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differentiation, epigenetics, eye development, single-cell ATAC-seq, single-cell RNA-seq
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Watabe Y, Kobayashi S, Nakayama T et al. Expression status transition of NOTCH1 accompanies chromatin remodeling in human early retinal progenitor cells [version 2; peer review: 2 approved with reservations]. F1000Research 2025, 14:31 (https://doi.org/10.12688/f1000research.159630.2)
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Brief Report
Revised
Expression status transition of NOTCH1 accompanies chromatin remodeling in human early retinal progenitor cells
[version 2; peer review: 2 approved with reservations]
PUBLISHED 22 Sep 2025
OPEN PEER REVIEW
REVIEWER STATUS
This article is included in the Japan Institutional Gateway gateway.
Abstract
Corresponding author:
Masaharu Yoshihara
Competing interests:
No competing interests were disclosed.
Grant information:
This study was supported by the JSPS KAKENHI Grant-in-Aid for Early-Career Scientists (grant no. JP23K14429) to M.Y.
The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
Copyright:
© 2025 Watabe Y et al.
This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
How to cite: Watabe Y, Kobayashi S, Nakayama T et al. Expression status transition of NOTCH1 accompanies chromatin remodeling in human early retinal progenitor cells [version 2; peer review: 2 approved with reservations]. F1000Research 2025, 14:31 (https://doi.org/10.12688/f1000research.159630.2)
First published: 06 Jan 2025, 14:31 (https://doi.org/10.12688/f1000research.159630.1)
Latest published: 22 Sep 2025, 14:31 (https://doi.org/10.12688/f1000research.159630.2)
The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
Revised Amendments from Version 1
Thank you so much for reading our paper. We have made significant improvements as suggested by the two reviewers (Dr. Mariko Kashiwagi and Dr. Zhongjie Tang). Among the many improvements inspired by the reviewers’ insightful suggestions, the particularly important ones are broadening the genome region analysis (100 kb for both 5’ upstream and 3’ downstream regions of the gene encoding region), clarifying of the hierarchy of the four NOTCH receptor expressions, and analyzing the binding motifs for the key transcription factors (LHX2, PAX6, RAX and VSX2). The details of the changes in each section of the revised manuscript are described below.
In Abstract:
We have newly mentioned probable NOTCH3 involvement in retinal progenitor cell (RPC) differentiation. We have also included a summary of motif analyses for transcription factors important for early RPCs in chromatin remodeling regions in the NOTCH1 locus.
In Introduction:
We have included biological context of NOTCH3 in RPC differentiation for the purpose of clarifying the significance of NOTCH3 examination in this study.
In Methods:
We have described the detail of motif analyses for LHX2, PAX6, RAX and VSX2.
In Results:
For all the three data (day 59, 74 and 78), we have updated cell cluster names, expanded genomic ranges of chromatin accessibility analyses, and conducted the motif analyses. For visibility, we have separated the main figure for each day sample into two (e.g., Figure 1 and 2 for day 59). The details of cell clusters, chromosome regions of ATAC peaks, and motif analyses are supplementarily provided as csv or xlsx files. Moreover, we have significantly revised the text, corresponding to updated data and clarification of the hierarchy of observed NOTCH expressions.
In Discussion:
We have discussed epigenetic regulations of the four NOTCH genes, while addressing the discrepancy between mRNA expression and chromatin accessibility of NOTCH2 and NOTCH4.
To read any peer review reports and author responses for this article, follow the "read" links in the Open Peer Review table.
Introduction
Signal transduction depends on the expression of receptors regulated at multiple levels. These regulations include chromatin remodeling and DNA-binding proteins, such as transcription factors and transcriptional repressors. Because fine-tuned signal transduction is necessary for development, it is important to clarify the regulatory mechanisms of receptor expression for understanding the regulation of cell differentiation and subsequent tissue development.
