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Research Article
Revised

Malware Detection Using RNA Encoding and Convolutional Neural Networks on the Malicious Network Dataset

[version 2; peer review: 2 approved with reservations]
PUBLISHED 05 May 2026
Author details Author details
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This article is included in the Software and Hardware Engineering gateway.

This article is included in the Fallujah Multidisciplinary Science and Innovation gateway.

Abstract

Background

The detection of malware in network traffic remains a critical cybersecurity challenge. Traditional signature-based intrusion detection demonstrates a high level of familiarity with issues that have been recorded in the database; but show significantly lower effectiveness when it comes to polymorphic or zero-day attacks. Conversely, anomaly-based approaches are also endowed with the ability to detect new incursions, but often have a high false-positive rate.

Methods

This study proposes a combined malware-detection framework which makes use of RNA encoding network-flow attributes alongside Convolutional Neural Network (CNN) classifiers. The framework has three functionalities: a Signature-CNN, which is trained on RNA-encoded representation of known malicious flows; an Anomaly-CNN, which is developed to distinguish between benign and malicious traffic without any signature prior knowledge; and a Hybrid-CNN, which combines both paradigms in a two-stage detection pipeline.

Results

The research is carried out on the 10,000 samples that are split into training and testing subsets based on the 70/30 split strategy. The given model is trained in the context of a supervised learning model and assessed in terms of common performance metrics, such as accuracy, precision, recall, and F1-score. The experimental design is written in Python and deep learning libraries, so that the evaluation environment of all experiments is consistent and reproducible. Experiments conducted on the Malicious Network Dataset show that the Signature-CNN achieves 91% accuracy with strong precision on known threats, the Anomaly-CNN achieves 93% detection rate on unknown malware, and the Hybrid-CNN achieves the best overall performance with 95% detection rate and 94.5% F1 score.

Conclusions

The results demonstrate that RNA encoding combined with CNN classifiers offers a robust and scalable solution for malware detection in networked environments.

Keywords

Malware Detection, RNA Encoding, Convolutional Neural Networks, Network Security, Malicious Network Dataset

Revised Amendments from Version 1

The new copy of this manuscript includes some key changes based on the reviewer feedbacks. The abstract has been extended with more information about the experimental design and evaluation plan, which makes it more comprehensive and clear. Introduction has been well edited to enhance the quality of language, readability and academic tone.
The limitation of the existing studies has been emphasized and a more critical analysis of the existing studies has been added to the Related Work section to clearly establish the limitation of the existing studies and to position the contribution of the proposed method in a better manner. In addition, the Methodology section is now expanded with specific training parameters of CNN model including the optimizer, learning rate, number of epochs, and batch size to facilitate the reproducibility.
The Discussion has been greatly expanded with more information about how RNA-based encoding can be used to better represent features and the overall model performance. Lastly, the Conclusion has been revised to incorporate additional specific and practical future research directions, including possible extensions and implementations of the proposed approach.
All these updates enhance the clarity, rigor and scientific contribution of the manuscript.

See the authors' detailed response to the review by Atif Raza Zaidi
See the authors' detailed response to the review by Mohammed Subhi

1. Introduction

The exponential growth of networked systems proliferation has led to an equivalent increase in advanced malware attacks. Intrusion detection systems (IDS) continue to play a central role in the protection of digital infrastructure; however, modern practices are characterized by severe limitations.1 Signature-based IDS rely on a set of pre-defined rules or patterns, and these systems are effective only against previously identified threats. Anomaly-based IDS attempt to detect deviations from normal behavior, and has the ability to detect zero-day attacks, but often generate a high false positive. To address these limitations, a hybrid architecture has arisen, combining the benefits of each of the system.2,3

