Keywords
Antioxidant enzymes, Faradarmani, Consciousness Field, Salt stress, Wheat
This article is included in the Plant Science gateway.
Antioxidant enzymes, Faradarmani, Consciousness Field, Salt stress, Wheat
Some words were edited throughout the text.The two words including Fara-darmani and Fara-therapist were replaced with Faradarmani and Faradarmangar in the figures and the main text.
See the authors' detailed response to the review by František Hnilička
See the authors' detailed response to the review by Halimeh Hassanpour
Most criticism about complementary therapy is the lack of scientific research. In order to be accepted, academic studies using different study designs are necessary. Since one of the critical objections occurring when human beings are treated with complementary therapy is the placebo responses, biochemical plant-based studies can be a suitable method to clarify the phenomenon (Betti et al., 2003). Among the different plant model systems, the wheat plant has been repeatedly selected for homeopathy research Baumgartner et al. (2000) showed that homeopathic drugs improved plant resistance, which exerted their effect through detoxification processes.
In arid and semi-arid areas of the world, salinity is considered as a major factor in reducing crop productivity (Poonia et al., 1972). Plant growth is adversely affected by multiple environmental stresses, including biotic (e.g. fungi, bacteria, viruses, herbivores) and abiotic (e.g. low temperature, salt, drought, heavy metal toxicity). Among these the salination of arable land is one of the key factors that threatens the sustainability of the agricultural industry. Thus, many studies have attempted to explore processes that contribute to plant survival under salt stress (Ashraf & Harris, 2004) as a strategy to improve productivity and fertility. It is well documented that plants that are exposed to biotic or abiotic stresses have biochemical changes that exert oxidative damage through Reactive Oxygen Species (ROS) (Smirnoff, 1993). These free radicals disrupt cell membrane stability by peroxidation of polyunsaturated fatty acids in the plant cell membranes (Bor et al., 2003; Hernández & Almansa, 2002; Shalata et al., 2001) and denature protein and nucleic acids (Chen et al., 1993). To alleviate adverse effects of oxidative stress, plants have developed diverse strategies, which are categorized as enzymatic, such as catalase, superoxide dismutase (SOD), peroxidase (POX), polyphenol oxidase (PPO) and ascorbate peroxidase, and non-enzymatic that directly scavenge ROS, such as glutathione, tocopherol, flavonoids and ascorbates (Agarwal & Pandey, 2004). Plants that have developed an antioxidant system that participates in ROS scavenging have better resistance to oxidative damage (Parida & Das, 2005).
To date, there have been many studies to explore the relationship between the intangible and physical world, especially the interaction between the human mind and outside physical world. For instance, it has been reported that the mind can affect dice tosses (Rhine, 1944). Researchers have previously focused on probabilistic systems, like tossing coins, using random number generators (RNGs). The first RNG study was conducted by Radin & Nelson (1989), which included 597 experiments and 235 control studies. This type of research was considered as ‘micro-psychokinesis’ (micro-PK) (Jahn et al., 1980; Varvoglis & Bancel, 2015). However, micro-PK is not completely acceptable to science because of the null effects and failure to replicate previous positive results (Jahn et al., 2000). Throughout history, studies can be found that explain the interaction between the human mind and body, such as ‘distant healing’, or the effects of the mind on inanimate physical systems, like morphological changes in a thin strip of metal (Randall & Davis, 1982).
Faradarmani is one of the many Consciousness Field (CFs) founded by Mohammad Ali Taheri. In this theoretical concept, cosmic consciousness is the collection of consciousness, wisdom or intelligence governing the world of existence, which is also called ‘Awareness’. Consciousness, according to Taheri, is one of the three existing elements of the universe apart from matter and energy. By defining Consciousness as neither matter nor energy, we cannot associate a quantity to it. Since consciousness isn’t measurable, its existence can only be known through experience (Taheri, 2013). According to this theory, any living creature, including animals and plants, may be cured via humans by “Etesal” (virtual connecting) to internet-like facilities called the Cosmic Consciousness Network (CCN). In this type of affection, mind-matter interaction occurs through connecting to the CCN by a Faradarmangar (the person who makes the virtual connection). Faradarmani establishes a consciousness bond between the ‘whole’ consciousness and the ‘parts’ where all constituents will be scanned and corrected (Taheri, 2013). Although the mechanism of this linkage is not yet definable by science, its consequences can be measured and studied scientifically.
