Keywords
Schiff base, L-tryptophan, copper (II) complex, synthesis, cytotoxicity, HeLa, KCL-22
Schiff base, L-tryptophan, copper (II) complex, synthesis, cytotoxicity, HeLa, KCL-22
Schiff bases are considered as a very important class of organic ligands having a wide range of applications in many fields of biomedicine1–3. They are the condensation products of an amino compound with an active carbonyl compound and carry imine or azomethine (–C=N–) functional group, which is essential for their biological activity. Structurally, Schiff base is a nitrogen analog of an aldehyde or ketone, in which the carbonyl group (C=O) has been replaced by an imine or azomethine group. While aliphatic aldehyde containing Schiff bases are unstable in nature and readily get polymerized, aromatic aldehyde containing compounds are more stable due to conjugation system4.
Schiff bases derived from aromatic aldehydes and aromatic amines are widely applicable in the fields of biology, inorganic and analytical chemistry5,6. Their biological activities are based on the earlier detected anti-inflammatory, antiviral, antibacterial, antifungal, antimalarial and antipyretic properties7–10. The bonding interactions between aromatic amino acid side chains of the receptor and aromatic/heteroaromatic rings of the ligand were revealed in most of X-ray crystal structures of protein complexes with small molecules. This protein-ligand recognition, based on aromatic ring involved non-covalent interactions, can ensure the application of Schiff bases, derived from aromatic aldehydes and amines, in drug design process11–13. Moreover, the evaluation of the structure-activity relationship of Schiff bases, derived from different substituted aromatic amines and aldehydes, demonstrated the importance of the latter for desired biological activity14–15.
Pyridinecarboxaldehyde derivatives of Schiff bases are of great interest because of their role in natural and synthetic organic chemistry. It is known, that pyridoxal-amino acid systems are important in numerous metabolic reactions intermediated with amino acid and pyridoxal. So far, pyridinecarboxaldehyde isomers characterized by different localization of carboxaldehyde group (2-, 3- or 4-) relative to nitrogen atom in pyridine ring are valuable precursors for complex forming Schiff bases, since they can exhibit physiological effects similar to pyridoxal-amino acid systems. Thus, the pyridinecarboxaldehydes containing Schiff bases are expected to have enhanced biological activities.
It is known that the binding of bioorganic molecules or drugs to metal ions drastically change their biomimetic properties, therapeutic effects, and pharmacological properties16. Schiff base derivatives of aromatic amino acids are good chelating agents and capable to form stable complexes with transition metals and exhibit significant biological and enzymatic activities17,18. The most widely studied cation in this respect is copper, which is implicated in a wide range of vital cell functions. Copper has been proven to be beneficial against several diseases such as tuberculosis, rheumatoid, gastric ulcers and cancers19–21. Many non-toxic, lipid-soluble, small molecular mass copper chelate complexes have been shown to have superoxide dismutase- and catalase-like activities22–24, which makes them essential for de novo syntheses of metalloelement-dependent enzymes required for oxygen utilization and prevention of oxygen superoxide accumulation.
The present study describes the synthesis and characterization of isomeric 2-, 3- and 4-pyridinecarboxaldehydes and L-tryptophan derived Schiff bases and their copper (II) complexes, as well as the assessment of their cytotoxic activity.
All chemicals and solvents used were of analytical grade. The reagents used for the synthesis of the Schiff bases and their copper (II) complexes were obtained from Sigma-Aldrich (Sigma-Aldrich Co. LLC, USA), including L-tryptophan, 2-; 3-; and 4-pyridinecarboxaldehydes, KOH, copper (II) acetate, and methanol.
Schiff bases (2pyr.Trp, 3pyr.Trp, and 4pyr.Trp) were synthesized by condensation of potassium salt of L-tryptophan and isomeric (2-, 3-, 4-) pyridinecarboxaldehydes, respectively, in alcohol solutions (ethanol, methanol) in 5°C–25°C temperature range and 1:1 molar ratio. First, 10 mM of L-tryptophan was dissolved in 100 mL of alcohol solution, containing KOH (10mM) by permanent stirring under dry nitrogen at 18°C–20°C. Then 10 mM of the corresponding isomer of pyridincarboxyaldehyde (2-, 3- or 4-) was added to the resulting solution with stirring and refluxed at 50°C for 2 hours resulting in a yellow colored solution that indicates the Schiff base formation. The volume of the solution was then reduced in vacuo using a rotary evaporator. Anhydrous ether was added to deposit a yellowish precipitate, which was then re-crystallized from alcohol.
