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Investigation of wine fermentation of three-leaf cayratia (Cayratia trifolia L.) using Saccharomyces cerevisiae 2.1

[version 4; peer review: 1 approved, 2 not approved]
PUBLISHED 27 Feb 2025
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Abstract

Background

Cayratia trifolia has been extensively studied for its bioactive components and medicinal properties. This study was carried out to evaluate the fermentation ability of Saccharomyces cerevisiae 2.1 yeast to determine suitable fermentation conditions.

Methods

The suitable initial sugar content, temperature, and incubation time were investigated in the fermentation in the 100-milliliter flask scale. Besides, a trial fermentation at the 1-liter scale was conducted to assess yeast performance.

Results

All treatments showed that the total sugar content decreased after 9 days of fermentation compared to the initial level. Temperature during fermentation has a direct effect on yeast activity. Temperature increases the growth of yeast and the speed of enzyme activity. The fermentation time changes depending on temperature, initial soluble solid contents, and yeast strains, leading to changes in the ethanol content after the end of fermentation. The results showed that the S. cerevisiae 2.1 was able to ferment at room temperature with an initial pH of 4.5, 24 °Brix, and a yeast density of 107 cells/mL. The appropriate fermentation conditions determined for S. cerevisiae 2.1 were 24 °Brix and incubation at room temperature (28-33°C) with a fermentation time of 11 days. In 1-L scale fermentation, three-leaf cayratia wine had 9.46% (v/v) and the fermentation efficiency was 90.34%. The wine produced had a unique color, flavor, and aroma that met the sensory evaluation of the Vietnamese national standard TCVN-3217:79.

Concusion

The results showed that the S. cerevisiae 2.1 was able to produce wine from Cayratia trifolia extract in suitable conditions with high ethanol content and organoleptic values. The results of this study supplied needed information for further studies on analysis of bioactive compounds produced by the fermentation.

Keywords

Cayratia trifolia, ethanol fermentation, Saccharomyces cerevesiae, three-leaf cayratia wine

Revised Amendments from Version 3

In this version, the abstract was modified so that the methods and conclusion included the most important information. In the abstract, information on the detailed volume of fermentations was displayed. Besides, the result and conclusion in the abstract were modified to include the important findings and expectations for further studies exploring the bioactive compounds in the wine fermentation of three-leaf cayratia.
The introduction section was edited to provide more neutral information on the benefits of moderate wine consumption to human health. In addition, the authors added some introduction of the background studies on wine fermentation using Cayratia trifolia to demonstrate the advantage of this fruit in food processing. This fortified the reasons why this study was conducted.
To supply the information on statistical analysis, Section 2.2.5 explained in detail how the data was analyzed.
Section 3.1 was added with some discussion on the effect of high and low sugar concentration on yeast growth and fermentation with corresponding references of ref 10 [doi:10.1093/femsyr/fov068] and ref 20 [doi: 10.3390/foods11101388]. In the first paragraph of Section 3.2, the reasonability of the optimal temperature discovered in this study was clarified with similar results from a report conducted by ref 24 [doi: 10.1002/j.2050-0416.1977.tb06813.x]. Moreover, the effects of longer fermentation time on higher ethanol production were linked with the supporting data from Table 3 in the second paragraph of Section 3.3. In Section 2.2.4 and Section 3.4, the methods and results of the sensory evaluation were shown since this is an important criterion for food production.
In the whole manuscript, the word 'fermentability' was changed to 'fermentation ability' for a clearer word choice. Besides, the authors rechecked and revised the manuscript's English writing.
The list of references was added with references 8, 9, 10, 20, and 24 to clarify information in this study.