The aim of this study was to determine the effects of Faradarmani CF on alleviating the effects of salt stress in a spring wheat variety (Star).
Subjects of study received Faradarmani via Faradarmangar by ‘announcement’ which is a process in which Taheri or any Faradarmangar (announcer) declares and sends the information of the subjects under study (e.g. the number of groups) to the CCN. The influence of Consciousness Field begins with the connection between the human mind and the CCN. In other words, the Faradarmangar’s mind acts as an intermediary between the subject of the study and the CCN. The first author of this study is an announcer and at the same time as the seedlings were subjected to salt stress, two groups of treatments received Faradarmani (group 2: 0 mM NaCl and group 4: 150 mM NaCl). This exposure occurs without any kind of physical intervention, since consciousness according to Taheri’s concepts is neither matter nor energy, receiving this treatment is possible from close and far distances.
This experiment can easily be repeated by any researcher even from far distances by registering on the COSMOintel website (the Assign Announcement section) COSMOintel is a research center, under the supervision of the innovator of the method (Mohammad Ali Taheri) that has been established to design and implement repeatable and reproducible studies in the world of science1.
In this research, we used a spring wheat variety Triticum aestivum L. var Star (Seed and Plants Improvement Institute, Karaj, Alborz Province). Seeds were surface sterilized with 2.5% sodium hypochlorite for 10 min and washed thoroughly with sterile distilled water. After sterilization, seeds were soaked in distilled water for 24 hours at room temperature. For each treatment three pots were prepared and six seeds were initially sown in plastic pots (10 × 10 cm) containing perlite soil. After the germination they were thinned to five plants per pot. First, all pots were irrigated daily with 100ml distilled water for four days. Then received 100ml half-strength Hoagland’s nutrient solution (pH= 5.7) (Hoagland & Arnon, 1950) every other day for another 12 days (chemicals purchased from Sigma-Aldrich).
The sixteen-day-old seedlings were treated with salinity. The salts were added to the nutrient solution. To prevent osmotic shock, salt stress was started gradually on 50 mM NaCl (100ml). Every other day the concentration was increased by 50mM until 150 mM was attained. Salt stress was continued for three weeks (150mM NaCl was added every other day). Initial Faradarmani connection treatment occurred at the same time as adding the first NaCl solution. Four treatment groups (n=3 pots/group) were performed as follows: group 1, control – grown with no NaCl and did not receive Faradarmani CF; group 2 –grown with no NaCl and did receive Faradarmani CF; group 3 – treated with 150 mM NaCl for three weeks and did not receive Faradarmani CF treatment; group 4 – treated with 150 mM NaCl for three weeks and did receive Faradarmani CF treatment.
After three weeks, four fully expanded leaves were picked per replicate for future analyses. They were frozen in liquid N2 and transferred to -20˚C for imminent bench experiments.
For measuring photosynthetic pigments, we used the method by Arnon (1949). 0.5 gram of fresh leaf material placed in acetone 80% and homogenized to extract chlorophyll. The resulting solution was filtered through Whatman’s No.1 filter paper. After extracting of photosynthetic pigments in acetone 80%, absorbance of chlorophyll a and b was recorded by UV-visible spectrophotometer (Shimadzu UV-160) at 645 and 663 nm respectively. According to Arnon (1949) chlorophyll concentrations were calculated using the formulas below:
Chl.a (mg l-1) = [12.7 (A663) – 2.69 (A645)] * 0.5 ml of extracted sample
Chl.b (mg l-1) = [22.9 (A645) – 4.69 (A663)] * 0.5 ml of extracted sample
Total chlorophyll = Chl a + Chl b
Measurement of the hydrogen peroxide (H2O2) content was performed according to Velikova et al. (2000). One gram of leaf tissue was homogenized on ice with 5 ml of trichloroacetic acid (TCA; Sigma-Aldrich) 0.1% (w/v) and centrifuged at 12000 rpm for 15 min. Subsequently, 0.5 ml of 10 mM potassium phosphate buffer (pH 7) and 1 ml of 1M potassium iodide was added to 0.5 ml of supernatant. The absorbance of supernatant was determined at 390 nm wavelength.
