Diagnostic utility of mast cell analysis in hypoplastic bone marrows for differentiation of aplastic anaemia from hypoplastic myelodysplastic neoplasm: A retrospective study

Introduction

Haematological disorders characterised by hypocellular marrows like aplastic anaemia (AA) and hypocellular myelodysplastic neoplasm (MDS-h) are diagnostic challenges for haematologists as AA, MDS-h, other immune cytopenias, paroxysmal nocturnal haemoglobinuria (PNH), and inherited bone marrow (BM) failure disorders are all considered BM failure states with significant clinicopathological overlap.^1–3 However, the differentiation is critical as both AA and MDS-h have different management protocols with varying outcomes and prognoses. Compared to patients with AA, those with MDS-h have shorter median survival rates and exhibit a lower response to immunosuppressive therapy.^4,5

AA is defined as pancytopenia in the peripheral blood secondary to BM hypocellularity and is a diagnosis of exclusion.⁶ MDS is a diverse collection of clonal disorders that affect the hematopoietic stem cells (HSCs) and are characterised by cytopenias of varying degrees in the peripheral blood and inefficient haematopoiesis with BM dysplasia. Myelodysplasias with hypocellular BM (MDS-h) comprises around 10–15% of all MDS cases and are characterized by BM hypocellularity. Both AA and MDS-h share a common pathophysiological pathway with CD34 positive progenitor cells being fundamental in the pathogenesis of both these entities. CD34 positive HSCs are the target of autoimmune attack in AA making its count significantly decreased in the BM of AA. Whereas CD34 progenitor cells are the cells from which MDS originates. This explains the elevated quantity of CD34 positive cells in the BM of patients with MDS as a result of neoplastic clonal expression.³

Recent studies have proposed an additional mechanism exploring the possibility of the role of BM niche in the pathogenesis of AA. The BM microenvironments also known as the stem cell niche comprises endosteal cells, macrophages, fat cells, fibroblasts, mast cells and microvascular endothelial cells.⁷ The stem cell microenvironment transmits signals to maintain the fundamental features of the HSCs such as the ability to self-regenerate and the capacity to reproduce all the lineages. Thus, alteration in the BM niche was considered as a potential cause of hematopoietic impairment; however, the ways in which these changes affect the development of the disease remains unclear.⁸

Several researchers have studied the correlation between the BM microenvironment and dysregulated haematopoiesis. Alterations in the components of the microenvironment in the BM, including lymphocytes, mast cells, macrophages, and stromal cells, may be associated with the impaired haematopoiesis leading to AA and/or myeloproliferative neoplasms. The quantitative increase of mast cells in AA cases has been demonstrated in several studies and has resulted in the hypothesis that mast cells can cause cytotoxic effects on the hematopoietic cells in the marrow.⁸

Mast cells originate from BM progenitor cells and migrate into various tissues to complete their maturation. BM mast cell quantification is proposed as a supplementary diagnostic and prognostic tool to differentiate AA from other BM failure conditions like MDS-h.⁵

The percentage of mast cells in the BM is generally less than 1% of all the nucleated cells in the marrow and is distributed singly.⁹ Mast cells stain metachromatically with Toluidine blue (TB), which stains the granules of mast cells purple to red. Histological staining with TB is a highly effective, rapid, direct and antibody-free technique to detect mast cells in tissue sections.^10,11 Mast cells have longer life spans and are not directly targeted by the autoimmune attack on the stem cell compartment, resulting in the relative increase in its number in the BM of patients with AA. The quantitative increase in mast cell is clonal in mastocytosis and benign in acquired AA. However, its nature is not completely understood in AA and MDS-h.^12,13

In AA, lower mast cell count is related to better prognosis, but no such relation exists in MDS.⁵ Several researchers have shown increased numbers of mast cells in cases of AA and have proposed an association between the increased mast cells in the BM with the poor outcome of the patients.^14,15 Irrespective of the involved mechanism, a surge in the quantity of mast cells is assumed to be a factor responsible for the reduced cellularity observed in AA and MDS-h.^16,17 Therefore, the present study aimed to evaluate the diagnostic utility of mast cell quantification in hypoplastic marrow (HM) to distinguish AA from MDS-h. Furthermore, the association of increased mast cells with decreased HSC in the BM of such entities was analysed.