There have been recent studies into how deep-learning architectures, especially convolutional neural networks (CNNs) and recurrent neural networks (RNNs), can be applied to the problems of IDS, with promising results. A novel intrusion detection method based on learning framework is proposed,4 where the proposed method is done by using dual parallel CNN pipelines to independently address the network and radar features. A new intrusion detection model is suggested by,5 where this model combines CNN and Random Forest. The CNN is utilized to extract the feature, and the Random Forest is used for classification. An IDS is proposed by combining an innovative hybrid Autoencoder with an enhanced LSTM-CNN architecture,6 where the proposed method can enhance the detection capabilities more quickly and efficiently. Kaissar et al.7 investigates the optimization of hyperparameters in CNN to enhance the NIDS performance, where Grid Search, Genetic Algorithm, Particle Swarm Optimization, and Grey Wolf Optimization algorithms are used for this purpose. Alrayes et al.8 suggested a novel IDS by combining channel attention and CNN, where the suggested method has exceptional accuracy when applied it to NSL-KDD dataset. A new IDS model is built based on CNN and knowledge distillation,9 this model using two-dimensional Fourier transform for converting the grayscale images to the frequency domain, and this led to enhanced the similarity between neighboring pixels to address data effectively. Ban et al.10 suggested an enhanced deep-learning model for IDS in IoT environment, where the suggested model is depending on CNN as the backbone network in the constructed model. A hybrid deep learning IDS is proposed by11 based on CNN and bidirectional long short-term memory neural networks, where the proposed system is enhanced the model’s ability to detect patterns in both minority and majority classes. Altunay and Albayrak12 developed IDS in the IIoT networks, where the suggest system is done by using three different deep learning architectures, which are CNN, Long Short-Term Memory (LSTM), and the combination of these two methods.

Parallel Encodings Biologically inspired encodings, like mappings to DNA and RNA sequences, have been proposed to convert heterogeneous data to symbolic strings.13,14 Nevertheless, there is limited evidence in the extant literature of the integration of RNA encodings with CNN classifiers in the entire range of detection paradigms: signature, anomaly, and hybrid. The current paper seals this gap by suggesting a CNN-based model which incorporates these complementary detection schemes. Despite the good outcomes of the current methods, a number of shortcomings still exist. Most CNN-based approaches use traditional data representations, which might not best represent intricate feature interactions, leading to worse performance in adverse conditions. Moreover, certain methods have higher computational costs and reduced resilience to a variety of data or when used on noisy data. These drawbacks indicate why encoding methods should be more effective and articulate. Here the proposed approach refers to encoding based on RNA to increase the feature representation to allow the model to learn more discriminative features to enhance the performance of the model in comparison with the current methods.

2. Methodology

The proposed malware detection system is built by combining of RNA-inspired encoding of the network traffic characteristics and the convolutional neural network (CNN) classification. Unlike traditional intrusion detection system models that treat signature-based and anomaly-based detection methodologies as dissimilar entities, the current system integrates both of them in a single deep learning pipeline. In this pipeline, CNN models that are trained on sequences coded using the RNA-inspired methodology concurrently address signature-based, anomaly-based, and hybrid detection. The steps of the proposed system are shown in Figure 1.

eab33da7-608f-4884-b7fe-0961fb4aa37e_figure1.gif

Figure 1. Flow chart of the suggested malware detection system with RNA encoding and CNN classification pipeline.

2.1 Dataset description

The Malicious Network Dataset is a new dataset that was collected using honeypots deployed with the Honeytrap agent. The dataset consists of 9 features that represent various aspects of network traffic, including both structural and payload data.15 These features are shown in Table 1 as follows:

Table 1. The malicious network dataset values and its descriptions.15

Feature ColumnPossible values
1 Protocol TCP, IP
2 remote_ip Too many unique values (10,951). Example: ['35.203.211.180', '180.93.172.180', '81.17.19.66', '0.0.0.0', '94.23.145.155']
3 remote_port Numeric range: 0 – 65535 (unique=28,920)
4 local_ip Too many unique values (4,030). Example: ['165.227.180.71', '0.0.0.0', '192.168.202.139', '157.240.11.61', '192.168.11.143']
5 local_port Numeric range: 0 – 65535 (unique=11,985)
6 md5_hash Too many unique values (13,732). Example: ['1fb4aeaab94ca27d2e5dfaa47e11a6fb', 'd41d8cd98f00b204e9800998ecf8427e', '19b893b938ace1defe7d090e510f0618', “, '59b490c4ab003464ca03428b3fc63222']
7 sha512_hash Too many unique values (13,732). Example: ['a6d4f36a2a8d5b5ea7e6afe91d4a80e7a9ae2129ebf4791a4d74b7ea003420c2…', 'cf83e1357eefb8bdf1542850d66d8007…', 'f060846cbf02e31706d5d0fe781d7007…', “, '922450f93e933de877934cee97339ae6…']
8 Length Numeric range: 0 – 1448 (unique = 1,038)
9 data_hex Too many unique values (13,732). Example: ['16030100ca010000c60303918984ed51d5c0b8d4cfad730a16a4efb24b004062e21…', “, '50100', '0', '474554202f20485454502f312e310d0a486f7374…']
10 Class 'malicious' or 'benign'.

