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Research Article

Immunogenicity of 10-valent pneumococcal conjugate vaccine among infants attending Mbagathi District Hospital, Kenya

[version 1; peer review: 2 approved with reservations]
PUBLISHED 23 Jun 2015
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Abstract

Introduction: This study aimed to determine the serum concentration of IgG antibodies as an indicator of immunogenicity, alongside the assessment of socio-demographic factors that affect IgG antibody levels in infants immunized with 10-valent pneumococcal conjugate vaccine (PCV-10) at the Mbagathi District Hospital in Kenya.
 
Materials and methods: This cross-sectional study measured serum IgG antibodies among infants who had completed a 3-dose course of PCV-10. IgG antibodies to pneumococcal serotype-specific capsular polysaccharide were measured through enzyme-linked immunosorbent assay (ELISA).
 
Results: The majority (83%) of infants who completed the required dose of pneumococcal conjugate vaccine had serum titres of pneumococcal disease- (PD) specific IgG antibodies of between 0.34 mg/dl and 0.36 mg/dl. 4% of infants had serum titres of 0.30 mg/dl to 0.33 mg/dl. The remaining 2% had IgG antibody titres of either ≤0.25 mg/dl, or between 0.25 mg/dl to 0.29 mg/dl. Additionally, there was multi-collinearity among the IgG antibody levels of the infants studied and several variables that had an effect on these levels. These included: alcohol consumption by infants’ biological mothers during pregnancy (r =.595, p ≤ 0.05); maternal diet during pregnancy (r =.137, p ≤ 0.05); breastfeeding frequency (r =.220, p ≤ 0.05); proximity to other children (r =.133, p ≤ 0.05); child hospitalization (r =.131, p ≤ 0.05) and chronic illness (r =.154, p ≤0.01).
 
Conclusion: PCV-10 is immunogenic against PD four weeks after completion of 3-doses among the infants attending the Child Welfare clinic at the Mbagathi District Hospital in Kenya. Socio-demographic factors which include consumption of alcoholic drinks by infant’s biological mother during pregnancy and study infant chronic illness negatively affect the development of PD specific IgG. A balanced maternal diet during pregnancy and a breastfeeding frequency superior to three times per day have a significant positive effect on serum pneumococcal IgG levels among infants.

Keywords

Serum concentration, IgG antibodies, PCV-10

Introduction

Streptococcus pneumoniae (pneumococcus) is still the number one cause of morbidity, among infants and the elderly globally1. It is estimated that 150.7 million cases of pneumonia occur annually among infants2. Out of these, over 30% are usually severe enough to require admission to a hospital. Pneumonia accounts for >4 million deaths annually in under-developed countries3. It also contributes 20% of the total child deaths in Kenya annually4. Although there are antibiotic interventions, deaths caused by Pneumococcal Disease (PD) still remain high.

According to the World Health Organization (WHO) over 70% of child deaths in 2008 were as a result of pneumococcal disease complications5. In developing countries, pneumococcal septicemia and meningitis account for 20% and 50% of severe PD cases, respectively6. The surge in anti-biotic resistant Streptococcus pneumoniae serotypes is an increasing global concern posing serious treatment challenges7. Because of the relatively high level of success of pneumococcal conjugate vaccines, the WHO has recommended that vaccines with broader serotype coverage be developed8.

Accessibility to a safe and universally relevant sero-type inclusive vaccine is the surest way of curbing morbidity and mortality due to pneumococcal disease9. Although the WHO has recommended inclusion of PCVs in various national infant immunization programs10, it has not been very effective for >90% of under-developed countries. This is largely due to missing Streptococcus pneumoniae serotypes known to be circulating in developing countries11. PCV-10 contains serotypes 4, 6B, 9V, 14, 18C, 19F, and 23F and 1, 5 and 7F12. Pneumococcal infections, irrespective of serotype, can be successfully treated with antibiotics, but the increasing resistance among pneumococci to antibiotics has highlighted the need for prevention, which can be achieved by vaccination13. Both 23-valent pneumococcal polysaccharide vaccine (PPV) and 10-valent pneumococcal conjugate vaccines are currently being used in Kenya14 yet no study has been done to confirm whether they are immunogenic against PD or not. PPV and PCV-10 were not formulated on the basis of exclusive serotypes found in Kenya and also despite their continued use, pneumonia remains the most killer of children in Kenya15. This has excited an interest in development of new type of vaccines that include all serotypes circulating in Kenya.

