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Method Article

Prediction of fine-tuned promoter activity from DNA sequence

[version 1; peer review: 1 approved, 2 approved with reservations]
PUBLISHED 11 Feb 2016
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This article is included in the Artificial Intelligence and Machine Learning gateway.

Abstract

The quantitative prediction of transcriptional activity of genes using promoter sequence is fundamental to the engineering of biological systems for industrial purposes and understanding the natural variation in gene expression. To catalyze the development of new algorithms for this purpose, the Dialogue on Reverse Engineering Assessment and Methods (DREAM) organized a community challenge seeking predictive models of promoter activity given normalized promoter activity data for 90 ribosomal protein promoters driving expression of a fluorescent reporter gene. By developing an unbiased modeling approach that performs an iterative search for predictive DNA sequence features using the frequencies of various k-mers, inferred DNA mechanical properties and spatial positions of promoter sequences, we achieved the best performer status in this challenge. The specific predictive features used in the model included the frequency of the nucleotide G, the length of polymeric tracts of T and TA, the frequencies of 6 distinct trinucleotides and 12 tetranucleotides, and the predicted protein deformability of the DNA sequence. Our method accurately predicted the activity of 20 natural variants of ribosomal protein promoters (Spearman correlation r = 0.73) as compared to 33 laboratory-mutated variants of the promoters (r = 0.57) in a test set that was hidden from participants. Notably, our model differed substantially from the rest in 2 main ways: i) it did not explicitly utilize transcription factor binding information implying that subtle DNA sequence features are highly associated with gene expression, and ii) it was entirely based on features extracted exclusively from the 100 bp region upstream from the translational start site demonstrating that this region encodes much of the overall promoter activity. The findings from this study have important implications for the engineering of predictable gene expression systems and the evolution of gene expression in naturally occurring biological systems.

Keywords

Promoter activity, Gene expression, Expression prediction, DREAM challenges, Machine learning, Gene regulation, DNA sequence, Transcription modeling

Introduction

Transcription is a fundamental step in the decoding of information encoded in DNA into phenotypes. Therefore, knowledge of transcriptional regulation is crucial for understanding the natural variation of gene expression15 and for the accurate engineering of predictable gene expression systems68. While transcriptional regulation is one of the most highly studied areas in biology, the ability to quantitatively predict gene expression from DNA sequence remains inadequate9,10. Knowledge of transcription factors and their cognate binding sites continues to grow and has enhanced our ability to make qualitative predictions about gene expression. For example, a number of transcription factors are now well known to be involved in differentiation of stem cells into specific cell types, leading to potentially clinically useful applications such as induced pluripotent stem cells11. Inspite of this progress, only limited quantitative predictions of gene expression are possible68,12,13. Knowledge that promoter sequences of genes encode both qualitative (e.g. when to switch a gene on and off) and quantitative properties (e.g. precise levels and noise) of gene expression is implied by the heritable nature of these attributes13,14. It is becoming increasingly clear that while transcription factors are critical in gene regulation, regulatory outputs are ultimately determined by co-operation between regulators in complex circuits1517 and with chromatin states1821. In particular, transcription factors compete for DNA binding sites with nucleosomes22,23. The information for nucleosome binding is largely encoded in the DNA sequence2427, even though in vivo nucleosome occupancy is highly dynamic25,28,29. Quantitative models of gene expression, therefore, benefit from the integration of nucleosome and transcription factor binding data10,23,30.

A key barrier to quantitative modeling of gene expression using promoter sequence has been the lack of experimental methods for accurately measuring transcript levels. DNA microarrays and RNA-seq are the most widely-used systems for measuring transcript abundance, but this measurement can reflect many effects including promoter sequence, genomic position of a gene and post-transcriptional regulation of mRNA levels by processes like mRNA degradation. In addition, microarray and RNA-seq can be affected by systematic biases arising from sequence dependent hybridization kinetics31 and sequence dependent read-depth coverage32, respectively. To overcome these limitations, approaches based on promoters fused to fluorescent reporters have been developed to generate direct, real-time measurement of promoter activity with high accuracy33. This has been applied in large libraries of synthetic bacterial promoters thereby generating new insights on combinatorial cis-regulation8. It was not until recently that the first large-scale library of naturally occurring promoters of any eukaryote fused to yellow fluorescent protein (YFP) became available30. 110 yeast ribosomal protein (RP) promoters were fused to YFP and integrated into a different strain at a fixed genomic location, hence alleviating both post-translational and genomic context related effects30. Consequently, this data set is very well poised for the computational modeling of the relationship between promoter sequence and transcription activity of a eukaryotic promoter.

