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Research Article
Revised

Effect of sex in the MRMT-1 model of cancer-induced bone pain

[version 3; peer review: 2 approved]
Sarah Falk1, Tamara Al-Dihaissy1, Laura Mezzanotte2, Anne-Marie Heegaard1
Sarah Falk1, Tamara Al-Dihaissy1, Laura Mezzanotte2, Anne-Marie Heegaard1
PUBLISHED 16 Nov 2015
Author details Author details
OPEN PEER REVIEW
REVIEWER STATUS

Abstract

An overwhelming amount of evidence demonstrates sex-induced variation in pain processing, and has thus increased the focus on sex as an essential parameter for optimization of in vivo models in pain research. Mammary cancer cells are often used to model metastatic bone pain in vivo, and are commonly used in both males and females. Here we demonstrate that compared to male rats, female rats have an increased capacity for recovery following inoculation of MRMT-1 mammary cells, thus potentially causing a sex-dependent bias in interpretation of the data.

Keywords

Cancer-induced bone pain, sex differences, in vivo model, x-ray, pain behavior, bioluminescence

Corresponding author: Sarah Falk
Competing interests: No competing interests were disclosed.
Grant information: The author(s) declared that no grants were involved in supporting this work.
Copyright:  © 2015 Falk S et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Data associated with the article are available under the terms of the Creative Commons Zero "No rights reserved" data waiver (CC0 1.0 Public domain dedication).
How to cite: Falk S, Al-Dihaissy T, Mezzanotte L and Heegaard AM. Effect of sex in the MRMT-1 model of cancer-induced bone pain [version 3; peer review: 2 approved]. F1000Research 2015, 4:445 (https://doi.org/10.12688/f1000research.6827.3) First published: 31 Jul 2015, 4:445 (https://doi.org/10.12688/f1000research.6827.1) Latest published: 16 Nov 2015, 4:445 (https://doi.org/10.12688/f1000research.6827.3)

Revised Amendments from Version 2

Further elaboration on the main results was added to the discussion. In addition, a section on estrogen-receptor status was added.

See the authors' detailed response to the review by Juan M Jiménez-Andrade
See the authors' detailed response to the review by Svetlana Komarova

Introduction

A crucial step in translational research is development of animal models that can accurately mimic the human condition in relation to both symptoms and underlying mechanisms1,2. To understand the molecular mechanism of complex human disease, and thereby create disease-specific treatment, the models need to be reproducible and with a high predictive value, requiring a detailed knowledge of the expected variation in the models used. Cancer-induced bone pain is a highly complex pain state involving cancer cells, bone cells, immune cells as well as neuronal and non-neuronal processing in the periphery and at spinal and supraspinal levels3. Numerous studies have reported on variation in the more than 45 animal models that have been used to model the pain state4. The most common variations are related to cell lines, species or injection site used4, and in addition we have previously demonstrated that sex does also affect the model5, thereby emphasizing the need to consider sex bias.

The majority of preclinical pain research is still performed using male animals, despite an overwhelming amount of both human and animal data demonstrating significant sex variations6–8. Conversely, within the field of bone cancer pain, preclinical researchers have had more focus on the issue, likely due to a more intuitive choice of sex, based on the origin of the cancer cells used. A meta-analysis of studies on cancer-induced bone pain demonstrated that 49% of the studies used males, 22% used females and 29% did not report the sex of the animals used4. These numbers are indeed more balanced than the numbers previously reported, revealing that 79% of all animal studies published in PAIN from 1996 to 2005 were using male animals, whereas only 8% used females8. However, in this study we demonstrate that not only is sex an integral factor to be considered when choosing an animal model, intrinsic sex-dependent variation has to be carefully analyzed in order to avoid bias. The data presented in this study suggest, that in the MRMT-1-Luc2 carcinoma cell model of metastatic bone cancer pain, a sex-dependent bias related to recovery is skewing the behavioral responses observed in females to a greater extent compared to males, potentially masking effects in studies where female animals are used to model the pain state.

Methods

Experiments were performed on 10 male and 10 female Sprague-Dawley rats (Taconic M&B, Denmark) group-housed in cages under a 12-h alternating light/dark cycle with ad libitum access to food and water. In addition, unpublished data from four previous independent in-house experiments were analyzed; these included a total of 16 females and 42 males, and were performed as described in this method section (see Figure 4C). The only difference with respect to methods is that three experiments were performed prior to transfection of the MRMT-1 cells line, and this study and one of the prior experiments were conducted after transfection of the MRMT-1 cells line. For all animals bodyweight and general health was monitored throughout the studies. All experiments were approved by the Danish Animal Experiments Inspectorate under the Danish Ministry of Food, Agriculture and Fisheries (2014-15-0201-00031), and were carried out in accordance to the guidelines of the Committee for Research and Ethical Issues of the International Association for the Study of Pain9.

Generation of MRMT1-Luc2

Generation of MRMT1 cells expressing reporter proteins was achieved by genomic integration of the Luc2-copGFP reporter gene construct using lentiviral transduction. The lentiviral vector contains the EF1α promoter sequence driving the equimolar expression of a codon-optimized firefly luciferase (Luc2) gene and a copepod green fluorescent protein (copGFP) thanks to the presence of a T2A sequence. Vector production and cell transduction were performed under appropriate biosafety level conditions (ML-II) in accordance with the National Biosafety Guidelines and Regulations for Research on Genetically Modified Organisms. Procedures and protocols were reviewed and approved by the LUMC Biosafety Committee (GMO permit 08-129). Vector production and transduction procedures have been described in detail elsewhere10. In brief, cells were seeded in 24-well plate at a cell density of 7.5×104 cells/well and maintained in a humidified incubator at 37°C and 5% CO2. After attachment was accomplished, the cells were transduced using a MOI (multiplicity of infection) of 1. Subsequently cells were FACS sorted for high expression of copGFP and cell culture was expanded for experiments.

Cell culture

Syngeneic MRMT1-Luc2 rat mammary gland carcinoma cells (Leiden University medical Center, The Nederlands) were cultured in RPMI 1640 medium without phenol red (Invitrogen, Paisley, UK/Nærum, Denmark) supplemented with 10% heat-inactivated foetal bovine serum, 1% L-glutamine and 2% penicillin/streptomycin (Invitrogen, Paisley, UK/Nærum, Denmark). On the day of surgery, MRMT1-Luc2 carcinoma cells were released by brief exposure to 0.1% w/v trypsin-EDTA and centrifuged in medium for 3 min at 1200rpm. The pellet was washed twice with Hanks’ balanced salt solution (HBSS) without calcium, magnesium or phenol red (Invitrogen, Paisley, UK/Nærum, Denmark) and centrifuged for 3 min at 1200rpm. Cells were re-suspended in HBSS and kept on ice until use.

Surgery

The inoculation of carcinoma cells was performed modified from 11 as previously described12. Hence, following induction of isoflurane anaesthesia (induction 4%, maintenance 1.5–2%) the animal was placed with the abdominal side up. A small incision was made in a shaved and disinfected area on the anterior-medial surface of the tibia and the tibia carefully exposed. A hole was made in the tibia with a 0,7mm dental drill through which a thin polyethylene tube was fed 1cm into the proximal intramedullary cavity. Animals were injected with 5×103 MRMT1-Luc2 carcinoma cells in 10µl HBSS. Following removal of the tube, the hole was plugged with bone restorative material (IRM, Dentsply, Surrey, UK/Vallensbæk, Denmark). The wound was irrigated with saline and closed with two metal clips. The animals were placed under a heat source until fully awake. Postoperative analgesia was administrated by injection of Rimadyl (s.c. 5mg/kg, Pfizer, Denmark) and application of 2% Lidocaine gel (AstraZeneca, Denmark) to the wound.

