The use of electron microscopy in the diagnosis of focal segmental glomerulosclerosis: are current pathological techniques missing important abnormalities in the glomerular basement membrane?

Justin Davis; Alwie Tjipto; Katharine Hegerty; Andrew Mallett

doi:10.12688/f1000research.19997.2

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Research Article

Revised

The use of electron microscopy in the diagnosis of focal segmental glomerulosclerosis: are current pathological techniques missing important abnormalities in the glomerular basement membrane?

[version 2; peer review: 2 approved]

Justin Davis ¹, Alwie Tjipto¹, Katharine Hegerty², Andrew Mallett²

PUBLISHED 07 Nov 2019

Author details Author details

¹ Department of Nephrology, Barwon Health, Geelong, Victoria, 3220, Australia
² Department of Nephrology, Royal Brisbane and Women's Hospital, Herston, Queensland, Australia

Justin Davis
Roles: Conceptualization, Data Curation, Formal Analysis, Investigation, Methodology, Project Administration, Resources, Software, Validation, Visualization, Writing – Original Draft Preparation, Writing – Review & Editing

Alwie Tjipto
Roles: Conceptualization, Data Curation, Formal Analysis, Investigation, Methodology, Project Administration, Resources, Software, Supervision, Validation, Visualization, Writing – Original Draft Preparation, Writing – Review & Editing

Katharine Hegerty
Roles: Conceptualization, Data Curation, Formal Analysis, Investigation, Methodology, Project Administration, Resources, Software, Validation, Visualization, Writing – Original Draft Preparation, Writing – Review & Editing

Andrew Mallett
Roles: Conceptualization, Data Curation, Formal Analysis, Investigation, Methodology, Project Administration, Resources, Software, Supervision, Validation, Visualization, Writing – Original Draft Preparation, Writing – Review & Editing

OPEN PEER REVIEW

REVIEWER STATUS

Abstract

Background: There is an increasing appreciation that variants of the COL4A genes may be associated with the development of focal segmental glomerulosclerosis (FSGS). On electron microscopy, such variants may produce characteristic changes within the glomerular basement membrane (GBM). These changes may be missed if glomerular lesions histologically diagnosed as FSGS on light microscopy are not subjected to electron microscopy.
Methods: We conducted a retrospective cohort analysis of all patients presenting to two hospitals who received a primary histological diagnosis of FSGS to see if these samples underwent subsequent electron microscopy. Each such sample was also scrutinised for the presence of characteristic changes of an underlying type IV collagen disorder
Results: A total of 43 patients were identified. Of these, only 30 underwent electron microscopy. In two samples there were histological changes detected that might have suggested the underlying presence of a type IV collagen disorder. Around one in three biopsy samples that had a histological diagnosis of FSGS were not subjected to electron microscopy.
Conclusion: Renal biopsy samples that have a histological diagnosis of primary FSGS not subjected to subsequent electron microscopy may potentially miss ultrastructural changes in the GBM that could signify an underlying type IV collagen disorder as the patient’s underlying disease process. This could potentially affect both them and their families’ investigative and management decisions given potential for implications for transplant, heritability and different disease pathogenesis. This represents a gap in care which should be reflected upon and rectified via iterative standard care and unit-level quality assurance initiatives.

Keywords

Collagen IV, Electron Microscopy, Glomerular Basement Membrane, Focal Segmental Glomerulosclerosis, Renal Biopsy.

Corresponding author: Justin Davis

Competing interests: No competing interests were disclosed.

Grant information: The author(s) declared that no grants were involved in supporting this work.

Copyright: © 2019 Davis J et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

How to cite: Davis J, Tjipto A, Hegerty K and Mallett A. The use of electron microscopy in the diagnosis of focal segmental glomerulosclerosis: are current pathological techniques missing important abnormalities in the glomerular basement membrane? [version 2; peer review: 2 approved]. F1000Research 2019, 8:1204 (https://doi.org/10.12688/f1000research.19997.2) First published: 26 Jul 2019, 8:1204 (https://doi.org/10.12688/f1000research.19997.1) Latest published: 07 Nov 2019, 8:1204 (https://doi.org/10.12688/f1000research.19997.2)

Revised Amendments from Version 1

To F1000 research as well as the two reviewers who kindly reviewed our article;

On behalf of my co-authors, I wish to thank not only F1000 research but also the two reviewers who took the time out of their busy work schedule to read through our manuscript and make suggestions which only serve to strengthen the article overall and make it a better piece for submission.

Major changes include:

1. The authors agree that the extrapolation of EM changes in COL4A variants could be further elaborated on, and have introduced a new section in the introduction looking at a Chinese cohort with both confirmed EM and COL4A mutations. Unfortunately due to small sample sizes, the sensitivity of such findings cannot be commented on at this time.

2. The authors have included a new figure (figure 2) with a proposed worksheet for investigation.

3. Unfortunately, the patients whom had EM abnormalities suggestive of a type IV collagen disorder have been lost to follow up, and the data on them is simply not there (a shame as the authors were curious about such things too)

Minor changes include:

1. The title has been reworded to avoid confusion, and text within the paragraphs altered

2. The Y axis is now labelled appropriately.

3. Tip and NOS, now specified within the manuscripts main paragraphs.

4. The authors thank the reviewers for pointing out small errors of inconsistency which can creep into a document like this, and have amended them appropriately.

See the authors' detailed response to the review by Constantinos Deltas
See the authors' detailed response to the review by James McCaffrey and Rachel Lennon

Abbreviations

Chronic kidney disease (CKD); end stage kidney disease (ESKD); focal segmental glomerulosclerosis (FSGS); glomerular basement membrane (GBM); not otherwise specified (NOS); thin basement membrane nephropathy (TBMN).

Introduction

Focal segmental glomerulosclerosis (FSGS) is a pathological diagnosis which underpins a variety of progressive renal diseases that commonly result in proteinuria, chronic kidney disease (CKD) and potential end stage kidney disease (ESKD) requiring renal replacement therapy¹. FSGS occurs in either a primary. secondary, or genetic form. Primary FSGS is thought to be largely immunological in nature perhaps driven by an elusive permeability factor and secondary FSGS caused by compensatory hyperfiltration due to a previous glomerular injury². In recent years there has been a greater appreciation of the contribution of underlying genetic causes of this histological pattern. Many of these genetic aetiologies for FSGS have potential clinical significance for at-risk family members, subsequent genetic counselling, future living related kidney transplantation and therapeutics including potential avoidance of immunosuppressive therapies otherwise used for primary immunologically-mediated FSGS. Although initial focus has been on genes that are involved in the maintenance of podocyte structure and function it has become apparent that abnormalities in genes responsible for the structural integrity of the glomerular basement membrane (GBM) may underpin a significant minority of cases of adult-onset FSGS³. Variants in COL4A3, COL4A4 and COL4A5 which encode the α3, α4 and α5 chains of type IV collagen respectively, the major constituent of the GBM previously linked to both Alport syndrome and thin basement membrane nephropathy (TBMN) may also underlie cases of FSGS⁴. This suggestion has biological plausibility. Podocytes, the glomerular epithelial cells responsible for maintenance of the filtration barrier of the glomerulus through which plasma is ultrafiltrated, are not only a source for the α-chains secreted to form the GBM but also are required to be anchored to this structure in order to maintain the aforementioned filtration barrier^2,5. It is not inconceivable then that inherited structural abnormalities of the GBM may lead to subsequent podocyte dysfunction and ultimately the lesion histologically characterised as FSGS.

