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Research Article
Revised

Analysis of a large food chemical database: chemical space, diversity, and complexity

[version 2; peer review: 3 approved]
PUBLISHED 10 Aug 2018
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This article is included in the Cheminformatics gateway.

Abstract

Background: Food chemicals are a cornerstone in the food industry. However, its chemical diversity has been explored on a limited basis, for instance, previous analysis of food-related databases were done up to 2,200 molecules. The goal of this work was to quantify the chemical diversity of chemical compounds stored in FooDB, a database with nearly 24,000 food chemicals.
Methods: The visual representation of the chemical space of FooDB was done with ChemMaps, a novel approach based on the concept of chemical satellites. The large food chemical database was profiled based on physicochemical properties, molecular complexity and scaffold content. The global diversity of FooDB was characterized using Consensus Diversity Plots.
Results: It was found that compounds in FooDB are very diverse in terms of properties and structure, with a large structural complexity. It was also found that one third of the food chemicals are acyclic molecules and ring-containing molecules are mostly monocyclic, with several scaffolds common to natural products in other databases.
Conclusions: To the best of our knowledge, this is the first analysis of the chemical diversity and complexity of FooDB. This study represents a step further to the emerging field of “Food Informatics”. Future study should compare directly the chemical structures of the molecules in FooDB with other compound databases, for instance, drug-like databases and natural products collections. An additional future direction of this work is to use the list of 3,228 polyphenolic compounds identified in this work to enhance the on-going polyphenol-protein interactome studies.

Keywords

ChemMaps, chemical space, chemoinformatics, consensus diversity plots, diversity, FooDB, Foodinformatics, in silico

Revised Amendments from Version 1

We thank the reviewers for the valuable comments and suggestions. We addressed all the comments of Piotr Minkiewicz emphasizing on the novelty, implications and future directions of this work. In the revised version of the manuscript the three suggested references were added and discussed accordingly. It is now mentioned that the findings of this work agree with the results of Lacroix S. et al. and the list of polyphenolic compounds made available in this work can further complement the works of Jensen K. et al. (2014 and 2015). In the revised manuscript we also acknowledged the optional suggestions of Khushbu Shah. The rationale behind the selection of the three version of the data sets was added. It was also acknowledged as a future work, the suggestion of conducting a systematic analysis of the functional groups in the acyclic compounds of FooDB.

See the authors' detailed response to the review by Piotr Minkiewicz
See the authors' detailed response to the review by Rachelle J. Bienstock
See the authors' detailed response to the review by Khushbu Shah

Introduction

Despite the high relevance of food chemicals in many areas including nutrition, disease prevention, and broad impact in the food industry, the chemical space and diversity of food chemical databases (Minkiewicz et al., 2016) has been quantified on a limited basis. Previous efforts include the analysis and comparison of about 2,200 Generally Recognized as Safe (GRAS) flavoring substances (discrete chemical entities only) with compound databases relevant in drug discovery and natural product research e.g., drugs approved for clinical use, compounds in the ZINC database, and natural products from different sources (Burdock & Carabin, 2004; González-Medina et al., 2016; González-Medina et al., 2017; Martinez-Mayorga et al., 2013; Medina-Franco et al., 2012; Peña-Castillo et al., 2018). Other food-related chemical databases, comprising around 900 compounds, were analyzed by Ruddigkeit and J.-L. Reymond (Ruddigkeit & Reymond, 2014). The limited quantitative analysis of food chemicals has been in part due to the scarce availability of food chemical databases in the public domain. A major exception, however, is FooDB a large database with more than 20,000 food chemicals (The Metabolomics Innovation Centre, 2017). To date, it is the most informative public repository of food compounds.

