Growth performance and feed utilization of keureling fish <i>Tor tambra</i> (Cyprinidae) fed formulated diet supplemented with enhanced probiotic.

Zainal A. Muchlisin; Tanzil Murda; Cut Yulvizar; Irma Dewiyanti; Nur Fadli; Fardin Afrido; Mohd Nor Siti-Azizah; Abdullah A. Muhammadar

doi:10.12688/f1000research.10693.1

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Research Article

Growth performance and feed utilization of keureling fish Tor tambra (Cyprinidae) fed formulated diet supplemented with enhanced probiotic.

[version 1; peer review: 2 approved]

Zainal A. Muchlisin ¹, Tanzil Murda¹, Cut Yulvizar², [...] Irma Dewiyanti¹, Nur Fadli¹, Fardin Afrido¹, Mohd Nor Siti-Azizah³, Abdullah A. Muhammadar¹

Zainal A. Muchlisin ¹, Tanzil Murda¹, [...] Cut Yulvizar², Irma Dewiyanti¹, Nur Fadli¹, Fardin Afrido¹, Mohd Nor Siti-Azizah³, Abdullah A. Muhammadar¹

PUBLISHED 14 Feb 2017

Author details Author details

¹ Department of Aquaculture, Faculty of Marine and Fisheries, Syiah Kuala University, Banda Aceh, Indonesia
² Department of Biology, Faculty of Natural Science, Syiah Kuala University, Banda Aceh, Indonesia
³ School of Biological Sciences, University Sains Malaysia, Penang, Malaysia

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Abstract

Background
The objective of the present study was to determine the optimum dosage of probiotic in the diet of keureling fish (Tor tambra) fry.
Methods
Lactobacillus casei from Yakult® was used as a starter, and enhanced with Curcuma xanthorrhiza, Kaempferia galanga and molasses. The mixture was fermented for 7 days prior to use as probiotic in a formulated diet containing 30% crude protein. Four levels of probiotic dosage; 0 ml kg^-1 (control), 5 ml kg^-1, 10 ml kg^-1 and 15 ml kg^-1 were tested in this study. The fish was fed twice a day at 08.00 AM and 06.00 PM at the ration of 5% body weight for 80 days.
Results
The results showed that growth performance and feed efficiency increased with increasing probiotic dosage in the diet from control (no probiotic) to 10 ml kg^-1 of probiotic dosage and then decreased when the dosage was increased up to 15 ml kg^-1.
Conclusions
The best values for all measured parameters were recorded at the dosage of 10 ml kg^-1. Therefore, it was concluded that the optimum dosage of enhanced probiotic for T. tambra fry was 10 ml kg^-1 of feed.

Keywords

Mahseer, Probiotic, Curcuma xanthorrhiza, Kaempferia galangal, Lactobacillus casei,

Corresponding author: Zainal A. Muchlisin

Competing interests: We declare no competing interests with PT Yakult Indonesia Persada, the producer of Yakult® in Indonesia.

Grant information: This study was supported by Syiah Kuala University and the Ministry of Research, Technology and Higher Education of the Republic of Indonesia through the Penelitian Unggulan Perguruan Tingi scheme.
The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

Copyright: © 2017 Muchlisin ZA et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The author(s) is/are employees of the US Government and therefore domestic copyright protection in USA does not apply to this work. The work may be protected under the copyright laws of other jurisdictions when used in those jurisdictions. Data associated with the article are available under the terms of the Creative Commons Zero "No rights reserved" data waiver (CC0 1.0 Public domain dedication).

How to cite: Muchlisin ZA, Murda T, Yulvizar C et al. Growth performance and feed utilization of keureling fish Tor tambra (Cyprinidae) fed formulated diet supplemented with enhanced probiotic. [version 1; peer review: 2 approved]. F1000Research 2017, 6:137 (https://doi.org/10.12688/f1000research.10693.1) First published: 14 Feb 2017, 6:137 (https://doi.org/10.12688/f1000research.10693.1) Latest published: 14 Feb 2017, 6:137 (https://doi.org/10.12688/f1000research.10693.1)

Introduction

Aquaculture is a promising business and growing faster in recent years. In Indonesia the most common species of freshwater fish used for aquaculture are several introduced species, for example, tilapia (Oreochromis niloticus), common carp (Cyprinus carpio), and African catfish Clarias gariepinus^1,2. However, Indonesia has a great diversity of freshwater fish species³, several of which have the potential for aquaculture. Muchlisin⁴ evaluated 114 species of freshwater fish from the waters of Aceh Province. In total, 40 species are being utilized for consumption, and 14 species have a high economic value and great potential to be utilized as such. One of these species is Tor tambra, locally known as keureling fish.

Presently, aquaculture of keureling fish has already been initiated in Aceh Province, Indonesia. Several studies on this species have been documented, for example, Muchlisin⁵ has reported the domestication techniques for broodstock, and the prevalence of ectoparasites and endoparasites in keureling fish^6–7. Moreover, Muchlisin et al.⁸ reported that a diet of 30% protein gave the best growth performance for T. tambra fry, compared to 20% and 25% protein. Muchlisin et al.^9–10 have also studied the effect of papain enzyme and additional vitamins in the diet. However, the growth pattern analysis has shown that cultured fish display slower growth compared to wild populations¹¹. This is probably due to low protein digestibility resulting in low feed efficiency. It has been suggested that the growth performance of cultured populations could be enhanced through addition of probiotic to the diet to increase feed efficiency¹². Probiotic in the diet functions as an agent that triggers the metabolism of nutrients from complex compounds into simpler compounds which are readily absorbed by the intestine^13,14. Several studies have reported that addition of probiotic to the diet has a significant effect on growth performance in some species of freshwater fish, for example, catfish Pangasius sutchi and Pangasius hypothalamus^15,16, nile tilapia Oreochromis niloticus¹⁷, Catla catla¹⁸, gourami Osphronemus gourami¹⁹ and three-spot gourami Trichopodus trichopterus²⁰.