The relationship between the three renal conditions intertwined around variants in the COL4A genes, Alport syndrome, TBMN, and FSGS is complex and incompletely understood. Whilst previously it was believed that patients heterozygous for variants in COL4A3 and COL4A4 would develop TBMN with persistent microscopic haematuria and an otherwise benign prognosis, this traditional thinking has been overturned by the discovery that some pedigrees with these variants will go on to develop significant proteinuria, FSGS lesions and the potential for progressive CKD or ESKD^6,7. More recently, targeted gene sequencing of adults thought to have primary FSGS or steroid resistant nephrotic syndrome (the paediatric equivalent) found that pathogenic or likely pathogenic variants in COL4A genes were present in up to 38% of families with familial FSGS, and 3% of those with sporadic FSGS^3,8. These findings highlight the importance of considering variants in these genes in the diagnosis and subsequent management of patients found to have FSGS histologically.

FSGS is a clinicopathological diagnosis that is made on the review of renal tissue obtained by percutaneous biopsy. This tissue is processed and subjected to several different staining techniques and methods of microscopy, including light, immunofluorescence and electron microscopy⁹. FSGS, suggested on light microscopy by the finding of focal, segmental lesions with sclerosis, and an increase in the glomerular matrix with obliteration of the capillary lumens only requires this finding in one glomerulus and light microscopy to diagnose². As such, some specimens may previously have not been sent for further processing and review with other techniques such as electron microscopy even though this histopathology has heterogenous causative aetiologies. The other potential renal lesions that may be caused by COL4A gene variants, Alport syndrome and TBMN, cause thinning, lamellation and fraying of the GBM and may also be associated with podocyte foot process effacement, all of which require electron microscopy to visualise⁴. Similar findings may also be present in FSGS cases associated with COL4A variants. One study of Chinese families with familial FSGS noted segmental thinning of the GBM similar to those which may be present with TBMN in four out of five families with a proven COL4A variant underlying their familial FSGS (electron microscopy in another family and a sporadic case with proven variants were both noted to be unremarkable)¹⁰. At present the sensitivity of these findings remain unknown. It should be noted that for Alport syndrome there are other techniques aside from electron microscopy available for identifying the diagnosis. Immunostaining of a renal biopsy specimen for type IV collagen may demonstrate the absence of alpha 3,4 or 5 chains in up to two thirds of patients, or a skin biopsy may show an absence of an alpha 5 chain^11,12, in addition to the aforementioned genetic testing. Such absence by immunostaining does not appear be present in FSGS¹³. Given that the pathological diagnosis of FSGS does not routinely require electron microscopy it is therefore conceivable that GBM lesions potentially associated with an underlying type IV collagen variant may have been missed. This represents an opportunity to reflect on our prior clinical behaviour to see if our multidisciplinary diagnostic practice may require improvement. We conducted a retrospective cohort analysis of prior renal biopsy results in two tertiary centres to see how many of those that had been given a histological diagnosis of FSGS were sent for subsequent electron microscopy.

Methods

Study background

Our study was a retrospective cohort analysis across two tertiary hospital sites involving the review of prior renal biopsy results that had been given the histological diagnosis of FSGS on light microscopy and how many of these samples subsequently went on to be processed for electron microscopy. In addition, of those samples that were subjected to electron microscopy, we reviewed how many displayed evidence of a potential type IV collagen disorder. This project had ethics approval through Barwon Health’s Research Ethics Governance and Integrity unit and the Royal Brisbane and Women’s Hospital Human Research and Ethics Committee (HREC 18/131 and HREC/18/QRBW/258, respectively). Participants consent was waived by both ethics committees due to the negligible risk nature of data acquisition via retrospective datasets already maintained on electronic health records at both institutions.

Eligibility criteria

Patients aged over the age of 18 who received a histological diagnosis of FSGS on native kidney biopsy during the time period of January 1^st 2008 until July 31^st 2018 at the first participating hospital and from the 1^st of October 2013 until the 29^th of December 2018 at the second were eligible to be included in the retrospective audit. Participants were excluded from the audit if they were less than 18 years of age, underwent kidney transplant biopsy, were defined clinically to have secondary FSGS as opposed to primary disease, received their diagnosis of FSGS or underwent a renal biopsy and pathological review outside of the prespecified time period for each institution.

Sample treatment

Samples for biopsy were placed in the following fixatives for processing; formalin for light microscopy, saline soaked gauze with freezing for immunofluorescence, and glutaraldehyde for electron microscopy. Samples for light microscopy are embedded and sectioned at 2- to 3-µm thickness with hematoxylin-eosin, Jones silver, periodic acid-schiff and trichrome staining performed. Immunofluorescence samples are sectioned within a cryostat and placed on prelabelled air-dried slides of the antigen in question. Electron microscopy tissue is processed into plastic, trimmed, cut into a 1-µm section and stained with toludine blue. The images are reviewed in a digital medium¹⁴.

Identification of participants and data analysis

A search strategy was developed for this retrospective cohort study to identify appropriate patients through local electronic medical record systems using the search term ‘focal segmental glomerulosclerosis’ with the AND operator to combine with ‘glomerulonephritis’ or ‘hereditary nephritis’. These patient results were subsequently reviewed for evidence of a previous percutaneous renal biopsy and report. The data were independently extracted from the included patients by the primary reviewer and collated in a Microsoft Excel document that included information on age, primary diagnosis, included use of electron microscopy and any changes that may be consistent with an underlying collagen disorder including thinning, lamellation and fraying of the GBM. The data extracted was verified by the other three co-authors with all discrepancies resolved through discussion and consensus.

Results

Participant information and diagnoses

From January 2008 through to July 2018 at the first centre and October 2013 until December 2018 at the second, a total of 43 patients were identified as having primary FSGS. The baseline characteristics of the study cohort are provided in Table 1. The median age was 49 years and the patients were predominantly male (55%). The most common underlying histological diagnosis reported in the renal biopsy clinical pathology reports was FSGS not otherwise specified (NOS), followed by familial FSGS with no underlying genetic disorder ascertained. There were small numbers of the cellular, collapsing, perihilar and tip FSGS variants noted. The two most common stages of CKD at the time of presentation were I and III. The results extracted and analyses in this study are available as Underlying data¹⁵.

Table 1. Demographic and clinical characteristics of the patients at baseline.

CHARACTERISTIC	Value, n (%) (unless indicated)
Age, years*	Median (range) 49.95 (23–88)
Sex
Male	24 (55.81)
Female	19 (44.18)
Underlying diagnosis
FSGS NOS	31 (72.09)
FSGS cellular variant	2 (4.65)
FSGS collapsing variant	1 (2.32)
FSGS Familial	3 (6.97)
FSGS Perihilar	3 (6.97)
FSGS Tip	3 (6.97)
Electron microscopy report available for biopsy?
Yes	30 (69.76)
No	13 (30.23)
Collagen disorder suggested	2 (2.81)
Stage at diagnosis
I	12
II	9
III	12
IV	8
V	2
Unknown	0

*Values given as median (range).