The obtained Schiff bases were served as ligands for the synthesis of appropriate copper (II) complexes (namely, Cu-2pyr.Trp, Cu-3pyr.Trp, and Cu-4pyr.Trp). The synthesis was performed at 20±2°С in alcohol media (methanol, ethanol) using potassium hydroxide and copper acetate. Complex formation was carried out in a reaction medium without preliminary isolation of Schiff bases. Compound isolation was performed by partial evaporation of the solvent, settling, centrifugation, re-crystallization, and vacuum drying.
For characterization of Schiff bases and their copper (II) complexes the infrared (IR) absorbance spectra were obtained in the range of 4000–400 cm-1s in Vaseline oil on KBr plates using Spectrometer IR 75 (Carl Zeiss, Jena). For assessment of thermal stability of obtained compounds the IR absorbance spectra were recorded every 30 minutes in the 60°С – 100оС temperature range for 2 hours.
The elemental analysis was performed by combustion in a pure oxygen environment using PerkinElmer 2400 Series II CHNS/O Elemental Analyzer (PerkinElmer, USA). The nuclear magnetic resonance (NMR) spectra were obtained in D2O and CD3OD on Spectrometer Varian 300 MHz (Agilent, USA).
Solubility assessment of synthesized compounds was carried out according to standard test method protocol25. Schiff bases were water-soluble, while their copper (II) complexes were soluble in dimethyl sulfoxide (DMSO). Stock solutions of Schiff bases and their copper (II) complexes at the concentration of 10 mM/mL were prepared and diluted with nutrition medium RPMI-1640 or DMEM. Only freshly prepared solutions were used in experiments.
Human HeLa (cervix carcinoma) and KCL-22 (chronic myeloid leukemia) cell lines were obtained from the cell culture collection of the Institute of Molecular Biology (Yerevan, Armenia). Growth media (DMEM and RPMI-1640) as well as media supplements were obtained from Sigma-Aldrich. The human HeLa and KCL-22 cell lines were routinely maintained at 37°C in the growth medium DMEM (HeLa) and RPMI-1640 (KCL-22), supplemented with 10% fetal bovine serum (HyClone, UK), 2 mM L-glutamine (Sigma Aldrich, Germany), 100 IU/mL penicillin (Sigma Aldrich, Germany) and 100 μg/mL streptomycin (Sigma Aldrich, Germany).
The cytotoxicity of test compounds was assessed using standard protocols for Trypan blue exclusion test and neutral red uptake (NRU) assay26,27.
Trypan blue exclusion test: The KCL-22 cells were seeded into 15 ml glass vials at the density of 0.5 × 106 cells/mL. After 48 hours the test compounds were added at the concentrations of 0.1 µM/mL, 1 µM/mL, 10 µM/mL, 100 µM/mL, and 1000 µM/mL. After further incubation for 48 hours, cells were stained with 0.4% Trypan blue solution for 5-15 minutes and counted in a haemocytometer under a light microscope. The viable cell number was determined.
NRU assay: The HeLa cells were seeded at the density of 0.3 × 106 cells/mL into 96-well plates (Corning, USA), incubated for 48 hours, and then test compounds were added to the cell cultures at the concentrations of 0.1 µM/mL, 1 µM/mL, 10 µM/mL, 100 µM/mL, and 1000 µM/mL. After further incubation for 48 hours the NRU assay was performed. The absorbance was measured using a microplate reader (Human Reader HS, Germany) at a wavelength of 570 nm.
Cell viability was expressed as a percentage of the negative control (cell cultures with no treatment). Doses inducing 50% inhibition of cell viability (the IC50 value) were calculated to determine the cytotoxicity of Schiff bases and their copper(II) complexes.
The extrapolation of obtained IC50 values into LD50 values for compound acute rodent oral toxicity in vivo was performed. The regression formula was used to weigh up the starting doses for single oral application in rats28:
Based on obtained LD50 values the class of toxicity was assigned to all synthesized compounds29. Since the human cell lines were used for the experiments, the IC50 values obtained have also prognostic significance for human30.
All experiments were done in at least three replicates. At least triplicate cultures were scored for an experimental point. All values were expressed as means ± SE. The Student’s one tailed t-test was applied for statistical analysis of results, p < 0.05 was considered as the statistically significant.