It is important to overcome the low growth problem of T. tambra in captivity, and presently there is no study available on the effects of adding probiotic to the T. tambra diet. Therefore, we evaluated the effect of probiotic Lactobacillus casei from Yakult^® enhanced with temulawak (Curcuma xanthorrhiza) and kencur (Kaempferia galanga) on the growth performance and feed utilization of T. tambra.

Methods

Experimental diet

The study was conducted at local aquaculture ponds at Desa Meunasah Krueng, in the Beutong Subdistrict of the Nagan Raya District, from August 2014 to December 2014. The completely randomized design was utilized in this study. Four levels of probiotic dosage were tested, namely: 0 ml probiotic kg^-1 of feed (control), 5 ml probiotic kg^-1 of feed, 10 ml probiotic kg^-1 of feed, and 15 ml probiotic kg^-1 of feed. Every treatment was replicated three times. The experimental diet containing 30% protein was prepared using raw materials purchased from the local market. Each material and formulated feed was tested for crude protein content (Table 1).

Table 1. The raw materials of the formulated diet containing 30% protein used in the study.

Materials	Crude protein content of material (%)	Proportion in the diet (g kg^-1)	Total crude protein in the diet (%)
Fishmeal	45	210	9.45
Soybean meal	42	200	8.40
Corn flours	10	40	0.40
Fine rice-bran	9	280	2.52
Ebi shrimp meal	45	205	9.22
Cassava flours	1.5	15	0.02
Vitamins mix	-	15	-
Minerals mix	-	15	-
Soybean oil	-	20	-

Feeding and handling of keureling fish

A total of 180 T. tambra fish fry with average length of 3.5 cm and average weight of 0.36 g were used in this study. The fry was purchased from the local farmer in the Nagan Raya District and distributed randomly into 12 1m × 1m × 1m hapas (cage settle nets) in a 25m × 20m × 1.3m ground pond at the stocking density of 15 fishes per hapa. The fish were weaned for 7 days prior to experimental procedures. They were fed the experimental diet minus probiotic during the weaning process. After weaning, the fish were fed on experimental diet at a feeding level of 5% body weight twice a day (08.00 AM and 06.00 PM) for 80 days. The pond was equipped with a water flow system at a water discharge of 120 L min^-1. The weight gain was calculated at 10 day intervals. The feces of the fish were collected from respective hapas to examine the protein content of the feces using the Kjeldahl method²¹.

Crude protein and lipid analysis of raw materials and feces

The crude protein in the raw material, feces and experimental diet was measured using the Kjeldahl method²¹. About one gram of dry sample (raw material, experimental diet or feces) was weighed and placed into Kjeldahl beakers, then 10 g of catalyst (SeOCl₂, Selenium Oxydichloride) and 25 ml sulfuric acid were added into the same beaker. The sample was heated to 250 °C for 20 minutes, shaken carefully, then heated to 350 °C for 2 hours. The samples were left to cool for 10 minutes, then 300 ml distilled water was added. Diluted samples were distilled, and this was followed by titration using 0.1 N HCl. Crude lipid was measured by chloroform-methanol extraction²¹. Samples of the raw material or diet were homogenized with a high-speed homogenizer for 5 min and lipid was determined gravimetrically after solvent separation and vacuum drying.

Probiotic preparation

The probiotic was prepared using a mixture of temulawak (C. xanthorrhiza), kencur (K. galanga), molasses and Yakult^® (Lactobacillus casei) as a starter. The Yakult^® was purchased from a local market in Banda Aceh, Indonesia. For 1 liter of probiotic mixture the following is needed: 50 g of temulawak, 100 g kencur, 100 ml of molasses and 1 bottle of Yakult^®. All materials were mashed and mixed, then placed in sealed containers and fermented for 7 days. Every 2 days the container was opened to remove the gas of fermentation. For each experiment, the corresponding amount of probiotic solution was mixed with egg yolk, whisked and sprayed evenly on the diet, then dried at room temperature for 30 minutes prior to feeding to the experimental fish.

Parameters and data analysis

Weight gain (Wg) and specific growth rate (SGR), food conversion ratio (FCR) and feed efficiency (FE) were calculated as follows:

a. Wg = Wt – Wo, where Wg = weight gain (g), Wt = weight at the end of the experiment (g), Wo = weight at start of the experiment (g)
b. The specific growth rate is the percentage of weight gain per day. SGR (% day^-1) = (Ln Wt – Ln Wo) / t x 100, where Ln= Logarithm natural, t = experiment duration (day)²². Daily growth and survival rates were also calculated, based on Muchlisin et al.¹⁰.
c. The Feed Conversion Ratio is the amount of feed (g) required to produce 1 gram of fish²³, FCR = F/(Wt – Wo), where F= the amount of given feed (g)
d. The Feed Efficiency is the total weight gain produced per total weight of feed consumed²³, FE (%)= (1/ FCR) x 100.