See the authors' detailed response to the review by Javier Alonso-Del-Real
See the authors' detailed response to the review by Susan Abdul Raheem Hasan and Muazaz Azeez Hasan AL-Hadeethi

1. Introduction

In moderate consumption, wine, a traditional product of microbial fermentation, is proven to contribute benefits to human health and contains various bioactive ingredients. In the past, France and Italy were the cradles of the wine industry. Countries like Australia and New Zealand have created wine brands with modern technology and high quality. In the Asian market, particularly in Vietnam, wine has been conquering the consumer thanks to its abundant production, competitive price, and novel taste. Wines are fermented alcoholic beverages made from various base ingredients, such as apple, banana, papaya, mango, apricot, pineapple, and jackfruit juice. These are classified as grape wine, fruit wine, berry wine, vegetable wine, plant wine, and raisin wine, as well as flavors from flowers and herbs. Typical wine, natural wines (9–14% alcohol), or dessert and appetizer wines (15–21% alcohol) contain ethyl alcohol, sugar, acids, higher alcohols, tannins, aldehydes, esters, amino acids, minerals, vitamins, anthocyanins (Amerine et al., 1980).

Three-leaf cayratia (Cayratia trifolia) has been extensively studied for its bioactive components and medicinal properties. In particular, the abundant source distributed throughout the Mekong Delta of Vietnam gives the advantage for wine fermentation. Interestingly, C. trifolia antioxidant and polyphenolic compound contents were not significantly changed after fermentation (Doan et al., 2018d). Additionally, its color and flavor are specific to three-leaf cayratia wine, particularly proven in our previous studies (Doan et al., 2018d, 2019b), and C. trifolia berries are becoming an expected source of winemaking materials from grapes in Vietnam. However, the isolated thermotolerant yeast from C. trifolia berries is mainly used to ferment three-leaf cayratia wine. At the same time, non-thermotolerant S. cerevisiae is the primary source for winemaking at room temperature.

Currently, several studies were conducted for the fermentation of three-leaf Cayratia juice by S. cerevisiae which demonstrate for potential of wine fermentation using this fruit (Doan et al., 2022, 2024). Besides, yeast fermentation was proven to improve the effects of bio-active compounds including antioxidants in C. trifolia juice (Doan et al., 2022) by S. cerevisiae. According to Ngo et al. (2005), 50 yeast strains have been isolated from wine yeast starters in the Mekong Delta, of which S. cerevisiae 2.1 has the highest performance in fermentation. With these findings, the application of S. cerevisiae 2.1 in C. trifolia juice fermentation promises to create a wine with positive characteristics and quality.

Therefore, this study aimed to investigate and evaluate the wine fermentation of three-leaf cayratia juice by S. cerevisiae isolated from rice wine starters. In addition, 1 liter of C. trifolia juice was fermented to complete the larger-scale fermentation process of three-leaf cayratia wine.

2. Methods

2.1 Materials

Wine fermentation of three-leaf Cayratia (C. trifolia L.) using S. cerevisiae 2.1 was conducted between April 12th, 2022, and October 25th, 2022. Three-leaf cayratia samples were collected from shiny ripe, dark black, and undamaged berries in the Mekong Delta, Vietnam. The fresh berries were brought to the Laboratory of the Industrial Microbiology at the Institute of Food and Biotechnology, Can Tho University. The selected un-crushed ripe samples were washed several times with tap water, rinsed with distilled water, and squeezed out of flesh into juice to conduct further experiments.

S. cerevisiae 2.1 strain was isolated from a rice wine starter and stored at the Laboratory of Industrial Microbiology at the Institute of Food and Biotechnology Institute, Can Tho University (Ngo et al., 2005). A single colony of yeast was inoculated in Yeast extract - Peptone - Dextrose (YPD broth) (yeast extract 0.5%, peptone 0.5%, D-glucose 2.0%; sterilized at 121°C for 15 min) and shaken at 180 rpm at 30°C to 109 cells/mL of yeast inoculum level (using Haemocytometer – Neubauer).