Behavioral tests

Behavioral responses were assessed prior to surgery and on day 7, 10, 14, 17, 21 and 23. All animals were introduced to all behavioral tests 2–3 times prior to the start of the experiment. Animals reaching humane endpoint before day 23 (n=7) (predefined as limb use score 0) were euthanized by decapitation following brief exposure to isoflurane, and the behavioral scores from the last day carried forward for data analysis.

Von Frey test. Mechanical hypersensitivity, detected by von Frey filaments (North Coast Medicinal, Inc., Camino Arroyo, Gilroy, CA, USA), was used as an indicator of early pain behavior. The threshold was determined by the up and down method, as described previously13. Briefly, rats were placed in acrylic enclosures on a wire mesh floor and allowed to acclimatize for minimum 30 min. Starting at 6 g, filaments ranging from 0.4–26 g were applied to the plantar surface of the hind paw with a minimum of 3 min intervals between two stimulations. A stimulus was recorded as positive if paw withdrawal was observed within 3 s of stimulation with a given filament; if no response was observed it was recorded as negative. Following a positive response, the next stimulation was performed with a filament with a decreased bending strength, whereas a negative response was followed with stimulation with a filament with increased bending strength. According to the original protocol, optimal threshold calculation by this method requires 6 responses in the immediate vicinity of the 50% threshold, therefore recording of the 6 data points did not begin until the response threshold was first crossed (positive response changes to negative response or inverse). The 2 responses detecting the threshold were designated as the first 2 responses of the series of 6. In cases where continuous negative responses were observed to the maximum of the stimulus set, a value of 26 g was set as the cut-off and used as withdrawal threshold for data analysis. In all other cases the resulting pattern of positive and negative responses was calculated into a 50% response withdrawal threshold using the formula: 50% g threshold = (10Xf+kδ)/(10,000), where Xf = value (log unit) of the final von Frey hair used; k = tabular value for the pattern of positive/negative responses; and δ = mean difference (in log units) between filaments.

Weight-bearing test. Rats were placed in the incapacitance tester (MJS Technology Ltd., Buntingford, Herfordshire, UK) and allowed to acclimatize until calm. Measurements were performed over a period of 4 s and in triplicates. An average weight-bearing ratio was subsequently calculated as the amount of weight placed on the cancer-bearing leg divided by the total amount of weight placed on both legs and used for data analysis.

Limb use test. Rats were allowed to move freely in a transparent plastic cage without bedding (500 mm × 300 mm × 500 mm). Following 10 min of acclimation, each animal was observed for 3 min and assigned a limb use score from 3 to 0 as follows: 3: Normal use of leg, 2: mild or insignificant limping, 1: significant limping, 0: significant limping and part lack of use.

Bioluminescence imaging

Animals were anesthetized in an induction chamber with 2.5% isoflurane (Isobar Vet; 100%, Nomeco, Copenhagen, DK). D-Luciferin (PerkinElmer, Skovlunde, Denmark) dissolved in PBS was administered by intraperitoneal injection (40mg/kg). 10 min after injection, animals were placed on their back in a nose cone in a Lumina XR instrument (Caliper Life Sciences, Teralfene, Belgium) and anesthesia was maintained with a 2.5% isoflurane/oxygen mix. Image capture was performed with binning: M(4), F/stop: 1 and exposure time from 1 s to 60 s according to the power signal. The signal was adjusted according to the exposure time prior to data analysis. For each animal, an average of three images was used for analysis. Between each capture, the animal was repositioned to minimize bias caused by placement of the animals in the machine. Bioluminescence images were analyzed using IVIS Imaging Software (Living Image©, version 4.0.0.9801; Caliper Life Sciences, Teralfene, Belgium). For each image, the threshold was adjusted to 35% of the signal, and the readout was measured in total flux, photos/s.

X-ray analysis

X-Ray images were captured subsequent to the bioluminescence images. The severity of bone degradation was analyzed using ImageJ (ImageJ 1.47v). Each X-Ray image was calibrated to a standard aluminum wedge. The mean grayscale value of a standard region of interest within the trabecular bone of the proximal tibia was measured and the average of two corresponding background regions in the soft tissue proximate to tibia was subtracted. The grayscale value was translated into millimeter aluminum equivalents (mmAl) according to the standard wedge and used as estimate of the relative bone density of the distal femur. Data analysis was blinded for sex of the animals.

Determination of active state

Animals with no bioluminescence signal or lack of osteolysis, hence no active cancer growth, were excluded from the active state group. All animals included in this study were assigned to an active or restored state based on presence of bioluminescence signal and osteolysis (active) or lack of bioluminescence signal and osteolysis (restored). Four previous in-house experiments were reanalyzed with regard to active and restored state based on presence bioluminescence signal and osteolysis or lack of bioluminescence signal and osteolysis, or based on osteolysis or lack of osteolysis alone. One study was similarly using bioluminescence as exclusion criteria, whereas the data from the three previous studies was analyzed with respect to lack of osteolysis. Lack of osteolysis was based on the last measure day, usually day 21–23, whereas one experiment was terminated at day 14, however on this day 10 out of 11 animals showed clear osteolysis.

Adverse events

No adverse events were observed during the experiment.

Statistical analysis

All analyses were blinded for the researchers. Analyses were performed using GraphPad Prism 6, (GraphPad Software, CA, USA), and for all data a 95% confidence interval was used as a measure of statistical significance. All data are expressed as mean ± standard error of mean (S.E.M.). In vitro analysis of bioluminescence signal was analyzed with linear regression. Behavioral, bone degradation and tumor progression data were analyzed using two-way repeated measure ANOVA followed by Bonferroni post-hoc test to compare baseline values to each additional time point, and in addition to compare females to males. Analysis of odds ratio of cancer clearance was tested using Chi-square test. Level of significance for all tests was set at */#p < 0.05, **/##p < 0.01, ***/###p < 0.001, ****/####p < 0.0001.

Results

Validation of bioluminescence emission in cancer cells

The bioluminescence signal was evaluated to validate successful transfection of Luc2 into the MRMT-1 carcinoma cells. In vitro, the signal increased linearly with the number of cells, p <0.0001 (Figure 1A). In vivo, i.p. administration of D-Luciferin induced a robust plateau of the bioluminescence signal 5 min after injection and lasting approximately 20min (Figure 1B). Luc2 was hence successfully transfected into the carcinoma cells, linearly reflecting the number of living cells in vitro, and produced a robust signal in vivo.

b743735d-3341-46ac-babd-4e271dffac41_figure1.gif

Figure 1.

(A) In vitro quantification of bioluminescence signal. Linear regression demonstrated that the signal increased linearly with the number of cells (dotted line), p<0.0001. (B) In vivo quantification. The bioluminescence signal increased during the initial 5min following i.p. injection. After 5min the signal reached a stable plateau lasting for approximately 20min. Results from two experiments.

Sex-dependent variation in disease progression

Pain behavior was quantified by detection of mechanical hypersensitivity, as well as limb use and weight-bearing deficits on days 7, 10, 14, 17, 21 and 23. Compared to baseline level, a significant decline in the 50% withdrawal threshold, reflecting mechanical hypersensitivity, was observed in both females and males from day 10 to 21 (Figure 2A, Dataset 1: rawdata_vonfrey). Limb use scoring was significantly reduced only on day 23 for the females, but on day 17–23 for the males (Figure 2B, Dataset 2: rawdata_limbuse). Decline in weight-bearing ratio was significantly reduced on day 17 and 23 for the females and on day 17–23 for the males (Figure 2C, Dataset 3: rawdata_weight-bearing). No significant difference was observed between the sexes on any of the test days, however females tended to present with a less pronounced limb use and weight-bearing deficit in the later phase of disease progression, days 17–23 (Figure 2B and C).

b743735d-3341-46ac-babd-4e271dffac41_figure2.gif

Figure 2.

