ZA, Mehrnia MR, Yazdian F, et al.: Analysis of petroleum biodesulfurization in an airlift bioreactor using response surface methodology. Bioresour. Technol. 2011; 102(22): 10585–10591. PubMed Abstract | Publisher Full Text

[49] Ismail W, El-Sayed WS, Abdul Raheem AS, et al.: Biocatalytic desulfurization capabilities of a mixed culture during non-destructive utilization of recalcitrant organosulfur compounds. Front. Microbiol. 2016; 7: 266. Publisher Full Text

[50] Jarullah AT: Kinetic Modelling Simulation and Optimal Operation of Trickle Bed Reactor for Hydrotreating of Crude Oil. Kinetic Parameters Estimation of Hydrotreating Reactions in Trickle Bed Reactor (TBR) via Pilot Plant Experiments; Optimal Design and Operation of an Industrial TBR with Heat Integration and Economic Evaluation (Doctoral dissertation, University of Bradford).2012.

[51] Jiang W, Jia H, Zheng Z, et al.: Catalytic oxidative desulfurization of fuels in acidic deep eutectic solvents with [(C 6 H 13) 3 P (C 14 H 29)] 3 PMo 12 O 40 as a catalyst. Pet. Sci. 2018; 15: 841–848. Publisher Full Text

[52] Jiang X, Yang S, Li W: Biodesulfurization of model compounds and de-asphalted bunker oil by mixed culture. Appl. Biochem. Biotechnol. 2014; 172: 62–72. PubMed Abstract | Publisher Full Text

[53] Kareem SA, Susu AA, Aribike DS, et al.: Modelling the biodesulfurization of kerosene in a batch reactor. International Journal of Engineering Science Invention. 2013; 2(4): 61–66.

[54] Karimi E, Jeffryes C, Yazdian F, et al.: DBT desulfurization by decorating Rhodococcus erythropolis IGTS8 using magnetic Fe3O4 nanoparticles in a bioreactor. Eng. Life Sci. 2017; 17(5): 528–535. PubMed Abstract | Publisher Full Text | Free Full Text

[55] Kawaguchi H, Kobayashi H, Sato K: Metabolic engineering of hydrophobic Rhodococcus opacus for biodesulfurization in oil–water biphasic reaction mixtures. J. Biosci. Bioeng. 2012; 113(3): 360–366. PubMed Abstract | Publisher Full Text

[56] Kilbane JJ, Star B: Biodesulfurization: A model system for microbial physiology research. World J. Microbiol. Biotechnol. 2016; 32(8): 137. Publisher Full Text

[57] Li L, Liao Y, Luo Y, et al.: Improved efficiency of the desulfurization of oil sulfur compounds in Escherichia coli using a combination of desensitization engineering and DszC overexpression. ACS Synth. Biol. 2019; 8(6): 1441–1451. PubMed Abstract | Publisher Full Text

[58] Li W, Jiang X: Enhancement of bunker oil biodesulfurization by adding surfactant. World J. Microbiol. Biotechnol. 2013; 29: 103–108. Publisher Full Text

[59] Maass D, Mayer DA, Moritz DE, et al.: An evaluation of kinetic models in the biodesulfurization of synthetic oil by Rhodococcus erythropolis ATCC 4277. Appl. Biochem. Biotechnol. 2015; 177: 759–770. PubMed Abstract | Publisher Full Text

[60] Malani RS, Batghare AH, Bhasarkar JB, et al.: Kinetic modelling and process engineering aspects of biodesulfurization of liquid fuels: Review and analysis. Bioresour. Technol. Rep. 2021; 14: 100668. Publisher Full Text

[61] Martínez I, Mohamed MES, Rozas D, et al.: Engineering synthetic bacterial consortia for enhanced desulfurization and revalorization of oil sulfur compounds. Metab. Eng. 2016; 35: 46–54. PubMed Abstract | Publisher Full Text

[62] Martinez I, Santos VE, Alcon A, et al.: Enhancement of the biodesulfurization capacity of Pseudomonas putida CECT5279 by co-substrate addition. Process Biochem. 2015; 50(1): 119–124. Publisher Full Text

[63] Martinez I, Santos VE, Gomez E, et al.: Biodesulfurization of dibenzothiophene by resting cells of Pseudomonas putida CECT5279: influence of the oxygen transfer rate in the scale-up from shaken flask to stirred tank reactor. J. Chem. Technol. Biotechnol. 2016; 91(1): 184–189. Publisher Full Text

[64] Mohamed MES, Al-Yacoub ZH, Vedakumar JV: Biocatalytic desulfurization of thiophenic compounds and crude oil by newly isolated bacteria. Front. Microbiol. 2015; 6: 112. Publisher Full Text