Notch signaling in mammals is dependent on the binding of five canonical DSL ligands and four Notch receptors.1 It has been suggested that Notch loci are subject to chromatin remodeling under both normal2–7 and pathological8–16 conditions. In terms of developmental biology, the retina is a good model for investigating cellular differentiation involving Notch signaling.17 The developed retina is composed of multiple cell types, including retinal ganglion cells, bipolar cells, photoreceptors, amacrine cells, horizontal cells, and Müllar glial cells, all of which originate from the retinal progenitor cells (RPCs). RPC is characterized by the expression of Lhx2, Pax6, Rax and Vsx2 while there are some additional marker genes for developing horizontal cells/retinal ganglion cells (Onecut1/2),18 developing amacrine cells (Elavl2/4),19 developing photoreceptors/amacrine cells/Müllar glial cells (Eef1a1),20 developing retinal ganglion cells (Meis2),21 developing horizontal cells (Lhx1, Ptf1a),22 and glial cells (Pax2).23 Of the four Notch receptors in mammals, Notch1 has been suggested to be involved in the maintenance of RPCs and differentiation into retinal ganglion cells.24 In addition to Notch1, Notch3 expression has also been suggested in the early RPCs, which potentially affects the amount and oscillation of total Notch signaling via its susceptibility to the receptor cleavage and subsequent signal transduction.25 However, it is unclear when and how Notch receptor expression is switched on and off in RPCs during early differentiation. Here, we re-analyzed a public multi-omics dataset of single-cell RNA-seq and single-cell ATAC-seq from three human fetal retinas26 to clarify the timing of Notch receptor expression and examine the involvement of chromatin remodeling in this receptor expression switch.
Methods
A single-cell multi-omics dataset (GSE183684)26 was downloaded from the Gene Expression Omnibus (https://www.ncbi.nlm.nih.gov/geo/); data from days 59, 74 and 78 in the dataset were selectively used because they contained many undifferentiated retinal progenitor cells. The data were processed in Seurat version 5.1.027 and Signac version 1.14.028 pipelines in R version 4.4.1 on the Ubuntu 22.04.4 LTS environment. Pseudotime analysis and integration of the single-cell RNA-seq data and the single-cell ATAC-seq data was conducted by using the “FindTransferAnchors” function of Signac and Monocle3 version 1.3.7,29 respectively. The conditions used in the analysis are provided in the GitHub repository. We searched the transcription factor binding motifs for LHX2 (TAATTA),30 PAX6 (CGCCTGA),31,32 RAX ([C/T]AATTA)33,34 and VSX2 (TAATT [A/G])35 (“Supplementary_table_motif.xlsx”) manually by referencing human genome assembly hg38 in the University of California Santa Cruz genome browser (https://genome.ucsc.edu/).
Results
Re-analysis day 59 human fetal retina
First, early gestational stage samples were characterized (day 59). Uniform manifold approximation and projection (UMAP) analysis of single-cell RNA-seq identified 14 clusters (Figure 1A), which were further characterized by marker gene expressions (Figure 1B). This sample primarily contained RPCs with various differentiation statuses except for PAX2-expressing glial cells. We defined LHX2, PAX6, RAX and VSX2 as early markers. We considered that early markers-expressing RPCs and MKI67-expressing RPCs constitute early RPCs. In addition, pseudotime analysis suggested that RPCs decreased expressions of LHX2, PAX6, RAX and VSX2 during differentiation into either ONECUT1/ 2-, ELAVL2/4- or EEF1A1-expressing RPCs (Figure 1C). The details of each cluster are available in “Supplementary_table_count.xlsx” in our GitHub repository. Next, we