Use of electron microscopy

Of the 43 patients identified, samples from 30 underwent electron microscopy after initial light microscopy and immunofluorescence. Two of these samples showed signs on electron microscopy that might be consistent with an underlying type IV collagen glomerular basement membrane disorder. Microscopic haematuria was not noted in either of these two patients. Of the 13 samples that did not undergo electron microscopy, four had no glomeruli present within the processed core. The remaining nine samples had no documented reason.

The overall percentage of biopsy samples for which data were available that were not subjected to electron microscopy was 30%, of which close to 21% had no documented reason for not undergoing electron microscopy. The annualised rate of biopsy samples that were not subjected to electron microscopy varied with time (Figure 1). The number of biopsy reports available for analysis prior to 2013 was only one per year at most; however, from 2013 onwards the rate progressively increased to between 3 and 11 cases of FSGS diagnosed at histopathology between the two institutions per year. The annualised rate of electron microscopy from 2013 onwards varied each year, ranging from 50 to 87%. Overall 30% of biopsy samples did not receive electron microscopy. The overall rate of electron microscopy did not significantly change across the study period between the two centres.

Figure 1. Annualised rate of biopsy samples subjected to electron microscopy.

Discussion

This retrospective cohort analysis demonstrated that about two-thirds of native kidney biopsy samples across two institutions that were deemed to have primary FSGS underwent subsequent electron microscopy. Of those that did, two were reported to have characteristics that might be consistent with an underlying type IV collagen disorder. In both samples, electron microscopy revealed a diffusely thin GBM, with the second additionally identifying early focal splitting of the GBM. The first sample, in which the patient had the tip variant of FSGS, was suggested to be consistent with TBMN whereas there was no pathological comment made about the second from a patient with FSGS NOS. FSGS (NOS) was the most common lesion described in this study, consistent with prior reports². Notably, close to one in three cases of primary FSGS were not proceeding to electron microscopy despite an indication to do so and 1 in 20 cases within our cohort had structural changes that were consistent with an underlying type IV collagen variant. Whilst some samples were unable to undergo electron microscopy due to a lack of glomeruli in the biopsy core, in the majority of the others it is unclear why subsequent electron microscopy did not occur. The annualised rate of biopsy samples not subjected to electron microscopy varied by year, but on average around one in three samples were not subjected to electron microscopy despite receiving a histological diagnosis of FSGS.

Curiously, neither of the two patients in whom electron microscopy was suggestive of an underlying type IV collagen disorder was noted to have haematuria on their urinalysis at the time of presentation. Both of these samples were noted to have a thin basement membrane on electron microscopy, with focal splitting noted in one with an average thickness of 230.72 nm given. This is in keeping with a study of Chinese FSGS families in which four patients with a demonstrated mutation in COL4A and familial FSGS all had findings suggestive of TBMN on electron microscopy¹⁰. Unfortunately due to the retrospective nature of this study we were unable to send any samples for immunostaining of type IV collagen. Regrettably he authors also do not have any data as to whether the two patients in whom electron microscopy was suggestive of a type IV collagen disorder proceeded to subsequent genetic testing for COL4A mutations or whether they were subjected to immunosuppression and their subsequent clinical course. The authors suggest that sequencing of these electron microscopy variants when found could be an extension of current research to expand our knowledge in this field.

There is an increasing body of evidence indicating that inheritable variants in COL4A may underlie a proportion of cases of FSGS, with up to 12.5% cases of autosomal dominant FSGS attributable to COL4A3 in some cohorts¹⁰. Not subjecting these renal biopsy samples to electron microscopy represents a potential gap in the investigation and subsequent management of such patients given they are much less likely to respond to immunosuppressive therapy¹⁶ which has otherwise been classically indicated (Figure 2).

Figure 2. Proposed workflow for investigation of patients in this demographic.

There is an increasing appreciation that some adult patients with otherwise apparently sporadic FSGS with a primary non-response to immunosuppressive therapy will harbour a genetic form of FSGS, which should be investigated appropriately. We propose that any patient with electron microscopy features suggestive of a type IV collagen variant proceed to genetic testing for confirmation. Familial cases should proceed to sequencing^8,17,18.

This study was designed as a retrospective cohort study looking at the number of samples sent for electron microscopy, as well as any potential changes which might be consistent with a type IV collagen glomerular basement membrane disorder. It is important to recognise that not all groups have found the characteristic changes associated with the type IV collagen disorders such as Alport’s Syndrome or TBMN on electron microscopy. One study described the typical pathological changes of FSGS but not the glomerular basement membrane abnormalities characterising Alport syndrome or TBMN in patients known to have variants in either COL4A3 or COL4A4⁷. It is thus possible that a lack of classical findings for a type IV collagen glomerular basement membrane disorder may have accounted for the low number of those with GBM features on electron microscopy consistent with a type IV collagen disorder noted within our study. Indicating against this, however, another study suggested that biopsy samples from patients with the classical features of Alport Syndrome or TBMN showed podocyte detachment which might be expected and subsequently cause FSGS-type lesions⁴. Other studies which have looked at electron microscopy in FSGS cases have similarly found low numbers of abnormalities that may be consistent with an underlying collagen 4 disorder^3,8, which suggests the overall number of abnormalities to be found via electron microscopy may be low.

The process by which variants within the COL4A genes might cause FSGS remains unclear, particularly given their clear association with Alport Syndrome and TBMN. One proposal is that the ultrastructural changes induced by the type IV collagen variants, perhaps under the influence of modifier genes such as laminin, result in impaired podocyte attachment to the glomerular basement membrane which leads to accelerated podocyte detachment, subsequent foot process effacement as a response to the increased shear stress induced by the denuded basement membrane and at a critical level of podocyte loss collapse of the capillary network with the appearance of the classical segmental sclerotic lesion^2,4,19. It also remains unclear as to whether the changes of FSGS are a secondary process occurring in those with TBMN or whether the type IV collagen variants are capable of causing primary FSGS^7,20. FSGS occurring as a secondary process to other basement membrane abnormalities may explain why immunosuppressive therapy has traditionally been less effective in inherited forms of FSGS, although there are case reports of the successful use of the calcineurin inhibitor cyclosporine for some patients harbouring inheritable type IV collagen disorders⁶.

In summary, this study has found that not all biopsy samples that had primary FSGS as a histological diagnosis were subjected to subsequent electron microscopy. This may have potentially led to inadvertently overlooking characteristic basement membrane abnormalities, which may suggest an underlying and heritable type IV collagen disorder. These findings reflect an opportunity to change practice in order to better investigate, counsel and provide clinical management to these and future patients.

Data availability

Underlying data

Figshare: Davis et al. FSGS biopsy audit.xlsx. https://doi.org/10.6084/m9.figshare.8949032.v1¹⁵.

This project contains the variables extracted for each individual retrospectively assessed in this study.

Data are available under the terms of the Creative Commons Attribution 4.0 International license (CC-BY 4.0).