As part of a continued effort to characterize the chemical contents and diversity of food chemicals (González-Medina et al., 2016; Martinez-Mayorga & Medina-Franco, 2009; Medina-Franco et al., 2012), herein we report a quantitative analysis of the chemical space and chemical diversity of FooDB. Widely characterized compound databases such as GRAS, approved drugs and screening compounds used in drug discovery projects were employed as references. We used well-established and novel (but validated) chemoinformatic methods to analyze compound collections. Although most of these approaches are commonly used in drug discovery, this and previous works show they can be readily applied for food chemicals (Peña-Castillo et al., 2018). Thereby this study represents a contribution to further advance the emerging field of Foodinformatics (Martinez-Mayorga & Medina-Franco, 2014).

Methods

Databases and data curation

Four chemical databases were homogeneously curated and analyzed, namely: FooDB version 1.0 (accessed November, 2017) (The Metabolomics Innovation Centre, 2017), drugs approved for clinical use available in DrugBank 5.0.2. (Law et al., 2014), GRAS (Burdock & Carabin, 2004), and a random subset of drug-like natural products from ZINC 12 (Irwin & Shoichet, 2005), of a size comparable to FooDB. The GRAS and DrugBank sets used in this work also have been used as reference in other comparative studies (Medina-Franco et al., 2012). The random set from ZINC was employed just as reference and other random sets from ZINC could be used. Compounds from all databases were washed and prepared using Wash MOE 2017 node in KNIME version 3.5.3 (Berthold et al., 2008). Briefly, the washing protocol implemented in MOE included removing salts and neutralizing the charges in the molecules. The largest fragments were kept and duplicates in each dataset deleted. Table 1 summarizes the databases and sizes after data preprocessing.

Table 1. Compound databases analyzed in this work.

DatabaseSizea
FooDB23,883
GRAS2,244
DrugBank8,748
Natural products in ZINC (drug-like random subset)24,000

aNumber of compounds after data curation

GRAS: Generally Recognized as Safe

Chemical space visualization

The visual representation was generated with ChemMaps, a novel method for large chemical space visualizations (Naveja & Medina-Franco, 2017). Briefly, ChemMaps is able to generate two- and three-dimensional representations of the chemical space based. It uses as input the pairwise chemical similarity computed using fingerprints data. This approach exploits the ‘chemical satellites’ concept (Oprea & Gottfries, 2001), i.e., molecules whose similarity to the rest of the molecules in the database yield sufficient information for generating a visualization of the chemical space. Further details of ChemMaps are described elsewhere (Naveja & Medina-Franco, 2017).

Physicochemical properties

Six physicochemical properties (PCP) were calculated with RDKit KNIME nodes version 3.4, namely: SlogP (partition coefficient), TPSA (topological polar surface area), AMW (atomic mass weight), RB (rotatable bonds), HBD (hydrogen bond donors) and HBA (hydrogen bond acceptors). For the analysis reported in this short communication, these properties were selected based on their broadly extended use for cross-comparison of compound databases of biological relevance. However, additional properties can be calculated.

Molecular complexity

Fraction of sp3 carbons and number of stereocenters were computed for FooDB as measures of structural complexity. Despite the fact that there are several other measures, these two are straightforward to interpret, easy to calculate and are becoming standard to make cross comparisons among databases (Méndez-Lucio & Medina-Franco, 2017). As described in the Results and Discussion section, the computed values for FooDB were compared to literature data already reported for the reference data sets.

Scaffold content

The term “molecular scaffold” is employed to describe the core structure of a molecule (Brown & Jacoby, 2006). Different approaches have been proposed to consistently obtain a molecule’s scaffold in silico. In this work, scaffolds were generated under the Bemis-Murcko definition using the RDKit nodes available in KNIME (Bemis & Murcko, 1996). Bemis and Murcko define a scaffold as “the union of ring systems and linkers in a molecule”, i.e., all side chains of a molecule are removed.