To provide a fair assessment of the relationship between promoter sequence and quantitative transcript levels, the Dialogue for Reverse Engineering Assessments and Methods (DREAM) organized an open community challenge in 2011 (details of the challenge as well as an overview of participating teams is provided in reference 34), inviting participants to address this question using promoter activities of the RP promoter library that was not yet published30. Participants were provided with the activities of 90 promoters and their corresponding promoter sequences and challenged to predict the activity of 53 promoters whose activities were known only to the organizers of the challenge (Figure 1A). After a period of three months, the challenge organizers independently assessed the performance of models from 21 teams using four different statistical tests. Our team, Fighting Irish Systems Team (FIrST), attained the best performance status on the basis of a combined score by the DREAM consortium in predicting the activities of these 53 promoters (Spearman correlation between predicted and actual activities r = 0.65, P = 0.002). Our approach was built upon three key propositions: i) transcription factor binding and nucleosome binding, as well as other regulatory signals are encoded in DNA9,10,12,27, ii) if i) is true, then explicit prior knowledge of transcription factor and nucleosome binding is not a mandatory prerequisite for prediction of promoter activity if training data is available. That is, an unbiased approach that explores the associations between DNA sequence patterns and promoter activity should be able to rediscover patterns that relate to the observed activity. To do this, we used machine learning methods to iteratively explore the association between promoter activity and DNA sequence patterns in 100 bp windows of promoter sequence. We considered sequence patterns such as k-mers (k = 1 to k = 5), homopolymer stretches, nucleosome binding and three mechanical properties of DNA (bendability35, deformability36 and stiffness37). Based on iterative exploration of different machine learning models, we established that a support vector machine (SVM) was the most predictive of promoter activity based on specific sequence patterns in the 100 bp upstream of the translation start site (TrSS). Our model outperformed those which applied transcription factor binding sites of known RP promoters34, implying that other sequence patterns besides transcription factor binding sites can help in fine-tuning gene expression. Indeed, among the predictive features employed by our model were poly(dT-dA) tracts that occlude nucleosomes; these have since been applied to fine-tune gene expression beyond resolutions attainable by transcription factor site mutations38. Our study expands the understanding of sequence patterns that could potentially be useful in engineering fine-tuned gene expression.

7492ec61-8c9f-4dce-8a6d-c82b92c877bb_figure1.gif

Figure 1. Summary of the DREAM6 gene expression challenge.

(A) Training data consisted of DNA sequences for 90 yeast RP promoters whose activities were experimentally determined30,34. DNA sequences for blinded test set of 53 promoters whose activity was hidden also experimentally determined but withheld from the challenge participants was also provided. (B) Outline for strategy of modeling promoter activity. Each promoter was segmented into 100 bp non-overlapping windows with the full promoter regarded as a separate window. For each window, DNA sequence features were extracted and feature selection using a linear regression wrapper performed prior to machine learning. Performance of machine learning models trained on each window was determined in 5- and 10-fold cross-validations using Pearson correlation.

Methods

DREAM6 challenge data

The training data composed of DNA sequence for 90 yeast RP promoters with known activities and a test data set of 53 promoters was downloaded from the DREAM challenge website (https://www.synapse.org//#!Synapse:syn2820426/wiki/71012). Details of promoter construction are available from Zeevi et al. 201130 and the DREAM website. Briefly, the organizers considered the promoter region as the sequence 1200 bp upstream of a gene or until the nearest gene. Each promoter was linked to a URA3 selection marker and inserted into the same fixed genomic location of a master yeast strain containing the YFP gene. In total, 110 natural RP promoter strains and 33 strains with synthetically mutated RP promoters were constructed. As a control for experimental variation, all these strains contained a control promoter (TEF2) driving the expression of red fluorescent protein (mCherry). The mCherry, TEF2, URA3, RP promoter and YFP were all a single contiguous DNA sequence arranged in that order. Measurements of the mCherry expression levels and replicates of promoters had very low variation, enabling the distinction between any two promoters with activities differing by as little as ~ 8%. The promoter activity was determined as the amount of YFP fluorescence produced during the exponential growth phase, divided by the integral of the OD during the same period. The promoter activity measures the average amount of YFP produced from each promoter, per cell, per second during the exponential phase.