Methods

Results

This study included a total of 65 cases of hypoplastic and aplastic marrows that met the inclusion and exclusion criteria, of which 21 (32.3%) cases were AA, 12 cases were MDS-h (18.5%) and 32 (49.2%) cases were diagnosed as HM.

A bimodal peak was noticed in the age distribution pattern with the majority of the population being from the paediatric and adolescent age group (32.3%) and the second peak comprising of people above 60 years of age (28.7%). The rest of the cases belonged to the age group of 20-60 years old. Slight male predilection was noted with M: F ratio being 1.6:1. Overall, 38% (25) of the total population were female and 62% (40) of the population were male. The demographic data of the individual cases are available as Underlying data.²⁵

Mast cells in the BM were highlighted by staining them with TB. Mast cells were counted amongst 200 nucleated cells. More than 1% of mast cells were seen in 36 (55.4%) of the total cases. Seven (10.8%) cases showed more than 10% of mast cells. Mast cell count of more than 1% was considered as increased. Increased numbers of mast cells were observed in most of the cases of AA (31, 48%) followed by HM (20, 31%), and MDS-h (14, 21%), while the rest of the cases showed less than 1% of mast cells in their marrow. The Chi squared test was done with respect to the increased number of mast cells and the different entities of AA, MDS-h and other causes of HM was significant (P: 0.040). The distribution of the increased mast cells amongst the various differentials in the contingency table is depicted in Table 1. Metachromatically stained mast cells in light microscopy in a case of AA and MDS-h are depicted in Figure 1 and Figure 2, respectively. These microphotographs are annotated and minimally cropped. The original unprocessed microphotographs are available as Underlying data.²⁵

Figure 1. Aplastic anaemia in 37 year old female patient.

A: In low power view aplastic marrow is seen with cellularity less than 10% in the marrow spaces (magnification, 20×, H&E). B: Toluidine blue special staining in low power view shows metachromatically stained mast cells (magnification, 20×, Toluidine blue staining). C: Toluidine blue special staining in high power view shows six metachromatically stained mast cells in around 20-22 nucleated cells (magnification, 40×, Toluidine blue staining).

Figure 2. Hypocellular myelodysplastic neoplasm in 16 year old male patient.

Toluidine blue special staining in high power view shows four metachromatically stained mast cells in around 200 nucleated cells (magnification, 40×, Toluidine blue staining).

CD34 IHC expression was assessed to calculate HSCs and categorised as more than and less than 0.5% of positive cells (decreased) amongst all the hematopoietic cells of the marrow. Decreased expression was noted in 45 (69.2%) of the cases.

An increased number of mast cells was seen in 36 (55.45%) cases of the total study population while significantly decreased/absent HSCs were seen in 45 (69.2%) cases. A total of 25 (69.4%) of the cases that showed an increased number of mast cells also showed significantly decreased HSCs. Whereas 26 (57.7%) of the cases that had decreased HSCs showed an increased number of mast cells. However, the association between decreased HSCs and increased mast cells was statistically insignificant (Chi squared test, P: 0.10). The contingency table of the same is depicted in Table 2.

Discussion

BM failure conditions with marrow that is hypocellular for age embodies a broad spectrum of acquired and inherited conditions. Patients with MDS-h are known to have a worse prognosis than those with AA because they are more prone to neoplastic progression.¹⁸

Haematologists still continue to struggle in making a precise diagnosis due to the blurred lines between AA, other mimicking conditions like MDS-h and the various inherited BM failure syndromes. Identifying the exact disorder leading to a HM is critical as the cause of the disease significantly affects the choice of therapy.^19,20

BM evaluation, comprising of both trephine biopsy and aspirate, is obligatory to ascertain the diagnosis. There is convincing evidence that these discrete haematological conditions share a conjoint pathophysiological pathway centred at the alteration and/or damage of the hematopoietic stem and progenitor cells (HSPCs) by the cytotoxic effect of T cells. Increased T cells produce excessive proinflammatory cytokines (interferon-γ and tumor necrosis factor-α), leading to the reduced proliferation and increased apoptosis of the HSPCs.²¹

The distinction between AA and MDS-h is mostly dependent on the histomorphological and IHC features of the BM but in HMs it is challenging due to the scarcity of the hematopoietic cells present in the BM and the overlapping of the cytomorphological features of both the entities.⁴ Erythroid dysplasia is not uncommon in AA and thus cannot be used as a sole distinguishing feature.

