ALL Metrics
-
Views
-
Downloads
Get PDF
Get XML
Cite
Export
Track
Review

Recent discoveries and advancements in research on the Lyme disease spirochete Borrelia burgdorferi

[version 1; peer review: 5 approved]
PUBLISHED 31 May 2019
Author details Author details
OPEN PEER REVIEW
REVIEWER STATUS

Abstract

This review highlights some of the highest-profile developments and advancements in the research on Borrelia burgdorferi, the Lyme disease spirochete, that have emerged in the last two years. Particular emphasis is placed on the controversy surrounding genus nomenclature, antigenic variation at the vlsE locus, genes involved in infectivity and virulence, membrane characteristics of B. burgdorferi, and developments in experimental approaches.

Keywords

Borrelia, B. burgdorferi, Lyme disease

Introduction

Borrelia burgdorferi is an intriguing and unique bacterium. It is most notorious for being the primary causative agent of Lyme disease in North America1. With more than 42,000 probable and confirmed cases reported to the Centers for Disease Control and Prevention (CDC) in 2017 and other estimates in the range of 300,000 cases annually in the US, Lyme disease is the most common arthropod-borne disease in North America and Europe2,3 (CDC, https://www.cdc.gov/lyme/datasurveillance/index.html). Case numbers have increased over the past several years and continue to be a growing concern as the range of the tick vector, Ixodes scapularis in particular, expands in North America4 (CDC, https://www.cdc.gov/lyme/datasurveillance/index.html). Although antibiotic regimens are available and effective for treating the early stages of infection, Lyme disease often progresses without diagnosis5. Serious long-term effects include arthritis, carditis, and neuroborreliosis5,6. Consequently, much research has focused on the development of a vaccine to administer to humans in endemic regions such as the Northeast and upper Midwest of the US7.

B. burgdorferi is a motile, Gram-negative, pathogenic spirochete with a highly segmented genome composed of a linear chromosome and about 20 linear and circular genomic plasmids1,8. It exists in an enzootic life cycle alternating between specific Ixodes ticks and vertebrates9. Humans can become incidental hosts through the bite of an infected tick9. Whereas some Borrelia species, including B. burgdorferi, cause Lyme disease, other species cause relapsing fever, which is characterized by recurring episodes of fever and illness correlated with the bacterial burden in the bloodstream10.

The focus of this article is to briefly highlight some of the major developments and advancements in understanding B. burgdorferi published within the last two years. As such, the content of this review is inherently selected on the basis of the authors’ interests and perspectives. Nevertheless, this article provides a summary of some of the most significant recent developments regarding genus nomenclature, antigenic variation at the vls locus, genes involved in infectivity and virulence, membrane chemistry, and advancements in laboratory techniques.

Genus name and species

A recent source of controversy and confusion in the spirochete world surrounds the genus name itself. Historically, B. burgdorferi sensu lato is a designation inclusive of all Lyme disease Borrelia species and includes Borrelia afzelii and Borrelia garinii species known to cause disease in Europe and Asia, whereas B. burgdorferi sensu stricto exclusively denotes B. burgdorferi9. Lyme disease–causing and relapsing fever–causing spirochetes collectively resided in the single genus Borrelia since both clades have general microbiological features in common and genomic similarities (reviewed in 11). The clades do have differences regarding tick vector species, geographic distribution, ecology, transmission, and clinical manifestation and pathogenesis (reviewed in 11). In 2014, Adeolu and Gupta published a controversial proposition to divide the genus into Borrelia and Borreliella, the former including agents of relapsing fever and the latter denoting the Lyme disease–causing species, on the basis of average nucleotide identity (ANI)12. This work aimed to identify conserved signature insertions/deletions and conserved signature proteins that could be used to distinguish between the groups. Their approach was criticized for presupposing differences between the clades in their analysis and overlooking the similarities between these groups, and the proposed naming system has been controversial and largely not adopted in the literature. In recent response to this work, Margos et al. outlined how ANI, though useful for determining relatedness between strains of a given species, is not appropriate for differentiating genera13. Instead, they used pairwise analysis of percentage of conserved proteins (POCP) to determine whether there were differences significant enough to warrant the division of the Borrelia genus13. The result was negative and rather suggested that Borrelia species exist more as a continuum13. In an additional letter to the editor, Margos et al. itemized scientific criticisms and practical concerns about the genus split14, which are largely and rather convincingly countered by Barbour et al.15. A non-scientific yet compelling argument against the genus split is the confusion and public health concern associated with altering the name of a pathogen16. Lending further backing to the reunification of the genus, a phyloproteomic analysis found insufficient functional differences between Lyme disease and relapsing fever clades to warrant individual genera17. Annotated proteomes from species representing each of the five spirochete genera and 40,000 proteins representing key biological processes were compared by a series of methods to demonstrate more relatedness between Lyme disease–causing and relapsing fever–causing Borrelia than exists between species of other genera17. Of additional note, the genome of Borrelia anserina, an avian-associated species, was recently fully sequenced18 and new species continue to be discovered, such as Borrelia mayonii, which was isolated in the North Central US in 201619,20 and echidna-associated Candidatus Borrelia tachyglossi13,21. It is our opinion that the Borrelia genus should not be split into Borrelia and Borreliella, because if different scientific approaches do not yield the same results, no firm conclusion can be drawn at this time. Perhaps as more species are discovered and characterized, the scientific merit or detriment of splitting the genus will become clearer.

