Keywords
Nontuberculous Mycobacterial (NTM) Disease, Clinical Profile, Prevalence, Risk Factors, Tertiary Care Hospital, Central India
This article is included in the Datta Meghe Institute of Higher Education and Research collection.
Nontuberculous Mycobacterial (NTM) diseases present unique clinical challenges and are increasingly recognised as significant contributors to respiratory and extrapulmonary morbidity. This study protocol outlines an observational and cross-sectional investigation aiming to comprehensively understand the clinical profile of NTM disease, including its prevalence, risk factors, and clinical features, in patients at a tertiary care hospital in central India.
Over two years, from July 2022 to June 2024, a convenience sample size will be recruited from TB suspects meeting inclusion criteria. Comprehensive data collection will be conducted, including demographic information, clinical history, radiological findings, microbiological test results, and risk factor assessments. Statistical methods will be applied to the collected data, including descriptive statistics, comparative analysis, risk factor assessments, and multivariate analyses.
The study aims to provide valuable insights into the prevalence and clinical manifestations of NTM disease, shedding light on the risk factors contributing to its occurrence. The statistical analyses will identify key factors associated with NTM disease and characterise its clinical and radiological features.
Nontuberculous Mycobacterial (NTM) Disease, Clinical Profile, Prevalence, Risk Factors, Tertiary Care Hospital, Central India
Nontuberculous Mycobacterial (NTM) diseases, caused by various species of mycobacteria other than Mycobacterium tuberculosis, have garnered increasing recognition for their clinical significance and impact on respiratory and extrapulmonary health. These infections present a complex clinical profile, often mimicking tuberculosis (TB) and posing diagnostic and therapeutic challenges.1 In central India, a region with a diverse healthcare landscape, understanding the clinical characteristics, prevalence, and risk factors associated with NTM diseases is essential for improving patient care, diagnosis, and management.2
NTM diseases primarily affect individuals with underlying respiratory conditions, immunosuppression, or other risk factors. Moreover, their clinical presentation can range from mild respiratory symptoms to severe, life-threatening infections. As central India grapples with a high burden of TB and diverse environmental factors, differentiating NTM diseases from TB and elucidating their clinical features become paramount for precise diagnosis and appropriate management.3
This study protocol outlines an observational and cross-sectional research endeavour, with a primary objective to comprehensively investigate the clinical profile of NTM disease in patients at a tertiary care hospital in central India. The study seeks to determine the prevalence of NTM disease among TB suspects, examine various risk factors contributing to NTM infections, and elucidate the clinical and radiological features that distinguish NTM diseases from other respiratory conditions. In doing so, the study aims to provide valuable insights into the epidemiology and clinical characteristics of NTM diseases in the specific context of central India.
The research findings can inform healthcare professionals, public health initiatives, and policy-makers, ultimately leading to better diagnostic accuracy and tailored therapeutic strategies for NTM disease. Furthermore, this study contributes to a broader understanding of pulmonary and extrapulmonary diseases in the region, underscoring the growing importance of NTM diseases in the context of public health and patient care.
This study will adopt an observational and cross-sectional design to assess the clinical profile of Nontuberculous Mycobacterial (NTM) disease in patients at a tertiary care hospital in central India (Figure 1).
The study will focus on individuals in central India who are TB suspects and fulfil the inclusion criteria for NTM disease. The study population includes pulmonary and extrapulmonary NTM disease patients, irrespective of gender, aged 12 years or older. These patients may have clinical symptoms, radiological findings, microbiological evidence suggestive of NTM disease, and those suspected of having drug-resistant TB (DR-TB).
The study will be conducted at Acharya Vinoba Bhave Rural Hospital, a tertiary care hospital in central India.
Inclusion criteria: To be eligible for inclusion in the study, patients must meet the following criteria:
1. Age: Patients should be 12 years of age or older.
2. Gender: Inclusion is irrespective of gender.
3. Clinical criteria:
• Pulmonary NTM disease: Patients should exhibit pulmonary symptoms or have nodular or cavitary opacities on chest radiograph or an HRCT scan showing multifocal bronchiectasis with multiple small nodules.
• Extrapulmonary NTM disease: Patients with disseminated disease, including skin involvement, especially following organ transplantation.