Malondialdehyde (MDA), which is a product of lipid peroxidation, has been considered as an indicator of membrane destruction. MDA content was determined according to Stewart & Bewley (1980). We added 5 ml of TCA to 0.2 g of fresh leaf. After homogenization, the solution was centrifuged at 13000 × g for 10 min. The mixture of 1 ml of supernatant with 4 ml of 0.5% thiobarbituric acid in 20% TCA was heated for 30 min at 95˚C and quickly placed in an ice bucket. Subsequently, we centrifuged the solution at 10000 × g for 10 minutes and recorded the absorbance of supernatant at 532 and 600 nm. The calculation of MDA was done from the extinction coefficient of 155 mM-1 cm-1.
To determine enzyme activity, 0.1 g of fresh third leaves were ground in 3 ml of 50 mM Tris-HCl buffer (pH 6.8) at 4˚C. The homogenate was centrifuged at 13000 × g for 20 min at 4˚C. The supernatants were then collected and stored at -70˚C for determination of enzymes activity.
SOD activity was determined using the assay system described by Giannopolitis & Ries (1977). The reaction mixture consisted of 50 mM phosphate buffer pH 7.5, 13 mM methionine, 0.1 mM Na-EDTA, 75µM NBT, 75 µM Riboflavin and 100 µL of enzyme extract in a final volume of 3 ml (all the chemicals were purchased from Sigma-Aldrich). The mixture in glass test tubes was placed 30 cm from 30 W fluorescent lamps. Identical solutions without illumination and enzyme extract were considered as blanks. Since SOD has the ability to inhibit the photochemical reduction of nitroblue tetrazolium (NBT), the amount of inhibition was estimated by reducing the generation of color in the presence of light. One unit of SOD was described as the amount of enzyme that lead to 50% inhibition of NBT reduction. After 16 min, the absorbance at 560 nm was recorded against the blank. SOD activities were calculated as units per milligram of protein.
POX activity was measured based on the method of Abeles & Biles (1991). The activity of POX was estimated by adding 0.01 ml of enzyme extract to 4 ml of 0.2 M acetate buffer (pH 5), 0.4 ml H2O2 (3%), 0.2 ml 20 mM benzidine. The absorbance was recorded at 530 nm using spectrophotometer and POX activity was expressed as U mg-1 protein.
PPO was assayed according to Raymond et al. (1993). The reaction solution contained 2.5 ml of 200 mM sodium phosphate buffer (pH 6.8), 0.2 ml of 20 mM pyrogallol and 0.01 ml enzyme extract. The temperature of the reaction mixture was 40˚C. The changes in absorbance were recorded at 430 nm.
Salinity decreased the contents of chlorophyll (Chl) a, Chl b and total Chl (Figure 1a–c). Under the influence of Faradarmani CF with 150 mM NaCl, the contents of total Chl, Chl a and Chl b were elevated (34.8%, 17.8% and 169%, respectively) compared to the plants treated with 150mM without Faradarmani CF.
Effects of Faradarmani Consciousness Field treatment on (a) chlorophyll a, (b) total chlorophyll, (c) chlorophyll b, (d) hydrogen peroxide (H2O2). Plants were treated with 0 mM NaCl (control) or 150mM NaCl Vertical bars indicate mean ± standard error of three replicates. Means followed by the same letter were not significantly different at P<0.05.
The effect of NaCl treatment on H2O2 is shown in (Figure 1d). Results of the present study showed that H2O2 content remained unchanged under salinity condition whereas for the Faradarmani CF treated groups (control and 150mM NaCl) showed significant enhancement 100% and 57.1%, respectively.
