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https://doi.org/10.12688/f1000research.26659.1
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Cloning of human ABCB11 gene in E. coli required the removal of an intragenic Pribnow-Schaller Box before it’s Insertion into genomic safe harbor AAVS1 site using CRISPR–Cas9
[version 1; peer review: 2 approved]
Nisha Vats, Madhusudana Girija Sanal, Senthil Kumar Venugopal, Pankaj Taneja, Shiv Kumar Sarin
Peer Reviewers Mitradas Panicker; Nagendra Chaturvedi
  • Funders
  • Science and Engineering Research Board
  • Department of Biotechnology, Ministry of Science and Technology, India
PUBLISHED 23 Dec 2020
https://doi.org/10.12688/f1000research.27217.2
Opinion Article metrics
Revised
Cancer T-cell therapy: building the foundation for a cure
[version 2; peer review: 3 approved]
Alexander Kamb, William Y. Go
Peer Reviewers John R. James and Muna Fuyal; Barbra J. Sasu; C. Glenn Begley
  • Funder
  • A2 Biotherapeutics, Inc.
LATEST VERSION PUBLISHED 22 Dec 2020
https://doi.org/10.12688/f1000research.20038.2
Research Article metrics
Revised
Predicting the chances of live birth for couples undergoing IVF-ICSI: a novel instrument to advise patients and physicians before treatment
[version 2; peer review: 1 approved with reservations, 2 not approved]
Bruna Estácio da Veiga, Beatriz Brás de Guimarães, Duarte Pedro Tavares, José Luis Metello, Fernando Ferreira, Pedro Ferreira, José Manuel Fonseca
Peer Reviewers Charalampos Siristatidis; Jichun Tan; Edwin Amalraj Raja
  • Funder
  • Portuguese Foundation for Science and Technology
LATEST VERSION PUBLISHED 22 Dec 2020
https://doi.org/10.12688/f1000research.23829.2
Research Article metrics
Revised
Computational screening for potential drug candidates against the SARS-CoV-2 main protease
[version 2; peer review: 2 approved]
Bruno Silva Andrade, Preetam Ghosh, Debmalya Barh, Sandeep Tiwari, Raner José Santana Silva, Wagner Rodrigues de Assis Soares, Tarcisio Silva Melo, Andria Santos Freitas, Patrícia González-Grande, Lucas Sousa Palmeira, Luiz Carlos Junior Alcantara, Marta Giovanetti, Aristóteles Góes-Neto, Vasco Ariston de Carvalho Azevedo
Peer Reviewers Murtaza Tambuwala; Alaa A A Aljabali
  • Funder
  • Conselho Nacional de Desenvolvimento Científico e Tecnológico
LATEST VERSION PUBLISHED 21 Dec 2020
https://doi.org/10.12688/f1000research.27171.1
Software Tool Article metrics
Bleach correction ImageJ plugin for compensating the photobleaching of time-lapse sequences
[version 1; peer review: 4 approved, 1 approved with reservations]
Kota Miura
Peer Reviewers Fabrice P Cordelières; Arianne Bercowsky Rama; Thomas Pengo; Anna H. Klemm; Bram van den Broek
PUBLISHED 21 Dec 2020
https://doi.org/10.12688/f1000research.27019.2
Software Tool Article metrics
Revised
Automated cell tracking using StarDist and TrackMate
[version 2; peer review: 3 approved]
Elnaz Fazeli, Nathan H. Roy, Gautier Follain, Romain F. Laine, Lucas von Chamier, Pekka E. Hänninen, John E. Eriksson, Jean-Yves Tinevez, Guillaume Jacquemet
Peer Reviewers Lachlan W. Whitehead; Ignacio Arganda-Carreras; Vinay Swaminathan
  • Funders
  • Academy of Finland
  • National Cancer Center Finland
  • American Institute for Cancer Research
