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Systematic Review

Platelet-rich plasma as a potential therapy of cystocele: a systematic review and meta-analysis

[version 1; peer review: 1 approved with reservations]
PUBLISHED 09 Dec 2024
Author details Author details
OPEN PEER REVIEW
REVIEWER STATUS

Abstract

Abstract

Background

Platelet-rich plasma (PRP) therapy, is known for its regenerative properties. PRP may be able to help with cystocele cases. This review aims to explore how it might be applied to urogynecological cases, particularly those involving cystocele.

Methods

The present systematic review adhered to the Preferred Reporting Items for Systematic Reviews and Meta-Analysis (PRISMA) guideline 2020, Meta-Analysis of Observational Studies in Epidemiology (MOOSE) guideline. The present systematic review was registered with PROSPERO with the registered number CRD42023414366. PubMed, Science Direct, Epistemonikos, COCHRANE, Google Scholar, and ProQuest were among the databases we searched. “(PRP OR Platelet-Rich Plasma) AND (Cystocele OR Anterior Pelvic Organ Prolapse)” from January 2007 to December 2022. Based on the PICO framework (Population = Patients with Cystocele; Intervention = Reconstruction with platelet-rich plasma injection; Compare = Reconstruction only; Outcome: Recurrency of Cystocele), four investigators (AMS, PMA, EAU, RSD, and AH) independently assessed eligibility by titles and abstracts. Using the Joanna Briggs Institute Critical Appraisal tool, each author evaluated full-text articles based on the kind of articles they had received. When consensus could not be obtained, disagreements were settled by involving the supervisors (EF, EMK, and BS).

Results

A total of 8,924 studies were identified. After removing duplicates and applying eligibility criteria, two articles were included, encompassing 65 patients. In two studies, PRP injections were found and administered post-anterior colporrhaphy at the pubovesical fascia. According to these two trials, women who had anterior colporrhaphy and PRP injections required fewer reoperations.

Conclusion

PRP has the potential to be a good alternative treatment to prevent cystocele recurrence. However, it cannot be generalized to large populations due to the small number of findings. Further studies with large samples examining the efficacy and safety of the therapy are needed to prove it.

Keywords

anterior vaginal prolapse, cystocele, platelet-rich plasma, potential therapy, recurrence

Introduction

Pelvic organ prolapse (POP) refers to the descent of pelvic organs, such as the colon, small intestine, urethra, or bladder, into or beyond the vaginal opening.1 POP affects an estimated 40% of women globally, with a higher prevalence in low-income countries and increasing severity with age.2 Cystocele, the protrusion of the bladder into the vagina, is the most common type of POP, constituting 81% of the 200,000 annual POP surgeries in the United States of America (USA).3,4 Risk factors include previous pregnancies, physically demanding work, and genetic disorders.3 Kayondo et al found that 56.3% of recurrences occurred at the same site in 25.2% of patients, with 80% of these being cystoceles.5

An anterior corporal reconstruction is a treatment option for cystocele; however, it primarily reinforces the vaginal wall and does not adequately address the apical compartment.1,3 To address these deficiencies, platelet-rich plasma (PRP) therapy, known for its regenerative properties, offers a potential solution.6 PRP, which contains growth factors and cytokines, is used in orthopedic and cosmetic surgery for tissue repair and regeneration.6 In cases of POP, the use of autologous regenerative substances may facilitate the repair of connective tissue, smooth muscle, vascularization, and innervation supporting the pelvic organs.6 PRP injection aims to reinforce pelvic support structures, strengthen ligaments, and restore normal anatomical function, thereby reducing the risk of POP recurrence.6 This systematic review aimed to explore the effect of PRP in treating cystocele, an area less frequently addressed compared to orthopedic and cosmetic applications. To the best of our knowledge, the present systematic review and meta-analysis is the first to assess platelet-rich plasma as a potential alternative for preventing cystocele recurrence.

Methods

Study design and setting

The present systematic review adhered to the Preferred Reporting Items for Systematic Reviews and Meta-Analysis (PRISMA) guideline 2020, Meta-Analysis of Observational Studies in Epidemiology (MOOSE) guideline. The present systematic review was registered with PROSPERO with the registered number CRD42023414366.