examined Notch mRNA expressions and found that NOTCH1, NOTCH2 and NOTCH3 were expressed primarily in early RPCs with NOTCH1 showing the highest expression, which prompted us to focus on NOTCH1 and NOTCH3,25 whereas NOTCH4 expression was barely detectable (Figure 1D). Then, we re-analyzed single-cell ATAC-seq data, which was mathematically integrated with its single-cell RNA-seq data by using the “FindTransferAnchors” function of Signac (Figure 1E). In the early RPCs (Early markers-expressing RPC1- 3_d59 and MKI67-expressing RPC1- 2_d59), we observed multiple peaks in the 100 kb upstream and downstream chromosome regions of NOTCH1, some of which were diminished in the other RPC clusters such as ELAVL2/4- or EEF1A1-expressing RPCs (Figure 2A, arrowheads). Since these regions contained multiple binding motifs for LHX2, PAX6, RAX and VSX2 (Figure 2A, arrowheads numbered 1-3) (“Supplementary_table_motif.xlsx”), chromatin remodeling of these regions possibly regulates NOTCH1 expression transition during RPC differentiation although the expression transitions of LHX2, PAX6, RAX and VSX2 may also support the NOTCH1 expression transition (Figure 2A). Since NOTCH3 has also been suggested for early RPC differentiation,25 we also conducted chromatin accessibility analysis of NOTCH3 and observed less prominent chromatin remodeling in the 100 kb upstream and downstream regions spanning the NOTCH3 locus and the mRNA diminishment supports the previous report25 (Figure 2B). The tables of the chromosome regions of each variable peak in NOTCH1 and NOTCH3 are provided in our GitHub repository as “ATAC_peaks_NOTCH1_d59.csv” and “ATAC_peaks_NOTCH3_d59.csv”, respectively. Although NOTCH2 and NOTCH4 have been less documented for RPC differentiation in the literature, we have provided the dot plots of the Notch expressions and the coverage plots of NOTCH2 and NOTCH4 in “Additional_file_1.tif” in our GitHub repository. In contrast to NOTCH2 mRNA expression in the early RPCs (Figure 1D), we observed several peaks in the chromosome region spanning the NOTCH2 locus although it was difficult to point out the peak regions specific for the early RPCs (Additional_file_1C). Conversely, in contrast to little NOTCH4 mRNA expression (Figure 1D), we found that multiple peaks were constantly open in the NOTCH4 locus during RPC differentiation (Additional_file_1D, arrowheads), some of which contained multiple binding motifs for LHX2, PAX6, RAX and VSX2 (Additional_file_1D, arrowheads numbered 1-2) (“Supplementary_table_motif.xlsx”). The table of the chromosome regions of each variable peak in NOTCH4 is provided in our GitHub repository as “ATAC_peaks_NOTCH4_d59.csv.”
Figure 1. Notch mRNA expression decrease in the day 59 sample.
UMAP analysis of the single-cell RNA-seq data identified 14 clusters. (B) Dot plot of marker genes. (C) Monocle3 pseudotime analysis for clarifying the differentiation status. (D) Feature plot of NOTCH1-4. Note that NOTCH1, NOTCH2 and NOTCH3 expressions were prominent in the early RPC clusters. (E) UMAP analysis of the single-cell ATAC-seq data, which was integrated with the single-cell RNA-seq data.
Figure 2. Concomitant chromatin remodeling of NOTCH1 and NOTCH3 in the day 59 sample.
The coverage plot of the 100 kb upstream and downstream chromosome regions of NOTCH1. The upstream of the genes is on the right. Variable peak regions are indicated by arrowheads, with the regions containing transcription binding motifs numbered as 1-3. (B) The coverage plot of the 100 kb upstream and downstream chromosome regions of NOTCH3. The upstream of the genes is on the right. A variable peak region which contains transcription binding motifs is indicated by an arrowhead numbered as 1.