MDA content was assessed as an oxidative indicator. Salinity stress caused an increase of 59.5% in MDA content as compared to that of control. The Faradarmani CF treatment to the salt-stressed plant decreased MDA content by about 12.5% (Figure 2d).
(a) polyphenol oxidase (PPO), (b) peroxidase (POX), (c) superoxide dismutase (SOD) and (d) malondialdehyde (MDA) content. Plants were treated with 0 mM NaCl (control) or 150mM NaCl Vertical bars indicate mean ± standard error of three replicates. Means followed by the same letter were not significantly different at P<0.05.
POX activity was significantly increased by NaCl treatment up to 244 % compared with control while under salinity treatment exposure to Faradarmani CF decreased the activity of enzyme by 34 % (Figure 2b).
SOD activity was slightly increased under salinity. However, it was found that with Faradarmani CF the activity of SOD in salinity condition was about 220 % higher than that in salinity without Faradarmani CF treatment (Figure 2c)
Similarly, PPO activity was not significantly higher than non-saline condition (control). However, the PPO activity showed an increase of 168% under salinity in response to Faradarmani CF compared to the salinity treated without Faradarmani CF treatment (Figure 2a).
In this study, chlorophyll a and b, and total chlorophyll contents decreased remarkably under salinity conditions (Figure 1). This is supported by previous data reported in tomato plants (Al-aghabary et al., 2005) and wheat (Ashraf et al., 2002), where salt stress unfavorably affects chlorophyll content. The decrease in chlorophyll content might be due to the formation of ROS in salinity stress that leads to lipid peroxidation and damages thylakoid membranes (Mittler, 2002). Our results showed that under salinity treatment, Faradarmani CF ameliorated the adverse effects of salt stress, probably by improving antioxidant systems, scavenging ROS and increasing the chlorophyll a and b contents (Figure 1). Similar findings were presented by screening the effect of the Static Magnetic Field (SMF) on pigments in Lettus and it has been reported that all photosynthetic pigments, especially chlorophyll a increased under the influence of SMF and this positive result was associated with improvement of antioxidant system (Latef et al., 2020).
For many years, numerous investigations have been made into salinity stresses and attempt to alleviate detrimental effects of salt stress such as effect of phytohormones, osmoprotectant, antioxidants, polyamines and trace elements (Kamran et al., 2019). For instance, treatment of 2-week-old rice plants under 150 mM NaCl stress with sodium selenate (Na2SeO4) improved the total biomass and increased the activity of antioxidant enzyme (Subramanyam et al., 2019) or exogenous melatonin pretreatment mitigates salt stress in wheat seedlings through regulation on polyamine metabolism (Ke et al., 2018). In addition, it has been reported that exogenous application of ascorbic acid could alleviate the adverse effects of salt stress on wheat plant (Athar et al., 2008; Khan et al., 2006). In this research, for the first time we investigate the effects of Faradarmani CF on wheat plant in order to improve plant tolerance to salinity stress. Although, consciousness field is neither matter nor energy, it is possible to trace its effects with various experimental test. For instance, the changes in cancer cell growth (Taheri et al., 2020a) and investigation on the electrical activity of the brain during Faradarmani connection in the Faradarmangar population (Taheri et al., 2020b) are other observations that have used this method in research.