Antigenic variation of VlsE

B. burgdorferi is able to establish persistent infection in immunocompetent hosts (as reviewed in 6,9). Antigenic variation in the vlsE locus is thought to play a large part in immune evasion since VlsE is expressed during mammalian infection but not within the tick vector22, and Borrelia strains lacking vlsE are unable to maintain infection in immunocompetent mice as opposed to severe combined immune deficiency (SCID) mice2325. The vls system, encoded on lp28-1 (in B31-type strains), includes a series of silent cassettes upstream of the vlsE promoter26,27. Through a segmental gene conversion mechanism, unidirectional transfers of sequences from the silent cassettes to the vlsE variable regions generate chimeric antigens during infection28. Research on the vlsE locus-switching mechanism has been impaired by the necessity for mammalian infection, coupled with the limitations of sequencing strategies that align short reads, and therefore cannot capture the nuances in genomic changes during infection26,29. In a groundbreaking study, Verhey et al. advanced the field by applying PacBio long-read sequencing to sequence the vls locus26. These long reads are sensitive to single-nucleotide polymorphisms and reduce the ambiguity associated with contig assembly26. These authors also built Variable Antigen Sequence Tracer (VAST), a powerful program that analyzes the data and aligns the vls locus to the reference sequence. This technique was applied to study vlsE switching in the context of infection by injecting wild-type (WT) and SCID mice with B. burgdorferi, harvesting multiple tissues at various time points, and sequencing the re-isolated spirochetes26. A number of interesting findings resulted and build off previous findings30. First, the base-change frequencies of vlsE were dramatically higher in the WT mice in comparison to SCID mice, confirming earlier work suggesting that pressure from the acquired immune response facilitates detection of vlsE variants2325. Second, they observed that 99.6% of vlsE variants were found to be unique in each tissue in any given mouse26. This sheds light on the dissemination process in mice. During infection, B. burgdorferi replicates and disseminates through the bloodstream to different tissues31. The uniqueness of the vlsE variants in each tissue suggests that B. burgdorferi cells colonize a tissue, and reside there, rather than re-entering the bloodstream and colonizing additional tissues26. Third, lack of obvious preference toward switching at the 5′ or 3′ end of the gene suggests that vlsE switching occurs stochastically rather than in a biased fashion26. Fourth, three-dimensional structure mapping of the VlsE amino acid changes found in WT mice versus SCID mice showed that infection in WT mice required switching on surface-exposed loops as opposed to switching in the hydrophobic α-helices26. These hydrophobic α-helices are putatively involved in dimerization, suggesting that functional dimerization of VlsE is necessary in vivo and therefore conserved26. This work was performed with B. burgdorferi strain B31, and similar results were obtained with strain JD1, which differs in several respects: it lacks 17 base-pair direct repeats in the vls system, its silent cassettes are arranged differently, the inverted repeat sequences at the vls locus are different, and the vls sequences themselves are different32. Investigations into other features of the vls locus, such as the long inverted repeat that resides in the vlsE promoter, have been undertaken through the construction of mini-vls plasmids33. This work suggests that vlsE switching occurs in cis and that the inverted repeat is not required for switching to occur33. The mechanistic details whereby vls switching occurs and how VlsE protects B. burgdorferi from the host antibody response remain of great interest for further investigation.