Re-analysis day 74 human fetal retina
To validate the changes in Notch mRNA expressions and chromatin accessibility in the Notch loci during RPC differentiation, we investigated another early gestational stage sample (day 74). UMAP analysis identified 15 clusters (Figure 3A) that were further characterized by marker gene expressions (Figure 3B). This sample contained RPCs with various differentiation statuses. Similar to day 59 sample, pseudotime analysis suggested that the early RPCs differentiated into either ELAVL2/4, ONECUT1/MEIS2 or ONECUT1/2-expressing RPCs (Figure 3C). ONECUT1/MEIS2-expressing RPCs differentiated into RPCs that markedly expressed VSX2. Next, we examined Notch mRNA expressions and found that NOTCH1, NOTCH2 and NOTCH3 were expressed primarily in early RPCs with NOTCH1 showing the highest expression whereas NOTCH4 expression was barely detectable (Figure 3D), similar to the day 59 sample. We then re-analyzed the single-cell ATAC-seq data, which were integrated with the single-cell RNA-seq data (Figure 3E). In the early RPCs (Early markers-expressing RPC1- 4_d74 and MKI67-expressing RPC_d74), we observed multiple peaks in the 100 kb upstream and downstream chromosome regions of NOTCH1, some of which were diminished in the other RPC clusters, such as ELAVL2/4- or ONECUT1/2-expressing RPCs (Figure 4A, arrowheads). Since these regions contained multiple binding motifs for LHX2, PAX6, RAX and VSX2 (Figure 4A, arrowheads numbered 1-4) (“Supplementary_table_motif.xlsx”), chromatin remodeling of these regions possibly regulates NOTCH1 expression transition during RPC differentiation although the expression transitions of LHX2, PAX6, RAX and VSX2 may also support the NOTCH1 expression transition (Figure 4A). Since NOTCH3 has also been suggested for early RPC differentiation,25 we also conducted chromatin accessibility analysis of NOTCH3 and observed less prominent chromatin remodeling in the 100 kb upstream and downstream regions spanning the NOTCH3 locus (Figure 4B). The tables of the chromosome regions of each variable peak in NOTCH1 and NOTCH3 are provided in our GitHub repository as “ATAC_peaks_NOTCH1_d74.csv” and “ATAC_peaks_NOTCH3_d74.csv”, respectively. In summary, similar to the day 59 sample, in the day 74 sample, a concomitant mRNA decrease and chromatin remodeling in the NOTCH1 locus were observed. Although NOTCH2 and NOTCH4 have been less documented for RPC differentiation in the literature, we have provided the dot plots of the Notch expressions and the coverage plots of NOTCH2 and NOTCH4 in “Additional_file_2.tif” in our GitHub repository. In contrast to NOTCH2 mRNA expression (Figure 4D), we observed a single peak in the NOTCH2 locus for EEF1A1-expressing RPC_d74, and therefore, no peak regions in the NOTCH2 locus specific for the early RPCs (Additional_file_2C). Converesly, in contrast to little NOTCH4 mRNA expression (Figure 4D), we found that multiple peaks were constantly open in the NOTCH4 locus during RPC differentiation (Additional_file_2D, arrowheads), some of which contained multiple binding motifs for LHX2, PAX6, RAX and VSX2 (Additional_file_2D, arrowheads numbered 1-3) (“Supplementary_table_motif.xlsx”). The table of the chromosome regions of each variable peak in NOTCH4 is provided in our GitHub repository as “ATAC_peaks_NOTCH4_d74.csv.”
Figure 3. Notch mRNA expression decrease in the day 74 sample.
(A) UMAP analysis of the single-cell RNA-seq data identified 15 clusters. (B) Dot plot of marker genes. (C) Monocle3 pseudotime analysis. (D) Feature plot of NOTCH1-4. Note that NOTCH1, NOTCH2 and NOTCH3 expressions were prominent in the early RPC clusters. (E) UMAP analysis of the single-cell ATAC-seq data which was integrated with the single-cell RNA-seq data.
Figure 4. Concomitant chromatin remodeling of NOTCH1 and NOTCH3 in the day 74 sample.
(A) The coverage plot of the 100 kb upstream and downstream chromosome regions of NOTCH1. The upstream of the gene is on the right. Variable peak regions are indicated by arrowheads, with the regions containing transcription binding motifs numbered as 1-4. (B) The coverage plot of the 100 kb upstream and downstream chromosome regions of NOTCH3. The upstream of the gene is on the right. Variable peak regions are indicated by arrowheads, with the regions containing transcription binding motifs numbered as 1-2.
