Articles from gateway YCharOS (Antibody Characterization through Open Science)

YCharOS (Antibody Characterization through Open Science)

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The identification of high-performing antibodies for Profilin-1 for use in Western blot, immunoprecipitation and immunofluorescence

[version 1; peer review: 1 approved, 2 approved with reservations]

Riham Ayoubi, Ian McDowell, Maryam Fotouhi, Kathleen Southern, Peter S. McPherson, Carl Laflamme, NeuroSGC/YCharOS/EDDU collaborative group, ABIF Consortium

Peer Reviewers Partha Roy; Jieya Shao; Xiaoyan Du

Funders
Ontario Genomics
ALS Society of Canada
Canadian Institutes of Health Research Foundation
ALS Association
Motor Neurone Disease Association
Mitacs
Genome Quebec
Genome Canada

PUBLISHED 29 Mar 2023

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The identification of high-performing antibodies for transmembrane protein 106B (TMEM106B) for use in Western blot, immunoprecipitation, and immunofluorescence

[version 1; peer review: 1 approved, 2 approved with reservations]

Riham Ayoubi, Maryam Fotouhi, Kathleen Southern, Ritika Bhajiawala, Rebeka Fanti, Panagiotis Prinos, Peter S. McPherson, Carl Laflamme, NeuroSGC/YCharOS/EDDU collaborative group, ABIF Consortium

Peer Reviewers Dawn Smallwood; Christian Peter Moritz; Giuseppe Legname

Funders
Ontario Genomics
Canadian Institutes of Health Research Foundation
ALS Canada
ALS Association
Motor Neurone Disease Association
Genome Quebec
Mitacs
Genome Canada

PUBLISHED 21 Mar 2023

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Revised

Identification of highly specific antibodies for Serine/threonine-protein kinase TBK1 for use in immunoblot, immunoprecipitation and immunofluorescence

[version 2; peer review: 2 approved]

Walaa Alshafie, Maryam Fotouhi, Irina Shlaifer, Riham Ayoubi, Aled M. Edwards, Thomas M. Durcan, Peter S. McPherson, Carl Laflamme

Peer Reviewers Nicolas Charlet; Simon L. Goodman

Funders
Ontario Genomics
Motor Neurone Disease Association (UK)
ALS Society of Canada
ALS Association
Mitacs
Genome Canada
Canadian Institutes of Health Research

LATEST VERSION PUBLISHED 24 Nov 2022

Gateway Areas

Amyotrophic Lateral Sclerosis (ALS)

This gateway area serves as a comprehensive resource, filtering the antibody characterization articles for ALS-associated proteins nominated by the Amyotrophic Lateral Sclerosis Reproducible Antibody Platform (ALS-RAP) as well as relevant analyses and research regarding ALS and the ALS-RAP initiative.
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Alzheimer's Disease (AD)

This gateway area functions as a complete guide to filter the antibody characterization articles for AD-associated proteins nominated by the TarGet Enablement to Accelerate Therapy Development for AD (TREAT-AD) Center or the Accelerated Medicines Partnership for AD (AMP-AD). It also provides valuable research findings pertaining to AD as well as the analysis of YCharOS’s progress in the context of the targets nominated by these two initiatives. Read more
Parkinson's disease (PD)

This gateway area presents the antibody characterization data for protein targets nominated by the Michael J. Fox Foundation for Parkinson's Research. Read more
Rab GTPase Family

This area serves to filter the articles providing antibody characterization data for Rab proteins, a family of over 60 small GTPases, regulating vesicle trafficking processes. Read more
NeuroDisorder Development (NDD) - Autism

This section highlights articles featuring antibody characterization data for protein targets related to neurodevelopmental disorders (NDD) and autism. The research in this area is partially funded by the Simons Foundation Autism Research Initiative (SFARI).
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Our News

YCharOS Consensus Protocols Now Available in Nature Protocols
This collaborative paper, co-authored by representatives from 11 leading antibody manufacturers and knockout cell line providers, details the YCharOS antibody characterization protocols. These protocols outline the use of knockout cell lines to evaluate antibody performance in western blotting, immunoprecipitation, and immunofluorescence. Additionally, the paper offers practical tips and best practices for antibody users.
YCharOS highlighted in Nature Protocols News and Views
The YCharOS Consensus Protocols are further spotlighted in a Nature Protocols News and Views article by Monteiro, Voskuil, and Williams, which highlights the YCharOS platform and other complementary efforts in advancing antibody characterization.
YCharOS in Nature
The News Feature published in Nature explores a range of initiatives aimed at tackling the antibody reliability problem. The piece spotlights YCharOS as a leading effort in this field, detailing its origins, achievements to date, and future goals.
Antibody Specificity: A strong case for third-party testing
Article summarizing the findings from Ayoubi et al,eLife, 2023 that emphasized the extensive use of non-specific antibodies in research and presented a compelling case for third-party antibody testing.
Antibody characterization is critical to enhance reproducibility in biomedical research
Overview of the history of the antibody characterization crisis, efforts to address the problem and actions stakeholders can take in order to increase reproducibility of research which rely on antibodies
YCharOS in Protocol Exchange
A consensus platform for antibody characterization
YCharOS in eLife
Scaling of an antibody validation procedure enables quantification of antibody performance in major research applications

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