All data were subjected to analysis of variance (ANOVA), followed by the comparison of means using Duncan’s multiple range test²⁴.

Statement on animal ethics

All procedures involving animals were conducted in compliance with The Syiah Kuala University Research and Ethics Guidelines, Section of Animal Care and Use in Research (Ethic Code No: 958 /2015). Please refer to Supplementary File 1 for the completed ARRIVE guidelines checklist.

Results

Estimated weight gain the keureling fish fry ranged between 0.73 g to 1.48 g, and the specific growth rate was 1.40% to 2.04%. and the survival rate was 66.67% to 95.56%(Table 2). Feed efficiency ranged between 28.40% to 42.21% and feed conversion ratio ranged between 2.37 to 3.52 (Table 2). ANOVA revealed that probiotic had a significant effect on the growth performance, survival rate, feed efficiency, feed conversion and crude protein content in the feces of keureling fish fry (P<0.05), where the best results were observed at a dosage of 10 ml probiotic kg^-1 of feed. The growth trend of the keureling fish fry during the experiment significantly increased from day 10 to day 40 and from day 50 to day 80 (Figure 1).

Table 2. Average (±SD) growth performance, survival rate, feed efficiency, feed conversion ratio, and protein in the feces of keureling fish fry (Tor tambra) fed for 80 days at different dosage of probiotic.

All data were subjected to analysis of variance (ANOVA), followed by the comparison of means using Duncan’s multiple range test. The means at different probiotic dosage across each parameter were compared. When means have the same superscript (a, b, c, d), they are not significantly different.

Probiotic dosage (ml kg^-1)	Weight gain (g)	Specific growth rate (% day^-)	Feed efficiency (%)	Feed conversion ratio	Crude protein in the feces (%)	Survival rate (%)
0	0.73 ± 0.02^a	1.40 ±0.03^a	28.40 ±0.27^a	3.52 ± 0.33^c	10.06 ± 0.03^c	66.67 ±13.33^a
5	1.07 ± 0.01^b	1.72 ±0.02^b	34.80 ±0.17^b	2.87 ± 0.01^ab	13.89±0.11^b	68.89 ±3.85^a
10	1.48 ± 0.04^d	2.04±0.04^d	42.21 ±0.97^bc	2.37 ± 0.06^a	4.53±0.11^a	95.56 ±3.85^b
15	1.24 ± 0.04^c	1.85 ±0.06^c	35.26 ±0.14^b	2.84 ± 0.01^ab	6.57±0.22^a>	68.89 ±10.12^a

Figure 1. The growth trend of T. tambra fry at different dosage of probiotic during the study.

Discussion

The study revealed that a probiotic dosage of 10 ml kg^-1 gave the best results compared to other dosages. 10 ml kg^-1 may provide an optimum condition for digestive bacteria such as Lactobacillus sp. to grow well and facilitate feed digestibility. This is based on the low protein content in the feces, an indication that the protein was digested better at this dosage. Arief et al.²⁵ stated that Lactobacillus sp. has the ability to balance and enhance microbial condition in the digestive tract by converting carbohydrates into lactic acid, which reduces the pH and so improves the digestibility functions of the tilapia fish, Oreochromis niloticus. This would then stimulate the production of endogenous enzymes to improve absorption of nutrients, and inhibit the growth and activity of pathogenic organisms in the digestive tract. Irianto²⁶ also stated that the addition of probiotics to the diet increases the amount and activity of bacteria in the digestive tract of tilapia fish, and stimulates bacteria to secrete digestive enzymes such as protease and amylase which play an important role in protein and carbohydrates digestion, respectively. Marzouk et al.²⁷ stated that the activities of natural digestive bacteria of tilapia would change significantly when supplemented with external digestive microbes. The activity of probiotic bacteria greatly affects the balance of microflora in the digestive tract that will suppress other pathogenic bacteria resulting in increased digestive efficiency²⁸.

Temulawak and kencur contain bioactive compounds such as curcumins and atsiri oil, respectively, with associated health benefits. These compounds can function as antibiotics, neutralize toxins and increase the secretion of bile²⁹. This improves the digestive system and increases appetite in fish thus accelerating their growth performance. A similar finding by Hassan et al.³⁰ reported that the combination of K. galangal and yeast probiotic had a significant effect on the growth performance and product quality of the Labeo rohita fingerling. Besides, curcumin also helps promote the immune system³¹.

However, excessive probiotics could hamper growth as recorded in this study. As observed the growth performance increased from control (without probiotic) up to 10 ml kg^-1 then decreased when the probiotic dosage was upped to 15 ml kg^-1. According to Atlas and Bartha³², higher doses of probiotics favour production of secondary metabolites due to the increased bacterial load, leading to competition for nutrient and substrate utilization and inhibition of digestion and nutrient absorption. Pelczar and Chan³³ stated that excessive secondary metabolites will kill some bacteria groups, reducing digestibility. Therefore a number of digestive bacteria should be at an optimum level but this differs among species.

Conclusions

Addition of probiotics to the diet of the keureling fish (T. tambra) could enhance growth performance, feed efficiency, feed conversion, protein retention and protein digestibility of larva. We found that 10 ml probiotic kg^-1 of feed was an optimum dosage for this species.