Various abiotic stresses, including salinity, contribute to formation of ROS (Navari-Izzo et al., 1998). Data of this study showed that under salinity conditions there was an increase in H2O2 content with Faradarmani CF treatment, which coincided with an increase in SOD activity (about 220%). Similarly, it has been reported that Static Magnetic Field (SMF), induced H2O2 content and SOD activity in Matricaria chamomilla (Hassanpour & Niknam, 2020). SOD converts superoxide radicals to H2O2 and molecular oxygen. It is possible that increasing H2O2 could therefore be attributed to Faradarmani-induced enhancement of SOD activity. This function may have a key role in mitigating oxidative stress. SOD is the first enzyme involved in antioxidative processes (Rubio et al., 2002). Increasing the activity of SOD was observed similarly in the leaves of sugar beet (Bor et al., 2003) Lycopersicon (Koca et al., 2006), and in wheat (Esfandiari et al., 2007; Sairam et al., 2002) under salt stress. However, under salinity conditions, Faradarmani CF decreased POX activity, which decomposes the H2O2 produced by SOD. Wang et al. (2014) demonstrated that the activity of POX decreased in four wheat cultivars under high temperature (HT) stress and HT-resistant wheat cultivars had higher SOD activity. These results suggest that H2O2 may take part in the signaling networks. It has been reported that seed pretreatment with H2O2 improves salt tolerance of wheat seedlings by alleviation of oxidative damage and expression of stress proteins (Wahid et al., 2007). Additionally, accumulation of H2O2 is thought to be a signal for induction of pathogenesis-related (PR) genes (Chen et al., 1993). Kuźniak & Urbanek (2000) reported that H2O2 contributes to signal transduction, gene expression and cellular defense under oxidative stress conditions.
In the present study, Faradarmani CF also induced PPO activity. PPO may play a key role in scavenging H2O2 in salt-stressed plant. Agarwal & Pondey (2004) found that in Cassia angustifolia PPO activity increased under salinity stress. In wheat, various experiments reported changes in antioxidant enzymes activities to overcome environmental stresses (Agarwal et al., 2005; Barakat, 2011; Heidari, 2009). The mechanism of action of Faradarmani CF as an inducer of antioxidant enzymes activity is not clear; therefore, future studies are needed to gain additional insights on biological and biochemical effects of this CF on various plants under biotic and abiotic stresses.
MDA content, which is a product of lipid peroxidation, reflects membrane destruction under oxidative stresses (Hernández & Almensa, 2002). Increase of MDA content under salt stress have been previously observed in different studies (Hasanuzzaman et al., 2014; Saha et al., 2010; Torabi & Niknam, 2011; Weisany et al., 2012). Mandhania et al., 2006, reported that the better NaCl tolerance in a salt-tolerant cultivar of wheat was associated with lower MDA and much higher activities of antioxidant enzymes compared to salt-sensitive cultivar. Faradarmani as a CF decreased MDA content under salinity stress. It seems that decreased MDA content is correlated with increased activity of antioxidant enzymes under the influence of Faradarmani CF and a strategy developed by plant to withstand salt stress.
From these results, it can be concluded that Faradarmani CF minimizes the negative effects of salt stress in the wheat plant with evidence of increased activity of antioxidant enzymes, increased chlorophyll content and less membrane damage. The main challenge of this study is the fact that Consciousness Field doesn’t possess a quantity and isn’t directly measurable. Therefore, in order to identify its specific effects, we have measured Faradarmani CF effects indirectly on a plant’s biochemical processes. We suggest that other researchers repeat similar experiment with different plants. It seems that botanical bioassays are suitable for screening the effect of such treatments, and apart from the placebo responses by humans, these assays can be beneficial to save time and resources.
Harvard Dataverse: Alleviative Effects of Faradarmani Consciousness Field on Triticum aestivum L. under Salinity Stress, https://doi.org/10.7910/DVN/XNMRMV (Torabi, 2020).
This project contains the following underlying data:
Raw data of chlorophyll a, chlorophyll b, hydrogen peroxide, MDA, POX, PPO, SOD, and total chlorophyll content in control and salinity conditions with and without receiving Faradarmani consciousness field (separate .tab files).
Charts of chlorophyll a, chlorophyll b, hydrogen peroxide, MDA, POX, PPO, SOD, and total chlorophyll content in control and salinity condition with and without receiving Faradarmani consciousness field (separate .docx files).
Data are available under the terms of the Creative Commons Zero "No rights reserved" data waiver (CC0 1.0 Public domain dedication).