Feature extraction

Each promoter sequence was divided into 100 bp non-overlapping windows. The full promoter sequence was considered as another window. To extract information from each of the windows, we considered the frequencies of specific sequences in k-mers (k = 1 to 5), length of homopolymeric stretches DNA, mechanical properties (deformability, bendability and stiffness) and nucleosome binding. K-mer counts were performed using custom scripts. DNA mechanical properties were computed using workflows constructed in the Taverna Workbench version 2.2.053 and BioMoby web-services (accessed in August 2011) imported from the Molecular Modeling and Bioinformatics Group, Barcelona, Spain54. Bendability was estimated based on trinucleotide parameters obtained from DNase I digestion and nucleosome binding data35. Deformability was based on parameters from the analysis of protein-DNA crystallography structures36. Bending stiffness was based on bending free energy using the near-neighbor model37. Nucleosome binding was based on trinucleotide preferences55.

Feature selection

For each window, feature selection was performed using a linear regression wrapper in the WEKA machine learning toolkit version 3.456 to select feature combinations that are most predictive of promoter activity. Performance of feature combinations was tested using 5- and 10-fold cross validation.

Machine learning model exploration

Three models implemented in the WEKA toolkit56 were considered: SVM regression using sequential minimal optimization (SMO), linear regression and regression trees. Models were trained using 66% of the data and tested using 34%, and included only the features that were selected as important by the linear regression wrapper. Performance was determined using Pearson correlation between model predictions and actual promoter activities computed in R version 2.11.1. The SVM model was selected for refinement based on high performance compared to the other models.

Application of SVM model to DREAM6 test set

Promoter activities were not available to the participants of the challenge. We applied the ensemble of 501 SVMs built from 500 different training/test sets in which 80% of the data was used in training and 20% in testing and a single SVM validated by 66% training set and 34% testing sets. Each SVM model utilized the 24 features selected by a linear regression wrapper as most predictive of promoter activity. To predict activities of the DREAM6 test set, the 24 features were extracted from the upstream 100 bp sequence for each promoter. Predictions were then made using each of the SVM models and averaged to obtain the final predictions.

Validation of model by DREAM6 consortium

Predictions from the SVM ensemble were submitted through the DREAM website to the organizers for a blinded evaluation on the test set. The DREAM organizers used four statistics and corresponding P-values to evaluate the performance on the test set34. Details of the equations used for these statistics have been published separately by the DREAM6 Promoter Prediction Consortium34.

  • 1. Pearson correlation between predicted and observed activities for each model submitted: To generate a P-value for observing a Pearson correlation coefficient of the same magnitude or smaller than that of a given participant, a null distribution was generated by randomly sampling predictions from other teams and repeating this 10,000 times34.

  • 2. Spearman correlation for participant between ranks of the predicted and actual ranks of promoter activities: A P-value was then generated using a null distribution obtained from randomly sampling the predictions made by the other participants. The process was repeated 10,000 times34.

  • 3. Chi-square distance metric measuring the distance between predicted and actual promoter activities: To generate a P-value for observing a chi-square distance metric of the same magnitude or smaller than that of a given model submission, a null distribution was generated by randomly sampling predictions from other teams and repeating this 10,000 times34.

  • 4. A rank distance metric measuring the difference in ranks between predicted ranks and actual ranks of promoter activities. A P-value was generated from a null distribution obtained by randomly sampling predicted ranks from other teams, repeating this 10,000 times.

The overall score was defined as the product of the four P-values34. All these scores were computed using R version 2.11.1.

Table 1. DNA sequence features predictive of promoter activity.

DNA featureDescription
MononucleotidesFrequency of G
DinucleotidesFrequency of GT
TrinucleotidesFrequency of 6 trinucleotides
TetranucleotidesFrequency of 12 tetranucleotides
T-tractsLength of T-tracts
TA-tractsLength of TA-tracts
DNA deformabilityNegatively correlated to activity

Results

Dataset 1.Raw data for 'Prediction of fine-tuned promoter activity from DNA sequence’, Siwo et al. 2016.
README.txt contains a description of the files.

