YCharOS (Antibody Characterization through Open Science)

YCharOS (Antibody Characterization through Open Science)

Amyotrophic Lateral Sclerosis (ALS)

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Home Gateways YCharOS (Antibody Characterization through Open Science)
1-14 of 14 ARTICLES
 in 
https://doi.org/10.12688/f1000research.169502.1
Data Note metrics AWAITING PEER REVIEW
A guide to selecting high-performing antibodies for VCP (UniProt ID: P55072) for use in western blot, immunoprecipitation, and immunofluorescence
[version 1; peer review: awaiting peer review]
Vera Ruíz Moleón, Maryam Fotouhi, Joel Ryan, Donovan Worrall, Riham Ayoubi, Vincent Francis, Peter S McPherson, Carl Laflamme
Peer Reviewers Invited
  • Funders
  • ALS Society of Canada
  • ALS Association
  • Motor Neurone Disease Association
  • Mitacs
  • Québec Consortium for Drug Discovery
PUBLISHED 09 Sep 2025
https://doi.org/10.12688/f1000research.160371.1
Data Note metrics
A guide to selecting high-performing antibodies for TAF15 (UniProt ID: Q92804) for use in western blot, immunoprecipitation, and immunofluorescence
[version 1; peer review: 2 approved]
Vera Ruíz Moleón, Charles Alende, Maryam Fotouhi, Riham Ayoubi, Carl Laflamme, NeuroSGC/YCharOS/EDDU collaborative group, ABIF consortium
Peer Reviewers Deborah Moshinsky; Sian E. Evans and Carolyn Jones
  • Funders
  • Ontario Genomics
  • ALS Society of Canada
  • Génome Québec
  • ALS Association
  • Motor Neurone Disease Association
  • Mitacs
  • Genome Canada
PUBLISHED 06 Jan 2025
https://doi.org/10.12688/f1000research.160226.1
Data Note metrics
A guide to selecting high-performing antibodies for VAPB (UniProt ID: O95292) for use in western blot, immunoprecipitation, and immunofluorescence
[version 1; peer review: 3 approved, 1 approved with reservations]
Sara González Bolívar, Riham Ayoubi, Charles Alende, Maryam Fothouhi, Irina Shlaifer, Peter S. McPherson, Carl Laflamme, NeuroSGC/YCharOS/EDDU collaborative group, ABIF consortium
Peer Reviewers Mórotz Gábor Miklós; Sheng-ce Tao; Karen Bowman; Jieya Shao
  • Funders
  • Ontario Genomics
  • ALS Society of Canada
  • Canadian Institutes of Health Research Foundation
  • ALS Association
  • Motor Neurone Disease Association
  • Mitacs
  • Genome Quebec
  • Genome Canada
PUBLISHED 24 Dec 2024
https://doi.org/10.12688/f1000research.133220.3
Data Note metrics
Revised
The identification of high-performing antibodies for FUS (Uniprot ID: P35637)  for use in western blot, immunoprecipitation, immunofluorescence and flow cytometry
[version 3; peer review: 2 approved]
Walaa Alshalfie, Michael Biddle, Maryam Fotouhi, Carolyn Jones, Riham Ayoubi, Zhipeng You, Kathleen Southern, Peter S. McPherson, Harvinder Virk, Carl Laflamme, NeuroSGC/YCharOS/EDDU collaborative group
Peer Reviewers Ryota Hikiami; Ralph H. Kehlenbach
  • Funders
  • Ontario Genomics
  • Canadian Institutes of Health Research Foundation
  • ALS Society of Canada
  • Institute for Precision Health and Leicester Drug Discovery and Diagnostics (LD3) via the MRC Impact Accelerator Account
  • ALS Association
  • Motor Neurone Disease Association
  • Genome Quebec
  • Mitacs
  • Genome Canda
LATEST VERSION PUBLISHED 24 Sep 2024
https://doi.org/10.12688/f1000research.132628.2
Data Note metrics
Revised
The identification of high-performing antibodies for Sequestosome-1 for use in Western blot, immunoprecipitation and immunofluorescence
[version 2; peer review: 2 approved]
Riham Ayoubi, Walaa Alshafie, Irina Shlaifer, Kathleen Southern, Peter S. McPherson, Carl Laflamme, NeuroSGC/YCharOS/EDDU collaborative group
Peer Reviewers Yasukazu Takanezawa; Guoqiang Xu
  • Funders
  • Ontario Genomics
  • Canadian Institutes of Health Research Foundation
  • ALS Society of Canada
  • ALS Association
  • Motor Neurone Disease Association
  • Genome Quebec
  • Mitacs
  • Genome Canada
LATEST VERSION PUBLISHED 10 Jul 2024
https://doi.org/10.12688/f1000research.133645.2
Data Note metrics
Revised
A guide to selecting high-performing antibodies for RNA-binding protein TIA1 for use in Western Blot, immunoprecipitation and immunofluorescence
[version 2; peer review: 2 approved]
Maryam Fotouhi, Donovan Worrall, Riham Ayoubi, Kathleen Southern, Peter S. McPherson, Carl Laflamme, NeuroSGC/YCharOS/EDDU collaborative group, ABIF Consortium
Peer Reviewers Claudia Fallini; Jozsef Gal
  • Funders
  • Bristol-Myers Squibb
  • Takeda
  • ALS Society of Canada
  • Pfizer
  • Motor Neurone Disease Association
  • Genome Quebec
  • Genentech
  • Ontario Genomics
  • Canadian Institutes of Health Research Foundation
  • Janssen
  • Bayer AG
  • Boehringer Ingelheim
  • Merck KGaA
  • EUbOPEN
  • ALS Association
  • Mitacs
  • Ontario Genomics Institute
LATEST VERSION PUBLISHED 08 Apr 2024
https://doi.org/10.12688/f1000research.141719.1
Editorial metrics NOT PEER REVIEWED
YCharOS open antibody characterisation data: Lessons learned and progress made
[version 1; peer review: not peer reviewed]
Michael S. Biddle, Harvinder S. Virk
  • Funders
  • Medical Research Council
  • Leicester Institute of Advanced Studies
PUBLISHED 16 Oct 2023
https://doi.org/10.12688/f1000research.132952.2
Data Note metrics
Revised
Identification of high-performing antibodies for Superoxide dismutase [Cu-Zn] 1 (SOD1) for use in Western blot, immunoprecipitation, and immunofluorescence
[version 2; peer review: 2 approved]
Riham Ayoubi, Walaa Alshafie, Zhipeng You, Kathleen Southern, Peter S. McPherson, Carl Laflamme
Peer Reviewers Nishant N Vaikath; Chi Kwan Tsang
  • Funders
  • Ontario Genomics
  • Canadian Institutes of Health Research Foundation
  • ALS Society of Canada
  • ALS Association
  • Motor Neurone Disease Association
  • Genome Quebec
  • Mitacs
  • Genome Canada
LATEST VERSION PUBLISHED 31 Aug 2023
https://doi.org/10.12688/f1000research.133479.2
Data Note metrics
Revised