Global diversity

The so-called “global diversity” (or total diversity) of FooDB was assessed and compared to other reference collections using a consensus diversity plot (González-Medina et al., 2016). As described recently, a consensus diversity plot simultaneously represents, in two-dimensions, four diversity criteria: structural (based on pairwise molecular fingerprint similarity values), scaffolds (using Murcko scaffolds computed as described in the Scaffold content section), physicochemical properties (based on the six properties described in Physicochemical properties section), and database size (the number of compounds) (González-Medina et al., 2016). The structural diversity of each data set is represented on the X-axis and was defined as the median Tanimoto coefficient of MACCS keys fingerprints. The scaffold diversity of each database is represented on the Y-axis and was defined as the area under the corresponding scaffold recovery curve, a well-established metric to measure scaffold diversity (Medina-Franco et al., 2009). The diversity based on PCP was defined as the Euclidean distance of six auto-scaled properties (SlogP, TPSA, AMW, RB, HBD, and HBA - vide supra) and is shown as the filling of the data points using a continuous color scale. The relative number of compounds in the data set is represented with a different size of the data points (smaller data sets are represented with smaller data points).

Results and discussion

Visual representation of the chemical space

Chemical space of FooDB in comparison with the compounds of the three reference databases is visualized in Figure 1. The figure also shows the individual comparisons of FooDB with GRAS, DrugBank and natural products subset from ZINC, respectively. As shown in Figure 1a, the coverage of chemical space of FooDB is quite large as compared to other datasets. Most GRAS compounds lie within the chemical space framed by FooDB (Figure 1b): indeed, 1,193 compounds (53% of GRAS) are structurally identical between the two databases. Hence, FooDB largely contains and upgrades structural information from GRAS. There is significant overlap with approved drugs (Figure 1c) and natural products from ZINC with FooDB (Figure 1d).

de1a1bcc-f0b3-474d-b099-f682a7c8013c_figure1.gif

Figure 1. Representation of the chemical space of FooDB.

The visual representation was generated with ChemMaps (Naveja & Medina-Franco, 2017). a) Comparison of FooDB with three reference collections. Panels bd) show comparisons of FooDB with individual data sets.

Distribution of physicochemical properties

Figure 2 shows the boxplots for the distribution of PCP in all the four databases. For better visualization, the outliers above or below the median +/- 1.5 interquartile range are omitted. As expected, due to the large structural diversity, distribution of PCP in FooDB is broad, in many cases overcoming even approved drugs. For most properties, except RB, several compounds in FooDB share the properties of drugs, and drug-like natural products in ZINC. The comparable physicochemical properties between compounds from FooDB and DrugBank encourages additional systematic investigations for bioactivity of food components. Of course, during this search one needs to consider that compounds with similar properties may have different activity profile. In turn, GRAS consists mostly of small-sized compounds. Table S1 (Supplementary File 1) summarizes the statistics for FooDB and other reference collections.

de1a1bcc-f0b3-474d-b099-f682a7c8013c_figure2.gif

Figure 2. Distribution of physicochemical properties.

Box plots of the distribution of six physicochemical properties of FooDB and reference data sets. SlogP (partition coefficient), TPSA (topological polar surface area), AMW (atomic mass weight), RB (rotatable bonds), HBD (hydrogen bond donors) and HBA (hydrogen bond acceptors).

Molecular complexity

For FooDB, the fraction of sp3 carbons (mean: 0.62; standard deviation: 0.28) and the number of stereocenters (mean: 4.7; standard deviation: 7.1) indicated a high structural complexity. For comparison, it has reported that the mean of the fraction of sp3 carbons for approved drugs, compounds in the clinic and a general screening collections of organic compounds is 0.47, 0.41 and 0.32, respectively (González-Medina et al., 2016; Lovering et al., 2009). Moreover, the reported mean of the fraction of sp3 carbons for natural products collections ranges between 0.41 and 0.58 (for natural products in ZINC and Traditional Chinese Medicine (López-Vallejo et al., 2012). The complexity of compounds in FooDB is comparable to molecules in GRAS (mean: 0.63; standard deviation: 0.28) (González-Medina et al., 2016).