Dataset 1.Raw data and processed data collected for the study.

This includes crude protein in the feces, growth performance, daily growth rate, specific growth rate, survival rate, weight, feed conversion ratio.

Dataset 2.Raw data of Tor tambra weight gain.

Data availability

Dataset 1: Raw data and processed data collected for the study. This includes crude protein in the feces, growth performance, daily growth rate, specific growth rate, survival rate, weight, feed conversion ratio.

DOI, 10.5256/f1000research.10693.d151536³⁴

Dataset 2: Raw data of Tor tambra weight gain.

DOI, 10.5256/f1000research.10693.d151537³⁵

Author contributions

ZAM developed the research proposal and study design and approved the final draft of the paper. TM, TA and ID were responsible for conducting experiments and data collection. CY, AAM and NF carried out data analysis and were involved in report drafting. MNS was responsible for proximate analysis and proofreading of the final draft.

Competing interests

We declare no competing interests with PT Yakult Indonesia Persada, the producer of Yakult^® in Indonesia.

Grant information

This study was supported by Syiah Kuala University and the Ministry of Research, Technology and Higher Education of the Republic of Indonesia through the Penelitian Unggulan Perguruan Tingi scheme.

The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

Acknowledgements

We thank Syiah Kuala University and the Ministry of Research, Technology and Higher Education of the Republic of Indonesia for supporting this study. The authors thank Mr. Khaidir for his assistance during the field experiment in Nagan Raya District.

Supplementary material

Supplementary Figure 1: Completed ARRIVE guidelines checklist.

Click here to access the data.