[65] Mohebali G, Ball AS: Biodesulfurization of diesel fuels–past, present and future perspectives. Int. Biodeterior. Biodegradation. 2016; 110: 163–180. Publisher Full Text

[66] Morales M, Le Borgne S: Protocols for the isolation and preliminary characterization of bacteria for biodesulfurization and biodenitrogenation of petroleum-derived fuels. Hydrocarbon and Lipid Microbiology Protocols: Bioproducts, Biofuels, Biocatalysts and Facilitating Tools. 2017; 201–218. Publisher Full Text

[67] Mujahid A, Maryam A, Afzal A, et al.: Molecularly imprinted poly (methyl methacrylate)-nickel sulfide hybrid membranes for adsorptive desulfurization of dibenzothiophene. Sep. Purif. Technol. 2020; 237: 116453. Publisher Full Text

[68] Murarka P, Srivastava P: Biodesulfurization of petroleum wastes. Biovalorisation of Wastes to Renewable Chemicals and Biofuels. Elsevier; 2020; (pp. 165–187).

[69] Nassar HN, Abu Amr SS, El-Gendy NS: Biodesulfurization of refractory sulfur compounds in petro-diesel by a novel hydrocarbon tolerable strain Paenibacillus glucanolyticus HN4. Environ. Sci. Pollut. Res. 2021a; 28: 8102–8116. PubMed Abstract | Publisher Full Text

[70] Nassar HN, Ali HR, El-Gendy NS: Waste prosperity: Mandarin (Citrus reticulata) peels inspired SPION for enhancing diesel oil biodesulfurization efficiency by Rhodococcus erythropolis HN2. Fuel. 2021b; 294: 120534. Publisher Full Text

[71] Nassar HN, Deriase SF, El-Gendy NS: Modeling the relationship between microbial biomass and total viable count for a new bacterial isolate used in biodesulfurization of petroleum and its fractions. Pet. Sci. Technol. 2016; 34(11-12): 980–985. Publisher Full Text

[72] Nassar HN, El-Gendy NS, Mostafa YM, et al.: Desulfurization of dibenzothiophene by a novel strain Brevibacillus invocatus C19 isolated from Egyptian coke. Biosci. Biotechnol. Res. Asia. 2013; 10(1): 29–46. Publisher Full Text

[73] Nazari F, Kefayati ME, Raheb J: The study of biological technologies for the removal of sulfur compounds. J. Sci. Islam. Repub. Iran. 2017; 28(3): 205–219.

[74] Nezammahalleh H: Biodesulfurization of light crude oil using Bacillus subtilisWb600. Middle East. 2015; 4(3.0): 50–53. Publisher Full Text

[75] Pacheco M, Paixao SM, Silva TP, et al.: On the road to cost-effective fossil fuel desulfurization by Gordonia alkanivorans strain 1B. RSC Adv. 2019; 9(44): 25405–25413. PubMed Abstract | Publisher Full Text | Free Full Text

[76] Prasoulas G, Dimos K, Glekas P, et al.: Biodesulfurization of Dibenzothiophene and Its Alkylated Derivatives in a Two-Phase Bubble Column Bioreactor by Resting Cells of Rhodococcus erythropolis IGTS8. Processes. 2021; 9(11): 2064. Publisher Full Text

[77] Qader IB, Kareem JH, Ismail HK, et al.: Novel phenolic deep eutectic solvents for desulfurisation of petrodiesel. Karbala Int. J. Mod. Sci. 2021; 7(12.10): 33640. Publisher Full Text

[78] Raheb J: The Role of Microorganisms and Productions in Biodesulfurization of Fossiel Fuels. J. Microb. Biochem. Technol. 2016; 08: 498–502. Publisher Full Text

[79] Raheb J, Mohebali GA, Hajipour MJ, et al.: A novel indigenous gordonia RIPI species identified with a reduction in energy consuming in the desulfurization process. Energy Sources, Part A: Recovery, Utilization, and Environmental Effects. 2011; 33(23): 2125–2131. Publisher Full Text

[80] Rashidi S, Khosravi Nikou MR, Anvaripour B, et al.: Removal of sulfur and nitrogen compounds from diesel fuel using MSU-S. Iranian Journal of Oil and Gas Science and Technology. 2015; 4(1): 1–16.