(A) A decrease in 50% withdrawal threshold was observed in both females and males as the disease progressed. (B and C) Males demonstrated a significant decrease in limb use and weight-bearing ratio on day 17–23. In contrast, females showed significant decrease limb use only on day 23, and decreased weight-bearing on day 17 and 23. (D) A significant decrease in relative bone density was observed in the males from day 14. A similar, yet insignificant, tendency was observed in the females. */**/***=males compared to baseline, #/##=females compared to baseline.

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This is a portion of the data; to view all the data, please download the file.
Dataset 1.Rawdata_vonfrey.
Animals were placed in acrylic enclosures on a wire mesh floor and allowed to acclimatize for minimum 30 min. Starting at 6 g, filaments ranging from 0.4–26 g were applied to the plantar surface of the hind paw with a minimum of 3 min intervals between two stimulations. A stimulus was recorded as positive if paw withdrawal was observed within 3 s of stimulation with a given filament; if no response was observed it was recorded as negative. Following a positive response, the next stimulation was performed with a filament with a decreased bending strength, whereas a negative response was followed with stimulation with a filament with increased bending strength. The resulting pattern of positive and negative responses was calculated into a 50% response withdrawal threshold using the formula: 50% g threshold = (10Xf+kδ)/(10,000), where Xf = value (log unit) of the final von Frey hair used; k = tabular value for the pattern of positive/negative responses; and δ = mean difference (in log units) between filaments29.
Cage nr.;tail;baseline;7;10;14;17;21;23
A;1;3;3;3;1;0;-;-
;2;3;3;3;3;3;3;3
;3;3;3;3;3;3;3;3
;4;3;3;3;3;3;3;3
;5;3;3;3;3;3;3;0
B;1;3;3;3;3;3;3;2
;2;3;3;3;3;3;3;3
;3;3;3;3;0;0;0;-
;4;3;3;3;3;3;3;3
;5;3;3;3;1;0;-;-
C;1;3;3;3;3;3;3;3
;2;3;3;3;2;2;1;1
;3;3;3;3;0;0;-;-
;4;3;3;3;1;3;3;3
;5;3;3;3;3;3;3;2
D;1;3;3;3;3;0;-;-
;2;3;3;3;3;3;3;3
;3;3;3;3;3;3;3;3
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Dataset 2.Rawdata_limbuse.
Following 10 min of acclimation in a transparent plastic cage, each animal was observed for 3 min and assigned a limb use score from 3 to 0 as follows: 3: Normal use of leg, 2: mild or insignificant limping, 1: significant limping, 0: significant limping and part lack of use30.
Cage A;Day;;;Day;;;Day;;;Day;;;Day;;;Day;;;Day;;;;;;;;;;
Female;Baseline;;;7;;;10;;;14;;;17;;;21;;;23;;;;;;;;;;
1;Left;Right;;Left;Right;;Left;Right;;Left;Right;;Left;Right;;Left;Right;;Left;Right;;;;;;;;;
;3719;3974;;562;5815;;6177;685;;3875;9177;;2289;5696;;;;;;;;;;;;;;;
;4663;5213;;7056;7486;;5303;5963;;4297;9461;;68;9718;;;;;;;;;;;;;;;
;5018;538;;6672;7057;;6115;599;;3869;9493;;552;9618;;;;;;;;;;;;;;;
Ratio;;048;;;049;;;048;;;030;;;014;;;-;;;-;;;;;;;;;
2;Left;Right;;Left;Right;;Left;Right;;Left;Right;;Left;Right;;Left;Right;;Left;Right;;;;;;;;;
;621;6531;;6864;6704;;629;5791;;6237;7411;;3387;803;;7676;7771;;8373;69;;;;;;;;;
;6751;6688;;6558;7582;;6349;5666;;6383;7199;;6686;9638;;8166;8382;;8898;6325;;;;;;;;;
;49;5332;;6608;557;;5993;6848;;6309;6744;;6845;9502;;8248;7474;;8438;11589;;;;;;;;;
Ratio;;049;;;050;;;051;;;047;;;037;;;049;;;048;;;;;;;;;
3;Left;Right;;Left;Right;;Left;Right;;Left;Right;;Left;Right;;Left;Right;;Left;Right;;;;;;;;;
;7699;5434;;6952;6559;;5134;591;;7514;7712;;7339;703;;7522;7591;;3381;6851;;;;;;;;;
;483;7241;;6084;6809;;527;5556;;618;7716;;6749;6687;;8166;8919;;5853;7158;;;;;;;;;
;6832;7539;;6131;6594;;5747;6219;;7209;8299;;7669;7173;;693;6516;;6956;5255;;;;;;;;;
Ratio;;049;;;049;;;048;;;047;;;051;;;050;;;055;;;;;;;;;
4;Left;Right;;Left;Right;;Left;Right;;Left;Right;;Left;Right;;Left;Right;;Left;Right;;;;;;;;;
;5721;5107;;4361;5473;;67;5614;;4516;7658;;6204;5627;;8849;8714;;976;5834;;;;;;;;;
;3849;4934;;4362;4367;;4767;6105;;5245;7122;;7214;7911;;792;726;;5775;8087;;;;;;;;;
;4725;5229;;3633;326;;6234;6236;;5669;8164;;8027;7926;;6056;6838;;7822;7002;;;;;;;;;
Ratio;;048;;;049;;;049;;;040;;;050;;;050;;;048;;;;;;;;;
5;Left;Right;;Left;Right;;Left;Right;;Left;Right;;Left;Right;;Left;Right;;Left;Right;;;;;;;;;
;3263;2659;;7099;591;;7416;7626;;8516;7573;;8428;7339;;6895;7782;;1408;9033;;;;;;;;;
;6753;515;;6456;6105;;7564;8184;;811;7333;;625;9204;;11833;11284;;175;9326;;;;;;;;;
;5455;5652;;6456;5791;;9248;8257;;8336;7255;;6591;7386;;7789;8241;;298;9105;;;;;;;;;
Ratio;;054;;;053;;;050;;;053;;;047;;;051;;;094;;;;;;;;;
;;;;;;;;;;;;;;;;;;;;;;;;;;;;;
;;;;;;;;;;;;;;;;;;;;;;;;;;;;;
Cage B;Day;;;Day;;;Day;;;Day;;;Day;;;Day;;;Day;;;;;;;;;;
Female;Baseline;;;7;;;10;;;14;;;Baseline;;;Baseline;;;Baseline;;;;;;;;;;
1;Left;Right;;Left;Right;;Left;Right;;Left;Right;;Left;Right;;Left;Right;;Left;Right;;;;;;;;;
;7036;7734;;5904;703;;699;6711;;6077;7353;;7649;8915;;8466;8295;;626;11733;;;;;;;;;
;6943;4902;;7885;6936;;7426;7004;;8162;6916;;773;9539;;7258;8048;;8009;12316;;;;;;;;;
;6953;6834;;7924;8532;;6811;7661;;7804;7515;;10331;8486;;6769;7149;;4914;9882;;;;;;;;;
Ratio;;052;;;049;;;050;;;050;;;049;;;051;;;064;;;;;;;;;
2;Left;Right;;Left;Right;;Left;Right;;Left;Right;;Left;Right;;Left;Right;;Left;Right;;;;;;;;;
;5155;5041;;6047;54;;5095;5518;;5744;4543;;4023;7763;;5624;8639;;5638;4495;;;;;;;;;
;5216;4647;;6786;597;;5954;5088;;6943;6326;;5468;585;;8341;6139;;3899;5983;;;;;;;;;
;5507;539;;5152;5974;;5626;7238;;7012;6313;;7834;8077;;5771;4798;;4305;4534;;;;;;;;;
Ratio;;051;;;051;;;049;;;054;;;044;;;049;;;052;;;;;;;;;
3;Left;Right;;Left;Right;;Left;Right;;Left;Right;;Left;Right;;Left;Right;;Left;Right;;;;;;;;;