Faculty Opinions recommended

References

1. Muchlisin ZA: Analisis kebijakan introduksi spesies ikan asing di perairan umum daratan Provinsi Aceh. Journal Kebijakan Sosial Ekonomi Kelautan dan Perikanan. 2011; 1(1): 79–89. Reference Source
2. Muchlisin ZA: First report on introduced freshwater fishes in the waters of Aceh, Indonesia. Arch Pol Fish. 2012; 20(2): 129–135. Publisher Full Text
3. Muchlisin ZA, Siti-Azizah MN: Diversity and distribution of freshwater fishes in Aceh waters, northern Sumatra Indonesia. International Journal of Zoological Research. 2009; 5(2): 62–79. Publisher Full Text
4. Muchlisin ZA: Study on potency of freshwater fishes in Aceh waters as a basis for aquaculture and conservation development programs. Jurnal Iktiologi Indonesia. 2013a; 13(1): 91–96. Reference Source
5. Muchlisin ZA: A preliminary study on the domestication of wild keureling broodfish (Tor tambra). Proceedings of the 3rd Annual International Conference Syiah Kuala University (AIC Unsyiah) 2013 in conjunction with The 2nd International Conference on Multidisciplinary Research (ICMR) 2013 October 2–4, 2013 Banda Aceh, Indonesia. 2013; 380–382. Reference Source
6. Muchlisin ZA, Munazir AM, Fuady Z, et al.: Prevalence of ectoparasites on mahseer fish (Tor tambra. Valenciennes, 1842) from aquaculture ponds and wild population of Nagan Raya District, Indonesia. Human and Veterinary Medicine. 2014; 6: 148–152. Reference Source
7. Muchlisin ZA, Fuadi Z, Munazir AM, et al.: First report on Asian fish tapeworm (Bothriocephalus acheilognathi) infection of indigenous mahseer (Tor tambra) from Nagan Raya District, Aceh Province, Indonesia. Bulgarian Journal of Veterinary Medicine. 2015a; 18: 361–366. Publisher Full Text
8. Muchlisin ZA, Nazir M, Fadli N, et al.: Growth performance, protein and lipid retentions on the carcass of Acehnese mahseer, Tor tambra (Pisces: Cyprinidae) fed commercial diet at different levels of protein. Iranian Journal of Fisheries Science. 2017; Accepted paper.
9. Muchlisin ZA, Afrido F, Murda T, et al.: The effectiveness of experimental diet with varying levels of papain on the growth performance, survival rate and feed utilization of keureling fish (Tor tambra). Biosaintifika. 2016a; 8(2): 172–177. Publisher Full Text
10. Muchlisin ZA, Arisa A, Muhammadar AA, et al.: Growth performance and feed utilization of keureling (Tor tambra) fingerlings fed a formulated diet with different doses of vitamin E (alpha-tocopherol). Arch Pol Fish. 2016b; 23: 47–52. Publisher Full Text
11. Muchlisin ZA, Batubara AS, Siti-Azizah MN, et al.: Feeding habit and length weight relationship of keureling fish, Tor tambra. Valenciennes, 1842 (Cyprinidae) from the western region of Aceh Province, Indonesia. Biodiversitas. 2015a; 16(1): 89–94. Publisher Full Text
12. Bruno GG, Ana R, Turnbull JF: The use and selection of probiotic bacteria for use in the culture of larval aquatic organisms. Aquaculture. 2000; 191(1–3): 259–270. Publisher Full Text
13. Balcázar JL, Blas ID, Ruiz Z, et al.: The role of probiotics in aquaculture. Veterinary Microbiology. 2006; 114(3–4): 173–186. Publisher Full Text
14. Al-Baadani HH, Abudabos AM, Al-Mufarrej SI, et al.: Effects of dietary inclusion of probiotics, prebiotics and synbiotics on intestinal histological changes in challenged broiler chickens. S Afr J Anim Sci. 2016; 46(2): 157–165. Publisher Full Text
15. Andriyanto S, Listyanto N, Rosmawati R: Effect difference doses of probiotic on the survival and growth rates Pangasius djambal. Prosiding Forum Inovasi Teknologi Akuakultur. 2010; 117–122.
16. Setiawati JE, Tarsim Adiputra AY, Hudaidah S: Effect of different doses of probiotic on the diet on the growth performance, survival rate, feed efficiency and protein retention of Pangasius hypophthalmus. Jurnal Rekayasa dan Teknologi Budidaya Perairan. 2013; 1: 151–162.
17. Jatobá A, Vieira Fdo N, Buglione-Neto CC, et al.: Diet supplemented with probiotic for Nile tilapia in polyculture system with marine shrimp. Fish Physiol Biochem. 2011; 37(4): 725– 732. PubMed Abstract | Publisher Full Text
18. Bandyopadhyay P, Das Mohapatra PK: Effect of a probiotic bacterium Bacillus circulans PB7 in the formulated diets: on growth, nutritional quality and immunity of Catla catla (Ham.). Fish Physiol Biochem. 2009; 35(3): 467–478. PubMed Abstract | Publisher Full Text
19. Abdullah IA: Effect of EM-4 probiotic addition on the diet on the growth performance and survival rate of Osphronemus gouramy. Thesis, Universitas Muhammadiyah Malang,. Malang. 2007; 68.
20. Jafaryan H, Sahandi J, Dorbadam JB: Growth and length-weight relationships of Trichopodus trichopterus (Pallas, 1770) fed a supplemented diet with different concentrations of probiotic. Croatian Journal of Fisheries. 2014; 72(3): 118–122. Reference Source
21. Helrich K, Association of Official Analytical Chemistry (AOAC): Official methods of analysis of the association of official analytical chemists. 15th edn (ed. by W. Horwitz). Association of Official Analytical Chemists, Arlington, VA, USA. 1298. Reference Source
22. De Silva SS, Anderson TA: Fish nutrition in aquaculture. Chapman and Hall. London. 1995. Reference Source
23. Card LE, Nesheim MC: Poultry production.11^th Edit. Lea and Febiger. Phildelphia. 1976. Reference Source
24. Sofyan H, Werwatz A: Analyzing XploRe download profiles with intelligent miner. Comput Stat. 2001; 16(3): 465–479. Publisher Full Text
25. Arief M, Kusumaningsih E, Rahardja BS: Crude protein and crude fiber on the formulated diet which fermented with probiotic. Jurnal Ilmiah Perikanan dan Kelautan. 2008; 3(2): 53–58.
26. Irianto A: Probiotic in aquaculture. Universitas Gadjah Mada Press,. Yogjakarta. 2003; 125.
27. Marzouk MS, Moustafa MS, Mohamed N: The influence of some probiotics on the growth performance and intestinal microbial flora of O. niloticus. Proceeding of the 8th International Symposium on Tilapia in Aquaculture. 2008. 1059–1071. Reference Source
28. Verschuere L, Rombaut G, Sorgeloos P, et al.: Probiotic bacteria as biological control agents in aquaculture. Microbiol Mol Biol Rev. 2000; 64(4): 655–671. PubMed Abstract | Publisher Full Text | Free Full Text
29. Araújo CC, Leon LL: Biological activities of Curcuma longa L. Mem Inst Oswaldo Cruz. 2001; 96(5): 723–728. PubMed Abstract | Publisher Full Text
30. Hassan MA, Aftabuddin M, Meena DK, et al.: Effects of black thorn, Kaempferia galanga single or in combination with yeast probiotic on feed palatability, growth performance and product quality of Labeo rohita fingerling (Hamilton). Turk J Fish Aquat Sci. 2014; 14(4): 915–920.
31. Jagetia GC, Aggarwa BB: "Spicing up" of the immune system by curcumin. J Clin Immunol. 2007; 27(1): 19–35. PubMed Abstract | Publisher Full Text
32. Atlas RM, Bartha R: Microbial Ecology: Fundamentals and Applications. Fourth Edition. Addison Wesley Longman, Menlo Park, California. 1998; 576. Reference Source
33. Pelczar MJR, Chan ECS: Microbiology. McGraw-Hill Book Company,. New York. 1997; 952.
34. Muchlisin ZA, Murda T, Yulvizar C, et al.: Dataset 1 in: Growth performance and feed efficiency of keureling fish Tor tambra (Pisces: Cyprinidae) fed formulated diet with enhanced probiotic. F1000Research. 2017. Data Source
35. Muchlisin ZA, Murda T, Yulvizar C, et al.: Dataset 2 in: Growth performance and feed efficiency of keureling fish Tor tambra (Pisces: Cyprinidae) fed formulated diet with enhanced probiotic. F1000Research. 2017. Data Source

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Author details Author details

¹ Department of Aquaculture, Faculty of Marine and Fisheries, Syiah Kuala University, Banda Aceh, Indonesia
² Department of Biology, Faculty of Natural Science, Syiah Kuala University, Banda Aceh, Indonesia
³ School of Biological Sciences, University Sains Malaysia, Penang, Malaysia

Competing interests

We declare no competing interests with PT Yakult Indonesia Persada, the producer of Yakult® in Indonesia.