Infectivity and virulence attributes distinct from antigenic variation

The genome of B. burgdorferi is relatively small and does not encode enzymes key to several metabolic processes8. This leaves B. burgdorferi dependent on its host for many nutrients and presumably renders essential many of the genes it does possess. Genes essential for survival in the tick vector or in the mammalian host (or both) continue to be identified. One such gene is bb0210, which encodes Lmp1, an antigenic outer-membrane protein34. Lmp1 appears to be a multifunctional protein (similar to BBK323537) with roles that include adhesion at the middle domain38,39, immune evasion for persistent infection40, and tissue tropism during infection41. Although three domains of Lmp1 were previously recognized41, the necessity of the individual domains for a mammalian infection has not been fully investigated. In a recent publication, Zhuang et al.34 provide evidence for reduced bacterial burden of the Δlmp1 mutant in mouse tissues at 3 weeks post-infection, the requirement for both the N-terminal and middle domains in tick-to-mouse transmission, the existence of multiple Lmp1 species, and the degradation of Lmp1 by the periplasmic serine protease BbHtrA34. Further investigation of the complexities of the in vivo roles of the Lmp1 protein and its fragments is warranted.

In a separate inquiry, recent work demonstrated that BB0405 is necessary for mouse infection42. This is interesting because BB0405 and BB0406 may be pore-forming proteins43 that are immunogenic, highly paralogous (differing by a five–amino acid deletion in the N-terminal region of BB0405 and a nine–amino acid deletion in the C-terminal region of BB0406), and co-transcribed8,42. A null mutant of bb0405 effectively disrupted production of both BB0405 and BB0406 at the protein level. The bb0405 mutant was complemented with either bb0405 or bb0406 by insertion into a different replicon, circular plasmid 26 (cp26). When these strains were used to infect mice, culture-positive tissue samples were recovered for the bb0405-complemented, but not for the bb0405 mutant or bb0406-complemented, strains. This confirms that BB0405 is essential in mammalian infection but the role of BB0406 remains unknown. Despite their similarities, BB0405 and BB0406 do not appear to be co-expressed, as evidenced by reactivity with baboon sera collected at different times post-infection. The mechanism underlying this apparent co-transcription without co-expression of these genes warrants further investigation of the transcriptional and translational regulators critical to the infection process.

Membrane characteristics

The membrane composition and characteristics of B. burgdorferi differ markedly from those of other Gram-negative bacteria. For example, the outer and inner membranes are separated by periplasm and peptidoglycan, as is usual, but also by periplasmic flagella44. Borrelia species lack lipopolysaccharide, and B. burgdorferi has an unusually large number of lipoproteins on its outer membrane45,46. Recent work has investigated the existence of lipid rafts in both the outer and inner membranes. Although this work was performed on outer- and inner-membrane fractions prepared in vitro, data from liquid chromatography, anisotropy and fluorescence resonance energy transfer (FRET) analysis, mass spectrometry, and proton nuclear magnetic resonance (1H-NMR) experiments have led to some interesting conclusions47. Lipid rafts have previously been demonstrated in the outer membrane, but there is now evidence for lipid rafts in the inner membrane48. The inner membrane has a different quantity of cholesterol glycolipids and more unsaturated bonds in the acyl chains of the phospholipids than the outer membrane, causing it to have a lower propensity to lipid raft formation than the outer membrane47. As expected, the proteomic profiles of the membranes differ such that the inner membrane harbors more transport proteins and signaling proteins whereas the outer membrane harbors porins and lipoproteins, at least some of which interact with the host47. In another ambitious study, the localization of the entire lipoproteome of B. burgdorferi was determined49. This was accomplished by using an algorithm to identify putative lipoproteins in the B. burgdorferi genome, then generating a 125-member plasmid library encoding C-terminally His-tagged lipoproteins, and transforming these constructs into B. burgdorferi for analysis49. Transformants were screened for tagged lipoprotein localization by proteinase K digestion, membrane fractionation, pronase digestion, and multidimensional protein identification technology (MudPIT) mass spectrometry49. Although there are always caveats to the use of overexpressed tagged proteins, some of the results are still striking. Of the 125 lipoproteins examined, 86 were surface-exposed, 31 were in the inner membrane and exposed to the periplasm, and only eight were in the outer membrane and exposed to the periplasm49. This is in contrast to other Gram-negative bacteria, which have higher proportions of lipoproteins in the outer membrane exposed to the periplasm, again highlighting the uniqueness of B. burgdorferi49. Although the significance of differential lipoprotein expression and localization during tick and mammalian infection is one of the most established tenets in Borrelia biology, a comprehensive study had never been performed. Understanding the membranes of B. burgdorferi and their components may well lead to identification of novel vaccine targets in the future.