;7808;6892;;6938;6605;;7207;6758;;1071;6598;;;;;;;;;;;;;;;;;;
;5886;6391;;6552;5956;;8287;6759;;1037;917;;;;;;;;;;;;;;;;;;
;5158;5991;;6071;6817;;3861;5704;;2075;11527;;;;;;;;;;;;;;;;;;
Ratio;;049;;;050;;;049;;;013;;;-;;;-;;;-;;;;;;;;;
4;Left;Right;;Left;Right;;Left;Right;;Left;Right;;Left;Right;;Left;Right;;Left;Right;;;;;;;;;
;7895;6741;;4603;5038;;3903;4909;;316;9324;;4046;5426;;7493;663;;7041;10284;;;;;;;;;
;7267;7069;;5696;5061;;4221;3359;;4999;6574;;7732;5582;;7791;6348;;9773;11322;;;;;;;;;
;5334;608;;;;;;;;6059;6262;;;;;8902;8278;;12363;7152;;;;;;;;;
Ratio;;050;;;050;;;050;;;039;;;050;;;047;;;050;;;;;;;;;
5;Left;Right;;Left;Right;;Left;Right;;Left;Right;;Left;Right;;Left;Right;;Left;Right;;;;;;;;;
;4906;5125;;7061;6891;;635;6766;;2615;7657;;3272;9699;;;;;;;;;;;;;;;
;5236;5721;;7584;7994;;5161;6922;;2541;10394;;2252;10957;;;;;;;;;;;;;;;
;5972;5614;;5108;4398;;5872;4868;;5747;8979;;3514;12248;;;;;;;;;;;;;;;
Ratio;;049;;;051;;;049;;;028;;;022;;;-;;;-;;;;;;;;;
;;;;;;;;;;;;;;;;;;;;;;;;;;;;;
;;;;;;;;;;;;;;;;;;;;;;;;;;;;;
Cage C;Day;;;Day;;;Day;;;Day;;;Day;;;Day;;;Day;;;;;;;;;;
Male;Baseline;;;7;;;10;;;14;;;Baseline;;;Baseline;;;Baseline;;;;;;;;;;
1;Left;Right;;Left;Right;;Left;Right;;Left;Right;;Left;Right;;Left;Right;;Left;Right;;;;;;;;;
;4673;4158;;8936;8003;;9247;10056;;6778;687;;891;9444;;8335;9512;;9141;9718;;;;;;;;;
;5101;5004;;7889;8271;;9216;9532;;9345;7561;;8262;815;;10492;11789;;11131;13221;;;;;;;;;
;589;6005;;6226;7603;;8411;891;;7361;8434;;8993;10445;;13156;11874;;13303;10679;;;;;;;;;
Ratio;;051;;;049;;;049;;;051;;;048;;;049;;;050;;;;;;;;;
2;Left;Right;;Left;Right;;Left;Right;;Left;Right;;Left;Right;;Left;Right;;Left;Right;;;;;;;;;
;5704;5865;;9899;9779;;10636;12092;;7989;7409;;6994;16356;;11216;14805;;5687;13339;;;;;;;;;
;4978;5464;;10339;10124;;8543;9357;;6174;8331;;6687;12993;;9096;1726;;8876;18909;;;;;;;;;
;4843;4317;;9522;9369;;8772;10312;;7116;10552;;1032;12199;;4498;18266;;5429;13852;;;;;;;;;
Ratio;;050;;;050;;;047;;;045;;;037;;;032;;;030;;;;;;;;;
3;Left;Right;;Left;Right;;Left;Right;;Left;Right;;Left;Right;;Left;Right;;Left;Right;;;;;;;;;
;6712;7678;;5328;662;;9813;882;;3191;8412;;;;;;;;;;;;;;;;;;
;7972;6516;;6737;6501;;10088;991;;4239;8955;;;;;;;;;;;;;;;;;;
;7313;6614;;8184;8653;;79;9287;;5238;11395;;;;;;;;;;;;;;;;;;
Ratio;;051;;;048;;;050;;;030;;;-;;;-;;;-;;;;;;;;;
4;Left;Right;;Left;Right;;Left;Right;;Left;Right;;Left;Right;;Left;Right;;Left;Right;;;;;;;;;
;6569;6951;;4442;499;;6868;8439;;3026;10101;;56;8159;;784;4051;;10327;9351;;;;;;;;;
;6853;7653;;8143;7756;;7152;9182;;697;992;;7287;9621;;11512;10867;;12337;14951;;;;;;;;;
;7179;6125;;9619;855;;9394;7859;;9253;12103;;8706;9059;;9061;8931;;9131;9099;;;;;;;;;
Ratio;;050;;;050;;;048;;;036;;;044;;;056;;;049;;;;;;;;;
5;Left;Right;;Left;Right;;Left;Right;;Left;Right;;Left;Right;;Left;Right;;Left;Right;;;;;;;;;
;6426;5924;;8204;8959;;7526;7498;;6756;8199;;3731;8491;;8885;10152;;8974;6599;;;;;;;;;
;8054;7256;;7602;7606;;9755;7523;;8782;10234;;6165;10963;;6679;13067;;2459;13975;;;;;;;;;
;7692;7765;;6867;696;;9122;6904;;9999;9732;;4956;10056;;3346;12513;;9323;5318;;;;;;;;;
Ratio;;051;;;049;;;054;;;047;;;033;;;034;;;045;;;;;;;;;
;;;;;;;;;;;;;;;;;;;;;;;;;;;;;
;;;;;;;;;;;;;;;;;;;;;;;;;;;;;
Cage D;Day;;;Day;;;Day;;;Day;;;Day;;;Day;;;Day;;;;;;;;;;
Male;Baseline;;;7;;;10;;;14;;;16-17;;;Baseline;;;Baseline;;;;;;;;;;
1;Left;Right;;Left;Right;;Left;Right;;Left;Right;;Left;Right;;Left;Right;;Left;Right;;;;;;;;;
;8521;8377;;8641;8182;;7919;8979;;8288;9745;;3356;10417;;;;;;;;;;;;;;;
;9304;8942;;7949;8363;;7052;8965;;8739;10681;;1688;11874;;;;;;;;;;;;;;;
;8928;9297;;8771;9243;;7744;7514;;9776;8216;;1286;15291;;;;;;;;;;;;;;;
Ratio;;050;;;050;;;047;;;048;;;015;;;-;;;-;;;;;;;;;
2;Left;Right;;Left;Right;;Left;Right;;Left;Right;;Left;Right;;Left;Right;;Left;Right;;;;;;;;;
;6951;7253;;933;9405;;6985;5089;;1027;8915;;7908;7707;;9163;1231;;708;14497;;;;;;;;;
;5984;613;;9738;9147;;6721;6754;;9912;9833;;9385;94;;16652;15711;;15221;9404;;;;;;;;;
;6829;6286;;;;;8139;10794;;9761;9858;;8174;9626;;14659;11663;;16441;19495;;;;;;;;;
Ratio;;050;;;051;;;050;;;051;;;049;;;050;;;047;;;;;;;;;
3;Left;Right;;Left;Right;;Left;Right;;Left;Right;;Left;Right;;Left;Right;;Left;Right;;;;;;;;;
;8375;8068;;9077;8317;;6412;7383;;9572;9339;;9135;5528;;12463;11383;;13297;6349;;;;;;;;;
;6685;5303;;7199;7291;;7866;7855;;9626;9079;;1011;7002;;12171;8889;;11589;15054;;;;;;;;;
;4833;5579;;6603;7314;;6472;7491;;8958;9809;;8534;6018;;9276;13038;;8503;7299;;;;;;;;;
Ratio;;051;;;050;;;048;;;050;;;060;;;051;;;055;;;;;;;;;
4;Left;Right;;Left;Right;;Left;Right;;Left;Right;;Left;Right;;Left;Right;;Left;Right;;;;;;;;;
;7204;6639;;7801;922;;6916;6195;;723;6443;;1826;9674;;;;;;;;;;;;;;;
;5023;4836;;9472;8935;;9884;7959;;6134;7667;;94;14858;;;;;;;;;;;;;;;
;5222;4976;;6575;696;;9931;10991;;4989;449;;1639;1381;;;;;;;;;;;;;;;
Ratio;;051;;;049;;;052;;;050;;;011;;;-;;;-;;;;;;;;;
5;Left;Right;;Left;Right;;Left;Right;;Left;Right;;Left;Right;;Left;Right;;Left;Right;;;;;;;;;
;7622;7906;;9615;8121;;879;9792;;3668;8478;;2104;10418;;;;;;;;;;;;;;;
;7627;6057;;8237;7365;;12104;10896;;4777;13568;;964;11937;;;;;;;;;;;;;;;
;7065;7992;;701;9125;;10443;9498;;3846;12307;;958;12074;;;;;;;;;;;;;;;
Dataset 3.Rawdata_weight-bearing.
http://dx.doi.org/10.5256/f1000research.6827.d96689 Animals were placed in the incapacitance tester and allowed to acclimatize until calm. Measurements were performed over a period of 4 s and in triplicates. An average weight-bearing ratio was subsequently calculated as the amount of weight placed on the cancer-bearing leg divided by the total amount of weight placed on both legs and used for data analysis31.