2.2 Determination methods of three-leaf cayratia fermentation

2.2.1 Determination of initial sugar for three-leaf cayratia fermentation

Three-leaf cayratia juice was added with NaHSO3 (140 mg/L) and left alone for 2 h after being adjusted to 20, 24, and 28 °Brix (using sucrose provided by Bien Hoa Sugar Joint Stock Company) and pH 4.5 (Doan et al., 2018b, 2018d). One mL of S. cerevisiae was inoculated to 99 mL of three-leaf cayratia juice (107 cells/mL after inoculation) in a 250 mL Erlenmeyer flask. The flask was sealed with a water lock and incubated at room temperature (in triplicate). The changes in pH, °Brix, and ethanol contents were measured after 9 days of fermentation Nguyen (2007). The total sugar content of the fermentation process was analyzed according to Nielsen (2010), and fermentation efficiency was calculated.

Fermentation efficiency is calculated according to ethanol fermentation efficiency on the amount of sugar used, H (%) = (Et°*0.789*10)/(S*0.5111) where H: fermentation efficiency; Et°: alcohol content obtained (% v/v); 0.789: density of ethanol (0.789 g/cm3); 10: conversion factor; S: the actual amount of sugar used in glucose (following the phenol-sulfuric method); 0.5111: the grams of theoretical ethanol obtained from 1 gram of glucose.

2.2.2 Determination of temperature for three-leaf cayratia fermentation

Three-leaf cayratia juice was prepared with °Brix, selected from Section 2.2.1. After inoculation with 1 mL yeast culture, three-leaf cayratia juice was fermented at 25°C, room temperature (28–33°C), and 35°C for 9 days. The changes in pH, °Brix, and ethanol contents were determined as described above.

2.2.3 Determination of incubation time for three-leaf cayratia fermentation

Three-leaf cayratia juice was prepared with °Brix selected from the experiment as mentioned in Section 2.2.1, and incubated at the selected temperature from the experiment in Section 2.2.2, on days 7, 9, 11, and 13. The changes in pH, °Brix, and ethanol content were determined (see Section 2.2.1).

2.2.4 Trial fermentation in the 1-liter scale of the three-leaf cayratia wine

The initial sugar content, fermentation temperature, and fermentation time were determined based on the selective results of previous screening tests to ferment 1 liter of three-leaf cayratia juice (triplicate). Sensory evaluation of wine was based on the criteria of clarity, color, aroma, taste, and overall confidence according to Vietnamese National Standard 3217:79 (TCVN 3217:79), which is done through a sensory board consisting of 10 members at the Institute of Food and Biotechnology, Institute Can Tho University. The changes in pH, °Brix, ethanol content, and total sugar content were determined (see Section 2.2.1). The chemical and microbiological criteria were analyzed by the National Agro-Forestry Fisheries Quality Assurance Center - Branch 6 (Vietnam) following Vietnam National Standard QCVN 6-3:2010/BYT.

2.2.5 Statistical analysis

The analyzed data were processed using Excel 2013 (RRID:SCR_016137) (Microsoft Inc., USA). The variance and the Lease Significant Difference (LSD) were analyzed using SPSS Version 27.0.1.0 (RRID:SCR_002865) and Statgraphics Centurion XV - https://www.statgraphics.com/ (Manugistics Inc., USA).

3. Results

3.1 The initial sugar of the three-leaf cayratia fermentation using S. cerevisiae 2.1

Three-leaf cayratia fermentation processes were conducted with an initial pH of 4.5 and an initial sugar content of 20, 24, and 28 °Brix, respectively. As to the results, all treatments show that the total sugar contents decreased after 9 days of fermentation compared to the initial level ( Table 1). The ethanol content was the highest at 7.17% v/v in the 24 °Brix treatment, which is a significant difference compared to the 20 and 28 °Brix treatments. When the sugar concentration is too high, yeast fermentation is limited due to high osmotic pressure, lowering the alcoholic production (Kayikci & Nelsen, 2015). In principle, the rate of fermentation and the maximum amount of ethanol decreased when the sugar concentration was increased. However, a particular type of yeast strain can be selected, conditioned to grow at higher sugar concentrations (>30% sugar), and adapted (Matei & Kosseva, 2017). In contrast, at a lower pre-fermentation Brix concentration (20°Brix), ethanol production was limited at 6.23% (v/v) which may be due to the limit in carbon sources supply insufficient nutrients for yeast growth and fermentation (Timmermans et al., 2022).