Scaffold content

Figure 3 shows the frequency of the most common scaffolds in FooDB. Many compounds are acyclic (32%), followed by monocyclic compounds with a benzene (6%), cyclohexene (2%) and tetrahydropyran (1%) as a core structure. The benzene ring is the most common core scaffold in chemical databases used in drug discovery (Bemis & Murcko, 1996; Singh et al., 2009; Yongye et al., 2012). Many of the most frequent scaffolds in FooDB are also common in other compound databases of natural products (González-Medina et al., 2017). In a follow-up work, it will be interesting to explore the type of functional groups commonly present in the acyclic structures of FooDB.

de1a1bcc-f0b3-474d-b099-f682a7c8013c_figure3.gif

Figure 3. Frequency of the ten most common scaffolds in FooDB.

Recently, Schneider et al. published an analysis on the selectivity of Bemis-Murcko scaffolds based on public bioactivity data available in ChEMBL (Schneider & Schneider, 2017). 78 of the 585 scaffolds reported therein were present in FooDB. The list of the 78 matching scaffolds, along with the original statistics calculated by Schneider et al., is made available as Dataset 1 (Naveja et al., 2018a). Of note, the three most frequent scaffolds in FooDB (benzene, cyclohexane and tetrahydropyran, with more than 300 compounds - Figure 3) are matching scaffolds. Interestingly, the mean Information content (I) value of all 585 Schneider’s scaffolds is 2.8 (sd= 0.6), while the subset of the 78 scaffolds also present in FooDB has a mean I value of only 2.1 (sd = 0.7). Lower I values point towards more promiscuous scaffolds (Schneider & Schneider, 2017), an expected finding given the nature of the database. As example, Table S2 (Supplementary File 1) shows and discusses briefly the statistics for the three most frequent matching scaffolds.

Polyphenols. Since polyphenols are an important class of compounds in food chemistry (Rasouli et al., 2017), we investigated and quantified the amount of polyphenols in FooDB. Polyphenols are well-known antioxidants, which may play a role in the prevention of several diseases including type 2 diabetes, cardiovascular diseases, and some types of cancer (Neveu et al., 2010). In this line, it is known that oxidative/nitrosative stress has a pivotal role in pathophysiology of neurodegenerative disorders and other kinds of disease (Ebrahimi & Schluesener, 2012). Polyphenols have been demonstrated to elicit several biological effects in in vitro and ex vivo tests (Del Rio et al., 2010; Scalbert et al., 2005).

The molecular structure of polyphenols includes at least two phenolic groups, or one biphenol, and up to any additional number of OH substitutions in aryl rings. They may be classified by their structure in two major groups: flavonoids and non-flavonoids (phenolic acid derivatives) (Del Rio et al., 2013). Some polyphenols, such as quercetin, are found in all plant products, whereas others are specific to particular foods. In many cases, food contain complex mixtures of polyphenols, which are often poorly characterized (Manach et al., 2004).

Polyphenols are also a common chemical motif among natural products, and they are often associated to promiscuity (Tang, 2016). In this work it was found that 3,228 (13.5%) compounds in FooDB are polyphenolic. The list of all 3,228 polyphenolic compounds is made available as Dataset 2 (Naveja et al., 2018b). This set of polyphenols is larger than the 502 polyphenols from food indexed in Phenol-Explorer (Neveu et al., 2010). For comparison, all the reference databases used in this work contained less polyphenols than FooDB. GRAS, ZINC and DrugBank contained 15 (0.6%), 24 (0.1%) and 325 (3.7%) polyphenols, respectively. The large list of polyphenols identified from FooDB is larger than the list of 1,395 polyphenols identified and used in the recent work of Lacroix et al. (Lacroix et al., 2018) that was retrieved from Phenol-Explorer and the Dictionary of Natural Products. Indeed, the list of 3,228 polyphenolic compound made available in this work can be used to augment the already extensive polyphenol-protein interactome work of Lacroix et al. (Lacroix et al., 2018).