2.2 RNA encoding of network features

Network flows in the Malicious Network Dataset comprise heterogeneous attributes, such as protocol types, port numbers, cryptographic hashes, packet lengths, and payloads. Such attributes are of different scales and representation thus complicating direct modeling. In this spirit we introduce a biologically inspired RNA encoding scheme where each element is coded to a fixed set of codons. Where the mapping rules as follow:

  • Remote ip and local ip attributes were eliminated, because a malware detection model must identify malicious signatures regardless of the source and destination IP addresses, and these ips do not provide meaningful behavioral indicators of malware.

  • Protocol identifiers (e.g., TCP, IP) are assigned codons such as TCP → G, IP → U.

  • Numerical fields (ports, lengths) are separated into digits, each mapped to a codon, where each digit is represented by two RNA characters, e.g., 0 → CG, 1 → AC, and so on.

  • Hexadecimal payload values are mapped similarly, where each character is represented by two RNA characters, e.g., a → AU, b → UU, and so on.

  • The hash values, containing both MD5 and SHA512 are divided into single characters and coded into codons, thus, making sure that each unique character is represented by a deterministic codon.

  • For each flow, codon sequences from all fields are concatenated in the following order: [protocol] → [remote port] → [local port] → [MD5] → [SHA512] → [length] → [payload].

  • The built RNA encoding for all possible malicious network dataset records values is shown in Table 2.

Table 2. RNA encoding for malicious network dataset records values.

Value RNA encoding
TCPG
IPU
0CG
1AC
2GG
3UA
4CC
5GA
6UC
7AA
8GU
9UG
aAU
bUU
cCA
dAG
eCU
fGC

This mapping transforms a wide range of categorical and string features into structured RNA codon sequences, thus making training of convolutional neural networks on homogeneous sequential inputs possible.

2.3 CNN architecture

The coded messages are fed into a Convolutional Neural Network (CNN) that picks up discriminative features at a variety of levels of abstraction as follow:

  • Embedding: The codons are first mapped to dense vectors of dimension, d = 32. This embedding is learnt alongside the classifier, thus, encoding similarities between codons.

  • Convolutional: A number of one-dimensional convolutional layers are used, the size of which varies between 5 and 7. These filters identify a local pattern in the codon sequences e.g., repeated sequences which can be an indication of malicious activity. An example would be to have a convolution filter that is trained to identify the codon sequence of known back door ports.

  • Pooling: The feature maps are down sampled through max-pooling, and the most conspicuous features are retained, with a lower computational cost.

  • Global Average Pooling: In order to generalize over a wide range of lengths of variable sequences and reduce overfitting, global average pooling is done to aggregate the feature maps into fixed-size vectors.

  • Dense Layers: The learned features are combined in fully connected layers (64 units) that use ReLU activation. To inhibit overfitting, dropout regularization is used, with a temporary activation of neurons in the course of training, i.e. p = 0.5.

  • Output Layer: One sigmoid neuron generates a probability score which can mark a sample to be benign or malicious.

  • The CNN architecture is applied for three different methods, and these methods are shown in Figure 2.

eab33da7-608f-4884-b7fe-0961fb4aa37e_figure2.gif

Figure 2. The three CNN models (Signature, Anomaly, and Hybrid).

In Signature-CNN, the Signature- CNN replaces traditional rule-based signature matching with a convolutional neural network, which is trained on representations of known patterns of malicious activity encoded by RNA. Instead of searching manually through the collection of byte sequences or hash values, the network is trained to identify codon-level motifs which are indicative of malicious flows. While the Anomaly- CNN is trained to identify deviation with the normal network behavior using sequences encoded by RNA. It is not based on predefined attack patterns as compared to the signature model. Finally, the Hybrid-CNN combines the two methods in two-staged pipeline, allowing the Signature-CNN to combine the precision of Anomaly-CNN with the generalization capability. The comparison between the three CNN models is clarified in Table 3.