Promoter activity is highly predictable using the 100 bp upstream region from TrSS

The challenge organizers provided DNA sequences and promoter activities - the average rate of YFP production from each promoter, per cell per second, during the exponential phase - for 90 RP promoters (training set) and another set of 53 promoters whose activity was withheld from participants (test set)30. We first partitioned the promoter sequences into 100 bp non-overlapping windows, extracted specific DNA features from each window and considered the full promoter sequence as its own window (Figure 1B). The features considered were k-mers (k = 1 to 5), length of homopolymeric stretches, nucleosome positioning and DNA mechanical properties (bendability, deformability and stiffness). For each window, we performed feature selection using a linear regression wrapper, then explored three different machine learning methods (SVM, linear regression and regression trees) to learn the association between features in the window and promoter activity (Figure 1B). The performance in each window was assessed by Pearson correlation using 5- and 10-fold cross-validations on the training data. We observed very poor correlation (r « 0.5) between predicted and actual promoter activities except when using the window comprising 100 bp from the TrSS. Therefore, we focused the SVM model on this window using 23 features (Table 1) selected by the linear regression wrapper. A test of this model on 1000 randomized splits of the data (66% training and 34% testing sets) gave an average Pearson correlation of 0.78. The performance of machine learning models can be biased by the training/test data set used. Therefore, to reduce this bias, we obtained an additional 500 SVM models trained on randomly sampled sets of 80% of the data and validated on the remaining 20%. In the DREAM test set (activities for this set were withheld from participants), we used the SVM models to make predictions for each promoter. For each promoter, the predicted activity was the average of predictions across all the ensemble of SVMs based only on the 100 bp upstream of the TrSS. These predicted activities were then submitted to the DREAM consortium for evaluation34.

A total of 21 teams participated in the challenge (https://www.synapse.org//#!Synapse:syn2820426/wiki/71013). Predictions from our team had a Spearman correlation of 0.65 (P = 0.002, Figure 2A) to the actual activities, Pearson correlation of 0.65 (P = 0.003), chi-squared (χ2) distance metric of 52.62 (P = 0.508) and R2 statistic measuring the difference in ranks between predicted and actual promoter activities of 35.85 (P = 0.004). The P-values were generated from the probability of obtaining a comparable or lower performance using a null distribution in which predictions were made by randomly choosing an activity for each promoter amongst all the 21 participating teams. A combined score based on the negative logarithm (base 10) of the geometric mean of the P-values for all the 4 scores ranked our team first34 (Figure 2B), with significant P-value in three out of four of the statistical tests used for evaluation. Further, although we were not ranked first in the χ2 distance metric, our model performed the most consistently across the multiple assessment metrics, suggesting a robustness of the method. A detailed comparison of the teams was published previously by the DREAM consortium34.

7492ec61-8c9f-4dce-8a6d-c82b92c877bb_figure2.gif

Figure 2. Performance of the SVM model on validation test set by the DREAM consortium.

(A) Correlation between predicted activity by the SVM model and actual promoter activity of 53 promoters whose activity was not available to participants. (B) Performance of team FIrST relative to other 20 teams based on a combined score.

Biological significance of selected features

The final SVM models utilized only 23 features consisting of the frequencies of the mononucleotide G, dinucleotide GT, 6 different trinucleotides, 12 different tetranucleotides, length of poly(dT) and poly(dA-dT) tracts (Table 1). The relative importance of these features based on weights for the SVM models is provided (see Data availability). The feature with the highest weight was the frequency of the mononucleotide G, correlating negatively with promoter activity. For many of these features there was no clear link to underlying mechanisms of gene regulation. However, it is possible that some of the k-mers may be implicitly linked to transcription factor binding sites. That is, the combination of different k-mer features could capture the binding motifs of specific transcription factors. For example the second most important feature in the SVM was the tetranucleotide ACCC which also occurs in the Rap1 binding site motif39. In addition, frequencies of different k-mers could impact the DNA mechanical structure40. Among the features identified by the SVM model were poly(dT) and poly(dT-dA) tracts which influence the rigidity of DNA24,26, thereby directly impacting nucleosome binding. Furthermore, insertion of poly(dT-dA) sequences into promoters can be used to regulate gene expression to a finer degree and at more gradual intervals than could be attained by transcription factor binding site mutations38. Some transcription factors are also highly dependent on the ability of DNA to bend4143. In particular, TATA binding protein (TBP), which binds to the TATA box, is important for regulating the activity of RP promoters42,44,45. Another directly biologically relevant feature identified by the SVM was the deformability of DNA36,46. Promoters of low activity had more deformable DNA than those of high activity (Figure 3, P = 0.008). This was particularly evident at 40 to 60 bp from the TrSS when comparing the top 20 promoters with the highest versus those with the lowest activity (Figure 3).