The identification of high-performing antibodies for Coiled-coil-helix-coiled-coil-helix domain containing protein 10 (CHCHD10) for use in Western Blot, immunoprecipitation and immunofluorescence
[version 2; peer review: 2 approved, 1 approved with reservations]
Riham Ayoubi, Walaa Alshafie, Kathleen Southern, Peter S. McPherson, Carl Laflamme, NeuroSGC/YCharOS/EDDU collaborative group
Peer Reviewers Yang Liu; Derek P. Narendra; Andrew D. Nguyen
  • Funders
  • Ontario Genomics
  • Canadian Institutes of Health Research Foundation
  • Mitacs
  • Genome Quebec
  • Genome Canada
LATEST VERSION PUBLISHED 26 Jul 2023
https://doi.org/10.12688/f1000research.139755.1
Data Note metrics
The identification of high-performing antibodies for Charged multivesicular body protein 2b for use in Western Blot, immunoprecipitation and immunofluorescence
[version 1; peer review: 2 approved]
Walaa Alshafie, Maryam Fotouhi, Riham Ayoubi, Irina Shlaifer, Kathleen Southern, Peter S. McPherson, Carl Laflamme, NeuroSGC/YCharOS/EDDU collaborative group
Peer Reviewers Emma L. Clayton; Xiao-Xin Yan
  • Funders
  • Ontario Genomics
  • Canadian Institutes of Health Research Foundation
  • ALS Society of Canada
  • ALS Association
  • Motor Neurone Disease Association
  • Genome Quebec
  • Mitacs
  • Genome Canada
PUBLISHED 25 Jul 2023
https://doi.org/10.12688/f1000research.131852.2
Data Note metrics
Revised
The identification of high-performing antibodies for TDP-43 for use in Western Blot, immunoprecipitation and immunofluorescence
[version 2; peer review: 2 approved]
Donovan Worrall, Riham Ayoubi, Maryam Fotouhi, Kathleen Southern, Peter S. McPherson, Carl Laflamme, NeuroSGC/YCharOS/EDDU collaborative group, ABIF Consortium
Peer Reviewers Makoto Urushitani; Seiji Watanabe
  • Funders
  • Ontario Genomics
  • Canadian Institutes of Health Research Foundation
  • ALS Society of Canada
  • ALS Association
  • Motor Neurone Disease Association
  • Genome Quebec
  • Mitacs
  • Genome Canada
LATEST VERSION PUBLISHED 20 Jun 2023
https://doi.org/10.12688/f1000research.131851.1
Data Note metrics
The identification of high-preforming antibodies for Ubiquilin-2 for use in Western Blot, immunoprecipitation, and immunofluorescence
[version 1; peer review: 2 approved]
Ian McDowell, Riham Ayoubi, Maryam Fotouhi, Kathleen Southern, Peter S. McPherson, Carl Laflamme, NeuroSGC/YCharOS/EDDU collaborative group, ABIF Consortium
Peer Reviewers Mervyn Monteiro; Carlos Castaneda
  • Funders
  • Ontario Genomics
  • ALS Society of Canada
  • ALS Association
  • Motor Neurone Disease Association
  • Genome Quebec
  • Mitacs
  • Genome Canada
  • Canadian Institutes of Health Research
PUBLISHED 30 Mar 2023
https://doi.org/10.12688/f1000research.132249.1
Data Note metrics
The identification of high-performing antibodies for Profilin-1 for use in Western blot, immunoprecipitation and immunofluorescence
[version 1; peer review: 1 approved, 2 approved with reservations]
Riham Ayoubi, Ian McDowell, Maryam Fotouhi, Kathleen Southern, Peter S. McPherson, Carl Laflamme, NeuroSGC/YCharOS/EDDU collaborative group, ABIF Consortium
Peer Reviewers Partha Roy; Jieya Shao; Xiaoyan Du
  • Funders
  • Ontario Genomics
  • ALS Society of Canada
  • Canadian Institutes of Health Research Foundation
  • ALS Association
  • Motor Neurone Disease Association
  • Mitacs
  • Genome Quebec
  • Genome Canada
PUBLISHED 29 Mar 2023
https://doi.org/10.12688/f1000research.124632.2
Data Note metrics
Revised
Identification of highly specific antibodies for Serine/threonine-protein kinase TBK1 for use in immunoblot, immunoprecipitation and immunofluorescence
[version 2; peer review: 2 approved]
Walaa Alshafie, Maryam Fotouhi, Irina Shlaifer, Riham Ayoubi, Aled M. Edwards, Thomas M. Durcan, Peter S. McPherson, Carl Laflamme
Peer Reviewers Nicolas Charlet; Simon L. Goodman
  • Funders
  • Ontario Genomics
  • Motor Neurone Disease Association (UK)
  • ALS Society of Canada
  • ALS Association
  • Mitacs
  • Genome Canada
  • Canadian Institutes of Health Research
LATEST VERSION PUBLISHED 24 Nov 2022
1-14 of 14 ARTICLES
Gateway Areas
  • Amyotrophic Lateral Sclerosis (ALS)
    This gateway area serves as a comprehensive resource, filtering the antibody characterization articles for ALS-associated proteins nominated by the Amyotrophic Lateral Sclerosis Reproducible Antibody Platform (ALS-RAP) as well as relevant analyses and research regarding ALS and the ALS-RAP initiative.
      Read more keyboard_arrow_right
  • Alzheimer's Disease (AD)
    This gateway area functions as a complete guide to filter the antibody characterization articles for AD-associated proteins nominated by the TarGet Enablement to Accelerate Therapy Development for AD (TREAT-AD) Center or the Accelerated Medicines Partnership for AD (AMP-AD). It also provides valuable research findings pertaining to AD as well as the analysis of YCharOS’s progress in the context of the targets nominated by these two initiatives. Read more keyboard_arrow_right
  • Parkinson's disease (PD)
    This gateway area presents the antibody characterization data for protein targets nominated by the Michael J. Fox Foundation for Parkinson's Research. Read more keyboard_arrow_right
  • Rab GTPase Family
    This area serves to filter the articles providing antibody characterization data for Rab proteins, a family of over 60 small GTPases, regulating vesicle trafficking processes. Read more keyboard_arrow_right
  • NeuroDisorder Development (NDD) - Autism
    This section highlights articles featuring antibody characterization data for protein targets related to neurodevelopmental disorders (NDD) and autism. The research in this area is partially funded by the Simons Foundation Autism Research Initiative (SFARI).
      Read more keyboard_arrow_right
Our News
  • YCharOS Consensus Protocols Now Available in Nature Protocols launch