1Gaining an announcement: users must register on the COSMOintel Website (free). Once registered, go to the researcher section and fill out the form (the required information is listed below). In order to study at any given time and place, the researchers simply need to introduce the testing center to the guidance center. This means that the exact time of start and finish of the test, the total duration of the test, the number of samples and controls and their contractual name must be specified. It should be noted that registration on the site and also requesting and gaining an announcement is free. We recommend that you contact our center for the definition and selection of the relevant sample in order to obtain a clear and repeatable results (email: researchers@cosmointel.com).
General Condition of Study; Study location; Address; University/center; Research Area: Basic science, Engineering, Medical science, cognitive science, Humanities, others; Sample Name; Number of samples; Control Name; Brief explanation of the experiment; Exact time of research initiation; Exact time of completion of the research
Views | Downloads | |
---|---|---|
F1000Research | - | - |
PubMed Central
Data from PMC are received and updated monthly.
|
- | - |
Is the work clearly and accurately presented and does it cite the current literature?
No
Is the study design appropriate and is the work technically sound?
Partly
Are sufficient details of methods and analysis provided to allow replication by others?
Partly
If applicable, is the statistical analysis and its interpretation appropriate?
Yes
Are all the source data underlying the results available to ensure full reproducibility?
Yes
Are the conclusions drawn adequately supported by the results?
Yes
Competing Interests: No competing interests were disclosed.
Reviewer Expertise: Plant Physiology, primary metabolism, plants stress physiology
Competing Interests: No competing interests were disclosed.
Is the work clearly and accurately presented and does it cite the current literature?
Partly
Is the study design appropriate and is the work technically sound?
No
Are sufficient details of methods and analysis provided to allow replication by others?
No
If applicable, is the statistical analysis and its interpretation appropriate?
Yes
Are all the source data underlying the results available to ensure full reproducibility?
Yes
Are the conclusions drawn adequately supported by the results?
Partly
References
1. Hassanpour H, Niknam V: Establishment and assessment of cell suspension cultures of Matricaria chamomilla as a possible source of apigenin under static magnetic field. Plant Cell, Tissue and Organ Culture (PCTOC). 2020; 142 (3): 583-593 Publisher Full TextCompeting Interests: No competing interests were disclosed.
Alongside their report, reviewers assign a status to the article:
Invited Reviewers | ||
---|---|---|
1 | 2 | |
Version 4 (revision) 23 Jun 23 |
read | |
Version 3 (revision) 17 Mar 21 |
read | |
Version 2 (revision) 02 Dec 20 |
read | |
Version 1 03 Sep 20 |
read |
Provide sufficient details of any financial or non-financial competing interests to enable users to assess whether your comments might lead a reasonable person to question your impartiality. Consider the following examples, but note that this is not an exhaustive list:
Sign up for content alerts and receive a weekly or monthly email with all newly published articles
Already registered? Sign in
The email address should be the one you originally registered with F1000.
You registered with F1000 via Google, so we cannot reset your password.
To sign in, please click here.
If you still need help with your Google account password, please click here.
You registered with F1000 via Facebook, so we cannot reset your password.
To sign in, please click here.
If you still need help with your Facebook account password, please click here.
If your email address is registered with us, we will email you instructions to reset your password.
If you think you should have received this email but it has not arrived, please check your spam filters and/or contact for further assistance.
Hi A. Evans,
I never hide my name and the works I scholarly did! This is superevident that everyone can Google, checking my background. Overall, as an academic ... Continue reading
Hi A. Evans,
I never hide my name and the works I scholarly did! This is superevident that everyone can Google, checking my background. Overall, as an academic and scholar, I believe that scientific and educational areas should welcome novel ideas, including Sciencefact, since we can’t deny the spectacular results and empirical evidence! Let us practice being receptive to new science, which I did so; otherwise, it is getting improved, and we would be lagged.
Best, Mehrnaz
Hi A. Evans,
I never hide my name and the works I scholarly did! This is superevident that everyone can Google, checking my background. Overall, as an academic and scholar, I believe that scientific and educational areas should welcome novel ideas, including Sciencefact, since we can’t deny the spectacular results and empirical evidence! Let us practice being receptive to new science, which I did so; otherwise, it is getting improved, and we would be lagged.
Best, Mehrnaz