7492ec61-8c9f-4dce-8a6d-c82b92c877bb_figure3.gif

Figure 3. Relationship between protein deformability of promoters and activity.

Among the top 20 promoters with extreme activities (high and low), significant deviation in deformability occurs at the -40 to -60 bp region from the TrSS (T-test P = 0.008).

Finally, some of the features may affect mRNA stability, especially given their potential location downstream of the transcription start sites (TSS). Besides sequence features in the 5’UTR that are close to the TSS could affect transcription, translation and mRNA stability.

Error profile of SVM promoter activity model

Understanding the biases in prediction accuracy could provide biological insights into promoter classes and allow for refinement of models. Therefore, we investigated relationships between the nature of the test promoters and the magnitude of prediction error made by our model. Among the 53 test promoters provided by the DREAM challenge, 20 were natural yeast RP promoters while 33 were variants of these promoters with specific synthetic mutations introduced. These mutations included changes in the binding sites of the TBP, Rap1, Fhl and Sfp1, as well as introduction of nucleosome disfavoring sequences and random mutations. At the time of the challenge, participants were not aware of these mutations. The performance of our model on the set of natural promoters was much higher (Pearson correlation r= 0.73, P = 0.0003) compared to that for the mutated promoters (Pearson correlation r= 0.57, P = 0.0005). The prediction error was significantly less for natural promoters versus the mutated promoters (Student’s t-test, P = 0.01, Figure 4A). This could partly be due to the composition of the training set, which contained only natural promoters. Similar poor performance was also observed in the models obtained from other teams34. In addition, most of the synthetic mutations were introduced at promoter locations residing outside of the 100 bp region from the TrSS and could not therefore be detected by our model. We also examined the correlation between the observed promoter activity and the prediction error. Promoters of low activity had larger prediction error (Pearson correlation between promoter activity and prediction error r= -0.31, P = 0.02, Figure 4B). Notably, natural promoters had slightly lower activity compared to synthetic promoters (P = 0.02) so the correlation between activity and prediction error may be a consequence of the low predictability of synthetic promoters. Thus, future models may benefit from data on activities of mutated promoters, which could enable a more accurate modeling of the impact of mutation on specific transcription factor binding sites.

7492ec61-8c9f-4dce-8a6d-c82b92c877bb_figure4.gif

Figure 4. Dependence of prediction error on promoter class or activity.

(A) Natural promoters had a lower prediction error compared to synthetically mutated promoters. (B) Prediction error is negatively correlated to promoter activity.

Discussion

The quantitative modeling of gene expression has the potential to enhance our understanding of how gene regulation is fine-tuned in natural populations and has implications for the design of predictable gene expression systems. The DREAM6 challenge data set for promoter activity prediction was a unique opportunity to evaluate the predictability of gene expression from its promoter sequence. Given that all promoters were derived from natural yeast RP promoters that are expressed in the exponential phase30, the challenge posed was more targeted towards DNA sequence patterns that fine-tune gene expression rather than simply determine the ‘on/off’ expression status. RP transcription regulation occurs in a highly coordinated manner and is critical for growth, allowing cells to adjust their protein synthesis capacity to physiological needs47,48. This is especially crucial as RP gene expression accounts for 50% of transcripts produced by RNA polymerase II49 and their dysregulation leads to reduced fitness47,48. The yeast genome contains 137 RP genes, of which 19 encode a unique RP and 59 are duplicated. The proper functioning of ribosomes requires that all the ribosome components be expressed in equimolar concentrations50 while simultaneously remaining responsive to physiological needs51,52. This is potentially challenging given the copy-number differences between the RP genes because high copy number genes generally show increased expression. The regulatory mechanisms underlying this fine-tuned regulation are not known. By accurately predicting the activity of the RP genes using the promoter sequences, we demonstrate that a considerable amount of this information is encoded in the DNA sequence.