Considering the antibiotic resistance levels, prevention of pneumococcal disease early in life is necessary and thus so is the need for maternal and early infant immunization. According to the WHO, the effectiveness of pneumococcal conjugate vaccines can be evaluated on the basis of serum immunoglobulin G (IgG) levels17. A worldwide threshold level of 0.35 mg/dl after three doses at 6 weeks, 10 weeks, 14 weeks and in some cases a booster dose after 12 months has been recommended by WHO as a reference value for assessing immunogenicity of pneumococcal conjugate vaccines18.

On 14th February 2011, Kenya rolled-out the PCV-10 which is the vaccine that includes >50% of the serotypes circulating in Kenya19. Since the launch of the vaccine in February 2011, there are a rising number of children enrolling, yet it is not clear to what extent the vaccine is protective. Studies have not been conducted on the effectiveness of the pneumococcal vaccine in Kenyan children. Therefore there is still a need to evaluate the immunogenicity to determine the effectiveness of the vaccine in Kenyan children20.

S. pneumoniae has over 90 serotypes, eight of which are contained in the PCV-10 (Synflorix) currently included in the Kenya Expanded Program on Immunization (KEPI). Pneumococcus serotypes vary a lot and their epidemiology is based on age, time period and geographical area21. Currently, there is no information on the pneumococcal serotypes present in Nairobi and other pneumococcal disease endemic areas in Kenya. It is possible that they are different from those included in the vaccine, putting the effectiveness of the vaccine in question. Over 70% of global annual deaths caused by PD is among children from developing countries22. Since the launch for free public use of PCV-10 in February 2011, there is an increase in the number of children being immunized yet its safety and immunogenicity has not been established. No data have been published on the immunogenicity after each dose in a series, or on the relative immunogenicity in Kenyan children of different ages. We therefore evaluated the concentration of PD immune antibodies as indicators of immunogenicity.

Materials and methods

A cross-sectional study to evaluate PCV-10 immunogenicity among Kenyan infants was conducted between April 2013 and September 2013 among infants aged 1–12 months attending the Mbagathi District Hospital Child Welfare Care Clinic (Nairobi, Kenya). This study was funded by the National Council for Science, Technology and Innovation (NACOSTI) and ethical approval was obtained from the Kenyatta University Ethics Review Committee (ERC).

The majority of the patients treated at the hospital came from the neighbouring Kibera slum – an informal settlement. Most of these children presented with PD complications.

To determine the minimum sample size, the following formula was used: n=z2p^(1p^)m2 Sample Size (n) = 38423

A minimum of 384 infants from among those who received the vaccine were required for the study with a precision level of 5%. p^ is the assumed prevalence of pneumococcal disease among vaccinated childrenm, is the desired margin of error around the estimated prevalence, herein taken to be 5% and for 95% confidence z=1.96.

The subjects were enrolled by simple consecutive and convenient sampling. This entailed determination of the sample size as above and the enrolment of subjects as they were admitted to the clinic for administration of Vitamin A supplements. A total of 318 infants were recruited on a ‘first come, first sampled’ basis and this represented a response rate of 83%24. An equivalent number of mothers were enrolled along with their infants to investigate herd immunity.

Inclusion criteria

Infants who attended the Child Welfare Clinic of Mbagathi District Hospital for vaccination and who had completed 3-doses of PCV-10 at least 4 weeks earlier were recruited. Participating infants were aged 12 months and below. Study infants’ biological mothers were approached at the clinic for informed consent. A total of 318 biological mothers to infants who were eligible for this study were also recruited to investigate if the vaccine confers herd immunity.

Exclusion criteria

Infants with a medical condition (e.g. one that requires frequent visits to hospital) which would interfere with the assessment of the study objectives were excluded from the study. All subjects not meeting the general inclusion criteria or whose mothers refused to sign the consent form were excluded.