The optimal conditions of the Schiff base synthesis with the use of potassium salt of L-tryptophan were identified, allowing to obtain the yield of target products up to 85% for 2pyr.Trp, 75% for 3pyr.Trp, and 80% for 4pyr.Trp. Based on 1H NMR spectra inspection for synthesized Schiff bases in D2O and CD3OD, the loss of 2 singlet signals of СН2 group was revealed, confirming the presence of target compounds. The observed 1Н NMR spectral data, particularly, the presence of CH signal (duplet-duplets) of –СН2–СН– in the range of 4.1–4.5 ppm are characteristic for Schiff bases. The results of elemental analysis of 2pyr.Trp, 3pyr.Trp and 4pyr.Trp Schiff bases are presented in the Table 1.
The brutto chemical formula for 2pyr.Trp, 3pyr.Trpand 4pyr.Trp Schiff bases was identified as C17H14N3O2K (Mr=331.41). The suggested structure of synthesized Schiff bases is presented in the Figure 1. Decomposition temperature of 2pyr.Trp, 3pyr.Trp, and 4pyr.Trp Schiff bases was in the range of 180ºC – 190ºC.
Obtained Schiff bases were then used for the synthesis of corresponding copper (II) complexes (Cu-2pyr.Trp, Cu-3pyr.Trp, Cu-4pyr.Trp). Based on IR analysis the shift of IR absorbance bands upon formation of copper metallocomplexes with Schiff bases was observed (Table 2). Upon formation of Schiff bases the valence deviation (NH) of the tryptophan indole ring was shifted from 3430cm-1 to 3180-3190cm-1 due to the intramolecular interaction of indole and pyridine rings. In copper metallocomplexes this band appeared in the area of 3250–3270cm-1, which was associated with the changes in conjugation linkage degree (C=N) with pyridine ring caused by coordination bond Cu....N. This in turn resulted in changes of interactions between the pyridine and indole rings. Coordination bond Cu....N caused also a shift of valence deviations (C=N) towards low frequencies, and this band was practically overlapped by the band of valence deviations of (C=О-). The results of the determination of the copper content in metallocomplexes by atomic absorption and elemental analysis of carbon, nitrogen and hydrogen are presented in the Table 3. The obtained data allowed to suggest that metallocomplexes contain two Schiff base ligands and have brutto formula C34H28N6O4Cu (Mr = 648.17). The inferred structures of Cu-2pyr.Trp, Cu-3pyr.Trp, Cu-4pyr.Trp metallocomplexes are presented in the Figure 2. Thermostability assessment demonstrated no changes in IR spectra at 100оС during 2 hours. Decomposition temperature for these compounds was at a range of 180оC – 190оC without melting.
Suggested structure of Cu-2pyr.Trp (A), Cu-3pyr.Trp (B), Cu-4pyr.Trp (C) metallocomplexes.
The synthesized Schiff bases and their copper complexes were tested in vitro to determine their cytotoxicity in Hela and KCL-22 cell lines. Our results indicate that the cytotoxic activity of 2pyr.Trp and its copper (II) complex depends on a cell line (Figure 3, A and B, Dataset 1, File 1). In case of 2pyr.Trp action in HeLa cell line, a hormesis effect was apparent, since a significant increase in the cell number was observed at lower concentrations of 0.1-1µM/mL. Further dosage increase, however, did not lead to the total cell death, since the cell viability was more than 90% compared to untreated cells, even at the highest concentration tested (1000 µM/mL). In contrast, the KCL-22 cell line was more sensitive against cytotoxicity of 2pyr.Trp; the hormesis effect was only slightly visible, but the further dose-dependent cell viability decrease was observed and resulting in 20% of cell viability at the highest tested concentration (1000 µM/mL) (Figure 3, A). In case of Cu-2pyr.Trp the sensitivity of cell lines was reversed. Starting from 10 µM/mL concentration the viability of HeLa cells decreased substantially compared with the KCL-22 cell line (Figure 3, B).
The cytotoxicity of 2pyr.Trp (A) and Cu-2pyr.Trp (B) in HeLa and KCL-22 cell lines. Dose-response curves were obtained after 48 hours of treatment with Schiff base 2pyr.Trp and its copper(II) complex Cu-2pyr.Trp at the concentration range of 0.1–1000 µM/mL. Cell viability was expressed as a percentage of the negative control (cell cultures with no treatment). Doses inducing 50% inhibition of cell viability (the IC50 value) were calculated to determine the cytotoxicity of 2pyr.Trp and Cu-2pyr.Trp. The IC50 value estimated for 2pyr.Trp in KCL-22 cell line was equal to 56±9.1 μM/mL, whereas the viability of HeLa cells was more than 90% at the highest concentration tested (A). The IC50 values estimated for Cu-2pyr.Trp were equal to 7±1.7 μM/mL and 80±7.5 μM/mL for HeLa and KCL-22 cell lines, respectively (B).