Author contributions

All authors made substantial intellectual contributions to the manuscript. The audit was overseen by the lead authors. All authors participated in the interpretation of data and vouch for the completeness and accuracy of the data. The first author (JD) wrote the first draft with all authors involved in the conception and design of the manuscript. All authors participated in the development of this manuscript and made the decision to publish the results. The acquisition, analysis and interpretation of data was led by the corresponding author JD with equally important contributions and oversight including the resourcing of key references from AT, KH and AM. All authors were both involved in the drafting of the manuscript and critical revision for important intellectual content. All authors have given final approval for the version to be published. Each author has participated sufficiently in the work to take public responsibility for appropriate portions of the content and both authors agree to be accountable for all aspects of the work in ensuring that questions related to the accuracy or integrity of the work are appropriately investigated and resolved.

Faculty Opinions recommended

References

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2. De Vriese AS, Sethi S, Nath KA, et al.: Differentiating Primary, Genetic, and Secondary FSGS in Adults: A Clinicopathologic Approach. J Am Soc Nephrol. 2018; 29(3): 759–74. PubMed Abstract | Publisher Full Text | Free Full Text
3. Gast C, Pengelly RJ, Lyon M, et al.: Collagen (COL4A) mutations are the most frequent mutations underlying adult focal segmental glomerulosclerosis. Nephrol Dial Transplant. 2016; 31(6): 961–70. PubMed Abstract | Publisher Full Text
4. Wickman L, Hodgin JB, Wang SQ, et al.: Podocyte Depletion in Thin GBM and Alport Syndrome. PLoS One. 2016; 11(5): e0155255. PubMed Abstract | Publisher Full Text | Free Full Text
5. Korstanje R, Caputo CR, Doty RA, et al.: A mouse Col4a4 mutation causing Alport glomerulosclerosis with abnormal collagen α3α4α5(IV) trimers. Kidney Int. 2014; 85(6): 1461–8. PubMed Abstract | Publisher Full Text | Free Full Text
6. Pierides A, Voskarides K, Athanasiou Y, et al.: Clinico-pathological correlations in 127 patients in 11 large pedigrees, segregating one of three heterozygous mutations in the COL4A3/ COL4A4 genes associated with familial haematuria and significant late progression to proteinuria and chronic kidney disease from focal segmental glomerulosclerosis. Nephrol Dial Transplant. 2009; 24(9): 2721–9. PubMed Abstract | Publisher Full Text
7. Malone AF, Phelan PJ, Hall G, et al.: Rare hereditary COL4A3/COL4A4 variants may be mistaken for familial focal segmental glomerulosclerosis. Kidney Int. 2014; 86(6): 1253–9. PubMed Abstract | Publisher Full Text | Free Full Text
8. Yao T, Udwan K, John R, et al.: Integration of Genetic Testing and Pathology for the Diagnosis of Adults with FSGS. Clin J Am Soc Nephrol. 2019; 14(2): 213–223, pii: CJN.08750718. PubMed Abstract | Publisher Full Text | Free Full Text
9. Hogan JJ, Mocanu M, Berns JS: The Native Kidney Biopsy: Update and Evidence for Best Practice. Clin J Am Soc Nephrol. 2016; 11(2): 354–62. PubMed Abstract | Publisher Full Text | Free Full Text
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11. Kashtan CE: Alport syndrome. An inherited disorder of renal, ocular, and cochlear basement membranes. Medicine (Baltimore). 1999; 78(5): 338–60. PubMed Abstract | Publisher Full Text
12. Massella L, Onetti Muda A, Faraggiana T, et al.: Epidermal basement membrane alpha 5(IV) expression in females with Alport syndrome and severity of renal disease. Kidney Int. 2003; 64(5): 1787–91. PubMed Abstract | Publisher Full Text
13. Cai YI, Sich M, Beziau A, et al.: Collagen distribution in focal and segmental glomerulosclerosis: an immunofluorescence and ultrastructural immunogold study. J Pathol. 1996; 179(2): 188–96. PubMed Abstract | Publisher Full Text
14. Walker PD: The renal biopsy. Arch Pathol Lab Med. 2009; 133(2): 181–8. PubMed Abstract
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16. Papazachariou L, Papagregoriou G, Hadjipanagi D, et al.: Frequent COL4 mutations in familial microhematuria accompanied by later-onset Alport nephropathy due to focal segmental glomerulosclerosis. Clin Genet. 2017; 92(5): 517–27. PubMed Abstract | Publisher Full Text
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Version 2

VERSION 2 PUBLISHED 26 Jul 2019

Author details Author details

¹ Department of Nephrology, Barwon Health, Geelong, Victoria, 3220, Australia
² Department of Nephrology, Royal Brisbane and Women's Hospital, Herston, Queensland, Australia

Competing interests

No competing interests were disclosed.

Grant information

The author(s) declared that no grants were involved in supporting this work.

Article Versions (2)

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https://doi.org/10.12688/f1000research.19997.2

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https://doi.org/10.12688/f1000research.19997.1

© 2019 Davis J et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Davis J, Tjipto A, Hegerty K and Mallett A. The use of electron microscopy in the diagnosis of focal segmental glomerulosclerosis: are current pathological techniques missing important abnormalities in the glomerular basement membrane? [version 2; peer review: 2 approved]. F1000Research 2019, 8:1204 (https://doi.org/10.12688/f1000research.19997.2)

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Open Peer Review

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Reviewer Report 13 Nov 2019

Rachel Lennon, Wellcome Trust Centre for Cell-Matrix Research, Faculty of Biology, Medicine and Health, University of Manchester, Manchester, UK

James McCaffrey, Department of Histopathology, Cambridge University Hospitals NHS Foundation Trust, Cambridge, UK

Approved

https://doi.org/10.5256/f1000research.23359.r56307

In this revised version of the manuscript we are satisfied that the authors have made improvements where these were possible. In particular data regarding the specificity of GBM changes found by EM for identifying familial FSGS (4/5 families with a proven COL4A variant and FSGS had GBM thinning). We accept that sensitivity of EM abnormalities is difficult to ascertain.

It is unfortunate that the patients with GBM abnormalities identified in the present study have been lost to follow up. However, we thank the authors for making efforts to retrieve clinical information, which may have strengthened the argument that the patients did harbour a COL4A variant.

The multi-modality approach to identifying patients with an underlying genetic cause for FSGS is more clearly emphasised. We agree with the workflow shown in Figure 2 that if GBM structural abnormalities are identified by EM in cases of sporadic FSGS, patients should be thoroughly investigated for a underlying genetic cause by NGS. This may also be relevant in familial cases where GBM abnormalities are identified by electron microscopy.

The title of the article now more accurately reflects the data provided.

We are happy to approve this article without further revisions.

Competing Interests: No competing interests were disclosed.

We confirm that we have read this submission and believe that we have an appropriate level of expertise to confirm that it is of an acceptable scientific standard.

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Reviewer Report 28 Oct 2019

Constantinos Deltas, Molecular Medicine Research Center, Department of Biological Sciences, University of Cyprus, Nicosia, Cyprus

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https://doi.org/10.5256/f1000research.21949.r54539

This retrospective study focuses on the fact that renal biopsies are not always evaluated by electron microscopy (EM). The authors found 43 renal biopsies in the records of two hospitals, where the diagnosis of FSGS was made, for which EM had been performed only in 30. Most importantly, in two of the biopsies studied by EM, there was evidence for a collagen IV disorder but had not been followed up. This has important implications in view of numerous reports in the past decade and more, according to which the histological diagnosis of FSGS could be on the background of collagen IV mutations, which cause Alport syndrome or thin basement membrane nephropathy.

