ALL Metrics
-
Views
-
Downloads
Get PDF
Get XML
Cite
Export
Track
Case Report

Case Report: A rare case of BCGitis in a patient with bladder cancer treated with the BCG vaccine

[version 1; peer review: 1 approved, 1 approved with reservations]
PUBLISHED 09 Aug 2019
Author details Author details
OPEN PEER REVIEW
REVIEWER STATUS

Abstract

The Bacillus Calmette Guérin (BCG) vaccine was initially produced as a vaccine against tuberculosis. BCG is an attenuated live strain of Mycobacterium bovis and has been widely used as an immunotherapy over the last years in bladder cancer. We describe a case of a 61-year-old Caucasian male with previous bladder cancer, who had been treated for the last 15 months with instillation of BCG, admitted with 3-week evolution history of fever (38ºC), asthenia, anorexia and a weight loss of 6kg. The patient’s condition deteriorated leading to sepsis. A liver biopsy was performed showing granulomatous dispersed through all the parenchyma. Empirical therapy for M. bovis was started with good response.
Even though it is rare, BCGitis must be ruled out in any patient submitted to immunotherapy with BCG and prompt therapy started if disseminated disease is present, which improves the outcome.

Keywords

BCGitis, Mycobacterium bovis, Bladder cancer, sepsis

Introduction

Most bladder carcinoma are located superficially, described as a non-muscle invasive bladder cancer, which allows a therapeutic approach with local immunotherapy1. The Bacillus Calmette Guérin (BCG) vaccine was initially produced as a vaccine against tuberculosis. BCG is an attenuated live strain of Mycobacterium bovis and has been widely used as an immunotherapy over the last few years2. Treatment with the BCG vaccine is well tolerated by over 95% of patients3.

The most frequent side effects of immunotherapy with the BCG vaccine are limited to the bladder – bacterial or chemical cystitis and haematuria. Although rare, systemic side effects can be mild or severe and they range from fever and influenza-like symptoms to BCG-induced lung infection, liver toxicity and BCG sepsis. Sepsis was estimated to affect 0.3% of patients treated with BCG instillation3. When a disseminated infection is present it can be described as BCGitis, with the lung and liver being the most frequently affected organs (50% and 61%, respectively)4

A diagnosis of BCGitis can be validated by direct staining (Ziehl-Neelsen or auramine-rhodamine), histological observation (presence of a granulomatous pattern with or without caseous necrosis) or the results of polymerase-chain reaction (PCR) assays. However, the rentability of all options is low, leading to increasing difficultly in establishing the diagnosis4,5.

We hereby report a rare case of septic BCGitis.

Case report

A 61-year-old Caucasian male with a medical history of pulmonary embolism and bladder cancer (pTaG1), who had been treated with the BCG vaccine for the last 15-months (a total of 15 instillations) was admitted with a 3-week history of fever (38°C), asthenia, anorexia and a weight loss of 6kg.

A week prior to admission (virgula) the patient presented with dysuria and pollakiuria, justifying an empirical treatment for a urinary tract infection with ciprofloxacin (500mg bid) but without response. the urine culture requested came back negative.

The patient’s general state was aggravated with persistent fever, tachycardia and increased inflammatory markers (CRP 46mg; normal range, <5mg/L), elevated liver enzymes [AST (46U/l); ALT (64U/I)], leading to hospital admission with a diagnosis of sepsis (Day 1; Table 1). An empirical broad-spectrum course of antibiotics with ceftriaxone (2gr) and gentamycin (500mg) was instituted for 5 days. A septic screen was performed, which grew no microorganisms.

Table 1. Evolution of laboratory results from hospital admission to discharge.

Haemoglobin (Hb) is expressed in grams per liter; Neutrophil cells, platelets, lymphocytes and monocytes are expressed as elements /mm³; Aspartate transaminase (AST), alanine transaminase (ALT), gamma-glutamyl transferase (gGT), and alkaline phosphatase (ALP) are all expressed in international units per liter; Total bilirubin is expressed in milligrams per decilitre and C-reactive protein is expressed in milligrams per litre.