Global diversity

Since the diversity of compound data sets depend on the molecular representation (Sheridan & Kearsley, 2002), a global assessment of the diversity of FooDB was analyzed using different criteria: molecular fingerprints, scaffolds, physicochemical properties and number of compounds. The four criteria were analyzed in an integrated manner through a Consensus Diversity Plot generated as described in the Global diversity section of the Methods. The Consensus Diversity Plot in Figure 4 shows that FooDB has about average diversity both by fingerprints and relatively low diversity by scaffolds. Although PCP (represented with the color of the data points) are extremely diverse, structural motifs seem to reappear with slight variations. Figure 4 shows the overall large fingerprint and scaffold diversity of approved drugs (e.g., data points towards the lower left region of the plot). Similarly, the relative global diversity of GRAS i.e., high fingerprint diversity but low scaffold diversity (e.g., upper left region of the plot), is consistent with previous comparisons of these compounds with other reference data sets (González-Medina et al., 2016; Medina-Franco et al., 2012).

de1a1bcc-f0b3-474d-b099-f682a7c8013c_figure4.gif

Figure 4. Consensus Diversity Plot of FooDB and reference data sets.

The structural diversity of each data set is represented on the X-axis and was defined as the median Tanimoto coefficient of MACCS keys fingerprints. The scaffold diversity of each database is represented on the Y-axis and was defined as the area under the corresponding scaffold recovery curve. The diversity based on physicochemical properties (PCP) was defined as the Euclidean distance of six auto-scaled properties (SlogP, TPSA, AMW, RB, HBD, and HBA) and is shown as the filling of the data points using a continuous color scale. The relative number of compounds is represented with a different size of the data points (smaller data sets are represented with smaller data points).

Dataset 1.Schneidermatch.sdf. This file contains the list of the 78 matching scaffolds in SDF format, along with the original statistics calculated by Schneider et al..
No special software is required to open the SDF files. Any commercial or free software capable of reading SDF files will open the data sets supplied
Dataset 2.FooDBpolyphenols.sdf. This file contains 3,228 polyphenolic compounds available in FooDB, in SDF format.
No special software is required to open the SDF files. Any commercial or free software capable of reading SDF files will open the data sets supplied

Conclusions

FooDB is a novel, large and diverse library containing information of more than 23,000 compounds found in food. To date, it is the most informative public resource of food compounds. Visual representation of the chemical space revealed that FooDB largely contains and upgrades structural information from GRAS. Indeed, most of GRAS is contained in FooDB. Compounds in FooDB have a large diversity of physicochemical properties. The distributions of most physicochemical properties of FooDB compounds overlap with those of approved drugs and natural products in ZINC. GRAS mostly contains small-sized compounds. The global diversity indicates that FooDB has a large structural diversity as measured by molecular fingerprints, though it has relatively low scaffold diversity. One third of the compounds in FooDB are acyclic. The most frequent cyclic scaffolds are monocyclic. Of note, polyphenols represent a large fraction of FooDB. The list of 3,228 polyphenolic compounds identified in this work to enhance the on-going polyphenol-protein interactome studies. Analysis of the chemical complexity revealed that compounds in FooDB are more complex than approved drugs and natural products and have complexity comparable to GRAS compounds. A next step of this work is to compare the chemical space of FooDB with that of natural products from different sources, e.g., plants, terrestrial, cyanobacteria. A second suggested future study is to perform the virtual screening of FooDB across a range of targets, for instance, the increasingly important epigenetic targets (Naveja & Medina-Franco, 2018). Virtual screening can be done using multiple methods, for instance, using similarity searching. In this case one needs to consider, however, the potential presence of activity cliffs i.e., compounds with similar structure but different activity (Stumpfe et al., 2014). The goal of such study would be to identify systematically dietary components that may be participating in epigenetic regulatory processes (Martinez-Mayorga et al., 2013). These efforts are ongoing in our group and will be reported in due course. Other perspective of this work is integrating the knowledge of FooDB with other large databases with the aim of identifying food-disease associations and food-drug interactions such as the works previously published by Jensen et al. (Jensen et al., 2014; Jensen et al., 2015).