Table 3. Comparison between different CNN models.

ModelInputObjectiveStrengthsWeaknesses
Signature-CNN RNA-coded sequences of known malicious and benign flowsDetect known attack patterns using learned codon-level motifsHigh precision and low FARCannot detect zero-day threats
Anomaly-CNN RNA-coded mixture of benign and malicious flowsIdentify deviations from normal codon distributionsDetects novel and polymorphic malwareHigher false-positive rate
Hybrid-CNN Two-stage input: Signature-filtered and residual flowsCombine the benefits of both precision and generalizationBest overall accuracy and balanceSlightly higher computational cost

Where the sequence handling all the RNA sequences were truncated or zero padded to a constant length of 2,048 codons to make the input equal. Also, the architectural consistency for the CNN models of the three models have the same architecture (three Conv1D layers with 64-128-256 filters, kernel = 5, ReLU activation, and dropout = 0.5). It is only in training objectives that there is a difference. Finally, the dataset was stratified 70/30 and sampled to maintain the malicious/benign ratio (50/50). These explanations guarantee the maximum reproducibility of the experiment. The CNN model is optimized through Adam optimization algorithm of learning rate 0.001. The training is undertaken through 50 epochs having a batch size that is 32. In a bid to reduce overfitting, dropout regularization, with a dropout rate of 0.5, is used, and early stopping is utilized, on the basis of validation loss. The model is optimized with categorical cross-entropy loss function and the performance of the model is continuously checked on a validation dataset during the training process to ascertain stability and convergence.

3. Results and discussion

The performance of the proposed CNN-based malware detection models is evaluated based on several standard classification metrics were employed, where these metrics are defined and calculated as follow:

  • Accuracy: Is the ratio of correctly labeled flows to the total number of flows, and it calculate based on the following equation:

    Accuracy=TP+TNTP+TN+FP+FN

  • Detection Rate (Recall): This rate also known as the True Positive Rate (TPR) is a metric that measures the rate of true malicious flows that are detected, and it calculate based on the following equation:

    Detection Rate(Recall)=TPTP+FN

  • Precision: Refers to the ratio of the flows that are predicted to be malicious which actually are malicious, and it calculate based on the following equation:

    Precision=TPTP+FP

  • F1 Score: Is the harmonic mean of Precision and Recall that provides a balanced assessment when there is a tradeoff between the two measures, and it calculate based on the following equation:

    F1Score=2×Precision×RecallPrecision+Recall

  • False Positive Rate (FPR): This value is used to estimate the ratio of false positive results of disproving benign flows, and it calculate based on the following equation:

    FPR=FPFP+TN

The identified enhancement of the performance with the help of RNA encoding can be explained by the capacity of the solution to increase the feature representation in the CNN framework. RNA encoding converts the input data into structured and biologically inspired encoding, which adds further diversity and non-linearity to the feature space. This transformation allows the network to pick up more complex and discriminative patterns that might not be available with traditional encoding methods. Additionally, RNA encoding also helps in reducing noise and enhancing generalization as it focuses on meaningful relationships in the data. Due to this, the CNN can develop more resilient features resulting in higher classification accuracy and system performance. The Malicious Network Dataset is used for evaluation the proposed method, where each method (signature, anomaly, or hybrid) is starting by preprocessing the used dataset by removing IP fields, then RNA encoding is applied to all remaining features. Then divided the dataset into training and testing, the training is equal to 70%, while the testing used the rest 30% from the whole dataset. The achieved results for the first method (signature-CNN) are shown in Table 4. The Signature-CNN was very accurate and reported a low false-positive rate, thus, justifying its accuracy in identifying known malware.

Table 4. The performance of the Signature-CNN model.

Metric Result
Accuracy0.915
Detection Rate0.89
Precision0.92
F1 Score0.90
FPR0.05

While the obtained results based on the second method (anomaly-CNN) are shown in Table 5. The Anomaly-CNN was able to achieve higher rate of detection, which revealed that zero-day threats can be spotted with a slight increase in false positives .

Table 5. The performance of the Anomaly-CNN model.