Table 1. The effects of °Brix on the fermentation ability of S. cerevisiae 2.1.

Initial °BrixInitial sugar content (g/L)Used sugar content (g/L)Residual sugar content (g/L)Fermentation efficiency (%)Ethanol content (% v/v) at 20°C
20142.23116.6725.5682.47b6.23b
24187.98121.6366.3591.00a7.17a
28229.47115.60113.8775.13c5.64b

The total sugar content of the samples was determined using the phenol-sulfuric acid method (Nielsen, 2010), measured at a wavelength of λ = 490 nm. The linear regression equation was y = 0.0095x + 0.0049. The total sugar content after fermentation reflected the fermentation efficiency of S. cerevisiae 2.1. The 24 °Brix treatment had the highest fermentation conversion efficiency of 91.00% produced by S. cerevisiae 2.1, 82.47% at 20 °Brix treatment, and 75.13% at 28°Brix treatment, which was significantly different from 5% (p<0.05). The fermentation conversion efficiency was proportional to the ethanol content ( Table 2). The highest alcohol content (7.17%) was produced in the 24 °Brix treatment. S. cerevisiae strains are tolerant to low pH, high sugar content, and high ethanol concentrations compared to other species, thus reducing the risk of bacterial contamination during industrial fermentation (Nevoigt, 2008).

Table 2. The effects of incubation time on fermentation.

Temperature (°C)Initial °Brix °Brix after fermentation Ethanol content (% v/v) at 20°C
Room temp. (28–33°C)2412.176.94a
252414.176.16b
352416.834.28c

3.2 Temperature for three-leaf cayratia fermentation

The ethanol content was significantly different depending on the incubation temperature (Doan et al., 2019a). Temperature during fermentation has a direct effect on yeast activity. In this study, the ethanol content was produced at 6.94% (v/v) at room temperature, higher than that of 25°C (6.16% v/v) and 35°C (4.28% v/v). Based on a study, the optimum temperature for the growth and multiplication of S. cerevisiae is 30-35°C and is usually inhibited at temperatures higher than 39.8°C (Walsh & Martin, 1977).

Temperature increases the growth of yeast and the speed of enzyme activity. Because of the increased membrane fluidity, cell sensitivity to the toxic effects of alcohol increases with temperature. Thus, yeast viability may rapidly decline at temperatures above 20°C during wine fermentation. Normal cider production occurs at 20–25°C and lasts for 1 to 4 weeks (Waites et al., 2001).

3.3 Fermentation time for three-leaf cayratia wine

The fermentation time changes depending on temperature, initial soluble solid contents, and yeast strains, leading to changes in the ethanol content after the end of fermentation. The results showed the highest ethanol content at 11 days compared to 13, 9, and 7 days with 8.93%, 8.54%, 6.94%, and 6.23% v/v, respectively. There were no significant differences between 7 to 9 days and 11 to 13. However, these values were significantly different between days 7 and 11 ( Table 3).

Table 3. The fermentation temperature results of S. cerevisiae 2.1.

Incubation time (day)Initial °Brix °Brix after fermentation Ethanol content (% v/v) at 20°C
72414.176.94b
92412.506.23b
11249.338.93a
13249.338.54a

The end time of fermentation was inversely proportional to the degree of Brix after fermentation. Table 3 indicated the higher ethanol concentration of 8.54 % (v/v) and lower Brix of 9.33°Brix at the longest fermentation time at 13 days compared to the shorter times. The longer the time, the lower the °Brix, and the higher the ethanol concentration produced. Initially, when the ethanol concentration was quite low, the ethanol concentration began to increase, and the sugar content decreased.

3.4 Trial fermentation in the 1-liter scale of the three-leaf cayratia wine

The fermentation of fruit wine was tested with a volume of 1 liter, surveyed with a density of S. cerevisiae 2.1 at 107 cells/mL for 11 days at room temperature, and the juice was adjusted to pH 4.5 and 24 °Brix. From the above experiments, it can be seen that S. cerevisiae 2.1 produced an ethanol content of 9.49% (v/v), and the fermentation efficiency was quite high, reaching 90.34%.

