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Comparative evaluation of the effect of 2% graphene oxide and 5% hydroxyapatite nanoparticles in isolation and in combination on micro tensile bond strength of 5th generation adhesive

[version 1; peer review: 1 approved with reservations]
PUBLISHED 18 May 2023
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This article is included in the Datta Meghe Institute of Higher Education and Research collection.

Abstract

Graphene is the thinnest, strongest and stiffest imaginable material. The biocompatible property of graphene oxide can initiate and facilitate cell adhesion, proliferation and differentiation of periodontal ligament, osteogenic and oral epithelial cells. Furthermore, the antibiofilm and anti-adhesion properties of graphene oxide in prevention of dental biofilm infections, dental caries, dental erosion as well as for implant surface modification and as anti-quorum sensing agent.
Composites are most often utilised materials for restoration in the field of dentistry due to adhesive resins' improved mechanical and cosmetic properties. To safeguard the dentin and prevent dental cavities, dentin adhesives are utilised to affix hydrophobic resin composites to hydrophilic dentin tissue. Dental adhesives have a harder time adhering to dentin because it contains more water and is less mineralized than enamel. This makes the method more sensitive. As a result, it was chosen to assess and contrast the impact of 5% Hydroxyapatite nanoparticles and 2% Graphene oxide nanoparticles, both separately and together, on the Micro tensile bond strength of 5th generation adhesive. Graphene oxide is the most versatile form of Graphite in structural and functional configuration. Graphene oxide possess extraordinary physical, chemical, optical, electrical and mechanical properties. Among the graphene family nanomaterials, the reduced form of Graphite adding the oxygenated functional group to the structure increases the surface area and therefore exhibits enviable excellent interaction ability with metal and ions as well as organic species. Graphene oxide in dentistry has provided outstanding results in antimicrobial action, regenerative dentistry, bone tissue engineering, drug delivery, physicochemical property, enhancement of dental biomaterials and oral cancer treatment.

Keywords

Nanocrystals, Dental adhesive, Graphene oxide, Hydroxyapatite, Anti-microbial, Biocompatibility, Hydrophobic, Micro-tensile bond strength.