Progress in the development of experimental approaches

Genetic manipulation of B. burgdorferi has been greatly hindered by a number of factors, including slow growth, loss of genomic plasmids during in vitro cultivation, lack of a minimal defined medium, a highly A/T-rich genome, and lack of homology in many cases to the genes and proteins of model organisms8,50,51. Therefore, many of the tools and techniques used for the manipulation of bacteria such as Escherichia coli have not been tractable in B. burgdorferi. However, great strides have recently been made to expand the toolbox. In 2016, the first report of the application of transposon sequencing (Tn-seq) in the context of B. burgdorferi infection was published52. This study used the signature-tagged Mariner Himar1 transposon library in an infectious strain background to address the utilization of various carbohydrate sources in vitro and to identify genes important during mammalian infection5255. The following year, Tn-seq was used to identify genes involved in resistance to nitric oxide, hydrogen peroxide, and tert-butyl hydroperoxide in vitro56. These studies pave the way for future high-throughput screens both in vitro and in vivo for B. burgdorferi.

An additional advance in B. burgdorferi research is in the introduction of constitutive promoters of different strengths. Although strong promoters from the flaB and flgB genes have been used historically, transcriptome data have now been used to identify constitutively weak (P0526) and moderate (P0826, PresT, P0031, and P0026) promoters which will be useful for expressing more biologically relevant levels of a gene of interest57. Furthermore, monomeric fluorescent markers have been adapted for B. burgdorferi, including cyan, green, yellow, red, and infrared fluorescent proteins. The infrared marker requires the addition of exogenous biliverdin, but the excitation wavelength is less toxic to cells than other markers and results in less autofluorescence. These markers will inevitably prove useful in co-localization studies and other research and allow simultaneous imaging of up to four distinct proteins in a cell57. In the same publication, the options for antibiotic markers were expanded. Historically, kanamycin (aphI), gentamicin (aacC1), and streptomycin (aadA) resistance genes have been the available markers of choice57. Now resistance markers that enzymatically inactivate the translation inhibitors hygromycin B and blasticidin S have been adapted for use in B. burgdorferi57. Since these drugs are not used clinically and exhibit minimal cross-resistance with the other markers, they can be exploited for the genetic manipulation of B. burgdorferi57. These laboratory tools will facilitate further research into B. burgdorferi infection and physiology, which will likely contribute to novel strategies to prevent and treat Lyme disease.

Conclusions

Although the word limit of this review requires that we not include the vast majority of recent publications, it is hoped that some major themes, advancements, and future directions are clear. Controversy surrounding genus nomenclature persists for now but hopefully will be resolved so energy can again be directed toward the biology of the organisms and the diseases they cause. Further research into the antigenic variation system encoded at the vls system is now enabled by PacBio long-read sequencing and the VAST program to track genetic changes in B. burgdorferi. The mechanisms behind this unique segmental gene conversion system, and therefore the antigenic variation system that allows B. burgdorferi to maintain infections, deserve more study. We look forward to seeing how this area of research unfolds and its implications for the biology of the Lyme disease agents. Similarly, we anticipate the identification and investigation of more genes essential to B. burgdorferi infectivity and virulence. In addition to increasing our understanding of B. burgdorferi biology, these studies may direct investigation of future vaccine targets. We also sought to highlight the protein and lipid characteristics of the B. burgdorferi membranes. In light of its existence as an extracellular pathogen, these characteristics and dynamic changes are significant for interactions of B. burgdorferi with its environment, be it tick or vertebrate. By extension, general understanding of the physiology of B. burgdorferi, obtained through the applications of newly pioneered laboratory techniques, will uncover the secrets of this fascinating spirochete.

Abbreviations

ANI, average nucleotide identity; SCID, severe combined immune deficiency; Tn-seq, transposon sequencing; VAST, Variable Antigen Sequence Tracer; WT, wild-type

Comments on this article Comments (0)

Version 1
VERSION 1 PUBLISHED 31 May 2019
Comment
Author details Author details
Competing interests
Grant information
Copyright
Download
 
Export To
metrics
Views Downloads
F1000Research - -
PubMed Central
Data from PMC are received and updated monthly.
- -
Citations
CITE
how to cite this article
Winslow C and Coburn J. Recent discoveries and advancements in research on the Lyme disease spirochete Borrelia burgdorferi [version 1; peer review: 5 approved]. F1000Research 2019, 8(F1000 Faculty Rev):763 (https://doi.org/10.12688/f1000research.18379.1)
NOTE: If applicable, it is important to ensure the information in square brackets after the title is included in all citations of this article.
track
receive updates on this article
Track an article to receive email alerts on any updates to this article.