Data availability

Dataset 1: (Schneidermatch.sdf). This file contains the list of the 78 matching scaffolds in SDF format, along with the original statistics calculated by Schneider et al. No special software is required to open the SDF files. Any commercial or free software capable of reading SDF files will open the data sets supplied. 10.5256/f1000research.15440.d209071 (Naveja, et al., 2018a)

Dataset 2: (FooDBpolyphenols.sdf). This file contains 3,228 polyphenolic compounds available in FooDB, in SDF format. No special software is required to open the SDF files. Any commercial or free software capable of reading SDF files will open the data sets supplied. 10.5256/f1000research.15440.d209072 (Naveja et al., 2018b)

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Naveja JJ, Rico-Hidalgo MP and Medina-Franco JL. Analysis of a large food chemical database: chemical space, diversity, and complexity [version 2; peer review: 3 approved]. F1000Research 2018, 7(Chem Inf Sci):993 (https://doi.org/10.12688/f1000research.15440.2)
NOTE: If applicable, it is important to ensure the information in square brackets after the title is included in all citations of this article.
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ApprovedThe paper is scientifically sound in its current form and only minor, if any, improvements are suggested
Approved with reservations A number of small changes, sometimes more significant revisions are required to address specific details and improve the papers academic merit.
Not approvedFundamental flaws in the paper seriously undermine the findings and conclusions
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Reviewer Report 14 Aug 2018
Piotr Minkiewicz, Department of Food Biochemistry, Faculty of Food Science, University of Warmia and Mazury in Olsztyn, Olsztyn-Kortowo, Poland 
Approved
VIEWS 19
I fully approve the recent version of the artcle. I can recommend it ... Continue reading
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Minkiewicz P. Reviewer Report For: Analysis of a large food chemical database: chemical space, diversity, and complexity [version 2; peer review: 3 approved]. F1000Research 2018, 7(Chem Inf Sci):993 (https://doi.org/10.5256/f1000research.17367.r37062)
NOTE: it is important to ensure the information in square brackets after the title is included in all citations of this article.
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PUBLISHED 03 Jul 2018
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Reviewer Report 07 Aug 2018
Rachelle J. Bienstock, RJB Computational Modeling LLC, Chapel Hill, NC, USA 
Approved
VIEWS 26
The paper on chemical diversity of FooDB compared to several other databases, including GRAS and DrugBank and drug-like natural products fromZINC12, by Naveja, Rico-Hidalgo, and Medina-Franco was an interesting, informative and nicely presented analysis.  The figures and graphical presentation of ... Continue reading
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Bienstock R. Reviewer Report For: Analysis of a large food chemical database: chemical space, diversity, and complexity [version 2; peer review: 3 approved]. F1000Research 2018, 7(Chem Inf Sci):993 (https://doi.org/10.5256/f1000research.16825.r36226)
NOTE: it is important to ensure the information in square brackets after the title is included in all citations of this article.
  • Author Response 10 Aug 2018
    José L. Medina-Franco, Department of Pharmacy, School of Chemistry, Universidad Nacional Autónoma de México, Mexico City, 04510, Mexico
    10 Aug 2018
    Author Response
    We are grateful for the positive comments and thank the reviewer for the excellent suggestions to expand this work in future studies.
    Competing Interests: I have no competing interests.
COMMENTS ON THIS REPORT
  • Author Response 10 Aug 2018
    José L. Medina-Franco, Department of Pharmacy, School of Chemistry, Universidad Nacional Autónoma de México, Mexico City, 04510, Mexico
    10 Aug 2018
    Author Response
    We are grateful for the positive comments and thank the reviewer for the excellent suggestions to expand this work in future studies.
    Competing Interests: I have no competing interests.
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Reviewer Report 30 Jul 2018
Khushbu Shah, Division of Medicinal Chemistry, Graduate School of Pharmaceutical Sciences, Duquesne University, Pittsburgh, PA, USA;  Kramer Levin Naftalis Frankel LLP, New York, NY, USA 
Approved
VIEWS 21
This manuscript purports to analyze and disclose the chemical diversity of the FooDB database. It is an interesting study with a logical flow based on appropriate methods.