Introduction

Considering their enhanced aesthetics and increased mechanical qualities, the most used materials for restoration in dentistry is dental composite.1 Dentin adhesives are utilized to treat and prevent dental caries as well as to adhere hydrophobic resin composites to hydrophilic dentin tissue.2 Dental adhesives have a harder time adhering to dentin because it contains more water and is less mineralized than enamel. This necessitates meticulous handling as it is highly sensitive to the procedures being performed3 Effectiveness of adherence with dentin in tooth structure is related to the monomers' capacity to extend and enter the gaps between collagen fibers and the stability of the resin-based tags used to create a hybrid layer.4 Loss of adhesive bond over time is considered one of the prime causes of composite restorative failure leading to complications.5 Bond failure as well as the emergence leading to secondary caries are caused by loss of bond, which results in causing the production of nano-gaps.6 To boost dentin interactions with adhesive resins also maybe lessen dentin-adhesive degradation to counteract failure of adhesion over time, also use of inorganic fillers to adhesives is advised.7 Recent research has demonstrated that addition of fillers in adhesives can improve their mechanical qualities while reducing water sorption and solubility.8,9 Thus, it is extremely desirable to incorporate inorganic nanomaterials as fillers/particles to enhance the physicomechanical characteristics of bonding agents. In recent years, dental researchers have become interested in several materials, including graphene. Materials made of graphene have a large surface area therefore both are thermally and chemically stable.10 Graphene oxide (GO), a member of the graphene family of nanomaterials, is particularly interesting and is produced by oxidizing graphite.11 Graphene is typically thought of as a hydrophobic material, however, because GO has oxygen in its functional groups compared to other GFNs (graphene family nano particles) it is thought to be hydrophilic.12 Its hydrophilicity could be viewed as a positive trait because it aids in the formation of stable colloid dispersion so prevents aggregation, making GO more cytocompatible.13 Lee et al. incorporated GO into dentin adhesive successfully in 2018 despite using a mix of GO and bioactive glass since it has better antibacterial activity than graphite does.14 Also, the combination of GO with the remineralizing substance hydroxyapatite (HA) will be tested in this study to see how it affects the adhesive's characteristics. HA is an inorganic, non-toxic bioactive substance that is frequently found as a significant component of tooth structure and is available as nanoparticles.15 Due to HA's demonstrated ability to remineralize bone, the number of dental materials that include it has increased.16 Due to the dental adhesives' intimate contact with tooth dentin, incorporation of HA nanoparticles in resin adhesives aids in remineralizing the caries-affected dentin. Moreover, the hybrid dentin layer's interaction with HA to create an organic bond will make it more difficult for it to separate from the tooth.17 In a study performed earlier, Leitune et al. (2013) found that incorporating nanoparticles of HA in the dental adhesive increased the binding strength between the adhesive and the dentin.18 In a study performed earlier by Bin-Shuwaish et al. (2010) showed how GO particles might enhance the mechanical properties of glue.19 In a related earlier study, Mei et al. in 2017 found that the addition of 1 wt % GO-silica particles might enhance compressive strength of the adhesives.20 Also, our study compares the impact of 5% Hydroxyapatite nanoparticles and 2% Graphene oxide nanoparticles, onto micro tensile bond strength when used alone and together. It is anticipated that more GO particles will be added to enhance the experimental dentin's binding capacity, durability, and dentin interaction. The conventional dental adhesive systems clinically being used in dentistry and their physical and mechanical properties varied widely in terms of bond strength and adhesion at the dentin interface which further compromised the prognosis leading to failure of the treatment. To improvise the related required properties in the 5th generation adhesive system, the graphene oxide and hydroxyapatite nanoparticles will be incorporated considering their promising structural configuration additive physical and mechanical properties aiding in the enhancement of the dental material. The focus of our study is to evaluate increase in the Micro tensile Bond strength of the 5th Generation Adhesive system after the incorporation of the graphene oxide and hydroxyapatite nanoparticles. Adhesion of the nanoparticles at the dentin interface leading to widespread surface area may further contribute to the increased bond strength, enhancing the mechanical properties.

Discussion

Before materials are marketed in dentistry, it is crucial to conduct and analyze mechanical strength tests on them. Through laboratory investigations, these evaluations are carried out in a variety of ways. Using the TBS test method, the bonding strengths of self-adhesive composite resins with various universal bonding methods were examined in this study. The TBS values increased when the adhesive system was used with universal adhesive systems. As a result, our study's null hypothesis was rejected. The fifth-generation resin adhesive's bond integrity, longevity, and contact with dentin were studied in the current study in relation to the addition of nano-HA and different nano-GO particle concentrations (0.5 wt% and 2 wt%). The Hydroxyapatite-graphene oxide nanoparticles 2% group demonstrated greater micro tensile bond strength than the Hydroxyapatite-graphene oxide nanoparticles 0.5% group, which revealed greater than the controls, according to the data. The idea that adding graphene oxide nanoparticles to the bonding agent would strengthen its binding was therefore considered. Adhesives are essential for the clinical outcome of restorations because inadequate restorative adhesive bonds have been linked to secondary caries development, dentin hypersensitivity (DH), and microleakage.21 Dental composites' diverse mechanical and physical qualities are reinforced by the universal use of nanoparticles.22 The nano-HA particles have a great ability to connect with the dental tissues, are biocompatible, and serve as a source of remineralizing ions like Ca and P.23,24 According to the current study, adding nano-HA particles to the adhesive produces all the advantages of using HA in adhesives. Nano-HA particles have been proposed in the past as a way to improve mechanical characteristics of adhesive and enhance surface area for adhesion.25 This recommendation was carried out, and nano-HA particles were used in the current study (100 nm in size). Nano-GO particles were added to HA-containing glue at two different concentrations (0.5% and 2.0%) to further enhance the adhesive bonding properties of the experimental adhesives. The inclusion of foreign fillers in the glue must be evenly distributed to reap the full benefits. The optimal performance of the adhesive may be compromised by gaps or cracks created by an incomplete or partial diffusion of filler nanoparticles.26 Microorganisms can live in the dental plaque and produce pulpal irritation and dental cavities. According to a prior study by Cantore et al., in which an alcohol-free mouthwash shows comparable antibacterial effectiveness to regular antimicrobial mouthwash chlorhexidine and can also reduce the risk of plaque formation. Dental caries and pulpal inflammation are prevented by every component of an oral product that is antibacterial and can lower microbial burden.27 Key dental pathogens like Streptococcus mutans are not able to adhere to graphene-based dental adhesives.