Day 1 - admissionD3D7D15D26 - discharged
Hb148147153149139
Leucocytes70007000830097004300
Platelets235.000193000167000279000217
Creatinine1.11.01.00.80.8
AST4654503723
ALT6463635627
gGT-80--62
ALP-72--78
Total bilirrubin-1.4---
INR-1.4--1.2
CRP457618974

After 3 days of hospital stay, the patient’s status continued to deteriorate with persistent fever, hypotension and an increase in CRP to 76mg/L (Day 3; Table 1). No abnormal lymph nodes were observed. An abdominal ultrasound revealed a hyperechoic liver lesion with preservation of length and liver contour (Figure 1). This evidence (virgula) combined with the knowledge that there had been a previous recent administration of immunotherapy with BCG 3 weeks before the onset of the presented clinical symptoms, lead physicians to perform a liver biopsy.

20b59ee6-1082-440d-89e0-5542e7890f6b_figure1.gif

Figure 1. Abdominal ultrasound with posterior echo attenuation and hyperechogenic liver parenchyma characteristic of liver steatosis.

A liver biopsy was conducted revealing multiple small epithelial granulomas without caseous necrosis compatible with granulomatous hepatitis. Ziehl-Neelsen staining and Löwenstein–Jensen medium culture were negative. A PCR assay for M. tuberculosis complex (MTB) was also negative both in the liver biopsy and urine sample.

Due to continuous clinical worsening of the patient’s condition until day 7 and aggravated sepsis with persistent fever, hypotension and increased CRP to 186mg/dL under broad-spectrum antibiotics, an empirical regimen with isoniazid 300mg, rifabutin 300mg and ethambutol 1200mg was started for 3 months. Given its interaction with rivaroxaban, rifampicin was switched to rifabutin. In a matter of days, the clinical outcome with resolution of the fever and normalized the inflammation markers at day seven from admission (Table 1). Moreover, within one week of therapy, CRP, AST and ALT revealed a notorious decrease, with a complete normalization within 11 days upon treatment institution.

The patient´s therapeutic regimen included isoniazid 300mg, rifabutin 300mg and ethambutol 1200mg for the first 3 months and the subsequent 3 months with isoniazid and rifabutin with good adherence and physiological tolerance. After 2 years of treatment, the patient remains in remission.

Discussion

Our case highlights the rare but dangerous systemic secondary side effects of BCG instillations that characterize (singular) BCGitis. Evolution to a multisystemic disease deserves attention because its diagnosis can be a challenge to any physician – unspecified symptoms could also be present in acute infection, autoimmune disorders, evolution of malignancy or drug toxicity.

The pathophysiology of BCGitis is not yet well understood. Previous studies have suggested that it is the result from hypersensitivity, with a diffuse granulomatous reaction without isolation of M. bovis, while others consider it a disseminated disease of M. bovis4.

The identification of Mycobacterium requires a combination of tests because there is a poor reliability of PCR for MTB, Ziehl-Neelsen or auramine-rhodamine stains and also with Löwenstein–Jensen medium culture4,6. The histological finding of granulomatous lesions remains the main support to the diagnosis when it is not possible to identify Mycobacteria, as we describe in this case. However, the steatotic liver pattern presented in Figure 1 supports the presence of liver inflammation and the need of a liver biopsy.

In sensitive strains, the use of empirical antibiotherapy with fluoroquinolone could decrease the accuracy of Ziehl-Neelsen stain, Löwenstein–Jensen medium culture and PCR for MTB.

Options for treatment regimens and duration of therapy also remain under discussion; however, treatment options that have been accepted vary from 6–9 months4,5. Our patient was treated for 6 months, which proved to be adequate for treatment of BCGitis. The interaction of rivaroxaban with rifampicin forced a switch to rifabutin.

Corticosteroids should only be used in severe cases, due to the lack of evidence in mild and moderate presentations4.

Finally, the present case confirms the good clinical and laboratorial response upon institution of empirical therapy, with complete recovery. Moreover, two years after BCGitis, the patient is stable but remains under surveillance.

Conclusions

We describe in detail a very rare case of sepsis secondary to immunotherapy with BCG for a non-muscle invasive bladder cancer. Most frequently, patients have local manifestations, however systemic infection may also occur. The diagnosis can be challenging as other infections and malignancies must be first ruled out. Nonetheless, physicians ought to be familiar with bcgitis and upon diagnosis, prompt therapy must be started.