Mast cell quantification in the BM was performed by several researchers and a common finding of increased number of mast cells in HMs was observed.^14–16,22 In our study we considered more than 1% of mast cells per 200 nucleated cells as the cut off for defining increase in the quantity of mast cells. The results in our study were similar to other studies with elevated mast cells seen in 55.4% of the total cases out of which the majority of the cases were AA (P<0.05). The quantity of mast cells ranged from 0-100 mast cells per 200 nucleated cells. The case with the highest number of mast cells belonged to the category of AA. However, the variation in the quantity of mast cells in both AA and MDS-h lies in the same range. This is consistent with the findings of Ingrid Fohlmeister²² done on 48 cases of different forms of MDS and 59 cases of AA. They demonstrated that there was overlap in the number of mast cells found in the BM of individuals with AA and MDS-h. The number of mast cells varied from 0-205/mm².

Complex interactions between the hematopoietic cells and the BM niche occur during haematopoiesis, although the exact cause and mechanism involved in the deregulated haematopoiesis is unknown. HSCs reside in a specialized microenvironment (niche) in the BM. BM microenvironment is important in maintaining the function of HSC as it is thought to transmit signals sustaining key HSC properties like regenerating capability and multilineage reproducing capacity. Thus, alteration in the BM niche can be a potential factor associated with hematopoietic impairment. However, it is still unclear how these changes contribute to the development of the disease.

In cases of idiopathic AA, the ineffective haematopoiesis can be considered as a result of some unknown changes in the BM microenvironment. Mast cells are considered by some researchers as a cause of the ineffective haematopoiesis in AA as it stimulates apoptosis and auto immune attack by cytotoxic killer cells. Studies have demonstrated that the BM of patients with AA shows increases in the quantity of mast cells and natural killer (NK) cells reflecting the cytotoxic or immune-mediated damage of the marrow.^8,11 Our study strengthens this hypothesis as 17 (48%) of the cases that showed an increased number of mast cells belonged to the patients with AA. Thus, increases in the numbers of mast cells can be considered as a causative factor for the insufficient haematopoiesis. However, in our study the association between increased mast cells and reduced HSCs was statistically insignificant.

Patients with AA may have altered BM niches that contribute to the disease pathophysiology or cause ineffective haematopoiesis. Quantification of mast cell can help in identifying the prognosis of the cases of AA. Reduced numbers of mast cells are a good prognostic indicator in AA while no such relation is observed in cases of MDS.¹⁶

Although hematopoietic stem cell transplantation plays a frontline role in the treatment of haemato-oncological conditions, there are still significant issues that need to be resolved, including the difficulty in finding matched donors and the ineffective engraftment of HSCs into the BM. An improved comprehension of the interactions amongst each component of the BM niche can help in identifying the potential therapeutic targets in cases of BM failure as well as myeloproliferative disorders.²³

Simulating the hematopoietic niche is a promising method for effectively increasing the number of HSPCs and fine-tuning their features ex vivo. The creation of a functioning hematopoietic microenvironment in vitro is still constrained by attempts to replicate it without a thorough grasp of the specific roles played by individual components involved in the hematopoietic setting. Detailed knowledge of the crucial cells in the BM microenvironment and the way they affect the regulation of HSCs can aid in establishing an ex vivo structural arrangement for the expansion of HSCs to provide a novel source of therapeutic blood cells for haematological disorders.^23,24

In conclusion, mast cell quantification in the BM biopsies with the help of TB special stain can act as a supplementary tool to distinguish the two entities as mast cells are increased in the majority of the cases of AA, while it is increased only in a few cases of MDS-h. The knowledge of the association between the increased number of mast cells with decreased HSCs can help in establishing an ex vivo environment mimicking the normal BM. This can aid in cultivating HSPCs for therapeutic purposes as well as in identifying the targets for targeted therapies in disorders like AA and other haematological malignancies.