Objectives

  • 1. To evaluate the micro tensile bond strength of the 5th generation bonding agent without incorporating any of the nanoparticles using a universal testing machine.

  • 2. To evaluate the micro tensile bond strength of a 5th generation bonding agent incorporating 2% Graphene oxide nanoparticles in isolation using a universal testing machine.

  • 3. To evaluate the micro tensile bond strength of 5th generation bonding agent incorporating 5% Hydroxy apatite nanoparticles in isolation using a universal testing machine.

  • 4. To compare the micro tensile bond strength of 5th generation bonding agent incorporating 2% graphene oxide and 5% Hydroxyapatite nanoparticles in combination using a universal testing machine.

TRIAL DESIGN: In-vitro study

Protocol

Materials required:

  • 1. Graphene oxide nanoparticles (Nano Research Lab, Jamshedpur, India)

  • 2. Hydroxyapatite nanoparticles (Nano Research Lab, Jamshedpur, India)

  • 3. 5th generation bonding agent (3M ESPE Adper Single Bond 2)

  • 4. Dentsplyspectrum - composite restoration

Selection Criteria

  • Inclusion criteria

  • 84 Maxillary premolars

  • Teeth devoid of restorations

  • Exclusion criteria

  • Teeth with previous root canal treatment.

  • Extensively carious tooth.

  • Abrasion, attrition, fluorosis, or other enamel defects.

  • Teeth with developmental anomalies.

  • Teeth with external and internal resorption.

Intervention

  • A total of 84 samples are used in the study divided into 4 groups, 21 in each group corresponding to the 1, 2, 3, and 4 sample sets.

  • Group 1 – 5th generation bonding agent

  • Group 2-5th generation bonding agent with graphene oxide nanoparticles.

  • Group 3-5th generation bonding agent with hydroxy apatite nanoparticles

  • Group 4-5th generation bonding agent with a combination of graphene oxide and hydroxy apatite nanoparticles.

Procedure:

1. Addition of HA to the dentin Adhesive

The nano-HA particles will be silanized after being purchased commercially. 5% silane will be added to the 95% adhesive to help the nano-hydroxyapatite stick to it.

Solvent for silanization using acetone. To create a fifth adhesive system that contains 5 wt% nano-Hydroxyapatite particles, the nano-HA particles will be introduced to the experimental adhesive at a 5% concentration (m/m). The experimental adhesive's nano-hydroxyapatite particles will be dispersed using sonication in a centrifuge to encourage homogenization. To allow the solvent to evaporate, the synthesized adhesives will be stored in isolation for 24 hours at 37 degrees oC. Due to their short shelf life, the adhesives will thereafter be stored at 4 degrees oC and used for the duration of 20 days after formulation.

  • 1. Adding Graphene oxide to the dentin Adhesive

    The graphene oxide powder will be combined with 2 mL of ethanol in a microbial and ultrasonically processed for 10 minutes at room temperature. The dentin adhesive will get this mixture. The nano-graphene oxide particles will first be combined with resin before being sonicated in an ultrasonic bath for 10 minutes so creating a uniform combination. After sonication, the mixture will be homogenized for 60 seconds at room temperature in an ultrasonic homogenizer using pulse on/off technology. Every time the mixture is used, the mix will be homogenized in an ultrasonic homogenizer to ensure that the graphene oxide will disperse uniformly after storage. Calculations will be done to determine the resin volume in milliliters and milligram weight of nanoparticles. Using the following:

Weight/volume%=weight of solute/volume of solution100
  • 2. Addition of GO and HA to the dentin Adhesive

    The graphene oxide powder will be combined with 2 mL of ethanol in a microbiological container before being ultrasonically processed for 10 minutes at room temperature. The nano-HA particles were silanized after being purchased commercially. The 95% acetone solvent will receive an addition of 5% silane for silanization to enhance the adherence of nano-Hydroxyapatite to the adhesive. Once both the nanoparticles are made, the made solvents will be added to the 5th generation dentin adhesive. To achieve a homogenous mixture, it will be sonicated in an ultrasonicator duration of 10 min. Post-sonication, mixture will be homogenized in ultrasonic homogenizer at pulse on/of for 60 s at room temperature. The mixture will be rehomogenized in the ultrasonic homogenizer before each and every use to ensure that graphene oxide and hydroxy apatite nanoparticles would disperse uniformly after storage. Calculations will be done to determine the resin volume in millilitres and milligrams weight of the nanoparticles. This will allow the solvent to evaporate, the synthesized adhesives will be stored in isolation for 24 hours at 37 degrees oC. Due to their short shelf life, the adhesives will thereafter be stored at 4 degrees oC and will be used within 20 days of the formulation.

The investigation will use extracted permanent maxillary premolars. All teeth will be kept in the storage media for a maximum of three months in distilled water. The 1, 2, 3, and 4 sample sets will be represented by the groups of the 84 premolars, which will be randomly divided into those groups. There will be Class 1 cavity preparation. The 3 M Scotchbond Universal Etchant (30 – 40% phosphoric acid) will be applied to all groups for 15 seconds. The surfaces will then be washed and dried by air. The surplus glue will then be removed using dental cotton rolls after adhesive systems have been placed for 15 seconds. The surfaces will then be exposed to light for nearly 20 seconds at an intensity of around 800 mW/cm2 using a light-curing device. The previously polymerized adhesive layer will be covered with successive 2-mm thick layers using Dentsply Spectrum -restorative material, which will then be light cured for 20 seconds. Following treatment, all of the teeth will be further separated into resin dentin sticks using a diamond discs and water cooling in accordance with ISO/TS 11405, with an average cross-sectional area of 1 mm by 1 mm. The samples will then be kept in deionized (DI) water for 24 hours at 37 °C. Next, utilizing universal testing machine, the created samples will go through a micro-tensile bond strength test. The beams will be secured to a jig with cyanoacrylate adhesive before being put to tensile stresses at a crosshead speed of one millimeter per minute.

Sample size

Primary Variable - Microtensile bond strength

Sample size formula for difference between two means:

N=+2δ2Δ2

Where;

Zα = level of significance at 5% i.e., 95%

Confidence interval = 1.96

Zβ is the power of test =80% =0.84

δ1 =SD of MTBS in group 1 =29.74 ± 3.81

δ2 = SD of MTBS in group 2 =25.21 ± 3.60

= Difference between two means

=4.53

Pooled std. dev.= (3.81+3.60)/2 =3.71

n=1.96+0.8423.7124.532=15

n =15 teeth needed in each group

Total sample size is 60

Study Reference: [28]

STATISTICAL METHOD: All the results will be calculated using SPSS version 27 software, a proprietary alternative in which the analysis can be carried out is R Studio. Data for outcomes variables will be tested for normality using kalmogorov-smirnov. The comparative analysis of the micro tensile bond strength will be evaluated on the measurement of Newton. ANOVA will be used to find the significant difference between the mean of the 4 groups. Tukey test will be used for comparative evaluation of measurement in between 2 groups pairwise. P- value ≤ 0.05 will be considered as significant at 5% level of significance and 95% confidence of interval.

Conclusions

It is expected that among the material used in the study, the 5th generation bonding agent with graphene oxide and nano hydroxyapatite crystals in combination may give better results as compared to the 5th generation bonding agent with or without incorporation of graphene oxide and nano hydroxyapatite crystals in isolation in routine practice due to its ability of anti-microbial properties, optimal biocompatibility, and increased bond strength.