  • Sigrid Juselius Foundation
  • Medical Research Council
  • Åbo Akademi University Research Foundation
  • National Institutes of Health
  • Cancer Research Institute
LATEST VERSION PUBLISHED 21 Dec 2020
https://doi.org/10.12688/f1000research.26215.1
Research Article metrics
Antibacterial activity in secondary metabolite extracts of heterotrophic bacteria against Vibrio alginolyticus, Aeromonas hydrophila, and Pseudomonas aeruginosa
[version 1; peer review: 2 approved]
Jarod Setiaji, Feli Feliatra, Hilwan Yuda Teruna, Iesje Lukistyowati, Indra Suharman, Zainal Abidin Muchlisin, Teuku Iskandar Johan
Peer Reviewers Agung Damar Syakti; Yuhanis Mhd Bakri
PUBLISHED 21 Dec 2020
https://doi.org/10.12688/f1000research.20916.2
Method Article metrics
Revised
Fast effect size shrinkage software for beta-binomial models of allelic imbalance
[version 2; peer review: 1 approved, 2 approved with reservations]
Joshua P. Zitovsky, Michael I. Love
Peer Reviewers Matthew Stephens; Jarad Niemi and Ignacio Alvarez-Castro; Ernest Turro
  • Funder
  • National Institutes of Health
LATEST VERSION PUBLISHED 14 Dec 2020
https://doi.org/10.12688/f1000research.27262.1
Method Article metrics
A pipeline to analyse time-course gene expression data
[version 1; peer review: 2 approved with reservations]
Nelle Varoquaux, Elizabeth Purdom
Peer Reviewers Michael I. Love; Rodolphe Thiébaut
  • Funders
  • U.S. Department of Energy
  • École Normale Supérieure
  • Gordon and Betty Moore Foundation
  • Alfred P. Sloan Foundation
PUBLISHED 11 Dec 2020
https://doi.org/10.12688/f1000research.22021.4
Research Article metrics
Revised
Effects of polyisoprenoids from Avicennia lanata and Avicennia alba leaves on the gene expression of PI3K, Akt1, mTOR, P53, and EGFR in human colorectal adenocarcinoma WiDr cells
[version 4; peer review: 1 approved, 2 not approved]
Taufiq Qurrohman, Poppy Anjelisa Zaitun Hasibuan, Arif Nuryawan, Sumaiyah Sumaiyah, Etti Sartina Siregar, Mohammad Basyuni
Peer Reviewers Ewa Swiezewska and Liliana Surmacz; Aldes Lesbani; Amos Fatokun
  • Funder
  • Ministry of Research, Technology and Higher Education of the Republic of Indonesia
LATEST VERSION PUBLISHED 09 Dec 2020
https://doi.org/10.12688/f1000research.24808.2
Review metrics
Revised
SMARCB1/INI1-deficient tumors of adulthood
[version 2; peer review: 2 approved]
Nathaniel A. Parker, Ammar Al-Obaidi, Jeremy M. Deutsch
Peer Reviewers Conor Patrick Malone; Abdulqadir Nashwan
LATEST VERSION PUBLISHED 09 Dec 2020
https://doi.org/10.12688/f1000research.25413.2
Research Article metrics
Revised
Biocuration - mapping resources and needs
[version 2; peer review: 2 approved]
Alexandra Holinski, Melissa L. Burke, Sarah L. Morgan, Peter McQuilton, Patricia M. Palagi
Peer Reviewers Tanya Berardini; Luana Licata
  • Funder
  • ELIXIR
LATEST VERSION PUBLISHED 02 Dec 2020
https://doi.org/10.12688/f1000research.24353.2
Case Report metrics
Revised
Case Report: Simultaneously diagnosed gastric adenocarcinoma and pernicious anemia – a classic association
[version 2; peer review: 2 approved, 1 approved with reservations]
Syed Kamran, Mattias K. Dilling, Nathaniel A. Parker, Joel Alderson, Nathan D. Tofteland, Quoc V. Truong