The non-cytotoxic profile was observed for Schiff base 3pyr.Trp in both cell lines (Figure 4, A, Dataset 1, File 2), while, Cu-3pyr.Trp demonstrated the increased cytotoxic activity against both cell lines. (Figure 4, B). However, the IC50 value was possible to estimate only for HeLa cells, since the viability of KCL-22 cells was more than 60% at the highest concentration tested (1000 µM/mL).
The cytotoxicity of 3pyr.Trp (A) and Cu-3pyr.Trp (B) in HeLa and KCL-22 cell lines. Dose-response curves were obtained after 48 hours of treatment with Schiff base 3pyr.Trp and its copper(II) complex Cu-3pyr.Trp at the concentration range of 0.1–1000 µM/mL. Cell viability was expressed as a percentage of the negative control (cell cultures with no treatment). Doses inducing 50% inhibition of cell viability (the IC50 value) were calculated to determine the cytotoxicity of 3pyr.Trp and Cu-3pyr.Trp. The non-cytotoxic profile was observed for 3pyr.Trp in both cell lines, since the viability of HeLa and KCL-22 cells was around 100% at the highest concentration tested (A). The Cu-3pyr.Trp demonstrated the increased cytotoxic activity against both cell lines, however, the IC50 value was possible to estimate only for HeLa cells (500±5.6 μM/mL), since the viability of KCL-22 cells was more than 60% at the highest concentration tested (B).
The toxicity profile of 4pyr.Trp (Figure 5, A and B, Dataset 1, F3) was similar to 2pyr.Trp, since the slight hormesis effect was again evident in HeLa cell line, while the KCL-22 cells were more sensitive against its cytotoxic activity (Figure 5, A). Despite these similarities, the overall cytotoxic activity of 4pyr.Trp was higher compared to 2pyr.Trp. The level of cell viability at the highest tested concentration (1000 µM/mL) for 4pyr.Trp (Figure 5, A) were 70% for HeLa and 12% for KCL-22, respectively, while in case of 2pyr.Trp viability levels were 90% (HeLa) and 30% (KCL-22), respectively (Figure 4, A). Again, HeLa cells were more susceptible to the cytotoxicity of Cu-4pyr.Trp than KCL-22 cells (Figure 5, B). Earlier, several Schiff bases were tested in vitro for their cytotoxic activity against different cell lines and the structure activity relationship of compounds was discussed17,31,32. Furthermore, Kril et al. reported on the hormesis effect for MCF-7 and 647-V tumour cells, and suggested that receptor-mediated mechanisms are responsible for the observed phenomenon. Here, we also demonstrated the hormesis effect in HeLa cell line, which supports the statement about potential receptor-binding ability of Schiff bases due to the presence of carbon–nitrogen double bond. The changes in cytotoxic activity against cancer cell lines were shown earlier depending on the presence of different groups (chloro, methoxy, nitro, and phenyl) in aromatic rings of a Schiff base molecule32. Furthermore, we have noted that even the localization of carboxaldehyde group at 2-, 3- or 4-position with regard to nitrogen of aromatic ring can affect the cytotoxicity of Schiff bases.
The cytotoxicity of 4pyr.Trp (A) and Cu-4pyr.Trp (B) in HeLa and KCL-22 cell lines. Dose-response curves were obtained after 48 hours of treatment with Schiff base 4pyr.Trp and its copper(II) complex Cu-4pyr.Trp at the concentration range of 0.1–1000 µM/mL. Cell viability was expressed as a percentage of the negative control (cell cultures with no treatment). Doses inducing 50% inhibition of cell viability (the IC50 value) were calculated to determine the cytotoxicity of 4pyr.Trp and Cu-4pyr.Trp. The IC50 value estimated for 4pyr.Trp in KCL-22 cell line was equal to 100±6.5 μM/mL, whereas the viability of HeLa cells was more than 60% at the highest concentration tested (A). The IC50 values estimated for Cu-4pyr.Trp were equal to 10±5 μM/mL and 30±3.8 μM/mL for HeLa and KCL-22 cell lines, respectively (B).