After fermentation, the sensory properties were evaluated according to the criteria of TCVN 3217:79. Three-leaf wine was assessed as good in clarity and color, aroma, and taste, reaching 4.4, 4.2, and 3.8 points, respectively ( Figure 1). Sensory evaluation results show that the wine had no strange, cloudy smell, and the color and odor were specific to the product. Compared to the TCVN 3217:79, the sensory quality assessment of left-sided wine received a good rating.

b0b9c02a-2270-4ec5-8bb0-195eb29fc773_figure1.gif

Figure 1. Three-leaf cayratia wine and sensory evaluation chart.

The chemical and microbiological criteria of the final product were analyzed by the National Agro-Forestry Fisheries Quality Assurance Center - Branch 6, Vietnam. The results are shown in Table 4. These values are met to the Vietnam National Standard QCVN 6-3:2010/BYT.

Table 4. Quality evaluation of cayratia wine.

CriteriaVietnam National Standard
QCVN 6-3:2010/BYT
Method Result
Ethanol (% v/v)Ref.AOAC 972.11
(LOQ = 0.025%)
9.49
Methanol (mg/L)<400Ref.AOAC 972.11
(LOQ = 0.025%)
n.d.
Total microaerophile (CFU/mL)1,000ISO 4833-1:2013<10
Total yeast and mold (CFU/mL)100ISO 215227-2:2008<10
Coliforms (CFU/mL)0ISO 4832:20060
E. coli (CFU/mL)0ISO 16649-2:20010
Fecal Streptococci (CFU/mL)0ISO 7899-2:20000
Clostridium perfringens (CFU/mL)0ISO 7937:20040

4. Discussion

In a previous study, we isolated 151 strains of yeast from 53 samples of C. trifolia in the Mekong Delta. Of these, 30 isolates were highly fermentative and produced ethanol concentrations between 6.0 and 9.9% (v/v) (Doan et al., 2018c). The fermentation ability of thermotolerant yeast isolates from three-leaf juice showed that the survey with 3.6 of the natural pH, 22 °Brix by adding sucrose, the initial yeast density of 106 cells/mL, and 7 days of incubation time at 37°C. Saccharomyces sp. CT3.2 has a fast-filling time for the gas column in the Durham tube (after 12 h), and the ethanol content after fermentation was not high (5.4% v/v). In contrast, Saccharomyces sp. HG1.3 has a slower gas column filling time (after 18 h) but produces the highest ethanol content (9.9% v/v) (Doan et al., 2018b). Meanwhile, with initial fermentation conditions of pH 4.5, and 20 °Brix fermented at 35°C for 6 days, Saccharomyces sp. HG1.3 gave the ethanol content of post-fermentation three-leaf fruit wine 12.0% v/v (Doan et al., 2018d).

The fermentation ability of thermotolerant yeasts was investigated at pH 4.5, 22 °Brix, 107 cells/mL of the initial three-leaf juice, and at 37°C for 7 days. The ethanol contents of three-leaf wine produced by CM3.2, CM3.3, and BT1.2 were 8.95%, 7.01%, and 6.79% v/v, respectively (Torija et al., 2003; Doan et al., 2018a).

The fermentation of 1-liter results shows that the ethanol content met the Vietnam standard 6-3:2010/BYT of the Ministry of Health for wine (Vietnam National Standard 3217:79, 1979), which is also similar to fermentation studies on watermelon wine (Nguyen, 2010; Ngo et al., 2011), pineapple wine (Nguyen et al., 2013), and sim wine (Nguyen, 2010). However, the concentration value is lower than that of fruit wine fermented by a tolerant yeast strain isolated from fruit but aged at 35°C; for example, the strain S. cerevisiae AG2.1 is 11.36% (v/v) (Doan et al., 2018c), and S. cerevisiae CM3.2 is 12.46% v/v (Doan et al., 2019b).