    This collaborative paper, co-authored by representatives from 11 leading antibody manufacturers and knockout cell line providers, details the YCharOS antibody characterization protocols. These protocols outline the use of knockout cell lines to evaluate antibody performance in western blotting, immunoprecipitation, and immunofluorescence. Additionally, the paper offers practical tips and best practices for antibody users.

  • YCharOS highlighted in Nature Protocols News and Views launch

    The YCharOS Consensus Protocols are further spotlighted in a Nature Protocols News and Views article by Monteiro, Voskuil, and Williams, which highlights the YCharOS platform and other complementary efforts in advancing antibody characterization.

  • YCharOS in Nature launch

    The News Feature published in Nature explores a range of initiatives aimed at tackling the antibody reliability problem. The piece spotlights YCharOS as a leading effort in this field, detailing its origins, achievements to date, and future goals.

  • Antibody Specificity: A strong case for third-party testing launch

    Article summarizing the findings from Ayoubi et al,eLife, 2023 that emphasized the extensive use of non-specific antibodies in research and presented a compelling case for third-party antibody testing.

  • Antibody characterization is critical to enhance reproducibility in biomedical research launch

    Overview of the history of the antibody characterization crisis, efforts to address the problem and actions stakeholders can take in order to increase reproducibility of research which rely on antibodies

  • YCharOS in Protocol Exchange launch

    A consensus platform for antibody characterization

  • YCharOS in eLife launch

    Scaling of an antibody validation procedure enables quantification of antibody performance in major research applications

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