Grant information

This study was supported by Syiah Kuala University and the Ministry of Research, Technology and Higher Education of the Republic of Indonesia through the Penelitian Unggulan Perguruan Tingi scheme.
The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

Article Versions (1)

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Published: 14 Feb 2017, 6:137

https://doi.org/10.12688/f1000research.10693.1

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© 2017 Muchlisin ZA et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The author(s) is/are employees of the US Government and therefore domestic copyright protection in USA does not apply to this work. The work may be protected under the copyright laws of other jurisdictions when used in those jurisdictions. Data associated with the article are available under the terms of the Creative Commons Zero "No rights reserved" data waiver (CC0 1.0 Public domain dedication).

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Muchlisin ZA, Murda T, Yulvizar C et al. Growth performance and feed utilization of keureling fish Tor tambra (Cyprinidae) fed formulated diet supplemented with enhanced probiotic. [version 1; peer review: 2 approved]. F1000Research 2017, 6:137 (https://doi.org/10.12688/f1000research.10693.1)

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Reviewer Report 13 Mar 2017

Ahmad Jibril Nayaya, Department of Biological Sciences, Abubakar Tafawa Balewa University (ATBU), Bauchi, Nigeria

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https://doi.org/10.5256/f1000research.11529.r20658

The research was developed on the concept of probiotics being microbes that enhance good health and thereby promote optimal utilization of feed. The authors have shown that use of probiotics can improve feed uptake by the fish thereby reducing the ... Continue reading

The research was developed on the concept of probiotics being microbes that enhance good health and thereby promote optimal utilization of feed. The authors have shown that use of probiotics can improve feed uptake by the fish thereby reducing the cost of production especially where the cost of feed in some places is on the high side.

The title is most appropriate for the work and the Abstract gave a concise summary of the research work. Furthermore, the design and methods fit the work done. The analysis correctly explained what has been obtained from the designed work and appropriately reflects the topic studied.

The Conclusion is balanced and has justified the basis of the results. Useful data with adequate information has been provided to warrant replication of the experiment elsewhere.

Overall, the research has made a remarkable contribution by providing the first well documented information on positive advantage of use of probiotics in the culture of T. tambra.

Competing Interests: No competing interests were disclosed.

I confirm that I have read this submission and believe that I have an appropriate level of expertise to confirm that it is of an acceptable scientific standard.

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Reviewer Report 22 Feb 2017

Usman M. Tang, Faculty of Fisheries and Marine Science, Riau University, Pekanbaru, Indonesia

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https://doi.org/10.5256/f1000research.11529.r20452

In my opinion, state of the art of the study has been well presented, and problem statement was also clear. We assume that authors are already familiar with this species indicate by used references. The experimental design was suitable and ... Continue reading

In my opinion, state of the art of the study has been well presented, and problem statement was also clear. We assume that authors are already familiar with this species indicate by used references. The experimental design was suitable and the data were presented very well. The results have been discussed in a good manner and compared to other previous studies.

As known Tor tambra is one of the native species in Indonesia and this species has been cultured. As already mention by the authors that one the main problem in aquaculture is lack of growth rate and low feed efficiency. Therefore, application of probiotic can solve this problem, this proven by the presented data that the feed with probiotic gave higher of growth rate and feed efficiency compared to control. The new innovation of this study is using temulawak (Curcuma xanthorrhiza) and kencur (Kaempferia galanga) to enhanced the probiotic. Therefore, the finding is very useful for practical use and gives a significant contribution to scientific community.

Competing Interests: No competing interests were disclosed.

I confirm that I have read this submission and believe that I have an appropriate level of expertise to confirm that it is of an acceptable scientific standard.

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VERSION 1 PUBLISHED 14 Feb 2017

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Usman M. Tang, Riau University, Pekanbaru, Indonesia
Ahmad Jibril Nayaya, Abubakar Tafawa Balewa University (ATBU), Bauchi, Nigeria

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13 Mar 2017 | for Version 1

Ahmad Jibril Nayaya, Department of Biological Sciences, Abubakar Tafawa Balewa University (ATBU), Bauchi, Nigeria

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Approved

The research was developed on the concept of probiotics being microbes that enhance good health and thereby promote optimal utilization of feed. The authors have shown that use of probiotics can improve feed uptake by the fish thereby reducing the cost of production especially where the cost of feed in some places is on the high side.

The title is most appropriate for the work and the Abstract gave a concise summary of the research work. Furthermore, the design and methods fit the work done. The analysis correctly explained what has been obtained from the designed work and appropriately reflects the topic studied.

The Conclusion is balanced and has justified the basis of the results. Useful data with adequate information has been provided to warrant replication of the experiment elsewhere.

Overall, the research has made a remarkable contribution by providing the first well documented information on positive advantage of use of probiotics in the culture of T. tambra.

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No competing interests were disclosed.

I confirm that I have read this submission and believe that I have an appropriate level of expertise to confirm that it is of an acceptable scientific standard.

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19 Views

22 Feb 2017 | for Version 1

Usman M. Tang, Faculty of Fisheries and Marine Science, Riau University, Pekanbaru, Indonesia

19 Views Cite this report Responses(0)

Approved

In my opinion, state of the art of the study has been well presented, and problem statement was also clear. We assume that authors are already familiar with this species indicate by used references. The experimental design was suitable and the data were presented very well. The results have been discussed in a good manner and compared to other previous studies.