Metric Result
Accuracy0.93
Detection Rate0.93
Precision0.91
F1 Score0.92
FPR0.07

On other hand, when utilized the third method (hybrid-CNN), the achieved results are shown in Table 6. Hybrid-CNN delivered the best trade-off, achieving the best detection rate and F1 score whilst reducing false positives at the same time.

Table 6. The performance of the Hybrid-CNN model.

Metric Result
Accuracy0.95
Detection Rate0.95
Precision0.94
F1 Score0.945
FPR0.03

Finally, the comparison between the performance of all models (signature, anomaly, and hybrid) are shown in Table 7 and Figure 3.

Table 7. Performance of CNN models.

MethodAccuracyDetection ratePrecisionF1 score FPR
Signature-CNN 0.910.890.920.9050.05
Anomaly-CNN 0.930.930.910.920.07
Hybrid-CNN 0.950.950.940.9450.03
eab33da7-608f-4884-b7fe-0961fb4aa37e_figure3.gif

Figure 3. Comparison of Signature-CNN, Anomaly-CNN and Hybrid-CNN on the malicious network dataset.

As shown in Table 7, the achieved results based on hybrid-CNN are achieved the best results over the two others methods, where the obtained accuracy, detection rate, precision, F1 score, and FPR are equal to 95%, 95%, 94%, 94.5%, and 3% respectively. The Hybrid-CNN may be explained by the possibility of making decisions in two stages. The first stage filters familiar malicious patterns and the latter extrapolates to unknown codon patterns. This is a combination that reduces the antagonism of sensitivity and specificity. As compared to the Anomaly- CNN, however, it has a slightly higher recall with more false positives since it does not identify exact patterns but only the deviations. The Signature-CNN is also accurate to known dangers but does not have generalization and this is the reason behind its slightly lower recall.

The proposed method achieved results are compared with classical machine learning models (Random Forest, and XGBoost) and deep models (RNN, CNN-BiLSTM and AE-LSTM) and this comparison is shown in Table 8.

Table 8. Comparison of proposed models with existing baselines.

ModelAccuracyDetection ratePrecisionF1 score FPR
Random Forest0.870.840.860.850.09
XGBoost0.890.870.880.8750.08
RNN0.910.890.900.8950.07
LSTM0.920.900.910.9050.06
CNN-BiLSTM (Wang et al., 2024)0.930.920.920.920.05
Hybrid AE-LSTM (Xue et al., 2025)0.940.930.930.930.04
Hybrid-CNN (Proposed) 0.95 0.95 0.94 0.945 0.03

As shown in Table 8, The Hybrid-CNN achieved better results than the traditional and deep learning methods. Where the proposed method achieved the highest accuracy, detection rate, precision, and F1-score, where these results are equal to 95%, 95%, 94%, and 94.5% respectively. Also, the obtained FPR results are the lowest and equal to 3%. This has been enhanced by the fact that it has RNA encoding that maintains semantical links between traffic features and increases the pattern recognition capability of the CNN.

3.1 Computational efficiency

The experiments were being carried out on the NVIDIA RTX-4090 graphics card with 24 GB VRAM. The Signatures CNN took 1.8 hours for training, Anomaly CNN 2.3 hours and the Hybrid CNN 2.7 hours to training. The mean inference latency (per flow) was 2.1 ms, 2.4 ms and 3.0 ms respectively. Despite the fact that Hybrid-CNN is the most expensive in terms of computation since it involves two stages of evaluation, it is still capable of deployment in near-real-time and takes much less time than recurrent models, including LSTM and AE-LSTM.

4. Conclusion

This work proposed an integrated malware detector model based on CNN, which was built on RNA encoding and implemented on Malicious Network Dataset. Restructuring signature, anomaly, and hybrid detection as CNN-based paradigms, the system achieves strong performance across all detection modes. The Hybrid-CNN achieved the best results, having 95% of detection, and the same time, minimized false-positive risks. Future directions will be to extrapolate the proposed technique to bigger and more heterogeneous datasets to further test the generalization capability of the technique. Moreover, hybrid deep learning models, including CNN architecture and the use of transformer-based methods, will be considered to improve feature learning. The other valuable direction is the optimization of the model to real-time applications and minimization of the complexity of the computation. Also, the exploration of other bio-inspired encoding methods can offer further enhancements in feature representation and efficiency of the model.