Peer Reviewers Faustina N A. Sackey, Ph.D., FMWI; Talha Badar; Martyn Hooper MBE
LATEST VERSION PUBLISHED 02 Dec 2020
https://doi.org/10.12688/f1000research.25009.2
Research Article metrics
Revised
Immunohistochemical analysis of stem cells from human exfoliated deciduous teeth seeded in carbonate apatite scaffold for the alveolar bone defect in Wistar rats (Rattus novergicus)
[version 2; peer review: 2 approved]
Tania Saskianti, Alexander Patera Nugraha, Chiquita Prahasanti, Diah Savitri Ernawati, Ketut Suardita, Wibi Riawan
Peer Reviewers Niswati Fatmah Rosyida; Lidia Audrey Rocha Valadas
  • Funder
  • Universitas Airlangga
LATEST VERSION PUBLISHED 02 Dec 2020
https://doi.org/10.12688/f1000research.26488.1
Case Report metrics
Case Report: Prostatitis tuberculosis mimicking prostate cancer
[version 1; peer review: 2 approved with reservations]
Syah Mirsya Warli, Dhirajaya Dharma Kadar, Muhammad Haritsyah Warli
Peer Reviewers Dahril Dahril; Zhuo Tony Su
PUBLISHED 18 Nov 2020
https://doi.org/10.12688/f1000research.15666.3
Research Article metrics
Revised
A systematic performance evaluation of clustering methods for single-cell RNA-seq data
[version 3; peer review: 2 approved]
Angelo Duò, Mark D. Robinson, Charlotte Soneson
Peer Reviewers Jean Fan; Saskia Freytag
  • Funders
  • Swiss National Science Foundation
  • Chan Zuckerberg Initiative
LATEST VERSION PUBLISHED 16 Nov 2020
https://doi.org/10.12688/f1000research.24654.2
Research Article metrics
Revised
Investigating a novel multiplex proteomics technology for detection of changes in serum protein concentrations that may correlate to tumor burden
[version 2; peer review: 2 approved]
Annie He Ren, Ioannis Prassas, Antoninus Soosaipillai, Stephanie Jarvi, Steven Gallinger, Vathany Kulasingam, Eleftherios P. Diamandis
Peer Reviewers Georgios Pampalakis; Anna E Lokshin
  • Funder
  • Mount Sinai Hospital
LATEST VERSION PUBLISHED 13 Nov 2020
https://doi.org/10.12688/f1000research.25560.1
Data Note metrics
The complete genome sequences of 22 parrot species (Psittaciformes, Aves)
[version 1; peer review: 3 approved with reservations]
Taylor Hains, Kathleen O'Neill, Jafet Velez, Nancy Speed, Susan Clubb, Taras Oleksyk, Stacy Pirro
Peer Reviewers Paweł Mackiewicz; Devon DeRaad and John McCormack; Roberto Ambrosini and Simona Secomandi
  • Funder
  • Iridian Genomes Inc
PUBLISHED 12 Nov 2020
https://doi.org/10.12688/f1000research.26438.2
Case Report metrics
Revised
Case Report: Complete heart block as a manifestation of cardiac metastasis of oral cancer
[version 2; peer review: 2 approved]
Andrianto Andrianto, Eka Prasetya Budi Mulia, Denny Suwanto, Dita Aulia Rachmi, Mohammad Yogiarto
Peer Reviewers Radityo Prakoso; Pieter A.F.M. Doevendans
LATEST VERSION PUBLISHED 11 Nov 2020
https://doi.org/10.12688/f1000research.22541.1
Research Article metrics
Use of 16s rRNA to identify non-lactose-fermenting bacilli and molecular detection of ESBL resistance genes associated with hospital-acquired infection in Soba University Hospital, Sudan
[version 1; peer review: 1 approved]
Wissam Ahmed Al Hag, Hana Elbadawi, Muzamil Mahdi Abdel Hamid
Peer Reviewers Asim Elnour
PUBLISHED 10 Nov 2020
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