Re-analysis day 78 human fetal retina
To further confirm the developmental changes in Notch mRNA expression and chromatin accessibility at the Notch loci, we added one more early gestational stage sample (day 78). UMAP analysis identified 18 clusters (Figure 5A) that were further characterized by marker gene expressions (Figure 5B). The sample contained RPCs with various differentiation statuses. Pseudotime analysis suggested that the early RPCs differentiated into either ONECUT1/2-expressing RPCs, which later differentiated into PTF1A and LHX1-expressing RPCs, ONECUT1/MEIS2-expressing RPCs, or ELAVL2/4-expressing RPCs (Figure 5C). Next, we examined Notch mRNA expressions and found that NOTCH1, NOTCH2 and NOTCH3 were expressed primarily in early RPCs with NOTCH1 showing the highest expression whereas NOTCH4 expression was barely detectable (Figure 5D), similar to the day 59 and 74 samples. We then re-analyzed the single-cell ATAC-seq data, which was integrated with the single-cell RNA-seq data (Figure 5E). In the early RPCs (Early markers-expressing RPC1-3_d78 and MKI67-expressing RPC1-2_78), we observed multiple peaks in the 100 kb upstream and downstream chromosome regions of NOTCH1, some of which were diminished in the other RPC clusters (Figure 6A, arrowheads). Since these regions contained multiple binding motifs for LHX2, PAX6, RAX and VSX2 (Figure 6A, arrowheads numbered 1-4) (“Supplementary_table_motif.xlsx”), chromatin remodeling of these regions possibly regulates NOTCH1 expression transition during RPC differentiation although the expression transitions of LHX2, PAX6, RAX and VSX2 may also support the NOTCH1 expression transition (Figure 6A). Since NOTCH3 has also been suggested for early RPC differentiation,25 we also conducted chromatin accessibility analysis of NOTCH3 and observed less prominent chromatin remodeling in the 100 kb upstream and downstream regions spanning the NOTCH3 locus (Figure 6B). The tables of the chromosome regions of each variable peak in NOTCH1 and NOTCH3 are provided in our GitHub repository as “ATAC_peaks_NOTCH1_d78.csv” and “ATAC_peaks_NOTCH3_d78.csv”, respectively. In summary, examinations of all the three independent samples suggested that NOTCH1 mRNA expression decreased as RPC differentiation progresses, which was concomitant with chromatin remodeling in the NOTCH1 locus. Importantly, chromatin remodeling regions contained multiple transcription factor binding motifs. Although NOTCH2 and NOTCH4 have been less documented for RPC differentiation in the literature, we have provided the dot plots of the Notch expressions and the coverage plots of NOTCH2 and NOTCH4 in “Additional_file_3.tif” in our GitHub repository. In contrast to NOTCH2 mRNA expression (Figure 5D), we observed several peaks in the chromosome regions spanning the NOTCH2 locus although it was difficult to point out peak regions specific for the early RPCs (Additional_file_3C). Conversly, in contrast to little NOTCH4 mRNA expression (Figure 5D), we found that multiple peaks were constantly open in the NOTCH4 locus during RPC differentiation (Additional_file_3D, arrowheads), some of which contained multiple binding motifs for LHX2, PAX6, RAX and VSX2 (Additional_file_3D, arrowheads numbered 1-3) (“Supplementary_table_motif.xlsx”). The table of the chromosome regions of each variable peak in NOTCH4 is provided in our GitHub repository as “ATAC_peaks_NOTCH4_d78.csv.”
Figure 5. Notch mRNA expression decrease in the day 78 sample.
(A) UMAP analysis of the single-cell RNA-seq data identified 18 clusters. (B) Dot plot of marker genes. (C) Monocle3 pseudotime analysis. (D) Feature plot of NOTCH1- 4. Note that NOTCH1, NOTCH2 and NOTCH3 expressions were prominent in early RPC clusters. (E) UMAP analysis of the single-cell ATAC-seq data which was integrated with the single-cell RNA-seq data.
Figure 6. Concomitant chromatin remodeling of NOTCH1 and NOTCH3 in the day 78 sample.