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Fitian Rashid O, Ali Abd S and Al-Shahwani H. Malware Detection Using RNA Encoding and Convolutional Neural Networks on the Malicious Network Dataset [version 2; peer review: 2 approved with reservations]. F1000Research 2026, 15:241 (https://doi.org/10.12688/f1000research.173837.2)
NOTE: If applicable, it is important to ensure the information in square brackets after the title is included in all citations of this article.
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ApprovedThe paper is scientifically sound in its current form and only minor, if any, improvements are suggested
Approved with reservations A number of small changes, sometimes more significant revisions are required to address specific details and improve the papers academic merit.
Not approvedFundamental flaws in the paper seriously undermine the findings and conclusions
Version 2
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Reviewer Report 26 May 2026
Atif Raza Zaidi, TIMES University, Multan, Pakistan 
Approved with Reservations
VIEWS 23
The manuscript presents an interesting and relevant study on malware detection using RNA-inspired encoding with CNN-based models. The topic is relevant to network security and malware detection. The proposed approach is interesting, and the reported results are encouraging. However, the ... Continue reading
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Zaidi AR. Reviewer Report For: Malware Detection Using RNA Encoding and Convolutional Neural Networks on the Malicious Network Dataset [version 2; peer review: 2 approved with reservations]. F1000Research 2026, 15:241 (https://doi.org/10.5256/f1000research.199234.r483152)
NOTE: it is important to ensure the information in square brackets after the title is included in all citations of this article.
  • Author Response 03 Jun 2026
    Omar Fitian Rashid, Department of Geology, University of Baghdad, Baghdad, Iraq
    03 Jun 2026
    Author Response
    Reviewer Comments

    Comment 1: The RNA-inspired encoding is a central contribution of the paper. Therefore, an ablation experiment comparing CNN with RNA encoding and CNN without RNA encoding would ... Continue reading
COMMENTS ON THIS REPORT
  • Author Response 03 Jun 2026
    Omar Fitian Rashid, Department of Geology, University of Baghdad, Baghdad, Iraq
    03 Jun 2026
    Author Response
    Reviewer Comments

    Comment 1: The RNA-inspired encoding is a central contribution of the paper. Therefore, an ablation experiment comparing CNN with RNA encoding and CNN without RNA encoding would ... Continue reading
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Reviewer Report 21 Apr 2026
Mohammed Subhi, Directorate of Private University Education,, Baghdad, 10011,, Iraq;  Department of Cybersecurity/ Directorate of Information Technology, Iraqi Ministry of Higher Education and Scientific Research, Baghdad, Baghdad, Iraq 
Approved with Reservations
VIEWS 18
The manuscript presents an interesting and timely study on malware detection using RNA encoding combined with CNN architectures. The idea of integrating biologically inspired encoding with deep learning models is promising and shows potential for improving detection performance. However, several ... Continue reading
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Subhi M. Reviewer Report For: Malware Detection Using RNA Encoding and Convolutional Neural Networks on the Malicious Network Dataset [version 2; peer review: 2 approved with reservations]. F1000Research 2026, 15:241 (https://doi.org/10.5256/f1000research.191689.r474768)
NOTE: it is important to ensure the information in square brackets after the title is included in all citations of this article.
  • Author Response 05 May 2026
    Omar Fitian Rashid, Department of Geology, University of Baghdad, Baghdad, Iraq
    05 May 2026
    Author Response
    Reviewer Comments
    Comment 1:
    The abstract is generally well-structured; however, it would benefit from including additional details such as the dataset size and key aspects of the experimental setup to ... Continue reading
COMMENTS ON THIS REPORT
  • Author Response 05 May 2026
    Omar Fitian Rashid, Department of Geology, University of Baghdad, Baghdad, Iraq
    05 May 2026
    Author Response
    Reviewer Comments
    Comment 1:
    The abstract is generally well-structured; however, it would benefit from including additional details such as the dataset size and key aspects of the experimental setup to ... Continue reading

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Alongside their report, reviewers assign a status to the article:
Approved - the paper is scientifically sound in its current form and only minor, if any, improvements are suggested
Approved with reservations - A number of small changes, sometimes more significant revisions are required to address specific details and improve the papers academic merit.
Not approved - fundamental flaws in the paper seriously undermine the findings and conclusions
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