As known Tor tambra is one of the native species in Indonesia and this species has been cultured. As already mention by the authors that one the main problem in aquaculture is lack of growth rate and low feed efficiency. Therefore, application of probiotic can solve this problem, this proven by the presented data that the feed with probiotic gave higher of growth rate and feed efficiency compared to control. The new innovation of this study is using temulawak (Curcuma xanthorrhiza) and kencur (Kaempferia galanga) to enhanced the probiotic. Therefore, the finding is very useful for practical use and gives a significant contribution to scientific community.

Competing Interests

No competing interests were disclosed.

I confirm that I have read this submission and believe that I have an appropriate level of expertise to confirm that it is of an acceptable scientific standard.

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[1] 1. Muchlisin ZA: Analisis kebijakan introduksi spesies ikan asing di perairan umum daratan Provinsi Aceh. Journal Kebijakan Sosial Ekonomi Kelautan dan Perikanan. 2011; 1(1): 79–89. Reference Source

[2] 2. Muchlisin ZA: First report on introduced freshwater fishes in the waters of Aceh, Indonesia. Arch Pol Fish. 2012; 20(2): 129–135. Publisher Full Text

[3] 3. Muchlisin ZA, Siti-Azizah MN: Diversity and distribution of freshwater fishes in Aceh waters, northern Sumatra Indonesia. International Journal of Zoological Research. 2009; 5(2): 62–79. Publisher Full Text

[4] 4. Muchlisin ZA: Study on potency of freshwater fishes in Aceh waters as a basis for aquaculture and conservation development programs. Jurnal Iktiologi Indonesia. 2013a; 13(1): 91–96. Reference Source

[5] 5. Muchlisin ZA: A preliminary study on the domestication of wild keureling broodfish (Tor tambra). Proceedings of the 3rd Annual International Conference Syiah Kuala University (AIC Unsyiah) 2013 in conjunction with The 2nd International Conference on Multidisciplinary Research (ICMR) 2013 October 2–4, 2013 Banda Aceh, Indonesia. 2013; 380–382. Reference Source

[6] 6. Muchlisin ZA, Munazir AM, Fuady Z, et al.: Prevalence of ectoparasites on mahseer fish (Tor tambra. Valenciennes, 1842) from aquaculture ponds and wild population of Nagan Raya District, Indonesia. Human and Veterinary Medicine. 2014; 6: 148–152. Reference Source

[7] 7. Muchlisin ZA, Fuadi Z, Munazir AM, et al.: First report on Asian fish tapeworm (Bothriocephalus acheilognathi) infection of indigenous mahseer (Tor tambra) from Nagan Raya District, Aceh Province, Indonesia. Bulgarian Journal of Veterinary Medicine. 2015a; 18: 361–366. Publisher Full Text

[8] 8. Muchlisin ZA, Nazir M, Fadli N, et al.: Growth performance, protein and lipid retentions on the carcass of Acehnese mahseer, Tor tambra (Pisces: Cyprinidae) fed commercial diet at different levels of protein. Iranian Journal of Fisheries Science. 2017; Accepted paper.

[9] 9. Muchlisin ZA, Afrido F, Murda T, et al.: The effectiveness of experimental diet with varying levels of papain on the growth performance, survival rate and feed utilization of keureling fish (Tor tambra). Biosaintifika. 2016a; 8(2): 172–177. Publisher Full Text

[10] 10. Muchlisin ZA, Arisa A, Muhammadar AA, et al.: Growth performance and feed utilization of keureling (Tor tambra) fingerlings fed a formulated diet with different doses of vitamin E (alpha-tocopherol). Arch Pol Fish. 2016b; 23: 47–52. Publisher Full Text

[11] 11. Muchlisin ZA, Batubara AS, Siti-Azizah MN, et al.: Feeding habit and length weight relationship of keureling fish, Tor tambra. Valenciennes, 1842 (Cyprinidae) from the western region of Aceh Province, Indonesia. Biodiversitas. 2015a; 16(1): 89–94. Publisher Full Text

[12] 12. Bruno GG, Ana R, Turnbull JF: The use and selection of probiotic bacteria for use in the culture of larval aquatic organisms. Aquaculture. 2000; 191(1–3): 259–270. Publisher Full Text

[13] 13. Balcázar JL, Blas ID, Ruiz Z, et al.: The role of probiotics in aquaculture. Veterinary Microbiology. 2006; 114(3–4): 173–186. Publisher Full Text

[14] 14. Al-Baadani HH, Abudabos AM, Al-Mufarrej SI, et al.: Effects of dietary inclusion of probiotics, prebiotics and synbiotics on intestinal histological changes in challenged broiler chickens. S Afr J Anim Sci. 2016; 46(2): 157–165. Publisher Full Text

[15] 15. Andriyanto S, Listyanto N, Rosmawati R: Effect difference doses of probiotic on the survival and growth rates Pangasius djambal. Prosiding Forum Inovasi Teknologi Akuakultur. 2010; 117–122.

[16] 16. Setiawati JE, Tarsim Adiputra AY, Hudaidah S: Effect of different doses of probiotic on the diet on the growth performance, survival rate, feed efficiency and protein retention of Pangasius hypophthalmus. Jurnal Rekayasa dan Teknologi Budidaya Perairan. 2013; 1: 151–162.