4. Microbiological criteria: Patients should have positive culture results from at least two separate expectorated sputum samples.
5. DR-TB suspects: Patients suspected of having drug-resistant TB (DR-TB).
Exclusion criteria: Patients will be excluded from the study if they meet any of the following criteria:
1. Active Tuberculosis (TB) patients: Individuals with a confirmed diagnosis of active TB will not be included in this study, as the focus is on NTM disease.
2. Declining informed consent: Patients who must provide written informed consent for participation in the study will be excluded. Informed consent is essential to ensure voluntary participation and ethical considerations.
1. Selection bias: This type of bias occurs when the study sample does not represent the target population. To mitigate selection bias, ensure that study participant recruitment is done systematically and unbiasedly, adhering to the inclusion and exclusion criteria. Random sampling or stratified sampling methods can help minimise selection bias.
2. Information bias: Information bias arises from inaccuracies in data collection, either due to measurement error or misclassification of study variables. To reduce information bias, use standardised data collection tools, train data collectors, and establish quality control measures. Additionally, consider blinding data collectors to the research hypothesis to prevent measurement bias.
3. Confounding bias: Confounding bias occurs when a third variable influences the relationship between the exposure and the outcome. Conduct a multivariate analysis to control for potential confounding variables to address confounding. This may involve stratification, matching, or including confounders as covariates in statistical models.
The data collection process for this observational and cross-sectional study on the clinical profile of Nontuberculous Mycobacterial (NTM) disease involves several structured steps to ensure precise and comprehensive information acquisition. This organised approach meets the study’s objectives while maintaining patient confidentiality and ethical standards.
Patient identification and recruitment: Patients eligible for participation, as per the inclusion criteria outlined in the study protocol, will be identified through various channels, including referrals from healthcare providers, TB clinics, and other medical facilities. These patients, who provide voluntary written informed consent, will be recruited into the study.
Informed consent: Before commencing data collection, patients will be given a clear and detailed explanation of the study’s purpose, goals, and the procedures involved. Patients will be requested to provide written informed consent, signifying their willingness to participate in the research.
Data collection tools: Specialized data collection forms and questionnaires will be developed to capture relevant information systematically. These tools will encompass sections for demographic data, medical history, clinical symptoms, radiological findings, microbiological test results, and an assessment of potential risk factors.
Data collection: Highly trained healthcare professionals, including physicians, nurses, or research assistants, will conduct in-person interviews with the study participants. These interviews will delve into various topics, such as the patients’ medical histories, symptom profiles, potential risk factors, and medication histories. Additionally, clinical examinations will assess the patient’s physical conditions, with any relevant findings meticulously recorded. Radiological data will be gathered by reviewing chest radiographs and HRCT scans to document the presence of opacities, nodules, and bronchiectasis. Microbiological data will be collected by analysing sputum samples, including culture results from at least two samples.
Quality control: To ensure the precision and reliability of the collected data, regular training sessions will be conducted for data collectors, maintaining consistency in the data collection process. A thorough data review will be performed to confirm completeness and coherence, promptly addressing any discrepancies or missing information.
Data entry: All collected data will be securely entered into a dedicated database, prioritising data integrity and privacy.
Data analysis: After the data collection phase concludes, statistical and epidemiological analyses will be conducted to fulfil the study’s objectives. This may involve determining the prevalence of NTM disease, identifying associated risk factors, and characterising the clinical profiles of NTM disease patients.
Prevalence of Annual prevalence of NTM infection = 0.5%.
p = 0.5% (As per reference article4)
D = estimated error (10%) = 3%
Minimum sample size required = 22
Descriptive statistics will offer an initial overview of the data by calculating mean, median, standard deviation, and percentages to characterise the study population. Secondly, the study will compute the prevalence of NTM disease among TB suspects, providing valuable insights into the scale of the issue. Comparative analyses will be crucial in assessing differences between groups within the study population, such as patients with pulmonary and extrapulmonary NTM disease. To explore the various risk factors associated with NTM disease, logistic regression will be used to identify significant associations. Multivariate analysis will help disentangle the simultaneous effects of multiple variables on the clinical profile of NTM disease patients. Moreover, statistical tests like Chi-Square or Fisher’s Exact Test will be employed to evaluate associations between categorical variables. T-tests or ANOVA will be used when comparing means of continuous variables. If relevant, survival analysis techniques like Kaplan-Meier curves and Cox regression will be applied to examine time-to-event data. Subgroup analyses will delve into specific characteristics within the study population. Statistical software such as R (RRID:SCR_001905), SPSS (RRID:SCR_014598), or SAS version 23 (RRID:SCR_004635) will be utilised for all these analyses and the copyright license have been obtained. Ethical considerations must be rigorously followed throughout these analyses to safeguard patient confidentiality and rights. The study’s findings will be reported comprehensively, presenting the results clearly and effectively, supporting the study’s objectives and conclusions.