[81] Rocha e Silva FCP, Roque BAC, Rocha e Silva NMP, et al.: Yeasts and bacterial biosurfactants as demulsifiers for petroleum derivative in seawater emulsions. AMB Express. 2017; 7: 1–13. Publisher Full Text

[82] Roodbari NH, Badiei A, Soleimani E, et al.: Tweens demulsification effects on heavy crude oil/water emulsion. Arab. J. Chem. 2016; 9: S806–S811. Publisher Full Text

[83] Rossi G: The microbial desulfurization of coal. Geobiotechnology II: energy resources, subsurface technologies, organic pollutants and mining legal principles. 2014; 147–167. Publisher Full Text

[84] Sadare OO, Obazu F, Daramola MO: Biodesulfurization of petroleum distillates—current status, opportunities and future challenges. Environments. 2017; 4(4): 85. Publisher Full Text

[85] Saeed AAM, Ahmed GA, Al-Sarahi ATA: Biodesulfurization of Al-Masila (Hadramout–Yemen) Crude Oil using Pseudomonas aeruginosa. Humanit. Nat. Sci. J. 2022; 3(2): 165–175. Publisher Full Text

[86] Shahaby AF, Essam-El-din KM: Desulfurization of crude oil and oil products by local isolated bacterial strains. Int. J. Curr. Microbiol. App. Sci. 2017; 6: 2695–2711. Publisher Full Text

[87] Siddiqui SU, Ahmed K: METHODS FOR DESULFURIZATION OF CRUDE OIL-A REVIEW. Forensic Sci. Int. 2016; 28(2).

[88] Silva TP, Alves L, Paixão SM: Effect of dibenzothiophene and its alkylated derivatives on coupled desulfurization and carotenoid production by Gordonia alkanivorans strain 1B. J. Environ. Manag. 2020; 270: 110825. Publisher Full Text

[89] Sohrabi M, Kamyab H, Janalizadeh N, et al.: Bacterial desulfurization of organic sulfur compounds exist in fossil fuels. J. Pure Appl. Microbiol. 2012; 6(2): 717–729.

[90] Sousa SF, Sousa JF, Barbosa AC, et al.: Improving the biodesulfurization of crude oil and derivatives: a qm/mm investigation of the catalytic mechanism of NADH-FMN oxidoreductase (DszD). Chem. A Eur. J. 2016; 120(27): 5300–5306. Publisher Full Text

[91] Speight JG, El-Gendy NS: Introduction to petroleum biotechnology. Gulf Professional Publishing; 2017.

[92] Srivastava VC: An evaluation of desulfurization technologies for sulfur removal from liquid fuels. RSC Adv. 2012; 2(3): 759–783. Publisher Full Text

[93] Tang Q, Lin S, Cheng Y, et al.: Enhanced biodesulfurization of bunker oil by ultrasound pre-treatment with native microbial seeds. Biochem. Eng. J. 2013; 77: 58–65. Publisher Full Text

[94] Van Afferden M, Schacht S, Klein J, et al.: Degradation of dibenzothiophene by Brevibacterium sp. DO. Arch. Microbiol. 1990; 153: 324–328. Publisher Full Text

[95] Yang X, Wang S, Liu Y, et al.: A comparative study of the biodesulfurization efficiency of Acidithiobacillus ferrooxidans LY01 cells domesticated with ferrous iron and pyrite. Geomicrobiol. J. 2016; 33(6): 488–493. Publisher Full Text

[96] Yaqoub Z: Biocatalyst Development for Biodesulfurization. United Kingdom: The University of Manchester; 2013.

[97] Yi Z, Ma X, Song J, et al.: Investigations in enhancement biodesulfurization of model compounds by ultrasound pre-oxidation. Ultrason. Sonochem. 2019; 54: 110–120. PubMed Abstract | Publisher Full Text

[98] Zhang SH, Chen H, Li W: Kinetic analysis of biodesulfurization of model oil containing multiple alkyl dibenzothiophenes. Appl. Microbiol. Biotechnol. 2013; 97: 2193–2200. PubMed Abstract | Publisher Full Text

Biodesulfurization of Fossil Fuels: Analysis and Prospective

Abstract

Keywords

1. Introduction

2. Desulfurization methods

3. Susceptibility of treated oils and biodesulfurizers

Table 1. Last Recent treatment case studies of real and model fractions.

3.1 Aerobic conditions

3.2 Anaerobic conditions

3.3 Favored anaerobic

4. Biodesulfurization performance

5. Isolation and identification of microorganisms

6. Experimental developments technics

6.1 Biocatalysts

6.2 Systems

6.3 Environment

6.4 Improvement of factors

6.5 Selectivity of target sulfur compounds

Table 2. Last modern applications of high technologies.

7. Theoretical development methods

7.1 Kinetic mechanism pathway

Table 3. Metabolisms pathways of microorganisms.

7.2 Mathematical models, simulation, and optimization

8. Qualification for industrialization and commercialization applicability

9. Summary of main points

Data availability

Underlying data

References

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