The relative bone density was significantly reduced compared to baseline measures in the males from day 14 and throughout the study (Figure 2D, Dataset 4: rawdata_xray). A similar, however insignificant, tendency was observed in the females (Figure 2D). No difference was found between males and females at any time point. Overall, the data suggest a less severe progression in females compared to males.

Animal;Day;Sex (F=female M=male);Tibia same ROI;Wedge;Soft tissue A;Soft tissue B;Soft mean;Tibia-soft mean;Adjusted to wedge;Total tibia;;;;;;;
A.1;Baseline;F;195.667;46.667;105.841;177.029;141435;54232;046667;25308;;;;;;;
A.2;Baseline;F;234.304;59.188;135.986;208.565;1722755;620285;059188;36713;;;;;;;
A.3;Baseline;F;233.101;52.971;132.130;200.072;166101;67000;052971;35491;;;;;;;
A.4;Baseline;F;221.565;52.130;119.696;202.609;1611525;604125;05213;31493;;;;;;;
A.5;Baseline;F;198.797;43.377;112.768;169.043;1409055;578915;043377;25112;;;;;;;
B.1;Baseline;F;182.116;44.652;101.710;169.536;135623;46493;044652;20760;;;;;;;
B.2;Baseline;F;211.435;45.507;136.217;184.406;1603115;511235;045507;23265;;;;;;;
B.3;Baseline;F;191.826;49.565;116.652;190.130;153391;38435;049565;19050;;;;;;;
B.4;Baseline;F;204.246;49.406;113.768;194.377;1540725;501735;049406;24789;;;;;;;
B.5;Baseline;F;196.420;46.449;112.783;177.058;1449205;514995;046449;23921;;;;;;;
C.1;Baseline;M;208.739;47.594;123.870;187.884;155877;52862;047594;25159;;;;;;;
C.2;Baseline;M;235.304;49.754;133.986;208.536;171261;64043;049754;31864;;;;;;;
C.3;Baseline;M;205.812;46.014;113.319;180.986;1471525;586595;046014;26992;;;;;;;
C.4;Baseline;M;220.855;49.667;131.609;203.304;1674565;533985;049667;26521;;;;;;;
C.5;Baseline;M;222.754;50.725;136.841;205.623;171232;51522;050725;26135;;;;;;;
D.1;Baseline;M;246.043;54.391;145.232;222.391;1838115;622315;054391;33848;;;;;;;
D.2;Baseline;M;229.913;47.058;147.058;201.754;174406;55507;047058;26120;;;;;;;
D.3;Baseline;M;224.464;51.826;129.710;211.681;1706955;537685;051826;27866;;;;;;;
D.4;Baseline;M;193.145;41.667;109.145;166.188;1376665;554785;041667;23116;;;;;;;
D.5;Baseline;M;219.130;49.188;135.783;196.768;1662755;528545;049188;25998;;;;;;;
;;;;;;;;;;;;;;;;;
A.1;7;F;187.246;46.203;101.855;170.159;136007;51239;046203;23674;;;;;;;
A.2;7;F;209.174;52.768;115.826;194.870;155348;53826;052768;28403;;;;;;;
A.3;7;F;203.348;48.696;106.493;182.145;144319;59029;048696;28745;;;;;;;
A.4;7;F;219.275;50.957;112.725;184.681;148703;70572;050957;35961;;;;;;;
A.5;7;F;184.290;47.159;98.870;172.391;1356305;486595;047159;22947;;;;;;;
B.1;7;F;194.391;47.928;103.812;169.232;136522;57869;047928;27735;;;;;;;
B.2;7;F;203.710;47.652;117.174;176.536;146855;56855;047652;27093;;;;;;;
B.3;7;F;183.333;45.058;103.594;174.130;138862;44471;045058;20038;;;;;;;
B.4;7;F;200.638;46.217;105.681;174.159;139920;60718;046217;28062;;;;;;;
B.5;7;F;195.594;44.391;110.130;172.638;141384;54210;044391;24064;;;;;;;
C.1;7;M;208.681;48.855;117.087;193.971;155529;53152;048855;25967;;;;;;;
C.2;7;M;224.203;51.261;145.377;205.565;175471;48732;051261;24981;;;;;;;
C.3;7;M;209.841;48.188;119.594;189.986;154790;55051;048188;26528;;;;;;;
C.4;7;M;206.739;46.783;130.478;191.551;1610145;457245;046783;21391;;;;;;;
C.5;7;M;210.246;47.217;113.609;177.507;145558;64688;047217;30544;;;;;;;
D.1;7;M;191.058;44.580;108.406;175.928;142167;48891;04458;21796;;;;;;;
D.2;7;M;208.362;48.493;123.667;187.507;155587;52775;048493;25592;;;;;;;
D.3;7;M;219.014;51.899;128.652;201.101;1648765;541375;051899;28097;;;;;;;
D.4;7;M;235.638;49.841;127.348;192.899;1601235;755145;049841;37637;;;;;;;
D.5;7;M;232.174;54.406;142.101;211.188;1766445;555295;054406;30211;;;;;;;
;;;;;;;;;;;;;;;;;
A.1;10;F;218.739;56.159;126.464;195.217;1608405;578985;056159;32515;;;;;;;
A.2;10;F;200.913;52.493;101.536;184.029;1427825;581305;052493;30514;;;;;;;
A.3;10;F;202.725;50.058;107.739;181.884;1448115;579135;050058;28990;;;;;;;
A.4;10;F;220.826;54.478;121.464;201.101;1612825;595435;054478;32438;;;;;;;
A.5;10;F;215.507;56.217;124.870;201.928;163399;52108;056217;29294;;;;;;;
B.1;10;F;208.957;54.275;112.971;193.957;153464;55493;054275;30119;;;;;;;
B.2;10;F;206.986;52.957;107.493;194.710;1511015;558845;052957;29595;;;;;;;
B.3;10;F;202.594;53.768;124.406;194.870;159638;42956;053768;23097;;;;;;;
B.4;10;F;218.667;52.667;113.884;197.029;1554565;632105;052667;33291;;;;;;;
B.5;10;F;207.841;51.812;115.928;197.464;156696;51145;051812;26499;;;;;;;
C.1;10;M;214.942;48.391;121.087;192.333;156710;58232;048391;28179;;;;;;;
C.2;10;M;223.145;51.319;141.899;210.667;176283;46862;051319;24049;;;;;;;
C.3;10;M;204.188;49.580;122.696;188.087;1553915;487965;04958;24193;;;;;;;
C.4;10;M;202.942;47.464;115.667;188.855;152261;50681;047464;24055;;;;;;;
C.5;10;M;233.942;51.913;143.304;207.855;1755795;583625;051913;30298;;;;;;;
D.1;10;M;224.536;52.319;128.797;197.739;163268;61268;052319;32055;;;;;;;
D.2;10;M;233.783;48.739;143.275;205.696;1744855;592975;048739;28901;;;;;;;
D.3;10;M;240.884;56.029;143.087;214.652;1788695;620145;056029;34746;;;;;;;
D.4;10;M;227.072;49.884;130.725;195.536;1631305;639415;049884;31897;;;;;;;
D.5;10;M;219.464;49.971;135.087;200.696;1678915;515725;049971;25771;;;;;;;
;;;;;;;;;;;;;;;;;
A.1;14;F;180.014;49.580;100.261;185.667;142964;37050;04958;18369;;;;;;;
A.2;14;F;206.130;46.565;100.652;177.145;1388985;672315;046565;31306;;;;;;;
A.3;14;F;212.174;48.290;119.362;196.971;1581665;540075;04829;26080;;;;;;;
A.4;14;F;195.870;45.971;103.145;168.203;135674;60196;045971;27673;;;;;;;
A.5;14;F;184.406;47.101;102.507;173.130;1378185;465875;047101;21943;;;;;;;
B.1;14;F;191.739;48.638;104.739;177.261;141000;50739;048638;24678;;;;;;;
B.2;14;F;185.145;47.464;108.957;181.377;145167;39978;047464;18975;;;;;;;
B.3;14;F;191.725;51.391;122.420;198.029;1602245;315005;051391;16188;;;;;;;
B.4;14;F;191.696;51.304;117.333;194.696;1560145;356815;051304;18306;;;;;;;
B.5;14;F;191.043;48.928;116.348;194.333;1553405;357025;048928;17469;;;;;;;
C.1;14;M;202.609;49.014;129.580;204.812;167196;35413;049014;17357;;;;;;;
C.2;14;M;188.188;47.841;121.348;198.739;1600435;281445;047841;13465;;;;;;;
C.3;14;M;189.072;49.464;129.710;197.319;1635145;255575;049464;12642;;;;;;;
C.4;14;M;185.043;49.681;118.797;195.739;157268;27775;049681;13799;;;;;;;
C.5;14;M;199.986;52.275;121.029;208.362;1646955;352905;052275;18448;;;;;;;
D.1;14;M;185.580;46.957;122.275;188.014;1551445;304355;046957;14292;;;;;;;
D.2;14;M;201.812;45.812;135.826;185.072;160449;41363;045812;18949;;;;;;;
D.3;14;M;232.217;51.333;140.319;209.768;1750435;571735;051333;29349;;;;;;;
D.4;14;M;193.261;48.232;125.406;190.406;157906;35355;048232;17052;;;;;;;
D.5;14;M;204.420;51.739;155.957;202.232;1790945;253255;051739;13103;;;;;;;
;;;;;;;;;;;;;;;;;
;;;;;;;;;;;;;;;;;
A.1;17;F;156.768;46.145;110.145;171.725;140935;15833;046145;7306;;;;;;;
A.2;17;F;200.609;47.536;99.058;176.623;1378405;627685;047536;29838;;;;;;;
A.3;17;F;188.348;46.319;98.565;167.522;1330435;553045;046319;25616;;;;;;;
A.4;17;F;193.261;49.333;107.739;180.043;143891;49370;049333;24356;;;;;;;
A.5;17;F;180.986;50.594;114.319;187.464;1508915;300945;050594;15226;;;;;;;
B.1;17;F;194.087;49.565;116.464;185.319;1508915;431955;049565;21410;;;;;;;
B.2;17;F;188.754;46.174;110.725;175.696;1432105;455435;046174;21029;;;;;;;
B.3;17;;;;;;;;;;;;;;;;
B.4;17;F;180.623;46.594;116.333;170.652;1434925;371305;046594;17301;;;;;;;
B.5;17;F;198.536;47.768;123.391;184.203;153797;44739;047768;21371;;;;;;;
C.1;17;M;206.377;54.826;125.739;208.797;167268;39109;054826;21442;;;;;;;
C.2;17;M;201.188;51.971;140.768;208.797;1747825;264055;051971;13723;;;;;;;
C.3;17;;;;;;;;;;;;;;;;
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Dataset 4.Rawdata_xray.
The severity of bone degradation was analysed. The x-ray was calibrated to a standard aluminium wedge. The mean grayscale value of a standard region of interest within the trabecular bone of the proximal tibia was measured and the average of two corresponding background regions in the soft tissue proximate to tibia was subtracted. The grayscale value was translated into millimeter aluminium equivalents (mmAl) according to the standard wedge and used as estimate of the relative bone density of the distal femur32.