The Institutional Ethics Committee of Datta Meghe Institute of Higher Education and Research (DU) has granted its approval to the study protocol (Reference number: DMIHER (DU)/IEC/2022/19. Date:15-07-2022). Before commencing the study, we will obtain written informed consent from all participants, providing them with a comprehensive explanation of the study’s objectives.
Nontuberculous mycobacterial (NTM) diseases have gained significant attention in recent years due to their impact on respiratory and extrapulmonary health. Numerous studies globally have reported an increasing prevalence of NTM diseases. The prevalence varies across regions, and India is no exception to this trend. Studies by Hoefsloot et al.5 and Prevots et al.6 have highlighted the rising prevalence of NTM diseases, particularly in areas with a high burden of TB.
One of the fundamental challenges in managing NTM diseases is their clinical resemblance to tuberculosis. This clinical mimicry often leads to misdiagnoses and delayed treatment initiation. Research by Prevots and Marras7 and Ringshausen et al.8 noted that differentiating NTM diseases from TB is vital for appropriate management.
NTM infections are not limited to a single risk factor or demographic group. The risk factors associated with NTM diseases are multifactorial and may include underlying respiratory conditions, immunosuppression, and environmental factors. Research by Kartalija et al.9 and Adjemian et al.10 has explored these risk factors and highlighted the complexity of NTM disease epidemiology.
The geographic distribution of NTM species and their clinical impact can vary widely. With its unique environmental and demographic characteristics, Central India presents a distinct context for studying NTM diseases. Research by Thomson et al.11 has shown how environmental factors play a significant role in NTM epidemiology.
Accurate diagnosis is pivotal in managing NTM diseases effectively. Misdiagnoses lead to inappropriate treatment and contribute to antibiotic resistance and increased healthcare costs. Research by Griffith et al.12 underscores the importance of accurate diagnosis in NTM disease management.
Understanding the clinical and radiological features specific to NTM diseases is crucial for distinguishing them from TB and other respiratory conditions. Research by Jeong et al.13 and Winthrop et al.14 has detailed the diverse clinical presentations and radiological findings associated with NTM infections.
No data are associated with this article.
Reporting guidelines
Zenodo: STROBE checklist for ‘Observational and cross-sectional study on clinical profile of nontuberculous mycobacterial (NTM) disease in patients at tertiary care hospital of central India’. https://zenodo.org/doi/10.5281/zenodo.10983365
Licence: CC BY 4.0 Deed|Attribution 4.0 International
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Is the rationale for, and objectives of, the study clearly described?
Yes
Is the study design appropriate for the research question?
Yes
Are sufficient details of the methods provided to allow replication by others?
Yes
Are the datasets clearly presented in a useable and accessible format?
Yes
Competing Interests: No competing interests were disclosed.
Reviewer Expertise: NTM
Is the rationale for, and objectives of, the study clearly described?
Partly
Is the study design appropriate for the research question?
Partly
Are sufficient details of the methods provided to allow replication by others?
Partly
Are the datasets clearly presented in a useable and accessible format?
Partly
References
1. Marty PK, Pathakumari B, Cox TM, Van Keulen VP, et al.: Multiparameter immunoprofiling for the diagnosis and differentiation of progressive versus nonprogressive nontuberculous mycobacterial lung disease-A pilot study.PLoS One. 2024; 19 (4): e0301659 PubMed Abstract | Publisher Full TextCompeting Interests: No competing interests were disclosed.
Reviewer Expertise: Expertise in infectious diseases that includes tuberculosis, non-tuberculosis mycobacterial diseases and Candida infections.
Alongside their report, reviewers assign a status to the article:
Invited Reviewers | ||
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Version 1 09 May 24 |
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