[17] 17. Jatobá A, Vieira Fdo N, Buglione-Neto CC, et al.: Diet supplemented with probiotic for Nile tilapia in polyculture system with marine shrimp. Fish Physiol Biochem. 2011; 37(4): 725– 732. PubMed Abstract | Publisher Full Text

[18] 18. Bandyopadhyay P, Das Mohapatra PK: Effect of a probiotic bacterium Bacillus circulans PB7 in the formulated diets: on growth, nutritional quality and immunity of Catla catla (Ham.). Fish Physiol Biochem. 2009; 35(3): 467–478. PubMed Abstract | Publisher Full Text

[19] 19. Abdullah IA: Effect of EM-4 probiotic addition on the diet on the growth performance and survival rate of Osphronemus gouramy. Thesis, Universitas Muhammadiyah Malang,. Malang. 2007; 68.

[20] 20. Jafaryan H, Sahandi J, Dorbadam JB: Growth and length-weight relationships of Trichopodus trichopterus (Pallas, 1770) fed a supplemented diet with different concentrations of probiotic. Croatian Journal of Fisheries. 2014; 72(3): 118–122. Reference Source

[21] 21. Helrich K, Association of Official Analytical Chemistry (AOAC): Official methods of analysis of the association of official analytical chemists. 15th edn (ed. by W. Horwitz). Association of Official Analytical Chemists, Arlington, VA, USA. 1298. Reference Source

[22] 22. De Silva SS, Anderson TA: Fish nutrition in aquaculture. Chapman and Hall. London. 1995. Reference Source

[23] 23. Card LE, Nesheim MC: Poultry production.11^th Edit. Lea and Febiger. Phildelphia. 1976. Reference Source

[24] 24. Sofyan H, Werwatz A: Analyzing XploRe download profiles with intelligent miner. Comput Stat. 2001; 16(3): 465–479. Publisher Full Text

[25] 25. Arief M, Kusumaningsih E, Rahardja BS: Crude protein and crude fiber on the formulated diet which fermented with probiotic. Jurnal Ilmiah Perikanan dan Kelautan. 2008; 3(2): 53–58.

[26] 26. Irianto A: Probiotic in aquaculture. Universitas Gadjah Mada Press,. Yogjakarta. 2003; 125.

[27] 27. Marzouk MS, Moustafa MS, Mohamed N: The influence of some probiotics on the growth performance and intestinal microbial flora of O. niloticus. Proceeding of the 8th International Symposium on Tilapia in Aquaculture. 2008. 1059–1071. Reference Source

[28] 28. Verschuere L, Rombaut G, Sorgeloos P, et al.: Probiotic bacteria as biological control agents in aquaculture. Microbiol Mol Biol Rev. 2000; 64(4): 655–671. PubMed Abstract | Publisher Full Text | Free Full Text

[29] 29. Araújo CC, Leon LL: Biological activities of Curcuma longa L. Mem Inst Oswaldo Cruz. 2001; 96(5): 723–728. PubMed Abstract | Publisher Full Text

[30] 30. Hassan MA, Aftabuddin M, Meena DK, et al.: Effects of black thorn, Kaempferia galanga single or in combination with yeast probiotic on feed palatability, growth performance and product quality of Labeo rohita fingerling (Hamilton). Turk J Fish Aquat Sci. 2014; 14(4): 915–920.

[31] 31. Jagetia GC, Aggarwa BB: "Spicing up" of the immune system by curcumin. J Clin Immunol. 2007; 27(1): 19–35. PubMed Abstract | Publisher Full Text

[32] 32. Atlas RM, Bartha R: Microbial Ecology: Fundamentals and Applications. Fourth Edition. Addison Wesley Longman, Menlo Park, California. 1998; 576. Reference Source

[33] 33. Pelczar MJR, Chan ECS: Microbiology. McGraw-Hill Book Company,. New York. 1997; 952.

[34] 34. Muchlisin ZA, Murda T, Yulvizar C, et al.: Dataset 1 in: Growth performance and feed efficiency of keureling fish Tor tambra (Pisces: Cyprinidae) fed formulated diet with enhanced probiotic. F1000Research. 2017. Data Source

[35] 35. Muchlisin ZA, Murda T, Yulvizar C, et al.: Dataset 2 in: Growth performance and feed efficiency of keureling fish Tor tambra (Pisces: Cyprinidae) fed formulated diet with enhanced probiotic. F1000Research. 2017. Data Source

Growth performance and feed utilization of keureling fish Tor tambra (Cyprinidae) fed formulated diet supplemented with enhanced probiotic.

Abstract

Keywords

Introduction

Methods

Experimental diet

Table 1. The raw materials of the formulated diet containing 30% protein used in the study.

Feeding and handling of keureling fish

Crude protein and lipid analysis of raw materials and feces

Probiotic preparation

Parameters and data analysis

Statement on animal ethics

Results

Table 2. Average (±SD) growth performance, survival rate, feed efficiency, feed conversion ratio, and protein in the feces of keureling fish fry (Tor tambra) fed for 80 days at different dosage of probiotic.

Figure 1. The growth trend of T. tambra fry at different dosage of probiotic during the study.

Discussion

Conclusions

Data availability

Author contributions

Competing interests

Grant information

Acknowledgements

Supplementary material

References

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