Research question

Recurrence in pelvic organ prolapse that has been reconstructed surgically shows a high rate. So the incidence of re-operation also increases. This is certainly uncomfortable for patients, so the author has a research question whether there is a method or further therapy to reduce the recurrence rate of pelvic organ prolapse. Platelet Rich Plasma is known to have a good anti-inflammatory and tissue repair profile and has been used by various specialist fields. So our research question is whether PRP can be an adjunct therapy to reduce the recurrence of pelvic organ prolapse.

Eligibility criteria

Population, intervention, comparison, and outcome (PICO) framework was employed to evaluate the effect of PRP injections in treating cystocele. The population of interest includes patients diagnosed with cystocele, a condition where the bladder protrudes into the vaginal wall. The intervention being assessed is PRP injection, a regenerative therapy designed to enhance tissue repair and strengthen pelvic support structures. This intervention is compared to the standard treatment of surgical reconstruction alone. The primary outcome is the recurrence of cystocele, with the study comparing the effectiveness of PRP combined with reconstruction versus reconstruction alone in preventing cystocele recurrence. The included studies were full-text and in English or Indonesian. Reviews /Case Reports/Experimental Studies, unpublished data, and duplicate studies were excluded. References from included studies were screened for additional eligible papers.

Searching strategy

Articles were sourced from PubMed, Science Direct, Epistemonikos, COCHRANE, Google Scholar, and ProQuest for the period January 2007 to December 2022, using the keywords (PRP OR Platelet-Rich Plasma) AND (Cystocele OR Anterior Pelvic Organ Prolapse). Four investigators (A.M.S., P.M.A., E.A.U., R.S.D., and A.H.) independently assessed study eligibility based on titles and abstracts. Disagreements were resolved through consultation with supervisors (E.F., E.M.K., and B.S.).

Data extraction

Each study was reviewed for details including author, year, design, country, population sample, GPAH, intervention, PRP cycle, dose (mL), injection site, cystocele recurrence, and observation length.

Quality assessment

Each author assessed potential bias using the Joanna Briggs Institute’s critical appraisal tools (jbi.global/critical-appraisal-tools). Bias was quantified by calculating the percentage of affirmative responses to appraisal questions. A graph depicting each author’s evaluation for each article was then generated. Disagreements were resolved with input from supervisors (EF, BS, EMK, and FAR).

Statistical analysis

Licensed RStudio was employed for meta-analysis utilizing a random-effects model. The use of a random effects model was chosen so that the results could be interpreted generally outside the included sample population. Findings were reported as proportions, with heterogeneity measured using I2, Q value, Degrees of freedom (df ), Tau-squared, and Tau and p-value. Lower I2 values and p-values greater than 0.05 indicate less heterogeneity. Lower I2 values and p-values greater than 0.05 indicate less heterogeneity.

Results*

Characteristics of included studies

A total of 8,924 studies were identified. After removing duplicates and applying eligibility criteria, two articles were included, encompassing 65 patients ( Figure 1). References from these studies and citations yielded no additional eligible papers. Atilgan et al compared PRP with cystocele reconstruction (colporrhaphy), while Einarsson et al focused solely on PRP in cystocele patients without a control group.

e3ea9886-c20d-41ab-99de-f27465907ff6_figure1.gif

Figure 1. Flowchart for included studies based on PRISMA statement.