Open Peer Review

Current Reviewer Status: ?
Key to Reviewer Statuses VIEW
ApprovedThe paper is scientifically sound in its current form and only minor, if any, improvements are suggested
Approved with reservations A number of small changes, sometimes more significant revisions are required to address specific details and improve the papers academic merit.
Not approvedFundamental flaws in the paper seriously undermine the findings and conclusions
Version 1
VERSION 1
PUBLISHED 31 May 2019
Views
0
Cite
Reviewer Report 31 May 2019
Catherine Brissette, Department of Biomedical Sciences, University of North Dakota School of Medicine and Health Sciences, Grand Forks, ND, USA 
Approved
VIEWS 0
I confirm that I have read this submission and believe that I have an ... Continue reading
CITE
CITE
HOW TO CITE THIS REPORT
Brissette C. Reviewer Report For: Recent discoveries and advancements in research on the Lyme disease spirochete Borrelia burgdorferi [version 1; peer review: 5 approved]. F1000Research 2019, 8(F1000 Faculty Rev):763 (https://doi.org/10.5256/f1000research.20108.r48770)
NOTE: it is important to ensure the information in square brackets after the title is included in all citations of this article.
Views
0
Cite
Reviewer Report 31 May 2019
D. Scott Samuels, Division of Biological Sciences, University of Montana, Missoula, MT, USA 
Approved
VIEWS 0
I confirm that I have read this submission and believe that I have an ... Continue reading
CITE
CITE
HOW TO CITE THIS REPORT
Samuels DS. Reviewer Report For: Recent discoveries and advancements in research on the Lyme disease spirochete Borrelia burgdorferi [version 1; peer review: 5 approved]. F1000Research 2019, 8(F1000 Faculty Rev):763 (https://doi.org/10.5256/f1000research.20108.r48768)
NOTE: it is important to ensure the information in square brackets after the title is included in all citations of this article.
Views
4
Cite
Reviewer Report 31 May 2019
Ira Schwartz, Department of Microbiology and Immunology, New York Medical College, Valhalla, NY, USA 
Approved
VIEWS 4
I confirm that I have read this submission and believe that I have an ... Continue reading
CITE
CITE
HOW TO CITE THIS REPORT
Schwartz I. Reviewer Report For: Recent discoveries and advancements in research on the Lyme disease spirochete Borrelia burgdorferi [version 1; peer review: 5 approved]. F1000Research 2019, 8(F1000 Faculty Rev):763 (https://doi.org/10.5256/f1000research.20108.r48766)
NOTE: it is important to ensure the information in square brackets after the title is included in all citations of this article.
Views
3
Cite
Reviewer Report 31 May 2019
Jon T Skare, Department of Microbial Pathogenesis and Immunology, College of Medicine, Texas A&M Health Science Center, Bryan, Texas, USA 
Approved
VIEWS 3
I confirm that I have read this submission and believe that I have an ... Continue reading
CITE
CITE
HOW TO CITE THIS REPORT
Skare JT. Reviewer Report For: Recent discoveries and advancements in research on the Lyme disease spirochete Borrelia burgdorferi [version 1; peer review: 5 approved]. F1000Research 2019, 8(F1000 Faculty Rev):763 (https://doi.org/10.5256/f1000research.20108.r48771)
NOTE: it is important to ensure the information in square brackets after the title is included in all citations of this article.
Views
0
Cite
Reviewer Report 31 May 2019
Steven J Norris, Department of Pathology and Laboratory Medicine, UTHealth McGovern Medical School, Houston, Texas, USA 
Approved
VIEWS 0
I confirm that I have read this submission and believe that I have an ... Continue reading
CITE
CITE
HOW TO CITE THIS REPORT
Norris SJ. Reviewer Report For: Recent discoveries and advancements in research on the Lyme disease spirochete Borrelia burgdorferi [version 1; peer review: 5 approved]. F1000Research 2019, 8(F1000 Faculty Rev):763 (https://doi.org/10.5256/f1000research.20108.r48769)
NOTE: it is important to ensure the information in square brackets after the title is included in all citations of this article.

Comments on this article Comments (0)

Version 1
VERSION 1 PUBLISHED 31 May 2019
Comment
Alongside their report, reviewers assign a status to the article:
Approved - the paper is scientifically sound in its current form and only minor, if any, improvements are suggested
Approved with reservations - A number of small changes, sometimes more significant revisions are required to address specific details and improve the papers academic merit.
Not approved - fundamental flaws in the paper seriously undermine the findings and conclusions
Sign In
If you've forgotten your password, please enter your email address below and we'll send you instructions on how to reset your password.

The email address should be the one you originally registered with F1000.

Email address not valid, please try again

You registered with F1000 via Google, so we cannot reset your password.

To sign in, please click here.

If you still need help with your Google account password, please click here.

You registered with F1000 via Facebook, so we cannot reset your password.

To sign in, please click here.

If you still need help with your Facebook account password, please click here.

Code not correct, please try again
Email us for further assistance.
Server error, please try again.