There a few optional suggestions that the authors could adapt ... Continue reading
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Shah K. Reviewer Report For: Analysis of a large food chemical database: chemical space, diversity, and complexity [version 2; peer review: 3 approved]. F1000Research 2018, 7(Chem Inf Sci):993 (https://doi.org/10.5256/f1000research.16825.r36288)
NOTE: it is important to ensure the information in square brackets after the title is included in all citations of this article.
  • Author Response 10 Aug 2018
    José L. Medina-Franco, Department of Pharmacy, School of Chemistry, Universidad Nacional Autónoma de México, Mexico City, 04510, Mexico
    10 Aug 2018
    Author Response
    We really appreciate the reviewer´s feedback and value the optional suggestions. In the revised manuscript we added the rationale for selecting the ´specific version of the three data sets. We ... Continue reading
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  • Author Response 10 Aug 2018
    José L. Medina-Franco, Department of Pharmacy, School of Chemistry, Universidad Nacional Autónoma de México, Mexico City, 04510, Mexico
    10 Aug 2018
    Author Response
    We really appreciate the reviewer´s feedback and value the optional suggestions. In the revised manuscript we added the rationale for selecting the ´specific version of the three data sets. We ... Continue reading
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Reviewer Report 11 Jul 2018
Piotr Minkiewicz, Department of Food Biochemistry, Faculty of Food Science, University of Warmia and Mazury in Olsztyn, Olsztyn-Kortowo, Poland 
Approved with Reservations
VIEWS 31
I have no critical remarks concerning methods, correctness of work. Discussion is also appropriate from the point of view of scientists working in the areas of cheminformatics and/or pharmacology.
I would like to ask some questions concerning relevance of ... Continue reading
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Minkiewicz P. Reviewer Report For: Analysis of a large food chemical database: chemical space, diversity, and complexity [version 2; peer review: 3 approved]. F1000Research 2018, 7(Chem Inf Sci):993 (https://doi.org/10.5256/f1000research.16825.r35684)
NOTE: it is important to ensure the information in square brackets after the title is included in all citations of this article.
  • Author Response 16 Jul 2018
    José L. Medina-Franco, Department of Pharmacy, School of Chemistry, Universidad Nacional Autónoma de México, Mexico City, 04510, Mexico
    16 Jul 2018
    Author Response
    Thank the reviewer for critically reading our manuscript and the valuable feedback. Hereunder we provide a point-by-point response to each comment.
     
    Comment: "I have no critical remarks concerning methods, correctness ... Continue reading
COMMENTS ON THIS REPORT
  • Author Response 16 Jul 2018
    José L. Medina-Franco, Department of Pharmacy, School of Chemistry, Universidad Nacional Autónoma de México, Mexico City, 04510, Mexico
    16 Jul 2018
    Author Response
    Thank the reviewer for critically reading our manuscript and the valuable feedback. Hereunder we provide a point-by-point response to each comment.
     
    Comment: "I have no critical remarks concerning methods, correctness ... Continue reading

Comments on this article Comments (0)

Version 2
VERSION 2 PUBLISHED 03 Jul 2018
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Alongside their report, reviewers assign a status to the article:
Approved - the paper is scientifically sound in its current form and only minor, if any, improvements are suggested
Approved with reservations - A number of small changes, sometimes more significant revisions are required to address specific details and improve the papers academic merit.
Not approved - fundamental flaws in the paper seriously undermine the findings and conclusions
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