Atilgan et al conducted an observational study in Turkey with 56 women with cystoceles. Group 1 underwent anterior colporrhaphy alone, while Group 2 received reconstruction plus PRP injection. Over 48 months, group 2 showed a statistically significant greater reduction in prolapse symptoms (p = 0.002) and fewer women requiring surgery compared to group 1. In group 1, 21.4% had a symptomatic recurrence, and 17.8% needed surgery, whereas, in group 2, 7.1% had a symptomatic recurrence, and only 3.5% required repair (p = 0.0001).6 Einarsson et al conducted a pilot trial in the USA using autologous platelet gel (a PRP by-product) applied to the surgical site during anterior colporrhaphy. Among nine patients, only one (12.5%) required a second procedure after ten months.7 In all included studies, the follow-up after PRP injections was 48 months and 20 months ( Figure 2). There is a substantial difference between the research by Atilgan and Einarrson, namely the research arm used. Atilgan used a non-PRP comparator while Einarsson used a single-arm with PRP only. Therefore, a side-to-side comparison between PRP and Non-PRP can only be seen in the Atilgan study. Therefore, a meta-analysis comparing the two therapies could not be performed in this study but continued with a proportional meta-analysis of the incidence of reoperation in patients who had been treated with PRP. The next difference is that the follow-up of the study is longer in Atilgan (48 months) compared to Einarsson (20 months) so the incidence of effects or success can be more convincing by Atilgan because it uses a longer observation time.

e3ea9886-c20d-41ab-99de-f27465907ff6_figure2.gif

Figure 2. Baseline and estimate characteristics of the included studies.

Risk of bias

All studies reviewed scored above 74.5%, indicating minimal bias in the included research ( Figure 3). This assessment was found because both studies did not include confounding factors that may be involved in the study and also strategies against confounding factors were not described. Atilgan has a higher score than Einarsson because it has a comparison group and more optimal measurements than Einarsson who only used single-arm studies, but it can still be included because it discusses the same thing, namely the use of PRP in Pelvic Organ Prolapse cases.

e3ea9886-c20d-41ab-99de-f27465907ff6_figure3.gif

Figure 3. Joanna Briggs Institute's score for the included studies.

Effect of platelet-rich plasma in treating cystocele

A proportional meta-analysis was performed to evaluate the current evidence from studies that have investigated the use of PRP in cases of pelvic organ prolapse (POP). Additionally, a proportional meta-analysis was conducted to assess the incidence of reoperation following PRP therapy. The analysis revealed that reoperation occurred in 4 out of 100 patients (95% CI: 0.00-0.11, p = 0.46) who underwent reconstruction followed by PRP treatment. This suggests that while reoperation is possible, its incidence remains low. The study’s heterogeneity was minimal, as indicated by a low I2 value (0%) and a low τ2 value (0%), reflecting minimal variability between studies. However, this finding should be interpreted with caution, as it may be influenced by the small number of studies that have specifically tested PRP in POP patients. ( Figure 4).

e3ea9886-c20d-41ab-99de-f27465907ff6_figure4.gif

Figure 4. Forest plot of the effect of PRP in treating cystocele.

Discussion

From the meta-analysis, the reoperative incidence following PRP treatment was 4%. Atilgan et al reported a significant reduction in recurrence incidence (p = 0.002) and fewer reoperations in the PRP group.6 Einarsson et al observed a low reoperation rate, with only 1 of 9 women requiring a second procedure after 10 months of PRP treatment.7 Both studies indicate a low reoperation rate with anterior colporrhaphy and PRP, although Einarsson et al did not compare it to reconstruction alone.6,7

None of the studies reported significant adverse effects from PRP treatment.15 Minor side effects, such as transient erythema, edema, mild headache, itching, and brief discomfort, were noted but did not include serious complications such as scarring, infection, or allergic reactions. Patients generally resumed normal activities and returned to work the following day without the need for antibiotics.15 Additionally, allogeneic PRP was found to be immunologically safe, and studies on chronic wounds showed favorable outcomes with no significant side effects.16,17

PRP enhances fibroblast migration and proliferation, promoting connective tissue regeneration.8 In urogynecology, PRP is used for prolapse repair with favorable functional and anatomical outcomes.8 The primary benefit of PRP is its low recurrence rate. Additionally, PRP injections significantly reduce prolapse symptoms as measured by the Pelvic Floor Distress Inventory scale.8 Therefore, PRP is a promising alternative for preventing cystocele recurrence.8 Previous studies also suggested that PRP acts as a growth factor, facilitating tendon and ligament healing.8