The fermentation of 1 L results shows that the ethanol content meets the Vietnam Standard 6-3:2010/BYT of the Ministry of Health for wine, which is also similar to fermentation studies on watermelon wine (Ngo et al., 2011), pineapple wine (Nguyen et al., 2013), and sim wine (Nguyen, 2010). However, the concentration value is lower than that of fruit wine fermented by a tolerant yeast strain isolated from fruit but aged at 35 °C; for example, the strain S. cerevisiae AG2.1 is 11.36% (v/v) (Doan et al., 2018c), S. cerevisiae CM3.2 is 12.46% v/v (Doan et al., 2019b).

5. Conclusion

This study aimed to test the fermentative capacity of the S. cerevisiae 2.1 strain isolated from a starter on three-leaf cayratia juice. However, microbiological and physicochemical criteria have not yet been implemented. The results showed that the S. cerevisiae 2.1 strain was able to ferment at room temperature with an initial pH of 4.5, 24 °Brix, and yeast density of 107 cells/mL. Preliminary 1 liter of fermentation for 11 days produced an ethanol content of 9.49% (v/v). The chemical and microbiological criteria of final product was met to the Vietnam National Standard QCVN 6-3:2010/BYT.

5.1 Ethical considerations

This study was approved by the research group at Can Tho University and Can Tho University of Technology, Vietanm. All authors mentioned in the manuscript have agreed to authorship, read and approved the manuscript, and provided consent for submission and subsequent publication of the manuscript. The authors declare no conflicts of interest.

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Kieu Tien DT, Quoc Binh LD, Ngoc Mi HT et al. Investigation of wine fermentation of three-leaf cayratia (Cayratia trifolia L.) using Saccharomyces cerevisiae 2.1 [version 4; peer review: 1 approved, 2 not approved]. F1000Research 2025, 12:312 (https://doi.org/10.12688/f1000research.129075.4)
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Version 4
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PUBLISHED 27 Feb 2025
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Reviewer Report 02 Sep 2025
Shatha Abdullah Allaith, University of Kerbala Faculty of Agriculture (Ringgold ID: 125663), Kerbala, Karbala Governorate, Iraq 
Not Approved
VIEWS 5
1. The experimental design is not up to the required scientific standard, especially since it used a yeast isolate from a previous study but did not utilize it well to demonstrate the importance of the current study.
2. In ... Continue reading
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Allaith SA. Reviewer Report For: Investigation of wine fermentation of three-leaf cayratia (Cayratia trifolia L.) using Saccharomyces cerevisiae 2.1 [version 4; peer review: 1 approved, 2 not approved]. F1000Research 2025, 12:312 (https://doi.org/10.5256/f1000research.178047.r408091)
NOTE: it is important to ensure the information in square brackets after the title is included in all citations of this article.
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Reviewer Report 17 Mar 2025
Javier Alonso-Del-Real, Instituto de Biomedicina de Valencia, Valencia, Spain;  Instituto de Agroquimica y Tecnología de Alimentos, Paterna, Spain 
Not Approved
VIEWS 10
Although authors address some of the concerns raised before, the paper still contain some important issues that must be reviewed.