Informed consent

All biological mothers of the infants involved in the study were approached at the Child Welfare Clinic of Mbagathi District Hospital by the researcher as they brought their children for weight check and vitamin A administration to sign a written informed consent document. This was done after reading the document and receiving a study explanation in Kiswahili as appropriate (Appendix I, translated to English in Appendix II). They also had opportunity to ask questions relevant to the study which facilitated the informed participation in the study.

Research methodology

Structured questionnaires were formulated by the main researcher and moderated by the study supervisors. Piloting was done at the Kiambu District Hospital and corrections made before the main study. Standardized questionnaires were thereafter used to collect quantitative data from the participants’ mothers.

Collection and storage of study samples

Serum samples were collected by qualified and government registered phlebotomists working at the hospital. Coagulant-free vials were used to store the collected sample25. After child preparation (entailing the presence of the mother to ease tension), a 5ml capillary blood sample (heel stick sampling) was collected aseptically into the vials. Venous blood samples were collected from the mothers. Specimens were stored at room temperature until a clot formed (usually 15–45 minutes), then centrifuged to obtain serum specimens for assay. The serum was analysed within 24 hours then stored at 4°C. Serum samples that needed to be analysed out of this range of time were stored at -20°C. Samples were then transported to the Human Diagnostics World Laboratory on dry ice.

Serosurvey

All serum samples were analysed using standard ELISA technique26. A pneumococcal antibody threshold concentration limit of 0.35 mg/dl indicated sero-protection27. Samples with antibody levels below this value were considered as lacking sero-protection.

Data collection

Responses to the well-structured questionnaire were collected. All the study questionnaires remained in safe custody of the principal investigator to ensure confidentiality. Data on IgG levels were collected through assaying serum samples for children and mothers enrolled. Data were entered, and stored on Statistical Package for Social Scientists version 20 (SPSS) until analysed.

Ethical review

Scientific and ethical approval and authorization to conduct the study was sought from Kenyatta University (KU/R/COMM/51/37-2). Signed informed consent was sought from study participants after a clear explanation of the study and its purpose. The data collected and reported were in a form that did not allow identification of individual participants. Study numbers (Barcodes) and not personal identification was used in this study.

Statistical methods

SPSS version 20 was used28. Cross-tabulations and correlation analysis were used to generate relationships between dependent and independent variables, respectively. Ninety-five percent confidence intervals were used. Univariate analysis was done to determine the most significant variable that affects the PCV-10 vaccine immunogenicity.

Dataset 1.Immunogenicity of 10-valent pneumococcal conjugate vaccine among infants at Mbagathi District hospital.
The dependent variable was the level of IgG antibody in infant’s serum following vaccination with 10-valent pneumococcal conjugate vaccine (PCV-10). The independent variables were: infant’s age, consumption of alcohol by mother, maternal diet during pregnancy, breast feeding frequency and gap with other children. Others were child hospitalization; number of house mates, chronic illness, and whether infant had been on any prolonged form of medication35.

Results and discussion

Most of the infants (30.8%) involved in this study were aged between 6–8 months. The second largest group who constituted 24.5% of the study group were aged between 3–5 months (Figure 1).

04076256-9889-45ac-b8f6-9d9287c39d24_figure1.gif

Figure 1. The age of infants vaccinated with PCV-10 that attended CWC at Mbagathi District Hospital.

Our results show that the majority of infants (83%) presented antibody levels between 0.34–0.36 mg/dl after completion of 3-doses of PCV-10 vaccination. 8% of infants presented with 0.37–0.39 mg/dl antibody levels (mg/dl) (Table 1).

Table 1. Infant’s age and IgG antibody levels (mg/dl).

Count
 IgG ANTIBODY LEVELS (mg/dl) FOR INFANTSTotal
0.25 to 0.290.30 to 0.330.34 to 0.360.37 to 0.39<0.25
INFANTS AGE3 to 5 months234009
6 to 8 months31021093235
9 to 10 months003910554
11 to 12 months10107120
Total5 (2%)13 (4%)263 (83%)26 (8%)9 (3%)318 (100%)

This was followed by 4% of infants presenting 0.30- mg/dl to 0.33 mg/dl serum concentration of PD-specific IgG antibodies. The remainder had IgG antibody titres ranging between 0.25 mg/dl and 0.29 mg/dl and ≤0.25 mg/dl respectively.