(A) The coverage plot of the 100 kb upstream and downstream chromosome regions of NOTCH1. The upstream of the gene is on the right. Variable peak regions are indicated by arrowheads, with the regions containing transcription binding motifs numbered as 1-4. (B) The coverage plot of the 100 kb upstream and downstream chromosome regions of NOTCH3. The upstream of the gene is on the right. Variable peak regions are indicated by arrowheads, with the regions with transcription binding motifs are numbered as 1-2.
Discussion
The involvement of Notch signaling in cell fate choices is well documented, including in Drosophila neurogenesis36 and mammalian biliary development.37 Although the regulation of Notch receptor expression is necessary for these processes, to the best of our knowledge, few studies have used genome-wide investigations of the underlying molecular mechanisms. To examine chromatin remodeling in such regulatory mechanisms, we re-analyzed a single-cell RNA-seq and ATAC-seq dataset from developing retinas in which differentiation trajectories were well characterized. By re-analyzing three independent samples, we observed chromatin remodeling in the NOTCH1 locus, which contains multiple binding motifs for transcription factors, concomitant with changes in its mRNA expression during RPC differentiation. These findings highlight that the fine-tuned regulation of Notch receptor expressions occurs at an epigenetic level.
NOTCH1 and NOTCH3 were well documented in the early RPC differentiation compared to NOTCH2 and NOTCH4. Indeed, we observed NOTCH1 and NOTCH3 mRNA expressions in contrast to little NOTCH4 mRNA expression throughout the three samples. We observed comparable NOTCH2 mRNA expression in the early RPCs although we are unable to fully explain the discrepancy between mRNA expression and chromatin accessibility regarding this gene owing to lack of significant peak regions in the NOTCH2 locus (Additional_file_1C, 2C and 3C). Some possible causes such as regulation via distal enhancer and technical limitations of ATAC-seq may exist. When NOTCH2 mRNA is transcribed in support with those mechanisms, the decrease of NOTCH2 mRNA expression during RPC differentiation may be influenced by a post-transcriptional mechanism since a small non-coding regulatory RNA (CAT1) reportedly promotes stabilization of Notch2 mRNA.38 The roles and regulations of NOTCH2 are, however, beyond the scope of this study. Conversely, we observed little NOTCH4 mRNA expression and its high chromatin accessibility with multiple binding motifs for LHX2, PAX6, RAX and VSX2. This observation might be owing to the absence of other transcription factors for NOTCH4 mRNA expression since this gene expression has been suggested to highly depend on the cell type and its expressing transcription factors. For example, Notch4 is limitedly expressed in endothelial cells, compared to Notch1, under regulation of AP-1 transcription factor.39 Although we are unable to provide solid conclusions on the expression and regulation of NOTCH2 and NOTCH4 in the early RPCs, we believe that this does not impede our findings that NOTCH1 mRNA expression transition during RPC differentiation accompanied chromatin remodeling.
An ophthalmological study revealed that the epigenetic landscape of cell type-specific enhancers shifted during differentiation of RPCs.40 For example, in the single-cell ATAC-seq data from embryonic day 14.5 mouse retina, motif enrichment for Lhx2, Rax and Pax6 in the early RPCs were observed, and footprinting analysis validated binding of those transcription factors to their motifs. These high chromatin accessibilities decreased as they differentiated into retinal ganglion cells and non-retinal ganglion cells. Although that study is excellent in providing comprehensive and in-depth insights, ours is unique in focusing on the Notch loci for clarifying the regulatory mechanisms in view of Notch signaling biology.
Finally, we note that further investigations, such as a large deletion of these regions, will be needed to evaluate the contribution of the identified chromatin remodeling to the differential expression of Notch receptors in RPC subsets.
Authors’ contributions
Y. W.: Data Curation, Formal Analysis, Software, Visualization, Writing – Original Draft Preparation
S.K.: Writing – Original Draft Preparation
T.N.: Writing – Original Draft Preparation
S.T.: Supervision, Writing – Review & Editing
M.Y.: Conceptualization, Formal Analysis, Funding Acquisition, Methodology, Project Administration, Software, Writing – Original Draft Preparation
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CITE
how to cite this article
Watabe Y, Kobayashi S, Nakayama T et al. Expression status transition of NOTCH1 accompanies chromatin remodeling in human early retinal progenitor cells [version 2; peer review: 2 approved with reservations]. F1000Research 2025, 14:31 (https://doi.org/10.12688/f1000research.159630.2)
NOTE: If applicable, it is important to ensure the information in square brackets after the title is included in all citations of this article.