Authors claim that moderate consumption of wine is healthy. As I pointed out in the previous round, ... Continue reading
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Alonso-Del-Real J. Reviewer Report For: Investigation of wine fermentation of three-leaf cayratia (Cayratia trifolia L.) using Saccharomyces cerevisiae 2.1 [version 4; peer review: 1 approved, 2 not approved]. F1000Research 2025, 12:312 (https://doi.org/10.5256/f1000research.178047.r368792)
NOTE: it is important to ensure the information in square brackets after the title is included in all citations of this article.
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Reviewer Report 05 Mar 2025
Muazaz Azeez Hasan AL-Hadeethi, Botany, University of Baghdad, Baghdad, Baghdad, Iraq 
Susan Abdul Raheem Hasan, Biology, University of Baghdad, Baghdad, Iraq 
Approved
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I have ... Continue reading
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Hasan AL-Hadeethi MA and Raheem Hasan SA. Reviewer Report For: Investigation of wine fermentation of three-leaf cayratia (Cayratia trifolia L.) using Saccharomyces cerevisiae 2.1 [version 4; peer review: 1 approved, 2 not approved]. F1000Research 2025, 12:312 (https://doi.org/10.5256/f1000research.178047.r368791)
NOTE: it is important to ensure the information in square brackets after the title is included in all citations of this article.
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Reviewer Report 22 Jun 2024
Susan Abdul Raheem Hasan, Biology, University of Baghdad, Baghdad, Iraq 
Muazaz Azeez Hasan AL-Hadeethi, Botany, University of Baghdad, Baghdad, Baghdad, Iraq 
Approved with Reservations
VIEWS 27
The article (Investigation of wine fermentation of three-leaf cayratia (Cayratia trifolia L.) using Saccharomyces cerevisiae 2.1) investigates the optimal conditions for the fermentation process. It discusses each condition and whether it was appropriate to meet the best efficiency (the aim ... Continue reading
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Raheem Hasan SA and Hasan AL-Hadeethi MA. Reviewer Report For: Investigation of wine fermentation of three-leaf cayratia (Cayratia trifolia L.) using Saccharomyces cerevisiae 2.1 [version 4; peer review: 1 approved, 2 not approved]. F1000Research 2025, 12:312 (https://doi.org/10.5256/f1000research.163527.r289555)
NOTE: it is important to ensure the information in square brackets after the title is included in all citations of this article.
  • Author Response 27 Feb 2025
    Huynh Xuan Phong, Can Tho University, Vietnam
    27 Feb 2025
    Author Response
    1. Method in abstract missing details such as (volumes, weights, etc.) that were tested, which then were cleared in the main part of the manuscript.
    This is a valuable comment. ... Continue reading
COMMENTS ON THIS REPORT
  • Author Response 27 Feb 2025
    Huynh Xuan Phong, Can Tho University, Vietnam
    27 Feb 2025
    Author Response
    1. Method in abstract missing details such as (volumes, weights, etc.) that were tested, which then were cleared in the main part of the manuscript.
    This is a valuable comment. ... Continue reading
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Reviewer Report 06 May 2024
Javier Alonso-Del-Real, Instituto de Biomedicina de Valencia, Valencia, Spain;  Instituto de Agroquimica y Tecnología de Alimentos, Paterna, Spain 
Not Approved
VIEWS 26
The present paper aims to describe the suitability of a particular yeast strain isolated in a previous related work for the fermentation of Cayratia trifolia fruits. To that purpose authors conduct several fermentation experiments with different amount of initial sugars ... Continue reading
CITE
CITE
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Alonso-Del-Real J. Reviewer Report For: Investigation of wine fermentation of three-leaf cayratia (Cayratia trifolia L.) using Saccharomyces cerevisiae 2.1 [version 4; peer review: 1 approved, 2 not approved]. F1000Research 2025, 12:312 (https://doi.org/10.5256/f1000research.163527.r263138)
NOTE: it is important to ensure the information in square brackets after the title is included in all citations of this article.
  • Author Response 27 Feb 2025
    Huynh Xuan Phong, Can Tho University, Vietnam
    27 Feb 2025
    Author Response
    1. First of all, some of the conclusions presented are not supported by the results or the experiments conducted. Also, The reader may have trouble finding the link between some ... Continue reading
COMMENTS ON THIS REPORT
  • Author Response 27 Feb 2025
    Huynh Xuan Phong, Can Tho University, Vietnam
    27 Feb 2025
    Author Response
    1. First of all, some of the conclusions presented are not supported by the results or the experiments conducted. Also, The reader may have trouble finding the link between some ... Continue reading

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Approved - the paper is scientifically sound in its current form and only minor, if any, improvements are suggested
Approved with reservations - A number of small changes, sometimes more significant revisions are required to address specific details and improve the papers academic merit.
Not approved - fundamental flaws in the paper seriously undermine the findings and conclusions
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