The results from the majority of the subjects (83%) correlate with the threshold recommended by the WHO29. These data were also consistent with the data obtained from studies on the level of bacterial polysaccharide immune globulin needed to prevent pneumococcal otitis media and IPD30 among Ugandan children residing in Kampala. The findings are relatively higher than those found in the study done among Brazilian infants whose IgG antibody titres between 0.30 mg/dl to 0.36 mg/dl were ≤50% of the total subjects studied31.

The data from our study shows that use of alcoholic drinks, maternal diet during pregnancy and breastfeeding frequency affect serum antibody titres. The IgG antibody titres for subjects who consumed alcoholic drinks during pregnancy were relatively lower as compared to those who did not (Table 2). The results also show that the majority (>68%) of the study subjects had antibody titres between 0.34–0.36 mg/dl (Figure 2) which means that majority of them had developed immunity against PD. This study also found out that there was multi-collinearity between IgG antibody levels (mg/dl) for mothers and several variables. These variables were: consumption of alcoholic drinks by study infants’ biological mother (r =.595, p > 0.01); maternal diet during pregnancy (r =.137, p > 0.05); breast feeding frequency (r =.220, p > 0.01); gap with other children (r =.133, p > 0.05); chronic illness (r =.154, p > 0.01); and IgG antibody levels (mg/dl) for study infant’s biological mothers (r =.675, p > 0.01). Study by CDC shared similar results with this study particularly when comparing IgG antibody levels (mg/dl) for study infant’s biological mothers and IgG antibody levels (mg/dl) for infants. The study found out that there was a relationship between levels of reactive IgG among mothers and IgG antibody levels (mg/dl) for infants. Other studies however, did not find a significant correlation between levels of IgG among mothers and socio-demographic factors.

Table 2. Consumption of alcoholic drinks by mother and IgG antibody levels for infants biological mothers.

Count
 IgG antibody levels for infant’s biological mothersTotal
0.25 to 0.290.30 to 0.330.34 to 0.360.37 to 0.39<0.25
Consumption of alcoholic
drinks by mother
Yes022 (7%)9 (3%)0031 (10%)
no09 (3%)229 (72%)47 (15%)2 (1%)287 (90%)
Total031238472318 (100%)
04076256-9889-45ac-b8f6-9d9287c39d24_figure2.gif

Figure 2. IgG antibody levels (mg/dl) for infants who had completed three doses of PCV-10.

In addition, most of the infants in this study who were breast-fed more than five times a day and whose maternal diet was balanced during pregnancy had their IgG levels higher or equal to the recommended threshold (Table 3). It should be noted that the total percentage of infants with a serum concentration of PD-specific IgG between 0.34 mg/dl – 0.36 mg/dl was 76.1% in our study, relatively higher than the score of 24% within a six month period in a study done among infants in South Africa32. This is probably because of the Streptococcus strains included in the vaccine formulation. Out of the ten strains included in the vaccine, more than half are found in Kenya whereas these strains may not be found in South Africa33. Socio-demographic patterns may have also played a role in causing the disparity in immunogenicity of the vaccine between the two studies.

Table 3. Breastfeeding frequency and IgG antibody levels for infants biological mothers.

Count
 IgG ANTIBODY LEVELS FOR INFANTS BIOLOGICAL MOTHERSTotal
0.25 to 0.290.30 to 0.330.34 to 0.360.37 to 0.39<0.25
Breast feeding
frequency
Once per day000101
Twice per day010001
Three times a day112110033
Five times a day117102790199
More than five times
a day
00767184
Total219199971318

Conclusion

The PCV-10 vaccine was found to be immunogenic against PD four weeks after three doses among infants attending the Child Welfare Clinic at Mbagathi District Hospital in Nairobi. Socio-demographic factors which include use of alcoholic drinks by infants’ biological mothers during pregnancy and study infant chronic illness negatively affect the development of PD specific IgG. Balanced maternal diet during pregnancy, breastfeeding frequency (more than three times per day), was shown to have a significant positive effect on serum pneumococcal IgG levels among infants.