I agree with all points raised by the previous reviewer and which I do not intend to repeat here. I would only emphasize two things:

I believe there should be no renal biopsy attempted if it is not going to be studied fully by light microscopy, immunofluorescence studies and EM. It is an invasive procedure accompanied by some risk and when performed it must be fully exploited as very significant results may come up, which bear implications on treatment, inheritance patterns and family planning.
The authors found two patients who had undergone EM and there were findings indicative of a collagen IV disorder. They should have proceeded to genetic testing to verify this and strengthen their case. Even belatedly, this could be of benefit not only to the patients but also to family members.

Is the work clearly and accurately presented and does it cite the current literature?

Yes
Is the study design appropriate and is the work technically sound?

Yes
Are sufficient details of methods and analysis provided to allow replication by others?

Yes
If applicable, is the statistical analysis and its interpretation appropriate?

Not applicable
Are all the source data underlying the results available to ensure full reproducibility?

No source data required
Are the conclusions drawn adequately supported by the results?

Yes

Competing Interests: No competing interests were disclosed.

Reviewer Expertise: Human molecular and medical genetics, nephrogenetics, cell biology and biochemistry

I confirm that I have read this submission and believe that I have an appropriate level of expertise to confirm that it is of an acceptable scientific standard.

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Author Response 07 Nov 2019

Justin Davis, Department of Nephrology, Barwon Health, Geelong, 3220, Australia

07 Nov 2019

Author Response

To the second reviewer who kindly reviewed our article;

On behalf of my co-authors, I wish to thank you for taking the time out of your busy work schedule to read ... Continue reading To the second reviewer who kindly reviewed our article;

On behalf of my co-authors, I wish to thank you for taking the time out of your busy work schedule to read through our manuscript and make suggestions which only serve to strengthen the article overall and make it a better piece for submission. We would like to ensure we have addressed both concerns pointed out below.

1. The authors agree with this point and feel most (if not all, at least in the case of native kidney) biopsies should be subjected to appropriate microscopic techniques which includes electron microscopy. It is unclear why some of our samples unfortunately were not, and remains a clear source of potential quality improvement that can be fed back, particularly when suggestive abnormalities may be found that influence further management.

2. The authors too are curious as to the genetic status of the two individuals who had suggestive EM changes noted within the body of the manuscript. Unfortunately on perusal of the data available they have been lost to follow up without a clear genetic diagnosis at this time.

Once again, many thanks for taking the time to review our work
To the second reviewer who kindly reviewed our article;

On behalf of my co-authors, I wish to thank you for taking the time out of your busy work schedule to read through our manuscript and make suggestions which only serve to strengthen the article overall and make it a better piece for submission. We would like to ensure we have addressed both concerns pointed out below.

1. The authors agree with this point and feel most (if not all, at least in the case of native kidney) biopsies should be subjected to appropriate microscopic techniques which includes electron microscopy. It is unclear why some of our samples unfortunately were not, and remains a clear source of potential quality improvement that can be fed back, particularly when suggestive abnormalities may be found that influence further management.

2. The authors too are curious as to the genetic status of the two individuals who had suggestive EM changes noted within the body of the manuscript. Unfortunately on perusal of the data available they have been lost to follow up without a clear genetic diagnosis at this time.

Once again, many thanks for taking the time to review our work
Competing Interests: None applicable. Close
Report a concern
Respond or Comment

COMMENTS ON THIS REPORT

Author Response 07 Nov 2019

Justin Davis, Department of Nephrology, Barwon Health, Geelong, 3220, Australia

07 Nov 2019

Author Response

To the second reviewer who kindly reviewed our article;

On behalf of my co-authors, I wish to thank you for taking the time out of your busy work schedule to read ... Continue reading To the second reviewer who kindly reviewed our article;

On behalf of my co-authors, I wish to thank you for taking the time out of your busy work schedule to read through our manuscript and make suggestions which only serve to strengthen the article overall and make it a better piece for submission. We would like to ensure we have addressed both concerns pointed out below.

1. The authors agree with this point and feel most (if not all, at least in the case of native kidney) biopsies should be subjected to appropriate microscopic techniques which includes electron microscopy. It is unclear why some of our samples unfortunately were not, and remains a clear source of potential quality improvement that can be fed back, particularly when suggestive abnormalities may be found that influence further management.

2. The authors too are curious as to the genetic status of the two individuals who had suggestive EM changes noted within the body of the manuscript. Unfortunately on perusal of the data available they have been lost to follow up without a clear genetic diagnosis at this time.

Once again, many thanks for taking the time to review our work
To the second reviewer who kindly reviewed our article;

On behalf of my co-authors, I wish to thank you for taking the time out of your busy work schedule to read through our manuscript and make suggestions which only serve to strengthen the article overall and make it a better piece for submission. We would like to ensure we have addressed both concerns pointed out below.

1. The authors agree with this point and feel most (if not all, at least in the case of native kidney) biopsies should be subjected to appropriate microscopic techniques which includes electron microscopy. It is unclear why some of our samples unfortunately were not, and remains a clear source of potential quality improvement that can be fed back, particularly when suggestive abnormalities may be found that influence further management.

2. The authors too are curious as to the genetic status of the two individuals who had suggestive EM changes noted within the body of the manuscript. Unfortunately on perusal of the data available they have been lost to follow up without a clear genetic diagnosis at this time.

Once again, many thanks for taking the time to review our work
Competing Interests: None applicable. Close
Report a concern

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Reviewer Report 18 Sep 2019

James McCaffrey, Department of Histopathology, Cambridge University Hospitals NHS Foundation Trust, Cambridge, UK

Rachel Lennon, Wellcome Trust Centre for Cell-Matrix Research, Faculty of Biology, Medicine and Health, University of Manchester, Manchester, UK

Approved with Reservations

https://doi.org/10.5256/f1000research.21949.r53353

This is a retrospective study of kidney biopsies from 43 patients over a 10-year interval. The authors retrieved native biopsies from patients with a primary histological diagnosis of FSGS in two tertiary hospitals. They determined the number of biopsies that had both histological evidence of FSGS and accompanying ultrastructural analysis with electron microscopy (EM). 13/43 biopsies did not have evaluation by EM. In 2 biopsies that had both histology and EM, abnormalities of the glomerular basement membrane (GBM) were identified. The authors advocate an increase in the use of EM with a diagnosis of FSGS to identify a subset of patients with an underlying genetic disorder affecting type IV collagen.

Overall the study is a simple, retrospective review design and it is well presented. It highlights the important observation and increasing recognition that pathogenic variants in COL4A genes are present in a high proportion of families with familial FSGS and also in individuals with sporadic FSGS. There is a pressing clinical need to identify these patients and, as such, this study comes at an opportune time. We have the following questions and suggestions for improvement:

The authors could present a stronger argument for EM being the investigation of choice for identifying patients with COL4A mutations. Although the authors discuss the variable EM findings in patients with Alport syndrome or TBMN, this could be expanded. How sensitive are the GBM findings for COL4 mutations? Not all patients with COL4A variants have typical EM findings, and furthermore suggestive GBM abnormalities may be found in other genetic disorders including mutations in MYH9 and LMX1B.
There would be additional value if the authors could propose a pros/cons comparison of EM versus genetic screening with for example an NGS gene panel to identify these patients. This could be presented as a proposed workflow for investigation.
The authors identify 2 patients with EM findings suggestive of a COL4A disorder. However, there are no data detailing whether these patients did in fact harbour a COL4A variant (notably neither of them had haematuria at presentation). Are any data available, for example, on clinical response to immunosuppression, or any sequencing data?