Females have increased capacity for recovery

All animals had a detectable bioluminescent signal on days 7 and 10, hence demonstrating successful inoculation of living cancer cells (Figure 3, Dataset 5: rawdata_BLI, Dataset 6: rawdata_bioluminescence). From day 14 an increasing number of animals lost the bioluminescence signal, suggesting recovery in a subset of animals. Compared to females with consistent bioluminescence signal, a subset of females losing bioluminescence signal displayed a significant decrease in the signal from day 17 (Figure 3). A similar but later occurring tendency was observed in the males (Figure 3).

b743735d-3341-46ac-babd-4e271dffac41_figure3.gif

Figure 3. Bioluminescence (BLI) signal reflecting progression of tumor growth.

The signal increases in both males and females during the initial 14 days, and reaches a plateau for the remaining of the study. From day 14 an increasing numbers of animals lost the bioluminescence signal. ##/###=females without signal compared to females with signal.

Loss of bioluminescence signal was observed in 40% of the females, but only in 20% of the males (Figure 4A and B). A significant trend for loss of signal was observed in both females and males, p=0.0079 and p=0.0353 respectively. A similar pattern in recovery was observed in previous independent in-house experiments. In these experiments, lack of osteolysis (determined by x-ray analysis) was used as an exclusion criterion. In one study, 5 out of 16 female animals displayed a lack of osteolysis (Figure 4C)14. Three additional experiments using male animals demonstrated lack of osteolysis at the end of the experiment in 3 out of 21, 1 out of 10 and 1 out of 11 animals, respectively12 (Supplementary figure S1 and Supplementary figure S2). Pooling the data from the five experiments demonstrated a significant difference in the odds ratio between females and males, reflecting a significantly greater proportion of females clearing active cancer growth compared to males, as indicated by loss of bioluminescence signal and/or lack of cancer-induced osteolysis, p=0.029, (Figure 4D).

b743735d-3341-46ac-babd-4e271dffac41_figure4.gif

Figure 4.

(A and B) Cumulative numbers of females and males losing bioluminescence signal over time. (C) Numbers of animals with restored states in five independent in-house experiments. The recovery is based on either loss of bioluminescence signal or lack of osteolysis. (D) A significant difference is found between the odds ratio of recovering in females and males. *=females compared to males.

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Dataset 5.Rawdata_BLI.
Images were obtained 10min after i.p. injection of 40mg/kg Luciferase. Image capture was performed with binning: M(4), F/stop: 1 and exposure time from 1 s to 60 s according to the power signal. For each animal, an average of three images was used for analysis. For each image, the threshold was adjusted to 35% of the signal, and the readout was measured in total flux, photos/s33.
Dataset 6.Rawdata_bioluminescence.
Representative bio-luminescence images34.