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Version 1
VERSION 1
PUBLISHED 06 Jan 2025
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How to cite this report:
Tang Z. Reviewer Report For: Expression status transition of NOTCH1 accompanies chromatin remodeling in human early retinal progenitor cells [version 2; peer review: 2 approved with reservations]. F1000Research 2025, 14:31 (https://doi.org/10.5256/f1000research.175389.r398458)
The direct URL for this report is:
https://f1000research.com/articles/14-31/v1#referee-response-398458
https://f1000research.com/articles/14-31/v1#referee-response-398458
NOTE: it is important to ensure the information in square brackets after the title is included in this citation.
Reviewer Report 22 Aug 2025
Zhongjie Tang,
University of Southern California, Los Angeles, Southern California, USA
Approved with Reservations
VIEWS 12
Evaluation
This study presents a reanalysis of publicly available single-cell multi-omics datasets to explore the relationship between NOTCH1 expression dynamics and chromatin accessibility during early stages of human retinal progenitor cell (RPC) differentiation. The authors apply standard single-cell RNA-seq ... Continue reading
This study presents a reanalysis of publicly available single-cell multi-omics datasets to explore the relationship between NOTCH1 expression dynamics and chromatin accessibility during early stages of human retinal progenitor cell (RPC) differentiation. The authors apply standard single-cell RNA-seq ... Continue reading
CITE
HOW TO CITE THIS REPORT
Tang Z. Reviewer Report For: Expression status transition of NOTCH1 accompanies chromatin remodeling in human early retinal progenitor cells [version 2; peer review: 2 approved with reservations]. F1000Research 2025, 14:31 (https://doi.org/10.5256/f1000research.175389.r398458)
The direct URL for this report is:
https://f1000research.com/articles/14-31/v1#referee-response-398458
https://f1000research.com/articles/14-31/v1#referee-response-398458
NOTE: it is important to ensure the information in square brackets after the title is included in all citations of this article.
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24
How to cite this report:
Kashiwagi M. Reviewer Report For: Expression status transition of NOTCH1 accompanies chromatin remodeling in human early retinal progenitor cells [version 2; peer review: 2 approved with reservations]. F1000Research 2025, 14:31 (https://doi.org/10.5256/f1000research.175389.r359501)
The direct URL for this report is:
https://f1000research.com/articles/14-31/v1#referee-response-359501
https://f1000research.com/articles/14-31/v1#referee-response-359501
NOTE: it is important to ensure the information in square brackets after the title is included in this citation.
Reviewer Report 05 Feb 2025
Approved with Reservations
VIEWS 24
Remarks to the Author:
In this manuscript, the authors investigated whether NOTCH family genes (NOTCH1–4) were expressed in retinal progenitor cells (RPCs) and whether changes in chromatin accessibility at promoter regions contributed to their transcriptional activation. To ... Continue reading
In this manuscript, the authors investigated whether NOTCH family genes (NOTCH1–4) were expressed in retinal progenitor cells (RPCs) and whether changes in chromatin accessibility at promoter regions contributed to their transcriptional activation. To ... Continue reading
CITE
HOW TO CITE THIS REPORT
Kashiwagi M. Reviewer Report For: Expression status transition of NOTCH1 accompanies chromatin remodeling in human early retinal progenitor cells [version 2; peer review: 2 approved with reservations]. F1000Research 2025, 14:31 (https://doi.org/10.5256/f1000research.175389.r359501)
The direct URL for this report is:
https://f1000research.com/articles/14-31/v1#referee-response-359501
https://f1000research.com/articles/14-31/v1#referee-response-359501
NOTE: it is important to ensure the information in square brackets after the title is included in all citations of this article.
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