Keywords
Capsaicin, vascular smooth muscle cells, restenosis, proliferation
Capsaicin, vascular smooth muscle cells, restenosis, proliferation
Aberrant and accelerated VSMC proliferation is a main contributor to restenosis, the pathological re-narrowing of the vessel lumen after surgical interventions combating vascular stenosis. To overcome restenosis, drug-eluting stents have been developed, aiming at inhibiting VSMC growth by the release of anti-proliferative substances such as paclitaxel and rapamycin. However, these compounds display unresolved issues such as impaired re-endothelialization and subsequent thrombosis induction1, which makes the characterization of other compounds able to suppress VSMC proliferation highly relevant. Plant-derived natural products are an excellent resource for identifying lead compounds2. Here we examine the anti-proliferative potential of capsaicin, a bioactive component of chili peppers [Capsicum spp. (Solanaceae)], in VSMC.
To test whether capsaicin is able to inhibit proliferation of VSMC induced by PDGF, a major growth factor implied in the aberrant proliferative responses in restenosis3, the total amount of metabolically active cells was measured after 48 h of incubation by the resazurin conversion method4. Capsaicin indeed suppressed VSMC proliferation concentration-dependently with an IC50 of 5.36 μM (Figure 1A). To confirm the anti-proliferative effect of capsaicin with a second experimental method, we measured DNA synthesis in VSMC by quantification of 5-bromo-2′-deoxyuridine (BrdU) incorporation into DNA. Capsaicin also inhibited PDGF-stimulated DNA synthesis in a concentration-dependent manner with an IC50 of 3.81 μM (Figure 1B). To assure that the decreased number of VSMC upon treatment with capsaicin is not due to cytotoxicity, we quantified cell death by measuring cell membrane integrity estimated by lactate dehydrogenase (LDH) activity inside cells and in cell supernatants. No significant cytotoxicity was detected in the investigated concentration range (Figure 1C). In summary, capsaicin is identified as an inhibitor of VSMC proliferation. Further studies are prompted to elaborate the underlying mode of action of this natural product and to investigate its effect in advanced in vivo anti-restenotic models.
Cell proliferation was estimated by quantification of metabolic activity (A) and DNA synthesis (B). Cell death was estimated by quantification of the percentage of extracellular LDH (C). Data represent mean ± SD from at least three independent experiments (n.s., not significant; ***p < 0.001; **p < 0.01; ANOVA/Bonferroni).
Rat aortic VSMC used in this study were purchased from Lonza (Braine-L’Alleud, Belgium) and cultivated in DMEM–F12 (1:1) medium supplemented with 20% fetal calf serum and gentamycin. Capsaicin and other chemicals were obtained from Sigma-Aldrich (Vienna, Austria).
For the resazurin conversion assay, VSMC were seeded in 96-well plates at 5 × 103 cells/well. 24 h later, cells were serum-starved for 24 h to render them quiescent. Quiescent cells were pretreated for 30 min with capsaicin or vehicle (0.1% DMSO) as indicated, and subsequently stimulated for 48 h with PDGF-BB (20 ng/mL). To measure the number of metabolically active VSMC by resazurin conversion4, cells were washed with PBS and incubated in serum-free medium containing 10 μg/mL resazurin for 2 h. Total metabolic activity was measured by monitoring the increase in fluorescence at a wavelength of 590 nm using an excitation wavelength of 535 nm in a 96-well plate reader (Tecan GENios Pro).
For the BrdU incorporation assay, VSMC were seeded and starved as for the resazurin conversion assay. Quiescent cells were pretreated for 30 min with capsaicin, or vehicle as indicated and subsequently stimulated with PDGF-BB (20 ng/mL). To estimate de novo DNA synthesis in VSMC5, BrdU was added 2 h after PDGF stimulation, and the incorporated amount was determined 22 h afterwards with a BrdU ELISA kit according to the manufacturer’s instructions (Roche Diagnostics).
For assessing cytotoxicity, VSMC were seeded and serum-starved as indicated above. The quiescent cells were pretreated for 30 min with capsaicin, or vehicle as indicated, and subsequently stimulated for 24 h with PDGF-BB (20 ng/mL). To quantify the loss of cell membrane integrity as a sign for cell death6, the supernatants of the treated cells were assessed for LDH activity. For estimation of the total LDH, identically treated samples were incubated for 45 min in the presence of 1% Triton X-100. The released and total LDH enzyme activity was quantified for 30 min in the dark in the presence of 4.5 mg/mL lactate, 0.56 mg/mL NAD+, 1.69 U/mL diaphorase, 0.004% (w/v) BSA, 0.15% (w/v) sucrose, and 0.5 mM 2-p-iodophenyl-3-nitrophenyl tetrazolium chloride (INT). The enzyme reaction was stopped with 1.78 mg/mL oxymate and the absorbance was measured at 490 nm in a 96-well plate reader (Tecan GENios Pro). Potential effects on cell viability were estimated as percentage of extracellular LDH activity. The cytotoxic natural product digitonin (100 μg/mL) was used as a positive control.
Statistical analysis was performed by ANOVA/Bonferroni test (GraphPad PRISM software, version 4).
Figshare: Effect of capsaicin on vascular smooth muscle cell proliferation: raw data. doi: 10.6084/m9.figshare.12897217
RL, DG, VMD and AGA conceived the study. RL, EHH and AGA designed and analyzed the experiments. RL performed the experiments. RL and AGA prepared the first draft of the manuscript. All authors critically commented on and revised the draft manuscript and agreed to the final content.
This work was supported by the EU-FP7 Marie Curie Fellowship 252881, and by the University of Vienna “Back-to-Research Grant” (both to R. Liu); as well as by the Austrian Science Fund (FWF): S10704, P25971-B23, and P23317-B11.
I confirm that the funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
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Competing Interests: No competing interests were disclosed.
Competing Interests: No competing interests were disclosed.
Competing Interests: No competing interests were disclosed.
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http://inpst.net/two-23-year-phd-student-positions-in-drug-discovery-targeting-cardiovascular-diseases-atherosclerosis-at-ighz-pas-poland/
We would ... Continue reading Thanks a lot to the readers for the interest in our work. You might also find of interest the following thematically related open PhD positions:
http://inpst.net/two-23-year-phd-student-positions-in-drug-discovery-targeting-cardiovascular-diseases-atherosclerosis-at-ighz-pas-poland/
We would be especially grateful if you could forward this information to potentially interested PhD candidates.
With best wishes,
Atanas
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International Natural Product Sciences Taskforce
Website: http://inpst.net/
Twitter: https://twitter.com/_INPST
Facebook: https://www.facebook.com/INPST/
ResearchGate: https://www.researchgate.net/project/International-Natural-Product-Science-Taskforce-INPST
http://inpst.net/two-23-year-phd-student-positions-in-drug-discovery-targeting-cardiovascular-diseases-atherosclerosis-at-ighz-pas-poland/
We would be especially grateful if you could forward this information to potentially interested PhD candidates.
With best wishes,
Atanas
---
International Natural Product Sciences Taskforce
Website: http://inpst.net/
Twitter: https://twitter.com/_INPST
Facebook: https://www.facebook.com/INPST/
ResearchGate: https://www.researchgate.net/project/International-Natural-Product-Science-Taskforce-INPST