YCharOS (Antibody Characterization through Open Science)

YCharOS (Antibody Characterization through Open Science)

Rab GTPase Family

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1-2 of 2 ARTICLES
 in 
https://doi.org/10.12688/f1000research.143928.3
Data Note metrics
Revised
A guide to selecting high-performing antibodies for Rab1A and Rab1B for use in Western Blot, immunoprecipitation and immunofluorescence
[version 3; peer review: 2 approved]
Vera Ruíz Moleón, Charles Alende, Maryam Fotouhi, Riham Ayoubi, Sara González Bolívar, Kathleen Southern, Peter S. McPherson, Carl Laflamme, NeuroSGC/YCharOS/EDDU collaborative group, ABIF consortium
Peer Reviewers Shinsuke Niwa; Claudia Fallini
  • Funders
  • Ontario Genomics
  • Canadian Institutes of Health Research Foundation
  • Mitacs
  • Genome Québec
  • Genome Canada
LATEST VERSION PUBLISHED 07 Mar 2025
https://doi.org/10.12688/f1000research.156209.1
Data Note metrics
A guide to selecting high-performing antibodies for Rab10 (UniProt ID: P61026) for use in western blot, immunoprecipitation, and immunofluorescence
[version 1; peer review: 2 approved]
Vera Ruíz Moleón, Charles Alende, Maryam Fotouhi, Kathleen Southern, Riham Ayoubi, Carl Laflamme, NeuroSGC/YCharOS/EDDU collaborative group, ABIF consortium
Peer Reviewers Jan Voskuil; Sian E. Evans
  • Funders
  • Ontario Genomics
  • Genome Quebec
  • Mitacs
  • Genome Canada
PUBLISHED 17 Sep 2024
1-2 of 2 ARTICLES
Gateway Areas
  • Amyotrophic Lateral Sclerosis (ALS)
    This gateway area serves as a comprehensive resource, filtering the antibody characterization articles for ALS-associated proteins nominated by the Amyotrophic Lateral Sclerosis Reproducible Antibody Platform (ALS-RAP) as well as relevant analyses and research regarding ALS and the ALS-RAP initiative.
      Read more keyboard_arrow_right
  • Alzheimer's Disease (AD)
    This gateway area functions as a complete guide to filter the antibody characterization articles for AD-associated proteins nominated by the TarGet Enablement to Accelerate Therapy Development for AD (TREAT-AD) Center or the Accelerated Medicines Partnership for AD (AMP-AD). It also provides valuable research findings pertaining to AD as well as the analysis of YCharOS’s progress in the context of the targets nominated by these two initiatives. Read more keyboard_arrow_right
  • Parkinson's disease (PD)
    This gateway area presents the antibody characterization data for protein targets nominated by the Michael J. Fox Foundation for Parkinson's Research. Read more keyboard_arrow_right
  • Rab GTPase Family
    This area serves to filter the articles providing antibody characterization data for Rab proteins, a family of over 60 small GTPases, regulating vesicle trafficking processes. Read more keyboard_arrow_right
  • NeuroDisorder Development (NDD) - Autism
    This section highlights articles featuring antibody characterization data for protein targets related to neurodevelopmental disorders (NDD) and autism. The research in this area is partially funded by the Simons Foundation Autism Research Initiative (SFARI).
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Our News
  • YCharOS Consensus Protocols Now Available in Nature Protocols launch

    This collaborative paper, co-authored by representatives from 11 leading antibody manufacturers and knockout cell line providers, details the YCharOS antibody characterization protocols. These protocols outline the use of knockout cell lines to evaluate antibody performance in western blotting, immunoprecipitation, and immunofluorescence. Additionally, the paper offers practical tips and best practices for antibody users.

  • YCharOS highlighted in Nature Protocols News and Views launch

    The YCharOS Consensus Protocols are further spotlighted in a Nature Protocols News and Views article by Monteiro, Voskuil, and Williams, which highlights the YCharOS platform and other complementary efforts in advancing antibody characterization.

  • YCharOS in Nature launch

    The News Feature published in Nature explores a range of initiatives aimed at tackling the antibody reliability problem. The piece spotlights YCharOS as a leading effort in this field, detailing its origins, achievements to date, and future goals.

  • Antibody Specificity: A strong case for third-party testing launch

    Article summarizing the findings from Ayoubi et al,eLife, 2023 that emphasized the extensive use of non-specific antibodies in research and presented a compelling case for third-party antibody testing.

  • Antibody characterization is critical to enhance reproducibility in biomedical research launch

    Overview of the history of the antibody characterization crisis, efforts to address the problem and actions stakeholders can take in order to increase reproducibility of research which rely on antibodies

  • YCharOS in Protocol Exchange launch

    A consensus platform for antibody characterization

  • YCharOS in eLife launch

    Scaling of an antibody validation procedure enables quantification of antibody performance in major research applications

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