Platelets promote angiogenesis through endothelial cell proliferation and migration.9 Previous studies have predominantly focused on PRP for tissue regeneration, highlighting its growth factors such as platelet-derived growth factor (PDGF), vascular endothelial growth factor (VEGF), insulin growth factor I (IGF-I), hepatocyte growth factor (HGF), transforming growth factor beta (TGF-b), and fibroblast growth factor (FGF), which are crucial for ligament reconstruction ( Figure 5).10,11 Recent studies have explored PRP for vaginal mesh exposure and improved fibroblast adherence and repair in vaginal prolapse surgeries.12,13 Although PRP has not been used for cystocele correction, it is proposed for injection into the pubovesical fascia to enhance support and prevent recurrence.

e3ea9886-c20d-41ab-99de-f27465907ff6_figure5.gif

Figure 5. Mechanism of platelet-rich plasma.15

To the best of our knowledge, the present systematic review and meta-analysis is the first to assess platelet-rich plasma as a potential alternative for preventing cystocele recurrence. Considering the risks associated with mesh implants, PRP injection into the pubovesical fascia during anterior vaginal wall prolapse repair may offer a viable alternative to prevent cystocele recurrence.6 Currently, PRP is reported to have no recognized side effects, with no adverse events noted during the 48-month follow-up in Atilgan et al.6 However, the optimal frequency and number of PRP sessions remain unclear and vary across studies.14 Further large-scale randomized controlled trials are needed to confirm the safety and efficacy of PRP in urogynecological procedures.6,14

The present systematic review’s applicability is limited due to the small sample size and lack of a placebo-controlled comparison. Despite these limitations, the evidence supports PRP as a promising alternative for reducing cystocele recurrence. Further observational studies, clinical trials, and preclinical research are recommended to confirm PRP’s efficacy and safety in this context.

Conclusion

Our findings indicate that PRP injections may serve as a promising alternative for preventing the recurrence of postoperative cystocele. Additionally, in female patients with cystocele, PRP injections have the potential to improve symptoms, enhance anatomical outcomes, and increase postoperative satisfaction. However, the current evidence supporting these outcomes is of low quality due to the limited number of published studies and small sample sizes. Consequently, further high-quality randomized controlled trials (RCTs) are necessary to determine whether PRP injections represent an optimal alternative.

Ethics and consent

Ethical approval and consent were not required.

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Fakhrizal E, Santoso B, Kurniawati EM et al. Platelet-rich plasma as a potential therapy of cystocele: a systematic review and meta-analysis [version 1; peer review: 1 approved with reservations]. F1000Research 2024, 13:1508 (https://doi.org/10.12688/f1000research.157123.1)
NOTE: If applicable, it is important to ensure the information in square brackets after the title is included in all citations of this article.
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Key to Reviewer Statuses VIEW
ApprovedThe paper is scientifically sound in its current form and only minor, if any, improvements are suggested
Approved with reservations A number of small changes, sometimes more significant revisions are required to address specific details and improve the papers academic merit.
Not approvedFundamental flaws in the paper seriously undermine the findings and conclusions
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Reviewer Report 18 Dec 2024
Akbar Novan Dwi Saputra, Department of Obstetrics and Gynecology, Faculty of Medicine, Public Health and Nursing, Dr. Sardjito Hospital, Universitas Gadjah Mada, Yogyakarta, Indonesia 
Approved with Reservations
VIEWS 14
  1. Are the rationale for, and objectives of, the Systematic Review clearly stated? Partly
Explanation:
  • The rationale regarding the importance of cystocele and its high recurrence rate is well
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Dwi Saputra AN. Reviewer Report For: Platelet-rich plasma as a potential therapy of cystocele: a systematic review and meta-analysis [version 1; peer review: 1 approved with reservations]. F1000Research 2024, 13:1508 (https://doi.org/10.5256/f1000research.172532.r348123)
NOTE: it is important to ensure the information in square brackets after the title is included in all citations of this article.

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VERSION 1 PUBLISHED 09 Dec 2024
Comment
Alongside their report, reviewers assign a status to the article:
Approved - the paper is scientifically sound in its current form and only minor, if any, improvements are suggested
Approved with reservations - A number of small changes, sometimes more significant revisions are required to address specific details and improve the papers academic merit.
Not approved - fundamental flaws in the paper seriously undermine the findings and conclusions
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