Loss of bioluminescence signal is associated with reversal of disease progression

Despite the presence of living cancer cells during the initial 10 days, neither pain behavior nor relative bone density was significantly changed in animals losing bioluminescence signal (Figure 5A–D). A transient but insignificant decrease in the 50% withdrawal threshold was observed in animals later losing bioluminescent signal (Figure 5A, rawdata_vonfrey). In addition, a slight decrease in limb use, weight-bearing and relative bone density was observed on day 14, however the decrease was insignificant and returned to baseline levels on the next measured day (Figure 5B–D, rawdata_limbuse, rawdata_weight-bearing, rawdata_xray). Overall this suggests initial normal disease progression, followed by subsequent recovery and hence normal relative bone density and lack of pain behavior. In addition, a strong correlation between bioluminescence signal and relative bone density was observed (Figure 6A) supporting the division of animals into two groups; animals with a clear bioluminescence signal and osteolysis, indicating active cancer growth (Figure 6A, B and C) and animals with no bioluminescence signal and no osteolysis, indicating recovery (Figure 6A, E and D).

b743735d-3341-46ac-babd-4e271dffac41_figure5.gif

Figure 5. Pain-related behavior and relative bone density in animal losing bioluminescence signal during the experiment.

(A) A transient, yet insignificant, decrease was observed in the 50% threshold in females. (B, C) Limb use and weight-bearing deficit was not observed in either females or males during the experiment. (D) The relative bone density remained the same throughout the experiment in both males and females.

b743735d-3341-46ac-babd-4e271dffac41_figure6.gif

Figure 6. Correlation between relative bone density and bioluminescence signal.

(A) Animals without bioluminescence signal all had high relative bone density (blue), whereas animals with high bioluminescence signal demonstrated low relative bone density (black). Examples of osteolytic (C) and non-osteolytic bone (D). Examples of high bioluminescence signal (B) and lack of signal (E).

Sex-difference screws progression of pain behavior

Exclusion of animals successfully recovering aligned the onset and severity of pain behavior in both sexes (Figure 7A–C). In addition, a similar effect was seen on the relative bone density, reflecting similar bone degradation in both females and males with active growing cancer (Figure 5D). No significant difference was observed between females and males at any time point.

b743735d-3341-46ac-babd-4e271dffac41_figure7.gif

Figure 7. Pain-related behavior and relative bone density in animals with a consistent bioluminescence signal throughout the experiment.

(A, B and C) Both females and males demonstrate significant decreases in 50% withdrawal threshold, limb use score and weight-bearing ration. (D) Both sexes show a similar extent of bone degradation. **/***/****=males compared to baseline, #/##/####=females compared to baseline.

Discussion

To increase the translational potential of preclinical research, it is essential to continually focus on refining and optimizing the animal models used. Increased focus has been given to sex as a variable, initially driven by the contradictive issue that preclinical research has been biased towards use of male animals despite the fact the chronic pain is highly overrepresented in women8. Although females are in most cases likely the most intuitive sex for models of metastatic breast cancer, such as the MRMT-1 model of cancer-induced bone pain, our data suggest that care should be taken when interpreting the data. Our data demonstrates that the females have an increased capacity for recovering from the disease state, reflected by loss of bioluminescence signal accompanied by normal limb use and weight-bearing ratio as well as normal relative bone density throughout the study.

The increased odds ratio for recovery in the females introduces a potential bias in interpretation of the data. Importantly, although there is no significant difference between females and male, the increased recovery in the females pulls the mean of the females’ readouts towards baseline to a much greater degree than the males, thereby increasing the risk of underestimating the severity of the disease in the females. In order to get a reliable model truly reflecting the human disease, it is critical to exclude the animals capable of recovery, as these animals are not reflecting the pain state observed in patients. This means that it is highly important to include a parameter for discrimination between an “active disease state” and a “recovered from disease state”. Inclusion of animals with recovered states clearly mask the severity of the disease in the females, as limb use is only significantly decreased on the last day and the relative bone density is not significantly affected at any time point (Figure 2A and D). However, excluding females with recovered disease state reveals that females are in fact severely affected at an earlier time point, demonstrated by an significant decrease in limb use already on day 17 and in addition significant decreased relative bone density from day 21 (Figure 7B and D).

In addition, our data demonstrates that bioluminescence signal is a reliable measure for the active disease state. All animals with continuous bioluminescence signal subsequently developed pain-related behavior and decreased relative bone density. In contrast, animals losing bioluminescence signal during the experiment did not develop pain-related behavior and demonstrated normal relative bone density. This is in agreement with other studies demonstrating that bioluminescence signal is predictable measure for quantification of cancer cells in vivo15,16. To further validate the recovery state, histology could be made to confirm that loss of bioluminescence signal is actually caused by clearance of the cancer cells and not just loss of signal from the cancer cells. However, since the first model of cancer-induced bone pain was developed, bone degradation has been demonstrated to associate with tumor burden numerous times17–21. In this study, the loss of bioluminescence signal is accompanied by lack of bone lesions and in addition lack of pain behaviors making it highly unlikely that there is still active cancer growth in the animals.

Importantly, this study emphasizes the need to consider sex-dependent variation beyond simple disease readouts. The data demonstrate that there are not significant difference between females and males with respect to pain behavior and bone degradation. However, there are sex-differences in the model which needs to be carefully considered when modeling cancer-induced bone pain in females, as the increased recovery rate in the females can potentially mask the progression. The specific mechanisms underlying the sex-dependent difference in recovery are currently not known, and requires an extensive investigation of hormonal and cellular responses. One potential cause could be sex-dependent differences in the immune responses. It is now generally accepted that inherent properties and influence of sex hormones induce more potent immune and inflammatory reactions in females compared to males22,23. An intuitive cause could therefore be, that the females’ increased recovery rate is linked to an increased immune response to inoculation with the cancer cells possible modulated by sex hormones. It should be noted that the capacity for recovering was seen regardless of whether the cancer cells expressed luciferase or not, and is thus not due to an immune reaction to the luciferase enzyme. However, as the MRMT-1 are estrogen receptor-postive20, the relationship might not be so simple. Estrogen generally cause increase growth in estrogen receptor-positive cells24, suggesting that the female immune system would have to fight a more rapidly growing tumor, which would suggest a lower recovery rate. A full understand of the involvement of sex hormones on the progression of MRMT-1 requires extensive studies; however our data suggest a minor role in the overall progression of cancer-induced bone pain in this model. This is also supported by the similar progression in males and female rats despite the estrogen receptor-positives status of the cells.

In addition, recent work from Sorge et al. demonstrate that different cells mediates pain hypersensitivity in female and male mice25. They found that although the males and females developed the same degree of mechanical allodynia following nerve injury, there was a fundamental difference in the cellular involvement. Whereas microglia activity was required for mechanical pain hypersensitivity in males, this was not the case in females, where the hypersensitivity was driven by adaptive immune cells, likely T lymphocytes. Our data suggest that a similar phenomenon could be involved in progression of MRMT-1 induced cancer pain. In agreement with Sorge et al., we find a similar pain response in females and males, however since there is a significant difference in the recovery rate between the sexes, it might indicate the different systems are mediating the pain state in female and male rats.

Another factor potentially affecting the sex-dependent effect might be the local microenvironment in the bone. The microenvironment around the tumor might be different due to nonspecific sex-differences, hence potentially affecting the growth of the tumor cells following inoculation26–28. Interestingly, the observed difference is likely species or cell line dependent. We have previously reported that in a similar model of cancer-induced bone pain, based on 4T1-luc2 mammary cancer cell inoculation in femur of BALB/cJ mice, females have a significantly greater initial bioluminescence signal compared to males. The females had, in addition, an earlier onset of pain behavior, but a similar bone degradation rate5. This suggests that in the mouse model, intrinsic sex-dependent variation favors more aggressive progression in females compared to males, whereas the rat model displays better odds ratio for recovery in the females.