Minor comments

The title described the study as an 'audit' but the text repeatedly refers to a 'retrospective cohort analysis.' As there are no defined standards against which clinical practice is being measured here, perhaps remove 'audit' from the title?
The Y-axis in Figure 1 should be labelled.
Which FSGS variant did the 2 patients with EM abnormalities fall into?
Improve consistency with nomenclature—use of conventional gene (COL4A) or protein names (type IV collagen).

Is the work clearly and accurately presented and does it cite the current literature?

Yes
Is the study design appropriate and is the work technically sound?

Yes
Are sufficient details of methods and analysis provided to allow replication by others?

Yes
If applicable, is the statistical analysis and its interpretation appropriate?

Yes
Are all the source data underlying the results available to ensure full reproducibility?

No source data required
Are the conclusions drawn adequately supported by the results?

Yes

Competing Interests: No competing interests were disclosed.

Reviewer Expertise: Paediatric nephrology, matrix biology, glomerular cell biology, histopathology

We confirm that we have read this submission and believe that we have an appropriate level of expertise to confirm that it is of an acceptable scientific standard, however we have significant reservations, as outlined above.

CITE

Report a concern

Author Response 07 Nov 2019

Justin Davis, Department of Nephrology, Barwon Health, Geelong, 3220, Australia

07 Nov 2019

Author Response

To the first reviewer who kindly reviewed our article;

On behalf of my co-authors, I wish to thank you for taking the time out of your busy work schedule ... Continue reading To the first reviewer who kindly reviewed our article;

On behalf of my co-authors, I wish to thank you for taking the time out of your busy work schedule to read through our manuscript and make suggestions which only serve to strengthen the article overall and make it a better piece for submission. We would like to respond to each of the suggestions listed below.

Major changes include:

1. The authors agree that the extrapolation of EM changes in COL4A variants could be further elaborated on and have introduced a new section in the introduction looking at a Chinese cohort with both confirmed EM and COL4A mutations. EM is unlikely to be the preferred investigation for such cases, but if suggestive abnormalities are found by happenstance then investigation for a potential COL4A variant seems appropriate based on our current understanding. Unfortunately, due to small sample sizes, the sensitivity of such findings cannot be commented on at this time and forms a body of ongoing research into these potential links.

2. The authors have included a new figure (Figure 2) with a proposed worksheet for investigation into patients whom may present in this manner.

3. Unfortunately, the patients whom had EM abnormalities suggestive of a type IV collagen disorder have been lost to follow up, and the data on them is simply not able to extrapolated for this particular article (a shame as the authors were curious about such things too).

Minor changes include:

1. The title has been reworded to avoid confusion, and text within the paragraphs altered to reflect this change away from audit.

2. The Y axis in figure 1 is now labelled appropriately.

3. Tip and NOS, now specified within the manuscripts main paragraphs.

4. The authors thank the reviewers for pointing out small errors of inconsistency which can creep into a document like this, and have amended them appropriately

Again, many thanks for taking the time out to read through this manuscript.
To the first reviewer who kindly reviewed our article;

On behalf of my co-authors, I wish to thank you for taking the time out of your busy work schedule to read through our manuscript and make suggestions which only serve to strengthen the article overall and make it a better piece for submission. We would like to respond to each of the suggestions listed below.

Major changes include:

1. The authors agree that the extrapolation of EM changes in COL4A variants could be further elaborated on and have introduced a new section in the introduction looking at a Chinese cohort with both confirmed EM and COL4A mutations. EM is unlikely to be the preferred investigation for such cases, but if suggestive abnormalities are found by happenstance then investigation for a potential COL4A variant seems appropriate based on our current understanding. Unfortunately, due to small sample sizes, the sensitivity of such findings cannot be commented on at this time and forms a body of ongoing research into these potential links.

2. The authors have included a new figure (Figure 2) with a proposed worksheet for investigation into patients whom may present in this manner.

3. Unfortunately, the patients whom had EM abnormalities suggestive of a type IV collagen disorder have been lost to follow up, and the data on them is simply not able to extrapolated for this particular article (a shame as the authors were curious about such things too).

Minor changes include:

1. The title has been reworded to avoid confusion, and text within the paragraphs altered to reflect this change away from audit.

2. The Y axis in figure 1 is now labelled appropriately.

3. Tip and NOS, now specified within the manuscripts main paragraphs.

4. The authors thank the reviewers for pointing out small errors of inconsistency which can creep into a document like this, and have amended them appropriately

Again, many thanks for taking the time out to read through this manuscript.
Competing Interests: None to declare. Close
Report a concern
Respond or Comment

COMMENTS ON THIS REPORT

Author Response 07 Nov 2019

Justin Davis, Department of Nephrology, Barwon Health, Geelong, 3220, Australia

07 Nov 2019

Author Response

To the first reviewer who kindly reviewed our article;

On behalf of my co-authors, I wish to thank you for taking the time out of your busy work schedule ... Continue reading To the first reviewer who kindly reviewed our article;

On behalf of my co-authors, I wish to thank you for taking the time out of your busy work schedule to read through our manuscript and make suggestions which only serve to strengthen the article overall and make it a better piece for submission. We would like to respond to each of the suggestions listed below.

Major changes include:

1. The authors agree that the extrapolation of EM changes in COL4A variants could be further elaborated on and have introduced a new section in the introduction looking at a Chinese cohort with both confirmed EM and COL4A mutations. EM is unlikely to be the preferred investigation for such cases, but if suggestive abnormalities are found by happenstance then investigation for a potential COL4A variant seems appropriate based on our current understanding. Unfortunately, due to small sample sizes, the sensitivity of such findings cannot be commented on at this time and forms a body of ongoing research into these potential links.

2. The authors have included a new figure (Figure 2) with a proposed worksheet for investigation into patients whom may present in this manner.

3. Unfortunately, the patients whom had EM abnormalities suggestive of a type IV collagen disorder have been lost to follow up, and the data on them is simply not able to extrapolated for this particular article (a shame as the authors were curious about such things too).

Minor changes include:

1. The title has been reworded to avoid confusion, and text within the paragraphs altered to reflect this change away from audit.

2. The Y axis in figure 1 is now labelled appropriately.

3. Tip and NOS, now specified within the manuscripts main paragraphs.

4. The authors thank the reviewers for pointing out small errors of inconsistency which can creep into a document like this, and have amended them appropriately

Again, many thanks for taking the time out to read through this manuscript.
To the first reviewer who kindly reviewed our article;

On behalf of my co-authors, I wish to thank you for taking the time out of your busy work schedule to read through our manuscript and make suggestions which only serve to strengthen the article overall and make it a better piece for submission. We would like to respond to each of the suggestions listed below.