It is intriguing that our model did not explicitly use transcription factor binding site information and focused only on the 100 bp upstream region. Some of the features identified by our model may influence transcription factor binding or nucleosomes indirectly, and could even affect mRNA translation. Transcription factors are critical for gene regulation. Their empirically identified binding sites are 6 to 8 bp, theoretically putting an upper bound on the level of regulatory flexibility that can be attained by mutating positions at these sites30,38. Cooperation between transcription factors or competition among them1517, and with nucleosomes23, provides an additional mechanism for fine-tuned gene expression. RP promoters with high activity have not only more nucleosome disfavoring sequences but also characteristic spatial organization of the binding sites for Rap1, Sfp1 and Fhl130. The low performance of our model on synthetic promoters containing targeted mutations in transcription factor binding sites and nucleosome disfavoring sequences reinforces the importance of these factors. Consistent with this, the combination of our model and the mechanistically driven model involving transcription factors and nucleosome binding30 was more predictive of promoter activity34. Our findings have implications for understanding the fine-tuned regulation of RP genes and engineering desirable activity in synthetic promoters.

Data availability

F1000Research: Dataset 1. Raw data for ‘Prediction of fine-tuned promoter activity from DNA sequence’, Siwo et al. 2016, 10.5256/f1000research.7485.d11351657

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Siwo G, Rider A, Tan A et al. Prediction of fine-tuned promoter activity from DNA sequence [version 1; peer review: 1 approved, 2 approved with reservations]. F1000Research 2016, 5:158 (https://doi.org/10.12688/f1000research.7485.1)
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ApprovedThe paper is scientifically sound in its current form and only minor, if any, improvements are suggested
Approved with reservations A number of small changes, sometimes more significant revisions are required to address specific details and improve the papers academic merit.
Not approvedFundamental flaws in the paper seriously undermine the findings and conclusions
Version 1
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Reviewer Report 20 Jun 2016
Jan Grau, Institute of Computer Science, Martin Luther University of Halle-Wittenberg, Halle, Germany 
Approved with Reservations
VIEWS 30
The authors present FIrST, an approach for predicting promoter activity from sequence, which won one of the DREAM6 challenges. FIrST is using only simple sequence features in a limited range (100 bp) upstream of the translation start site for making ... Continue reading
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Grau J. Reviewer Report For: Prediction of fine-tuned promoter activity from DNA sequence [version 1; peer review: 1 approved, 2 approved with reservations]. F1000Research 2016, 5:158 (https://doi.org/10.5256/f1000research.8064.r14225)
NOTE: it is important to ensure the information in square brackets after the title is included in all citations of this article.
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Reviewer Report 07 Apr 2016
Jianhua Ruan, Department of Computer Science, The University of Texas at San Antonio, San Antonio, TX, USA 
Approved with Reservations
VIEWS 27
This article describes the winning method of the DREAM6 promoter activity prediction challenge. While a meta analysis of the competing methods participated in the challenge has already been published (Meyer et al., 2013), this article provides more details of the ... Continue reading
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Ruan J. Reviewer Report For: Prediction of fine-tuned promoter activity from DNA sequence [version 1; peer review: 1 approved, 2 approved with reservations]. F1000Research 2016, 5:158 (https://doi.org/10.5256/f1000research.8064.r12380)
NOTE: it is important to ensure the information in square brackets after the title is included in all citations of this article.
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Reviewer Report 02 Mar 2016
Paul Pavlidis, Centre for High-Throughput Biology and Department of Psychiatry, University of British Columbia, Vancouver, BC, Canada 
Approved
VIEWS 33
Siwo et al. give a detailed report of their entry to the DREAM promoter activity prediction assessment, conducted in 2011. The paper describing the results of the assessment appeared in 2013 (Meyer et al.), and the entry from Siwo et ... Continue reading
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Pavlidis P. Reviewer Report For: Prediction of fine-tuned promoter activity from DNA sequence [version 1; peer review: 1 approved, 2 approved with reservations]. F1000Research 2016, 5:158 (https://doi.org/10.5256/f1000research.8064.r12530)
NOTE: it is important to ensure the information in square brackets after the title is included in all citations of this article.

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Not approved - fundamental flaws in the paper seriously undermine the findings and conclusions
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