Dissemination

The focus of our study is to evaluate increase in the micro tensile bond strength of the 5th generation adhesive system after the incorporation of the graphene oxide and hydroxyapatite nanoparticles. Adhesion of the nanoparticles at the dentin interface leading to widespread surface area may further contribute to the increased bond strength, enhancing the mechanical properties.

Study status

Not started yet.

Ethical considerations

Ethical approval received from Datta Meghe Institute of Higher Education and Research, Sawangi, Wardha

IEC reference number- DMIHER (DU)/IEC/2023/584

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Kriplani S and Sedani S. Comparative evaluation of the effect of 2% graphene oxide and 5% hydroxyapatite nanoparticles in isolation and in combination on micro tensile bond strength of 5th generation adhesive [version 1; peer review: 1 approved with reservations]. F1000Research 2023, 12:514 (https://doi.org/10.12688/f1000research.133454.1)
NOTE: If applicable, it is important to ensure the information in square brackets after the title is included in all citations of this article.
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Open Peer Review

Current Reviewer Status: ?
Key to Reviewer Statuses VIEW
ApprovedThe paper is scientifically sound in its current form and only minor, if any, improvements are suggested
Approved with reservations A number of small changes, sometimes more significant revisions are required to address specific details and improve the papers academic merit.
Not approvedFundamental flaws in the paper seriously undermine the findings and conclusions
Version 1
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PUBLISHED 18 May 2023
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Reviewer Report 14 Nov 2023
Ranj Nadhim Salaie, Tishk International University, Erbil, Kurdistan Region, Iraq 
Approved with Reservations
VIEWS 26
1- Proof reading is required, inappropriate use of grammar, punctuation in some sentences

2- Where are the micro tensile bond strength data? there must be some graphs from the universal tensile machine software. 

3- ... Continue reading
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CITE
HOW TO CITE THIS REPORT
Salaie RN. Reviewer Report For: Comparative evaluation of the effect of 2% graphene oxide and 5% hydroxyapatite nanoparticles in isolation and in combination on micro tensile bond strength of 5th generation adhesive [version 1; peer review: 1 approved with reservations]. F1000Research 2023, 12:514 (https://doi.org/10.5256/f1000research.146445.r204068)
NOTE: it is important to ensure the information in square brackets after the title is included in all citations of this article.
  • Author Response 27 Nov 2023
    Simran Kriplani, Conservative dentistry and Endodontics, Sharad Pawar Dental College, Datta Meghe Institute of Higher Education and Research, Wardha, 442001, India
    27 Nov 2023
    Author Response
    1. Proofreading is required, inappropriate use of grammar, and punctuation in some sentences
    Answer for the first query - The grammar correction and the proofreading have been done. ... Continue reading
  • Reviewer Response 14 Jun 2024
    Ranj Nadhim Salaie, Tishk International University, Erbil, Iraq
    14 Jun 2024
    Reviewer Response
    Accepted
    Competing Interests: No competing interests were disclosed.
COMMENTS ON THIS REPORT
  • Author Response 27 Nov 2023
    Simran Kriplani, Conservative dentistry and Endodontics, Sharad Pawar Dental College, Datta Meghe Institute of Higher Education and Research, Wardha, 442001, India
    27 Nov 2023
    Author Response
    1. Proofreading is required, inappropriate use of grammar, and punctuation in some sentences
    Answer for the first query - The grammar correction and the proofreading have been done. ... Continue reading
  • Reviewer Response 14 Jun 2024
    Ranj Nadhim Salaie, Tishk International University, Erbil, Iraq
    14 Jun 2024
    Reviewer Response
    Accepted
    Competing Interests: No competing interests were disclosed.

Comments on this article Comments (0)

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Comment
Alongside their report, reviewers assign a status to the article:
Approved - the paper is scientifically sound in its current form and only minor, if any, improvements are suggested
Approved with reservations - A number of small changes, sometimes more significant revisions are required to address specific details and improve the papers academic merit.
Not approved - fundamental flaws in the paper seriously undermine the findings and conclusions
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