Major changes include:

1. The authors agree that the extrapolation of EM changes in COL4A variants could be further elaborated on and have introduced a new section in the introduction looking at a Chinese cohort with both confirmed EM and COL4A mutations. EM is unlikely to be the preferred investigation for such cases, but if suggestive abnormalities are found by happenstance then investigation for a potential COL4A variant seems appropriate based on our current understanding. Unfortunately, due to small sample sizes, the sensitivity of such findings cannot be commented on at this time and forms a body of ongoing research into these potential links.

2. The authors have included a new figure (Figure 2) with a proposed worksheet for investigation into patients whom may present in this manner.

3. Unfortunately, the patients whom had EM abnormalities suggestive of a type IV collagen disorder have been lost to follow up, and the data on them is simply not able to extrapolated for this particular article (a shame as the authors were curious about such things too).

Minor changes include:

1. The title has been reworded to avoid confusion, and text within the paragraphs altered to reflect this change away from audit.

2. The Y axis in figure 1 is now labelled appropriately.

3. Tip and NOS, now specified within the manuscripts main paragraphs.

4. The authors thank the reviewers for pointing out small errors of inconsistency which can creep into a document like this, and have amended them appropriately

Again, many thanks for taking the time out to read through this manuscript.
Competing Interests: None to declare. Close
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VERSION 2 PUBLISHED 26 Jul 2019

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Version 1 26 Jul 19	read	read

James McCaffrey, Cambridge University Hospitals NHS Foundation Trust, Cambridge, UK

Rachel Lennon, University of Manchester, Manchester, UK
Constantinos Deltas, University of Cyprus, Nicosia, Cyprus

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13 Nov 2019 | for Version 2

Rachel Lennon, Wellcome Trust Centre for Cell-Matrix Research, Faculty of Biology, Medicine and Health, University of Manchester, Manchester, UK

James McCaffrey, Department of Histopathology, Cambridge University Hospitals NHS Foundation Trust, Cambridge, UK

5 Views Cite this report Responses(0)

Approved

Competing Interests

No competing interests were disclosed.

We confirm that we have read this submission and believe that we have an appropriate level of expertise to confirm that it is of an acceptable scientific standard.

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16 Views

28 Oct 2019 | for Version 1

Constantinos Deltas, Molecular Medicine Research Center, Department of Biological Sciences, University of Cyprus, Nicosia, Cyprus

16 Views Cite this report Responses(1)

Approved

I believe there should be no renal biopsy attempted if it is not going to be studied fully by light microscopy, immunofluorescence studies and EM. It is an invasive procedure accompanied by some risk and when performed it must be fully exploited as very significant results may come up, which bear implications on treatment, inheritance patterns and family planning.
The authors found two patients who had undergone EM and there were findings indicative of a collagen IV disorder. They should have proceeded to genetic testing to verify this and strengthen their case. Even belatedly, this could be of benefit not only to the patients but also to family members.

Is the work clearly and accurately presented and does it cite the current literature?

Yes
Is the study design appropriate and is the work technically sound?

Yes
Are sufficient details of methods and analysis provided to allow replication by others?

Yes
If applicable, is the statistical analysis and its interpretation appropriate?

Not applicable
Are all the source data underlying the results available to ensure full reproducibility?

No source data required
Are the conclusions drawn adequately supported by the results?

Yes

Competing Interests

No competing interests were disclosed.

Reviewer Expertise

Human molecular and medical genetics, nephrogenetics, cell biology and biochemistry

I confirm that I have read this submission and believe that I have an appropriate level of expertise to confirm that it is of an acceptable scientific standard.

Respond to this report

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Author Response

07 Nov 2019

Justin Davis, Department of Nephrology, Barwon Health, Geelong, 3220, Australia

To the second reviewer who kindly reviewed our article;

On behalf of my co-authors, I wish to thank you for taking the time out of your busy work schedule to read through our manuscript and make suggestions which only serve to strengthen the article overall and make it a better piece for submission. We would like to ensure we have addressed both concerns pointed out below.

1. The authors agree with this point and feel most (if not all, at least in the case of native kidney) biopsies should be subjected to appropriate microscopic techniques which includes electron microscopy. It is unclear why some of our samples unfortunately were not, and remains a clear source of potential quality improvement that can be fed back, particularly when suggestive abnormalities may be found that influence further management.

2. The authors too are curious as to the genetic status of the two individuals who had suggestive EM changes noted within the body of the manuscript. Unfortunately on perusal of the data available they have been lost to follow up without a clear genetic diagnosis at this time.

Once again, many thanks for taking the time to review our work

View more View less

Competing Interests

None applicable.

Back to all reports

Reviewer Report

16 Views

18 Sep 2019 | for Version 1

James McCaffrey, Department of Histopathology, Cambridge University Hospitals NHS Foundation Trust, Cambridge, UK

Rachel Lennon, Wellcome Trust Centre for Cell-Matrix Research, Faculty of Biology, Medicine and Health, University of Manchester, Manchester, UK

16 Views Cite this report Responses(1)

Approved With Reservations

The authors could present a stronger argument for EM being the investigation of choice for identifying patients with COL4A mutations. Although the authors discuss the variable EM findings in patients with Alport syndrome or TBMN, this could be expanded. How sensitive are the GBM findings for COL4 mutations? Not all patients with COL4A variants have typical EM findings, and furthermore suggestive GBM abnormalities may be found in other genetic disorders including mutations in MYH9 and LMX1B.
There would be additional value if the authors could propose a pros/cons comparison of EM versus genetic screening with for example an NGS gene panel to identify these patients. This could be presented as a proposed workflow for investigation.
The authors identify 2 patients with EM findings suggestive of a COL4A disorder. However, there are no data detailing whether these patients did in fact harbour a COL4A variant (notably neither of them had haematuria at presentation). Are any data available, for example, on clinical response to immunosuppression, or any sequencing data?

Minor comments

The title described the study as an 'audit' but the text repeatedly refers to a 'retrospective cohort analysis.' As there are no defined standards against which clinical practice is being measured here, perhaps remove 'audit' from the title?
The Y-axis in Figure 1 should be labelled.
Which FSGS variant did the 2 patients with EM abnormalities fall into?
Improve consistency with nomenclature—use of conventional gene (COL4A) or protein names (type IV collagen).

Is the work clearly and accurately presented and does it cite the current literature?

Yes
Is the study design appropriate and is the work technically sound?

Yes
Are sufficient details of methods and analysis provided to allow replication by others?

Yes
If applicable, is the statistical analysis and its interpretation appropriate?

Yes
Are all the source data underlying the results available to ensure full reproducibility?

No source data required
Are the conclusions drawn adequately supported by the results?

Yes

Competing Interests

No competing interests were disclosed.

Reviewer Expertise

Paediatric nephrology, matrix biology, glomerular cell biology, histopathology

Respond to this report

Responses (1)

Author Response

07 Nov 2019

Justin Davis, Department of Nephrology, Barwon Health, Geelong, 3220, Australia

To the first reviewer who kindly reviewed our article;

On behalf of my co-authors, I wish to thank you for taking the time out of your busy work schedule to read through our manuscript and make suggestions which only serve to strengthen the article overall and make it a better piece for submission. We would like to respond to each of the suggestions listed below.

Major changes include:

1. The authors agree that the extrapolation of EM changes in COL4A variants could be further elaborated on and have introduced a new section in the introduction looking at a Chinese cohort with both confirmed EM and COL4A mutations. EM is unlikely to be the preferred investigation for such cases, but if suggestive abnormalities are found by happenstance then investigation for a potential COL4A variant seems appropriate based on our current understanding. Unfortunately, due to small sample sizes, the sensitivity of such findings cannot be commented on at this time and forms a body of ongoing research into these potential links.