Consent

Written informed consent for the publication of this case report and any associated images was obtained from the patient.

Data availability

All data underlying the results are available as part of the article and no additional source data are required.

Comments on this article Comments (0)

Version 1
VERSION 1 PUBLISHED 09 Aug 2019
Comment
Author details Author details
Competing interests
Grant information
Copyright
Download
 
Export To
metrics
Views Downloads
F1000Research - -
PubMed Central
Data from PMC are received and updated monthly.
- -
Citations
CITE
how to cite this article
Sousa D, Antunes Silva S, Jorge C et al. Case Report: A rare case of BCGitis in a patient with bladder cancer treated with the BCG vaccine [version 1; peer review: 1 approved, 1 approved with reservations]. F1000Research 2019, 8:1399 (https://doi.org/10.12688/f1000research.20055.1)
NOTE: If applicable, it is important to ensure the information in square brackets after the title is included in all citations of this article.
track
receive updates on this article
Track an article to receive email alerts on any updates to this article.

Open Peer Review

Current Reviewer Status: ?
Key to Reviewer Statuses VIEW
ApprovedThe paper is scientifically sound in its current form and only minor, if any, improvements are suggested
Approved with reservations A number of small changes, sometimes more significant revisions are required to address specific details and improve the papers academic merit.
Not approvedFundamental flaws in the paper seriously undermine the findings and conclusions
Version 1
VERSION 1
PUBLISHED 09 Aug 2019
Views
4
Cite
Reviewer Report 24 Feb 2020
Marco Antonio Yamazaki-Nakashimada, Clinical Immunology Department, Instituto Nacional de Pediatría, Mexico City, Mexico 
Approved with Reservations
VIEWS 4
The authors report a patient presenting an uncommon complication of BCG immunotherapy to treat bladder cancer. It is a very interesting case, with the peculiarity that the patient presented important radiological and histopathological liver involvement without jaundice or very elevated ... Continue reading
CITE
CITE
HOW TO CITE THIS REPORT
Yamazaki-Nakashimada MA. Reviewer Report For: Case Report: A rare case of BCGitis in a patient with bladder cancer treated with the BCG vaccine [version 1; peer review: 1 approved, 1 approved with reservations]. F1000Research 2019, 8:1399 (https://doi.org/10.5256/f1000research.22020.r59867)
NOTE: it is important to ensure the information in square brackets after the title is included in all citations of this article.
Views
6
Cite
Reviewer Report 20 Dec 2019
André Lencastre, Department of Dermatology and Venereology, Centro Hospitalar Universitário de Lisboa Central, Lisbon, Portugal 
Approved
VIEWS 6
The authors report on a case of iatrogenic sepsis induced by BCG immunotherapy to treat bladder cancer. The final diagnosis was made with clinical history, ultrasound imagery and histology of the liver revealing a granulomatous hepatitis. No confirmation was possible testing ... Continue reading
CITE
CITE
HOW TO CITE THIS REPORT
Lencastre A. Reviewer Report For: Case Report: A rare case of BCGitis in a patient with bladder cancer treated with the BCG vaccine [version 1; peer review: 1 approved, 1 approved with reservations]. F1000Research 2019, 8:1399 (https://doi.org/10.5256/f1000research.22020.r57465)
NOTE: it is important to ensure the information in square brackets after the title is included in all citations of this article.

Comments on this article Comments (0)

Version 1
VERSION 1 PUBLISHED 09 Aug 2019
Comment
Alongside their report, reviewers assign a status to the article:
Approved - the paper is scientifically sound in its current form and only minor, if any, improvements are suggested
Approved with reservations - A number of small changes, sometimes more significant revisions are required to address specific details and improve the papers academic merit.
Not approved - fundamental flaws in the paper seriously undermine the findings and conclusions
Sign In
If you've forgotten your password, please enter your email address below and we'll send you instructions on how to reset your password.

The email address should be the one you originally registered with F1000.

Email address not valid, please try again

You registered with F1000 via Google, so we cannot reset your password.

To sign in, please click here.

If you still need help with your Google account password, please click here.

You registered with F1000 via Facebook, so we cannot reset your password.

To sign in, please click here.

If you still need help with your Facebook account password, please click here.

Code not correct, please try again
Email us for further assistance.
Server error, please try again.