Data availability

F1000Research: Dataset 1. Rawdata_vonfrey, 10.5256/f1000research.6827.d9668729

F1000Research: Dataset 2. Rawdata_limbuse, 10.5256/f1000research.6827.d9668830

F1000Research: Dataset 3. Rawdata_weight-bearing, 10.5256/f1000research.6827.d9668931

F1000Research: Dataset 4. Rawdata_xray, 10.5256/f1000research.6827.d9669032

F1000Research: Dataset 5. Rawdata_BLI, 10.5256/f1000research.6827.d9669133

F1000Research: Dataset 6. Rawdata_bioluminescence, 10.5256/f1000research.6827.d9669234

Author contributions

S.F. conceived and designed the study and carried out all experimental procedures. T.A-D. assisted during experimental procedures. L.M. generated the MRMT-1-Luc2 cell line. A-M.H. provided advice and facilities. S.F. wrote the manuscript that was edited and approved by all coauthors.

Competing interests

No competing interests were disclosed.

Grant information

The author(s) declared that no grants were involved in supporting this work.

Supplementary Material

b743735d-3341-46ac-babd-4e271dffac41_suppl_figure1.gif

Supplementary figure S1.

Representative images of animals demonstrating osteolysis (A–F) and lack of osteolysis (G,H).

b743735d-3341-46ac-babd-4e271dffac41_suppl_figure2.gif

Supplementary figure S2.

Representatives of animals demonstrating bioluminescence signal (A–C, E,F) and lack of bioluminescence signal (D).

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Falk S, Al-Dihaissy T, Mezzanotte L and Heegaard AM. Effect of sex in the MRMT-1 model of cancer-induced bone pain [version 3; peer review: 2 approved]. F1000Research 2015, 4:445 (https://doi.org/10.12688/f1000research.6827.3)
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Approved with reservations A number of small changes, sometimes more significant revisions are required to address specific details and improve the papers academic merit.
Not approvedFundamental flaws in the paper seriously undermine the findings and conclusions
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Reviewer Report 22 Dec 2015
Juan M Jiménez-Andrade, Unidad Académica Multidisciplinaria Reynosa-Aztlán, Universidad Autónoma de Tamaulipa​s (UAT), Reynosa, Mexico 
Approved
VIEWS 13
https://doi.org/10.5256/f1000research.7974.r11695
The authors have properly ... Continue reading
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Jiménez-Andrade JM. Reviewer Report For: Effect of sex in the MRMT-1 model of cancer-induced bone pain [version 3; peer review: 2 approved]. F1000Research 2015, 4:445 (https://doi.org/10.5256/f1000research.7974.r11695)
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Reviewer Report 30 Nov 2015
Svetlana Komarova, Faculty of Dentistry, McGill University, Montreal, QC, Canada 
Approved
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https://doi.org/10.5256/f1000research.7974.r11231
The revisions fully addressed ... Continue reading
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Komarova S. Reviewer Report For: Effect of sex in the MRMT-1 model of cancer-induced bone pain [version 3; peer review: 2 approved]. F1000Research 2015, 4:445 (https://doi.org/10.5256/f1000research.7974.r11231)
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Svetlana Komarova, Faculty of Dentistry, McGill University, Montreal, QC, Canada 
Approved with Reservations
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https://doi.org/10.5256/f1000research.7621.r10387
I read the paper with interest and, although I do not always agree with the authors’ interpretation of the data, I found the study thought-provoking and analysis thorough and complex.
  1. In my view, the main result of the study is that
... Continue reading
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HOW TO CITE THIS REPORT
Komarova S. Reviewer Report For: Effect of sex in the MRMT-1 model of cancer-induced bone pain [version 3; peer review: 2 approved]. F1000Research 2015, 4:445 (https://doi.org/10.5256/f1000research.7621.r10387)
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  • Author Response 16 Nov 2015
    Sarah Falk, Department of Drug Design and Pharmacology, University of Copenhagen, Copenhagen, 2100, Denmark
    16 Nov 2015
    Author Response
    We thank Dr. Komarova for the helpful comments, and acknowledge that the study is somewhat untraditional in the sense that the main focus is on the increased variation observed in ... Continue reading
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  • Reviewer Response 30 Nov 2015
    Komarova Svetlana, Faculty of Dentistry, McGill University, Montreal, Canada
    30 Nov 2015
    Reviewer Response
    Thank you for your response. I believe that new discussion provides a significant and novel focus for the study.
    Competing Interests: No competing interests were disclosed.
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  • Author Response 16 Nov 2015
    Sarah Falk, Department of Drug Design and Pharmacology, University of Copenhagen, Copenhagen, 2100, Denmark
    16 Nov 2015
    Author Response
    We thank Dr. Komarova for the helpful comments, and acknowledge that the study is somewhat untraditional in the sense that the main focus is on the increased variation observed in ... Continue reading
    Report a concern
  • Reviewer Response 30 Nov 2015
    Komarova Svetlana, Faculty of Dentistry, McGill University, Montreal, Canada
    30 Nov 2015
    Reviewer Response
    Thank you for your response. I believe that new discussion provides a significant and novel focus for the study.
    Competing Interests: No competing interests were disclosed.
    Report a concern
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Reviewer Report 20 Aug 2015
Juan M Jiménez-Andrade, Unidad Académica Multidisciplinaria Reynosa-Aztlán, Universidad Autónoma de Tamaulipa​s (UAT), Reynosa, Mexico 
Approved with Reservations
VIEWS 36
https://doi.org/10.5256/f1000research.7341.r10035
This is very interesting and well presented study; however the data does not support the title and conclusions at all of the present study. For this reviewer it is unclear what is the functional implication of females having an increased ... Continue reading
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Jiménez-Andrade JM. Reviewer Report For: Effect of sex in the MRMT-1 model of cancer-induced bone pain [version 3; peer review: 2 approved]. F1000Research 2015, 4:445 (https://doi.org/10.5256/f1000research.7341.r10035)
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Report a concern
  • Author Response 18 Sep 2015
    Sarah Falk, Department of Drug Design and Pharmacology, University of Copenhagen, Copenhagen, 2100, Denmark
    18 Sep 2015
    Author Response
    Dear Professor Jiménez-Andrade
     
    Thank you for your very constructive review. We have taken your comments into carefully consideration, and made the following changes:
     
    We have changed the title to “Effect of sex ... Continue reading
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COMMENTS ON THIS REPORT
  • Author Response 18 Sep 2015
    Sarah Falk, Department of Drug Design and Pharmacology, University of Copenhagen, Copenhagen, 2100, Denmark
    18 Sep 2015
    Author Response
    Dear Professor Jiménez-Andrade
     
    Thank you for your very constructive review. We have taken your comments into carefully consideration, and made the following changes:
     
    We have changed the title to “Effect of sex ... Continue reading
    Report a concern

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Approved
The paper is scientifically sound in its current form and only minor, if any, improvements are suggested
Approved with reservations
A number of small changes, sometimes more significant revisions are required to address specific details and improve the papers academic merit.
Not approved
Fundamental flaws in the paper seriously undermine the findings and conclusions

Reviewer Reports

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Version 1
31 Jul 15 		read
  1. Juan M Jiménez-Andrade, Universidad Autónoma de Tamaulipa​s (UAT), Reynosa, Mexico
  2. Svetlana Komarova, McGill University, Montreal, Canada

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    Alongside their report, reviewers assign a status to the article:
    Approved - the paper is scientifically sound in its current form and only minor, if any, improvements are suggested
    Approved with reservations - A number of small changes, sometimes more significant revisions are required to address specific details and improve the papers academic merit.
    Not approved - fundamental flaws in the paper seriously undermine the findings and conclusions
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