Data availability

F1000Research: Dataset 1. Immunogenicity of 10-valent pneumococcal conjugate vaccine among infants at Mbagathi District hospital, 10.5256/f1000research.6087.d4903535

Consent

Written informed consent for publication of clinical details was obtained from all participants and the mothers of the infants enrolled in the study.

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Walekhwa M, Muturi M and Bukusi E. Immunogenicity of 10-valent pneumococcal conjugate vaccine among infants attending Mbagathi District Hospital, Kenya [version 1; peer review: 2 approved with reservations]. F1000Research 2015, 4:165 (https://doi.org/10.12688/f1000research.6087.1)
NOTE: If applicable, it is important to ensure the information in square brackets after the title is included in all citations of this article.
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ApprovedThe paper is scientifically sound in its current form and only minor, if any, improvements are suggested
Approved with reservations A number of small changes, sometimes more significant revisions are required to address specific details and improve the papers academic merit.
Not approvedFundamental flaws in the paper seriously undermine the findings and conclusions
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Reviewer Report 18 Feb 2016
Paul Licciardi, Department of Paediatrics, University of Melbourne, Parkville, VIC, Australia;  Pneumococcal Research Group, Murdoch Childrens Research Institute, Royal Children's Hospital, Melbourne, VIC, Australia 
Approved with Reservations
VIEWS 5
This paper by Walekhwa and colleagues presents data on the immunogenicity of PCV10 among infants and their biological mothers attending the Mbagathi District Hospital in Kenya. This is an interesting study but I have several concerns as it is currently ... Continue reading
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Licciardi P. Reviewer Report For: Immunogenicity of 10-valent pneumococcal conjugate vaccine among infants attending Mbagathi District Hospital, Kenya [version 1; peer review: 2 approved with reservations]. F1000Research 2015, 4:165 (https://doi.org/10.5256/f1000research.6518.r12496)
NOTE: it is important to ensure the information in square brackets after the title is included in all citations of this article.
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Reviewer Report 14 Jul 2015
Bartholomew N. Ondigo, Department of Biomedical Science and Technology, Maseno University, Maseno, Kenya 
Approved with Reservations
VIEWS 14
The paper by Walekhwa et al. aimed to determine the serum concentration of IgG antibodies as an indicator of immunogenicity, alongside the assessment of socio-demographic factors that affect IgG antibody levels in infants immunized with 10-valent pneumococcal conjugate vaccine (PCV-10) ... Continue reading
CITE
CITE
HOW TO CITE THIS REPORT
Ondigo BN. Reviewer Report For: Immunogenicity of 10-valent pneumococcal conjugate vaccine among infants attending Mbagathi District Hospital, Kenya [version 1; peer review: 2 approved with reservations]. F1000Research 2015, 4:165 (https://doi.org/10.5256/f1000research.6518.r9459)
NOTE: it is important to ensure the information in square brackets after the title is included in all citations of this article.
  • Author Response 17 Sep 2015
    Michael Walekhwa, Kenyatta University, Nairobi, 810901, Kenya
    17 Sep 2015
    Author Response
    I have read through the corrections posted by Dr. Ondigo and I am incorporating in this article as appropriate.

    Thank you so much for your wonderful input.
    Competing Interests: No competing interests
COMMENTS ON THIS REPORT
  • Author Response 17 Sep 2015
    Michael Walekhwa, Kenyatta University, Nairobi, 810901, Kenya
    17 Sep 2015
    Author Response
    I have read through the corrections posted by Dr. Ondigo and I am incorporating in this article as appropriate.

    Thank you so much for your wonderful input.
    Competing Interests: No competing interests

Comments on this article Comments (0)

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Alongside their report, reviewers assign a status to the article:
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Approved with reservations - A number of small changes, sometimes more significant revisions are required to address specific details and improve the papers academic merit.
Not approved - fundamental flaws in the paper seriously undermine the findings and conclusions
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