2. The authors have included a new figure (Figure 2) with a proposed worksheet for investigation into patients whom may present in this manner.

3. Unfortunately, the patients whom had EM abnormalities suggestive of a type IV collagen disorder have been lost to follow up, and the data on them is simply not able to extrapolated for this particular article (a shame as the authors were curious about such things too).

Minor changes include:

1. The title has been reworded to avoid confusion, and text within the paragraphs altered to reflect this change away from audit.

2. The Y axis in figure 1 is now labelled appropriately.

3. Tip and NOS, now specified within the manuscripts main paragraphs.

4. The authors thank the reviewers for pointing out small errors of inconsistency which can creep into a document like this, and have amended them appropriately

Again, many thanks for taking the time out to read through this manuscript.

View more View less

Competing Interests

None to declare.

Alongside their report, reviewers assign a status to the article:

Approved - the paper is scientifically sound in its current form and only minor, if any, improvements are suggested

Approved with reservations - A number of small changes, sometimes more significant revisions are required to address specific details and improve the papers academic merit.

Not approved - fundamental flaws in the paper seriously undermine the findings and conclusions

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[2] 2. De Vriese AS, Sethi S, Nath KA, et al.: Differentiating Primary, Genetic, and Secondary FSGS in Adults: A Clinicopathologic Approach. J Am Soc Nephrol. 2018; 29(3): 759–74. PubMed Abstract | Publisher Full Text | Free Full Text

[3] 3. Gast C, Pengelly RJ, Lyon M, et al.: Collagen (COL4A) mutations are the most frequent mutations underlying adult focal segmental glomerulosclerosis. Nephrol Dial Transplant. 2016; 31(6): 961–70. PubMed Abstract | Publisher Full Text

[4] 4. Wickman L, Hodgin JB, Wang SQ, et al.: Podocyte Depletion in Thin GBM and Alport Syndrome. PLoS One. 2016; 11(5): e0155255. PubMed Abstract | Publisher Full Text | Free Full Text

[5] 5. Korstanje R, Caputo CR, Doty RA, et al.: A mouse Col4a4 mutation causing Alport glomerulosclerosis with abnormal collagen α3α4α5(IV) trimers. Kidney Int. 2014; 85(6): 1461–8. PubMed Abstract | Publisher Full Text | Free Full Text

[6] 6. Pierides A, Voskarides K, Athanasiou Y, et al.: Clinico-pathological correlations in 127 patients in 11 large pedigrees, segregating one of three heterozygous mutations in the COL4A3/ COL4A4 genes associated with familial haematuria and significant late progression to proteinuria and chronic kidney disease from focal segmental glomerulosclerosis. Nephrol Dial Transplant. 2009; 24(9): 2721–9. PubMed Abstract | Publisher Full Text

[7] 7. Malone AF, Phelan PJ, Hall G, et al.: Rare hereditary COL4A3/COL4A4 variants may be mistaken for familial focal segmental glomerulosclerosis. Kidney Int. 2014; 86(6): 1253–9. PubMed Abstract | Publisher Full Text | Free Full Text

[8] 8. Yao T, Udwan K, John R, et al.: Integration of Genetic Testing and Pathology for the Diagnosis of Adults with FSGS. Clin J Am Soc Nephrol. 2019; 14(2): 213–223, pii: CJN.08750718. PubMed Abstract | Publisher Full Text | Free Full Text

[9] 9. Hogan JJ, Mocanu M, Berns JS: The Native Kidney Biopsy: Update and Evidence for Best Practice. Clin J Am Soc Nephrol. 2016; 11(2): 354–62. PubMed Abstract | Publisher Full Text | Free Full Text

[10] 10. Xie J, Wu X, Ren H, et al.: COL4A3 mutations cause focal segmental glomerulosclerosis. J Mol Cell Biol. 2014; 6(6): 498–505. PubMed Abstract | Publisher Full Text

[11] 11. Kashtan CE: Alport syndrome. An inherited disorder of renal, ocular, and cochlear basement membranes. Medicine (Baltimore). 1999; 78(5): 338–60. PubMed Abstract | Publisher Full Text

[12] 12. Massella L, Onetti Muda A, Faraggiana T, et al.: Epidermal basement membrane alpha 5(IV) expression in females with Alport syndrome and severity of renal disease. Kidney Int. 2003; 64(5): 1787–91. PubMed Abstract | Publisher Full Text

[13] 13. Cai YI, Sich M, Beziau A, et al.: Collagen distribution in focal and segmental glomerulosclerosis: an immunofluorescence and ultrastructural immunogold study. J Pathol. 1996; 179(2): 188–96. PubMed Abstract | Publisher Full Text

[14] 14. Walker PD: The renal biopsy. Arch Pathol Lab Med. 2009; 133(2): 181–8. PubMed Abstract

[15] 15. Davis J: Davis et al FSGS biopsy audit.xlsx. figshare. Dataset. 2019.

[16] 16. Papazachariou L, Papagregoriou G, Hadjipanagi D, et al.: Frequent COL4 mutations in familial microhematuria accompanied by later-onset Alport nephropathy due to focal segmental glomerulosclerosis. Clin Genet. 2017; 92(5): 517–27. PubMed Abstract | Publisher Full Text

[17] 17. Lepori N, Zand L, Sethi S, et al.: Clinical and pathological phenotype of genetic causes of focal segmental glomerulosclerosis in adults. Clin Kidney J. 2018; 11(2): 179–90. PubMed Abstract | Publisher Full Text | Free Full Text

[18] 18. Sadowski CE, Lovric S, Ashraf S, et al.: A single-gene cause in 29.5% of cases of steroid-resistant nephrotic syndrome. J Am Soc Nephrol. 2015; 26(6): 1279–89. PubMed Abstract | Publisher Full Text | Free Full Text

[19] 19. Voskarides K, Pierides A, Deltas C: COL4A3/COL4A4 mutations link familial hematuria and focal segmental glomerulosclerosis. glomerular epithelium destruction via basement membrane thinning? Connect Tissue Res. 2008; 49(3): 283–8. PubMed Abstract | Publisher Full Text

[20] 20. Braunisch MC, Buttner-Herold M, Gunthner R, et al.: Heterozygous COL4A3 Variants in Histologically Diagnosed Focal Segmental Glomerulosclerosis. Front Pediatr. 2018; 6: 171. PubMed Abstract | Publisher Full Text | Free Full Text

The use of electron microscopy in the diagnosis of focal segmental glomerulosclerosis: are current pathological techniques missing important abnormalities in the glomerular basement membrane?

Abstract

Keywords

Revised Amendments from Version 1

Abbreviations

Introduction

Methods

Study background

Eligibility criteria

Sample treatment

Identification of participants and data analysis

Results

Participant information and diagnoses

Table 1. Demographic and clinical characteristics of the patients at baseline.

Use of electron microscopy

Figure 1. Annualised rate of biopsy samples subjected to electron microscopy.

Discussion

Figure 2. Proposed workflow for investigation of patients in this demographic.

Data availability

Underlying data

Author contributions

References

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