Based on dose-response curves the half-maximal inhibitory concentrations (IC50 values) were estimated for Schiff bases and their copper (II) complexes (Table 4). Our data suggest that Schiff bases 2pyr.Trp, 3pyr.Trp and 4pyr.Trp are non-toxic for HeLa cells since the IC50 values were impossible to estimate even at the highest tested concentration (1000 µM/mL). The 2pyr.Trp (IC50=56±9.1 μM/mL) was two times more toxic against the KCL-22 cell line, than 4pyr.Trp (IC50=100±6.5 μM/mL), while the 3pyr.Trp demonstrated the same non-toxic profile as it was shown in HeLa cells.
The cytotoxic activity was observed for Cu-2pyr.Trp, Cu-3pyr.Trp and Cu-4pyr.Trp in HeLa cell line with the IC50 values of 7±1.7 μM/mL, 500±5.6 μM/mL and 10±5.0 μM/mL, respectively. Those IC50 values were significantly lower in comparison with their copper free analogs. The same tendency was demonstrated for Cu-4pyr.Trp in KCL-22 cell line, where the IC50 value decreased up to 30±3.7 μM/mL. In case of Cu-2pyr.Trp and Cu-3pyr.Trp complexes tested in KCL-22 cell line, no significant differences in IC50 values were observed in comparison with their copper free analogs. Thus, it can be assumed that the cytotoxic activity of Schiff bases tends to increase at complex formation with the copper molecule (Figure 6).
*p<0.01.
Testing of the compounds’ effects on the viability of cells grown in culture is widely used as a predictor of potential toxic effects in whole animals28. Our extrapolated data on the predicted LD50 doses demonstrated that the tested compounds 3pyr.Trp, 4pyr.Trp, and Cu-3pyr.Trp belong to the Class IV of non-toxic chemicals, while 2pyr.Trp, Cu-2pyr.Trp, and Cu-4pyr.Trp belong to the Class III of slightly toxic compounds (Table 4)29. Since the human cell lines were used for the experiments, those hazard classification data have also a prognostic significance for the human. The United States Food and Drug Administration (FDA) states that it is essential to perform toxicological studies during the development of new drugs, since the desirable pharmacological activity needs to be achieved in the absence of acute toxicity33. The non-toxic profile of 3pyr.Trp, 4pyr.Trp and Cu-3pyr.Trp Schiff bases indicates that this compounds can be considered as new entities in drug development process.
We have synthesized and characterized several new Schiff bases of aromatic amino acid derivatives and their copper complexes. Cytotoxicity tests indicated that 3pyr.Trp, 4pyr.Trp, and Cu-3pyr.Trp are non-toxic for human, whereas compounds 2pyr.Trp, Cu-2pyr.Trp, and Cu-4pyr.Trp retain slight toxicity. Moreover, obtained results indicate that cell lines HeLa (epithelial origin) and KCL-22 (derived from blood) vary in sensitivity to the cytotoxic action of the tested compounds; the latter suggests the tissue-/cell line-specificity of their effect. The results also demonstrate that structural alterations, namely, the localization of the carboxaldehyde group at 2-, 3- or 4-position with regard to nitrogen of pyridine ring in aldehyde component of the L-tryptophan derivative Schiff bases and corresponding copper complexes essentially change the biological activity of the compounds tested. The carboxaldehyde group at 2- and 4-positions leads to the higher cytotoxic activity, than that of at 3-position, the presence of the copper in the complexes, mostly increases the cytotoxicity. Thus, the results obtained may be used for the further development of pharmaceutical agents based on L-tryptophan and pyridinecarboxaldehyde derived Schiff bases and their copper(II) complexes.
F1000Research: Dataset 1. raw data of generated dose-response curves, 10.5256/f1000research.9226.d13023534
MM, RA and AA formulated the initial project and aims. VT, DT and VM carried out the synthesis and characterization of the tested compounds. NB contributed to the experimental design of toxicity studies, RG and NS carried out the toxicity studies. MM, NB and AA prepared the first draft of the manuscript. All authors contributed to the review of the manuscript and agreed to the final content.
Authors acknowledge funding from the International Science and Technology Center (ISTC) in the frames of the projects A-1764 (MM) and A-2116 (MM and AA).
The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
We would like to thank Dr. Gennadi Gasparyan for his advice and expertise that greatly assisted the research.
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Competing Interests: No competing interests were disclosed.
Competing Interests: No competing interests were disclosed.
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